I-TASSER results

I-TASSER results for job id Rv2573

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (246 residues)
MVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQ
PHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPD
FTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVV
DEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAA
ASDGPG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG
Prediction	CCCCCCCCCHHHCCCCCCEEEEHCCCCCHHHHHHHHHHHCCCCCEEEEEHCCHHHHHHHHHHCCCCCCCCEEEEHCCCCCCCCEEEEHCCCEEHCCCCCHHHHHHHHHCCCCCEEEHCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
Conf.Score	988565789888788876899965898769999999998799968999866856899998768998866599986565589968998578768779995799999999589986886797789999999999999999877555546674799999999999999999998888996799999999998999876778999998998755656779998779989998568899998887587999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG
Prediction	714030303165043201000000102127401400441036701000000224034203421564300000000101333411021125030301626404300400662704042354022100320010011101001423121143640240032004000200312316156620430142036225522100110234433101000000001205626130200300220010247468
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCHHHCCCCCCEEEEHCCCCCHHHHHHHHHHHCCCCCEEEEEHCCHHHHHHHHHHCCCCCCCCEEEEHCCCCCCCCEEEEHCCCEEHCCCCCHHHHHHHHHCCCCCEEEHCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
MVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

4ol9A

1.00

2.28

MUSTER

VVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

4ol9A

1.00

3.63

dPPAS

VVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

4ol9A

1.00

3.70

wdPPAS

VVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

4ol9A

1.00

2.50

wMUSTER

VVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

4ol9A

1.00

2.68

wPPAS

VVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

4ol9A

1.00

5.93

dPPAS2

VVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

4ol9A

1.00

2.54

PPAS

VVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

4ol9A

1.00

3.74

Env-PPAS

VVPGPVHTSPREVAGPVDVLILAVKATQNDAARPWLTRLCDERTVVAVLQNGVEQVEQVQPHCPSSAVVPAIVWCSAETQPQGWVRLRGEAALVVPTGPAAEQFAGLLRGAGATVDCDPDFTTAAWRKLLVNALAGFMVLSGRRSAMFRRDDVAALSRRYVAECLAVARAEGARLDDDVVDEVVRLVRSAPQDMGTSMLADRAAHRPLEWDLRNGVIVRKARAHGLATPISDVLVPLLAAASDGPG

3g17G

0.31

0.30

0.98

1.90

MUSTER

PAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTYLAHEDTLIILAQNGYGQLE----HIPFKNVCQAVVYISGQKKGDVVTHFRD-YQLRIQDNALTRQFRDLVQDSQIDIVLEANIQQAIWYKLLVNLGINSITALGRTVAIMHNPEIRILCRQLLLDGCRVAQAEGLNFSEQTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIYSFLRAYQQNEG

3g17G

0.31

0.30

0.98

2.92

dPPAS

PAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTYLAHEDTLIILAQNGYGQLE----HIPFKNVCQAVVYISGQKKG-DVVTHFRDYQLRIQDNALTRQFRDLVQDSQIDIVLEANIQQAIWYKLLVNLGINSITALGRTVAIMHNPEIRILCRQLLLDGCRVAQAEGLNFSEQTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIYSFLRAYQQNEG

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 1 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.45

Estimated TM-score = 0.92±0.06

Estimated RMSD = 2.9±2.1Å

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4ol9A	0.969	1.18	0.984	1.000
2	3g17G	0.889	1.99	0.286	0.980
3	3hn2A	0.885	2.23	0.239	0.988
4	3ghyA	0.856	2.47	0.229	0.976
5	3i83A	0.851	2.78	0.216	0.996
6	3hwrA	0.849	2.53	0.279	0.976
7	2ofpB	0.847	2.63	0.207	0.980
8	5ayvA	0.832	2.81	0.219	0.984
9	3egoA	0.791	3.05	0.182	0.959
10	3wfiA	0.754	3.83	0.243	0.988

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.43	17	3wfjG	NAD	Rep, Mult	23,24,25,28,50,51,73,75,76,77,79,128,206,209
2	0.18	7	5ayvB	KPL	Rep, Mult	51,74,128,132,135,136,145,194,196,197
3	0.15	6	5x20B	AOT	Rep, Mult	51,74,76,128,132,196,197
4	0.02	1	3ego0	III	Rep, Mult	140,141,142,152,153,156,159,230,232,233,234,236,237,240

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.500	3ghyA	0.856	2.47	0.229	0.976	1.1.1.169	128
2	0.454	3i83A	0.851	2.78	0.216	0.996	1.1.1.169	128
3	0.099	3g17G	0.889	1.99	0.286	0.980	1.1.1.169	128
4	0.069	3egoA	0.791	3.05	0.182	0.959	1.1.1.169	128
5	0.069	3hn2A	0.885	2.23	0.239	0.988	1.1.1.169	128
6	0.067	1txgB	0.679	4.23	0.109	0.927	1.1.1.94	132,195
7	0.067	2plaA	0.674	4.25	0.127	0.923	1.1.1.8	136
8	0.066	1x0xA	0.669	4.24	0.135	0.923	1.1.1.8	136
9	0.066	2i9pB	0.569	4.37	0.074	0.797	1.1.1.31	117,122
10	0.065	2ofpB	0.847	2.63	0.207	0.980	1.1.1.169	128
11	0.060	1yrlA	0.588	4.78	0.103	0.866	1.1.1.86	NA
12	0.060	3c7aA	0.728	3.92	0.115	0.959	1.5.1.11	NA
13	0.060	3k96A	0.681	4.30	0.130	0.935	1.1.1.94	136
14	0.060	3dojA	0.571	4.55	0.083	0.817	1.1.1.79	NA
15	0.060	1qmgA	0.567	4.95	0.073	0.854	1.1.1.86	182
16	0.060	2qytA	0.718	2.81	0.186	0.854	1.1.1.169	21,49,52,128
17	0.060	3c7cB	0.741	3.83	0.115	0.968	1.5.1.11	24
18	0.060	2ew2B	0.664	4.75	0.179	0.943	1.1.1.169	NA
19	0.060	2h78A	0.558	4.67	0.130	0.805	1.1.1.31	124
20	0.060	1bg6A	0.754	3.69	0.107	0.976	1.5.1.28	NA
21	0.060	1n1eA	0.674	4.36	0.121	0.939	1.1.1.8	NA
22	0.060	3i83B	0.814	2.61	0.222	0.935	1.1.1.169	128
23	0.060	3ckyC	0.552	4.55	0.093	0.785	1.1.1.291	73
24	0.060	1txgA	0.668	4.22	0.105	0.915	1.1.1.94	NA
25	0.060	1yj8A	0.652	4.47	0.076	0.919	1.1.1.8	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.71	0.969	1.18	0.98	1.00	4ol9A	GO:0005737 GO:0005829 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
1	0.37	0.889	1.99	0.29	0.98	3g17G	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
2	0.37	0.808	2.77	0.23	0.94	3wfjF	GO:0000166 GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
3	0.37	0.847	2.63	0.21	0.98	2ofpB	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0033317 GO:0050661 GO:0055114
4	0.36	0.851	2.78	0.22	1.00	3i83A	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
5	0.35	0.856	2.47	0.23	0.98	3ghyA	GO:0008677 GO:0016491 GO:0050661 GO:0055114
6	0.35	0.678	4.72	0.18	0.97	2ew2B	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
7	0.35	0.842	2.78	0.22	0.98	5ayvB	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
8	0.33	0.885	2.23	0.24	0.99	3hn2A	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
9	0.30	0.865	2.48	0.28	0.99	3hwrA	GO:0000166 GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
10	0.28	0.791	3.05	0.18	0.96	3egoA	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
11	0.28	0.749	2.79	0.17	0.89	2qytA	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
12	0.17	0.814	2.61	0.22	0.94	3i83B	GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
13	0.11	0.695	4.46	0.20	0.97	3wfiB	GO:0000166 GO:0005737 GO:0008677 GO:0015940 GO:0016491 GO:0050661 GO:0055114
14	0.10	0.681	4.30	0.13	0.94	3k96A	GO:0004367 GO:0005737 GO:0005975 GO:0006072 GO:0006629 GO:0006650 GO:0008654 GO:0009331 GO:0016491 GO:0016616 GO:0036439 GO:0046167 GO:0046168 GO:0047952 GO:0051287 GO:0055114
15	0.07	0.585	4.71	0.10	0.85	3ulkA	GO:0000287 GO:0004455 GO:0005829 GO:0008652 GO:0009082 GO:0009097 GO:0009099 GO:0016491 GO:0050661 GO:0055114
16	0.06	0.350	6.43	0.04	0.63	2dwcB	GO:0000166 GO:0000287 GO:0003824 GO:0004644 GO:0005524 GO:0006164 GO:0006189 GO:0009152 GO:0016740 GO:0016742 GO:0043815 GO:0046872
17	0.06	0.343	5.71	0.02	0.55	4x9mA	GO:0016491 GO:0055114
18	0.06	0.298	6.45	0.09	0.54	5hmsA	GO:0001666 GO:0003824 GO:0004655 GO:0005615 GO:0005634 GO:0005829 GO:0006779 GO:0006782 GO:0006783 GO:0006979 GO:0007584 GO:0008152 GO:0008270 GO:0009635 GO:0009636 GO:0009725 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010043 GO:0010044 GO:0010212 GO:0010266 GO:0010269 GO:0010288 GO:0014070 GO:0014823 GO:0016829 GO:0031667 GO:0032025 GO:0032496 GO:0032791 GO:0033014 GO:0033197 GO:0033273 GO:0042493 GO:0042802 GO:0043200 GO:0045471 GO:0046685 GO:0046686 GO:0046689 GO:0046872 GO:0051260 GO:0051384 GO:0051597 GO:0070062 GO:0070541 GO:0070542 GO:0071284 GO:0071353

Consensus prediction of GO terms

Molecular Function	GO:0008677	GO:0050661
GO-Score	0.95	0.95
Biological Processes	GO:0055114	GO:0033317
GO-Score	0.95	0.37
Cellular Component	GO:0005829
GO-Score	0.71

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.