I-TASSER results

I-TASSER results for job id Rv2689c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (405 residues)
MTRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEA
FEVIDPSPDRIGSLCSIAGADGAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEA
QPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLDCGQLPVGMLDGLVAADWPPE
AQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAH
RDAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAA
RAALVDLPQVEVVTGSVRRVLAVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIG
CEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVALLTRKV

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEAFEVIDPSPDRIGSLCSIAGADGAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEAQPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLDCGQLPVGMLDGLVAADWPPEAQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAHRDAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAARAALVDLPQVEVVTGSVRRVLAVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIGCEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVALLTRKV
Prediction	CCCCCCCCEEEEEEEEEHCCCCCEEEEHCCEEEEHCCCCCCEEEEEEEEEEHCCEEEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCEEEEEHCCCCCCEEHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHCCCCEEEEEHHHCCCCCCCCCEEEEEHCCCCEEEEHCCEEEEHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEHCCCCCHHHHHHHHHCCCEEEEEEHCCHHHHHHHHHHHHHCCCCEEEEHCCHHHHHHHHCCCCCEEEHCCCCCCCCHHHHHHHHHHCCCEEEEEHCCHHHHHHHHHHHHHHCCEEEEEEEHCCCCCCCCEEEEEEEEHCC
Conf.Score	988888866899999986589868999899999968999988999999997598689999988645887689999777888999875458999999999999999999998699987654677889988755689988688556667645778678656776689789999998865667779999956894676554444666655444567747884689998989998798766678899999999999998799998899957897789999998758468999976658999999999989997899986899999986999989998899999679999999998999799997899999999999999898899999865899986347899999869
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEAFEVIDPSPDRIGSLCSIAGADGAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEAQPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLDCGQLPVGMLDGLVAADWPPEAQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAHRDAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAARAALVDLPQVEVVTGSVRRVLAVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIGCEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVALLTRKV
Prediction	765566631503030440025020001166100003300120303020244355102020240054065115130410326400000002041730152025103500440272625421300204512110100101034521000123224310304402212410030003032322110000012433420222035245532422120022341020203604040204000002240033004201610735652100000010000000004204620001001343442143116437064030102302510473554000000000233014500520171515120000010000030032027343303301001000100200000043257
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCEEEEEEEEEHCCCCCEEEEHCCEEEEHCCCCCCEEEEEEEEEEHCCEEEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCEEEEEHCCCCCCEEHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHCCCCEEEEEHHHCCCCCCCCCEEEEEHCCCCEEEEHCCEEEEHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEHCCCCCHHHHHHHHHCCCEEEEEEHCCHHHHHHHHHHHHHCCCCEEEEHCCHHHHHHHHCCCCCEEEHCCCCCCCCHHHHHHHHHHCCCEEEEEHCCHHHHHHHHHHHHHHCCEEEEEEEHCCCCCCCCEEEEEEEEHCC
MTRAGDDAVNLTLVTGAPANGGSCVAHHEGRVVFVRYALPGERVRARVTAQRGSYWHAEAFEVIDPSPDRIGSLCSIAGADGAGCCDLAFAAPEAARTLKAQVVANQLERLGRHSWQGEAQPLSDAGPTGWRIRVRLDVGADRRPGFHRYHSGELVTDLDCGQLPVGMLDGLVAADWPPEAQLYVALDDDGERHVVCSVRQGPRNRTRTVTNVVEGAYHAHQRVHRRSWRVPVTAFWQAHRDAAAVYSDLIADWAQPAPGMTAWDLYGGAGVFAAVLGEAVGESGRVLTVDTSRLASGAARAALVDLPQVEVVTGSVRRVLAVQPAGADLAVLDPPRSGAGREVVDLLAGAGVPRLIHIGCEAASFARDIGLYRGHGYAVEKIKVFDAFPLTHYVECVALLTRKV

1uwvA

0.21

0.20

0.92

3.22

MUSTER

--------QIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFG--VCGGCQQQHASVDLQQRSKSAALARLK--------HDVSEVI-ADVPWGYRRRARLSLNTQQLQGFRKAGSSDIVDVKQCPILAPQLEALLPKVRARHLGHVELVQATSGTLILRHHSEGLDLYLAPDILETVSGEPWYDSN--GLRLTFSPRDFIQVN-AGVNQKVARALEWLDVQPEDRVLDLFCG-GNFTLPLATQAASVVGVEGV-PALVEKGQQNARLNGLQNVTFYHENLEEDVPWAKNGFDKVLLDPARAGAAG--VQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARLA--LDFPHTGHLESVLFSR---

1uwvA

0.20

0.19

0.93

5.95

dPPAS

--------QIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFG--VCGGCQQQHASVDLQQRSKSAALARLK---------HDVSEVIADVPWGYRRRARLSLNTQQLQGFRKAGSSDIVDVKQCPILAPQLEALLPKVRARHLGHVELVQATSGTLILRHHSEGLDLYLAPDSEILETVSGEPWYDSNGLRLTFSPRDFIQVNAGVNQKVARALEWLD-VQPEDRVLDLFCG-GNFTLPLATQAASVVGVEG-VPALVEKGQQNARLNGLQNVTFYHENLEEDVPWAKNGFDKVLLDPARAGAAG--VQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARLA--LDFPHTGHLESVLFSR---

1uwvA

0.20

0.19

0.93

6.58

wdPPAS

--------QIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFG--VCGGCQQQHASVDLQQRSKSAALARLKHDVSE---------VIADVPWGYRRRARLSLNTQQLQGFRKAGSSDIVDVKQCPILAPQLEALLPKVRARHLGHVELVQATSGTLILRHHSEGLDLYLAPDSEILETVSGEPWYDSNGLRLTFSPRDFIQVNAGVNQKVARALEWL--VQPEDRVLDLFCG-GNFTLPLATQAASVVGVEGV-PALVEKGQQNARLNGLQNVTFYHENLEEDVPWAKNGFDKVLLDPARAGAA--GVQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARLA--LDFPHTGHLESVLFSR---

1uwvA

0.20

0.19

0.92

4.23

wMUSTER

--------QIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFG--VCGGCQQQHASVDLQQRSKSAALARLKHDVSE---------VIADVPWGYRRRARLSLNTQQLQGFRKAGSSDIVDVKQCPILAPQLEALLPKVRARHLGHVELVQATSG-TLILRHTAPLSSADREGLDLYLAPDSEPWYDSNGLRLTFSPRDFIQVNAGVNQKVARALEWL--VQPEDRVLDLFCG-GNFTLPLATQAASVVGVEGV-PALVEKGQQNARLNGLQNVTFYHENLEEDVPWAKNGFDKVLLDPARAGAAG--VQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARLA--LDFPHTGHLESVLFSR---

1uwvA

0.20

0.19

0.93

5.08

wPPAS

--------QIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFG--VCGGCQQQHASVDLQQRSKSAALARLKHDVSE---------VIADVPWGYRRRARLSLNTQQLQGFRKAGSSDIVDVKQCPILAPQLEALLPKVRARHLGHVELVQATSGTLILRHHSEGLDLYLAPDSEILETVSGEPWYDSNGLRLTFSPRDFIQVNAGVNQKVARALEWL--VQPEDRVLDLFCG-GNFTLPLATQAASVVGVEGV-PALVEKGQQNARLNGLQNVTFYHENLEEDVPWAKNGFDKVLLDPARAGAA--GVQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARL--ALDFPHTGHLESVLFSR---

1uwvA

0.21

0.19

0.92

10.50

dPPAS2

--------QIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFG--VCGGCQQQHASVDLQQRSKSAALARLK--------HDVSEVI-ADVPWGYRRRARLSLNTQQLQGFRKAGSSDIVDVKQCPILAPQLEALLPKVRARHLGHVELVQATSGTLILRHHSEGLDLAPDSEILETVSGEP--WYDSNGLRLTFSPRDFIQVNAGVNQKVARALEWLD-VQPEDRVLDLFCG-GNFTLPLATQAASVVGVEG-VPALVEKGQQNARLNGLQNVTFYHENLEEDVPWAKNGFDKVLLDPARAGAAG--VQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARLA--LDFPHTGHLESVLFSR---

1uwvA

0.21

0.19

0.92

4.77

PPAS

--------QIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFG--VCGGCQQQHASVDLQQRSKSAALARLKHDVSE---------VIADVPWGYRRRARLSLNTQQLQGFRKAGSSDIVDVKQCPILAPQLEALLPKVRARHLGHVELVQATSGTLILRHHSEGLDLAPDSEILETVSGEPWYDSN--GLRLTFSPRDFIQVNAGVNQKVARALEWLD-VQPEDRVLDLFCG-GNFTLPLATQAASVVGVEGV-PALVEKGQQNARLNGLQNVTFYHENLEEDVPWAKNGFDKVLLDPARAGAA--GVQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARLA--LDFPHTGHLESVLFSR---

1uwvA

0.21

0.19

0.92

6.83

Env-PPAS

--------QIITVSVNDLDSFGQGVARHNGKTLFIPGLLPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFG--VCGGCQQQHASVDLQQRSKSAALARLKHDVSE---------VIADVPWGYRRRARLSLNTQQLQGFRKAGSSDIVDVKQCPILAPQLEALLPKVRARHLGHVELVQATSGTLILRHHSEGLDLAPDSEILETVSGEPWYDSN--GLRLTFSPRDFIQVNAGVNQKVARALEWLD-VQPEDRVLDLFCG-GNFTLPLATQAASVVGVEGV-PALVEKGQQNARLNGLQNVTFYHENLEEDVPWAKNGFDKVLLDPARAGAA--GVQQIIKLEPIRIVYVSCNPATLARDSEALLKAGYTIARL--ALDFPHTGHLESVLFSR---

2vs1A

0.19

0.17

0.89

3.12

MUSTER

-------GSHMRGVIRKLNDDGFGVLK----GILVPFSAPGDEIIVERVERVKKRRVASQWKLVRSSPLRV----------KCGGCTLQHLNYDYQLEFKRKKLKRILG--------FEVEVVPSPKIFGHRNRIDLAITKD-GIGFR-EKWWKIVDIDEPVFGDEGFLRYMVLREGKFTEEVMVNFVTKEGDSIYWSVNRSKSDVSYGDIERFWGKEFIRERLDDVDYLIHPNSFFQTNSYQAVNLVRKVSELV---EGEKILDMYSGVGTFGIYLAKRGFNVKGFDSN-EFAIEMARRNVEINNVDAEFEVASDREVSV----KGFDTVIVDPPRAGLHPRLVKRLNREKPGVIVYVSCNPETFARDVKML---DYRIDEIVALDMFPHTPHVELVAKLV---

2vs1A

0.19

0.17

0.89

5.68

dPPAS

-------GSHMRGVIRKLNDDGFGVLK----GILVPFSAPGDEIIVERVERVKKRRVASQWKLVRSSPLRV----------KCGGCTLQHLNYDYQLEFKRKKLKRILG--------FEVEVVPSPKIFGHRNRIDLAITKD-GIGFR-EKWWKIVDIDEPVFGDEGFLRYMVLREGKFTEEVMVNFVTKEGDSIYWSVNRSKSDVSYGDIERFWGKEFIRERLDDVDYLIHPNSFFQTNSYQAVNLVRKVSELV---EGEKILDMYSGVGTFGIYLAKRGFNVKGFDS-NEFAIEMARRNVEINNVDAEFEVASDREVSV----KGFDTVIVDPPRAGLHPRLVKRLNREKPGVIVYVSCNPETFARDVKML---DYRIDEIVALDMFPHTPHVELVAKLV---

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 2 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.84

Estimated TM-score = 0.83±0.08

Estimated RMSD = 5.1±3.3Å

Download Model 2

C-score=0.05

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2vs1A	0.848	1.65	0.181	0.886
2	1uwvA	0.838	2.13	0.201	0.899
3	3bt7A	0.686	2.59	0.154	0.756
4	3vseA	0.553	5.20	0.061	0.738
5	4dmgA	0.546	5.16	0.117	0.721
6	2b78A	0.543	5.29	0.083	0.728
7	2as0A	0.537	5.27	0.102	0.728
8	3c0kA	0.536	5.44	0.080	0.741
9	2cwwB	0.532	5.37	0.122	0.724
10	3v8vA2	0.482	4.06	0.102	0.588

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.55	19	2vs1A	SAH	Rep, Mult	236,238,266,267,268,269,272,273,288,289,290,314,315,316,334,336
2	0.14	4	2bh2B	NUC	Rep, Mult	17,19,21,23,25,27,29,30,31,32,34,57,83,84,106,132,134,135,136,139,140,147,148,149,151,152,153,188,190,236,238,289,315,334,335,336,337,361,364,368,389,392,394,396
3	0.06	2	2bh2A	SF4	Rep, Mult	75,83,85,86,89,147,158,160,161,162,163
4	0.05	2	3bt7A	NUC	Rep, Mult	132,134,136,138,148,149,150,161,188,189,190,199,200,201,234,236,238,239,334,335,337,361,394,396
5	0.02	1	3cjqG	2MM	Rep, Mult	332,333,334,358,398

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.192	3bt7B	0.685	2.63	0.154	0.756	2.1.1.35	134,231,334
2	0.179	2bh2A	0.852	2.26	0.198	0.923	2.1.1.-	NA
3	0.060	1lehA	0.409	5.84	0.093	0.583	1.4.1.9	369
4	0.060	2x38A	0.409	6.67	0.042	0.664	2.7.1.153	360
5	0.060	2rd0A	0.354	7.71	0.035	0.642	2.7.1.153	251,377,383
6	0.060	3ixzA	0.418	6.73	0.047	0.674	3.6.3.10	NA
7	0.060	2bf4A	0.431	6.08	0.053	0.639	1.6.2.4	NA
8	0.060	2o7qA	0.434	5.71	0.063	0.617	4.2.1.10,1.1.1.25	NA
9	0.060	3i58A	0.425	5.09	0.092	0.558	2.1.1.-	NA
10	0.060	1tllA	0.426	6.41	0.075	0.649	1.14.13.39	NA
11	0.060	2as0A	0.537	5.27	0.102	0.728	2.1.1.-	NA
12	0.060	3b8eA	0.356	6.65	0.045	0.573	3.6.3.9	NA
13	0.060	1o0sA	0.438	6.34	0.073	0.649	1.1.1.38	NA
14	0.060	1aupA	0.398	5.99	0.066	0.568	1.4.1.2	NA
15	0.060	1kywC	0.424	5.06	0.070	0.556	2.1.1.68	NA
16	0.060	1f20A	0.352	6.04	0.032	0.514	1.14.13.39	340,359
17	0.060	1v9lA	0.411	5.97	0.060	0.595	1.4.1.2	NA
18	0.060	3b8eC	0.425	6.83	0.054	0.699	3.6.3.9	NA
19	0.060	1hrdA	0.422	6.52	0.069	0.644	1.4.1.2	100
20	0.060	1eulA	0.394	7.00	0.043	0.649	3.6.3.8	NA
21	0.060	3bt7A	0.686	2.59	0.154	0.756	2.1.1.35	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.41	0.850	1.65	0.18	0.89	2vs1A	GO:0003723 GO:0006396 GO:0008033 GO:0008168 GO:0008173 GO:0016740 GO:0030488 GO:0030697 GO:0032259 GO:0046872 GO:0051536 GO:0051539
1	0.35	0.852	2.26	0.20	0.92	2bh2A	GO:0003723 GO:0005506 GO:0006364 GO:0006396 GO:0008168 GO:0008173 GO:0016740 GO:0031167 GO:0032259 GO:0046872 GO:0051536 GO:0051539 GO:0070041 GO:0070475
2	0.16	0.532	5.37	0.12	0.72	2cwwB	GO:0005737 GO:0006364 GO:0008168 GO:0016740 GO:0032259
3	0.11	0.369	2.80	0.15	0.41	2fhpA	GO:0003676 GO:0008168 GO:0031167 GO:0032259
4	0.10	0.686	2.59	0.15	0.76	3bt7A	GO:0000049 GO:0005829 GO:0006396 GO:0008033 GO:0008168 GO:0008173 GO:0016740 GO:0019843 GO:0030488 GO:0030697 GO:0032259
5	0.07	0.494	4.29	0.07	0.61	3ay0B	GO:0005737 GO:0008033 GO:0008168 GO:0009019 GO:0016740 GO:0030488 GO:0032259 GO:0052906
6	0.07	0.390	3.65	0.12	0.46	3gdhA	GO:0000387 GO:0001510 GO:0005615 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006351 GO:0006355 GO:0008168 GO:0008173 GO:0009452 GO:0015030 GO:0016740 GO:0022613 GO:0030532 GO:0032259 GO:0036261 GO:0044255 GO:0071164 GO:0071167
7	0.07	0.371	2.48	0.16	0.41	3p9nA	GO:0003676 GO:0008168 GO:0031167 GO:0032259
8	0.07	0.546	5.16	0.12	0.72	4dmgA	GO:0003723 GO:0008168 GO:0016740 GO:0032259 GO:0046872 GO:0051536 GO:0051539
9	0.07	0.544	5.38	0.07	0.74	3c0kA	GO:0003723 GO:0005737 GO:0005829 GO:0006364 GO:0008168 GO:0008649 GO:0009383 GO:0016434 GO:0016740 GO:0031167 GO:0032259 GO:0044010 GO:0070475
10	0.07	0.546	5.23	0.10	0.74	2as0A	GO:0003723 GO:0008168 GO:0032259
11	0.06	0.427	3.28	0.07	0.50	3a27A	GO:0005737 GO:0006400 GO:0008033 GO:0016740 GO:0016765
12	0.06	0.414	4.09	0.08	0.50	2zwvA	GO:0000154 GO:0000179 GO:0003676 GO:0008168 GO:0008649 GO:0016740 GO:0031167 GO:0032259 GO:0046872 GO:0051536 GO:0051539
13	0.06	0.420	4.65	0.10	0.53	2r3sA
14	0.06	0.404	3.55	0.12	0.47	3cjtA	GO:0005737 GO:0006479 GO:0008168 GO:0008276 GO:0016740 GO:0032259
15	0.06	0.393	2.86	0.09	0.44	4qpnA	GO:0005622 GO:0005737 GO:0008168 GO:0016740 GO:0032259
16	0.06	0.385	2.76	0.09	0.43	1dusA	GO:0008168 GO:0016740 GO:0032259
17	0.06	0.406	2.84	0.09	0.45	3evzA	GO:0008168 GO:0032259
18	0.06	0.364	3.65	0.09	0.43	4necB	GO:0008168 GO:0016740 GO:0032259

Consensus prediction of GO terms

Molecular Function	GO:0051539	GO:0003723	GO:0030697	GO:0005506	GO:0070041
GO-Score	0.61	0.61	0.47	0.35	0.35
Biological Processes	GO:0030488	GO:0070475
GO-Score	0.47	0.35
Cellular Component	GO:0044424
GO-Score	0.33

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.