I-TASSER results

I-TASSER results for job id Rv2716

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (228 residues)
MAIEVSVLRVFTDSDGNFGNPLGVINASKVEHRDRQQLAAQSGYSETIFVDLPSPGSTTA
HATIHTPRTEIPFAGHPTVGASWWLRERGTPINTLQVPAGIVQVSYHGDLTAISARSEWA
PEFAIHDLDSLDALAAADPADFPDDIAHYLWTWTDRSAGSLRARMFAANLGVTEDEATGA
AAIRITDYLSRDLTITQGKGSLIHTTWSPEGWVRVAGRVVSDGVAQLD

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MAIEVSVLRVFTDSDGNFGNPLGVINASKVEHRDRQQLAAQSGYSETIFVDLPSPGSTTAHATIHTPRTEIPFAGHPTVGASWWLRERGTPINTLQVPAGIVQVSYHGDLTAISARSEWAPEFAIHDLDSLDALAAADPADFPDDIAHYLWTWTDRSAGSLRARMFAANLGVTEDEATGAAAIRITDYLSRDLTITQGKGSLIHTTWSPEGWVRVAGRVVSDGVAQLD
Prediction	CCEEEEEEEEHCCCCCCCCCEEEEHCCCCCCHHHHHHHHHHHCCCEEEEEHCCCCCCCCEEEEEHCCCCCHCCCCCHHHHHHHHHHHHCCCCEEEEHCCEEEEEEEHCCEEEEEHCCCCCCCEEEEHCCCHHHHHCCCHHHHHCCCEEEEEHCCCCCCCEEEEEEHCCCCCCCCCCCCCCHCHHHHHHHHHHHCCHCCCCCEEEEEEHCCCEEEEEEEEEEEEEEEHC
Conf.Score	967689998657999999986899759999989999999998998599998689999858999977886446888757899999999899866999787789999968756788657889875898768988998599577656766899985899997799987578889989876675545799999998684337895899999589859998899999999989
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MAIEVSVLRVFTDSDGNFGNPLGVINASKVEHRDRQQLAAQSGYSETIFVDLPSPGSTTAHATIHTPRTEIPFAGHPTVGASWWLRERGTPINTLQVPAGIVQVSYHGDLTAISARSEWAPEFAIHDLDSLDALAAADPADFPDDIAHYLWTWTDRSAGSLRARMFAANLGVTEDEATGAAAIRITDYLSRDLTITQGKGSLIHTTWSPEGWVRVAGRVVSDGVAQLD
Prediction	672402101000454362000000024760437202400562524000000216456230201010244303201100000000014445453204051140303244331102022333232002315314303414144125333202022244442211000102433030000001000000010254141523330203031477230100030033041418
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCEEEEEEEEHCCCCCCCCCEEEEHCCCCCCHHHHHHHHHHHCCCEEEEEHCCCCCCCCEEEEEHCCCCCHCCCCCHHHHHHHHHHHHCCCCEEEEHCCEEEEEEEHCCEEEEEHCCCCCCCEEEEHCCCHHHHHCCCHHHHHCCCEEEEEHCCCCCCCEEEEEEHCCCCCCCCCCCCCCHCHHHHHHHHHHHCCHCCCCCEEEEEEHCCCEEEEEEEEEEEEEEEHC
MAIEVSVLRVFTDSDGNFGNPLGVINASKVEHRDRQQLAAQSGYSETIFVDLPSPGSTTAHATIHTPRTEIPFAGHPTVGASWWLRERGTPINTLQVPAGIVQVSYHGDLTAISARSEWAPEFAIHDLDSLDALAAADPADFPDDIAHYLWTWTDRSAGSLRARMFAANLGVTEDEATGAAAIRITDYLSRDLTITQGKGSLIHTTWSPEGWVRVAGRVVSDGVAQLD

1s7jA

0.18

0.17

0.97

2.79

MUSTER

MSYPYYIVDAFAEEVF-KGNPAAVYVLKWLPEAVMQNIAIENNLSETAFTVK---EGQSYALRWFTPEREIDLCGHATLATAFVLFNYYEETLHFTSQSGPLAVTKKEEYYYLDFPYILPGRDLFFVLKDEETVAKITPDFSA-DLGVGVIVTASGDSVDFVSRTFFPKLRINEDPVCGSAHANLIPYWGKRLNQTTPRGGFLTCEVKEN-RVIIGGTAKLFAKGEAY

1s7jA

0.17

0.98

3.18

dPPAS

MSYPYYIVDAFAEEVF-KGNPAAVYVEKWLPEAVMQNIAIENNLSETAFTVK---EGQSYALRWFTPEREIDLCGHATLATAFVLFNYYSVALHFTSQSGPLAVTKKEEYYYLDFPYILPGRDLFFVLKDEETVAKITPDFSALDLGVGVIVTASGDSVDFVSRTFFPKLRINEDPVCGSAHANLIPYWGKRLNQTTPRGGFLTCEVKE-NRVIIGGTAKLFAKGEAY

1s7jA

0.17

0.97

4.59

wdPPAS

MSYPYYIVDAFAEEVFK-GNPAAVYVEKWLPEAVMQNIAIENNLSETAFTVKE---GQSYALRWFTPEREIDLCGHATLATAFVLFNYYEETLHFTSQSGPLAVTKKEEYYYLDFPYILLGRDLFFVLKDEETVAKITPDFSA-DLGVGVIVTASGDSVDFVSRTFFPKLRINEDPVCGSAHANLIPYWGKRLNQ-SPRGGFLTCEVKEN-RVIIGGTAKLFAKGEAY

1s7jA

0.17

0.97

3.25

wMUSTER

-SYPYYIVDAFAEEVF-KGNPAAVYVEKWLPEAVMQNIAIENNLSETAFTVK---EGQSYALRWFTPEREIDLCGHATLATAFVLFNYYEETLHFTSQSGPLAVTKKEEYYYLDFPYILPGRDLFFVLKDEETVAKITPDFSA-DLGVGVIVTASGDSVDFVSRTFFPKLRINEDPVCGSAHANLIPYWGKRLNQTTPRGGFLTCEVKEN-RVIIGGTAKLFAKGEAY

1s7jA

0.18

0.97

4.99

wPPAS

MSYPYYIVDAFAEEVF-KGNPAAVYVEKWLPEAVMQNIAIENNLSETAFTVKEG---QSYALRWFTPEREIDLCGHATLATAFVLFNYYEETLHFTSQSGPLAVTKKEEYYYLDFPYILLGRDLFFVLKDEETVAKITPDFSA-DLGVGVIVTASGDSVDFVSRTFFPKLRINEDPVCGSAHANLIPYWGKRLNQTTPRGGFLTCEVK-ENRVIIGGTAKLFAKGEAY

1s7jA

0.17

0.98

6.50

dPPAS2

MSYPYYIVDAFAEEVF-KGNPAAVYVEKWLPEAVMQNIAIENNLSETAFTVK---EGQSYALRWFTPEREIDLCGHATLATAFVLFNYYSVALHFTSQSGPLAVTKKEEYYYLDFPYILPGRDLFFVLKDEETVAKITPDFSALDLGVGVIVTASGDSVDFVSRTFFPKLRINEDPVCGSAHANLIPYWGKRLNQTTPRGGFLTCEVKE-NRVIIGGTAKLFAKGEAY

1s7jA

0.18

0.97

4.07

PPAS

MSYPYYIVDAFAEEVF-KGNPAAVYVEKWLPEAVMQNIAIENNLSETAFTVKEG---QSYALRWFTPEREIDLCGHATLATAFVLFNYYSVALHFTSQSGPLAVTKKEEYYYLDFPYILPGRDLFFVLKDEETVAKITPDFSA-DLGVGVIVTASGDSVDFVSRTFFPKLRINEDPVCGSAHANLIPYWGKRLNQTTPRGGFLTCEVK-ENRVIIGGTAKLFAKGEAY

1s7jA

0.18

0.98

4.80

Env-PPAS

MSYPYYIVDAFAEEVF-KGNPAAVYVEKWLPEAVMQNIAIENNLSETAFTVKEG---QSYALRWFTPEREIDLCGHATLATAFVLFNYYSETLHFTSQSGPLAVTKKEEYYYLDFPYILPGRDLFFVLKDEETVAKITPDFSALDLGVGVIVTASGDSVDFVSRTFFPKLRINEDPVCGSAHANLIPYWGKRLNQTTPRGGFLTCEVK-ENRVIIGGTAKLFAKGEAY

4dunA

0.21

0.97

2.59

MUSTER

-AMEYYIVDSFATKLF-KGNPAGVCVDRRIPLELMQKIAEENNLPETAFVV---KGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNIDFFTQSGKLEVTRNGNLYEMIFPEIMPSRDLILLLNSEQEVINYKPNYAQLRKWLGIIITAQGSNTDFVSRYFCPELD-SEDPVTGSSHCNLIPYWSEKLGKHKNRGGIIQCEVLKDNTVKISGEAVLFMQGTIK

4dunA

0.21

0.97

2.92

dPPAS

-AMEYYIVDSFATKLF-KGNPAGVCVDRRIPLELMQKIAEENNLPETAFVV---KGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNIDFFTQSGKLEVTRNGNLYEMIFPEIMPSRDLILLLNSEQEVINYKPNYAQLTDWLGIIITAQGSNTDFVSRYFCPELD-SEDPVTGSSHCNLIPYWSEKLGKHKNRGGIIQCEVLKDNTVKISGEAVLFMQGTIK

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 1 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.49

Estimated TM-score = 0.92±0.06

Estimated RMSD = 2.7±2.0Å

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1s7jA	0.949	1.05	0.170	0.978
2	4dunA	0.935	1.21	0.198	0.974
3	1qyaB	0.884	2.17	0.218	0.987
4	1ym5A	0.871	2.20	0.236	0.987
5	1u1wB	0.860	2.38	0.244	0.987
6	3ednA	0.853	2.14	0.224	0.961
7	4jbdA	0.824	2.61	0.137	0.961
8	1u0kA	0.817	2.65	0.237	0.961
9	4juuA	0.808	2.73	0.189	0.952
10	5ha4A	0.799	2.68	0.171	0.947

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.55	14	1u1wA	3HA	Rep, Mult	45,46,71,74,75,76,77,165,170,175,177,178,179,180
2	0.03	1	1t6kA	SO4	Rep, Mult	122,148
3	0.03	1	4juuA	PO4	Rep, Mult	76,175,177,178,179
4	0.03	1	1qya0	III	Rep, Mult	5,7,9,15,16,21,38,40,41,42,221,222,223,224,225,226,227
5	0.02	1	3eubS	FES	Rep, Mult	12,13,14,16,17,18,19,222
6	0.02	1	1xub0	III	Rep, Mult	32,63,70,98,100
7	0.02	1	1lm1A	F3S	Rep, Mult	36,37,38,39,42,43,44,47,65,66

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.153	1w62A	0.764	3.15	0.138	0.947	5.1.1.4	NA
2	0.126	3fveA	0.783	2.92	0.130	0.943	5.1.1.7	NA
3	0.109	2otnA	0.797	2.69	0.134	0.947	5.1.1.7	NA
4	0.093	3ejxD	0.773	3.06	0.129	0.947	5.1.1.7	NA
5	0.067	1tm0A	0.686	3.24	0.086	0.864	5.1.1.4	64,67
6	0.060	1qo9A	0.415	5.62	0.082	0.689	3.1.1.7	NA
7	0.060	2cf2C	0.433	5.06	0.058	0.667	2.3.1.85	65,75
8	0.060	1ofdA	0.374	5.55	0.055	0.605	1.4.7.1	NA
9	0.060	1rm6A	0.423	6.23	0.060	0.789	1.3.99.20	NA
10	0.060	2azpA	0.785	2.95	0.134	0.952	5.1.1.8	21
11	0.060	2pflA	0.408	5.00	0.018	0.636	2.3.1.54	NA
12	0.060	1l5jA	0.420	5.59	0.052	0.724	4.2.1.3	39
13	0.060	2e1qA	0.425	6.04	0.040	0.763	1.17.3.2,1.17.1.4	NA
14	0.060	1bwzA	0.762	2.99	0.104	0.930	5.1.1.7	NA
15	0.060	2ckjA	0.310	6.08	0.030	0.553	1.17.1.4,1.17.3.2	NA
16	0.060	1eveA	0.415	5.57	0.059	0.689	3.1.1.7	NA
17	0.060	3b9jI	0.226	5.80	0.034	0.386	1.17.1.4,1.17.3.2	134,200
18	0.060	1ea0A	0.377	5.47	0.060	0.597	1.4.1.13	79
19	0.060	2veoB	0.399	5.89	0.050	0.702	3.1.1.3	NA
20	0.060	2pm8A	0.418	5.66	0.074	0.693	3.1.1.8	NA
21	0.060	2vdcA	0.425	5.80	0.076	0.737	1.4.1.13	NA
22	0.060	1ffuB	0.416	6.28	0.094	0.772	1.2.99.2	NA
23	0.060	1ygaA	0.409	5.60	0.050	0.697	5.1.3.3	61
24	0.060	1y791	0.416	5.40	0.073	0.684	3.4.15.5	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.47	0.860	2.38	0.24	0.99	1u1wB	GO:0002047 GO:0003824 GO:0009058 GO:0009405 GO:0016853 GO:0017000
1	0.43	0.935	1.21	0.20	0.97	4dunA	GO:0003824 GO:0009058
2	0.42	0.886	2.02	0.22	0.99	3ednA	GO:0003824 GO:0009058
3	0.42	0.949	1.05	0.17	0.98	1s7jA	GO:0003824 GO:0009058
4	0.40	0.899	2.01	0.23	0.99	1sdjA	GO:0003824 GO:0009058 GO:0016853
5	0.33	0.823	2.60	0.24	0.96	1u0kA	GO:0003824 GO:0009058
6	0.27	0.822	2.53	0.15	0.96	4jd7A	GO:0016853 GO:0047580
7	0.27	0.871	2.20	0.24	0.99	1ym5A	GO:0003824 GO:0009058 GO:0016853 GO:0030968
8	0.23	0.785	2.98	0.14	0.95	4k7xA	GO:0016853 GO:0047580
9	0.22	0.808	2.73	0.19	0.95	4juuA	GO:0016853 GO:0047580
10	0.13	0.802	2.65	0.17	0.95	5ha4B	GO:0005737 GO:0008652 GO:0008837 GO:0009085 GO:0009089 GO:0016853
11	0.12	0.783	2.99	0.12	0.95	4j9wA	GO:0016853 GO:0047580
12	0.11	0.819	2.53	0.17	0.95	4jciA	GO:0016853 GO:0047580
13	0.10	0.785	2.95	0.13	0.95	2azpA	GO:0016853 GO:0047580
14	0.07	0.764	3.15	0.14	0.95	1w62A	GO:0005576 GO:0005737 GO:0007275 GO:0016020 GO:0016853 GO:0018112
15	0.06	0.346	6.13	0.05	0.63	3r0xA	GO:0003941 GO:0005737 GO:0006520 GO:0006563 GO:0008721 GO:0016829 GO:0016836 GO:0030170 GO:0030378 GO:0036088 GO:0046416 GO:0070179
16	0.06	0.304	6.06	0.04	0.53	3a1nA	GO:0000166 GO:0003824 GO:0050662
17	0.06	0.267	6.50	0.03	0.51	5fmgH	GO:0000502 GO:0004298 GO:0005839 GO:0016787 GO:0051603
18	0.06	0.265	5.95	0.03	0.47	2ax4A	GO:0000103 GO:0000166 GO:0001501 GO:0003824 GO:0004020 GO:0004781 GO:0005524 GO:0005829 GO:0007596 GO:0008152 GO:0016301 GO:0016310 GO:0016740 GO:0016779 GO:0050428 GO:0060348
19	0.06	0.269	5.09	0.06	0.41	5i01A	GO:0005737 GO:0005975 GO:0008270 GO:0008968 GO:0016853 GO:0030246 GO:0046872 GO:2001061
20	0.06	0.247	5.69	0.04	0.42	3fc3A	GO:0046872
21	0.06	0.294	6.11	0.05	0.53	4nhyA	GO:0005506 GO:0005634 GO:0005737 GO:0006449 GO:0008283 GO:0010494 GO:0016491 GO:0016705 GO:0016706 GO:0018126 GO:0019511 GO:0031418 GO:0031543 GO:0031544 GO:0034063 GO:0046872 GO:0051213 GO:0055114
22	0.06	0.244	5.82	0.01	0.41	1oi4A	GO:0005737 GO:0006979 GO:0009268 GO:0009408 GO:0009411 GO:0016787 GO:0016798 GO:0019172 GO:0019243 GO:0019249 GO:0042802
23	0.06	0.187	4.90	0.08	0.29	1e9mA	GO:0009055 GO:0046872 GO:0051536 GO:0051537 GO:0055114
24	0.06	0.186	4.94	0.08	0.29	2a3jA	GO:0000166 GO:0000398 GO:0003676 GO:0003723 GO:0005634 GO:0005654 GO:0005681 GO:0005685 GO:0005730 GO:0005737 GO:0006397 GO:0008380 GO:0017069 GO:0030529 GO:0030619 GO:0035614 GO:0044822
25	0.06	0.195	4.94	0.03	0.30	2p3hA	GO:0003824 GO:0016020 GO:0016021 GO:0016614 GO:0050660 GO:0055114
26	0.06	0.220	5.97	0.04	0.38	4glkA	GO:0003723 GO:0004518 GO:0004519 GO:0004521 GO:0005737 GO:0016787 GO:0051607 GO:0090305 GO:0090502

Consensus prediction of GO terms

Molecular Function	GO:0016853
GO-Score	0.69
Biological Processes	GO:0002047	GO:0009405
GO-Score	0.47	0.47
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.