I-TASSER results

I-TASSER results for job id Rv3254

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (462 residues)
MVIGASIAGLCAARVLSDFYSTVTVFERDELPEAPANRATVPQDRHLHMLMARGAQEFDS
LFPGLLHDMVAAGVPMLENRPDCIYLGAAGHVLGTGHTLRKEFTAYVPSRPHLEWQLRRR
VLQLSNVQIVRRLVTEPQFERRQQRVVGVLLDSPGSGQDREREEFIAADLVVDAAGRGTR
LPVWLTQWGYRRPAEDTVDIGISYASHQFRIPDGLIAEKVVVAGASHDQSLGLGMLCYED
GTWVLTTFGVADAKPPPTFDEMRALADKLLPARFTAALAQAQPIGCPAFHAFPASRWRRY
DKLERFPRGIVPFGDAVASFNPTFGQGMTMTSLQAGHLRRALKARNSAMKGDLAAELNRA
TAKTTYPVWMMNAIGDISFHHATAEPLPRWWRPAGSLFDQFLGAAETDPVLAEWFLRRFS
LLDSLYMVPSVPIIGRAIAHNLRLWLKEQRERRQPVTTRRSP

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MVIGASIAGLCAARVLSDFYSTVTVFERDELPEAPANRATVPQDRHLHMLMARGAQEFDSLFPGLLHDMVAAGVPMLENRPDCIYLGAAGHVLGTGHTLRKEFTAYVPSRPHLEWQLRRRVLQLSNVQIVRRLVTEPQFERRQQRVVGVLLDSPGSGQDREREEFIAADLVVDAAGRGTRLPVWLTQWGYRRPAEDTVDIGISYASHQFRIPDGLIAEKVVVAGASHDQSLGLGMLCYEDGTWVLTTFGVADAKPPPTFDEMRALADKLLPARFTAALAQAQPIGCPAFHAFPASRWRRYDKLERFPRGIVPFGDAVASFNPTFGQGMTMTSLQAGHLRRALKARNSAMKGDLAAELNRATAKTTYPVWMMNAIGDISFHHATAEPLPRWWRPAGSLFDQFLGAAETDPVLAEWFLRRFSLLDSLYMVPSVPIIGRAIAHNLRLWLKEQRERRQPVTTRRSP
Prediction	CEEHCCHHHHHHHHHHHHHCCEEEEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEHCCCCCEEEEHCCCEHCCCCCCCCCCCEEEHCCCHHHHHHHHHHHHHCCCEEEEHCEEEHCEHCCCCCEEEEEEEHCCCCCCCCCCCEEEHCCEEEHCCCCCCCCHHHHHHHCCCCCCCEEEEEEEEEEEEEEHCCCCCCCCEEEEHCCCCCCCCEEEEEEHCCCEEEEEEHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
Conf.Score	856465899999999876578799997789999988899999754677888999999999889878999988986766789779998695766778888888679977876679999999998999799757566645658998677999985888888887579876567768997777879999989999997899985788888787899988775999868999984899998659989999864679999999799999999869889999999798889864578887644444567779998687544466658887767889999999999999859998887667999999855556656666545567888889999856778999999999997558999999999985778856578968999999997566777777777888888999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MVIGASIAGLCAARVLSDFYSTVTVFERDELPEAPANRATVPQDRHLHMLMARGAQEFDSLFPGLLHDMVAAGVPMLENRPDCIYLGAAGHVLGTGHTLRKEFTAYVPSRPHLEWQLRRRVLQLSNVQIVRRLVTEPQFERRQQRVVGVLLDSPGSGQDREREEFIAADLVVDAAGRGTRLPVWLTQWGYRRPAEDTVDIGISYASHQFRIPDGLIAEKVVVAGASHDQSLGLGMLCYEDGTWVLTTFGVADAKPPPTFDEMRALADKLLPARFTAALAQAQPIGCPAFHAFPASRWRRYDKLERFPRGIVPFGDAVASFNPTFGQGMTMTSLQAGHLRRALKARNSAMKGDLAAELNRATAKTTYPVWMMNAIGDISFHHATAEPLPRWWRPAGSLFDQFLGAAETDPVLAEWFLRRFSLLDSLYMVPSVPIIGRAIAHNLRLWLKEQRERRQPVTTRRSP
Prediction	300001100000000001114300000004027534303000001000000310110013002201200141201101034000000000110344441333010000000000210020027144020111011112124532000000012334355454331030000000010000003002614163242340401000000101023611322000000014000000000004100000000114231022261013005600122012003602211312402110010000220622040000100000000020001000000002101300443564434300210021004103203300010112214142523221100000003100400430030020002003002203101100000000000123335644535544645658
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CEEHCCHHHHHHHHHHHHHCCEEEEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEHCCCCCEEEEHCCCEHCCCCCCCCCCCEEEHCCCHHHHHHHHHHHHHCCCEEEEHCEEEHCEHCCCCCEEEEEEEHCCCCCCCCCCCEEEHCCEEEHCCCCCCCCHHHHHHHCCCCCCCEEEEEEEEEEEEEEHCCCCCCCCEEEEHCCCCCCCCEEEEEEHCCCEEEEEEHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
MVIGASIAGLCAARVLSDFYSTVTVFERDELPEAPANRATVPQDRHLHMLMARGAQEFDSLFPGLLHDMVAAGVPMLENRPDCIYLGAAGHVLGTGHTLRKEFTAYVPSRPHLEWQLRRRVLQLSNVQIVRRLVTEPQFERRQQRVVGVLLDSPGSGQDREREEFIAADLVVDAAGRGTRLPVWLTQWGYRRPAEDTVDIGISYASHQFRIPDGLIAEKVVVAGASHDQSLGLGMLCYEDGTWVLTTFGVADAKPPPTFDEMRALADKLLPARFTAALAQAQPIGCPAFHAFPASRWRRYDKLERFPRGIVPFGDAVASFNPTFGQGMTMTSLQAGHLRRALKARNSAMKGDLAAELNRATAKTTYPVWMMNAIGDISFHHATAEPLPRWWRPAGSLFDQFLGAAETDPVLAEWFLRRFSLLDSLYMVPSVPIIGRAIAHNLRLWLKEQRERRQPVTTRRSP

3e1tA

0.20

0.18

0.86

1.36

MUSTER

IVIGGGPGGSTLASFVAM-HR-VLLLEREAFP-RHQIGESL--------LPATVHGICAML--GLTDEMKRAGFPIK--RGGTFRWGKEPTFGFTRHPDDPYGFAYQVERARFDDMLLRNSERK-GVDVREREVIDVLFEGE--RAVGVRYRNTEG-----VELMAHARFIVDASGNRTRVSQAV---GERYSRFFQNVALYGYFENGKRLPAPRQGN--ILSAAFQD---GWFWYIPSDTLTSVGAVVSREAAEAGHEAALLRYIDRC--PIIKEYLAPATRVGDYGEIRIRKDYSYCNTSFWK--NGMALVGDAACFVDPVFSSGVHLATYSALLVARAINT---CLAGE---------MSEQRCFEEFER---------------RYRREYGNFYQFLVAFYDMNQDTDSYFWSARKIINTEERA--NEAFVRLIAG--RSNLDEMQGFTRMFGLVPSR

3e1tA

0.17

0.15

0.85

3.77

dPPAS

IVIGGGPGGSTLASFVAMRGHRVLLLEREAFPRHQIG---------ESLLPATVHGICAML--GLTDEMKRAGFPIK----RGGTFRWGKTFGFTRHPDDPYGFAYQVERARFDDMLLRNSERKGVDVRERHEVIDVLFEGE--RAVGVRYRNTEG-----VELMAHARFIVDASGNRTRVSQAV---GERVYSRFFQNVALYGYFENGKRLPAPRQGNILSAAFQDG---WFWYIPLSDTLTSVGAVVAAEAIKDGHEAALLRYIDRC--PIIKEYLAPATRVGDYGEIRIRKDYSYCNTSFWK--NGMALVGDAACFVDPVFSSGVHLATYSALLVARAINTCLAGEMS--------------------------------------EQRCFEEFERRYRREYGNFYQFLVAFYDMNQDTDSYFWSNTEERANEAFVRLIAGRSNLDEPVFQSNFMQGFT

3e1tA

0.20

0.17

0.85

3.71

wdPPAS

IVIGGGPGGSTLASFVAM---RVLLLEREAFPRHQI------------LLPATVHGICAML--GLTDEMKRAGFPIKRG---GTFRWGKEWTFGFTRHPDDPYFAYQVERARFDDMLLRNSERK-GVDVREREVIDVLFE--GERAVGVRYRNTEG-----VELMAHARFIVDASGNRTRVSQAV---GERVYSR-QNVALYGYFENGKRLPAPRQGNILSAAFQD-----GWFWYIPSDTLTSVGAV--AEAIKDGHEAALLRYIDRC--PIIKEYLAPATRVTTGDYGEIDYSY------NTSF-NGMALVGDAACFVDPVFSSGVHLATYSALLVARAINT-GEMSEQRCFEEFERRYRREYGNFYQFLV----AFYDMNQDTDSYFWSARKII--------NTEERANEAFVRLIA-LDEPVFQSNF-REITELQHLAMFGLVPSRDGLAWA------

3e1tA

0.21

0.18

0.87

1.40

wMUSTER

IVIGGGPGGSTLASFVAM-HR-VLLLEREAFP-----RHQI------SLLPATVHGICAML--GLTDEMKRAGFPIKRGG--TFRWGKEPEPWFTRHPDDPYGFAYQVERARFDDMLLRNSERK-GVDVREREVIDVLFEGE--RAVGVRYRNT-EG----VELMAHARFIVDASGNRTRVSQAV---GERYSRFFQNVALYGYFENGKRLPAPRQGN--ILSAAFQD---GWFWYIPSDTLTSVGAVVAAEAIKDGHEAALLRYIDRC--PIIKEYLAPATRVGDYGEIRIRKDYSYCNTSFWK--NGMALVGDAACFVDPVFSSGVHLATYSALLVARAINT-GEMSEQRCFEEFERRYRREYGNFYQFLV----AFYDMNQDTDSYFWSARKII--------NTEERANEAFVRLIA-LDEPVFQSNF----------MQGFTREELQHLAMFGLVPSR

3e1tA

0.20

0.18

0.86

1.59

wPPAS

IVIGGGPGGSTLASFVAM---RVLLLEREAFP-----RHQI------SLLPATVHGICAML--GLTDEMKRAGFPIKRG---GTFRWGKEWTFGFTRHPDDPYFAYQVERARFDDMLLRNSER-KGVDVREREVIDVLFEGE--RAVGVRYRNTEG-----VELMAHARFIVDASGNRTRVSQAV---GERVYSRFQNVALYGYFENGKRLPAPRQGN--ILSAAFQD---GWFWYIPSDTLTSVGAVVSREAAEAIKEAALLRYIDRC--PIIKEYLAPATRVTTGDYGEIRIDYSYCNTSFWK--NGMALVGDAACFVDPVFSSGVHLATYSALLVARAINT-GEMSEQRCFEEFERRYRREYGNFYQFLV----AFYDMNQDT--------DSYFWSARKIINTEERANEAFVRLIA-LDEPVFQSNF--FTREITELQHL---------AMFGLVPSR

3e1tA

0.16

0.13

0.83

3.79

dPPAS2

IVIGGGPGGSTLASFVAMRGHRVLLLEREAFPRHQIG---------ESLLPATVHGICAML--GLTDEMKRAGFPIK----RGGTFRWGKWTFGFTRHPDDPYFAYQVERARFDDMLLRNSERK-GVDVRERHEVIDVLFEG-ERAVGVRYRNTEG-----VELMAHARFIVDASGNRTRVSQAV---GERVYSRFFQNVALYGYFENGKRLPAPRQGNILSAAFQDG---WFWYIPLSDTLTSVGAVVAIKDGH---EAALLRYIDRC--PIIKEYLAPATRVTTGDYYSYCNTSFWK--------NGMALVGDAACFVDPVFSSGVHLATYSALLVARAINTCLAGEMS--------------------------------------EQRCFEEFERRYRREYGNFYQFLVAFYDMNQDTDSYFWSAREERANEAFVRLIAGRSNLDEPVFQSNFMQGFT

3e1tA

0.17

0.15

0.88

1.77

PPAS

IVIGGGPGGSTLASFVAMRGHRVLLLEREAFPRHQ--IGE-------SLLPATVHGICAML--GLTDEMKRAGFPIKR----GGTFRWGKWTFGFTRHPDDPYFAYQVERARFDDMLLRNSER-KGVDVRERHEVI-DVLFEGERAVGVRYRNTEG-----VELMAHARFIVDASGNRTRVSQAV---GERVYSRFQNVALYGYFENGKRLPAPRQGNILSAAF-----QDGWFWYIPSDTLTSVGAVVSREAAEAIKEAALLRYIDRC--PIIKEYLAPATRVGDYGEIRIRKDYSYCNTSFWK--NGMALVGDAACFVDPVFSSGVHLATYSALLVARAINTCLAG--EMSEQRCFEEFERRYRREYGNFYQFLVAFYDMNQDTDS--------YFWSARKIINTEERANEAFVRLIA-LDEPVFQSNFGFTREITELQHL----------AMFGLVPSR

3e1tA

0.18

0.16

0.90

2.70

Env-PPAS

IVIGGGPGGSTLASFVAMRGHRVLLLEREAFPRHQ--IGE-------SLLPATVHGICAML--GLTDEMKRAGFPIKRGG--TFRWGKEPWTFGFTRHPDDPYFAYQVERARFDDMLLRNSERK-GVDVRERHEVI-DVLFEGERAVGVRYRNTEG-----VELMAHARFIVDASGNRTRVSQAV---GERVYSRFQNVALYGYFENGKRLPAPRQGNILSAAFQ----DGWFWYIPLSDTLTSVGAVVSREAAEAIKEAALLRYIDRC--PIIKEYLAPATRVGDYGEIRIRKDYSYCNTSFWK--NGMALVGDAACFVDPVFSSGVHLATYSALLVARAINTCLAGE--MSEQRCFEEFERRYRREYGNFYQFLVAFYDMNQDTDS--------YFWSARKIINTEERANEAFVRLIANLDEPVFQSNFQGFTREITELQHLAMFGLVPSRDGLAWA---

3i3lA1

0.16

0.14

0.85

1.31

MUSTER

AIIGGGPAGSVAGLTLHKL--DVTIYERSAFP--RYRVGES--------LLPGTMSILNRL--GLQEKIDAQNYVKKPSA--TFLWGQAPWTFSFAAPAPWVFHAVQVKREEFDKLLLDEARSR-GITVHEETVTDVDLSDPDR--VVLTVRRGG------ESVTVESDFVIDAGGSGG---PISRKLGVRQYDEFYRNFAVWSYFKLKDPFEGDLKGTTYSITFED----GWVWMIP-DDLYSVGLVVDRSKSAEVREQGADAFYSSTLCAKAMDILGGAEQVDEVRIWSYDTEVFSA--------DRFFLCGDAACFTDPLFSQGVHLASQSAVSAAAAIDRIHGDEKDAVHAWYNRTYREAYEQYHQFLA---------------SFYTFASFTEPEFWRKRRITESDDDRLTRKKW-FESF-----RDRASTMIAIGR-HQRPELSDDFSEAEL----

3i3lA1

0.13

0.11

0.86

3.59

dPPAS

AIIGGGPAGSVAGLTLHKLGHDVTIYERSAFPRYRVGE----------SLLPGTMSILNRL--GLQEKIDAQNYVKK----PSATFLWGQTFSFAAPAPWVFDHAVQVKREEFDKLLLDEARSR-GITVHEETPVTDVDLSDPDRVVLTVRR-------GGESVTVESDFVIDAGGSGG---PISRKLGVRQYDEFYRNFAVWSYFKLKDPFEGDLKGTTYSITFEDG---WVWMIPIKDDLYSVGLVVDRSKSAEVREQGADAFYSSTKCAKAMDILGGAEQVDEVRIWSYDTEVFSA--------DRFFLCGDAACFTDPLFSQGVHLASQSAVSAAAAIDRITRH--GDEKDAVHAWYNRTYREAYEQYHQ--------------FLASFYTFASFEFWRKRRITESDDDRLTRKKWFES-----FRDRASTMIAIGRHQRPELSDDFSEAEL------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.18

Estimated TM-score = 0.57±0.15

Estimated RMSD = 9.9±4.6Å

Download Model 2

C-score=-2.37

Download Model 3

C-score=-2.74

Download Model 4

C-score=-2.71

Download Model 5

C-score=-3.84

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3e1tA	0.794	2.94	0.159	0.870
2	3i3lA	0.753	3.39	0.125	0.853
3	5dbjE	0.714	2.99	0.113	0.788
4	5uaoA	0.702	3.77	0.140	0.823
5	3ihgA	0.702	3.91	0.129	0.838
6	5hy5A	0.699	3.34	0.123	0.805
7	2wetB	0.696	3.32	0.119	0.801
8	2pyxA	0.691	3.65	0.091	0.812
9	2oalB	0.688	3.76	0.121	0.809
10	3atqA	0.688	4.48	0.091	0.842

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.58	166	2qa1A	FAD	Rep, Mult	3,4,6,7,8,26,27,28,29,45,110,133,134,135,174,175,176,180,202,293,314,315,322,325,326,327,328,329,331
2	0.00	1	5kxjA	ASP	Rep, Mult	8,314,315,327,328,331
3	0.00	1	2oa1B	ADN	Rep, Mult	4,5,28,132,133,134,174,175,180

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.271	1dodA	0.661	4.23	0.094	0.801	1.14.13.2	3,28,176,296,315
2	0.174	1ng4B	0.537	4.26	0.109	0.658	1.4.3.19	4,27,40
3	0.088	1c0kA	0.507	4.33	0.110	0.623	1.4.3.3	4,298,319
4	0.067	2uzzB	0.540	4.23	0.070	0.660	1.5.3.-	45
5	0.067	1aa8B	0.503	4.29	0.113	0.617	1.4.3.3	4,40,201,248
6	0.067	1vrqB	0.556	4.41	0.109	0.682	1.5.3.1	NA
7	0.067	2du8A	0.502	4.32	0.131	0.615	1.4.3.3	NA
8	0.067	1x31B	0.556	4.38	0.109	0.682	1.5.3.1	244
9	0.066	1c0iA	0.508	4.31	0.110	0.623	1.4.3.3	NA
10	0.060	3cirM	0.506	4.60	0.101	0.632	1.3.99.1	263
11	0.060	1zovA	0.547	4.13	0.101	0.664	1.5.3.1	45
12	0.060	1ng3A	0.536	4.22	0.112	0.656	1.4.3.19,1.5.3.-	NA
13	0.060	2wdqA	0.547	5.27	0.110	0.717	1.3.99.1	4,8,176
14	0.060	2z5xA	0.549	5.12	0.087	0.714	1.4.3.4	NA
15	0.060	3cp8A	0.577	4.66	0.082	0.725	1.-.-.-	NA
16	0.060	2dkiA	0.674	4.06	0.120	0.814	1.14.13.23	NA
17	0.060	1nekA	0.542	5.26	0.110	0.706	1.3.99.1,	NA
18	0.060	1knpA	0.528	5.37	0.115	0.688	1.4.3.16	NA
19	0.060	1d7lA	0.659	4.30	0.099	0.809	1.14.13.2	NA
20	0.060	2jb2A	0.525	4.54	0.129	0.660	1.4.3.2	4,294,319
21	0.060	1o5wC	0.544	5.28	0.090	0.717	1.4.3.4	4
22	0.060	2gmhA	0.671	4.05	0.124	0.805	1.5.5.1	4,8,40,112,176
23	0.060	1fohC	0.678	4.50	0.128	0.842	1.14.13.7	NA
24	0.060	1yq3A	0.550	5.38	0.085	0.732	1.3.5.1	NA
25	0.060	3cirA	0.526	5.14	0.099	0.682	1.3.99.1	308
26	0.060	2yr4A	0.540	4.66	0.080	0.693	1.13.12.9	4
27	0.060	1y56B	0.553	3.78	0.103	0.654	1.5.99.8	NA
28	0.060	1f8rA	0.523	4.62	0.130	0.662	1.4.3.2	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.31	0.709	3.09	0.11	0.79	5bukA	GO:0016491 GO:0055114
1	0.31	0.753	3.39	0.12	0.85	3i3lA	GO:0000166 GO:0016491 GO:0055114
2	0.31	0.794	2.94	0.16	0.87	3e1tA
3	0.29	0.699	3.34	0.12	0.81	5hy5A	GO:0000166 GO:0016491 GO:0055114
4	0.28	0.699	3.68	0.09	0.82	2pyxA	GO:0000166 GO:0016491 GO:0055114
5	0.28	0.675	4.29	0.10	0.82	4z2rA	GO:0004497 GO:0016491 GO:0055114 GO:0071949
6	0.28	0.715	2.96	0.11	0.79	5dbjC	GO:0016491 GO:0016787 GO:0047651 GO:0055114
7	0.25	0.701	3.05	0.12	0.78	3nixA	GO:0000166 GO:0016491 GO:0055114 GO:0071949
8	0.25	0.619	3.78	0.14	0.74	4a6nA	GO:0000166 GO:0016491 GO:0055114 GO:0071949
9	0.25	0.686	3.74	0.12	0.81	2e4gA	GO:0000166 GO:0016491 GO:0055114
10	0.25	0.666	4.39	0.13	0.81	2qa1A	GO:0000166 GO:0016491 GO:0055114 GO:0071949
11	0.24	0.642	3.61	0.13	0.75	2x3nA	GO:0000166 GO:0004497 GO:0016491 GO:0044550 GO:0055114 GO:0071949
12	0.23	0.644	3.89	0.14	0.76	3allB	GO:0000166 GO:0016491 GO:0055114 GO:0071949
13	0.23	0.696	3.32	0.12	0.80	2wetB	GO:0000166 GO:0016491 GO:0055114
14	0.22	0.689	3.71	0.13	0.81	2apgA	GO:0000166 GO:0016491 GO:0017000 GO:0055114
15	0.22	0.675	3.77	0.11	0.79	3oz2A	GO:0005886 GO:0006629 GO:0006650 GO:0008654 GO:0016020 GO:0016491 GO:0016628 GO:0045550 GO:0046467 GO:0050660 GO:0050661 GO:0051287 GO:0055114 GO:0071949
16	0.19	0.628	4.12	0.11	0.76	3ihmA	GO:0004497 GO:0016491 GO:0055114
17	0.10	0.657	4.69	0.12	0.81	4k2xB	GO:0000166 GO:0016491 GO:0055114 GO:0071949
18	0.07	0.632	4.28	0.13	0.76	4icyA	GO:0000166 GO:0016491 GO:0055114 GO:0071949
19	0.07	0.633	4.30	0.10	0.77	4cy8A	GO:0004497 GO:0016491 GO:0055114 GO:0071949

Consensus prediction of GO terms

Molecular Function	GO:0016491	GO:0000166
GO-Score	0.76	0.65
Biological Processes	GO:0055114
GO-Score	0.76
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.