I-TASSER results

I-TASSER results for job id Rv3344c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (484 residues)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Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| AQASPAAHGGSGGAGGNGGAGSAGNGGAGGAGGNGGAGGNGGGGDAGNAGSGGNGGKGGDGVGPGSTGGAGGKGGAGANGGSSNGNARGGNAGNGGHGGAGGSGDTGGAGGAGGQGGFGGTGGSGSGIGGGAGGNGGNGGAGGTGVVLGGKGGDGGNGDHGGPATNPGSGSRGGAGGSGGNGGAGGNATGSGGKGGAGGNGGDGSFGATSGPASIGVTGAPGGNGGKGGAGGSNPNGSGGDGGKGGNGGAGGNGGSIGANSGIVGGSGGAGGAGGAGGNGSLSSGEGGKGGDGGHGGDGVGGNSSVTQGGSGGGGGAGGAGGSGFFGGKGGFGGDGGQGGPNGGGTVGTVAGGGGNGGVGGRGGDGVFAGAGGQGGLGGQGGNGGGSTGGNGGLGGAGGGGGNAPDGGFGGNGGKGGQGGIGGGTQSATGLGGDGGDGGDGGNGGNSGAKAGGAGGKGQAGQPNSGTEPGFGGDGGLGGAGATP
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	9999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| AQASPAAHGGSGGAGGNGGAGSAGNGGAGGAGGNGGAGGNGGGGDAGNAGSGGNGGKGGDGVGPGSTGGAGGKGGAGANGGSSNGNARGGNAGNGGHGGAGGSGDTGGAGGAGGQGGFGGTGGSGSGIGGGAGGNGGNGGAGGTGVVLGGKGGDGGNGDHGGPATNPGSGSRGGAGGSGGNGGAGGNATGSGGKGGAGGNGGDGSFGATSGPASIGVTGAPGGNGGKGGAGGSNPNGSGGDGGKGGNGGAGGNGGSIGANSGIVGGSGGAGGAGGAGGNGSLSSGEGGKGGDGGHGGDGVGGNSSVTQGGSGGGGGAGGAGGSGFFGGKGGFGGDGGQGGPNGGGTVGTVAGGGGNGGVGGRGGDGVFAGAGGQGGLGGQGGNGGGSTGGNGGLGGAGGGGGNAPDGGFGGNGGKGGQGGIGGGTQSATGLGGDGGDGGDGGNGGNSGAKAGGAGGKGQAGQPNSGTEPGFGGDGGLGGAGATP
Prediction	8625525635615515615516616526524527425236256165155166165166165144146145256155146155155246256246255255136165155245256255255146155256255165165155166145246256256166144246236356425426527424526526615615515616616514614515514615516616516515614616616616616514616515415645344364354454444363526527426426526426415455524524626626625525614515626525625614526616514524626626425524614526525626424614616516526524526616516427525517526426424433553562451651661651642642454255246166164256164145166146266168
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AQASPAAHGGSGGAGGNGGAGSAGNGGAGGAGGNGGAGGNGGGGDAGNAGSGGNGGKGGDGVGPGSTGGAGGKGGAGANGGSSNGNARGGNAGNGGHGGAGGSGDTGGAGGAGGQGGFGGTGGSGSGIGGGAGGNGGNGGAGGTGVVLGGKGGDGGNGDHGGPATNPGSGSRGGAGGSGGNGGAGGNATGSGGKGGAGGNGGDGSFGATSGPASIGVTGAPGGNGGKGGAGGSNPNGSGGDGGKGGNGGAGGNGGSIGANSGIVGGSGGAGGAGGAGGNGSLSSGEGGKGGDGGHGGDGVGGNSSVTQGGSGGGGGAGGAGGSGFFGGKGGFGGDGGQGGPNGGGTVGTVAGGGGNGGVGGRGGDGVFAGAGGQGGLGGQGGNGGGSTGGNGGLGGAGGGGGNAPDGGFGGNGGKGGQGGIGGGTQSATGLGGDGGDGGDGGNGGNSGAKAGGAGGKGQAGQPNSGTEPGFGGDGGLGGAGATP

1vt4I3

0.53

0.99

4.36

MUSTER

MAEDEAIFEEAHKQVQRGGGGGGGGGGGGGGGGGGGG--GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG

1vt4I3

0.53

0.99

7.16

dPPAS

MAEDEAIFEEAHKQVQRGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG

3hr2A

0.28

1.00

8.20

wdPPAS

-QMSYGYDEKSAGVSVPGPMGPSGPRGLGPGAGQGFQGPGEGEGGSGPMGPPGPPGPQGARGLGTAGLGMGHRGFSGLDGAKGDTGPAGPKGEGSGENGTGQMGPRGLGERGRGPGTAGARGNDGAVGAAGPGPTGPTGPGFGAAGAKGEAGPQGARGSEGPQGVRGEGPGPAGAADGQGAKGANGAGIAGAGFGARGPSGPQGPSGAGPKGTSGEGAGNKGDTGAKGEPGPAGEEGKRGARGEGPSGLGPGERGGGSRGFGADGVAGPKGPSGERGSGPAGPKGSGEAGRGEAGLGAKGLTGSGSGPDGKTGPGPAGQDGRGPAGPGARGQAGVMGFGPKGTAGEGKAGERGVGPGAVGPAGKDGEAGARGEQGPAGSGFQGLGPAGPGEAGKGEQGVGDLGAGPSGARGERGFGERGVQGPGPAGNDGAKGDTGAGAGSQGAGLQGMGERGAAGLGPKGDRGDAGPKGADGSPGKDGVRGLT

2pffA

0.52

1.00

8.27

wMUSTER

GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGVTFKDKYVLITGAKGSIGAEVLQGLLQGGAKV

1vt4I3

0.55

0.54

0.98

6.57

wPPAS

EAHKQVQRGGGGGGGGGGGGGGGGGGGGGG--GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GG--

1vt4I3

0.53

0.99

10.83

dPPAS2

DEAIFEEAHKQVQRGGGGGGGGGGGGGGGGG--GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG

1vt4I3

0.53

0.99

7.61

PPAS

MAEDEAIFEEAHKQVQRGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGG-GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG

3jacA1

0.54

0.39

0.71

3.65

Env-PPAS

--GGGGGGGGGGGGGGGGGGGGGGGGGGGGG-----GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG----------GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG------------------------------------GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-------------------------------------------------------------------------------------

3jacA1

0.55

0.39

0.71

3.48

MUSTER

--GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-----GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG----------GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG---------------------------------------GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG----------------------------------------------------------------------------------

3hr2A

0.25

1.00

6.37

dPPAS

LGTAGLGMGHRGFSGLDGAKGDTGPAGPKGEGSENGTGQMGPRGLGERGRGPGTAGARGNDGAVGAAGPGPTGPTGPGFGAAGAKGEAGPQGARGSEGPQGVRGEGPGPAGAAGPAGNGAQGAKGANGAGIAGAGFGARGPSGPQGPSGAGPKGTSGEGAGNKGDTGAKGEGPAGVQGPGPAGEEGKRGARGEGPSGLGPGERGGGSRGFGADGVAGPKGPSGERGSGPAGPKGSGEAGRGEAGLGAKGLTGSGSGPDGKTGPGPAGQDGRGPAGPGARGQAGVMGFGPKGTAGEGKAGERGVGPGAVGPAGKDGEAGAAGPAGPAGERGEQGPAGSGFQGLGPAGPGEAGKGEQGVGDLGAGPSGARGERGFGERGVQGPGPAGPRGNNGAGNDGAKGDTGAGAGSQGAGLQGMGERGAAGLGPKGDRGDAGPKGADGSPGKDGVRGLTGPIGPGPAGAGDKGEAGPSGPAGPTGARGAGDRG

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 2 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.50

Estimated TM-score = 0.92±0.06

Estimated RMSD = 4.1±2.8Å

Download Model 2

C-score=0.97

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1vt4I	0.935	2.35	0.528	0.986
2	3iytA	0.678	5.06	0.072	0.847
3	4nsxA	0.667	5.02	0.062	0.843
4	5hy7A	0.663	5.06	0.051	0.876
5	2ymuA	0.651	4.81	0.076	0.800
6	2oajA	0.641	5.47	0.057	0.884
7	5teeA	0.641	4.66	0.070	0.787
8	4lgnA	0.634	5.02	0.087	0.816
9	5fkqA	0.632	4.84	0.090	0.804
10	4xfvA	0.629	4.76	0.040	0.785

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.14	7	4gm8C	III	Rep, Mult	42,44,83,86,87,103,125,127,140,241,286,302
2	0.08	4	4j78A	III	Rep, Mult	83,87,103,127,157,158,160,176,197,199,214,240,261
3	0.02	1	1omw3	III	Rep, Mult	37,42,44,60,78,79,80,82,83,87,103,125,161,214,261
4	0.02	1	4a5tS	UUU	Rep, Mult	165,167
5	0.02	1	3ucpA	MG	Rep, Mult	257,260,263
6	0.02	1	1af0A	CA	Rep, Mult	319,320,321,323,330,331,332,335
7	0.02	1	1af0A	CA	Rep, Mult	360,361,362,363,365,389
8	0.02	1	1af0A	CA	Rep, Mult	310,311,312,321,322,323,326
9	0.02	1	3sbrF	IMD	Rep, Mult	214,215,239
10	0.02	1	2nzmA	CA	Rep, Mult	335,342
11	0.02	1	3odtA	CA	Rep, Mult	42,303,308
12	0.02	1	1tbg3	III	Rep, Mult	188,189,229,230
13	0.02	1	1af0A	CA	Rep, Mult	352,353,354,356,374,381
14	0.02	1	1o0tA	CA	Rep, Mult	229,230,231,233,243,245,248
15	0.02	1	3fbyA	CA	Rep, Mult	292,294,298,300,301,305
16	0.02	1	1go7P	CA	Rep, Mult	333,335,372,374

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2w68C	0.194	4.86	0.066	0.252	3.2.1.18	NA
2	0.060	1kv9A	0.500	4.17	0.089	0.612	1.1.99.-	241
3	0.060	1y9mA	0.436	5.50	0.056	0.591	3.2.1.80	NA
4	0.060	1e5tA	0.486	5.41	0.058	0.655	3.4.21.26	NA
5	0.060	1kb0A	0.487	4.85	0.077	0.618	1.1.99.-	NA
6	0.060	1kitA	0.433	4.46	0.045	0.541	3.2.1.18	NA
7	0.060	2h47H	0.438	3.20	0.069	0.500	1.4.99.4	166
8	0.060	1orvA	0.462	6.67	0.028	0.684	3.4.14.5	NA
9	0.060	2vk6A	0.434	4.70	0.074	0.554	3.2.1.18	207
10	0.060	3g0bB	0.461	6.46	0.025	0.678	3.4.14.5	214
11	0.060	2ecfA	0.486	6.77	0.054	0.740	3.4.14.5	305
12	0.060	2agsA	0.432	4.15	0.052	0.531	3.2.1.18	164,184,227
13	0.060	1w6sC	0.467	4.84	0.094	0.597	1.1.99.8	NA
14	0.060	1v0eA	0.440	4.79	0.066	0.558	3.2.1.129	NA
15	0.060	3c75J	0.378	3.58	0.034	0.444	1.4.99.3	336,350
16	0.060	1euuA	0.431	5.49	0.052	0.576	3.2.1.18	407
17	0.060	1lrwA	0.474	4.74	0.086	0.599	1.1.99.8	NA
18	0.060	1aomB	0.459	4.60	0.056	0.570	1.7.99.1,1.7.2.1	NA
19	0.060	2gbcA	0.466	6.65	0.028	0.690	3.4.14.5,3.4.15.5	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.23	0.651	4.81	0.08	0.80	2ymuA	GO:0016020 GO:0016021
1	0.12	0.619	4.63	0.05	0.77	3j2tA	GO:0000166 GO:0001666 GO:0001843 GO:0005524 GO:0005634 GO:0005737 GO:0005829 GO:0006915 GO:0006919 GO:0007275 GO:0007399 GO:0007420 GO:0007568 GO:0007584 GO:0008635 GO:0008656 GO:0010659 GO:0030154 GO:0030900 GO:0031072 GO:0034349 GO:0042802 GO:0042981 GO:0043065 GO:0043293 GO:0043531 GO:0051260 GO:0051402 GO:0070059 GO:0070062 GO:0071560 GO:0072432 GO:0097190 GO:0097193 GO:1902510 GO:2001235
2	0.11	0.619	4.86	0.07	0.77	1nr0A	GO:0003779 GO:0005737 GO:0005856 GO:0016528 GO:0030016 GO:0030240 GO:0030836 GO:0030837 GO:0040011 GO:0040012 GO:0042643 GO:0051015 GO:0071689
3	0.09	0.667	5.02	0.06	0.84	4nsxA	GO:0005634 GO:0005654 GO:0005730 GO:0006364 GO:0030529 GO:0031167 GO:0032040 GO:0034388 GO:0042254 GO:0042274
4	0.08	0.622	5.63	0.07	0.83	4ci8A	GO:0000226 GO:0002244 GO:0005509 GO:0005737 GO:0005829 GO:0005856 GO:0005874 GO:0005875 GO:0007052 GO:0007405 GO:0007420 GO:0008017 GO:0015631 GO:0048471 GO:0097431 GO:1990023
5	0.08	0.495	2.07	0.04	0.52	4yvdA	GO:0000398 GO:0000974 GO:0005634 GO:0005654 GO:0005662 GO:0005681 GO:0005730 GO:0006397 GO:0008380 GO:0016607 GO:0031965 GO:0034504 GO:0071011 GO:0071013 GO:0080008 GO:1900087
6	0.07	0.494	2.43	0.06	0.53	3odtA	GO:0005634 GO:0005737 GO:0006303 GO:0006511 GO:0010992 GO:0043130
7	0.07	0.503	2.03	0.06	0.53	3jcrL	GO:0000375 GO:0000398 GO:0005634 GO:0005654 GO:0005681 GO:0006396 GO:0006397 GO:0008380 GO:0015030 GO:0016607 GO:0017070 GO:0030621 GO:0046540 GO:0071001 GO:0097525
8	0.07	0.497	2.10	0.07	0.53	4lg8A	GO:0000209 GO:0000244 GO:0000245 GO:0000398 GO:0000974 GO:0001833 GO:0004842 GO:0005634 GO:0005654 GO:0005662 GO:0005681 GO:0005737 GO:0005811 GO:0005819 GO:0005856 GO:0006281 GO:0006283 GO:0006303 GO:0006397 GO:0006974 GO:0008380 GO:0008610 GO:0010498 GO:0016020 GO:0016567 GO:0016607 GO:0016874 GO:0034450 GO:0034613 GO:0035861 GO:0042802 GO:0045665 GO:0048026 GO:0048711 GO:0061630 GO:0070534 GO:0071013 GO:0072422
9	0.07	0.629	4.76	0.04	0.79	4xfvA	GO:0002098 GO:0005634 GO:0005654 GO:0005737 GO:0006351 GO:0006355 GO:0006357 GO:0006810 GO:0008017 GO:0015031 GO:0032447 GO:0033588
10	0.07	0.451	2.34	0.06	0.48	3ow8C	GO:0005634 GO:0005654 GO:0005737 GO:0006351 GO:0006355 GO:0016055 GO:0016593 GO:0032968 GO:0035327 GO:0045638 GO:0045944 GO:0051571 GO:0055087 GO:0080182 GO:2001162
11	0.07	0.614	5.16	0.06	0.82	3sfzA	GO:0000166 GO:0001666 GO:0001843 GO:0005524 GO:0005634 GO:0005737 GO:0005829 GO:0006915 GO:0006919 GO:0007275 GO:0007420 GO:0007568 GO:0007584 GO:0008635 GO:0008656 GO:0010659 GO:0030154 GO:0030900 GO:0031072 GO:0034349 GO:0042802 GO:0042981 GO:0043293 GO:0043531 GO:0051260 GO:0051402 GO:0070059 GO:0070062 GO:0071560 GO:0097190 GO:1902510 GO:2001235
12	0.07	0.604	4.68	0.07	0.75	1pguB	GO:0003779 GO:0005737 GO:0005856 GO:0005884 GO:0006970 GO:0030042 GO:0030479 GO:0032466 GO:0051016
13	0.07	0.497	2.16	0.06	0.53	3jb9K	GO:0000974 GO:0005634 GO:0005662 GO:0005681 GO:0006397 GO:0008380 GO:0045292 GO:0071011 GO:0071013 GO:0080008
14	0.07	0.499	2.10	0.06	0.53	5it9g	GO:0000747 GO:0001403 GO:0001965 GO:0004871 GO:0005080 GO:0005092 GO:0005737 GO:0007186 GO:0010255 GO:0017148 GO:0022627 GO:0031139 GO:0031954 GO:0032995 GO:0034613 GO:0035556 GO:0043022 GO:0050790 GO:0060733
15	0.07	0.481	2.17	0.05	0.51	2hesX	GO:0002098 GO:0005634 GO:0005737 GO:0005829 GO:0016226 GO:0044281 GO:0097361
16	0.07	0.457	2.91	0.07	0.51	3rfhA	GO:0001403 GO:0001965 GO:0004871 GO:0005092 GO:0005737 GO:0007186 GO:0010255 GO:0017148 GO:0022627 GO:0050790 GO:2001125
17	0.07	0.458	2.70	0.04	0.50	4jxmA	GO:0003723 GO:0005634 GO:0005654 GO:0005730 GO:0006364 GO:0030529 GO:0031428 GO:0032040 GO:0034511 GO:0044822
18	0.07	0.497	2.18	0.06	0.53	4v6iAa	GO:0001403 GO:0001965 GO:0004871 GO:0005092 GO:0005737 GO:0007186 GO:0010255 GO:0017148 GO:0022627 GO:0050790 GO:2001125
19	0.07	0.483	2.59	0.05	0.53	5a9q2	GO:0000775 GO:0000776 GO:0000777 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005694 GO:0005829 GO:0006406 GO:0006409 GO:0006810 GO:0007049 GO:0007059 GO:0007062 GO:0007067 GO:0007077 GO:0010827 GO:0015031 GO:0016032 GO:0016925 GO:0019083 GO:0031047 GO:0031080 GO:0051028 GO:0051301 GO:0075733 GO:1900034
20	0.07	0.454	2.41	0.03	0.49	5hqgA	GO:0000139 GO:0004842 GO:0005634 GO:0005654 GO:0005737 GO:0005813 GO:0005829 GO:0005925 GO:0008270 GO:0010212 GO:0016567 GO:0016607 GO:0016874 GO:0032436 GO:0046872 GO:0061630
21	0.07	0.465	2.01	0.06	0.49	5flcD	GO:0005654 GO:0005737 GO:0005829 GO:0007050 GO:0016236 GO:0030838 GO:0031295 GO:0031931 GO:0031932 GO:0032008 GO:0032956 GO:0043087 GO:0048015 GO:0050731 GO:1900034
22	0.07	0.488	2.84	0.05	0.54	4l9oA	GO:0005634 GO:0005643 GO:0005783 GO:0005789 GO:0006810 GO:0012507 GO:0015031 GO:0016020 GO:0016192 GO:0031410 GO:0051028
23	0.07	0.500	2.07	0.03	0.53	4btsAR	GO:0005840
24	0.07	0.489	3.37	0.04	0.56	4lg9A	GO:0000118 GO:0000122 GO:0002021 GO:0003677 GO:0003714 GO:0005634 GO:0005654 GO:0005876 GO:0006351 GO:0006355 GO:0008013 GO:0010468 GO:0016042 GO:0016568 GO:0016575 GO:0017053 GO:0030814 GO:0035264 GO:0042393 GO:0043161 GO:0044212 GO:0044255 GO:0045892 GO:0045893 GO:0045944 GO:0047485 GO:0050872 GO:0060070 GO:0060612 GO:0060613 GO:0090207
25	0.07	0.466	3.04	0.06	0.52	3mmyC	GO:0000972 GO:0003723 GO:0005634 GO:0005635 GO:0005643 GO:0005730 GO:0005737 GO:0006406 GO:0006409 GO:0006606 GO:0007077 GO:0008017 GO:0010827 GO:0016032 GO:0016925 GO:0019083 GO:0031047 GO:0043130 GO:0071407 GO:0075733 GO:1900034
26	0.07	0.497	2.54	0.06	0.54	5hynB	GO:0000122 GO:0001739 GO:0003682 GO:0005634 GO:0005654 GO:0005694 GO:0005730 GO:0006349 GO:0006351 GO:0006355 GO:0016568 GO:0016571 GO:0021510 GO:0035098 GO:0042054 GO:0042802 GO:0045120 GO:0045814 GO:0045892 GO:0061087
27	0.07	0.507	2.48	0.05	0.55	4a11B	GO:0000109 GO:0000209 GO:0003678 GO:0004842 GO:0005634 GO:0005654 GO:0006281 GO:0006283 GO:0006289 GO:0006974 GO:0006979 GO:0008094 GO:0009411 GO:0010165 GO:0016363 GO:0016567 GO:0031464 GO:0032403 GO:0043161 GO:0043234 GO:0045739 GO:0051865
28	0.07	0.496	2.23	0.03	0.53	5igoC	GO:0000152 GO:0004842 GO:0005634 GO:0005737 GO:0006281 GO:0008270 GO:0009585 GO:0009640 GO:0009641 GO:0009647 GO:0009649 GO:0010017 GO:0010119 GO:0016567 GO:0016604 GO:0016874 GO:0032436 GO:0042802 GO:0046283 GO:0046872 GO:0048573 GO:0061630 GO:0080008
29	0.07	0.504	2.12	0.05	0.54	3fm0A	GO:0006357 GO:0007059 GO:0008284 GO:0016226 GO:0071817 GO:0097361
30	0.07	0.495	2.19	0.07	0.53	2ce8A	GO:0000122 GO:0001106 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006351 GO:0006355 GO:0007165 GO:0007275 GO:0008134 GO:0009887 GO:0010628 GO:0016055 GO:0030178 GO:0042802 GO:0043124 GO:0045892 GO:1904837 GO:2000811
31	0.07	0.487	2.65	0.07	0.54	4u7aA	GO:0000463 GO:0000466 GO:0005634 GO:0005654 GO:0005730 GO:0006364 GO:0030687 GO:0042254 GO:0042273 GO:0043021 GO:0070180 GO:0070545
32	0.07	0.454	3.65	0.07	0.53	1r5mA	GO:0003714 GO:0005634 GO:0006355 GO:0006357 GO:0016568 GO:0016575 GO:0031939 GO:0032874 GO:0034967 GO:0045835 GO:1903507
33	0.07	0.500	2.59	0.07	0.54	4aezA	GO:0000070 GO:0005634 GO:0007049 GO:0007067 GO:0010997 GO:0032153 GO:0033597 GO:0034613 GO:0044732 GO:0051301 GO:0072686 GO:0097027 GO:1904668
34	0.07	0.501	2.84	0.06	0.55	4ui9R	GO:0005634 GO:0005654 GO:0005680 GO:0005737 GO:0005829 GO:0006281 GO:0006974 GO:0007049 GO:0007067 GO:0008284 GO:0010997 GO:0016567 GO:0031145 GO:0031572 GO:0031965 GO:0040020 GO:0042787 GO:0045732 GO:0051301 GO:0051436 GO:0051437 GO:0051439 GO:0070306 GO:0070979 GO:0090344 GO:0097027 GO:1904668
35	0.07	0.497	2.57	0.06	0.54	5emkB	GO:0000387 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005829 GO:0006357 GO:0008284 GO:0008285 GO:0008327 GO:0016274 GO:0030374 GO:0034709 GO:0035246 GO:0060528 GO:0060770
36	0.07	0.551	3.75	0.06	0.63	5cvlA	GO:0000724 GO:0005634 GO:0005737 GO:0005764 GO:0005768 GO:0005770 GO:0006974 GO:0007283 GO:0007338 GO:0008584 GO:0016032 GO:0016579 GO:0035264 GO:0043588 GO:0048568 GO:0048705 GO:0048872 GO:0050679 GO:0072520 GO:1902525
37	0.07	0.498	1.95	0.07	0.52	1b9xA	GO:0001750 GO:0003924 GO:0004871 GO:0005622 GO:0005765 GO:0005834 GO:0007165 GO:0007186 GO:0007191 GO:0007200 GO:0008283 GO:0016020 GO:0016056 GO:0022400 GO:0032403 GO:0043209 GO:0050909 GO:0051020 GO:0060041 GO:0070062 GO:0071380 GO:0071870 GO:0097381 GO:1903561
38	0.07	0.461	2.62	0.05	0.50	2h9lA	GO:0000123 GO:0001501 GO:0005622 GO:0005634 GO:0005654 GO:0005671 GO:0006351 GO:0006355 GO:0016568 GO:0018024 GO:0031175 GO:0035064 GO:0035097 GO:0035948 GO:0042800 GO:0043966 GO:0043981 GO:0043982 GO:0043984 GO:0043995 GO:0043996 GO:0044666 GO:0046972 GO:0048188 GO:0051568 GO:0071339
39	0.07	0.509	1.47	0.04	0.52	1vyhC	GO:0000132 GO:0000226 GO:0000235 GO:0000776 GO:0001667 GO:0001675 GO:0001764 GO:0001961 GO:0005634 GO:0005635 GO:0005737 GO:0005813 GO:0005815 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005938 GO:0006629 GO:0006810 GO:0007017 GO:0007049 GO:0007067 GO:0007097 GO:0007268 GO:0007275 GO:0007399 GO:0007405 GO:0007420 GO:0007611 GO:0008017 GO:0008090 GO:0008344 GO:0009306 GO:0010977 GO:0015630 GO:0016020 GO:0016042 GO:0016477 GO:0017145 GO:0019226 GO:0021540 GO:0021766 GO:0021819 GO:0021895 GO:0021987 GO:0030036 GO:0030154 GO:0030424 GO:0030426 GO:0031023 GO:0031252 GO:0031512 GO:0031965 GO:0031982 GO:0032403 GO:0032420 GO:0036035 GO:0040019 GO:0042249 GO:0042803 GO:0043005 GO:0043025 GO:0043087 GO:0043622 GO:0045502 GO:0045505 GO:0045773 GO:0045931 GO:0046329 GO:0047496 GO:0048471 GO:0048854 GO:0050885 GO:0051081 GO:0051130 GO:0051219 GO:0051301 GO:0051660 GO:0051661 GO:0060117 GO:0061003 GO:0070062 GO:0070507 GO:0090102 GO:0090176 GO:1904115 GO:2000574
40	0.07	0.504	1.88	0.07	0.53	3jcrD	GO:0000375 GO:0000398 GO:0005634 GO:0005654 GO:0005681 GO:0005682 GO:0005732 GO:0005737 GO:0006396 GO:0006397 GO:0008380 GO:0044822 GO:0071011 GO:0071013
41	0.07	0.455	2.87	0.04	0.50	3i2nA	GO:0006915 GO:0043130
42	0.07	0.445	2.11	0.03	0.47	3jb9L	GO:0005634 GO:0005681 GO:0005682 GO:0006397 GO:0008380 GO:0045292
43	0.07	0.457	2.67	0.04	0.50	1erjC	GO:0000122 GO:0000123 GO:0000433 GO:0001191 GO:0001198 GO:0005634 GO:0006351 GO:0006355 GO:0007070 GO:0008023 GO:0016584 GO:0017053 GO:0033588 GO:0035955 GO:0036033 GO:0042393 GO:0042826 GO:0043486 GO:0080025 GO:2000217 GO:2000531 GO:2001020
44	0.07	0.497	1.94	0.07	0.52	2pbiD	GO:0001750 GO:0001917 GO:0004871 GO:0005096 GO:0005634 GO:0005829 GO:0005886 GO:0007165 GO:0007186 GO:0016020 GO:0031682 GO:0043209 GO:0043547 GO:0051087 GO:1901386
45	0.07	0.498	2.05	0.03	0.53	4j87A	GO:0000139 GO:0005198 GO:0005737 GO:0005794 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0015031 GO:0016020 GO:0016192 GO:0030117 GO:0030126 GO:0030137
46	0.07	0.503	2.17	0.06	0.54	4v6wAg	GO:0000398 GO:0005080 GO:0005737 GO:0005776 GO:0005829 GO:0005875 GO:0007626 GO:0009267 GO:0016243 GO:0018991 GO:0035220 GO:0042335 GO:0044829 GO:0045725 GO:0046716 GO:0048477 GO:0071011 GO:0071013 GO:0075522
47	0.07	0.429	2.40	0.06	0.46	4g56D	GO:0005634 GO:0005737 GO:0005829 GO:0034709 GO:0035097

Consensus prediction of GO terms

Molecular Function	GO:0005524	GO:0008656	GO:0042802	GO:0043531	GO:0031072	GO:0051015	GO:0005509	GO:0008017
GO-Score	0.12	0.12	0.12	0.12	0.12	0.11	0.08	0.08
Biological Processes	GO:0048513	GO:0007417	GO:0060322
GO-Score	0.38	0.38	0.38
Cellular Component	GO:0043231	GO:0044444	GO:0031224	GO:0043232
GO-Score	0.40	0.38	0.36	0.36

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.