I-TASSER results

I-TASSER results for job id Rv3231c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (169 residues)
MTQVYIPATLAMLQRLVADGALWPVNGTAFAVTPTLRESYAEGDDEELAEVALREAALAS
LRLLAADIGATADALPPRRAVLAAEVDDATYRPDLDDAVVRLAGPITIDQVVAAYVDNAG
AEPAVMAAIAVIDAADLGDEDAELVVGDAQDHDLAWYANQELPFLLDLL

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 \| \| \| \| \| \| \| \| MTQVYIPATLAMLQRLVADGALWPVNGTAFAVTPTLRESYAEGDDEELAEVALREAALASLRLLAADIGATADALPPRRAVLAAEVDDATYRPDLDDAVVRLAGPITIDQVVAAYVDNAGAEPAVMAAIAVIDAADLGDEDAELVVGDAQDHDLAWYANQELPFLLDLL
Prediction	CEEEEEHCCHHHHHHHHHHCCEHCCCCEEEEHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHCCCCCCCCCCCCCEEEHCCCCCHHHEEEEHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHC
Conf.Score	9699986788999999876745467766887588888876788878999999999999999999858787766888758999987588778888888689985789966657777487568999999999988745789678988765556444444657899999869
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 \| \| \| \| \| \| \| \| MTQVYIPATLAMLQRLVADGALWPVNGTAFAVTPTLRESYAEGDDEELAEVALREAALASLRLLAADIGATADALPPRRAVLAAEVDDATYRPDLDDAVVRLAGPITIDQVVAAYVDNAGAEPAVMAAIAVIDAADLGDEDAELVVGDAQDHDLAWYANQELPFLLDLL
Prediction	6100000110410441266351434412010001103522474343112320143003200311243464666522210000003055442566344221403450416300001023761462044015115515333630431154045330101125114302735
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160

                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CEEEEEHCCHHHHHHHHHHCCEHCCCCEEEEHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHCCCCCCCCCCCCCEEEHCCCCCHHHEEEEHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHC
MTQVYIPATLAMLQRLVADGALWPVNGTAFAVTPTLRESYAEGDDEELAEVALREAALASLRLLAADIGATADALPPRRAVLAAEVDDATYRPDLDDAVVRLAGPITIDQVVAAYVDNAGAEPAVMAAIAVIDAADLGDEDAELVVGDAQDHDLAWYANQELPFLLDLL

3iuvA

0.16

0.14

0.91

0.83

MUSTER

------PERIDAAIRVVGQKGIAGLSHRTVAVPLGSTTYH--ATLDDL-VAALRQANEGFARVVAAHPALSDPEADLSGEL--ARVLGEWLGGDRTGVELEYE----LYLAALRRPALRPVAAEWAEGVGALLAARTDPTTARALVAVLDGICLQVYDEEYAREVLTRL

3iuvA

0.14

0.12

0.92

0.77

dPPAS

------PERIDAAIRVVGQKGIAGLSHRTVAVPLGSTTYHF-ATLDDL-VAALRQANEGFARVVAAHPALSDPEADLSGELARVLGEWLGGDRTGVELEYE------LYLAALRRPALRPVAAEWAEGVGALLAARTDPTTARALVAVLDGICLQVYDEEYAREVLTRL

3iuvA

0.14

0.12

0.90

0.63

wdPPAS

------PERIDAAIRVVGQKGIAGLSHRTVAVPLGSTTYH--ATLDDL-VAALRQANEGFARVVAAHPALSDPEADLSGELARVLGEWLGGDRT------GVELEYELYLAALRRPALRPVAAEWAEGVGALLAARTDPTTARALVAVLD-QVLL-YDEEYAREVLTRL

3iuvA

0.16

0.14

0.91

0.82

wMUSTER

------PERIDAAIRVVGQKGIAGLSHRTVAVPGSTTYHF--ATLDDL-VAALRQANEGFARVVAAHPALSDPEADLSGEL--ARVLGEWLGGDRTGVELEYE----LYLAALRRPALRPVAAEWAEGVGALLAARTDPTTARALVAVLDGICLQVYDEEYAREVLTRL

3iuvA

0.13

0.11

0.85

0.65

wPPAS

IIRVIAGLSHRTVAAEA--------------VPLGSTTYH--ATLDDL-VAALRQANEGFARVVAAHPALSDPEADLSGELARVLGEWLGGDRTGVELEYEL-----YLA-ALRRPALRPVAAEWAEGVGALLAARTDPTTARALVAVLD-QVLL-YDEEYAREVLTRL

3iuvA

0.14

0.12

0.92

1.37

dPPAS2

------PERIDAAIRVVGQKGIAGLSHRTVAVPLGSTTYHF-ATLDDL-VAALRQANEGFARVVAAHPALSDPEADLSGELARVLGEWLGGDRTGVELEYE------LYLAALRRPALRPVAAEWAEGVGALLAARTDPTTARALVAVLDGICLQVYDEEYAREVLTRL

3iuvA

0.14

0.12

0.91

0.89

PPAS

------PERIDAAIRVVGQKGIAGLSHRTVAVPLGSTTYH--ATLDDL-VAALRQANEGFARVVAAHPALSDPEADLSGELARVLGEWLGGDRT------GVELEYELYLAALRRPALRPVAAEWAEGVGALLAARTDPTTARALVAVLDGICLQVYDEEYAREVLTRL

2omdB

0.13

0.11

0.82

0.69

Env-PPAS

IPRVYLGHEWFGAERILSEYQVPEDCGAQVLFLGIPRNAPEDGGNEALEYEAYPEMAIKEMEKIRQET---IEKFGVKEVFIHHRL-----------GLVKIGEPSFLVLAVGGHRE--ETFKACRYAVDETKK-RVPIWKKEIF----KEGKGEWVLGE---------

3bniA

0.13

0.12

0.92

0.76

MUSTER

LTRI-----LDACADLLDEVGYDALSTRAVAVPGSVYRFF--GNKRQMADALAQRNLERYAERVTERLTEAGDGGWRGALDTVLDEYLAMKRTAPGFSLIDFGRVAELTELLSGYLPDDDLRRVFLVAVETADTLRVAPDGDEKIIEEA--RELL---RAYLGRVLD--

5cwjA

0.11

0.10

0.91

0.63

dPPAS

-------DSEEEQERIRRILKEARKSGTEESLRQAIEDV-----AQLAKKSQDSEVLEEAIRVILRIAKESGSEEALRQAIRAV----AEIAKEAQDSEVLEEAIRVILRIAKESGSEEALRQAIRAVAEIAKEAQDPRVLEEAIRVIRQIAEESGSEEARRQAERAEE

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.04

Estimated TM-score = 0.58±0.14

Estimated RMSD = 7.2±4.2Å

Download Model 2

C-score=-2.04

Download Model 3

C-score=-5.00

Download Model 4

C-score=-5.00

Download Model 5

C-score=-3.57

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3iuvA	0.814	2.20	0.126	0.929
2	2dg8B	0.764	2.87	0.144	0.947
3	3e7qB	0.746	2.91	0.106	0.935
4	2g3bB	0.739	2.88	0.146	0.923
5	2np5A	0.735	2.79	0.149	0.911
6	2uxuA	0.728	2.87	0.063	0.917
7	5d1rA	0.724	3.26	0.132	0.929
8	3g7rB	0.717	3.21	0.138	0.935
9	3lsjA	0.715	2.98	0.140	0.905
10	1vi0A	0.715	2.88	0.118	0.905

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.13	9	3htjA	ET	Rep, Mult	57,60,81,84,85,88,99,100,125,149,150
2	0.07	5	3g1mA	RF3	Rep, Mult	64,81,82,85,126,132,133,136,146,149,150,153,166
3	0.04	3	5f04A	5TB	Rep, Mult	64,81,85,101,109,122,126,132,133,146,149,150,153,154,166
4	0.04	3	4f8hA	LMD	Rep, Mult	56,59
5	0.03	2	2eh30	III	Rep, Mult	101,109,112,113,118,122,125,140,143,144,146,147,148,150,151,154,155,156,161,164,165,168
6	0.03	2	1bjyB	CTC	Rep, Mult	162,166,169
7	0.03	2	1vi0B	DCC	Rep, Mult	147,150,151,154,155,158
8	0.01	1	2anuA	ZN	Rep, Mult	117,151
9	0.01	1	1mumA	MG	Rep, Mult	44,87
10	0.01	1	2dg80	III	Rep, Mult	17,18,21,23,102,109,110,112,113,119,122,123,126,141,144,146,147,150,151,152,155,165,168,169
11	0.01	1	2np5C	NDS	Rep, Mult	31,33,37
12	0.01	1	3g7rA	NA	Rep, Mult	40,41,44,47
13	0.01	1	3q2jA	CA	Rep, Mult	139,141,143
14	0.01	1	4evdA	CD	Rep, Mult	46,47,50
15	0.01	1	2zyqB	TAR	Rep, Mult	34,43
16	0.01	1	4ej7A	CA	Rep, Mult	85,87,161

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2jkvA	0.457	4.73	0.031	0.751	1.1.1.44	NA
2	0.060	3djlA	0.466	5.14	0.101	0.781	1.3.99.-	NA
3	0.060	2x38A	0.483	4.48	0.086	0.740	2.7.1.153	149
4	0.060	1v4tA	0.420	4.58	0.055	0.675	2.7.1.2,2.7.1.1	NA
5	0.060	2zidA	0.442	4.31	0.052	0.680	3.2.1.70	NA
6	0.060	1h16A	0.446	4.51	0.053	0.710	2.3.1.54	NA
7	0.060	3b4xA	0.459	4.10	0.083	0.657	1.14.14.1	123
8	0.060	3c6gB	0.447	4.06	0.051	0.651	1.14.13.15	48
9	0.060	2a4zA	0.502	4.33	0.090	0.757	2.7.1.153	125
10	0.060	1izjA	0.441	4.94	0.076	0.734	3.2.1.135,3.2.1.1	NA
11	0.060	1a31A	0.442	4.33	0.038	0.645	5.99.1.2	101
12	0.060	1hbmA	0.468	4.98	0.078	0.763	2.8.4.1	133
13	0.060	1rr8C	0.479	4.00	0.072	0.680	5.99.1.2	NA
14	0.060	1e6yA	0.476	4.84	0.059	0.763	2.8.4.1	NA
15	0.060	1ea9C	0.442	4.98	0.071	0.757	3.2.1.54	NA
16	0.060	2x6fA	0.476	4.26	0.085	0.716	2.7.1.137,2.7.1.153,2.7.1.154	61,64,123
17	0.060	1xmeA	0.442	4.85	0.075	0.728	1.9.3.1	NA
18	0.060	1p6xA	0.442	5.16	0.074	0.728	2.7.1.21	NA
19	0.060	1jqkF	0.428	4.99	0.068	0.722	1.2.99.2	123

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.30	0.715	3.12	0.11	0.93	4ichA	GO:0003677 GO:0006351 GO:0006355
1	0.29	0.728	3.20	0.14	0.95	3g7rB	GO:0003677 GO:0003700 GO:0006351 GO:0006355
2	0.29	0.769	2.63	0.12	0.94	3htaA	GO:0003677 GO:0006351 GO:0006355
3	0.29	0.672	3.25	0.13	0.91	3f1bA	GO:0003677 GO:0006351 GO:0006355
4	0.27	0.701	3.27	0.11	0.91	3on2B	GO:0003677 GO:0006351 GO:0006355
5	0.26	0.716	2.92	0.11	0.92	2id6A	GO:0003677 GO:0006351 GO:0006355
6	0.26	0.697	3.18	0.12	0.91	5d18A	GO:0003677 GO:0006351 GO:0006355
7	0.25	0.729	2.88	0.07	0.92	2uxhA	GO:0003677 GO:0006351 GO:0006355
8	0.24	0.718	3.15	0.14	0.94	3kkdC	GO:0003677 GO:0006351 GO:0006355
9	0.24	0.684	3.24	0.06	0.92	2f07A	GO:0003677 GO:0006351 GO:0006355
10	0.22	0.764	2.87	0.14	0.95	2dg8B	GO:0003677 GO:0006351 GO:0006355
11	0.22	0.634	3.35	0.12	0.89	3dewA	GO:0003677 GO:0006351 GO:0006355 GO:0016020 GO:0016021
12	0.22	0.709	3.49	0.10	0.95	3bcgA	GO:0003677 GO:0003700 GO:0006351 GO:0006355 GO:0008144 GO:0042493 GO:0043565 GO:0045892
13	0.22	0.612	3.51	0.10	0.86	3egqA	GO:0003677 GO:0006351 GO:0006355
14	0.21	0.746	2.91	0.11	0.93	3e7qB	GO:0003677 GO:0003700 GO:0006351 GO:0006355
15	0.20	0.691	3.36	0.18	0.92	3t6nA	GO:0003677 GO:0003700 GO:0006351 GO:0006355
16	0.20	0.689	3.75	0.10	0.95	3bruA	GO:0003677 GO:0006351 GO:0006355
17	0.19	0.615	3.54	0.13	0.85	3bqyA	GO:0003677 GO:0006351 GO:0006355 GO:0045892 GO:0046677
18	0.19	0.713	3.24	0.08	0.93	3p9tA	GO:0003677 GO:0006351 GO:0006355
19	0.19	0.632	3.64	0.06	0.89	3nnrA	GO:0003677 GO:0006351 GO:0006355
20	0.18	0.733	3.01	0.11	0.93	3whcA	GO:0003677 GO:0005737 GO:0006351 GO:0006355 GO:0006629 GO:0006631 GO:0016042
21	0.16	0.654	3.62	0.08	0.91	2zcnD	GO:0003677 GO:0006351 GO:0006355
22	0.16	0.668	3.55	0.07	0.92	3kz9B	GO:0003677 GO:0006351 GO:0006355
23	0.16	0.636	3.68	0.11	0.89	2rasB	GO:0003677 GO:0006351 GO:0006355
24	0.15	0.658	3.55	0.07	0.88	3vvxA	GO:0003677 GO:0006351 GO:0006355
25	0.14	0.639	3.60	0.10	0.88	2y31A	GO:0003677 GO:0006351 GO:0006355 GO:0045892
26	0.14	0.712	3.14	0.14	0.92	3ppbA	GO:0003677 GO:0006351 GO:0006355
27	0.13	0.709	3.38	0.09	0.94	3vibC	GO:0003677 GO:0006351 GO:0006355
28	0.13	0.721	3.30	0.15	0.95	2g3bA	GO:0003677 GO:0006351 GO:0006355
29	0.13	0.706	3.48	0.13	0.95	2hyjA	GO:0003677 GO:0003700 GO:0006351 GO:0006355
30	0.12	0.689	3.47	0.13	0.94	2nx4A	GO:0003677 GO:0006351 GO:0006355
31	0.12	0.682	3.02	0.11	0.89	2eh3A	GO:0003677 GO:0003700 GO:0006351 GO:0006355 GO:0046872
32	0.12	0.710	3.39	0.11	0.94	3lhqA	GO:0003677 GO:0003700 GO:0006351 GO:0006355
33	0.11	0.683	3.49	0.08	0.93	2pbxA
34	0.11	0.635	3.85	0.08	0.92	3geuA	GO:0003677 GO:0006351 GO:0006355
35	0.10	0.682	3.74	0.12	0.95	4l62A	GO:0000976 GO:0003677 GO:0003700 GO:0005829 GO:0006351 GO:0006355
36	0.09	0.655	3.25	0.12	0.85	3bniA	GO:0003677 GO:0006351 GO:0006355
37	0.09	0.649	3.28	0.06	0.86	2qkoB	GO:0003677 GO:0006351 GO:0006355
38	0.09	0.666	3.54	0.07	0.92	3on4A	GO:0003677 GO:0006351 GO:0006355
39	0.08	0.704	3.23	0.09	0.93	3lwjA	GO:0003677 GO:0006351 GO:0006355
40	0.08	0.669	3.17	0.12	0.91	3mvpA	GO:0003677 GO:0006351 GO:0006355
41	0.07	0.649	3.51	0.10	0.88	3cjdA	GO:0003677 GO:0006351 GO:0006355
42	0.07	0.654	3.76	0.11	0.89	3vuqC	GO:0003677 GO:0006351 GO:0006355
43	0.07	0.658	3.68	0.11	0.92	3s5rA	GO:0003677 GO:0006351 GO:0006355
44	0.07	0.649	3.57	0.14	0.91	3knwA	GO:0003677 GO:0006351 GO:0006355
45	0.07	0.633	3.95	0.11	0.90	4pxiB	GO:0003677 GO:0006351 GO:0006355
46	0.07	0.677	3.56	0.11	0.95	1t33A	GO:0003677 GO:0003700 GO:0006351 GO:0006355
47	0.07	0.622	3.67	0.07	0.86	3rh2A	GO:0003677 GO:0006351 GO:0006355
48	0.07	0.635	3.96	0.07	0.93	2fbqA	GO:0000976 GO:0003677 GO:0003700 GO:0005829 GO:0006351 GO:0006355 GO:0044212 GO:0045893 GO:0050714
49	0.07	0.624	3.74	0.07	0.87	3cdlB	GO:0003677 GO:0006351 GO:0006355
50	0.07	0.555	3.60	0.08	0.78	3ljlA	GO:0003677
51	0.07	0.614	3.47	0.07	0.85	4auxA	GO:0003677 GO:0006351 GO:0006355 GO:0045892 GO:0046677 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0003677	GO:0001071
GO-Score	0.81	0.58
Biological Processes	GO:0006355
GO-Score	0.81
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.