I-TASSER results

I-TASSER results for job id Rv2603c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (251 residues)
MSGHSKWATTKHKKAVVDARRGKMFARLIKNIEVAARVGGGDPAGNPTLYDAIQKAKKSS
VPNENIERARKRGAGEEAGGADWQTIMYEGYAPNGVAVLIECLTDNRNRAASEVRVAMTR
NGGTMADPGSVSYLFSRKGVVTLEKNGLTEDDVLAAVLEAGAEDVNDLGDSFEVISEPAE
LVAVRSALQDAGIDYESAEASFQPSVSVPVDLDGARKVFKLVDALEDSDDVQNVWTNVDV
SDEVLAALDDE

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MSGHSKWATTKHKKAVVDARRGKMFARLIKNIEVAARVGGGDPAGNPTLYDAIQKAKKSSVPNENIERARKRGAGEEAGGADWQTIMYEGYAPNGVAVLIECLTDNRNRAASEVRVAMTRNGGTMADPGSVSYLFSRKGVVTLEKNGLTEDDVLAAVLEAGAEDVNDLGDSFEVISEPAELVAVRSALQDAGIDYESAEASFQPSVSVPVDLDGARKVFKLVDALEDSDDVQNVWTNVDVSDEVLAALDDE
Prediction	CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEHCCCCEEEEEEEHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEHCEEEEEEHCCCCCCHHHHHHHHHHHCCCCEEHCCCEEEEEHCHHHHHHHHHHHHHHCCCCEEEEEEEHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHC
Conf.Score	99886477899999999999999999999999999999899987798999999999997999899999999975888898866899999985898899999866996789999999999879977899864444555899999599999999999999979964676599899998987899999999998998467899996688888999999999999999866866777534678999999999879
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MSGHSKWATTKHKKAVVDARRGKMFARLIKNIEVAARVGGGDPAGNPTLYDAIQKAKKSSVPNENIERARKRGAGEEAGGADWQTIMYEGYAPNGVAVLIECLTDNRNRAASEVRVAMTRNGGTMADPGSVSYLFSRKGVVTLEKNGLTEDDVLAAVLEAGAEDVNDLGDSFEVISEPAELVAVRSALQDAGIDYESAEASFQPSVSVPVDLDGARKVFKLVDALEDSDDVQNVWTNVDVSDEVLAALDDE
Prediction	76444324413442144145214202400420110034444416314301400440374613453044015404457554540430100021341000000010324530252024004326142056220321153201010346423034003201413052036475312010326305402510573715144020311052455146720530240053057273034012114044511541588
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEHCCCCEEEEEEEHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEHCEEEEEEHCCCCCCHHHHHHHHHHHCCCCEEHCCCEEEEEHCHHHHHHHHHHHHHHCCCCEEEEEEEHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHC
MSGHSKWATTKHKKAVVDARRGKMFARLIKNIEVAARVGGGDPAGNPTLYDAIQKAKKSSVPNENIERARKRGAGEEAGGADWQTIMYEGYAPNGVAVLIECLTDNRNRAASEVRVAMTRNGGTMADPGSVSYLFSRKGVVTLEKNGLTEDDVLAAVLEAGAEDVNDLGDSFEVISEPAELVAVRSALQDAGIDYESAEASFQPSVSVPVDLDGARKVFKLVDALEDSDDVQNVWTNVDVSDEVLAALDDE

1lfpA

0.46

0.44

0.96

3.66

MUSTER

----SHWAQIKHKKAKVDAQRGKLFSKLIREIIVATRLGGPNPEFNPRLRTAIEQAKKAN-PWENIERAIKKGAGEL-EGEQFEEVIYEGYAPGGVAVVLATT-DNRNRTTSEVRHVFTKHGGNLGASGCVSYLFERKGYIEVPAKEVSEEELLEKAIEVGAEDVQPGEEVHIIYTVPEELYEVKENLEKLGVPIEKAQITWKPISTVQIDEETAQKVIKLLNALEELDDVQQVIANFEIPEEILQK----

1lfpA

0.46

0.44

0.96

5.47

dPPAS

----SHWAQIKHKKAKVDAQRGKLFSKLIREIIVATRLGGPNPEFNPRLRTAIEQAKKAN-PWENIERAIKKGAGE-LEGEQFEEVIYEGYAPGGVAVVLATT-DNRNRTTSEVRHVFTKHGGNLGASGCVSYLFERKGYIEVPAKEVSEEELLEKAIEVGAEDVQPGEEVHIIYTVPEELYEVKENLEKLGVPIEKAQITWKPISTVQIDEETAQKVIKLLNALEELDDVQQVIANFEIPEEILQK----

1lfpA

0.45

0.43

0.96

5.77

wdPPAS

----SHWAQIKHKKAKVDAQRGKLFSKLIREIIVATRLGGPNPEFNPRLRTAIEQAKKAN-PWENIERAIKKGAGE-LEGEQFEEVIYEGYAPGG-VAVVLATTDNRNRTTSEVRHVFTKHGGNLGASGCVSYLFERKGYIEVPAKEVSEEELLEKAIEVGAEDVQPGEEVHIIYTVPEELYEVKENLEKLGVPIEKAQITWKPISTVQIDEETAQKVIKLLNALEELDDVQQVIANFEIPEEILQK----

1lfpA

0.46

0.44

0.95

4.28

wMUSTER

-----HWAQIKHKKAKVDAQRGKLFSKLIREIIVATRLGGPNPEFNPRLRTAIEQAKKAN-PWENIERAIKKGAGEL-EGEQFEEVIYEGYAPGGVAVVLATT-DNRNRTTSEVRHVFTKHGGNLGASGCVSYLFERKGYIEVPAKEVSEEELLEKAIEVGAEDVQPGEEVHIIYTVPEELYEVKENLEKLGVPIEKAQITWKPISTVQIDEETAQKVIKLLNALEELDDVQQVIANFEIPEEILQK----

1lfpA

0.46

0.44

0.96

5.17

wPPAS

----SHWAQIKHKKAKVDAQRGKLFSKLIREIIVATRLGGPNPEFNPRLRTAIEQAKKAN-PWENIERAIKKGAGE-LEGEQFEEVIYEGYAPGGVAVVLATT-DNRNRTTSEVRHVFTKHGGNLGASGCVSYLFERKGYIEVPAKEVSEEELLEKAIEVGAEDVQPGEEVHIIYTVPEELYEVKENLEKLGVPIEKAQITWKPISTVQIDEETAQKVIKLLNALEELDDVQQVIANFEIPEEILQK----

1lfpA

0.46

0.44

0.96

8.72

dPPAS2

----SHWAQIKHKKAKVDAQRGKLFSKLIREIIVATRLGGPNPEFNPRLRTAIEQAKKAN-PWENIERAIKKGAGE-LEGEQFEEVIYEGYAPGGVAVVLATT-DNRNRTTSEVRHVFTKHGGNLGASGCVSYLFERKGYIEVPAKEVSEEELLEKAIEVGAEDVQPGEEVHIIYTVPEELYEVKENLEKLGVPIEKAQITWKPISTVQIDEETAQKVIKLLNALEELDDVQQVIANFEIPEEILQK----

1lfpA

0.46

0.44

0.96

4.66

PPAS

----SHWAQIKHKKAKVDAQRGKLFSKLIREIIVATRLGGPNPEFNPRLRTAIEQAKKAN-PWENIERAIKKGAGE-LEGEQFEEVIYEGYAPGGVAVVLATT-DNRNRTTSEVRHVFTKHGGNLGASGCVSYLFERKGYIEVPAKEVSEEELLEKAIEVGAEDVQPGEEVHIIYTVPEELYEVKENLEKLGVPIEKAQITWKPISTVQIDEETAQKVIKLLNALEELDDVQQVIANFEIPEEILQK----

1lfpA

0.46

0.44

0.96

7.00

Env-PPAS

----SHWAQIKHKKAKVDAQRGKLFSKLIREIIVATRLGGPNPEFNPRLRTAIEQAKKAN-PWENIERAIKKGAGE-LEGEQFEEVIYEGYAPGGVAVVLATT-DNRNRTTSEVRHVFTKHGGNLGASGCVSYLFERKGYIEVPAKEVSEEELLEKAIEVGAEDVQPGEEVHIIYTVPEELYEVKENLEKLGVPIEKAQITWKPISTVQIDEETAQKVIKLLNALEELDDVQQVIANFEIPEEILQK----

1konA

0.48

0.45

0.92

3.53

MUSTER

--GHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLD-----ANPRLRAAVDKALSNNMTRDTLNRAIAR-------GANMETIIYEGYGPGGTAIMIECLSDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKG--DEDTIMEAALEAGAEDVVTYDDGIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMRLIDMLEDCDDVQEVYHNGEISDEVAATL---

1konA

0.48

0.45

0.92

5.35

dPPAS

--GHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLD-----ANPRLRAAVDKALSNNMTRDTLNRAIAR-------GANMETIIYEGYGPGGTAIMIECLSDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKG--DEDTIMEAALEAGAEDVVTYDDGIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMRLIDMLEDCDDVQEVYHNGEISDEVAATL---

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 1 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.56

Estimated TM-score = 0.93±0.06

Estimated RMSD = 2.7±2.0Å

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1lfpA	0.945	0.64	0.467	0.956
2	1konA	0.748	3.13	0.411	0.920
3	1mw7A	0.742	2.49	0.262	0.849
4	4f3qA	0.660	3.79	0.392	0.809
5	5ekzA	0.611	3.63	0.222	0.753
6	5amqA	0.430	5.53	0.044	0.673
7	3tijA	0.407	5.51	0.069	0.641
8	2wpnB	0.403	5.77	0.071	0.637
9	2frvJ	0.403	5.68	0.052	0.633
10	3uqyL	0.400	5.70	0.059	0.637

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.06	3	3b1rD	MG	Rep, Mult	33,70
2	0.04	2	1vf5A	CLA	Rep, Mult	32,36,56
3	0.04	2	3cgxA	IMD	Rep, Mult	6,25,105
4	0.04	2	4mb2D	ATP	Rep, Mult	20,27
5	0.02	1	1rxcA	R1P	Rep, Mult	4,68
6	0.02	1	3g1bA	MG	Rep, Mult	139,199
7	0.02	1	3quiC	ADP	Rep, Mult	91,95,124
8	0.02	1	2colA	POP	Rep, Mult	6,13,170
9	0.02	1	3rbxA	CA	Rep, Mult	129,180
10	0.02	1	4fevD	KAN	Rep, Mult	2,4
11	0.02	1	3ue8A	09M	Rep, Mult	41,91
12	0.02	1	4xk86	CLA	Rep, Mult	27,30,31
13	0.02	1	2b0qA	MG	Rep, Mult	229,230
14	0.02	1	1sijA	FES	Rep, Mult	34,35,36,39,41,42,71,73
15	0.02	1	1sijA	FES	Rep, Mult	37,38,39,41,45,46,47
16	0.02	1	2wpnB	SBY	Rep, Mult	36,39,41,42,49,52,53
17	0.02	1	5ijeA	ZN	Rep, Mult	18,21
18	0.02	1	2v0nA	GAV	Rep, Mult	80,81
19	0.02	1	4dkiB	BCT	Rep, Mult	223,226
20	0.02	1	2gagA	FMN	Rep, Mult	26,27,33,66,68

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2efyA	0.375	5.48	0.052	0.606	2.5.1.47	NA
2	0.060	1yniB	0.381	6.24	0.046	0.677	3.5.3.23	NA
3	0.060	3cmmC	0.395	6.01	0.045	0.689	6.3.2.19	22
4	0.060	2e0wB	0.379	5.53	0.049	0.598	2.3.2.2	NA
5	0.060	1v6cB	0.374	6.27	0.071	0.661	3.4.21.-	NA
6	0.060	1fohC	0.378	6.19	0.055	0.645	1.14.13.7	NA
7	0.060	1oqzB	0.380	6.06	0.052	0.641	3.5.1.93	NA
8	0.060	2z8kD	0.260	4.82	0.042	0.374	2.3.2.2	39,107
9	0.060	3dwbA	0.375	6.41	0.035	0.669	3.4.24.71	NA
10	0.060	1ddzA	0.381	5.37	0.060	0.594	4.2.1.1	NA
11	0.060	1vlbA	0.387	6.22	0.052	0.693	1.2.99.7	NA
12	0.060	1dgjA	0.379	6.38	0.067	0.701	1.2.-.-	199
13	0.060	1f2dA	0.391	6.02	0.052	0.673	3.5.99.7	10
14	0.060	3g5sA	0.378	5.70	0.038	0.614	2.1.1.74	221
15	0.060	1uiqD	0.384	5.52	0.038	0.625	4.2.3.1	NA
16	0.060	1pn0C	0.386	5.91	0.050	0.641	1.14.13.7	NA
17	0.060	1yq2A	0.386	6.15	0.040	0.669	3.2.1.23	67
18	0.060	1uwkA	0.386	5.79	0.045	0.641	4.2.1.49	NA
19	0.060	1ve1A	0.310	6.43	0.051	0.570	2.5.1.47	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.49	0.953	0.64	0.46	0.96	1lfpA	GO:0003677 GO:0005737 GO:0005829 GO:0006351 GO:0006355
1	0.42	0.676	3.81	0.39	0.83	4f3qA	GO:0003677 GO:0005737 GO:0005829 GO:0006351 GO:0006355
2	0.40	0.742	2.49	0.26	0.85	1mw7A	GO:0003677 GO:0005737 GO:0006351 GO:0006355
3	0.38	0.748	3.13	0.41	0.92	1konA	GO:0003677 GO:0005737 GO:0005829 GO:0006351 GO:0006355 GO:0010212
4	0.06	0.304	5.84	0.03	0.51	4qflA	GO:0043190 GO:0055085
5	0.06	0.354	6.08	0.03	0.60	2zj8A	GO:0000166 GO:0003676 GO:0003677 GO:0004386 GO:0005524 GO:0006281 GO:0006974 GO:0016787 GO:0016818
6	0.06	0.317	6.05	0.06	0.53	3p2cA	GO:0003824
7	0.06	0.325	6.08	0.05	0.55	3on6A	GO:0003824
8	0.06	0.284	6.33	0.07	0.50	2bklB	GO:0004252 GO:0006508 GO:0008236 GO:0070008
9	0.06	0.259	5.31	0.07	0.40	3u27C	GO:0005198 GO:0009605
10	0.06	0.291	6.14	0.05	0.49	3j4aA	GO:0019012 GO:0019028 GO:0019076 GO:0046718
11	0.06	0.298	6.24	0.03	0.52	4q6xA	GO:0006629 GO:0008081
12	0.06	0.305	5.55	0.07	0.49	4b5nA	GO:0000166 GO:0003824 GO:0003959 GO:0005829 GO:0010181 GO:0016491 GO:0042602 GO:0055114
13	0.06	0.261	6.40	0.04	0.47	5a1vK	GO:0000139 GO:0005179 GO:0005198 GO:0005576 GO:0005615 GO:0005737 GO:0005794 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0015031 GO:0016020 GO:0016192 GO:0030117 GO:0030126 GO:0030157 GO:0030663 GO:0031410 GO:0070062
14	0.06	0.319	6.22	0.06	0.56	3aquA	GO:0004553 GO:0004568 GO:0005576 GO:0005618 GO:0005975 GO:0006032 GO:0008061 GO:0008843 GO:0009651 GO:0009737 GO:0009753 GO:0035885
15	0.06	0.325	5.99	0.06	0.55	3d0cA	GO:0003824 GO:0008152 GO:0016829
16	0.06	0.276	5.68	0.06	0.46	3cjiA	GO:0000166 GO:0001172 GO:0003723 GO:0003724 GO:0003968 GO:0004197 GO:0004386 GO:0005198 GO:0005216 GO:0005524 GO:0006351 GO:0006508 GO:0006810 GO:0006811 GO:0008233 GO:0008234 GO:0016020 GO:0016032 GO:0016740 GO:0016779 GO:0016787 GO:0019012 GO:0019028 GO:0019030 GO:0019062 GO:0030430 GO:0033644 GO:0034220 GO:0039694 GO:0039707 GO:0044161 GO:0044162 GO:0044385 GO:0046718 GO:0051259
17	0.06	0.233	5.94	0.05	0.39	2fcaA	GO:0006400 GO:0008033 GO:0008168 GO:0008176 GO:0016740 GO:0032259 GO:0036265 GO:0043527
18	0.06	0.165	4.61	0.02	0.23	1wjoA	GO:0003779 GO:0005509 GO:0005737 GO:0032420 GO:0042491 GO:0046872 GO:0060348

Consensus prediction of GO terms

Molecular Function	GO:0003677
GO-Score	0.89
Biological Processes	GO:0006355	GO:0010212
GO-Score	0.89	0.38
Cellular Component	GO:0005829
GO-Score	0.82

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.