I-TASSER results

I-TASSER results for job id Rv3413c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (299 residues)
MREFGNPLGDRPPLDELARTDLLLDALAEREEVDFADPRDDALAALLGQWRDDLRWPPAS
ALVSQDEAVAALRAGVAQRRRARRSLAAVGSVAAALLVLSGFGAVVADARPGDLLYGLHA
MMFNRSRVSDDQIVLSAKANLAKVEQMIAQGQWAEAQDELAEVSSTVQAVTDGSRRQDLI
NEVNLLNTKVETRDPNATLRPGSPSNPAAPGSVGNSWTPLAPVVEPPTPPTPASAAEPSM
SAGVSESPMPNSTSTVAASPSTPSSKPEPGSIDPSLEPADEATNPAGQPAPETPVSPTH

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MREFGNPLGDRPPLDELARTDLLLDALAEREEVDFADPRDDALAALLGQWRDDLRWPPASALVSQDEAVAALRAGVAQRRRARRSLAAVGSVAAALLVLSGFGAVVADARPGDLLYGLHAMMFNRSRVSDDQIVLSAKANLAKVEQMIAQGQWAEAQDELAEVSSTVQAVTDGSRRQDLINEVNLLNTKVETRDPNATLRPGSPSNPAAPGSVGNSWTPLAPVVEPPTPPTPASAAEPSMSAGVSESPMPNSTSTVAASPSTPSSKPEPGSIDPSLEPADEATNPAGQPAPETPVSPTH
Prediction	CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHEEHCCCCCCCCHHHHHHCCCCCCCCCCEEEEHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	99888888888898999987999999977999888997589999999999999866887778985566777777655677777544688999999999974445554689998644567654688777654345456655899999887799888898866665456789988887777666667888888899999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MREFGNPLGDRPPLDELARTDLLLDALAEREEVDFADPRDDALAALLGQWRDDLRWPPASALVSQDEAVAALRAGVAQRRRARRSLAAVGSVAAALLVLSGFGAVVADARPGDLLYGLHAMMFNRSRVSDDQIVLSAKANLAKVEQMIAQGQWAEAQDELAEVSSTVQAVTDGSRRQDLINEVNLLNTKVETRDPNATLRPGSPSNPAAPGSVGNSWTPLAPVVEPPTPPTPASAAEPSMSAGVSESPMPNSTSTVAASPSTPSSKPEPGSIDPSLEPADEATNPAGQPAPETPVSPTH
Prediction	45534467554230530340230031016544244645423201300340265045421453153741332145336444433211000000000000111210001204221102202322154643454423342554154045227545274045305504540551657654653555355245635546454446575446454475455466475365545545535545537545546646447645564563764664564553754553456366376465476365268
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHEEHCCCCCCCCHHHHHHCCCCCCCCCCEEEEHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
MREFGNPLGDRPPLDELARTDLLLDALAEREEVDFADPRDDALAALLGQWRDDLRWPPASALVSQDEAVAALRAGVAQRRRARRSLAAVGSVAAALLVLSGFGAVVADARPGDLLYGLHAMMFNRSRVSDDQIVLSAKANLAKVEQMIAQGQWAEAQDELAEVSSTVQAVTDGSRRQDLINEVNLLNTKVETRDPNATLRPGSPSNPAAPGSVGNSWTPLAPVVEPPTPPTPASAAEPSMSAGVSESPMPNSTSTVAASPSTPSSKPEPGSIDPSLEPADEATNPAGQPAPETPVSPTH

4nl6A

0.14

0.11

0.75

1.13

wPPAS

VPEQEDSGTGQSDDSDI--DD---TAL------------DKAVASF----KHALKNGDICETSGKPKTTPKRKPAKKNKSQKKN-----------------------TAASGDK-----SAIWSEDGC---------PATIASIDFKRETGN--------------REEQNLSD----LSPICEVANNIEQNAQENENESQVSTDESENSRSPGNKSDNIKPKSAPWNSFLPPPPPMPGPRLGPGKPGLKFNGPPPPPPPPPPHLLSCWLPPFPSGPPIIPPPPPICPDSLDDADALGS

1mv3A

0.11

0.05

0.45

1.84

dPPAS2

-------------------------------------------------------------------------------------------------------------------------------------------------------------------DGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLD

5gaoE

0.16

0.15

0.93

1.39

PPAS

---------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

1mv3A

0.11

0.05

0.45

1.04

wPPAS

-------------------------------------------------------------------------------------------------------------------------------------------------------------------DGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLD

4nl6A

0.13

0.10

0.77

1.10

PPAS

VPEQEDSGTGQSDDSDI--DDTALHALKNGDICETSGKPKTTPK-----------RKPAKKNKS------------QKKNTAASL---------------------QQWKVGDK-----SAIWSEDGCIYPATIASIDFKRETCV-------------------TGYGNREEQNLSDLLSPICEVANNIEQNAQENENESQVSTDESENSRSPGNKSDNIKPKSAPWNSFLPPPPPMPGPRLGPGKPGLKFNGPPPPPPPPPPHLLSCWLPPFPSGPPIIPPPPPICPDSLDDADALGS

1mv3A

0.11

0.05

0.45

1.13

PPAS

-------------------------------------------------------------------------------------------------------------------------------------------------------------------DGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLD

5gaoE

0.14

0.09

0.64

1.44

dPPAS2

--------------------------------------------------------------------------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA----------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

3vepX

1.00

0.15

0.96

wPPAS

-----------PPLDELARTDLLLDALAEREEVDFADPRDDALAALLGQWRDDLRW-P-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

3vepX

1.00

0.15

1.30

dPPAS2

-----------PPLDELARTDLLLDALAEREEVDFADPRDDALAALLGQWRDDLRWP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

2mxxA

0.09

0.05

0.60

1.21

dPPAS2

MADEATDAARNND---------------GAYYLQTQFTNADKVNEYLAQHDGEIRAE-----AAADPAVVAAKAALDAVEGGSHNYGEVKAAYEAAFNNAFNAVRNK-------YVQRFQATYNNATEQEGKTYIQGET-------------------PEQANARYLKRVGAANNQNPAAEDKGATTPASKEEAKKSEAAAKNAGKAAGKALPKTSAVKHHHHHH--------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.99

Estimated TM-score = 0.21±0.05

Estimated RMSD = 19.0±2.1Å

Download Model 2

C-score=-5.00

Download Model 3

C-score=-5.00

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4nl6A	0.666	3.57	0.091	0.789
2	4pkcA	0.419	6.56	0.042	0.722
3	2y8nA	0.418	6.40	0.069	0.726
4	2w00B	0.416	6.21	0.029	0.689
5	1vt4I3	0.416	5.63	0.043	0.632
6	4kx7A	0.415	6.20	0.046	0.679
7	4uzsA	0.414	6.46	0.067	0.712
8	3se6A	0.412	6.23	0.036	0.676
9	5ey8A	0.412	6.18	0.069	0.679
10	2f3oA	0.411	7.04	0.043	0.773

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.14	8	4dk4A	CA	Rep, Mult	159,162
2	0.04	2	2xqjA	UUU	Rep, Mult	271,275,279,286,289
3	0.04	2	1p0iA	UUU	Rep, Mult	232,234,237,238
4	0.04	2	2e39A	MAN	Rep, Mult	214,275,276,278
5	0.02	1	2zuvA	NO3	Rep, Mult	186,188,207,208
6	0.02	1	4dlmA	ZN	Rep, Mult	52,79
7	0.02	1	2o01G	CLA	Rep, Mult	260,261
8	0.02	1	1ft6A	SO3	Rep, Mult	151,224
9	0.02	1	4pu3B	NUC	Rep, Mult	51,55
10	0.02	1	2wigA	UUU	Rep, Mult	181,242,243
11	0.02	1	2e3aA	MAN	Rep, Mult	275,276,278
12	0.02	1	1qpaA	MAN	Rep, Mult	255,256,257
13	0.02	1	3djyA	UUU	Rep, Mult	195,196,203,224,225
14	0.02	1	1h78A	DCP	Rep, Mult	265,266,267,268,269,270,273
15	0.02	1	1c8iA	BMA	Rep, Mult	219,275,276
16	0.02	1	5l1gD	GYB	Rep, Mult	153,160
17	0.02	1	1texA	TRE	Rep, Mult	237,297
18	0.02	1	2olnA	NA	Rep, Mult	206,209,210,211

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	5acnA	0.404	6.52	0.058	0.689	4.2.1.3	NA
2	0.060	1gz7C	0.380	6.66	0.068	0.679	3.1.1.3	NA
3	0.060	1uwkA	0.386	5.70	0.059	0.595	4.2.1.49	NA
4	0.060	1lpsA	0.385	6.31	0.096	0.649	3.1.1.3	185
5	0.060	2zuwC	0.405	6.37	0.033	0.699	2.4.1.211	247,264
6	0.060	1cleA	0.383	7.05	0.039	0.716	3.1.1.3	NA
7	0.060	2dqmA	0.385	6.69	0.036	0.679	3.4.11.2	NA
8	0.060	1ry2A	0.386	6.34	0.051	0.655	6.2.1.1	NA
9	0.060	1h16A	0.395	7.02	0.051	0.739	2.3.1.54	NA
10	0.060	1thgA	0.387	6.42	0.104	0.669	3.1.1.3	NA
11	0.060	1maaD	0.408	5.86	0.097	0.639	3.1.1.7	NA
12	0.060	1f6wA	0.350	6.91	0.048	0.649	3.1.1.13,3.1.1.3	NA
13	0.060	1crlA	0.385	6.41	0.105	0.655	3.1.1.3	NA
14	0.060	3ecqB	0.395	6.22	0.030	0.649	3.2.1.97	NA
15	0.060	1n1hA	0.388	6.26	0.018	0.645	2.7.7.48	277
16	0.060	3b7wA	0.385	6.04	0.048	0.632	6.2.1.2	NA
17	0.060	1gz7A	0.391	6.80	0.076	0.712	3.1.1.3	NA
18	0.060	1eveA	0.401	5.89	0.066	0.632	3.1.1.7	NA
19	0.060	2r7oA	0.389	6.01	0.060	0.635	2.7.7.48	181

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.10	0.666	3.57	0.09	0.79	4nl6A	GO:0000245 GO:0000387 GO:0003723 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006353 GO:0006397 GO:0007399 GO:0008380 GO:0015030 GO:0030018 GO:0032797 GO:0034719 GO:0036464 GO:0042802 GO:0043005 GO:0043204 GO:0051170 GO:0097504
1	0.06	0.397	7.01	0.05	0.74	1cm5A	GO:0003824 GO:0005737 GO:0005829 GO:0005975 GO:0006006 GO:0006567 GO:0008152 GO:0008861 GO:0016020 GO:0016740 GO:0016746
2	0.06	0.411	7.04	0.04	0.77	2f3oA	GO:0003824 GO:0008152 GO:0016829
3	0.06	0.370	7.30	0.04	0.73	5bweA	GO:0003824 GO:0008152
4	0.06	0.410	6.55	0.06	0.72	5i2gA	GO:0003824 GO:0008152
5	0.06	0.306	7.15	0.06	0.59	3aw5A	GO:0005507 GO:0016020 GO:0016021 GO:0016491 GO:0016722 GO:0046872 GO:0055114
6	0.06	0.413	6.49	0.06	0.71	5i2aB	GO:0003824 GO:0008152
7	0.06	0.281	6.84	0.06	0.51	4jz7C	GO:0000166 GO:0005829 GO:0006525 GO:0008804 GO:0016301 GO:0016310 GO:0016740 GO:0019546
8	0.06	0.272	6.56	0.03	0.47	3r0xA	GO:0003941 GO:0005737 GO:0006520 GO:0006563 GO:0008721 GO:0016829 GO:0016836 GO:0030170 GO:0030378 GO:0036088 GO:0046416 GO:0070179
9	0.06	0.396	6.92	0.04	0.74	4mtjA	GO:0003824 GO:0008152
10	0.06	0.302	6.71	0.04	0.54	3pyzA	GO:0000166 GO:0004326 GO:0005524 GO:0009058 GO:0009396 GO:0016874 GO:0046901
11	0.06	0.278	5.73	0.07	0.43	2aczB	GO:0005886 GO:0006099 GO:0008177 GO:0009055 GO:0009060 GO:0016020 GO:0016491 GO:0046872 GO:0051536 GO:0051537 GO:0051538 GO:0051539 GO:0055114
12	0.06	0.419	6.50	0.07	0.73	2yajC	GO:0003824 GO:0008152 GO:0016829 GO:0043722
13	0.06	0.270	7.12	0.06	0.50	5einA	GO:0003942 GO:0005737 GO:0006526 GO:0008652 GO:0009085 GO:0016491 GO:0016620 GO:0019878 GO:0046983 GO:0051287 GO:0055114
14	0.06	0.305	6.68	0.04	0.55	2pffA	GO:0000287 GO:0003824 GO:0004312 GO:0004315 GO:0004316 GO:0004321 GO:0005829 GO:0005835 GO:0006629 GO:0006631 GO:0006633 GO:0008152 GO:0008897 GO:0016491 GO:0016740 GO:0042759 GO:0046872 GO:0055114 GO:0102132
15	0.06	0.257	6.65	0.03	0.46	2g77A	GO:0005096 GO:0005794 GO:0005795 GO:0006886 GO:0016192 GO:0017137 GO:0031338 GO:0090630
16	0.06	0.247	6.89	0.03	0.44	5jc7A	GO:0003677 GO:0005524 GO:0016787 GO:0016817
17	0.06	0.256	6.68	0.01	0.45	5btoB	GO:0000463 GO:0000466 GO:0000956 GO:0005634 GO:0005829 GO:0030234 GO:0030846 GO:0031087 GO:0034353 GO:0050790 GO:0071035 GO:0090502 GO:1990174
18	0.06	0.267	7.06	0.06	0.50	2pljA	GO:0003824 GO:0004586 GO:0006596 GO:0016829

Consensus prediction of GO terms

Molecular Function	GO:0003723	GO:0042802	GO:0016829	GO:0008861
GO-Score	0.10	0.10	0.06	0.06
Biological Processes	GO:0000245	GO:0000387	GO:0051170	GO:0007399	GO:0006353	GO:0006567	GO:0006006
GO-Score	0.10	0.10	0.10	0.10	0.10	0.06	0.06
Cellular Component	GO:0044444
GO-Score	0.31

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.