I-TASSER results

I-TASSER results for job id Rv3395A

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (208 residues)
MQSRKTTSVLAAALLFCGLLGPGTAPPATGGGPACRPAELFATDNTTDGFELPAVATIAL
TGTVVTGSTLVDGVFWSNERQQIGYERSREFHLCVVDAPTLHNAAEALHRQFNQEAVLTF
DYLPQNAPEADAILITVPDIGIARFRDAFASDLAAHHRLRGGSVTTADHTLILVAGNGDL
DVARRLVEEAGGDWNATTIAHGRREFVN

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 \| \| \| \| \| \| \| \| \| \| MQSRKTTSVLAAALLFCGLLGPGTAPPATGGGPACRPAELFATDNTTDGFELPAVATIALTGTVVTGSTLVDGVFWSNERQQIGYERSREFHLCVVDAPTLHNAAEALHRQFNQEAVLTFDYLPQNAPEADAILITVPDIGIARFRDAFASDLAAHHRLRGGSVTTADHTLILVAGNGDLDVARRLVEEAGGDWNATTIAHGRREFVN
Prediction	CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHCCCCCHHHHHHHHHEEEEHCCHHHHHHHHHHHHHHHCCCEEEEEEHCCCCCCCCCEEEEHCCCCCHHHHHHHHHHCHHHHHHHCCCCEEHCCCEEEEEHCCCCCHHHHHHHHHHCCCCCCEEEEHCCHCCCC
Conf.Score	9766789999999999874688888887888888886467765889996457899999875776666556744787567546677765434455568778999999999997775467777679999655579998599875888888886989999874886565798899997676547899999998886554577645434469
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 \| \| \| \| \| \| \| \| \| \| MQSRKTTSVLAAALLFCGLLGPGTAPPATGGGPACRPAELFATDNTTDGFELPAVATIALTGTVVTGSTLVDGVFWSNERQQIGYERSREFHLCVVDAPTLHNAAEALHRQFNQEAVLTFDYLPQNAPEADAILITVPDIGIARFRDAFASDLAAHHRLRGGSVTTADHTLILVAGNGDLDVARRLVEEAGGDWNATTIAHGRREFVN
Prediction	7644432200000011012123443443546554143130113543355143402321344344443332020011155355332531451313314473034005203641544110004203663442310304044142440340024344135413413133463200001426516103400651333054240432454338
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHCCCCCHHHHHHHHHEEEEHCCHHHHHHHHHHHHHHHCCCEEEEEEHCCCCCCCCCEEEEHCCCCCHHHHHHHHHHCHHHHHHHCCCCEEHCCCEEEEEHCCCCCHHHHHHHHHHCCCCCCEEEEHCCHCCCC
MQSRKTTSVLAAALLFCGLLGPGTAPPATGGGPACRPAELFATDNTTDGFELPAVATIALTGTVVTGSTLVDGVFWSNERQQIGYERSREFHLCVVDAPTLHNAAEALHRQFNQEAVLTFDYLPQNAPEADAILITVPDIGIARFRDAFASDLAAHHRLRGGSVTTADHTLILVAGNGDLDVARRLVEEAGGDWNATTIAHGRREFVN

1g6hA

0.11

0.96

0.74

MUSTER

ILRTENIVKYFGEFKALDGVSISVNKGDVTLIIGPNGSGKSTLINVITGFLKADEGRVYFENKDITNKE-------PAELYHYGIVRTFQTPQPLKEMTVLENLLIGEI--CPGESPLNSLFYKKWIPKEEEMVEKAFKLKLSHLYDRKAGEGGQMKLVEIGRALMTNPKMIVMDEPGLAHDIFNHVLELKAKGITFLIIEHRLDIVL

3e05D

0.13

0.11

0.79

0.53

dPPAS

------------------------------YPVIGIDDDEFATAKKLIT-KQEVRAVTLSKLRLQDDLVMWDIGAGSASVSIEASNLMPNGRIFALERPQYLGFIRDNLKKFVARNVTLVEEGLDDLPDPDRVFIGGSGGMLEEIIDAVDRRLK------------SEGVIVLNAVTLDLTKAVEFLEDHGYMVEVACVNVAKTKGLT

4pscA

0.13

0.11

0.82

0.57

wdPPAS

MPIGDTITAACDLISDGEDAAASLFGISETENDPCGDVTVLFARGTCDP-PWFFDSLQTALGS---RTLGVKGVPYPAS--VQDFLSG--------SVQNGINMANQIKSVLQS-------------PNT---KLVLGGYSSMVVHNAASNDAATMSKISA--VLFGDPY----KPVANFDAAK-NICQGGDIILLPHLTYAE-AFVV

1g6hA

0.10

0.94

0.67

wMUSTER

ILRTENIVKYFGEFKALDGVSISVNKGDVTLIIGPNGSGKSTLINVITGFLKADEGRVYFENKDITNKE-------PAELYHYGIVRTFQTPQPLKEMTVLENLLIGEICP-GESPLNSL-FYKKWIPKEEEMVEKA--LKLSHLYDRKAGEGGQMKLVEIGRALMTNPKMIVMDE-GLAHDIFNHVLELKAKGITFLIIEHRLDIVL

4pscA

0.14

0.11

0.82

0.56

wPPAS

PIGDTITAACDLISDGEDAAASLFGISETENDP-CGDVTVLFARGTCDP-PWFFDSLQTALGS---RTLGVKGVPYPA--SVQDFLSG--------SVQNGINMANQIKSVLQS-------------PNT---KLVLGGYSSMVVHNAASNDAATMSKISA--VLFGDPY----KPVANFDAAK-NICQGGDIILLPHLTYAEDAFVV

3slkA1

0.14

0.11

0.78

0.62

dPPAS2

--------------------ARWPTVQLPTSAQP-PSCVLLGTSEVSADIDLRSLTAALDAGAEPPG-----VVIAPTPPGGGRTADVRE-----TTRHALDLVQGWLSDQRLNESRLLLVT--QGAVAVE------PGEPVTDLAQAALW-GLLRSTQ-----TEHPDRFVLVDVPEPAQLLPALPGVLACGEPQLALRRGGA-HAP

3slkA1

0.13

0.10

0.75

0.64

PPAS

--------------------ARWPTVQLPTSAQP-PSCVLLGTSEVSADIDLRSLTAALDAGAEPPG-----VVIAPTPPGGGRTADVRE-----TTRHALDLVQGWLSDQRLNESRLLLVT--QGAVAVE------PGEPVTDLAQAALW-STQTEH----------DRFVLVDVPEPAQLLPALPGVLACGEPQLALRRGGA-HAP

3tr5A2

0.17

0.11

0.64

0.69

Env-PPAS

--------------------GIPNFAS-----------ELFRLVRLKDPLLLKGLTQLSEEGAT--------QLFRPLD--------SNELILGAVGLLQFDVVAYRLENEYNVK--CVYESVNVVT----ARWVICD--DKAVLERFNQEQSRNL-------AYDGGGHLTYLAPSRNLEITEKW----------PEIQFSEREH--

1g6hA2

0.09

0.07

0.77

0.66

MUSTER

ILRTENIVKYFGEFKALDGVSISVNKGDVTLIIGPNGSGKSTLINVITGFLKADEGRVYFENKDITNKE-------PAELYHYGIVRTKMIVMDEPIAGVAPGLAHDIFNHVL-----------ELKAKGITFLIIEHRLDVLNYIDHLYV-------MFNGQIIAEGRGE---------EEIKNVLSD--------KVVYGE-----

4bs9A4

0.09

0.07

0.80

0.53

dPPAS

AGKGIREGIVANLAAALEEAGIQVSDPT-------AQLQVVLTDDYLQPELAAINKEALERQQPPVGSILWLGPLFVPGETHCLAQRLQG------NREVEASVLQQKRALQER---------PTARATLPSTLQTGLQWAATEIAKWMVKRHLNAIAPGTARFPTLAGKIFTFN-QTTLELKAHPLSR-------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.91

Estimated TM-score = 0.29±0.09

Estimated RMSD = 14.9±3.6Å

Download Model 2

C-score=-2.93

Download Model 3

C-score=-4.53

Download Model 4

C-score=-4.12

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4fp9A	0.618	4.40	0.089	0.909
2	3g8bB	0.604	3.99	0.086	0.841
3	5e71A	0.598	3.88	0.086	0.822
4	1jg1A	0.591	4.09	0.101	0.812
5	2yxeA	0.583	4.09	0.097	0.803
6	1xdzA	0.581	4.01	0.071	0.812
7	1u7hA	0.580	3.93	0.075	0.803
8	1vllA	0.577	4.06	0.070	0.808
9	2i99A	0.575	3.99	0.076	0.803
10	4go1A	0.575	4.49	0.086	0.851

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.16	7	2e7iB	SO4	Rep, Mult	53,54,55,200,202
2	0.05	2	4eyeA	IOD	Rep, Mult	54,198,199,200
3	0.02	1	3bk0B	CNU	Rep, Mult	44,46
4	0.02	1	1yt3A	ZN	Rep, Mult	121,179
5	0.02	1	3n12A	ZN	Rep, Mult	79,129
6	0.02	1	1y9aC	MG	Rep, Mult	67,131
7	0.02	1	2cl5B	BIE	Rep, Mult	15,16,19,53,86
8	0.02	1	3fytC	XYP	Rep, Mult	205,206
9	0.02	1	3hl2A	PLR	Rep, Mult	53,54,55,75,79,82,83,175,176,203
10	0.02	1	2vk6A	MG	Rep, Mult	139,194
11	0.02	1	4ej7B	CA	Rep, Mult	139,140,205
12	0.02	1	3u7eB	MG	Rep, Mult	44,152
13	0.02	1	1yrhC	FMN	Rep, Mult	147,169
14	0.02	1	1nuiA	MG	Rep, Mult	89,193
15	0.02	1	2xecB	CA	Rep, Mult	89,115

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.066	3e05B	0.563	3.84	0.101	0.750	2.1.1.132	NA
2	0.066	1f38A	0.555	3.27	0.113	0.702	2.1.1.-	NA
3	0.066	1jg3B	0.589	4.05	0.101	0.812	2.1.1.77	136,200
4	0.066	1i1nA	0.570	4.21	0.117	0.798	2.1.1.77	NA
5	0.066	3hnrA	0.507	3.80	0.061	0.697	2.1.1.-	NA
6	0.066	3bwmA	0.562	4.10	0.077	0.784	2.1.1.6	NA
7	0.066	1nt2A	0.532	4.08	0.049	0.755	2.1.1.-	NA
8	0.060	2et6A	0.551	4.18	0.035	0.788	1.1.1.-	NA
9	0.060	3bt7B	0.574	3.73	0.062	0.779	2.1.1.35	NA
10	0.060	2w37A	0.562	4.82	0.080	0.865	2.1.3.3	24
11	0.060	3grzB	0.553	3.92	0.091	0.774	2.1.1.-	NA
12	0.060	1duvG	0.558	4.67	0.096	0.846	2.1.3.3	NA
13	0.060	1acmA	0.555	4.63	0.062	0.837	2.1.3.2	11
14	0.060	1at1A	0.562	4.56	0.052	0.827	2.1.3.2	NA
15	0.060	1ml4A	0.559	4.71	0.056	0.846	2.1.3.2	36
16	0.060	2pbfA	0.570	4.16	0.085	0.798	2.1.1.77	NA
17	0.060	3duwA	0.551	4.17	0.088	0.784	2.1.1.-	132
18	0.060	2yxeA	0.583	4.09	0.097	0.803	2.1.1.77	NA
19	0.060	2cl5A	0.563	4.05	0.072	0.784	2.1.1.6	NA
20	0.060	2b9eA	0.567	3.79	0.087	0.774	2.1.1.-	NA
21	0.060	1r18A	0.565	4.39	0.084	0.808	2.1.1.77	NA
22	0.060	1jg1A	0.591	4.09	0.101	0.812	2.1.1.77	NA
23	0.060	1u7hA	0.580	3.93	0.075	0.803	4.3.1.12	NA
24	0.060	3e05D	0.562	3.69	0.103	0.740	2.1.1.132	54
25	0.060	2be7A	0.557	4.68	0.046	0.841	2.1.3.2	NA
26	0.060	2vs1A	0.563	3.48	0.102	0.745	2.1.1.-	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.21	0.591	4.09	0.10	0.81	1jg1A	GO:0004719 GO:0005737 GO:0006464 GO:0006479 GO:0008168 GO:0016740 GO:0030091 GO:0032259
1	0.19	0.560	4.18	0.08	0.79	3cjtA	GO:0005737 GO:0006479 GO:0008168 GO:0008276 GO:0016740 GO:0032259
2	0.08	0.604	3.99	0.09	0.84	3g8bB	GO:0005737 GO:0006364 GO:0008168 GO:0008649 GO:0016740 GO:0031167 GO:0032259 GO:0070043 GO:0070476
3	0.08	0.523	3.44	0.09	0.68	4iscA	GO:0008168 GO:0008757 GO:0009312 GO:0009877 GO:0016740 GO:0032259
4	0.07	0.622	4.30	0.08	0.91	4fzvA	GO:0003723 GO:0005739 GO:0005762 GO:0006364 GO:0008168 GO:0016740 GO:0019843 GO:0031167 GO:0032259 GO:0042254 GO:0042256 GO:0070131 GO:0070181
5	0.07	0.581	4.01	0.07	0.81	1xdzA	GO:0005737 GO:0006364 GO:0008168 GO:0008649 GO:0016740 GO:0031167 GO:0032259 GO:0070043 GO:0070476
6	0.07	0.573	4.18	0.10	0.80	4o29A	GO:0004719 GO:0005737 GO:0006464 GO:0006479 GO:0008168 GO:0016740 GO:0030091 GO:0032259
7	0.07	0.570	4.16	0.09	0.80	2pbfA	GO:0004719 GO:0006464 GO:0006479 GO:0008168 GO:0016740 GO:0030091 GO:0032259
8	0.07	0.558	4.39	0.08	0.81	1sqfA	GO:0003723 GO:0005737 GO:0005829 GO:0006355 GO:0006364 GO:0008168 GO:0008649 GO:0009383 GO:0016434 GO:0016740 GO:0031167 GO:0032259 GO:0070475
9	0.07	0.569	4.17	0.14	0.79	3lbfA	GO:0004719 GO:0005737 GO:0006464 GO:0006479 GO:0008168 GO:0016740 GO:0030091 GO:0032259
10	0.07	0.583	4.09	0.10	0.80	2yxeA	GO:0004719 GO:0005737 GO:0006464 GO:0006479 GO:0008168 GO:0016740 GO:0030091 GO:0032259
11	0.07	0.570	4.21	0.12	0.80	1i1nA	GO:0004719 GO:0005737 GO:0005783 GO:0005829 GO:0005913 GO:0006464 GO:0006479 GO:0008168 GO:0016740 GO:0030091 GO:0032259 GO:0070062 GO:0098609 GO:0098641 GO:1903561
12	0.07	0.545	3.99	0.09	0.75	5c1iB	GO:0008033 GO:0008168 GO:0016429 GO:0016740 GO:0030488 GO:0031515 GO:0032259
13	0.07	0.558	4.04	0.08	0.78	4xucA	GO:0000287 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006584 GO:0007565 GO:0007612 GO:0007614 GO:0008168 GO:0008171 GO:0008210 GO:0009712 GO:0014070 GO:0016020 GO:0016021 GO:0016036 GO:0016206 GO:0016740 GO:0030424 GO:0030425 GO:0032259 GO:0032496 GO:0032502 GO:0035814 GO:0042135 GO:0042417 GO:0042420 GO:0042493 GO:0043197 GO:0044297 GO:0045211 GO:0045963 GO:0046872 GO:0048265 GO:0048609 GO:0048662 GO:0050668 GO:0051930 GO:0070062
14	0.07	0.563	3.84	0.10	0.75	3e05B	GO:0006479 GO:0008152 GO:0008168 GO:0008276 GO:0009236 GO:0016740 GO:0032259
15	0.07	0.579	3.63	0.10	0.77	2bh2A	GO:0003723 GO:0005506 GO:0006364 GO:0006396 GO:0008168 GO:0008173 GO:0016740 GO:0031167 GO:0032259 GO:0046872 GO:0051536 GO:0051539 GO:0070041 GO:0070475
16	0.07	0.545	4.50	0.11	0.82	3m4xA	GO:0003723 GO:0005737 GO:0006364 GO:0008168 GO:0008757 GO:0016740 GO:0032259
17	0.07	0.547	4.29	0.07	0.78	3m6uB	GO:0003723 GO:0005737 GO:0006364 GO:0008168 GO:0016740 GO:0032259
18	0.07	0.562	4.55	0.07	0.84	3a4tA	GO:0003723 GO:0005737 GO:0006364 GO:0008033 GO:0008168 GO:0008757 GO:0016428 GO:0016740 GO:0030488 GO:0032259
19	0.07	0.567	3.79	0.09	0.77	2b9eA	GO:0003723 GO:0005634 GO:0005730 GO:0006364 GO:0008168 GO:0016740 GO:0032259 GO:0044822
20	0.07	0.554	4.52	0.09	0.82	3m6wA	GO:0003723 GO:0005737 GO:0006364 GO:0008168 GO:0016740 GO:0032259
21	0.07	0.495	3.43	0.08	0.66	3i9fB	GO:0008152 GO:0008168 GO:0032259 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0051998	GO:0008757
GO-Score	0.41	0.41
Biological Processes	GO:0006479
GO-Score	0.36
Cellular Component	GO:0005737
GO-Score	0.41

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.