I-TASSER results

I-TASSER results for job id Rv3690

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (217 residues)
MPSIDIDREAAHQAAQRELDKPIYPKDSLTKELTDWIDEQLYRILEKGSSIPGGWFTITV
LLILLMIAVTAAVQIARRTMRTNRGGDYQLFDAGQLTAAQHRSTAESYAAEGNWAAAIRH
RLQAVARELEETGMLNPAAGRTANELASDAGEVLPHLAGELTQAATAFNDVTYGERPGTQ
GAYQMIADLDDHLRSRSPAVVSAVQHPAVFDSWAQVR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 \| \| \| \| \| \| \| \| \| \| MPSIDIDREAAHQAAQRELDKPIYPKDSLTKELTDWIDEQLYRILEKGSSIPGGWFTITVLLILLMIAVTAAVQIARRTMRTNRGGDYQLFDAGQLTAAQHRSTAESYAAEGNWAAAIRHRLQAVARELEETGMLNPAAGRTANELASDAGEVLPHLAGELTQAATAFNDVTYGERPGTQGAYQMIADLDDHLRSRSPAVVSAVQHPAVFDSWAQVR
Prediction	CCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	9999999699999999999777678889999999999999999985677888748999999999999999999985565545567665556778899999999999999989999999999999999999889977888999999999999867578899999999999998689889999999999999999985888777778888888887789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 \| \| \| \| \| \| \| \| \| \| MPSIDIDREAAHQAAQRELDKPIYPKDSLTKELTDWIDEQLYRILEKGSSIPGGWFTITVLLILLMIAVTAAVQIARRTMRTNRGGDYQLFDAGQLTAAQHRSTAESYAAEGNWAAAIRHRLQAVARELEETGMLNPAAGRTANELASDAGEVLPHLAGELTQAATAFNDVTYGERPGTQGAYQMIADLDDHLRSRSPAVVSAVQHPAVFDSWAQVR
Prediction	7473617475025104610654415754104301510251044005424723322213222222222223332222332345454555423656513264025204511664303300020022003203754104353421033004303742462354035004202201026351447204303501540573444446546544457425638
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
MPSIDIDREAAHQAAQRELDKPIYPKDSLTKELTDWIDEQLYRILEKGSSIPGGWFTITVLLILLMIAVTAAVQIARRTMRTNRGGDYQLFDAGQLTAAQHRSTAESYAAEGNWAAAIRHRLQAVARELEETGMLNPAAGRTANELASDAGEVLPHLAGELTQAATAFNDVTYGERPGTQGAYQMIADLDDHLRSRSPAVVSAVQHPAVFDSWAQVR

4clvA

0.13

0.09

0.65

1.39

dPPAS2

------------------------------------------------SRTFRLSVSTLVGALVGVLMAIVGVYVTHSTEEPHTSLHEMLHDAVPLDSNEREILELKEEEFTARRREIESRLRAANGKLAESIAKNPQWSPEVEEATREVERAAADLQRATLVHVFEMRAGL------KPEHRAAYDRVLVDALKR---------------------

5cwnA

0.14

0.12

0.86

1.32

dPPAS2

-----MDPEEILERAKESLERAERGDEEEFRKAAEKALELAKRLVEQAKKEGDPELVLEAAKVALRVAELAAKNG----------DKEVFKKAAESALEVAKRLVEVASKEGD-PELVLEAAKVALRVAELAAKNGD------KEVFKKAAESALEVAKRLVEVASKEG---------DPELVEEAAKVAEEVRKLAKKQGDEEVYEKARETAREVK

5cwnA

0.16

0.14

0.89

0.91

MUSTER

-----MDPEEILERAKESLERAR-GDEEEFRKAAEKALELAKRLVEQAKKEGDPELVLEAAKVALRVAELAAKN-----------DKEVFKKAAESALEVAKRLVEVASKEGDLVLEAAKVALRVAELAAKNGDVFKKAAESALEVAKRLVEVASKEPELVEEAAKVAEEVRK-KKQGDEEVYEKARETAREVKEELKRVREEKGGWLEHH------

5cwbA

0.10

0.09

0.89

0.72

dPPAS

------DECEEKARRVAEKVERLKRSGTSEDEIAEEVAREISEVIRTLKESGSSYEVICECVARIVAEIVEALKRS----------GTSEDEIAEIVARVISEVIRTLKESGSSYEVICECVARIVAEIVEALK---RSGTSEDEIAEIVARVISEVIRTLKESGSSYEVIKECVQRIVEEIVEALKRSEDEINEIVRRVKSEVERTLKESGS----

4clvA

0.13

0.09

0.65

0.63

wdPPAS

------------------------------------------------SRTFRLSVSTLVGALVGVLMAIVGVYVTHSTEEPHTSLHEMLHDAVPLDSNEREILELKEEEFTARRREIESRLRAANGKLAESIAKNPQWSPEVEEATREVERAAADLQRATLVHVFEMRAG-------KPEHRAAYDRVLVDALKR---------------------

5cwnA

0.15

0.13

0.89

0.80

wMUSTER

------DPEEILERAKESLERAR-GDEEEFRKAAEKALELAKRLVEQAKKEGDPELVLEAAKVALRVAELAAKNGDKEVFKKAAESA----------LEVAKRLVEVASKEGDLVLEAAKVALRVAELAAKNGDVFKKAAESALEVAKRLVEVASKEPELVEEAAKVAEEVRK-KKQGDEEVYEKARETAREVKEELKRVREEKGGWLEHH------

5thyA1

0.12

0.09

0.75

0.68

wPPAS

LPPLDPVSQQLAPSLTELVTLLDNARSEIGTQLEELSVDYIVQGLLQGW---------------------------------------SYQPTESFDLDAAAQCLG-------VVPTQVRLFERLLQILAEVGILQSNQKVNPSKQSQSLLSQYAATLTLLERCASQLSGVLRGEIDPPQGDLTTATQLYKD---------TIVEKVIKAEKLPPSR

5cwdA

0.12

0.09

0.79

1.31

dPPAS2

---TKEDARSTCEKAARKAAESND------EEVAKQAAKDCLEVAKQAGMPTKEAARSFCEAAARAAAESNDEEVAKIAAKACLEVAKQAGMPTKEAARSFCEAAARAAAESNDEEVAKIAAKACLEVAKQAGMP-----------TKEAARSFCEAAKRAAKESN----------DEEVEKIAKKACKEVAKQAGMPWLE----------------

5cwnA

0.14

0.12

0.88

0.89

PPAS

-----MDPEEILERAKESLERSERGDEEEFRKAAEKALELAKRLVEQAKKEGDPELVLEAAKVALRVAELAAKNGDKEVFKKAAESA----------LEVAKRLVEVASKEGDPELVLEAAKVALRVAELAAKNGDKEVFKKAAESALEVAKRLPELVEEAAKVAEEVRKLAKKQGDVYEKARETAREVKEELKRVREEKGGWLEHH----------

3h4iA2

0.12

0.10

0.80

0.76

Env-PPAS

LPDQRPLSAELEGFLRAG-SPPVY----------------VG--FGSGPAPAE---AARVAIEAVRAQGRRVVLSSGWAGLGRIDEGDDCLVVGEVNHQVLFGRVAAVVHAGTTTAVTRADQPYYAGRVADLGVVAHDGPTTVESLSAALATALPGIRARAAAVAGTI---------RTDGTTVAAKLLLEAISRAKLAAALEHH------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.97

Estimated TM-score = 0.48±0.15

Estimated RMSD = 9.9±4.6Å

Download Model 2

C-score=-2.85

Download Model 3

C-score=-4.32

Download Model 4

C-score=-4.63

Download Model 5

C-score=-4.70

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	5cwnA	0.685	3.20	0.130	0.843
2	5cwmA	0.669	4.05	0.090	0.903
3	5dlqB	0.649	3.51	0.070	0.853
4	3j7jA	0.642	3.39	0.117	0.820
5	4by6A	0.634	4.09	0.047	0.871
6	4uvjA	0.628	4.43	0.071	0.899
7	4fgvA	0.627	4.00	0.053	0.876
8	4kf7A	0.625	4.46	0.068	0.940
9	3s4wA3	0.622	4.37	0.061	0.894
10	4knhA	0.619	4.85	0.073	0.940

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.11	6	1f59B	III	Rep, Mult	116,119,120,123,124,185,186
2	0.06	3	4qi1B	MPG	Rep, Mult	64,67,93,100
3	0.04	2	1fbmA	RTL	Rep, Mult	90,97,100
4	0.04	2	2ie7A	CA	Rep, Mult	140,142
5	0.04	2	3obvC	SUC	Rep, Mult	146,147,150
6	0.02	1	2wy2A	PO3	Rep, Mult	62,120
7	0.02	1	4evtA	URE	Rep, Mult	152,193,197
8	0.02	1	3ue8A	09M	Rep, Mult	140,173
9	0.02	1	3k7tA	GP7	Rep, Mult	52,53
10	0.02	1	2z38A	CL	Rep, Mult	174,178,182
11	0.02	1	4tt0B	IOD	Rep, Mult	98,99,102
12	0.02	1	3t1gA	ZN	Rep, Mult	27,32
13	0.02	1	4rkuL	CLA	Rep, Mult	61,65
14	0.02	1	1iw7P	MG	Rep, Mult	118,122
15	0.02	1	1vdfB	CL	Rep, Mult	97,100
16	0.02	1	2ia5C	MG	Rep, Mult	188,190
17	0.02	1	1f59A	III	Rep, Mult	31,34,35,38,39,89,92,96

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.066	1ujkB	0.458	3.74	0.074	0.622	3.4.23.46	NA
2	0.060	2pflA	0.495	5.18	0.061	0.802	2.3.1.54	184
3	0.060	1e1eB	0.456	5.03	0.045	0.696	3.2.1.21	NA
4	0.060	2vuaA	0.282	6.14	0.035	0.498	3.4.24.69	NA
5	0.060	2cerA	0.502	5.26	0.081	0.793	3.2.1.23	NA
6	0.060	1v03A	0.513	5.13	0.071	0.811	3.2.1.21	NA
7	0.060	1h54B	0.551	4.75	0.075	0.825	2.4.1.8	NA
8	0.060	1r76A	0.517	5.47	0.074	0.839	4.2.2.2	NA
9	0.060	1ayxA	0.540	4.90	0.059	0.825	3.2.1.3	NA
10	0.060	3c5wA	0.527	3.59	0.078	0.705	3.1.3.16	66
11	0.060	1kktA	0.497	4.72	0.079	0.760	3.2.1.113	NA
12	0.060	1gonA	0.518	4.98	0.110	0.788	3.2.1.21	176
13	0.060	1ho8A	0.558	3.75	0.077	0.756	3.6.3.14	95,154,161
14	0.060	2jf6B	0.527	5.07	0.060	0.811	3.2.1.105	NA
15	0.060	1bgaA	0.518	4.92	0.056	0.779	3.2.1.21	NA
16	0.060	1h16A	0.503	5.35	0.061	0.825	2.3.1.54	NA
17	0.060	2gtqA	0.504	4.69	0.080	0.756	3.4.11.2	NA
18	0.060	3eqoA	0.501	5.14	0.079	0.806	3.2.1.58	NA
19	0.060	1gowA	0.501	5.17	0.086	0.783	3.2.1.23	NA
20	0.060	3btaA	0.506	4.86	0.040	0.751	3.4.24.69	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.19	0.604	4.59	0.04	0.91	4uvkA	GO:0000755 GO:0003682 GO:0005634 GO:0005829 GO:0006473 GO:0007064 GO:0009847 GO:0031505 GO:0031618 GO:0031934 GO:0034990 GO:1990707
1	0.18	0.640	3.37	0.06	0.82	4u1dA	GO:0000131 GO:0001731 GO:0002188 GO:0003743 GO:0005737 GO:0005852 GO:0006412 GO:0006413 GO:0006446 GO:0010494 GO:0016282 GO:0033290 GO:0043614
2	0.14	0.642	3.39	0.12	0.82	3j8bA	GO:0001731 GO:0001732 GO:0003743 GO:0005198 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005852 GO:0006412 GO:0006413 GO:0006446 GO:0016020 GO:0016282 GO:0033290 GO:0044822 GO:0071541 GO:0075522 GO:0075525
3	0.10	0.642	3.23	0.06	0.82	5a5tA	GO:0001731 GO:0001732 GO:0003743 GO:0005634 GO:0005730 GO:0005737 GO:0005852 GO:0006412 GO:0006413 GO:0006446 GO:0016020 GO:0016282 GO:0033290 GO:0044822 GO:0071541
4	0.07	0.634	4.09	0.05	0.87	4by6A	GO:0046872
5	0.07	0.592	4.48	0.07	0.84	5fu7A	GO:0000122 GO:0000289 GO:0000932 GO:0004535 GO:0005615 GO:0005634 GO:0005737 GO:0005778 GO:0005829 GO:0006351 GO:0006355 GO:0006417 GO:0006977 GO:0007275 GO:0010606 GO:0016020 GO:0017148 GO:0019904 GO:0030014 GO:0030015 GO:0030331 GO:0031047 GO:0032947 GO:0033147 GO:0035195 GO:0042974 GO:0043231 GO:0044822 GO:0048387 GO:0060213 GO:0061014 GO:0070016 GO:0090503 GO:1900153 GO:2000036
6	0.06	0.427	5.52	0.04	0.71	3renA	GO:0003824 GO:0004553 GO:0005975 GO:0016020 GO:0016021 GO:0016787
7	0.06	0.380	4.74	0.06	0.58	2y4uA	GO:0004860 GO:0005737 GO:0005783 GO:0005788 GO:0005790 GO:0005829 GO:0006417 GO:0006986 GO:0016020 GO:0019901 GO:0031205 GO:0036494 GO:0036498 GO:0043066 GO:0051087 GO:0051603 GO:0051607 GO:0051787 GO:0070062 GO:0070417 GO:1903561 GO:1903912 GO:1904030
8	0.06	0.406	4.90	0.05	0.60	3l0oA	GO:0000166 GO:0003676 GO:0003723 GO:0004386 GO:0005524 GO:0006351 GO:0006353 GO:0006355 GO:0008186 GO:0016787
9	0.06	0.355	5.43	0.04	0.57	4xb1A	GO:0004412 GO:0006520 GO:0016491 GO:0050661 GO:0055114
10	0.06	0.360	5.07	0.04	0.57	3wwpA	GO:0016829 GO:0046872
11	0.06	0.354	4.61	0.04	0.54	1n0rA
12	0.06	0.285	5.94	0.05	0.49	5jj6B	GO:0000166 GO:0005524 GO:0016020 GO:0016021 GO:0016740 GO:0016779 GO:0018117 GO:0070733
13	0.06	0.318	5.04	0.04	0.50	4hea5	GO:0005886 GO:0006810 GO:0008137 GO:0016020 GO:0016491 GO:0016651 GO:0048038 GO:0050136 GO:0055114
14	0.06	0.314	5.68	0.03	0.54	2cuzA	GO:0000049 GO:0000166 GO:0004812 GO:0004818 GO:0005524 GO:0005737 GO:0006412 GO:0006418 GO:0006424 GO:0016874 GO:0016876 GO:0043039
15	0.06	0.311	5.67	0.01	0.53	4p3lA	GO:0006810 GO:0008643 GO:0030288 GO:0042597
16	0.06	0.304	5.45	0.07	0.50	4p7kA	GO:0000287 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006584 GO:0007565 GO:0007612 GO:0007614 GO:0008168 GO:0008171 GO:0008210 GO:0009712 GO:0014070 GO:0016020 GO:0016021 GO:0016036 GO:0016206 GO:0016740 GO:0030424 GO:0030425 GO:0032259 GO:0032496 GO:0032502 GO:0035814 GO:0042135 GO:0042417 GO:0042420 GO:0042493 GO:0043197 GO:0044297 GO:0045211 GO:0045963 GO:0046498 GO:0046500 GO:0046872 GO:0048265 GO:0048609 GO:0048662 GO:0050668 GO:0051930 GO:0070062
17	0.06	0.300	3.96	0.03	0.41	4k51B	GO:0000131 GO:0001731 GO:0002188 GO:0003743 GO:0005737 GO:0005852 GO:0006412 GO:0006413 GO:0006446 GO:0010494 GO:0016282 GO:0033290 GO:0043614
18	0.06	0.281	5.44	0.03	0.42	4k3vB	GO:0006810 GO:0007155 GO:0030001 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0044877	GO:0003743
GO-Score	0.38	0.37
Biological Processes	GO:0002183	GO:0071555	GO:0043543	GO:0000070	GO:0000747	GO:0032502	GO:0071852	GO:0022413	GO:0007062	GO:0006446	GO:0001731
GO-Score	0.46	0.38	0.38	0.38	0.38	0.38	0.38	0.38	0.38	0.37	0.37
Cellular Component	GO:0005721	GO:0000798	GO:0030892	GO:1990421	GO:0005720	GO:0005852	GO:0016282	GO:0033290	GO:0036464	GO:0005829
GO-Score	0.38	0.38	0.38	0.38	0.38	0.37	0.37	0.37	0.36	0.30

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.