I-TASSER results

I-TASSER results for job id Rv3401

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (786 residues)
MITEDAFPVEPWQVRETKLNLNLLAQSESLFALSNGHIGLRGNLDEGEPFGLPGTYLNSF
YEIRPLPYAEAGYGYPEAGQTVVDVTNGKIFRLLVGDEPFDVRYGELISHERILDLRAGT
LTRRAHWRSPAGKQVKVTSTRLVSLAHRSVAAIEYVVEAIEEFVRVTVQSELVTNEDVPE
TSADPRVSAILDRPLQAVEHERTERGALLMHRTRASALMMAAGMEHEVEVPGRVEITTDA
RPDLARTTVICGLRPGQKLRIVKYLAYGWSSLRSRPALRDQAAGALHGARYSGWQGLLDA
QRAYLDDFWDSADVEVEGDPECQQAVRFGLFHLLQASARAERRAIPSKGLTGTGYDGHAF
WDTEGFVLPVLTYTAPHAVADALRWRASTLDLAKERAAELGLEGAAFPWRTIRGQESSAY
WPAGTAAWHINADIAMAFERYRIVTGDGSLEEECGLAVLIETARLWLSLGHHDRHGVWHL
DGVTGPDEYTAVVRDNVFTNLMAAHNLHTAADACLRHPEAAEAMGVTTEEMAAWRDAADA
ANIPYDEELGVHQQCEGFTTLAEWDFEANTTYPLLLHEAYVRLYPAQVIKQADLVLAMQW
QSHAFTPEQKARNVDYYERRMVRDSSLSACTQAVMCAEVGHLELAHDYAYEAALIDLRDL
HRNTRDGLHMASLAGAWTALVVGFGGLRDDEGILSIDPQLPDGISRLRFRLRWRGFRLIV
DANHTDVTFILGDGPGTQLTMRHAGQDLTLHTDTPSTIAVRTRKPLLPPPPQPPGREPVH
RRALAR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 740 760 780 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MITEDAFPVEPWQVRETKLNLNLLAQSESLFALSNGHIGLRGNLDEGEPFGLPGTYLNSFYEIRPLPYAEAGYGYPEAGQTVVDVTNGKIFRLLVGDEPFDVRYGELISHERILDLRAGTLTRRAHWRSPAGKQVKVTSTRLVSLAHRSVAAIEYVVEAIEEFVRVTVQSELVTNEDVPETSADPRVSAILDRPLQAVEHERTERGALLMHRTRASALMMAAGMEHEVEVPGRVEITTDARPDLARTTVICGLRPGQKLRIVKYLAYGWSSLRSRPALRDQAAGALHGARYSGWQGLLDAQRAYLDDFWDSADVEVEGDPECQQAVRFGLFHLLQASARAERRAIPSKGLTGTGYDGHAFWDTEGFVLPVLTYTAPHAVADALRWRASTLDLAKERAAELGLEGAAFPWRTIRGQESSAYWPAGTAAWHINADIAMAFERYRIVTGDGSLEEECGLAVLIETARLWLSLGHHDRHGVWHLDGVTGPDEYTAVVRDNVFTNLMAAHNLHTAADACLRHPEAAEAMGVTTEEMAAWRDAADAANIPYDEELGVHQQCEGFTTLAEWDFEANTTYPLLLHEAYVRLYPAQVIKQADLVLAMQWQSHAFTPEQKARNVDYYERRMVRDSSLSACTQAVMCAEVGHLELAHDYAYEAALIDLRDLHRNTRDGLHMASLAGAWTALVVGFGGLRDDEGILSIDPQLPDGISRLRFRLRWRGFRLIVDANHTDVTFILGDGPGTQLTMRHAGQDLTLHTDTPSTIAVRTRKPLLPPPPQPPGREPVHRRALAR
Prediction	CCCCCCCCCCCEEEEHCCCCHHHHCCCCEEEEHCCCCEHCCCCCCCCCCCCCCCEEEHCHCCCCCCCCCCCCCCCCCCCCEEEHCCCCCEEEEEHCCEEEHCCCCCEEEEEEEHCCCCEEEEEEEEEEHCCCCEEEEEEEEEEHCCCCEEEEEEEEEEHCCCCEEEEEEEEEHCCCCCCCCCCCCHHHHCCCCCEEEEEEEEHCCEEEEEEEEHCCCEEEEEEEEEEEEHCCCEEEEEHCCCCEEEEEEEEEHCCCEEEEEEEEEEEEHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEHCCCCEHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHEHCCCCCEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEHCCEEEHCCCCCCCCCEEEEEEEHCCEEEEEEEHCCEEEEEEHCCCCCEEEEEHCCEEEEHCCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	998778888986999858887774544434445368655478898877788886688755467877776667887666875765689875899999988757888655589997458769999999995999889999999975788859999999999578689999998758866677788854554567756778776768889999999589869999998999959957999974687799999999959979999999999976899878999999999999998699999999999999999876868689999999999999999998799999998989999989888776655677766887659899999999999999999999999898634378665899888999888755666559999999999997596899999868999999999999855458996998689986668888998745689999999999999999999999989998999999999996889888877977656656777788887777788766666566666655445777777646888899999999999987765799987588999999998998999999999998757788899899865546899999999976574885998998788999898699999998979999998999999997589976999999999998599579999678888888888888766666677779
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 680 700 720 740 760 780 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MITEDAFPVEPWQVRETKLNLNLLAQSESLFALSNGHIGLRGNLDEGEPFGLPGTYLNSFYEIRPLPYAEAGYGYPEAGQTVVDVTNGKIFRLLVGDEPFDVRYGELISHERILDLRAGTLTRRAHWRSPAGKQVKVTSTRLVSLAHRSVAAIEYVVEAIEEFVRVTVQSELVTNEDVPETSADPRVSAILDRPLQAVEHERTERGALLMHRTRASALMMAAGMEHEVEVPGRVEITTDARPDLARTTVICGLRPGQKLRIVKYLAYGWSSLRSRPALRDQAAGALHGARYSGWQGLLDAQRAYLDDFWDSADVEVEGDPECQQAVRFGLFHLLQASARAERRAIPSKGLTGTGYDGHAFWDTEGFVLPVLTYTAPHAVADALRWRASTLDLAKERAAELGLEGAAFPWRTIRGQESSAYWPAGTAAWHINADIAMAFERYRIVTGDGSLEEECGLAVLIETARLWLSLGHHDRHGVWHLDGVTGPDEYTAVVRDNVFTNLMAAHNLHTAADACLRHPEAAEAMGVTTEEMAAWRDAADAANIPYDEELGVHQQCEGFTTLAEWDFEANTTYPLLLHEAYVRLYPAQVIKQADLVLAMQWQSHAFTPEQKARNVDYYERRMVRDSSLSACTQAVMCAEVGHLELAHDYAYEAALIDLRDLHRNTRDGLHMASLAGAWTALVVGFGGLRDDEGILSIDPQLPDGISRLRFRLRWRGFRLIVDANHTDVTFILGDGPGTQLTMRHAGQDLTLHTDTPSTIAVRTRKPLLPPPPQPPGREPVHRRALAR
Prediction	615462152430303254134633322000000000000000000013322100000000013341513431211132212000001002010103434020442403312123002300020202031664340302010000111200000000010243604030201012424143343122012123320332344344310000010342301000000020334352423244443202110203045534010100000000222446402520350044035420440153025103500650504053243002000000000000003324200000000010000000000000000000001020012003001410520251036230300000000020300000000000000000000000000010130230025100200100020000002336613010220100211112030000000000000000000033044006406045601630440062010021674201101120261440315426621102422213014132141000000000031202461132003001300011000000000000010222220030023001000211222021000000000000000000000004621010101006505403010101302020204463020203546624020203645040446451303044552534426315103123235668
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660                 680                 700                 720                 740                 760                 780

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCEEEEHCCCCHHHHCCCCEEEEHCCCCEHCCCCCCCCCCCCCCCEEEHCHCCCCCCCCCCCCCCCCCCCCEEEHCCCCCEEEEEHCCEEEHCCCCCEEEEEEEHCCCCEEEEEEEEEEHCCCCEEEEEEEEEEHCCCCEEEEEEEEEEHCCCCEEEEEEEEEHCCCCCCCCCCCCHHHHCCCCCEEEEEEEEHCCEEEEEEEEHCCCEEEEEEEEEEEEHCCCEEEEEHCCCCEEEEEEEEEHCCCEEEEEEEEEEEEHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEHCCCCEHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHEHCCCCCEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEHCCEEEHCCCCCCCCCEEEEEEEHCCEEEEEEEHCCEEEEEEHCCCCCEEEEEHCCEEEEHCCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCC
MITEDAFPVEPWQVRETKLNLNLLAQSESLFALSNGHIGLRGNLDEGEPFGLPGTYLNSFYEIRPLPYAEAGYGYPEAGQTVVDVTNGKIFRLLVGDEPFDVRYGELISHERILDLRAGTLTRRAHWRSPAGKQVKVTSTRLVSLAHRSVAAIEYVVEAIEEFVRVTVQSELVTNEDVPETSADPRVSAILDRPLQAVEHERTERGALLMHRTRASALMMAAGMEHEVEVPGRVEITTDARPDLARTTVICGLRPGQKLRIVKYLAYGWSSLRSRPALRDQAAGALHGARYSGWQGLLDAQRAYLDDFWDSADVEVEGDPECQQAVRFGLFHLLQASARAERRAIPSKGLTGTGYDGHAFWDTEGFVLPVLTYTAPHAVADALRWRASTLDLAKERAAELGLEGAAFPWRTIRGQESSAYWPAGTAAWHINADIAMAFERYRIVTGDGSLEEECGLAVLIETARLWLSLGHHDRHGVWHLDGVTGPDEYTAVVRDNVFTNLMAAHNLHTAADACLRHPEAAEAMGVTTEEMAAWRDAADAANIPYDEELGVHQQCEGFTTLAEWDFEANTTYPLLLHEAYVRLYPAQVIKQADLVLAMQWQSHAFTPEQKARNVDYYERRMVRDSSLSACTQAVMCAEVGHLELAHDYAYEAALIDLRDLHRNTRDGLHMASLAGAWTALVVGFGGLRDDEGILSIDPQLPDGISRLRFRLRWRGFRLIVDANHTDVTFILGDGPGTQLTMRHAGQDLTLHTDTPSTIAVRTRKPLLPPPPQPPGREPVHRRALAR

1h54B

0.28

0.27

0.94

4.22

MUSTER

M--KRIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGSLQGIYLGGVWYPDKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNRSFVVERGAV-RVALNFQRFLSVAQPELSVQKVTVKNLSAEVDVTLKPSIDADVMNEEANYDE-------RFWDVLATDQQADRGSIVAKTTPNPFRFTSGMEMRLVTDLKNVAITQPNEKEVTTAYTGKLAPQASAELEKRVIVVTSRDYTQESLTAAMHQLSDKVAQSSYEDLLNAHTAIWAQRWEKSDVVIKGDDESQQGIRFNLFQLFSTYYGDARLNIGPKGFTGEKYGGATYWDTEAFAFPVYLGIDPKVTRNLLMYRYKQLDGAYINAQEQGLKGALFPMVTFDGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDDSYVLHEGAKVLTEISRFWADRVHFSKNNQYMIHGVTGADEYENNVDNNWDTNMLAQWTLKYTLEILGKVQDTAKQLDVSDEEKTKWQDIVDRMYLPYDKDLNIFVQHDGFLDKDIEPVSSIPARPINQNWSWDKILRSPYIKQGDVLQGIWDFIDDYTPEQKKANFDFYEPLTVHESSLSPAIHSVLAADLHYEDKAVELYSRTARLDLDNYNNDTTDGLHITSMTGAWIAVVQGFAGMRVRDGQLHYAPFLPKTWTSYTFRQVFRDRLIEVSVHADGPHFKLLSGEP--LTIDVAGAAAAAAAAA--------------------------------

1h54B

0.28

0.26

0.94

6.60

dPPAS

--MKRIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGDLQGIYLGGVWYPDKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNRSFVVER-GAVRVALNFQRFLSVAQPELSVQKVTVKNLSAEVDVTLKPSIDADVMNEEANYDE-------RFWDVLATDQQADRGSIVAKTTPNPPRFTSGMEMRLVTDLKNVAITQPNEKEVTTAYTGKLAPQASAELEKRVIVVTSRDYDTQESLTAAMHQLSDKVASSYEDLLNAHTAIWAQRWEKSDVVIKGDDESQQGIRFNLFQLFSTYYGDARLNIGPKGFTGEKYGGATYWDTEAFAFPVYLGIDPKVTRNLLMYRYKQLDGAYINAQEQGLKGALFPMVTFDGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDDSYVLHEGAKVLTEISRFWADRVHFSKNNQYMIHGVTGADEYENNVDNNWDTNMLAQWTLKYTLEILGKVQDTAKQLDVSDEEKTKWQDIVDRMYLPYDKDLNIFVQHDGFLDKDIEPVSSIPARPINQNWSWDKILRSPYIKQGDVLQGIWDFIDDYTPEQKKANFDFYEPLTVHESSLSPAIHSVLAADLHYEDKAVELYSRTARLDLDNYNNDTTDGLHITSMTGAWIAVVQGFAGMRVRDGQLHYAPFLPKTWTSYTFRQVFRDRLIEVSVHADGPHFKLLSGEP--LTIDVAGAAAAAAAAA--------------------------------

1h54B

0.28

0.26

0.94

8.30

wdPPAS

M--KRIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGSLQGIYLGGVWYPDKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNRSFVVERG-AVRVALNFQRFLSVAQPELSVQKVTVKNLDAEVDVTLKPSIDADVMNEEANYDE-------RFWDVLATDQQADRGSIVAKTTPNPPRFTSGMEMRLVTDLKNVAITQPNEKEVTTAYTGKLAPQASAELEKRVIVVTSRDYDTESLTAAMHQLSDKVAQSSYEDLLNAHTAIWAQRWEKSDVVIKGDDESQQGIRFNLFQLFSTYYGDARLNIGPKGFTGEKYGGATYWDTEAFAFPVYLGIDPKVTRNLLMYRYKQLDGAYINAQEQGLKGALFPMVTFDGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDDSYVLHEGAKVLTEISRFWADRVHFSKNNQYMIHGVTGADEYENNVDNNWDTNMLAQWTLKYTLEILGKVQDTAKQLDVSDEEKTKWQDIVDRMYLPYDKDLNIFVQHDGFLDKDIEPVSSIPARPINQNWSWDKILRSPYIKQGDVLQGIWDFIDDYTPEQKKANFDFYEPLTVHESSLSPAIHSVLAADLHYEDKAVELYSRTARLDLDNYNNDTTDGLHITSMTGAWIAVVQGFAGMRVRDGQLHYAPFLPKTWTSYTFRQVFRDRLIEVSVHADGPHFKLLSGE--PLTIDVAGAAAAAAAAA--------------------------------

1h54B

0.28

0.26

0.94

4.97

wMUSTER

---KRIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGSLQGIYLGGVWYPDKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNRSFVVERGAV-RVALNFQRFLSVAQPELSVQKVTVKNLSAEVDVTLKPSIDADVMNEEANYDERF-------WDVLATDQQADRGSIVAKTTPNPFRFTSGMEMRLVTDLKNVAITQPNEKEVTTAYTGKLAPQASAELEKRVIVVTSRDYDTESLTAAMHQLSDKVAQSSYEDLLNAHTAIWAQRWEKSDVVIKGDDESQQGIRFNLFQLFSTYYGDARLNIGPKGFTGEKYGGATYWDTEAFAFPVYLGIDPKVTRNLLMYRYKQLDGAYINAQEQGLKGALFPMVTFDGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDDSYVLHEGAKVLTEISRFWADRVHFSKNNQYMIHGVTGADEYENNVDNNWDTNMLAQWTLKYTLEILGKVQDTAKQLDVSDEEKTKWQDIVDRMYLPYDKDLNIFVQHDGFLDKDIEPVSSIPARPINQNWSWDKILRSPYIKQGDVLQGIWDFIDDYTPEQKKANFDFYEPLTVHESSLSPAIHSVLAADLHYEDKAVELYSRTARLDLDNYNNDTTDGLHITSMTGAWIAVVQGFAGMRVRDGQLHYAPFLPKTWTSYTFRQVFRDRLIEVSVHADGPHFKLLSGE--PLTIDVAGAAAAAAAAA--------------------------------

1h54B

0.28

0.27

0.94

7.60

wPPAS

M--KRIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGSLQGIYLGGVWYPDKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNRSFVVERGAV-RVALNFQRFLSVAQPELSVQKVTVKNLDAEVDVTLKPSIDADVMNEEANYDER-------FWDVLATDQQADRGSIVAKTTPNPFRFTSGMEMRLVTDLKNVAITQPNEKEVTTAYTGKLAPQASAELEKRVIVVTSRDYDTESLTAAMHQLSDKVAQSSYEDLLNAHTAIWAQRWEKSDVVIKGDDESQQGIRFNLFQLFSTYYGDARLNIGPKGFTGEKYGGATYWDTEAFAFPVYLGIDPKVTRNLLMYRYKQLDGAYINAQEQGLKGALFPMVTFDGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDDSYVLHEGAKVLTEISRFWADRVHFSKNNQYMIHGVTGADEYENNVDNNWDTNMLAQWTLKYTLEILGKVQDTAKQLDVSDEEKTKWQDIVDRMYLPYDKDLNIFVQHDGFLDKDIEPVSSIPARPINQNWSWDKILRSPYIKQGDVLQGIWDFIDDYTPEQKKANFDFYEPLTVHESSLSPAIHSVLAADLHYEDKAVELYSRTARLDLDNYNNDTTDGLHITSMTGAWIAVVQGFAGMRVRDGQLHYAPFLPKTWTSYTFRQVFRDRLIEVSVHADGPHFKLLSGE--PLTIDVAGAAAAAAAAA--------------------------------

1h54B

0.28

0.26

0.94

10.89

dPPAS2

--MKRIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGDLQGIYLGGVWYPDKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNRSFVVER-GAVRVALNFQRFLSVAQPELSVQKVTVKNLSAEVDVTLKPSIDADVMNEEANYDE-------RFWDVLATDQQADRGSIVAKTTPNPPRFTSGMEMRLVTDLKNVAITQPNEKEVTTAYTGKLAPQASAELEKRVIVVTSRDYDTQESLTAAMHQLSDKVASSYEDLLNAHTAIWAQRWEKSDVVIKGDDESQQGIRFNLFQLFSTYYGDARLNIGPKGFTGEKYGGATYWDTEAFAFPVYLGIDPKVTRNLLMYRYKQLDGAYINAQEQGLKGALFPMVTFDGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDDSYVLHEGAKVLTEISRFWADRVHFSKNNQYMIHGVTGADEYENNVDNNWDTNMLAQWTLKYTLEILGKVQDTAKQLDVSDEEKTKWQDIVDRMYLPYDKDLNIFVQHDGFLDKDIEPVSSIPARPINQNWSWDKILRSPYIKQGDVLQGIWDFIDDYTPEQKKANFDFYEPLTVHESSLSPAIHSVLAADLHYEDKAVELYSRTARLDLDNYNNDTTDGLHITSMTGAWIAVVQGFAGMRVRDGQLHYAPFLPKTWTSYTFRQVFRDRLIEVSVHADGPHFKLLSGEP--LTIDVAGAAAAAAAAA--------------------------------

1h54B

0.28

0.27

0.94

6.90

PPAS

M--KRIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGSLQGIYLGGVWYPDKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNRSFVVERGAV-RVALNFQRFLSVAQPELSVQKVTVKNLDAEVDVTLKPSIDADVMNEEANYDE-------RFWDVLATDQQADRGSIVAKTTPNPFRFTSGMEMRLVTDLKNVAITQPNEKEVTTAYTGKLAPQASAELEKRVIVVTSRDYDTQESLTAAMHQLSDKVASSYEDLLNAHTAIWAQRWEKSDVVIKGDDESQQGIRFNLFQLFSTYYGDARLNIGPKGFTGEKYGGATYWDTEAFAFPVYLGIDPKVTRNLLMYRYKQLDGAYINAQEQGLKGALFPMVTFDGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDDSYVLHEGAKVLTEISRFWADRVHFSKNNQYMIHGVTGADEYENNVDNNWDTNMLAQWTLKYTLEILGKVQDTAKQLDVSDEEKTKWQDIVDRMYLPYDKDLNIFVQHDGFLDKDIEPVSSIPARPINQNWSWDKILRSPYIKQGDVLQGIWDFIDDYTPEQKKANFDFYEPLTVHESSLSPAIHSVLAADLHYEDKAVELYSRTARLDLDNYNNDTTDGLHITSMTGAWIAVVQGFAGMRVRDGQLHYAPFLPKTWTSYTFRQVFRDRLIEVSVHADGPHFKLLSGE--PLTIDVAGAAAAAAAAA--------------------------------

1h54B

0.28

0.27

0.94

10.56

Env-PPAS

M--KRIFEVQPWNVITHTFDPKDKRLQESMTSLGNGYMGMRGDFEEGYSGSLQGIYLGGVWYPDKTRVGWWKNGYPKYFGKVVNAVNFIKLPIEINGEPVDLAKDKISDFTLDLDMHQGVLNRSFVVERGAV-RVALNFQRFLSVAQPELSVQKVTVKNLDAEVDVTLKPSIDADVMNEEANYDERF-------WDVLATDQQADRGSIVAKTTPNPFRFTSGMEMRLVTDLKNVAITQPNEKEVTTAYTGKLAPQASAELEKRVIVVTSRDYDTQESLTAAMHQLSDKVASSYEDLLNAHTAIWAQRWEKSDVVIKGDDESQQGIRFNLFQLFSTYGEDARLNIGPKGFTGEKYGGATYWDTEAFAFPVYLGIDPKVTRNLLMYRYKQLDGAYINAQEQGLKGALFPMVTFDGIECHNEWEITFEEIHRNGDIAFAIYNYTRYTGDDSYVLHEGAKVLTEISRFWADRVHFSKNNQYMIHGVTGADEYENNVDNNWDTNMLAQWTLKYTLEILGKVQDTAKQLDVSDEEKTKWQDIVDRMYLPYDKDLNIFVQHDGFLDKDIEPVSSIPARPINQNWSWDKILRSPYIKQGDVLQGIWDFIDDYTPEQKKANFDFYEPLTVHESSLSPAIHSVLAADLHYEDKAVELYSRTARLDLDNYNNDTTDGLHITSMTGAWIAVVQGFAGMRVRDGQLHYAPFLPKTWTSYTFRQVFRDRLIEVSVHADGPHFKLLSGE--PLTIDVAGAAAAAAAAA--------------------------------

4ktpA

0.24

0.22

0.94

4.07

MUSTER

EIGEHLTTNTGWDIIKNRYEAAQAITEGSNFMIGNGFMGYRGTFAEDGKDAYAACIVTDTWDKADGKW-----------EELSTVPNALLTLLHVDGEPFIM-SEEAASFERTLDLSQGVTSRKVSQRMKNGATITIHEEKFASYRKKHAVLMKYTVES-DQDTDAVLDTGIDYDVWSIN-----------GDHLQGHHYFSHPTGDGVTAKTVSYEDTVTVVETCSLDADASEEDYQN--PDGSGRTFPLSLEAGKPVTLEKAMIIYSSNDVDN--PQDEALLEAKHM--QSYEEEKAANRLEWDNLWSHYDVTIQNNIIDQVALRFNIYHAIIATPVHKSLPIGARGLSCQAYQGAAFWDQEIYNMPMYLYSNPEIARNILKYRHRTLDGARRKAKRLGYEGAYYAWISGKGDELCPDFHFNDWQIHISPDIAYAVKKYHQVTGDDAFIRDYGAEMIFEIARFLASHAVYKPRGRYEFMRVQGPDEYHENVDNNAFTNHQAMFTLQAADELLQTLEKTLSAVKLSDDEISLWRDMLANTYVPKPDKHGIIEQFDGYYDLETIIPERLIKEDEYYGYPNGVTVRTQCIKQADVIQLFVLHPHLYDRKTVELNYEFYEPRTLHFSSLSPSSYAIVAAQIDKVEEAYRNFRKSVMIDLLNTNGTFIGGIHTAANGASWQMVVNGFGGLSVHGDDIHLSPRLPDAWDGYTFKAIVKGQTLEVDVTKEQITITNKSEDRKPLTLHIFGEKSVLDSERITKSRLEHHHH---------------------

4ktpA

0.24

0.22

0.94

6.39

dPPAS

EIGEHLTTNTGWDIIKNRYEAAQAITEGSNFMIGNGFMGYRGTFAEDGKDAYAACIVTDTWDKADGKW-----------EELSTVPNALLTLLHVDGEPF-IMSEEAASFERTLDLSQGVTSRKVSQRMKNGATITIHEEKFASYRKKHAVLMKYTVES-DQDTDAVLDTGIDYDVWSIN-----------GDHLQGHHYFSHPTGDGVTAKTVSYEDTVTVVETCSLDADASEEDYQN--PDGSGRTFPLSLEAGKPVTLEKAMIIYSSNDVDN----PQDEALLEAKHMQSYEEEKAANRLEWDNLWSHYDVTIQNNIIDQVALRFNIYHAIIATPVHKSLPIGARGLSCQAYQGAAFWDQEIYNMPMYLYSNPEIARNILKYRHRTLDGARRKAKRLGYEGAYYAWISGTGDELCPDFHFNDWQIHISPDIAYAVKKYHQVTGDDAFIRDYGAEMIFEIARFLASHAVYKMRGRYEFMRVQGPDEYHENVDNNAFTNHQAMFTLQAADELLQTLDEKTLSAVLSDDEISLWRDMLANTYVPKPDKHGIIEQFDGYYDLETIIPAKLIKEDEYYGYPNGVTVRTQCIKQADVIQLFVLHPHLYDRKTVELNYEFYEPRTLHFSSLSPSSYAIVAAQIDKVEEAYRNFRKSVMIDLLNTNEAFIGGIHTAANGASWQMVVNGFGGLSVHGDDIHLSPRLPDAWDGYTFKAIVKGQTLEVDVTKEQITITNKSEDRKPLTLHIFGEKSVLDSERITKSRLEHHHH---------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.99

Estimated TM-score = 0.85±0.08

Estimated RMSD = 6.1±3.8Å

Download Model 2

C-score=-0.67

Download Model 3

C-score=-1.58

Download Model 4

C-score=-2.46

Download Model 5

C-score=0.64

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1h54B	0.936	0.92	0.280	0.944
2	4ktpA	0.897	2.18	0.239	0.935
3	3wiqA	0.864	2.53	0.275	0.911
4	2eabA	0.740	4.61	0.090	0.874
5	4ufcA	0.725	4.17	0.102	0.836
6	2rdyA	0.707	4.45	0.108	0.827
7	2cqtA	0.700	4.82	0.112	0.842
8	3qdeA	0.696	4.78	0.116	0.836
9	1v7vA	0.672	5.17	0.089	0.824
10	4zleA	0.658	5.07	0.109	0.807

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.24	8	4ktpA	BGC	Rep, Mult	347,355,360,361,362,488,590,591,625,627
2	0.19	4	1h54B	PO4	Rep, Mult	355,625,626,669
3	0.03	1	1agmA	MAN	Rep, Mult	329,330,333,672,673
4	0.03	1	1dogA	NOJ	Rep, Mult	34,35,39
5	0.03	1	4ktrA	GOL	Rep, Mult	79,181,354,355,356
6	0.03	1	3c67B	GLC	Rep, Mult	405,457,460,461,464
7	0.03	1	3eqaA	TRS	Rep, Mult	361,362,486
8	0.03	1	4ktrA	GOL	Rep, Mult	12,117,302,306
9	0.03	1	1dogA	MAN	Rep, Mult	143,297,298,299,300,301

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.553	1h54B	0.936	0.92	0.280	0.944	2.4.1.8	488
2	0.103	2eabA	0.740	4.61	0.090	0.874	3.2.1.63	NA
3	0.060	1fo2A	0.317	5.32	0.070	0.400	3.2.1.113	58,410
4	0.060	2vdcA	0.328	8.86	0.039	0.544	1.4.1.13	NA
5	0.060	1l2aE	0.366	5.08	0.086	0.448	3.2.1.4	28
6	0.060	2sqcA	0.340	7.84	0.063	0.518	5.4.99.17	NA
7	0.060	1fp3A	0.326	5.10	0.066	0.401	5.1.3.8	NA
8	0.060	1llwA	0.337	8.72	0.042	0.548	1.4.7.1	NA
9	0.060	2qnoA	0.350	5.19	0.078	0.431	3.2.1.4	NA
10	0.060	2f6dA	0.376	4.68	0.084	0.447	3.2.1.3	NA
11	0.060	1gaiA	0.367	3.84	0.083	0.419	3.2.1.3	46
12	0.060	1lf6A	0.583	4.38	0.077	0.681	3.2.1.3	NA
13	0.060	3ebgA	0.326	8.61	0.035	0.532	3.4.11.-	NA
14	0.060	2cqsA	0.700	4.82	0.122	0.842	2.4.1.20	NA
15	0.060	1nxcA	0.338	6.05	0.061	0.443	3.2.1.113	NA
16	0.060	2g49A	0.324	9.15	0.037	0.553	3.4.24.56	NA
17	0.060	1bxrA	0.343	8.41	0.053	0.543	6.3.5.5	NA
18	0.060	1ea0A	0.339	8.71	0.035	0.553	1.4.1.13	639
19	0.060	2ri9B	0.338	6.18	0.037	0.448	3.2.1.113	NA
20	0.060	2pdaA	0.330	8.36	0.037	0.523	1.2.7.1	NA
21	0.060	2vdcF	0.340	8.68	0.039	0.552	1.4.1.13	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.43	0.936	0.92	0.28	0.94	1h54B	GO:0003824 GO:0005975 GO:0030246
1	0.41	0.869	2.47	0.28	0.92	3wirA	GO:0003824 GO:0005975 GO:0016740 GO:0016757 GO:0030246 GO:0033831
2	0.12	0.725	4.19	0.10	0.84	4ufcB	GO:0003824 GO:0004560
3	0.10	0.696	4.78	0.12	0.84	3qdeA	GO:0003824 GO:0005975 GO:0016740 GO:0016757 GO:0030246 GO:0047738
4	0.06	0.232	5.62	0.06	0.29	3ly8A	GO:0000160 GO:0003677 GO:0005622 GO:0005886 GO:0006351 GO:0006355 GO:0016020 GO:0016021 GO:0045893
5	0.06	0.175	7.94	0.04	0.27	1sk8A	GO:0003993 GO:0005576 GO:0016158 GO:0016311 GO:0016787 GO:0016791
6	0.06	0.199	6.59	0.06	0.28	4e2pA	GO:0004497 GO:0005506 GO:0016491 GO:0016705 GO:0020037 GO:0046872 GO:0055114
7	0.06	0.191	6.41	0.05	0.26	4c0aB	GO:0005085 GO:0005086 GO:0005634 GO:0005737 GO:0008289 GO:0016020 GO:0030036 GO:0032012 GO:0043547
8	0.06	0.190	6.85	0.04	0.27	3v3wA	GO:0000287 GO:0003824 GO:0008152 GO:0008927 GO:0009063 GO:0016052 GO:0016829 GO:0046872
9	0.06	0.167	6.77	0.05	0.23	4f9vA	GO:0005576 GO:0016603 GO:0016740 GO:0016746 GO:0017186 GO:0046872
10	0.06	0.193	7.20	0.03	0.28	4hzvA	GO:0004308 GO:0005975 GO:0008152 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0019012 GO:0020002 GO:0033644 GO:0046872 GO:0052794 GO:0052795 GO:0052796 GO:0055036
11	0.06	0.179	6.41	0.06	0.24	4mhnA	GO:0016603 GO:0016740 GO:0016746 GO:0046872
12	0.06	0.177	6.32	0.04	0.24	4wy9A	GO:0004871 GO:0007165 GO:0016020 GO:0016021
13	0.06	0.143	7.19	0.05	0.21	2jfnA	GO:0006807 GO:0008152 GO:0008360 GO:0008881 GO:0009252 GO:0016853 GO:0016855 GO:0036361 GO:0071555
14	0.06	0.127	5.25	0.06	0.16	1ynaA	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0031176 GO:0045493
15	0.06	0.124	5.06	0.04	0.15	1pvxA	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0031176 GO:0045493
16	0.06	0.103	4.86	0.04	0.12	4g3kA	GO:0000166 GO:0003677 GO:0005524 GO:0006351 GO:0006355 GO:0008134 GO:0043565
17	0.06	0.111	6.06	0.06	0.15	3gydB	GO:0000166
18	0.06	0.127	5.81	0.03	0.17	1z81A	GO:0000413 GO:0003755 GO:0006457 GO:0016853

Consensus prediction of GO terms

Molecular Function	GO:0030246	GO:0033831
GO-Score	0.70	0.41
Biological Processes	GO:0005975
GO-Score	0.70
Cellular Component	GO:0005886	GO:0005622	GO:0016021
GO-Score	0.06	0.06	0.06

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.