I-TASSER results

I-TASSER results for job id Rv3226c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (252 residues)
MCGRFAVTTDPAQLAEKITAIDEATGCGGGKTSYNVAPTDTIATVVSRHSEPDDEPTRRV
RLMRWGLIPSWIKAGPGGAPDAKGPPLINARADKVATSPAFRSAVRSKRCLVPMDGWYEW
RVDPDATPGRPNAKTPFFLHRHDGALLFTAGLWSVWKSYRSAPPLLSCTVITTDAVGELA
EIHDRMPLLLAEEDWDDWLNPDAPPDPELLARPPDVRDIALRQVSTLVNNVRNNGPELLE
PARSQPEQIQLL

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MCGRFAVTTDPAQLAEKITAIDEATGCGGGKTSYNVAPTDTIATVVSRHSEPDDEPTRRVRLMRWGLIPSWIKAGPGGAPDAKGPPLINARADKVATSPAFRSAVRSKRCLVPMDGWYEWRVDPDATPGRPNAKTPFFLHRHDGALLFTAGLWSVWKSYRSAPPLLSCTVITTDAVGELAEIHDRMPLLLAEEDWDDWLNPDAPPDPELLARPPDVRDIALRQVSTLVNNVRNNGPELLEPARSQPEQIQLL
Prediction	CCCEEEHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCCCCCCEEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCHCCCCCCCCCHHHHHHHHHCCEEEEEEEEEEEEHCCCCCCCCCCCCEEEEEHCCCCCEEEEEEHCCCCCCCCCCCEEEEEEEEHCCCCHHHHHHCCCCEEHCCHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEHCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
Conf.Score	998588669999999998786556777788988788999868999976888888765899999985687555467788877778887555567666667899998758789997567888757898888888765789875899779999863335688998578999999868976678765886676799999985589999889997579987877999857666887789978846567765556889
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MCGRFAVTTDPAQLAEKITAIDEATGCGGGKTSYNVAPTDTIATVVSRHSEPDDEPTRRVRLMRWGLIPSWIKAGPGGAPDAKGPPLINARADKVATSPAFRSAVRSKRCLVPMDGWYEWRVDPDATPGRPNAKTPFFLHRHDGALLFTAGLWSVWKSYRSAPPLLSCTVITTDAVGELAEIHDRMPLLLAEEDWDDWLNPDAPPDPELLARPPDVRDIALRQVSTLVNNVRNNGPELLEPARSQPEQIQLL
Prediction	853133024415301620542453664661322100214230000002445565424210210212023331443454446645230100101203234002300333100000000012244555454555423321222553200000000120343454330100010124236304502521000034720430143735523410331233430201301540442435244005305566564617
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCEEEHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCCCCCCEEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCHCCCCCCCCCHHHHHHHHHCCEEEEEEEEEEEEHCCCCCCCCCCCCEEEEEHCCCCCEEEEEEHCCCCCCCCCCCEEEEEEEEHCCCCHHHHHHCCCCEEHCCHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEHCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
MCGRFAVTTDPAQLAEKITAIDEATGCGGGKTSYNVAPTDTIATVVSRHSEPDDEPTRRVRLMRWGLIPSWIKAGPGGAPDAKGPPLINARADKVATSPAFRSAVRSKRCLVPMDGWYEWRVDPDATPGRPNAKTPFFLHRHDGALLFTAGLWSVWKSYRSAPPLLSCTVITTDAVGELAEIHDRMPLLLAEEDWDDWLNPDAPPDPELLARPPDVRDIALRQVSTLVNNVRNNGPELLEPARSQPEQIQLL

5ko9A

0.32

0.30

0.93

2.84

MUSTER

-CGRTSCHLPRDVLTRACAYQDRRGQQRLYCPSYNKSPQSNSPVLLSRFEKDADSSERIIAPMRWGLVPSWFKESD---PSKLQFNTTNCRSDTVMEKRSFKVPLGKRRCVVLADGFYEWQRCQG-----TNQRQPYFIYFPNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA---------

5ko9A

0.32

0.30

0.93

3.20

dPPAS

-CGRTSCHLPRDVLTRACAYQDRRGDPDKYCPSYNKSPQSNSPVLLSRFEKDADSSERIIAPMRWGLVPSWFKESDP---SKLQFNTTNCRSDTVMEKRSFKVPLGKRRCVVLADGFYEWQRCQG-----TNQRQPYFIYFDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA---------

5ko9A

0.32

0.30

0.93

5.01

wdPPAS

-CGRTSCHLPRDVLTRACAYQDRRGDPDKYCPSYNKSPQSNSPVLLSRFEKDADSSERIIAPMRWGLVPSWFKESDPS---KLQFNTTNCRSDTVMEKRSFKVPLGKRRCVVLADGFYEWQRCQG-----TNQRQPYFIYFDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA---------

5ko9A

0.32

0.30

0.92

3.52

wMUSTER

--GRTSCHLPRDVLTRACAYQDRRGDPDKYCPSYNKSPQSNSPVLLSRFEKDADSSERIIAPMRWGLVPSWFKESD---PSKLQFNTTNCRSDTVMEKRSFKVPLGKRRCVVLADGFYEWQRCQG-----TNQRQPYFIYFPNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA---------

5ko9A

0.32

0.30

0.93

5.64

wPPAS

-CGRTSCHLPRDVLTRACAYQDRRGDPDKYCPSYNKSPQSNSPVLLSRFEKDADSSERIIAPMRWGLVPSWFKESDP---SKLQFNTTNCRSDTVMEKRSFKVPLGKRRCVVLADGFYEWQRCQG-----TNQRQPYFIYFDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA---------

5ko9A

0.32

0.30

0.93

7.87

dPPAS2

-CGRTSCHLPRDVLTRACAYQDRRGDPDKYCPSYNKSPQSNSPVLLSRFEKDADSSERIIAPMRWGLVPSWFKESDP---SKLQFNTTNCRSDTVMEKRSFKVPLGKRRCVVLADGFYEWQRCQG-----TNQRQPYFIYFDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA---------

5ko9A

0.32

0.30

0.93

4.37

PPAS

-CGRTSCHLPRDVLTRACAYQDRRGDPDKYCPSYNKSPQSNSPVLLSRFEKDADSSERIIAPMRWGLVPSWFKESDP---SKLQFNTTNCRSDTVMEKRSFKVPLGKRRCVVLADGFYEWQRCQG-----TNQRQPYFIYFPNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA---------

5ko9A

0.32

0.30

0.93

5.78

Env-PPAS

-CGRTSCHLPRDVLTRACAYQDRRRDPDKYCPSYNKSPQSNSPVLLSRFEKDADSSERIIAPMRWGLVPSWFKES---DPSKLQFNTTNCRSDTVMEKRSFKVPLGKRRCVVLADGFYEWQRCQG-----TNQRQPYFIYFDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILDEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA---------

2bdvA

0.26

0.21

0.83

2.62

MUSTER

-CGRIAQKSAPEDYVEILWP----NARLVAGPRYNIPPGTRPLTHRLVDQ------AEALARLPWGYKPHGSS------------FFINAKLETIERHGWPWKLIGTGRILVPADGWYEWKALDS---GPKPAKQPYYIH--GDAPLLFAGLSAWRRGA-ELDEAHGFAIVTNDALGGMVDVHDRRPVALPPELAREWVDPATPRAKEILRAGLPETAFSWYPVRQEVGSSKYQLPD---------------

2bdvA

0.26

0.21

0.83

2.92

dPPAS

-CGRIAQKSAPEDYVEILWP----NARLVAGPRYNIPPGTRPLTHR------LVDQAEALARLPWGYKPHGSS------------FFINAKLETIERHGWPWKLIGTGRILVPADGWYEWKALDS---GPKPAKQPYYIH--GDAPLLFAGLSAWRRGA-ELDEAHGFAIVTNDALGGMVDVHDRRPVALPPELAREWVDPATPRAKEILRAGLPETAFSWYPVRQEVGSSKYQLPD---------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.94

Estimated TM-score = 0.84±0.08

Estimated RMSD = 3.9±2.7Å

Download Model 2

C-score=-1.67

Download Model 3

C-score=-2.96

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	5ko9A	0.896	1.21	0.321	0.929
2	2f20A	0.773	2.23	0.241	0.857
3	2icuB	0.707	2.60	0.317	0.798
4	1zn6A	0.679	2.67	0.224	0.778
5	2bdvA	0.668	3.04	0.247	0.786
6	2aegA	0.655	3.49	0.177	0.798
7	2wmfA	0.413	6.00	0.068	0.702
8	3h5lB	0.412	5.25	0.064	0.635
9	1ewtA	0.408	5.36	0.053	0.623
10	4ru0A	0.401	5.50	0.060	0.631

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.06	3	1a9x2	III	Rep, Mult	87,91,92,95,151,152
2	0.06	3	1yxiA	MG	Rep, Mult	89,93,119
3	0.04	2	1jdbE	GLN	Rep, Mult	87,90,97,98,104
4	0.04	2	1dxoA	DQN	Rep, Mult	120,151
5	0.04	2	2ibpB	MG	Rep, Mult	193,197
6	0.04	2	2d03L	GOL	Rep, Mult	152,167,168,169
7	0.02	1	3np5A	CA	Rep, Mult	10,13
8	0.02	1	1t64A	CA	Rep, Mult	180,183
9	0.02	1	1jdbH	GLN	Rep, Mult	89,90,91,97,98
10	0.02	1	1aczA	GLC	Rep, Mult	71,120
11	0.02	1	2pvzA	CA	Rep, Mult	91,92,93,172,173
12	0.02	1	2hi8X	BR	Rep, Mult	176,185,186
13	0.02	1	2qenA	MG	Rep, Mult	33,119
14	0.02	1	3jwrA	IBM	Rep, Mult	196,199
15	0.02	1	3kziT	CLA	Rep, Mult	101,105
16	0.02	1	4kitB	MG	Rep, Mult	9,115
17	0.02	1	3fw6A	ZN	Rep, Mult	93,183

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	3msuA	0.368	5.81	0.049	0.607	2.3.3.1	3,218
2	0.060	3djlA	0.397	5.52	0.052	0.619	1.3.99.-	NA
3	0.060	2yyaB	0.379	6.13	0.036	0.663	6.3.4.13	NA
4	0.060	1lrwA	0.382	5.94	0.064	0.647	1.1.99.8	NA
5	0.060	1kb0A	0.382	6.11	0.048	0.671	1.1.99.-	NA
6	0.060	3cdjA	0.360	6.38	0.075	0.655	2.7.7.8	173,237
7	0.060	3d64B	0.329	6.13	0.031	0.579	3.3.1.1	NA
8	0.060	2zf5Y	0.368	6.56	0.056	0.682	2.7.1.30	NA
9	0.060	1yiqA	0.369	6.11	0.034	0.643	1.1.99.-	NA
10	0.060	3edyA	0.392	6.02	0.056	0.686	3.4.14.9	NA
11	0.060	1siqA	0.372	5.54	0.049	0.583	1.3.99.7	NA
12	0.060	1p49A	0.389	6.47	0.059	0.702	3.1.6.2	149
13	0.060	2ys6A	0.372	6.19	0.065	0.667	6.3.4.13	NA
14	0.060	1iphA	0.386	5.99	0.062	0.655	1.11.1.6	111
15	0.060	2wk5A	0.355	6.17	0.096	0.627	2.4.2.4	91
16	0.060	1w93A	0.384	6.77	0.041	0.718	6.3.4.14,6.4.1.2	NA
17	0.060	2iu4B	0.367	5.85	0.053	0.607	2.7.1.2	215
18	0.060	3elqB	0.377	5.66	0.038	0.623	2.8.2.22	38
19	0.060	2ebaA	0.382	5.60	0.035	0.607	1.3.99.7	37

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.49	0.718	2.58	0.31	0.81	2icuB	GO:0006508 GO:0008233 GO:0016787
1	0.06	0.318	5.76	0.01	0.55	5a5b6	GO:0000502 GO:0004175 GO:0004298 GO:0005634 GO:0005737 GO:0005789 GO:0005839 GO:0006508 GO:0008233 GO:0010499 GO:0016787 GO:0019774 GO:0034515 GO:0043161 GO:0051603
2	0.06	0.290	6.19	0.04	0.52	3zkqA	GO:0001540 GO:0004190 GO:0005768 GO:0005783 GO:0005794 GO:0006508 GO:0006509 GO:0008233 GO:0009986 GO:0016020 GO:0016021 GO:0016486 GO:0016787 GO:0030163 GO:0042985 GO:0050435
3	0.06	0.330	6.26	0.06	0.59	4mx6A	GO:0006810 GO:0030288
4	0.06	0.321	6.02	0.05	0.56	3vywD	GO:0016645 GO:0046872 GO:0051536 GO:0051539 GO:0055114
5	0.06	0.297	5.44	0.04	0.47	1zd1A	GO:0004062 GO:0005737 GO:0005829 GO:0006629 GO:0006790 GO:0008146 GO:0008202 GO:0016740 GO:0050427
6	0.06	0.272	6.80	0.04	0.52	1adjA	GO:0000166 GO:0004812 GO:0004821 GO:0005524 GO:0005737 GO:0006412 GO:0006418 GO:0006427 GO:0016874
7	0.06	0.245	6.92	0.03	0.48	3tiiB	GO:0000166 GO:0006464 GO:0046872
8	0.06	0.295	5.67	0.09	0.49	3r1xA	GO:0008671 GO:0016301 GO:0016310 GO:0034194 GO:0046835
9	0.06	0.257	6.32	0.10	0.44	4es6A	GO:0004852 GO:0006779 GO:0006782 GO:0016829 GO:0033014
10	0.06	0.292	6.05	0.07	0.50	1cqxA	GO:0005344 GO:0005737 GO:0006810 GO:0008941 GO:0009636 GO:0015671 GO:0016491 GO:0019825 GO:0020037 GO:0046872 GO:0051409 GO:0055114 GO:0071949
11	0.06	0.305	6.08	0.05	0.52	3b1cA	GO:0003824 GO:0009058 GO:0016829 GO:0030170
12	0.06	0.238	6.18	0.05	0.42	4oxdA	GO:0006508 GO:0008233 GO:0046872
13	0.06	0.239	5.70	0.03	0.40	4ij4A	GO:0004568 GO:0005975 GO:0006032 GO:0016998
14	0.06	0.273	6.04	0.06	0.47	4q66H	GO:0000282 GO:0005794 GO:0006810 GO:0006893 GO:0015031 GO:0016020 GO:0034044 GO:0034221
15	0.06	0.233	6.10	0.06	0.40	4v10A	GO:0002230 GO:0005200 GO:0005737 GO:0005856 GO:0006936 GO:0030017 GO:0031430 GO:0032982 GO:0042802 GO:0042803 GO:0098779 GO:0098792
16	0.06	0.226	6.59	0.06	0.42	3lktM	GO:0003824 GO:0005506 GO:0006725 GO:0008199 GO:0016491 GO:0016702 GO:0018578 GO:0019439 GO:0019619 GO:0042952 GO:0046872 GO:0051213 GO:0055114
17	0.06	0.206	5.95	0.03	0.35	4pn6B	GO:0016020 GO:0016021
18	0.06	0.203	4.68	0.01	0.29	2y23A	GO:0002230 GO:0005737 GO:0005863 GO:0006936 GO:0008307 GO:0030017 GO:0031430 GO:0032982 GO:0042802 GO:0042803 GO:0098779 GO:0098792

Consensus prediction of GO terms

Molecular Function	GO:0008233
GO-Score	0.55
Biological Processes	GO:0006508
GO-Score	0.55
Cellular Component	GO:0030288	GO:0016021	GO:0005634	GO:0009986	GO:0005789	GO:0019774	GO:0005794	GO:0005768	GO:0034515
GO-Score	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.