I-TASSER results

I-TASSER results for job id Rv2609c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (351 residues)
MTWLVLAGAVLLVVLVAFGAWGYQTANRLNRLNVRYDLSWQSLDSALARRAVVARAVAID
AYGGAPQGSRLAALADAAEGAPRHARENAENELSAALAMVNPASLPAALIAELADAEARV
LLARRFHNDAVRDTLALGERRLVRLLRLGGTAVLPTYFEIVERPHALVHGDQGASGRRTS
ARVVLLDDSGAVLLLCGSDPANPAFRDGAAPKWWFTVGGQVRPGERLAQAAARELAEETG
LRVAPADMIGPIWRRDEVFEFNGSLIDSEEFYLVHRTRRFEPAVQGRTELERRYIRDARW
CDANDIAQLVAAGERVYPLQLGELLPAANRLVDVALDNGAARDAGVPQPIR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTWLVLAGAVLLVVLVAFGAWGYQTANRLNRLNVRYDLSWQSLDSALARRAVVARAVAIDAYGGAPQGSRLAALADAAEGAPRHARENAENELSAALAMVNPASLPAALIAELADAEARVLLARRFHNDAVRDTLALGERRLVRLLRLGGTAVLPTYFEIVERPHALVHGDQGASGRRTSARVVLLDDSGAVLLLCGSDPANPAFRDGAAPKWWFTVGGQVRPGERLAQAAARELAEETGLRVAPADMIGPIWRRDEVFEFNGSLIDSEEFYLVHRTRRFEPAVQGRTELERRYIRDARWCDANDIAQLVAAGERVYPLQLGELLPAANRLVDVALDNGAARDAGVPQPIR
Prediction	CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEHCCCEEEEEEEEHCCCCCCCCEEEEEEEEEEEEEHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
Conf.Score	947999999999999999999999999889999999999999999999999999999998746756789999999999868989999999999999986576766589999999999998776555545578888875677545554577888876666667777777777777776665589998799978999874788776667778886557887679999999999999999988855555577667775545556776887789999999689876778887654665555444799999998765887775589999999999755567888888888887789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTWLVLAGAVLLVVLVAFGAWGYQTANRLNRLNVRYDLSWQSLDSALARRAVVARAVAIDAYGGAPQGSRLAALADAAEGAPRHARENAENELSAALAMVNPASLPAALIAELADAEARVLLARRFHNDAVRDTLALGERRLVRLLRLGGTAVLPTYFEIVERPHALVHGDQGASGRRTSARVVLLDDSGAVLLLCGSDPANPAFRDGAAPKWWFTVGGQVRPGERLAQAAARELAEETGLRVAPADMIGPIWRRDEVFEFNGSLIDSEEFYLVHRTRRFEPAVQGRTELERRYIRDARWCDANDIAQLVAAGERVYPLQLGELLPAANRLVDVALDNGAARDAGVPQPIR
Prediction	331213220222111222122101102102202310440141033004300400340044234436324302410440454547313401430141044144464345024204402420410230154335414524532202011012323212213233322222322333322222100000145111000113334334444443431000000202441313300200020005141534401010011212122332212120000000034230435423622452033114021620540373644021640140053034114122552333425425648
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEHCCCEEEEEEEEHCCCCCCCCEEEEEEEEEEEEEHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
MTWLVLAGAVLLVVLVAFGAWGYQTANRLNRLNVRYDLSWQSLDSALARRAVVARAVAIDAYGGAPQGSRLAALADAAEGAPRHARENAENELSAALAMVNPASLPAALIAELADAEARVLLARRFHNDAVRDTLALGERRLVRLLRLGGTAVLPTYFEIVERPHALVHGDQGASGRRTSARVVLLDDSGAVLLLCGSDPANPAFRDGAAPKWWFTVGGQVRPGERLAQAAARELAEETGLRVAPADMIGPIWRRDEVFEFNGSLIDSEEFYLVHRTRRFEPAVQGRTELERRYIRDARWCDANDIAQLVAAGERVYPLQLGELLPAANRLVDVALDNGAARDAGVPQPIR

2qkmB

0.16

0.11

0.70

1.12

wMUSTER

-------------------------------------------------------------------------------MSFTN--ATFSQVLDDLSARFILN-LPAEEQSSVERLCFQIEQAHWFYEDFIRAQNDQLPSLGLRVFSAKLFAHCPLLWKWSKVHEEAFDDFLRYKTRIPVRGAIMLDMSMQCVLVKGWK----------ASSGWGFPKGKIDKDESDVDCAIREVYEETGFDCSSRINPNEFIDMTIR--------GQNVRLYIIPGISLDTRFESRT---RKEISKIEWHNLMDLPTFKKNKPQVIPAPLKKWIKKRNIANNTTKEKNISVDVD-ADASS

2kdwA

0.19

0.08

0.44

2.08

wdPPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------MIDDDGYRPNVGIVICNRQGQVMWARRFG-----------QHSWQFPQGGINPGESAEQAMYRALFEEVGLSRKDVRILASTWLRYKLPDTKPVCIGQKQKWFLLQLVSGDAEINMQT-SSTPEFDGWRWVSYWYPVRQVSFKRDVYRRVMKEFASVVMSLQENTPK--------------

2qjoB

0.13

0.12

0.88

0.86

MUSTER

LRTLNLALEKAEQVIIILG--AADTRNPW-RSPERMAMIEACLSPQILKRVHFLT-VRDWLYSDNLWLAAVQQQVLKIGSNSVVVLGHRKDASSYYLNLFPQWDYETGHYPDFSSTAIRGAYFEGKEGDYLDKVPPAIADYLQTFQKSERYIALCDEYQ--FLQAYKQAWATAPYAPTFITTDAVVVQAGHVLMVRRQAK--------PGLGLIALPGGFIKQNETLVEGMLRELKEETRLKVPLPVLRGSIVDSHFDAPGRSLRGRTITHAYFIQLPGGELPAVKGGD----DAQKAWWMSLADLYAQEEQ----IYEDHFQIIQHFVSKV-------------------

1su2A

0.25

0.11

0.45

1.00

dPPAS

---------------------------------------------------------------------------------------------------------------------------------------------------------------------MEHDERTHVPVELRAAGVVLLNERGDILLVQEKG----IPGHPEKAGLWHIPSGAVEDGENPQDAAVREACEETGLRVRPVKFLGAYLGRFPDGV------LILRHVWLAEPEPGQTLAPAFTD----EIAEASFVSREDFAQLYAAG-QIRMYQTKLFYADALREKGFPALPV------------

4zb3A

0.10

0.07

0.66

1.23

wMUSTER

-------------------------------------------------------------------------------------------QI-PLL-TMVEPMDSEVFTESLRASLSHWREEGKLPLGLANLVEAAVS------EGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQE-----RSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKK------TDMFFLCVLS----PRSYDITEQKSEILQAKWMPIQEYVDQPWNKKN---EMFKFMANICQKKCEEEYLGFAIVPTTTSSG-K

2kdwA

0.19

0.08

0.44

2.35

wPPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------MIDDDGYRPNVGIVICNRQGQVMWARRFG-----------QHSWQFPQGGINPGESAEQAMYRALFEEVGLSRKDVRILASTWLRYKLPDTKPVCIGQKQKWFLLQLVSGDAEINMQTS-STPEFDGWRWVSYWYPVRQVSFKRDVYRRVMKEFASVVMSLQE------TPK---------

1su2A

0.25

0.11

0.45

1.81

dPPAS2

---------------------------------------------------------------------------------------------------------------------------------------------------------------------MEHDERTHVPVELRAAGVVLLNERGDILLVQEKG----IPGHPEKAGLWHIPSGAVEDGENPQDAAVREACEETGLRVRPVKFLGAYLGRFPDGV------LILRHVWLAEPEPGQTLAPAFTD----EIAEASFVSREDFAQLYAAGQI-------RMYQTKLFYADALREKGFPALPV------

2kdwA

0.19

0.08

0.44

1.53

PPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------MIDDDGYRPNVGIVICNRQGQVMWARRFG-----------QHSWQFPQGGINPGESAEQAMYRALFEEVGLSRKDVRILASTWLRYKLPDTKPVCIGQKQKWFLLQLVSGDAEINMQTS-STPEFDGWRWVSYWYPVRQVSFKRDVYRRVMKEFASVVMSLQENTPK--------------

4hfqA2

0.16

0.06

0.39

2.08

Env-PPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLMDVRAWIVE-DEKICLVRGQGED-----------SWALPGGFGEVGYSPTENILKEIEEETGFKAKVERLLAVFDT----NRFQLQSKQYTKFVFGCKLLDGQFQENQ-------EIADLQFFAIDQLPNL--SEKRITKEQIELLWQVYQGHRGQYLD--------------

4zb3A

0.10

0.07

0.67

0.84

MUSTER

------------------------------------------------------------------------------------------PLLEGET-TMVEPMDSEVFTESLRASLSHWREEGKLPLGLANLVEAAVS------EGFRYHHAEPEYLMLVSWISETPDTIPANASHVVGAGALVINKNTKEVLVVQE-----RSGFFKDKNVWKLPTGVINEGEDIWTGVAREVEEETGIIADFVEVLAFRQSHKAILKKK------TDMFFLCVLSP----RSYDITEQKSEILQAKWMPIQEYVDQPWNKKN---EMFKFMANICQKKCEEEYLGFAIVPTTTSSGKE

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.67

Estimated TM-score = 0.31±0.10

Estimated RMSD = 15.6±3.3Å

Download Model 2

C-score=-4.53

Download Model 3

C-score=-4.69

Download Model 4

C-score=-4.91

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2qjoB	0.666	4.50	0.108	0.860
2	2r5wB	0.661	4.47	0.089	0.852
3	1bxrA	0.443	6.64	0.063	0.749
4	2qkmB	0.437	4.59	0.102	0.570
5	1pj6A	0.411	6.41	0.063	0.675
6	3dupA	0.400	3.86	0.103	0.490
7	2va8B	0.399	6.32	0.066	0.638
8	1wygA	0.395	6.92	0.071	0.692
9	2zjaA	0.395	6.40	0.061	0.641
10	3dx4A	0.395	6.59	0.034	0.661

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.26	14	2a8pA	MN	Rep, Mult	218,219,234,238,294
2	0.10	6	3ffuA	MG	Rep, Mult	234,237,238
3	0.08	5	2qjoA	APR	Rep, Mult	178,180,182,197,218,219,220,234,238,268,316,320
4	0.03	2	1a9x1	III	Rep, Mult	113,115,116,138,139
5	0.02	1	1su2A	MG	Rep, Mult	180,218,219,270
6	0.02	1	2qjtB	MN	Rep, Mult	220,222,233,234
7	0.02	1	2ckjD	PO4	Rep, Mult	193,236,239,286

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.086	2r5wB	0.661	4.47	0.089	0.852	2.7.7.1	NA
2	0.085	2qjoB	0.666	4.50	0.108	0.860	2.7.7.1	86,93
3	0.060	2nz2A	0.373	6.17	0.057	0.593	6.3.4.5	NA
4	0.060	2ow6A	0.387	6.72	0.031	0.652	3.2.1.114	NA
5	0.060	2cqsA	0.342	6.86	0.033	0.578	2.4.1.20	225
6	0.060	1dl2A	0.378	6.52	0.060	0.624	3.2.1.113	28
7	0.060	1w6jA	0.383	6.27	0.068	0.621	5.4.99.7	215,218
8	0.060	2nq5A	0.373	7.33	0.047	0.675	2.1.1.14	NA
9	0.060	1nxcA	0.389	6.54	0.053	0.638	3.2.1.113	NA
10	0.060	1gqqA	0.313	6.60	0.066	0.524	6.3.2.8	NA
11	0.060	2vkzG	0.394	6.41	0.029	0.638	2.3.1.38,3.1.2.14	69,90,92
12	0.060	1f20A	0.270	6.94	0.034	0.464	1.14.13.39	35,110
13	0.060	2f00B	0.380	6.48	0.097	0.618	6.3.2.8	NA
14	0.060	2a6tA	0.368	3.97	0.132	0.453	3.6.1.30	234,237
15	0.060	3ecqB	0.375	7.02	0.068	0.652	3.2.1.97	NA
16	0.060	1gqyB	0.384	6.30	0.067	0.613	6.3.2.8	NA
17	0.060	3bcbA	0.378	6.01	0.054	0.581	2.9.1.2	115
18	0.060	1t3tA	0.377	6.69	0.071	0.632	6.3.5.3	138
19	0.060	3b9jC	0.335	6.96	0.041	0.578	1.17.3.2,1.17.1.4	NA
20	0.060	1t7lA	0.312	6.08	0.066	0.490	2.1.1.14	NA
21	0.060	1hxgA	0.383	6.51	0.053	0.650	4.2.3.9,4.1.99.7	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.37	0.661	4.47	0.09	0.85	2r5wB	GO:0000166 GO:0000309 GO:0003824 GO:0009058 GO:0016740 GO:0016779 GO:0016787 GO:0046872
1	0.18	0.351	3.23	0.13	0.41	3cngC	GO:0016787 GO:0046872
2	0.11	0.334	3.01	0.17	0.38	3gg6A	GO:0000287 GO:0005829 GO:0009117 GO:0016787 GO:0034656 GO:0044715 GO:0044716 GO:0044717 GO:0046057 GO:0046067 GO:0046712 GO:0046872
3	0.08	0.666	4.50	0.11	0.86	2qjoB	GO:0000166 GO:0000309 GO:0003824 GO:0005524 GO:0005737 GO:0008152 GO:0009058 GO:0009435 GO:0016740 GO:0016779 GO:0016787 GO:0019363
4	0.07	0.344	2.66	0.19	0.39	3dkuA	GO:0016787
5	0.06	0.376	4.22	0.07	0.48	4hfqA	GO:0016787
6	0.06	0.371	4.65	0.09	0.48	2gb5A	GO:0000210 GO:0000287 GO:0008270 GO:0016787 GO:0030145 GO:0035529 GO:0046872
7	0.06	0.360	3.18	0.14	0.42	3i9xA	GO:0016787
8	0.06	0.365	3.07	0.18	0.42	1su2A	GO:0000166 GO:0005524 GO:0016787
9	0.06	0.363	3.26	0.13	0.43	5bonA	GO:0005829 GO:0006203 GO:0008413 GO:0016787 GO:0017110 GO:0034656 GO:0035539 GO:0046872
10	0.06	0.365	3.39	0.09	0.43	2fmlA	GO:0016787
11	0.06	0.349	3.00	0.11	0.40	3fcmB	GO:0016787
12	0.06	0.341	2.94	0.14	0.39	3grnA	GO:0016787
13	0.06	0.338	3.66	0.16	0.41	2gt4A	GO:0000287 GO:0008727 GO:0009103 GO:0016787 GO:0030145 GO:0046872 GO:0047917
14	0.06	0.340	3.26	0.16	0.40	2pqvB	GO:0016787
15	0.06	0.333	3.19	0.14	0.39	2i8tB	GO:0000287 GO:0008727 GO:0016787 GO:0046872
16	0.06	0.343	2.83	0.18	0.39	2b0vA	GO:0016787
17	0.06	0.334	3.57	0.09	0.40	4zbpB	GO:0000210 GO:0005634 GO:0005737 GO:0005886 GO:0016020 GO:0016787 GO:0046872 GO:0047631
18	0.06	0.338	3.16	0.16	0.39	3fk9A	GO:0016787

Consensus prediction of GO terms

Molecular Function	GO:0016787	GO:0046872	GO:0000166	GO:0000309
GO-Score	0.61	0.55	0.42	0.42
Biological Processes	GO:0009058
GO-Score	0.42
Cellular Component	GO:0005829
GO-Score	0.11

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.