I-TASSER results

I-TASSER results for job id Rv2575

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (293 residues)
MTFNEGVQIDTSTTSTSGSGGGRRLAIGGGLGGLLVVVVAMLLGVDPGGVLSQQPLDTRD
HVAPGFDLSQCRTGADANRFVQCRVVATGNSVDAVWKPLLPGYTRPHMRLFSGQVGTGCG
PASSEVGPFYCPVDKTAYFDTDFFQVLVTQFGSSGGPFAEEYVVAHEYGHHVQNLLGVLG
RAQQGAQGAAGSGVRTELQADCYAGVWAYYASTVKQESTGVPYLEPLSDKDIQDALAAAA
AVGDDRIQQQTTGRTNPETWTHGSAAQRQKWFTVGYQTGDPNICDTFSAADLG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTFNEGVQIDTSTTSTSGSGGGRRLAIGGGLGGLLVVVVAMLLGVDPGGVLSQQPLDTRDHVAPGFDLSQCRTGADANRFVQCRVVATGNSVDAVWKPLLPGYTRPHMRLFSGQVGTGCGPASSEVGPFYCPVDKTAYFDTDFFQVLVTQFGSSGGPFAEEYVVAHEYGHHVQNLLGVLGRAQQGAQGAAGSGVRTELQADCYAGVWAYYASTVKQESTGVPYLEPLSDKDIQDALAAAAAVGDDRIQQQTTGRTNPETWTHGSAAQRQKWFTVGYQTGDPNICDTFSAADLG
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHCCCCCCCCCCCCCCCCCEHCCCCCEEEEHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCC
Conf.Score	97668875556644478888888877765689999999999988885555678888888888888777677777646667899999999999999999875568987999679999999888888886666899889986489999999988999778999999999999999988877888875555654678999999999999999998667776666555579989999999999998986556665898888888899999999999996555996658778886789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTFNEGVQIDTSTTSTSGSGGGRRLAIGGGLGGLLVVVVAMLLGVDPGGVLSQQPLDTRDHVAPGFDLSQCRTGADANRFVQCRVVATGNSVDAVWKPLLPGYTRPHMRLFSGQVGTGCGPASSEVGPFYCPVDKTAYFDTDFFQVLVTQFGSSGGPFAEEYVVAHEYGHHVQNLLGVLGRAQQGAQGAAGSGVRTELQADCYAGVWAYYASTVKQESTGVPYLEPLSDKDIQDALAAAAAVGDDRIQQQTTGRTNPETWTHGSAAQRQKWFTVGYQTGDPNICDTFSAADLG
Prediction	75247645446444543333333320321110000002112001100121244444445444444554653545552353032002102410352046315614413220124434121144453200000141210000131043026304556220010000000100000111212432453555353331330010000001001203535444443422420446204301400330213313543524344443311226202400320075341520111406638
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHCCCCCCCCCCCCCCCCCEHCCCCCEEEEHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCC
MTFNEGVQIDTSTTSTSGSGGGRRLAIGGGLGGLLVVVVAMLLGVDPGGVLSQQPLDTRDHVAPGFDLSQCRTGADANRFVQCRVVATGNSVDAVWKPLLPGYTRPHMRLFSGQVGTGCGPASSEVGPFYCPVDKTAYFDTDFFQVLVTQFGSSGGPFAEEYVVAHEYGHHVQNLLGVLGRAQQGAQGAAGSGVRTELQADCYAGVWAYYASTVKQESTGVPYLEPLSDKDIQDALAAAAAVGDDRIQQQTTGRTNPETWTHGSAAQRQKWFTVGYQTGDPNICDTFSAADLG

5cwdA

0.14

0.08

0.55

1.50

dPPAS2

------------------------------------------------------------------------TKEDARSTCEKAARKAAESNDEEVAKQAAKDCLEVAKQA--------GMPTKEA--------------ARSFCEAAARAAAESND-EEVAKIAAKACLEVAKQAGMPTKEAARSFCEAAARAAAESNDEEVAKIAAKACLEVAKQAGMPT--KEAARSFCEAAKRAAKESNDEEVEKIAKGMPWLE-----------------------------------

4ke2A

0.09

0.06

0.67

1.34

dPPAS2

--------------------------------------------------------MNIDPAARAAAAAAASKAAVTAADAAAAAATIAASAASVAAATAADDAAASIATINAASAAAKSIAAAAAMAAKDTAAAAASAAAAAVASAAKALETINVKAAYAAATTANTAAAAAAATATTAAAAAAAKATIDNAAAAKAAAVATAVSDAAATAATAAAVAAATLEAAAAKAAATAVSAAAAAAAAAIAFAAAP-----------------------------------------

4goxA

0.12

0.11

0.87

0.67

MUSTER

KQWQKPDHKNPNP-----------LSSPRSGSTLLRVMLAGHPGLYSPPELHLLPFETMGDRHQELGLSHLG------EGLQRALMDLENLTPEASQAKVNQWANTPIADIYAYLQRQAEQR------LLIDKSPSYGSDRHILDHSEILFDQ-----AKYIHLVR---HPYAVIESFTRLR------MDNPYALAESIWRTSNRNILDLGRTVGADRYLQVIYEDLVRDPRKVLTNICDFLGDEALLNRLTDVGDPNFLQHKDPALADKWRSI-TLPAALQLDTIQLAETFA

4bedB3

0.07

0.06

0.87

0.80

dPPAS

--------GLDHHEDHHSSSMAGHHEDIHEAEELRDALYKLQNDESHGGYEHIAGFHGYPNLCPEKGDEKYPCCVHGMSIFPHWHRLHTIQFERALKKHGSHLGIPYWDWTQTISSLPDSGNNNPFFKYHIRSINQDTVSSIFYLALQALEEDNYCDFEVQYEILHNEVHALIGGAEKYSMSTLEYSAFDPYFMIHHASLDKIWIIWQELQKRRVKPAHAGSCAGFNYESVNNDDFTRENSLPNAVVDSHRFNYKYDNLNLHGH-----------------------------

3c37A

0.19

0.12

0.62

0.77

wdPPAS

------------------------------------------------------------KGFNISIEQEKELGNKFAVEIEK--PEVQRYVDKVGKRLLSGARAVVFKVVK----------DDSVNAFAIP-GGRVYVHTGLLKAADN-------ETELAGVLAHEINHAVARHG-LAQLAGQLFGKAGSYSREYENQADFLGVETYKA-NGLTSFFQKLNATHPLTSERIQRVQAEIAKL--------PPQRYLTDETEFKKIKGRLKLE---------------------

3be7A2

0.15

0.13

0.88

0.65

wMUSTER

SKGEESIADSSHMGTVWGVVNAEKTNVGAANYADVSVRDAIERGVINGPMLVSGPALGITGGHCDHNLLPPEFNYSSEGVVDS-----PWEARKMVRKNRKYGAD-LIKFCATG-----GVMSRNTDV------NAKQFTLEEMKAIVDEAHNHG-----MKVAAHAHG-----LIGIKAAIKAG-SFIDDETIDMAIKNNTVLFVSDYILGEGAKAGIREESLNKERGKKQRENFMNAHRRGAIIT-----GTDAG-IFDHGDAKQFAYMVEWGMTPLEAIQASTIKTATFG

3c37A

0.18

0.11

0.58

0.75

wPPAS

------------------------------------------------------------KGFNISIEQEKELGNKFAVEIEK--PEVQRYVDKVGKRLLSGARAVVFKVVK----------DDSVNAFAIP-GGRVYVHTGLLKAADN-------ETELAGVLAHEINHAVAR---NLAQLAGQLFGKA--SREYENQADFLGVETYKA-------------YNP----------NGLTSF--QKLNA-----------THPLTSERIQRVQAEIAKLPP---QRYLTDETE

4bedB3

0.10

0.09

0.83

1.30

dPPAS2

--------GLDHHEDHHSSSMAGHHEDHHEAEELRDALYKLQNDESHGGYEHIGYPNLCPEKGDEKYPCCVHGMSIFPHWHRLHTIQFERALKKHWDWTQTISSLPTFFADSGNNNPFFKYHIRSINQDTVQQTKFGEFSSIFYLALQALEEDNYCDFEVQYEILHNEVHALIGGAEKYSMSTLEYSAFDPYFMIHHASLDKIWIIWQELQKRRVKPAHAGSNYESVNNDDFTRENSLPNAVVDDNL--NLHGH---------------------------------------

5gaoE

0.10

0.98

0.89

PPAS

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-----AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK-

3c37A

0.15

0.10

0.67

0.84

Env-PPAS

------------------------------------------------------------KGFNISIEQEKELGNKFAVEIEKQQPEVQRYVDKVGKRLLSGARAVVFKVVK----------DDSVNAFAIP-GGRVYVHTGLLKAADN-------ETELAGVLAHEINHAVAR------HGTRQTQEYGYSLVLSLVLGDNLAQLAGQLFG------KAGSYSREYENQADFLGVETYKAGYNPNGLTSFFQKL---NATHPLTSERIQRVQAEIAKLPP---QRYLTDETE

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.76

Estimated TM-score = 0.23±0.06

Estimated RMSD = 18.3±2.3Å

Download Model 2

C-score=-5.00

Download Model 3

C-score=-5.00

Download Model 4

C-score=-4.45

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4k90A	0.482	5.72	0.101	0.747
2	4ka7A2	0.450	5.72	0.078	0.707
3	1pn0C	0.435	5.64	0.047	0.683
4	3ihgA	0.433	5.66	0.069	0.679
5	3nqxA	0.432	5.70	0.070	0.676
6	2cse1	0.432	6.72	0.048	0.754
7	1y7912	0.431	5.58	0.066	0.669
8	2qa1A	0.431	5.68	0.053	0.669
9	3dbkA	0.429	5.86	0.089	0.676
10	5fp1A	0.429	6.56	0.032	0.744

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.20	11	3dtiA	ZN	Rep, Mult	166,167,170,197
2	0.09	5	4fgmA	ZN	Rep, Mult	162,166,197
3	0.06	3	3r2lA	FE	Rep, Mult	137,167,170
4	0.04	2	2e84A	ZN	Rep, Mult	197,201
5	0.04	2	1fjqA	ACN	Rep, Mult	92,110,168,172
6	0.04	2	3c37A	ZN	Rep, Mult	166,170,197,262
7	0.02	1	1n38A	CH1	Rep, Mult	119,120,130,131,133,134
8	0.02	1	2a68P	MG	Rep, Mult	201,204

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1fohC	0.426	5.82	0.043	0.679	1.14.13.7	NA
2	0.060	3ebgA	0.395	5.80	0.050	0.614	3.4.11.-	167
3	0.060	1gz3A	0.370	6.42	0.038	0.642	1.1.1.38	NA
4	0.060	8tlnE	0.423	5.86	0.095	0.662	3.4.24.27	NA
5	0.060	1fj3A	0.422	5.87	0.095	0.662	3.4.24.27	167
6	0.060	1ezmA	0.427	5.63	0.066	0.662	3.4.24.26	167
7	0.060	1jxaA	0.398	6.32	0.061	0.659	2.6.1.16	NA
8	0.060	1w18B	0.402	5.81	0.033	0.642	2.4.1.10	NA
9	0.060	2e1pA	0.336	6.82	0.053	0.618	3.4.21.62	NA
10	0.060	1thgA	0.412	6.39	0.052	0.703	3.1.1.3	NA
11	0.060	2b0tA	0.404	5.68	0.056	0.645	1.1.1.42	175,243
12	0.060	1mosA	0.373	6.23	0.059	0.608	2.6.1.16	164
13	0.060	1ej6A	0.399	6.45	0.057	0.676	2.7.7.50	NA
14	0.060	2vf4X	0.371	6.11	0.055	0.614	2.6.1.16	NA
15	0.060	1bxrA	0.416	6.04	0.044	0.672	6.3.5.5	109
16	0.060	1espA	0.421	5.85	0.076	0.659	3.4.24.28	NA
17	0.060	2o36A	0.425	5.50	0.067	0.648	3.4.24.15	207
18	0.060	1i1iP	0.424	5.55	0.062	0.659	3.4.24.16	162
19	0.060	1ii2A	0.395	6.18	0.062	0.642	4.1.1.49	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.18	0.424	5.55	0.06	0.66	1i1iP	GO:0004222 GO:0005737 GO:0005739 GO:0005758 GO:0005829 GO:0006508 GO:0006518 GO:0008233 GO:0008237 GO:0016787 GO:0046872 GO:0070012
1	0.07	0.433	5.66	0.07	0.68	3ihgA	GO:0000166 GO:0016491 GO:0016709 GO:0017000 GO:0055114 GO:0071949 GO:1901771
2	0.07	0.482	5.72	0.10	0.75	4k90A	GO:0004222 GO:0005576 GO:0005615 GO:0006508 GO:0008233 GO:0008237 GO:0008270 GO:0009405 GO:0016787 GO:0046872
3	0.07	0.392	6.13	0.03	0.65	2dkiA	GO:0016491 GO:0018668 GO:0042803 GO:0055114 GO:0071949
4	0.07	0.426	5.82	0.04	0.68	1fohC	GO:0004497 GO:0005737 GO:0016491 GO:0018662 GO:0019336 GO:0019439 GO:0055114 GO:0071949
5	0.07	0.420	5.90	0.07	0.67	4k5rB	GO:0000166 GO:0016491 GO:0055114 GO:0071949
6	0.07	0.429	5.42	0.07	0.66	1y791	GO:0004180 GO:0004222 GO:0005737 GO:0005829 GO:0006508 GO:0006518 GO:0008233 GO:0008237 GO:0016787 GO:0030288 GO:0046872
7	0.06	0.421	5.85	0.08	0.66	1espA	GO:0004222 GO:0005576 GO:0006508 GO:0008233 GO:0008237 GO:0016787 GO:0046872
8	0.06	0.427	5.62	0.05	0.66	2qa1A	GO:0000166 GO:0016491 GO:0055114 GO:0071949
9	0.06	0.426	5.71	0.07	0.68	2r0gA	GO:0000166 GO:0004497 GO:0008168 GO:0016491 GO:0032259 GO:0055114 GO:0071949
10	0.06	0.425	5.50	0.07	0.65	2o36A	GO:0004222 GO:0005737 GO:0005758 GO:0006508 GO:0006518 GO:0008233 GO:0008237 GO:0016787 GO:0042277 GO:0046872
11	0.06	0.420	5.76	0.04	0.66	4x4jA	GO:0016491 GO:0055114 GO:0071949
12	0.06	0.417	5.81	0.07	0.67	4k2xB	GO:0000166 GO:0016491 GO:0055114 GO:0071949
13	0.06	0.422	5.83	0.06	0.68	4cy8A	GO:0004497 GO:0016491 GO:0055114 GO:0071949
14	0.06	0.420	5.81	0.05	0.68	4icyA	GO:0000166 GO:0016491 GO:0055114 GO:0071949
15	0.06	0.423	5.82	0.06	0.68	4z2rA	GO:0004497 GO:0016491 GO:0055114 GO:0071949
16	0.06	0.353	5.69	0.04	0.55	2qa2A	GO:0000166 GO:0016491 GO:0055114 GO:0071949
17	0.06	0.375	6.55	0.03	0.66	3efmA	GO:0004872 GO:0005506 GO:0006810 GO:0009279 GO:0015891 GO:0016020
18	0.06	0.377	5.46	0.05	0.57	3nixA	GO:0000166 GO:0016491 GO:0055114 GO:0071949

Consensus prediction of GO terms

Molecular Function	GO:0004175	GO:0008237	GO:0043169	GO:0050660
GO-Score	0.47	0.47	0.47	0.37
Biological Processes	GO:0019538	GO:0044710	GO:1901564	GO:0043603
GO-Score	0.47	0.37	0.37	0.37
Cellular Component	GO:0031970	GO:0005740
GO-Score	0.37	0.37

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.