I-TASSER results

I-TASSER results for job id Rv3212

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (407 residues)
MVKPERRTKTDIAAAATIAVVVAVAASLIWWTSDARATISRPAAVAVPTPAPAREVPTSL
KQLWTAASPATRVPVVVGGTVATGDGRQVDGRDPATGESLWSYARDTDLCGVTWVYHYAV
AVYRYDRGCGQVSTIDGSTGRRGAARSGYADPRVRLFSDGTTVLSAGDTRLELWRSDMVR
MLAYGEIDARVKPSNRGLQSGCTLESAAASSAAVSVLEACTNQADLRLVLLRPGKEDDEP
IQRIVPEPGVRPGSGARVLVVSQNNTAVYLPARSGAQPRVDVIDETGATVSSTLLAKPPS
TSAVASRTGNLVTWWTGDALLVFDAGNLTQRYTIAAGETTAPVGPGVMMAGQLLVPVTGG
IGVYDPVSGANNRYIPVTRPPSTSAVIPAVSGSRVIEQRGDTLVALG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MVKPERRTKTDIAAAATIAVVVAVAASLIWWTSDARATISRPAAVAVPTPAPAREVPTSLKQLWTAASPATRVPVVVGGTVATGDGRQVDGRDPATGESLWSYARDTDLCGVTWVYHYAVAVYRYDRGCGQVSTIDGSTGRRGAARSGYADPRVRLFSDGTTVLSAGDTRLELWRSDMVRMLAYGEIDARVKPSNRGLQSGCTLESAAASSAAVSVLEACTNQADLRLVLLRPGKEDDEPIQRIVPEPGVRPGSGARVLVVSQNNTAVYLPARSGAQPRVDVIDETGATVSSTLLAKPPSTSAVASRTGNLVTWWTGDALLVFDAGNLTQRYTIAAGETTAPVGPGVMMAGQLLVPVTGGIGVYDPVSGANNRYIPVTRPPSTSAVIPAVSGSRVIEQRGDTLVALG
Prediction	CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCCCCCEEHCCEEEEEHCCEEEEEHCCCCCEEEEHCCCCCCCCCCCCCCEEEEEHCCCCCCCEEEEEHCCCCCEEEHCCCCCCCCEEEEEHCCEEEEEHCCCEEEHHHHHHCEEHCCCCCCCCCCCCCCCCCCCEEHCCCCCCCEEEEEEHCCCCCCCEEEEEHCCCCCCCEEEEEEHCCCCCCCCCCEEEEEHCCEEEEEHCCCCCCCEEEEEHCCCCCEEEEEHCCCCCCCCCCCCCCCCEEEEEHCCEEEEEHCCCCCEEEEHCCCCCCCCCCCCEEHCCEEEEEHCCCEEEEHCCCCCEEEEEHCCCCCCCCCEEEEEHCCEEEEEHCCEEEEHC
Conf.Score	99886666467899999999999999999752577776567778888778777789876478887589988786788888999869989998689998799985788766666658879999868998757999988999788764678888757887498899996984454445444334447876666777777788866864555786799999869999858999967899875457886456777788876999968958999778999865899987999689988656788777766567978999978969999689995899985788777778754568889998689579985899977899976899988867778767879999899899859
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MVKPERRTKTDIAAAATIAVVVAVAASLIWWTSDARATISRPAAVAVPTPAPAREVPTSLKQLWTAASPATRVPVVVGGTVATGDGRQVDGRDPATGESLWSYARDTDLCGVTWVYHYAVAVYRYDRGCGQVSTIDGSTGRRGAARSGYADPRVRLFSDGTTVLSAGDTRLELWRSDMVRMLAYGEIDARVKPSNRGLQSGCTLESAAASSAAVSVLEACTNQADLRLVLLRPGKEDDEPIQRIVPEPGVRPGSGARVLVVSQNNTAVYLPARSGAQPRVDVIDETGATVSSTLLAKPPSTSAVASRTGNLVTWWTGDALLVFDAGNLTQRYTIAAGETTAPVGPGVMMAGQLLVPVTGGIGVYDPVSGANNRYIPVTRPPSTSAVIPAVSGSRVIEQRGDTLVALG
Prediction	74444332310000000000000100010000031341444334533554542542153035304354654322110200000023220202225435430315252500000212310000024542002010020533433232445347413112333100000441030022000201312316143526554336403022123342100000104746311011032547755423521443414535402000004510000021664440102011562332233414443445432343320000001320000217503231303336452210101212310000033000001164254423030424424020201011420002345401027
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCCCCCEEHCCEEEEEHCCEEEEEHCCCCCEEEEHCCCCCCCCCCCCCCEEEEEHCCCCCCCEEEEEHCCCCCEEEHCCCCCCCCEEEEEHCCEEEEEHCCCEEEHHHHHHCEEHCCCCCCCCCCCCCCCCCCCEEHCCCCCCCEEEEEEHCCCCCCCEEEEEHCCCCCCCEEEEEEHCCCCCCCCCCEEEEEHCCEEEEEHCCCCCCCEEEEEHCCCCCEEEEEHCCCCCCCCCCCCCCCCEEEEEHCCEEEEEHCCCCCEEEEHCCCCCCCCCCCCEEHCCEEEEEHCCCEEEEHCCCCCEEEEEHCCCCCCCCCEEEEEHCCEEEEEHCCEEEEHC
MVKPERRTKTDIAAAATIAVVVAVAASLIWWTSDARATISRPAAVAVPTPAPAREVPTSLKQLWTAASPATRVPVVVGGTVATGDGRQVDGRDPATGESLWSYARDTDLCGVTWVYHYAVAVYRYDRGCGQVSTIDGSTGRRGAARSGYADPRVRLFSDGTTVLSAGDTRLELWRSDMVRMLAYGEIDARVKPSNRGLQSGCTLESAAASSAAVSVLEACTNQADLRLVLLRPGKEDDEPIQRIVPEPGVRPGSGARVLVVSQNNTAVYLPARSGAQPRVDVIDETGATVSSTLLAKPPSTSAVASRTGNLVTWWTGDALLVFDAGNLTQRYTIAAGETTAPVGPGVMMAGQLLVPVTGGIGVYDPVSGANNRYIPVTRPPSTSAVIPAVSGSRVIEQRGDTLVALG

4hdjA

0.17

0.14

0.83

1.90

MUSTER

----------------------------------------------PPAELTDFKEEVVLSKQWSRSVGDLLEPAVDGSTIYAASEGRVMAIQRETGDVLWKKDLERPVSGGVGVGYGLVLVGTLR---GDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDD-KLIGLDA----ASGDQRWIYESTVPVLTLR-GTGAPLIAGNMALAGL-----ASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDILLLSGD-TLYVVSYQGRAAALDV--NSGRLLWQREAS---SYVGVAEGFGNIYVSQASGSVEGLDSRGASSLWNNDALA-RRQLSAPAVFSSNVVVGDLEGVHLLSQVDGRFVGRERVDSDGVRVR--PLVVGSWMYVFNGGKLVAYT

4hdjA

0.17

0.14

0.83

2.55

dPPAS

----------------------------------------------PPAELTDFKEEVVLSKQWSRDLYNLLEPAVDGSTIYAASEGRVMAIQRETGDVLWKKDLERPVSGGVGVGYGLVLVGTLR---GDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDD-KLIGLDA----ASGDQRWIYESTVPVLTLR-GTGAPLIAGNMALAGL-----ASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIDLLLSGDTLYVVSYQGRAAALDV--NSGRLLWQREAS---SYVGVAEGFGNIYVSQASGSVEGLDSRGASSLWNNDALARRQ-LSAPAVFSSNVVVGDLEGVHLLSQVDGRFVGRERVDSDGVRVR--PLVVGSWMYVFNGGKLVAYT

4hdjA

0.16

0.14

0.83

3.61

wdPPAS

----------------------------------------------PPAELTDFKEEVVLSKQWSRSVGDLLEPAVDGSTIYAASEGRVMAIQRETGDVLWKKDLERPVSGGVGVGYGLVLVGTLR---GDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDDKLI-----GLDAASGDQRWIYESTVPVLTLR-GTGAPLIAGNMALAGL-----ASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIGLLLSGDTLYVVSYQGRAAALDVNSGRLLWQREA-----SSYVGVAEGFGNIYVSQASGSVEGLDSRGASSLWNNDALARRQ-LSAPAVFSSNVVVGDLEGVHLLSQVDGRFVGRERVDSDGVRVR--PLVVGSWMYVFNGGKLVAYT

4hdjA

0.17

0.14

0.83

2.38

wMUSTER

-----------------------------------------------PAELTDFKEEVVLSKQWSRSVGDLLEPAVDGSTIYAASEGRVMAIQRETGDVLWKKDLERPVSGGVGVGYGLVLVGTLR---GDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDD-KLIGLDA----ASGDQRWIYESTVPVLTLRGT-GAPLIAGNMALAGL-----ASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIGLLLSGDTLYVVSYQGRAAALDV--NSGRLLWQREAS---SYVGVAEGFGNIYVSQASGSVEGLDSRGASSLWNNDALAR-RQLSAPAVFSSNVVVGDLEGVHLLSQVDGRFVGRERVDSDGVRVR--PLVVGSWMYVFNGGKLVAYT

4hdjA

0.17

0.14

0.83

3.77

wPPAS

----------------------------------------------PPAELTDFKEEVVLSKQWSRSVGDLLEPAVDGSTIYAASEGRVMAIQRETGDVLWKKDLERPVSGGVGVGYGLVLVGTLR---GDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDD-KLIGLDA----ASGDQRWIYESTVPVLTLRGT-GAPLIAGNMALAGL-----ASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIGLLLSGDTLYVVSYQGRAAALDVNSGRLLWQREA-----SSYVGVAEGFGNIYVSQASGSVEGLDSRGASSLWNNDALARR-QLSAPAVFSSNVVVGDLEGVHLLSQVDGRFVGRERVDSDGVRVR--PLVVGSWMYVFNGGKLVAYT

4hdjA

0.16

0.13

0.83

5.45

dPPAS2

----------------------------------------------PPAELTDFKEEVVLSKQWSRDLYNLLEPAVDGSTIYAASEGRVMAIQRETGDVLWKKDLERPVSGGVGVGYGLVLVGTLR---GDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDD-KLIGLDA----ASGDQRWIYESTVPVLTLR-GTGAPLIAGNMALAGL-----ASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIDLLLSGDTLYVVSYQGRAAALDV--NSGRLLWQREA----SSYVGVAEGFGNIYVSQASSVEGLDSRGASSLWNNDALARRQ-LSAPAVFSSNVVVGDLEGVHLLSQVDGRFVGRERVDSDGVRVR--PLVVGSWMYVFNGGKLVAYT

4hdjA

0.16

0.13

0.83

3.19

PPAS

----------------------------------------------PPAELTDFKEEVVLSKQWSRDLYNLLEPAVDGSTIYAASEGRVMAIQRETGDVLWKKDLERPVSGGVGVGYGLVLVGTLR---GDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDD-KLIGLDA----ASGDQRWIYESTVPVLTLR-GTGAPLIAGNMALAGL-----ASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIGLLLSGDTLYVVSYQGRAAALDV--NSGRLLWQREA----SSYVGVAEGFGNIYVSQASSVEGLDSRGASSLWNNDALARRQ-LSAPAVFSSNVVVGDLEGVHLLSQVDGRFVGRERVDSDGVRVR--PLVVGSWMYVFNGGKLVAYT

4hdjA

0.15

0.13

0.83

4.26

Env-PPAS

----------------------------------------------PPAELTDFKEEVVLSKQWSRSVGDLLEPAVDGSTIYAASEGRVMAIQRETGDVLWKKDLERPVSGGVGVGYGLVLVGTLR---GDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDD-KLIGLDA----ASGDQRWIYESTV-PVLTLRGTGAPLIAGNMALAGL-----ASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIDLLLSGDTLYVVSYQGRAAALDV--NSGRLLWQREAS----SYVGVAEGFGNIYVSQASSVEGLDSRGASSLWNNDALARRQ-LSAPAVFSSNVVVGDLEGVHLLSQVDGRFVGRERVDSDGVRVR--PLVVGSWMYVFNGGKLVAYT

3p1lA

0.17

0.14

0.83

1.86

MUSTER

----------------------------------------------KMSPLPTVENQFTPTTAWSTSVGSGIHPALADNVVYAADAGLVKALNADDGKEIWSVSLPALLSGGVTVSGGHVYIGSEK---AQVYALNTSDGTVAWQTKVAGEALSRPVVSDGLVLIHTSNG-QLQALNE----ADGAVKWTVNLDMPSLSLRGE-SAPTTAFGAAVVG-----GDNGRVSAVLMEQGQMIWQQRISQATGSTEIDRLSDVVVVNG--VVFALAYNGNLTALDL--RSGQIMWKRELG---SVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLH-RLLTSPVLYNGNLVVGDSEGLHWINVEDGRFVAQQKVDSSGFQTEPVAA--GKLLIQAKDGTVYSIT

3p1lA

0.16

0.13

0.84

2.47

dPPAS

----------------------------------------------KMSPLPTVENQFTPTTAWSTSVGSGIHPALADNVVYAADAGLVKALNADDGKEIWSVSLAEKDGWFSKEPAGGVTVSGGGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDGLVLIHTSNGQLQ-----ALNEADGAVKWTVNLDMPSLSLRGESAPTTAFGAAVVG------GDNGRVSAVLMEQGQMIWQQRISQATGSTEIDRLSDVVVVNG--VVFALAYNGNLTALDL--RSGQIMWKRELG---SVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRL-LTSPVLYNGNLVVGDSEGLHWINVEDGRFVAQQKVDSSGFQ--TEPVAADGKLLIQADGTVYSIT

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-0.24

Estimated TM-score = 0.68±0.12

Estimated RMSD = 7.4±4.2Å

Download Model 2

C-score=-0.20

Download Model 3

C-score=-1.39

Download Model 4

C-score=-1.39

Download Model 5

C-score=-2.98

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4hdjA	0.816	1.09	0.165	0.833
2	4pk1A	0.757	2.85	0.136	0.848
3	3p1lA	0.755	2.60	0.153	0.833
4	4maeA	0.726	3.05	0.136	0.823
5	4cvbA	0.726	3.02	0.104	0.823
6	4mh1A	0.723	2.93	0.112	0.816
7	2d0vA	0.723	2.98	0.095	0.821
8	4immA	0.721	2.57	0.139	0.794
9	1kv9A	0.720	3.04	0.140	0.821
10	1yiqA1	0.720	3.04	0.133	0.821

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.10	5	1bl9A	DHE	Rep, Mult	72,73,75,111,112,125,154,155,156,204,262,302,303,345,357,384,389
2	0.06	3	5ealA	5ML	Rep, Mult	72,73,86,109,110,111,112,153,168,202,204,384
3	0.06	3	1nexB	III	Rep, Mult	86,111,125,153,168,204
4	0.04	2	3frpA	CA	Rep, Mult	345,362,363,365
5	0.04	2	2ovqB	III	Rep, Mult	70,111,125,151,152,154,168,204,224,259,316,344,358,384
6	0.02	1	1flgA	CA	Rep, Mult	34,37,40
7	0.02	1	4h5jB	K	Rep, Mult	74,75,113,114,386
8	0.02	1	3mksD	C1C	Rep, Mult	104,105,107,133,134,135,142,144,145
9	0.02	1	4nbmD	MG	Rep, Mult	73,384
10	0.02	1	3e4qA	CA	Rep, Mult	96,97,101
11	0.02	1	1ztzA	III	Rep, Mult	365,369
12	0.02	1	3pe7A	CA	Rep, Mult	324,326
13	0.02	1	1yfqA	CA	Rep, Mult	401,402
14	0.02	1	3ebzB	IMD	Rep, Mult	386,397
15	0.02	1	2z2pA	DOL	Rep, Mult	265,284,285
16	0.02	1	3c9iC	XE	Rep, Mult	132,135

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.067	1ri6A	0.561	4.29	0.084	0.705	3.1.1.31	NA
2	0.067	1g72A	0.718	3.05	0.108	0.821	1.1.99.8	NA
3	0.067	2h47H	0.555	4.71	0.078	0.727	1.4.99.4	75
4	0.067	3c75J	0.551	4.93	0.074	0.730	1.4.99.3	75,78,94
5	0.066	2gc7A	0.547	4.55	0.087	0.695	1.4.99.3	75,78,93
6	0.060	1gjqA	0.679	4.03	0.068	0.821	1.7.99.1,1.7.2.1	NA
7	0.060	2ebsB	0.574	4.65	0.083	0.749	3.2.1.150	93
8	0.060	1yiqA	0.633	3.99	0.091	0.771	1.1.99.-	151
9	0.060	2d0vA	0.723	2.98	0.095	0.821	1.1.99.8	151
10	0.060	1w6sC	0.718	3.08	0.117	0.821	1.1.99.8	151
11	0.060	1fwxA	0.540	4.38	0.082	0.688	1.7.99.6	NA
12	0.060	1lrwC	0.726	3.04	0.122	0.823	1.1.99.8	NA
13	0.060	1v0eA	0.500	4.87	0.075	0.676	3.2.1.129	NA
14	0.060	1madH	0.547	4.77	0.062	0.715	1.4.99.3	121,135
15	0.060	1e5tA	0.551	4.87	0.100	0.730	3.4.21.26	NA
16	0.060	1kv9A	0.720	3.04	0.140	0.821	1.1.99.-	151
17	0.060	2bixA	0.514	5.26	0.066	0.705	1.14.99.-	80
18	0.060	1orvA	0.563	5.07	0.072	0.769	3.4.14.5	NA
19	0.060	1sllA	0.505	5.27	0.050	0.693	4.2.2.15	NA
20	0.060	2d5lA	0.573	4.64	0.058	0.747	3.4.14.-	NA
21	0.060	1z4zA	0.507	5.17	0.041	0.700	3.2.1.18	NA
22	0.060	2jkbA	0.509	5.17	0.054	0.690	3.2.1.18,4.2.2.15	NA
23	0.060	2bklB	0.552	4.62	0.083	0.725	3.4.21.26	NA
24	0.060	2gbcA	0.599	4.87	0.060	0.801	3.4.14.5,3.4.15.5	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.31	0.816	1.09	0.17	0.83	4hdjA	GO:0009279 GO:0016020 GO:0043165 GO:0051205
1	0.26	0.757	2.85	0.14	0.85	4pk1A	GO:0007155 GO:0009279 GO:0016020 GO:0016021 GO:0019867 GO:0042802 GO:0043165 GO:0051205 GO:0071709 GO:1990063
2	0.12	0.726	3.05	0.14	0.82	4maeA	GO:0005509 GO:0016020 GO:0016491 GO:0016614 GO:0052930 GO:0052931 GO:0052932 GO:0052933 GO:0052934 GO:0052935 GO:0052936 GO:0055114
3	0.12	0.726	3.02	0.10	0.82	4cvbA	GO:0046872
4	0.12	0.633	3.99	0.09	0.77	1yiqA	GO:0005509 GO:0009055 GO:0016020 GO:0016491 GO:0016614 GO:0020037 GO:0042597 GO:0046872 GO:0055114 GO:0070968
5	0.12	0.724	2.98	0.11	0.82	1lrwA	GO:0005509 GO:0015945 GO:0016020 GO:0016491 GO:0016614 GO:0030288 GO:0042597 GO:0046872 GO:0052930 GO:0052931 GO:0052932 GO:0052933 GO:0055114
6	0.12	0.718	3.08	0.12	0.82	1w6sC	GO:0005509 GO:0005886 GO:0015945 GO:0016020 GO:0016491 GO:0016614 GO:0030288 GO:0046872 GO:0052930 GO:0052931 GO:0052932 GO:0052933 GO:0055114
7	0.12	0.719	3.08	0.11	0.82	1flgA	GO:0005509 GO:0006069 GO:0016020 GO:0016491 GO:0016614 GO:0018468 GO:0030288 GO:0042597 GO:0046872 GO:0052934 GO:0052935 GO:0052936 GO:0055114
8	0.12	0.725	3.03	0.12	0.82	4tqoA	GO:0005509 GO:0015946 GO:0016020 GO:0016614 GO:0018468 GO:0030288 GO:0046872 GO:0055114
9	0.12	0.721	2.57	0.14	0.79	4immA	GO:0009279 GO:0016020 GO:0043165 GO:0046872 GO:0051205
10	0.11	0.723	2.98	0.10	0.82	2d0vA	GO:0005509 GO:0016020 GO:0016614 GO:0030288 GO:0046872 GO:0055114
11	0.11	0.718	3.07	0.11	0.82	4aahA	GO:0005509 GO:0005886 GO:0015945 GO:0016020 GO:0016491 GO:0016614 GO:0030288 GO:0046872 GO:0052930 GO:0052931 GO:0052932 GO:0052933 GO:0055114
12	0.11	0.630	4.02	0.11	0.77	1kb0A	GO:0005509 GO:0009055 GO:0016020 GO:0016491 GO:0016614 GO:0020037 GO:0030288 GO:0042597 GO:0046872 GO:0055114
13	0.07	0.720	3.04	0.14	0.82	1kv9A	GO:0005509 GO:0009055 GO:0016020 GO:0016491 GO:0016614 GO:0020037 GO:0030288 GO:0042597 GO:0046872 GO:0055114 GO:0070968
14	0.06	0.324	7.13	0.07	0.56	2du2A	GO:0000166 GO:0003824 GO:0010181 GO:0016491 GO:0046872 GO:0055114
15	0.06	0.305	6.52	0.06	0.48	4k3cA	GO:0009279 GO:0016020 GO:0016021 GO:0019867 GO:0043165 GO:0051205 GO:0071709
16	0.06	0.260	7.26	0.04	0.45	4xlaA	GO:0009405
17	0.06	0.254	7.17	0.05	0.44	3g0tA	GO:0003824 GO:0008483 GO:0009058 GO:0016740 GO:0030170
18	0.06	0.258	7.38	0.05	0.45	2y4oA	GO:0000166 GO:0003824 GO:0008152 GO:0010124 GO:0016874 GO:0046872 GO:0047475

Consensus prediction of GO terms

Molecular Function	GO:0005515	GO:0016491	GO:0046872
GO-Score	0.52	0.46	0.46
Biological Processes	GO:0022610	GO:0051205	GO:0043165	GO:0044710
GO-Score	0.52	0.49	0.49	0.46
Cellular Component	GO:0031246	GO:0098796
GO-Score	0.52	0.52

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.