I-TASSER results

I-TASSER results for job id Rv2559c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (452 residues)
MPEAVSDGLFDVPGVPMTSGHDLGASAGAPLAVRMRPASLDEVVGQDHLLAPGSPLRRLV
EGSGVASVILYGPPGSGKTTLAALISQATGRRFEALSALSAGVKEVRAVIENSRKALLHG
EQTVLFIDEVHRFSKTQQDALLSAVEHRVVLLVAATTENPSFSVVAPLLSRSLILQLRPL
TAEDTRAVVQRAIDDPRGLGRAVAVAPEAVDLLVQLAAGDARRALTALEVAAEAAQAAGE
LVSVQTIERSVDKAAVRYDRDGDQHYDVVSAFIKSVRGSDVDAALHYLARMLVAGEDPRF
IARRLMILASEDIGMAGPSALQVAVAAAQTVALIGMPEAQLTLAHATIHLATAPKSNAVT
TALAAAMNDIKAGKAGLVPAHLRDGHYSGAAALGNAQGYKYSHDDPDGVVAQQYPPDELV
DVDYYRPTGRGGEREIAGRLDRLRAIIRKKRG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MPEAVSDGLFDVPGVPMTSGHDLGASAGAPLAVRMRPASLDEVVGQDHLLAPGSPLRRLVEGSGVASVILYGPPGSGKTTLAALISQATGRRFEALSALSAGVKEVRAVIENSRKALLHGEQTVLFIDEVHRFSKTQQDALLSAVEHRVVLLVAATTENPSFSVVAPLLSRSLILQLRPLTAEDTRAVVQRAIDDPRGLGRAVAVAPEAVDLLVQLAAGDARRALTALEVAAEAAQAAGELVSVQTIERSVDKAAVRYDRDGDQHYDVVSAFIKSVRGSDVDAALHYLARMLVAGEDPRFIARRLMILASEDIGMAGPSALQVAVAAAQTVALIGMPEAQLTLAHATIHLATAPKSNAVTTALAAAMNDIKAGKAGLVPAHLRDGHYSGAAALGNAQGYKYSHDDPDGVVAQQYPPDELVDVDYYRPTGRGGEREIAGRLDRLRAIIRKKRG
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEHCCCCCCHHHHHHHHHHHHCCCEEEEHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHCHHHCCHHHHHHHHHHHHCCEEEEEEHCCCCCCCCCCHHHHHHHEEEEEHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
Conf.Score	98755555456788887666665666789868986999888865755445898489999998999779997899988999999999998995899754568889999999999999988997799996766689899999999876586999997588987544567887644678665999999999999998677658899899999999999969899999999999999868898566699999998776665489986458999999999876999999999999999899999999999999999875589899999999999999989955778999999999859986899999999999999889999986657788667898799888789988899876567799666987556789986899999999999999998669
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MPEAVSDGLFDVPGVPMTSGHDLGASAGAPLAVRMRPASLDEVVGQDHLLAPGSPLRRLVEGSGVASVILYGPPGSGKTTLAALISQATGRRFEALSALSAGVKEVRAVIENSRKALLHGEQTVLFIDEVHRFSKTQQDALLSAVEHRVVLLVAATTENPSFSVVAPLLSRSLILQLRPLTAEDTRAVVQRAIDDPRGLGRAVAVAPEAVDLLVQLAAGDARRALTALEVAAEAAQAAGELVSVQTIERSVDKAAVRYDRDGDQHYDVVSAFIKSVRGSDVDAALHYLARMLVAGEDPRFIARRLMILASEDIGMAGPSALQVAVAAAQTVALIGMPEAQLTLAHATIHLATAPKSNAVTTALAAAMNDIKAGKAGLVPAHLRDGHYSGAAALGNAQGYKYSHDDPDGVVAQQYPPDELVDVDYYRPTGRGGEREIAGRLDRLRAIIRKKRG
Prediction	64663354015355453455453645622000210105304110105100367420330065530000000012100000001000530624022000133316303510550363344551000000000101450041004204512000000001112140230010001103045044710250043005315423651502440031006105200010000000002002345330225103500452123102512411310120030044530310030013006304301200110021013101201240041032002003202102021000000010010110110040043015005736526003101102151055133153030014266211515100670564500413734204402510530261257668
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEHCCCCCCHHHHHHHHHHHHCCCEEEEHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHCHHHCCHHHHHHHHHHHHCCEEEEEEHCCCCCCCCCCHHHHHHHEEEEEHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
MPEAVSDGLFDVPGVPMTSGHDLGASAGAPLAVRMRPASLDEVVGQDHLLAPGSPLRRLVEGSGVASVILYGPPGSGKTTLAALISQATGRRFEALSALSAGVKEVRAVIENSRKALLHGEQTVLFIDEVHRFSKTQQDALLSAVEHRVVLLVAATTENPSFSVVAPLLSRSLILQLRPLTAEDTRAVVQRAIDDPRGLGRAVAVAPEAVDLLVQLAAGDARRALTALEVAAEAAQAAGELVSVQTIERSVDKAAVRYDRDGDQHYDVVSAFIKSVRGSDVDAALHYLARMLVAGEDPRFIARRLMILASEDIGMAGPSALQVAVAAAQTVALIGMPEAQLTLAHATIHLATAPKSNAVTTALAAAMNDIKAGKAGLVPAHLRDGHYSGAAALGNAQGYKYSHDDPDGVVAQQYPPDELVDVDYYRPTGRGGEREIAGRLDRLRAIIRKKRG

3pvsA

0.42

0.38

0.90

3.45

MUSTER

----------------------------QPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRF------AFLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGQIVLPDETRRAIAELVNGDARRALNTLEMMADMAESGKRVLKPELLTEIAGER---SARKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPT----------EYRYAHDEANAYAAEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPI

3pvsA

0.42

0.38

0.90

5.22

dPPAS

----------------------------QPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFA------FLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGQIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSVLKPELLTEIAGER---SARKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPT----------EYRYAHDEANAYAAEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPI

3pvsA

0.43

0.39

0.90

5.91

wdPPAS

----------------------------QPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFA------FLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSGKRVLKPELLTEIAGERSARKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPT----------EYRYAHDEANAYAAEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPI

3pvsA

0.42

0.38

0.89

4.03

wMUSTER

-----------------------------PLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFA------FLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGQIVLPDETRRAIAELVNGDARRALNTLEMMADMAESGKRVLKPELLTEIAGER---SARKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPT----------EYRYAHDEANAYAAEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPI

3pvsA

0.42

0.38

0.90

4.91

wPPAS

----------------------------QPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFA------FLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSVLKPELLTEIAGER---SARKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPT----------EYRYAHDEANAYAAEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPI

3pvsA

0.42

0.38

0.90

9.13

dPPAS2

----------------------------QPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRF------AFLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGQIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSVLKPELLTEIAGER---SARKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPT----------EYRYAHDEANAYAAEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPI

3pvsA

0.42

0.38

0.90

4.93

PPAS

----------------------------QPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFA------FLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSVLKPELLTEIAGER---SARKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPT----------EYRYAHDEANAYAAEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPI

3pvsA

0.42

0.38

0.90

9.84

Env-PPAS

----------------------------QPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFA------FLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGQIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSVLKPELLTEIAGER---SARKGDRFYDLISALHKSVRGSAPDAALYWYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIAQAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPT----------EYRYAHDEANAYAAEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPI

3u5zB

0.20

0.13

0.68

1.72

MUSTER

-------------------SMITVNEKEHILEQKYRPSTIDECILPAFDK---ETFKSITSKGKIPHIILHSPPGTGKTTVAKALCHDVNADMMFVNGSDCKIDFVRGPLTNFASAASDGRQKVIVIDEFDRSGAESQRHLRSFMEAYSSNCSIIITANNIDGIIKPLQSRCRVITFGQPTDEDKIEMMKQMIRRLTEICKGIAIDMKVVAALVKKNFPDFRKTIGELDSYS-----SKGVLDAGILSLVTNDRGAID----DVLESLKNKDVKQLRALDYSWFVGKLAEEIYSRVTPQSIIRMYEIVGENNQYHAANTELHLAYLFIQLACEMQWK-------------------------------------------------------------------------------------------------------------------

3pvsA1

0.41

0.20

0.49

2.69

dPPAS

----------------------------QPLAARMRPENLAQYIGQQHLLAAGKPLPRAIEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNRNAGRRTILFVDEVHRFA------FLPHIEDGTITFIGATTENPSFELNSALLSRARVYLLKSLSTEDIEQVLTQAMEDTRGYGGDIVLPDETRRAIAELVNGDARRALNTLEMMADMAEVDDSVLKPELLTEIAGERSAR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.66

Estimated TM-score = 0.80±0.09

Estimated RMSD = 5.7±3.6Å

Download Model 2

C-score=-0.49

Download Model 3

C-score=-0.73

Download Model 4

C-score=-2.15

Download Model 5

C-score=-2.44

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3pvsA	0.878	1.13	0.422	0.896
2	2v1uA	0.450	3.80	0.177	0.529
3	4wvyA	0.449	3.14	0.221	0.502
4	2c9oA	0.446	2.93	0.187	0.496
5	2qbyA	0.441	3.68	0.110	0.513
6	2qbyB	0.439	4.16	0.109	0.531
7	1iqpA	0.436	3.27	0.191	0.500
8	3cf1B	0.436	3.96	0.109	0.522
9	2chvA	0.434	2.37	0.188	0.471
10	2xszD	0.430	2.86	0.236	0.478

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.81	109	1sxjC	AGS	Rep, Mult	32,35,36,37,43,44,74,75,76,77,78,79,80,157,180,221,222,225
2	0.08	12	3glfC	BEF	Rep, Mult	74,75,78,79,129,222
3	0.04	8	2qbyB	MG	Rep, Mult	79,128,157
4	0.02	4	1hqcA	ADE	Rep, Mult	43,44,80,191,221,225
5	0.02	3	3glfD	UUU	Rep, Mult	146,167,171
6	0.01	2	3u60E	NUC	Rep, Mult	102,103,107
7	0.01	3	3u60B	NUC	Rep, Mult	102,103,107,132,134,136
8	0.00	1	2awb6	III	Rep, Mult	102,108,109,110,111,112,113,116,117
9	0.00	1	3u61B	NUC	Rep, Mult	133,134,282
10	0.00	1	3glfH	QNA	Rep, Mult	107,134,136

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.216	1ixsB	0.426	2.64	0.217	0.469	3.6.4.12	157,222
2	0.127	1in5A	0.423	2.60	0.208	0.465	3.6.4.12	157,222
3	0.060	1s1mB	0.357	6.55	0.052	0.549	6.3.4.2	NA
4	0.060	2vumB	0.355	7.42	0.036	0.600	2.7.7.6	295
5	0.060	3kx2B	0.352	6.56	0.035	0.533	3.6.4.13	75,79,131
6	0.060	3bq5A	0.350	6.57	0.033	0.533	2.1.1.14	NA
7	0.060	2pffB	0.365	6.65	0.044	0.557	2.3.1.86	NA
8	0.060	2vkzG	0.380	6.68	0.040	0.584	2.3.1.38,3.1.2.14	NA
9	0.060	2e1qA	0.355	7.55	0.039	0.620	1.17.3.2,1.17.1.4	NA
10	0.060	2pdaA	0.352	6.73	0.046	0.546	1.2.7.1	56
11	0.060	1bxrA	0.347	7.72	0.041	0.611	6.3.5.5	44
12	0.060	1eulA	0.306	7.38	0.046	0.520	3.6.3.8	NA
13	0.060	2uv8G	0.373	6.75	0.042	0.575	2.3.1.86	NA
14	0.060	3hmjA	0.379	7.08	0.047	0.620	2.3.1.86	NA
15	0.060	3b9jC	0.276	7.53	0.046	0.473	1.17.3.2,1.17.1.4	NA
16	0.060	3gtgB	0.357	7.41	0.038	0.602	2.7.7.6	89
17	0.060	1q2lA	0.361	7.23	0.036	0.597	3.4.24.55	NA
18	0.060	1t7lA	0.348	6.88	0.040	0.551	2.1.1.14	NA
19	0.060	3glfG	0.430	3.44	0.176	0.498	2.7.7.7	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.55	0.879	1.61	0.43	0.92	3pvsD
1	0.34	0.430	3.44	0.18	0.50	3glfG	GO:0000166 GO:0003677 GO:0003689 GO:0003887 GO:0005524 GO:0005663 GO:0006260 GO:0006261 GO:0009360 GO:0016740 GO:0016779 GO:0016887 GO:0017111 GO:0030337 GO:0042802 GO:0043846 GO:0071897
2	0.31	0.436	3.27	0.19	0.50	1iqpA	GO:0000166 GO:0003677 GO:0004519 GO:0005524 GO:0006260 GO:0006281 GO:0006310 GO:0009378 GO:0016539 GO:0032508 GO:0090305
3	0.31	0.441	3.68	0.11	0.51	2qbyA	GO:0000166 GO:0003677 GO:0005524 GO:0006260
4	0.27	0.412	3.03	0.16	0.46	3pfiA	GO:0000166 GO:0003677 GO:0004386 GO:0005524 GO:0006281 GO:0006310 GO:0006974 GO:0009378 GO:0009432 GO:0016787 GO:0032508
5	0.26	0.434	2.37	0.19	0.47	2chqA	GO:0000166 GO:0003677 GO:0003689 GO:0005524 GO:0005663 GO:0006260
6	0.26	0.423	2.60	0.21	0.46	1in5A	GO:0000166 GO:0003677 GO:0004386 GO:0005524 GO:0006281 GO:0006310 GO:0006974 GO:0009378 GO:0009432 GO:0016787 GO:0032508
7	0.26	0.426	2.64	0.22	0.47	1ixsB	GO:0000166 GO:0003677 GO:0004386 GO:0005524 GO:0006281 GO:0006310 GO:0006974 GO:0009378 GO:0009432 GO:0016787 GO:0032508
8	0.22	0.330	5.05	0.12	0.44	1g4aE	GO:0000166 GO:0005524 GO:0005737 GO:0005829 GO:0006508 GO:0009376 GO:0009408 GO:0016020 GO:0016887 GO:0042802 GO:0043335 GO:0070011
9	0.22	0.423	3.56	0.17	0.49	1e94E	GO:0000166 GO:0005524 GO:0005737 GO:0005829 GO:0006508 GO:0009376 GO:0009408 GO:0016020 GO:0016887 GO:0042802 GO:0043335 GO:0070011
10	0.14	0.407	3.18	0.13	0.47	3eihA	GO:0000166 GO:0005524 GO:0005634 GO:0005768 GO:0006810 GO:0007033 GO:0008568 GO:0010008 GO:0015031 GO:0016020 GO:0016125 GO:0016236 GO:0016887 GO:0031122 GO:0032511 GO:0042802 GO:0042803 GO:0045053 GO:0045324 GO:0051260 GO:0070676 GO:1990621
11	0.13	0.449	2.94	0.20	0.50	2xszC	GO:0000166 GO:0000812 GO:0003678 GO:0004386 GO:0005524 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005815 GO:0005856 GO:0005913 GO:0006281 GO:0006310 GO:0006351 GO:0006355 GO:0006357 GO:0006974 GO:0007049 GO:0007067 GO:0007283 GO:0016020 GO:0016363 GO:0016568 GO:0016787 GO:0016887 GO:0031011 GO:0032508 GO:0034080 GO:0035267 GO:0040008 GO:0043231 GO:0043967 GO:0043968 GO:0051301 GO:0070062 GO:0071339 GO:0097255 GO:0098609 GO:0098641 GO:1903146 GO:1903955 GO:1904837 GO:1904874
12	0.13	0.446	2.93	0.19	0.50	2c9oA	GO:0000166 GO:0000812 GO:0003678 GO:0004386 GO:0005524 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005815 GO:0005856 GO:0005913 GO:0006281 GO:0006310 GO:0006351 GO:0006355 GO:0006357 GO:0006974 GO:0007049 GO:0007067 GO:0007283 GO:0016020 GO:0016363 GO:0016568 GO:0016787 GO:0016887 GO:0031011 GO:0032508 GO:0034080 GO:0035267 GO:0040008 GO:0043231 GO:0043967 GO:0043968 GO:0051301 GO:0070062 GO:0071339 GO:0097255 GO:0098609 GO:0098641 GO:1903146 GO:1903955 GO:1904837 GO:1904874
13	0.12	0.414	3.08	0.19	0.47	3whkA	GO:0000166 GO:0000502 GO:0005524 GO:0005634 GO:0005737 GO:0006511 GO:0008540 GO:0010498 GO:0016787 GO:0016887 GO:0017025 GO:0022623 GO:0030163 GO:0030433 GO:0031595 GO:0031597 GO:0036402 GO:0043335 GO:0045899 GO:0070682 GO:1901800
14	0.11	0.413	3.77	0.10	0.49	3u5zB	GO:0000166 GO:0003677 GO:0005524 GO:0006260 GO:0039686
15	0.11	0.415	3.39	0.14	0.47	1sxjB	GO:0000166 GO:0003677 GO:0003689 GO:0005524 GO:0005634 GO:0005663 GO:0005829 GO:0006260 GO:0006272 GO:0006298 GO:0007049 GO:0007062 GO:0031389 GO:0031390 GO:0031391
16	0.09	0.420	4.15	0.10	0.52	4xgcD	GO:0000808 GO:0003677 GO:0003688 GO:0005634 GO:0005664 GO:0006260 GO:0006270
17	0.08	0.421	3.60	0.17	0.49	1im2A	GO:0000166 GO:0005524 GO:0005737 GO:0006508 GO:0009376 GO:0016887 GO:0043335 GO:0070011
18	0.07	0.447	3.34	0.20	0.51	4ww4B	GO:0000166 GO:0003678 GO:0004386 GO:0005524 GO:0005634 GO:0006281 GO:0006351 GO:0006355 GO:0006974 GO:0016568 GO:0016787 GO:0032508 GO:0043141

Consensus prediction of GO terms

Molecular Function	GO:0005524	GO:0009378	GO:0003689	GO:0042802	GO:0003887	GO:0030337	GO:0004519
GO-Score	0.77	0.50	0.34	0.34	0.34	0.34	0.31
Biological Processes	GO:0031668	GO:0006281	GO:0032508	GO:0006310	GO:0006261	GO:0071897	GO:0016539	GO:0090305
GO-Score	0.54	0.50	0.50	0.50	0.34	0.34	0.31	0.31
Cellular Component	GO:0043846	GO:0005663
GO-Score	0.34	0.34

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.