I-TASSER results

I-TASSER results for job id Rv3099c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (283 residues)
MTTPGRPLTTLDKSDVLAGLFAVWHSLDALLDGLLETDWQATSPLPGWDVKAVVSHIIGT
ESFLLGIAAPEPDTDVSALAHVRNPIGVMNECWVRHLGTESGVGLLERFRAVTSQRRKVL
ASLSDDEWNAPTTTPSGPDSYGRFMRIRIFDCWMHEQDIRAAVQRPSSDDELGGPASPLV
LDEIAATMGFVVGKLAKAPDGSRVLLELTGPLSRSIRVSVDGRARVVDDFGGPAPTATIR
LDGLQFTRLAGGRPMSPARSQDVELGGDKELAGHILERLNFVI

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTTPGRPLTTLDKSDVLAGLFAVWHSLDALLDGLLETDWQATSPLPGWDVKAVVSHIIGTESFLLGIAAPEPDTDVSALAHVRNPIGVMNECWVRHLGTESGVGLLERFRAVTSQRRKVLASLSDDEWNAPTTTPSGPDSYGRFMRIRIFDCWMHEQDIRAAVQRPSSDDELGGPASPLVLDEIAATMGFVVGKLAKAPDGSRVLLELTGPLSRSIRVSVDGRARVVDDFGGPAPTATIRLDGLQFTRLAGGRPMSPARSQDVELGGDKELAGHILERLNFVI
Prediction	CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHCCCCCEEEEEHCCCCEEHCCCCCCCCCEEEEHCHHHHHHHHHCCCCHHHCCCCEEEHCCHHHHHHHHHHCCCCC
Conf.Score	9999888888898999999999999999999769988857888899998999999899999999865567777776654665577556688999987589989999999999999999999788777877556788887789999999999999999999987999998754555789999999998677776557789997589999868997899997788656657889998689997999999999899985555567799757999999999878789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTTPGRPLTTLDKSDVLAGLFAVWHSLDALLDGLLETDWQATSPLPGWDVKAVVSHIIGTESFLLGIAAPEPDTDVSALAHVRNPIGVMNECWVRHLGTESGVGLLERFRAVTSQRRKVLASLSDDEWNAPTTTPSGPDSYGRFMRIRIFDCWMHEQDIRAAVQRPSSDDELGGPASPLVLDEIAATMGFVVGKLAKAPDGSRVLLELTGPLSRSIRVSVDGRARVVDDFGGPAPTATIRLDGLQFTRLAGGRPMSPARSQDVELGGDKELAGHILERLNFVI
Prediction	6546743345344640140034014202400560457205211306632001000000210210111234445432431441343132112200442373425401420351143024104404534242313122111221310201000000321000101424343642342102100210030123002441523644302020201332302021443323134355641302030402100100141243643354042420450032005204024
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHCCCCCEEEEEHCCCCEEHCCCCCCCCCEEEEHCHHHHHHHHHCCCCHHHCCCCEEEHCCHHHHHHHHHHCCCCC
MTTPGRPLTTLDKSDVLAGLFAVWHSLDALLDGLLETDWQATSPLPGWDVKAVVSHIIGTESFLLGIAAPEPDTDVSALAHVRNPIGVMNECWVRHLGTESGVGLLERFRAVTSQRRKVLASLSDDEWNAPTTTPSGPDSYGRFMRIRIFDCWMHEQDIRAAVQRPSSDDELGGPASPLVLDEIAATMGFVVGKLAKAPDGSRVLLELTGPLSRSIRVSVDGRARVVDDFGGPAPTATIRLDGLQFTRLAGGRPMSPARSQDVELGGDKELAGHILERLNFVI

2nsgA

0.13

0.11

0.84

1.59

MUSTER

MTT----FHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETPM----YVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTAQGRVPASETLWMRSREVWIHAVDLG----AVATFGDIPEVILRTLAAEITQKWTSQG-------------------AGEGLVLLDEPSSTRY-PAAPGQDEVVVSGSLAGIVRYAAGR-----GSDGVTSSTGE-----VPEPPRW--

2nsgA

0.13

0.11

0.82

1.57

dPPAS

----MTTFHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETP----MYVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTAQGRVPASETLWMRSREVWIHAVDLG--------AVATFGDIPEVILRTLAAEITQKWTSQGAGEG----------------LVLLDEPSSTRYPAAPGQDEVVVSGSLAGIVRYAAGRGSDGVTSSTGEV------------------

2nsgA

0.11

0.10

0.84

3.32

wdPPAS

MTT----FHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETP----MYVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVTAQGRTVPASETLWMRSREVWIHAVDLGAVATFGDIPEVILRTLAAEITQKWTSQ------------------------AGEGLVLLDEPSSTRY-PAAPGQDEVVVSGSLAGIVRYAAGRGS----DGVTSSTGEVPEPPRW--------

2nsgA

0.12

0.10

0.82

1.98

wMUSTER

TT-----FHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETPM----YVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTAQGRVPASETLWMRSREVWIHAVDLGAVAT----FGDIPEVILRTLAAEITQKWTSQGAGEG-------------------LVLLDEPSSTRY-PAAPGQDEVVVSGSLAGIVRYAAGRGSDGVTSSTGEV------------------

2nsgA

0.14

0.12

0.84

3.65

wPPAS

MTT----FHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETP----MYVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVTAQGRTVPASETLWMRSREVWIHAVDLGAVATFGDIPEVILRTLAAEITQKWTSQ----------AGEG---LVLLDEPSSTRYPA------------APGQDEVVVSGSLAGIVRYAAGRGSDGVTSST----GEVPEPPRW--------

2nsgA

0.15

0.12

0.82

4.14

dPPAS2

MTT----FHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETP----MYVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTAQGRVPASETLWMRSREVWIHAVDLGAVAT--------FGDIPEVILRTLAAEITQKWTSQGAGEG----------------LVLLDEPSSTRYPAAPGQDEVVVSGSLAGIVRYAAGRGSDGVTSSTGEV------------------

2nsgA

0.15

0.13

0.84

2.57

PPAS

MTT----FHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETP----MYVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTAQGRVPASETLWMRSREVWIHAVDLGAVATFGDIP--------EVILRTLAAEITQKWTSQGAGEG----LVLLDEPSSTRYPA------------APGQDEVVVSGSLAGIVRYAAGRGSDGVTSSTGEV-PEPPRW-----------

2nsgA

0.15

0.13

0.84

3.16

Env-PPAS

MTT----FHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETPM----YVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTAQGRVPASETLWMRSREVWIHAVDLGAVATFGDIP--------EVILRTLAAEITQKWTSQGAGEG----LVLLDEPSSTRYPA------------APGQDEVVVSGSLAGIVRYAAGRGS-----DGVTSSTGE-----VPEPPRW--

2nsgA1

0.14

0.08

0.55

0.97

MUSTER

MTT----FHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETPM----YVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTAQGRVPASETLWMRSREVWIHAVDLG---AVA---------------------------------------------------------------------------------------------------------------------

2nsgA1

0.13

0.07

0.55

1.06

dPPAS

----MTTFHDLPLEERLTLARLGTSHYSRQLSLVDNAEFGEHSLLEGWTRSHLIAAVAYNAIALCNLMHWANTGEETP----MYVSPEARNEEIAYGSTLNPDALRNLHEHSVARLDVAWRETSEDAWSHEVLTAQGRTPASETLWMRSREVWIHAVDLGAVA------------------------------------------------------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-0.78

Estimated TM-score = 0.61±0.14

Estimated RMSD = 7.8±4.4Å

Download Model 2

C-score=-1.14

Download Model 3

C-score=-1.29

Download Model 4

C-score=-1.78

Download Model 5

C-score=-3.13

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2nsgA	0.762	2.30	0.134	0.845
2	4x8bA	0.480	5.70	0.073	0.739
3	2x2hA	0.455	6.23	0.052	0.774
4	3cf4A	0.454	5.45	0.086	0.685
5	5cofA	0.454	3.18	0.130	0.541
6	4n6cA	0.452	3.41	0.102	0.551
7	3i39X	0.449	5.46	0.053	0.682
8	5cogA	0.448	3.39	0.157	0.544
9	1rxqB	0.445	3.33	0.138	0.537
10	3i04A	0.444	5.58	0.071	0.689

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.53	6	2nsfA	ZN	Rep, Mult	56,151,155
2	0.06	1	3rf3A	III	Rep, Mult	98,100,106
3	0.06	1	3cf4A	SF4	Rep, Mult	34,35,39,40,41,42,51,54,161

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1ogyI	0.435	6.41	0.059	0.774	1.7.99.4	155
2	0.060	1thgA	0.389	6.66	0.062	0.707	3.1.1.3	252
3	0.060	1jqoA	0.390	6.10	0.069	0.643	4.1.1.31	NA
4	0.060	1eg7A	0.390	5.82	0.066	0.629	6.3.4.3	NA
5	0.060	3ma8A	0.390	5.93	0.050	0.636	2.7.1.40	NA
6	0.060	1jqkF	0.440	5.44	0.062	0.675	1.2.99.2	NA
7	0.060	1c7sA	0.392	5.85	0.052	0.629	3.2.1.52	NA
8	0.060	3l4uA	0.391	6.80	0.042	0.721	3.2.1.20,3.2.1.3	NA
9	0.060	2jkpB	0.418	5.82	0.032	0.678	3.2.1.20	NA
10	0.060	1aqfE	0.374	6.48	0.053	0.664	2.7.1.40	NA
11	0.060	1u2zC	0.414	6.22	0.067	0.710	2.1.1.43	29
12	0.060	2nyaA	0.435	6.08	0.045	0.724	1.7.99.4	NA
13	0.060	2vrkA	0.389	6.43	0.042	0.664	3.2.1.55	174
14	0.060	1kqfA	0.408	6.25	0.043	0.689	1.2.1.2	NA
15	0.060	3h6oC	0.359	6.54	0.086	0.636	2.7.1.40	NA
16	0.060	2v45A	0.387	6.38	0.038	0.685	1.7.99.4	NA
17	0.060	3cskA	0.392	6.85	0.064	0.710	3.4.14.4	NA
18	0.060	3ecqB	0.410	6.26	0.064	0.700	3.2.1.97	149
19	0.060	2sqcA	0.395	5.66	0.050	0.625	5.4.99.17	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.43	0.761	2.31	0.13	0.84	2nsfA	GO:0046872
1	0.07	0.480	5.70	0.07	0.74	4x8bA	GO:0004497 GO:0005506 GO:0016491 GO:0016705 GO:0046872 GO:0052699 GO:0055114
2	0.07	0.412	6.25	0.05	0.70	5f7sB	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246
3	0.06	0.396	6.25	0.07	0.68	4kwuA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246 GO:0046872
4	0.06	0.385	6.72	0.06	0.70	5djwA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246
5	0.06	0.454	6.22	0.06	0.77	4amwA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0016829 GO:0030246 GO:0047457
6	0.06	0.391	6.80	0.04	0.72	3l4uA	GO:0000023 GO:0003824 GO:0004339 GO:0004553 GO:0004558 GO:0005886 GO:0005975 GO:0005983 GO:0008152 GO:0016020 GO:0016021 GO:0016160 GO:0016324 GO:0016787 GO:0016798 GO:0030246 GO:0032450 GO:0044245 GO:0070062
7	0.06	0.436	6.44	0.04	0.76	3wemA	GO:0000023 GO:0003824 GO:0004553 GO:0004558 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246 GO:0032450
8	0.06	0.427	6.47	0.06	0.74	5aedA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246
9	0.06	0.382	6.51	0.03	0.68	2g3mF	GO:0000023 GO:0003824 GO:0004553 GO:0004558 GO:0005737 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246 GO:0032450
10	0.06	0.406	6.53	0.02	0.72	3nsxA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246
11	0.06	0.379	6.92	0.04	0.72	4xpqA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246
12	0.06	0.440	6.34	0.05	0.76	2f2hA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246 GO:0042802 GO:0061634 GO:0080176
13	0.06	0.404	6.84	0.06	0.75	2xvgA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246
14	0.06	0.409	6.49	0.05	0.71	5f7cC	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246
15	0.06	0.411	6.48	0.05	0.71	5f7cA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246
16	0.06	0.440	6.56	0.06	0.78	3tonA	GO:0000023 GO:0003824 GO:0004339 GO:0004553 GO:0004558 GO:0005886 GO:0005975 GO:0005983 GO:0008152 GO:0016020 GO:0016021 GO:0016160 GO:0016324 GO:0016787 GO:0016798 GO:0030246 GO:0032450 GO:0044245 GO:0070062
17	0.06	0.376	6.40	0.05	0.66	4ba0A	GO:0003824 GO:0004553 GO:0005975 GO:0016740 GO:0016757 GO:0030246 GO:0033825
18	0.06	0.432	6.61	0.06	0.77	5dkyA	GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246

Consensus prediction of GO terms

Molecular Function	GO:0046872	GO:0016798
GO-Score	0.50	0.36
Biological Processes	GO:0044238
GO-Score	0.31
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.