I-TASSER results

I-TASSER results for job id Rv3190c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (421 residues)
MEYVQLFSKGRLNDLAGSLAGFLGKASQATAQRLQSWDADDLLNTPVDDVVEQLVELGSV
ECPDLRVDDAFMLPATEVDQQYRDWGEQRTRRVTRLVLVVPFEGHKDIFNLRPDQFTTMP
PQVLRLQGHEIHLAIDNLSNDAAAINAAFHKQIANIEKYLGWSRRQIDLHNQGLRNELPG
MVARRREQLLATRNLQAEIGFPVRRRKDADTYAAPISRKSVRPRPHRPAGARAAFKPEPA
MQDEDYQSALRVLRNQRNALERTPSVAAKLDGEEIRDMLLVGLNAQFEGDAGGELFNGAG
KTDILIRVDDRNIFIGECKVWSGPRTMDDVLKQLFGYLVWRDTKAAILLFIRNKDVTAVI
DNAIAKIKEHPNHKRCPAHRAGADQYEFTMHADGDPEREIHLTLIPFALRPTAEVPTTTI
P

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MEYVQLFSKGRLNDLAGSLAGFLGKASQATAQRLQSWDADDLLNTPVDDVVEQLVELGSVECPDLRVDDAFMLPATEVDQQYRDWGEQRTRRVTRLVLVVPFEGHKDIFNLRPDQFTTMPPQVLRLQGHEIHLAIDNLSNDAAAINAAFHKQIANIEKYLGWSRRQIDLHNQGLRNELPGMVARRREQLLATRNLQAEIGFPVRRRKDADTYAAPISRKSVRPRPHRPAGARAAFKPEPAMQDEDYQSALRVLRNQRNALERTPSVAAKLDGEEIRDMLLVGLNAQFEGDAGGELFNGAGKTDILIRVDDRNIFIGECKVWSGPRTMDDVLKQLFGYLVWRDTKAAILLFIRNKDVTAVIDNAIAKIKEHPNHKRCPAHRAGADQYEFTMHADGDPEREIHLTLIPFALRPTAEVPTTTIP
Prediction	CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCEHCCEEEHCCCEEHCCCHCCCCCCCCCCCEEEEEEEEEEEEEHCCCHHHHHHCCCCCCCCCCCEEEHCCCEEEEEEEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHCCCCCCCCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEHCCCCCCEEEEEHCCCEEEEEEEEHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEHCCCCCCCEEEEEEEEEHCCCCCCCCCCCCC
Conf.Score	9845544467655455569999999999999999859977754799999999999984444646655554554554456656677875678668999999867875676536888877776568985988999999678988999999999999999999999999999858999999999999999999999999997885455789987667765455556788887777888999878888999999999999999986978887589789999999874666677655456657887569999889868999986548855799999999975778787689999984766899999999999977655655566788867999996789888689999999857887667887789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MEYVQLFSKGRLNDLAGSLAGFLGKASQATAQRLQSWDADDLLNTPVDDVVEQLVELGSVECPDLRVDDAFMLPATEVDQQYRDWGEQRTRRVTRLVLVVPFEGHKDIFNLRPDQFTTMPPQVLRLQGHEIHLAIDNLSNDAAAINAAFHKQIANIEKYLGWSRRQIDLHNQGLRNELPGMVARRREQLLATRNLQAEIGFPVRRRKDADTYAAPISRKSVRPRPHRPAGARAAFKPEPAMQDEDYQSALRVLRNQRNALERTPSVAAKLDGEEIRDMLLVGLNAQFEGDAGGELFNGAGKTDILIRVDDRNIFIGECKVWSGPRTMDDVLKQLFGYLVWRDTKAAILLFIRNKDVTAVIDNAIAKIKEHPNHKRCPAHRAGADQYEFTMHADGDPEREIHLTLIPFALRPTAEVPTTTIP
Prediction	7522400341405434440242045115502630572556301414244004202750304104043561423445424551444545232402101010103113300412133222332322414642010102245442540354045105404610530364055216503520462055115403644511550512045366154241235445241554435456451543230447304400400340043024225305736253001000000132252512110024512010102166420000001214145312500400021032442200000002345144105403520572441473446455533020203347444230100000010133151443527
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCEHCCEEEHCCCEEHCCCHCCCCCCCCCCCEEEEEEEEEEEEEHCCCHHHHHHCCCCCCCCCCCEEEHCCCEEEEEEEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHCCCCCCCCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEEHCCCCCCEEEEEHCCCEEEEEEEEHCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCEEEEEEHCCCCCCCEEEEEEEEEHCCCCCCCCCCCCC
MEYVQLFSKGRLNDLAGSLAGFLGKASQATAQRLQSWDADDLLNTPVDDVVEQLVELGSVECPDLRVDDAFMLPATEVDQQYRDWGEQRTRRVTRLVLVVPFEGHKDIFNLRPDQFTTMPPQVLRLQGHEIHLAIDNLSNDAAAINAAFHKQIANIEKYLGWSRRQIDLHNQGLRNELPGMVARRREQLLATRNLQAEIGFPVRRRKDADTYAAPISRKSVRPRPHRPAGARAAFKPEPAMQDEDYQSALRVLRNQRNALERTPSVAAKLDGEEIRDMLLVGLNAQFEGDAGGELFNGAGKTDILIRVDDRNIFIGECKVWSGPRTMDDVLKQLFGYLVWRDTKAAILLFIRNKDVTAVIDNAIAKIKEHPNHKRCPAHRAGADQYEFTMHADGDPEREIHLTLIPFALRPTAEVPTTTIP

5dlqB

0.10

0.99

0.74

MUSTER

IEGLNTINGIEIEDSEVGISSIISNLITVFRNVLTAIPSELFSSFCLTHLTCSFGRSAALEEVLDKDDMVYMEAYDKLLESWLTLVRKGFFTQHAVQVF-----NSYIQCHLAAPDGTRNLTANGVASREEEEISELQEDDRDQFSDQLASVGMLGRIAAEHCMPLLTSLLEERVTRLHGQLQRHQQDNKMLDDLYEDIHWLLADDTQGETPLIPKHSSEVDINTTLQILGSPGEKASSIPGYSRTDSVIRLLSAVLRVSEVESRAIRADLSPQMGKDIVWFLKRWAKTYLLVDEKLYDQISLPLSTAFGAGYLLQKVSVWSSEQDLANDTVQLLVTLVERRERANLVIQCENWNLAKQFASRSPPLNFLSSVLGGFAHMDTETKQQYRFLMCQQEEVKQEITATLEALCGIAETQIDNVA

5da9A

0.07

0.89

0.76

dPPAS

HPETIAFNTPLVGYNGSGKTTVIECLKYATTGELPPNSTRNGAFIHDPDLVGEKEVRAQVKLSFRSTIGESYV-VTRNIQLLVQRNNKRTQKTLEGSLLLRNNG-----------------------------ERTVISTRVAELDKLVSEKLGVPPAILDAVPSEPAALKKRFDEIFEAQKYTKVIENIRLLKKKKGDELKILKEREVQDKANKERAEKVDELDLKDAKAKYKETHIKVETTKAAIEDLGRGAAVDHAIQYHSKEQINRTIAELWQSTYQGTDIDTIQIRSDVESTTSSTRRNYNYRVSVKGDTEDRGRCSAGQKVLASIIIRLALAESFCANCGLIALDEPTTLAESLHGIIKARQAQGNEFLKYQCSDFCDDFYRVKRDEKQNSVIVRESIT----------------

5lq4A

0.11

0.10

0.89

0.66

wdPPAS

----DLFT--SFSPDAEQLRLILEHPQPGLRTALEQLKQG---NLTLAQLTELVSEQDGVEAGITFASELEKLVDL----------GWICHSVLPLITAIPIAKDYELSSWQTTAIALSRFAFLHQDLQQLVLESPRSKSKLVILDWRVGAVIAKLAQSDRGFI-------ATSADSLLADLSLELEELKRLFALLIATQMMDLEPEDETTRLLPVFEHRDRYPYVKPVISTQAIPVKPDLTALATTDLTEAIET------RSIREYSDITLAQLGEFLYRCARLPEDPMQVGESTTRGG-LEIYPLVHQAGLYHYQ-TL-DWTPEVESLVYDAWRATGQQSIPQIVLIITARKHVGVLYQTFYLVATAM-QLAPSAIGAGNTTKFCQIAGL--NPDEEASVGEFSLGAAK---------P

3w3tA

0.11

0.96

0.67

wMUSTER

MECATLIGFAKFHEHSQELISILVALQNS-DALRSYLLGDDFVPL-LPIVIPPLLITAKATQDVGLIEEEEAANFQQYPDWDVVQVQGKHIAIHTSVLDDKVSAMELLQSYATLLRGQFAVYVKEV--EEIALPSLDFYLH-DGVRAAGATLIPILLSCLLAATEELVLLWHKASSKLIGGLMSMPEITQVYHNSLVNGIKVMGDNCLSEDQLAAFTKGVSANLTDTYERMQDRDEYNEFTDEDLLDEINKSIAAVLKTTNGHPMINTFLNEPILVIFALVVIGDLIQYE-QTASMKNA----FILISPDARIIIGVCAQYAPSDVCIPTLDTLVQIVKLEENRSST------ENASAAIAKILYAYDTYANWFKTLPTITDKEAASFNYQFENSNISAV-VDSVIQALNERSFLPSNRYP

5lq4A

0.12

0.10

0.84

0.63

wPPAS

----DLFT--SFSP-AEQLRLILEHPQPGLRTALEQLKQG---NLTLAQLTELVSEQDGVEAGITFASELEKLVD----------LGWICHSVLPLITAIPIAKDYELSSWQTTAIALSRFAFLHQDLQQLVLESKLVILDVGAVIAKLAQRGFIFATSADSLLADLSLELEELKRLFALLIATQ----------------MMDLEPEDETITQPVFEHRDRYPYVKPVISTQAIPVKPDLTALATTDLTEAIET------RSIREYSDITLAQLGEFLYRCATLPEDPMQVGESTTRGG-LEIYPLVHQGDL---AAGLYDWTPEVESLVYDAWRATGQQSIPQIVLIITARKHVGVLYQTFYLVATAM-----------NTTKFCQIAGL--NPDEEASVGEFSLGAAK---------P

4fmyA

0.08

0.03

0.35

0.97

dPPAS2

-----------------------------------------------------------------------------------------------------------------------------VKNDEQDVELDARIAANTKAINILEVRLTTAEGKIVVLRSDVDYLLDEVIDIQAHLVTVDQRLDGVESDVSDIKSYVSKTVTESQSLASPLDVKTSGTPLLGSFNANQSYTVGTTYTQSEVAALATGLEQARQRILALETALRLHGD-----------------------------------------------------------------------------------------------------------------------------------------------------

5da9A

0.09

0.08

0.89

0.79

PPAS

HHPETIFNTP-IVGYNGSKTTVIECLKYATTGELPPNSTRNGAFIHDPDLVGEKEVRAQVKLSFRSTIGESYV-VTRNIQLLVQRNNKRTQKTLEGSLLLRNNG-----------------------------ERTVISTRVAELDKLVSEKLPAILDAVIFCHSEPAALKKRFDEIFEAQKYTKVIENIRLLKKKKGDELKILKEREVQDKANKERAEKVDELDLKDAKAKYKETHIKVETTKAAIEDLGRGAAVDHAIQYHSKEQINRTIAELWQSTYQGTDIDTIQIRSDVESTTSSTRRNYNYRVSVKGDTEDRGRCSAGQKVLASIIIRLALAESFCANCGLIALDNIRSLAESLHGIIKARQAQGNFLKYQCSDFCD--DFYRVKRDEKQNSVIVRESIT---------------

5gkeA

0.16

0.09

0.54

0.71

Env-PPAS

----------------------------------SKDKVTVITSPSTEELVSLVNSA-------LLEEAMLTIFARCK-VHYDGRAKSELGSGDRVIIVKP---DGSFLIHQSKKREQPPGSRVRLELRENPVLVSIRRKPRETLEVELEEVYM----------------------------------------------VSVFRAEDYEELALTGS------------------------------------------------------EAEMAELIFENPEVIEPGFKFREKAIGTGIVAVLGRDSDGNIVVLELKRRRAE---LHAVRQLKSYVEIYGDKVRGILVAPS-----LTSGAKRLLEKEG--------------LEFRKL---EPPKR----------------------

5b43A

0.10

0.94

0.72

MUSTER

KENCHIFTR--LITAVPSLREHFENVKKAIIEEVFSFPYNQLLTQTLNEVLNLAIQKNDIASLPHRFIPLFLSDRNTLSFILEEFKSDEEVIQSFCKYKTLLENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRIS-KSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLKKQEEKEILKSQLDDWFAVDESNEVDPEF-SARLTGIKLEMEPSLSFYNKARNYATKKSVEKFK------LNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKALS-----FEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTP---------ILLSNNFIEPLEITKEIYDLNNPEKEPKKFQT

4cgkA

0.07

0.06

0.80

0.73

dPPAS

---------SNLTAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGE--ITELSKNIVSRNQSLEKQARSAQTNGAVTSY---------------INTIVNSKSITEAI---------------------SRVAAMSEIVSANNKMLEQQKADKKAISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLLEQKAAAE----------AEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKVRPTYSTNAS-------------------SYPIGECTWGVKTLAPWAGDYWG---------NGAQWATSAAAAGFRTGSTPQVGAIACWNDGGYGHVAVVTAVESTTRIQVSESNYAGHRGWFNPTTTSE

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.68

Estimated TM-score = 0.31±0.10

Estimated RMSD = 16.2±3.1Å

Download Model 2

C-score=-4.58

Download Model 3

C-score=-3.41

Download Model 4

C-score=-4.02

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	5da9A	0.800	2.52	0.075	0.862
2	3eukH	0.568	5.03	0.067	0.758
3	3qf7A	0.562	4.40	0.071	0.703
4	4ux3A	0.553	4.65	0.063	0.717
5	3zgxA	0.540	4.56	0.067	0.703
6	3qktA	0.532	4.49	0.076	0.677
7	1w1wB	0.516	4.10	0.088	0.637
8	4i99A	0.501	4.04	0.114	0.611
9	4abyA	0.489	5.33	0.064	0.670
10	2o5vA	0.453	5.07	0.098	0.608

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.11	6	3thoA	MG	Rep, Mult	20,352
2	0.06	3	5dacA	AGS	Rep, Mult	19,20,21,43,45,47,50,391
3	0.06	3	3qktB	ANP	Rep, Mult	283,284,320,322,323,324,325
4	0.04	2	1eefD	UUU	Rep, Mult	300,301
5	0.04	2	3odhF	CA	Rep, Mult	303,317
6	0.02	1	3c25B	CA	Rep, Mult	294,303
7	0.02	1	2hgu5	III	Rep, Mult	205,208,209,212,215,216
8	0.02	1	3c2mA	MN	Rep, Mult	302,303
9	0.02	1	3odhA	CA	Rep, Mult	288,303
10	0.02	1	5dacB	AES	Rep, Mult	274,275,335,338,339,348,367
11	0.02	1	1dbrB	MG	Rep, Mult	317,329
12	0.02	1	1vs82	III	Rep, Mult	327,331,334,335,337,359,360
13	0.02	1	2hbkA	MN	Rep, Mult	294,342
14	0.02	1	3dpgA	CA	Rep, Mult	303,350
15	0.02	1	2hgu3	III	Rep, Mult	322,323,324,354

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1gowA	0.342	7.32	0.069	0.587	3.2.1.23	NA
2	0.060	1gonA	0.334	7.18	0.057	0.561	3.2.1.21	NA
3	0.060	1tr1D	0.337	7.19	0.038	0.570	3.2.1.21	174
4	0.060	3g61A	0.334	5.31	0.108	0.463	3.6.3.44	NA
5	0.060	1yiqA	0.343	6.54	0.024	0.539	1.1.99.-	NA
6	0.060	2b3xA	0.271	7.42	0.024	0.473	4.2.1.3	NA
7	0.060	1bgaA	0.338	7.21	0.038	0.572	3.2.1.21	NA
8	0.060	1vffA	0.343	7.22	0.038	0.587	3.2.1.21	NA
9	0.060	1od0B	0.331	7.34	0.031	0.570	3.2.1.21	NA
10	0.060	2vumA	0.330	7.10	0.044	0.553	2.7.7.6	NA
11	0.060	1qoxA	0.333	7.40	0.045	0.577	3.2.1.21	NA
12	0.060	3h0gA	0.337	7.14	0.055	0.568	2.7.7.6	275,338
13	0.060	2w00B	0.334	7.06	0.042	0.556	3.1.21.3	333
14	0.060	3gnrA	0.335	7.22	0.047	0.563	3.2.1.21	NA
15	0.060	2dgaA	0.330	7.27	0.040	0.563	3.2.1.21	NA
16	0.060	1gnxA	0.331	7.45	0.047	0.572	3.2.1.21	NA
17	0.060	3kx2B	0.331	6.30	0.067	0.508	3.6.4.13	175
18	0.060	2ipyA	0.275	6.88	0.043	0.449	4.2.1.3	NA
19	0.060	1dwaM	0.336	7.32	0.041	0.575	3.2.1.147	172

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.16	0.804	2.55	0.07	0.87	5da9A	GO:0000723 GO:0005089 GO:0005524 GO:0005634 GO:0006281 GO:0016887 GO:0030870 GO:0035023 GO:0043547
1	0.11	0.727	2.51	0.07	0.78	5da9B	GO:0000723 GO:0005089 GO:0005524 GO:0005634 GO:0006281 GO:0016887 GO:0030870 GO:0035023 GO:0043547
2	0.07	0.590	3.85	0.10	0.71	3qkuA	GO:0000166 GO:0005524 GO:0006281 GO:0006302 GO:0006974 GO:0008270 GO:0016787 GO:0016887 GO:0042802 GO:0046872
3	0.07	0.562	4.40	0.07	0.70	3qf7A	GO:0000166 GO:0005524 GO:0006281 GO:0006974 GO:0016787 GO:0046872
4	0.07	0.553	4.65	0.06	0.72	4ux3A	GO:0000166 GO:0003682 GO:0005524 GO:0005634 GO:0005694 GO:0006281 GO:0007049 GO:0007064 GO:0007067 GO:0007130 GO:0007131 GO:0008280 GO:0016887 GO:0030437 GO:0034990 GO:0042802 GO:0051177 GO:0051276 GO:0051301 GO:1990414
5	0.07	0.532	4.49	0.08	0.68	3qktA	GO:0000166 GO:0005524 GO:0006281 GO:0006302 GO:0006974 GO:0008270 GO:0016787 GO:0016887 GO:0042802 GO:0046872
6	0.07	0.518	3.82	0.09	0.63	3qktB	GO:0000166 GO:0005524 GO:0006281 GO:0006302 GO:0006974 GO:0008270 GO:0016787 GO:0016887 GO:0042802 GO:0046872
7	0.07	0.516	4.00	0.11	0.63	4i99B	GO:0000166 GO:0003677 GO:0005524 GO:0005694 GO:0005737 GO:0006260 GO:0007059 GO:0007062 GO:0030261 GO:0051276
8	0.07	0.453	5.07	0.10	0.61	2o5vA	GO:0000166 GO:0003677 GO:0003697 GO:0005524 GO:0005737 GO:0006260 GO:0006281 GO:0006302 GO:0006974 GO:0009432
9	0.07	0.455	5.37	0.05	0.62	3auyA	GO:0000166 GO:0005524 GO:0006281 GO:0006302 GO:0006974 GO:0008270 GO:0016787 GO:0016887 GO:0046872
10	0.06	0.395	4.33	0.07	0.50	1e69A	GO:0000166 GO:0003677 GO:0005524 GO:0005694 GO:0005737 GO:0006260 GO:0007059 GO:0007062 GO:0030261 GO:0042802 GO:0051276
11	0.06	0.340	7.49	0.04	0.59	2iw3B	GO:0000166 GO:0001933 GO:0003723 GO:0003746 GO:0005524 GO:0005737 GO:0005840 GO:0006412 GO:0006414 GO:0006415 GO:0006469 GO:0010494 GO:0016887 GO:0019843 GO:0022626 GO:0042788
12	0.06	0.343	4.89	0.07	0.46	1b0uA	GO:0000166 GO:0003333 GO:0005524 GO:0005886 GO:0006810 GO:0006865 GO:0015424 GO:0016020 GO:0016887
13	0.06	0.328	4.79	0.05	0.44	3c41J	GO:0000166 GO:0003333 GO:0005524 GO:0015424 GO:0015426 GO:0016887
14	0.06	0.323	4.68	0.05	0.43	4huqB	GO:0000166 GO:0005524 GO:0005886 GO:0006810 GO:0016020 GO:0016787 GO:0016887 GO:0043190
15	0.06	0.327	6.54	0.03	0.51	3zqjD	GO:0000166 GO:0003677 GO:0003824 GO:0004518 GO:0005524 GO:0005618 GO:0005737 GO:0005829 GO:0005886 GO:0006281 GO:0006289 GO:0006974 GO:0008270 GO:0009380 GO:0009381 GO:0009432 GO:0016887 GO:0046872 GO:0060543 GO:0090305
16	0.06	0.344	6.18	0.05	0.52	3zqjA	GO:0000166 GO:0003677 GO:0003824 GO:0004518 GO:0005524 GO:0005618 GO:0005737 GO:0005829 GO:0005886 GO:0006281 GO:0006289 GO:0006974 GO:0008270 GO:0009380 GO:0009381 GO:0009432 GO:0016887 GO:0046872 GO:0060543 GO:0090305
17	0.06	0.338	4.99	0.07	0.45	4ry2A	GO:0005524 GO:0006508 GO:0006810 GO:0008233 GO:0008234 GO:0016020 GO:0016021 GO:0016887 GO:0022885 GO:0042626 GO:0043213 GO:0055085
18	0.06	0.330	4.57	0.04	0.43	2pcjA	GO:0000166 GO:0005524 GO:0005886 GO:0006810 GO:0016020 GO:0016787 GO:0016887 GO:0042953 GO:0042954 GO:0043190

Consensus prediction of GO terms

Molecular Function	GO:0005088	GO:0005524	GO:0016887
GO-Score	0.51	0.39	0.35
Biological Processes	GO:0060249	GO:0032200	GO:0051345	GO:0046578	GO:0043087	GO:0007266	GO:0006281
GO-Score	0.51	0.51	0.51	0.51	0.51	0.51	0.39
Cellular Component	GO:0043234	GO:0044428
GO-Score	0.51	0.51

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.