I-TASSER results

I-TASSER results for job id Rv2734

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (284 residues)
MSDRSAIEWTGATWNPVTGCDRVSPGCDHCYAMTLAKRLKAMGSDKYQTDGDPRTSGPGF
GVTIHPRSLDEPFRWRSPRTVFVNSMADLFHARVALWFIREVFEVMRATPQHTYQILTKR
SLRLRRLAHKLEWPSNVWMGVSVENVDAFRRIEDLRQVPAAVRFLSCEPLLGPLDGINLG
SIDWVIAGGESGPNFRPIDPQWVRHIRDTCTAADVPFFFKQWGGRTPKAFGRELDGRCWD
EMPLIEIRNPDPRTTSRVHADPMLATAPTESAQRSNPGQLVRQR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSDRSAIEWTGATWNPVTGCDRVSPGCDHCYAMTLAKRLKAMGSDKYQTDGDPRTSGPGFGVTIHPRSLDEPFRWRSPRTVFVNSMADLFHARVALWFIREVFEVMRATPQHTYQILTKRSLRLRRLAHKLEWPSNVWMGVSVENVDAFRRIEDLRQVPAAVRFLSCEPLLGPLDGINLGSIDWVIAGGESGPNFRPIDPQWVRHIRDTCTAADVPFFFKQWGGRTPKAFGRELDGRCWDEMPLIEIRNPDPRTTSRVHADPMLATAPTESAQRSNPGQLVRQR
Prediction	CCCCCCCCCCCCEHCCEHCCCHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEHCCCCCCCCCHHHHHCHHHCCCCCEEEHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHCCHHHHHHHHHHCCCCCCEEEEEEEHCCCHHHHHHHHHHCCCCEEEEEEHCCCCCCCCCCCCCCCEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHCCCCCCCCCCCCCCCCHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	98888887667654765577456865566579999999887777777777656665788667658788755676678968998578887777799999999999999879985999969989999986557887868999998677677899999869986689999678777788888777689986778898888899999999999999899889976688766667755577434457754446677877665556776655444445766655444789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSDRSAIEWTGATWNPVTGCDRVSPGCDHCYAMTLAKRLKAMGSDKYQTDGDPRTSGPGFGVTIHPRSLDEPFRWRSPRTVFVNSMADLFHARVALWFIREVFEVMRATPQHTYQILTKRSLRLRRLAHKLEWPSNVWMGVSVENVDAFRRIEDLRQVPAAVRFLSCEPLLGPLDGINLGSIDWVIAGGESGPNFRPIDPQWVRHIRDTCTAADVPFFFKQWGGRTPKAFGRELDGRCWDEMPLIEIRNPDPRTTSRVHADPMLATAPTESAQRSNPGQLVRQR
Prediction	85651614103100000200232062041000110041153244442445334324464141213462264115265442000000000025423450043005003714502000002216203500663712400000000224512400420360504110000000043064250520100000023156324143500430151057370200000105314554344134431441243414534544444423444423221441344203332558
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCEHCCEHCCCHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEHCCCCCCCCCHHHHHCHHHCCCCCEEEHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHCCHHHHHHHHHHCCCCCCEEEEEEEHCCCHHHHHHHHHHCCCCEEEEEEHCCCCCCCCCCCCCCCEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEHCCCCCCCCCCCCCCCCHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
MSDRSAIEWTGATWNPVTGCDRVSPGCDHCYAMTLAKRLKAMGSDKYQTDGDPRTSGPGFGVTIHPRSLDEPFRWRSPRTVFVNSMADLFHARVALWFIREVFEVMRATPQHTYQILTKRSLRLRRLAHKLEWPSNVWMGVSVENVDAFRRIEDLRQVPAAVRFLSCEPLLGPLDGINLGSIDWVIAGGESGPNFRPIDPQWVRHIRDTCTAADVPFFFKQWGGRTPKAFGRELDGRCWDEMPLIEIRNPDPRTTSRVHADPMLATAPTESAQRSNPGQLVRQR

4fhcA

0.15

0.11

0.77

1.36

wPPAS

LKFDSSKPSAEYAIPLATGCMG---HCHYCYLQTT-----------LGSKPYIRVYV---NLDDIFAQAQKYINERAPERFEAACTSDIV-GIDHLTSLKKAIEFIGATDYGRLRFVTKYEDHLLDA----RHNGKTRFRFSINSRSFDGRLAAARKV-GYKLGFVVAPIYRHEGWER----GY---------------FELFQELARQLEGMDLSDLTFELIQRFTKPAKRVIEQR----YPKTRLD--------------------LDETKRKKWGRYGIGK

4fhcA

0.15

0.11

0.70

1.44

wdPPAS

LKFDSSKPSAEYAIPLATGCMG---HCHYCYLQTT-----------LGSKPYIRVYVN---LDDIFAQAQKYINERAPERFEAACTSDIV-GIDHLTSLKKAIEFIGATDYGRLRFVTKYEHVDHLLD--ARHNGKTRFRFSINSRSFDGRLAAARKV-GYKLGFVVAPIYRHEGQLEGMDLSDLTFEGRYGIGKYVYRDEEAKELEDTMRRY-FPQYFT----------------------------------------------------------------

4fhcA1

0.15

0.07

0.46

2.35

dPPAS2

---------AEYAIPLATGCMG---HCHYCYLQTT-----------LGSKPYIRVYV---NLDDIFAQAQKYINERAPERFEAACTSDIVGIDHLTHSLKKAIEFIGATDYGRLRFVTKYEH--VDHLLDARHNGKTRFRFSINSRSFDGRLAAARKV------------------------------------------------------------------------------------------------------------------------------

4fhcA1

0.16

0.08

0.49

1.30

wdPPAS

LKFDSSKPSAEYAIPLATGCMG---HCHYCYLQTT-----------LGSKPYIRVYVN---LDDIFAQAQKYINERAPERFEAACTSDIV-GIDHLTSLKKAIEFIGATDYGRLRFVTKYEHV--DHLLDARHNGKTRFRFSINSRSFDGRLAAARKV-G----------------------------------------------------------------------------------------------------------------------------

4fhcA1

0.18

0.09

0.50

1.07

wPPAS

LKFDSSKPSAEYAIPLATGCMG---HCHYCYLQTT-----------LGSKPYIRVYV---NLDDIFAQAQKYINERAPERFEAACTSDIV-GIDHLTSLKKAIEFIGATDYGRLRFVTKYEDHLLDA----RHNGKTRFRFSINSRYFDGRLAAARKV--------AGK-LG----------------------------------------------------------------------------------------------------------------

4rwzA1

0.15

0.04

0.28

1.79

dPPAS2

----------------------------------------------------MIVQLGKASVTWTRADLEAKLAGHARVLIDVGTGDG-----------RFVYRSAGAHPDTYCIGVDPAGERMREVSWRAGGRPNALFVVA----------------------------------------------------------------------------------------------------------------------------------------------

4fhcA

0.13

0.09

0.68

1.70

dPPAS2

LKFDSSKPSAEYAIPLATGCMG---HCHYCYLQTT-----------LGSKPYIRVYVN---LDDIFAQAQKYINERAPERFEAACTSDIVGIDHLTHSLKKAIEFIGATDYGRLRFVTKYEH--VDHLLDARHNGKTRFRFSINSRSFDGRLAAARKVAGAKLGFVVAPIYRHEGQLEGMDLSDLTFEGRYGIGRDEEAKELEDTMRRYIEQF-----------------------------------------------------------------------

2mr6A

0.10

0.05

0.44

1.42

dPPAS2

----------------------------------MERIAEELRKEVQKHDPNVKTVPTKDKEKV-KEEIEKARKQGRPIVVFVRGGD--------DERAKDIAEYAQKEGLRVIVIIVSQDEEALRKGYEDKKKKGYDVYTSRNEDEAKKKLKEALEK------------SGSLEHHHHHH-------------------------------------------------------------------------------------------------------

3j38h

0.05

0.01

0.15

1.38

dPPAS2

------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YGNKESNGEFGNELPQRQFNNRDNRGPPRVRTGEKFGKREFKEE

4fhcA

0.12

0.11

0.88

0.81

MUSTER

KFDSSK-PSAEYAIPLATGCMG---HCHYCYLQTT-----------LGSKPYIRVYV---NLDDIFAQAQKYINERAPERFEAACTSDIVGIDHLTHSLKKAIEFIGATDYGRLRFVTKYE--HVDHLLDARHNGKTRFRFSINSRYVINHFEP-GTSSFDGR-------LAAARKVAGAGYKGFVVAIYRHEGWERGYFELFQELARQLEGMDLTFELIQ--HRFTKPAKRVIEQR----YPKTRLDLDETKRKYKWGRYGIGKYVEAKEL-EDTMRRYIEQF

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.33

Estimated TM-score = 0.44±0.14

Estimated RMSD = 11.5±4.5Å

Download Model 2

C-score=-3.40

Download Model 3

C-score=-3.46

Download Model 4

C-score=-5.00

Download Model 5

C-score=-4.82

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4fhcA	0.712	3.21	0.117	0.842
2	3c8fA	0.578	4.00	0.098	0.750
3	1itxA	0.577	4.65	0.058	0.803
4	3b9eA2	0.573	4.95	0.048	0.820
5	1x6lA	0.568	4.97	0.045	0.806
6	5dezA2	0.567	5.00	0.057	0.813
7	1kfwA	0.566	4.93	0.057	0.803
8	3qokA	0.564	5.15	0.049	0.824
9	3oa5A	0.563	4.76	0.091	0.796
10	4dwsA	0.563	4.76	0.073	0.792

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.20	9	4rh1A	SF4	Rep, Mult	20,27,30,32,119
2	0.16	7	4fhcA	EEM	Rep, Mult	29,30,31,32,84,87,88,117,119,142,166,168,169,224,226
3	0.02	1	3as0A	SAU	Rep, Mult	169,170,188,189,190,192
4	0.02	1	2xsjC	UUU	Rep, Mult	26,27
5	0.02	1	1tgvB	5UD	Rep, Mult	90,91
6	0.02	1	3as2A	POY	Rep, Mult	140,142,145,153,154,155,156
7	0.02	1	1h4pA	UUU	Rep, Mult	153,205,208,209,212
8	0.02	1	3fidA	ZN	Rep, Mult	88,118
9	0.02	1	2pc8A	BGC	Rep, Mult	107,112,113,138

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1zjaA	0.530	5.48	0.072	0.810	5.4.99.11	NA
2	0.060	1wcgA	0.531	5.34	0.053	0.799	3.2.1.147	NA
3	0.060	1fhlA	0.531	4.43	0.060	0.736	3.2.1.89	NA
4	0.060	1m53A	0.541	5.27	0.064	0.813	5.4.99.12	NA
5	0.060	2bhzA	0.536	5.12	0.051	0.799	3.2.1.141	NA
6	0.060	1bgaA	0.526	5.33	0.057	0.782	3.2.1.21	70
7	0.060	1g5aA	0.540	5.22	0.064	0.806	2.4.1.4	NA
8	0.060	2zicA	0.530	5.30	0.051	0.803	3.2.1.70	NA
9	0.060	1fa2A	0.530	4.48	0.039	0.718	3.2.1.2	NA
10	0.060	1e1eB	0.525	5.00	0.051	0.757	3.2.1.21	NA
11	0.060	2vr5A	0.530	5.38	0.045	0.799	3.2.1.-	NA
12	0.060	1kfwA	0.566	4.93	0.057	0.803	3.2.1.14	NA
13	0.060	3cb8A	0.585	3.86	0.075	0.750	1.97.1.4	NA
14	0.060	1v03A	0.533	5.29	0.047	0.792	3.2.1.21	NA
15	0.060	1qbaA	0.541	4.59	0.075	0.750	3.2.1.52	NA
16	0.060	1edqA	0.567	4.90	0.037	0.803	3.2.1.14	NA
17	0.060	2zidA	0.530	5.25	0.056	0.799	3.2.1.70	144,247
18	0.060	3gbdA	0.540	5.31	0.051	0.813	5.4.99.11	NA
19	0.060	1c7sA	0.542	4.60	0.080	0.753	3.2.1.52	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.18	0.712	3.21	0.12	0.84	4fhcA	GO:0003913 GO:0016829 GO:0046872 GO:0051536 GO:0051539
1	0.13	0.578	4.00	0.10	0.75	3c8fA	GO:0003824 GO:0005737 GO:0005829 GO:0005975 GO:0006006 GO:0006974 GO:0016491 GO:0018307 GO:0043365 GO:0046872 GO:0051536 GO:0051539 GO:0055114
2	0.07	0.573	4.95	0.05	0.82	3b9eA	GO:0004553 GO:0004568 GO:0005576 GO:0005975 GO:0006032 GO:0008152 GO:0016787 GO:0016798 GO:0030246
3	0.07	0.567	4.90	0.04	0.80	1edqA	GO:0004553 GO:0004568 GO:0005975 GO:0006032 GO:0008152 GO:0016787 GO:0016798
4	0.07	0.569	5.12	0.06	0.83	3qokA	GO:0004553 GO:0004568 GO:0005975 GO:0006032 GO:0008152 GO:0016787 GO:0016798
5	0.07	0.577	4.65	0.06	0.80	1itxA	GO:0000272 GO:0004553 GO:0004568 GO:0005576 GO:0005975 GO:0006032 GO:0008152 GO:0016787 GO:0016798 GO:0030246
6	0.07	0.563	4.76	0.09	0.80	4a5qA	GO:0004553 GO:0004568 GO:0005975 GO:0006032
7	0.07	0.566	4.93	0.06	0.80	1kfwA	GO:0004553 GO:0004568 GO:0005576 GO:0005975 GO:0006032 GO:0008152 GO:0016787 GO:0016798 GO:0030246
8	0.07	0.566	5.11	0.05	0.82	4lgxA	GO:0004553 GO:0004568 GO:0005975 GO:0006032 GO:0008152 GO:0016787 GO:0016798
9	0.07	0.544	4.53	0.05	0.75	1vf8A	GO:0000272 GO:0004553 GO:0004563 GO:0004568 GO:0005576 GO:0005634 GO:0005635 GO:0005737 GO:0005783 GO:0005975 GO:0006032 GO:0006954 GO:0008061 GO:0008152 GO:0016023 GO:0016787 GO:0016798 GO:0030246 GO:0048237
10	0.07	0.548	4.59	0.07	0.76	3w4rA	GO:0004553 GO:0004568 GO:0005576 GO:0005975 GO:0006030 GO:0006032 GO:0008061 GO:0008152 GO:0016787 GO:0016798
11	0.07	0.528	4.89	0.05	0.75	4w5uB	GO:0004553 GO:0004568 GO:0005975 GO:0006032
12	0.07	0.542	4.58	0.07	0.75	1hjvA	GO:0004553 GO:0004568 GO:0005201 GO:0005576 GO:0005578 GO:0005615 GO:0005737 GO:0005783 GO:0005975 GO:0006032 GO:0006915 GO:0006954 GO:0007250 GO:0008061 GO:0009612 GO:0010800 GO:0030246 GO:0030324 GO:0034612 GO:0045766 GO:0048471 GO:0051216 GO:0051897 GO:0070062 GO:0070374 GO:0070555 GO:0070741 GO:0071347 GO:0071356 GO:0072606
13	0.07	0.533	4.73	0.08	0.75	3aquA	GO:0004553 GO:0004568 GO:0005576 GO:0005618 GO:0005975 GO:0006032 GO:0008061 GO:0008843 GO:0009651 GO:0009737 GO:0009753 GO:0035885
14	0.07	0.542	4.53	0.09	0.74	2aosA	GO:0004553 GO:0004568 GO:0005576 GO:0005615 GO:0005737 GO:0005783 GO:0005975 GO:0006032 GO:0006915 GO:0006954 GO:0007250 GO:0008061 GO:0009612 GO:0010800 GO:0030246 GO:0030324 GO:0034612 GO:0045766 GO:0048471 GO:0051897 GO:0070374 GO:0070555 GO:0070741 GO:0071356 GO:0072606
15	0.07	0.539	4.78	0.04	0.76	1jndA	GO:0004553 GO:0004568 GO:0005576 GO:0005975 GO:0006032 GO:0007275 GO:0007444 GO:0008061 GO:0008084
16	0.07	0.541	4.56	0.08	0.74	3fxyA	GO:0000272 GO:0002376 GO:0002532 GO:0003796 GO:0004553 GO:0004568 GO:0005576 GO:0005615 GO:0005737 GO:0005975 GO:0006030 GO:0006032 GO:0006037 GO:0006915 GO:0006954 GO:0006955 GO:0007586 GO:0008061 GO:0008152 GO:0009620 GO:0016787 GO:0016798 GO:0019900 GO:0030246 GO:0090197
17	0.07	0.540	4.59	0.04	0.75	4mnmA	GO:0004553 GO:0004568 GO:0005975 GO:0006032
18	0.07	0.533	4.67	0.09	0.75	4wiwD	GO:0004553 GO:0004568 GO:0005975 GO:0006032 GO:0016787 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0043169	GO:0051540	GO:0004553	GO:0016830
GO-Score	0.56	0.56	0.37	0.35
Biological Processes	GO:0044238	GO:1901072	GO:0006030	GO:0006026
GO-Score	0.50	0.37	0.37	0.37
Cellular Component	GO:0005829	GO:0005576
GO-Score	0.13	0.07

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.