I-TASSER results

I-TASSER results for job id Rv2525c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (240 residues)
MSVSRRDVLKFAAATPGVLGLGVVASSLRAAPASAGSLGTLLDYAAGVIPASQIRAAGAV
GAIRYVSDRRPGGAWMLGKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQH
ARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKT
IDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MSVSRRDVLKFAAATPGVLGLGVVASSLRAAPASAGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWMLGKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA
Prediction	CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEHCCCCCCCHHHHHHHCCCEEEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHCCHHHHHHHHHHCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHCCCHCCCCCCCCC
Conf.Score	987898988765555455555555554567778888776677667899999999987985999985899999888778889999999999989989998578999865554768999999999999999989999968999985689977878889999999999985766589869899999999886467887668899987676544444445555678689855443457899899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MSVSRRDVLKFAAATPGVLGLGVVASSLRAAPASAGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWMLGKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA
Prediction	652433400411233312223222233343443443410200000343241630252203000000034444464345150335105302723020000001346433203401630241044015203726137610000000000347405520240040025004532000001240032026430130000011234323421302022243534413103002030137322117
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEHCCCCCCCHHHHHHHCCCEEEEHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHCCHHHHHHHHHHCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHCCCHCCCCCCCCC
MSVSRRDVLKFAAATPGVLGLGVVASSLRAAPASAGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWMLGKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

4pmnA

0.97

0.85

0.87

3.10

MUSTER

------------------------------LYFQGGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWL-GKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

4pmnA

0.97

0.85

0.87

6.00

dPPAS

------------------------------LYFQGGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWL-GKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

4pmnA

0.97

0.85

0.87

6.64

wdPPAS

----------------------------LYFQG--GSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWL-GKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

4pmnA

0.99

0.85

0.86

3.72

wMUSTER

---------------------------------QGGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWL-GKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

4pmnA

0.96

0.84

0.87

6.19

wPPAS

----------------------------LYFQGGS--LGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWL-GKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

4pmnA

0.97

0.85

0.87

14.00

dPPAS2

------------------------------LYFQGGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWL-GKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

4pmnA

0.97

0.85

0.87

5.03

PPAS

------------------------------LYFQGGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWL-GKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

4pmnA

0.97

0.85

0.87

6.97

Env-PPAS

------------------------------LYFQGGSLGTLLDYAAGVIPASQIRAAGAVGAIRYVSDRRPGGAWL-GKPIQLSEARDLSGNGLKIVSCYQYGKGSTADWLGGASAGVQHARRGSELHAAAGGPTSAPIYASIDDNPSYEQYKNQIVPYLRSWESVIGHQRTGVYANSKTIDWAVNDGLGSYFWQHNWGSPKGYTHPAAHLHQVEIDKRKVGGVGVDVNQILKPQFGQWA

1sfsA

0.18

0.14

0.77

0.98

MUSTER

-------------------------------------GIWGVD-SAQVVTDQLFQCV-PKFWGRYLSEVPN-----VSEGLTRDEIVRIRNYGVKVLPIYNAFREAV-----GYANGQVAARNAVFHARRLGIPKNKLLFANIEDFFAVD--AAWIAAWVETLYPT---YRPGLYADAAAYCEAVSRNVQAVIWSAAPRPGTTKEAPRYQPAAPPCSARDAEVCPVDTNLADRRDF-LY-

1sfsA

0.14

0.11

0.78

1.46

dPPAS

-------------------------------------GIWGVDSAQVVTDQCVRTELGYKFWGRYLSEVPNVSEG-----LTRDEIVRIRNYGVKVLPIYNAFREAV-----GYANGQVAARNAVFHARRLGIPKNKLLFANIEDFFAVD--AAWIAAWVETLYPTG--YRPGLYADAAYCEAVSRNQVAVQAVIWSAAPRPGTTKEQKAP-RYQPAAPPCSANVWVWQYGRDAEVCPVD

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-0.09

Estimated TM-score = 0.70±0.12

Estimated RMSD = 5.9±3.7Å

Download Model 2

C-score=-1.12

Download Model 3

C-score=-0.79

Download Model 4

C-score=-0.53

Download Model 5

C-score=-3.43

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4pmnA	0.860	0.65	0.976	0.871
2	2ww5A	0.648	4.06	0.129	0.846
3	1sfsA	0.644	2.83	0.174	0.754
4	5jipA	0.644	3.92	0.094	0.829
5	1uz4A	0.622	4.12	0.076	0.833
6	2wagA	0.620	4.27	0.091	0.821
7	4krtA	0.618	4.07	0.091	0.800
8	4ff5A	0.614	3.45	0.116	0.767
9	5a6sA	0.613	3.55	0.137	0.758
10	1jfxA	0.609	3.94	0.164	0.783

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.17	4	4pmrA	EPE	Rep, Mult	70,79,101,144,175,213,227
2	0.06	2	2gk1A	UUU	Rep, Mult	62,64,139
3	0.03	1	2ddsC	CO	Rep, Mult	144,194
4	0.03	1	4ea2A	MG	Rep, Mult	43,159
5	0.03	1	3d6aA	MG	Rep, Mult	148,149
6	0.03	1	3c9zA	UUU	Rep, Mult	92,144
7	0.03	1	2q7dA	MN	Rep, Mult	144,146
8	0.03	1	2buqA	CAQ	Rep, Mult	158,159
9	0.03	1	2gq9A	HBA	Rep, Mult	98,137,138,139
10	0.03	1	3lkvA	ZN	Rep, Mult	129,136,172
11	0.03	1	2gk1A	NGT	Rep, Mult	144,145,175,196,218,227
12	0.03	1	1tq2B	MG	Rep, Mult	178,227
13	0.03	1	2wagA	MG	Rep, Mult	152,156,157,161,162,187,189
14	0.03	1	2wwdA	UUU	Rep, Mult	175,177,178,179,196,197

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.066	1n7kA	0.521	4.10	0.082	0.683	4.1.2.4	NA
2	0.060	1jfxA	0.609	3.94	0.164	0.783	3.2.1.17	100,175,198,227
3	0.060	1myrA	0.583	4.28	0.055	0.787	3.2.1.147	NA
4	0.060	3f4vA	0.582	4.07	0.080	0.771	3.2.1.21	NA
5	0.060	1kbiA	0.579	4.53	0.047	0.812	1.1.2.3	44,210
6	0.060	2tmdA	0.585	4.09	0.081	0.787	1.5.8.2	61
7	0.060	1c7sA	0.611	4.30	0.042	0.838	3.2.1.52	NA
8	0.060	1fa2A	0.596	4.28	0.069	0.812	3.2.1.2	NA
9	0.060	2dgaA	0.585	4.29	0.065	0.792	3.2.1.21	146,149
10	0.060	1kfwA	0.594	4.32	0.081	0.838	3.2.1.14	NA
11	0.060	1h4pA	0.580	4.39	0.071	0.796	3.2.1.58	NA
12	0.060	1hp4A	0.581	4.22	0.082	0.792	3.2.1.52	NA
13	0.060	1cz1A	0.582	4.40	0.034	0.796	3.2.1.58	NA
14	0.060	1j5sA	0.594	4.82	0.076	0.879	5.3.1.12	198,200,206
15	0.060	2oykB	0.584	4.52	0.053	0.796	3.2.1.123	64,196
16	0.060	2gjxA	0.589	4.10	0.060	0.792	3.2.1.52	NA
17	0.060	1b1yA	0.598	4.31	0.069	0.812	3.2.1.2	NA
18	0.060	2whjA	0.604	4.17	0.099	0.796	3.2.1.78	NA
19	0.060	1djnA	0.584	4.09	0.081	0.787	1.5.8.2,1.5.99.7	140
20	0.060	2jepA	0.580	4.01	0.082	0.771	3.2.1.151	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.67	0.865	0.76	0.97	0.88	4pmoA	GO:0005576 GO:0005975 GO:0008610 GO:0046677
1	0.25	0.644	2.83	0.17	0.75	1sfsA	GO:0005975
2	0.18	0.614	3.45	0.12	0.77	4ff5A	GO:0003796 GO:0005975 GO:0009253 GO:0016020 GO:0016021 GO:0016998
3	0.18	0.574	3.87	0.11	0.74	3hmcA	GO:0003796 GO:0005975 GO:0009253 GO:0016787 GO:0016998
4	0.17	0.568	3.73	0.09	0.72	1h09A	GO:0003796 GO:0003824 GO:0005975 GO:0008152 GO:0009253 GO:0016787 GO:0016798 GO:0016998 GO:0019835 GO:0042742 GO:0044659
5	0.17	0.592	3.83	0.13	0.75	2x8rB	GO:0003796 GO:0005975 GO:0009253 GO:0016787 GO:0016998
6	0.14	0.613	3.55	0.14	0.76	5a6sA	GO:0003796 GO:0005975 GO:0009253 GO:0016998
7	0.14	0.583	3.96	0.12	0.77	4jz5A	GO:0003796 GO:0005975 GO:0009253 GO:0016998
8	0.12	0.620	4.27	0.09	0.82	2wagA	GO:0003796 GO:0005975 GO:0009253 GO:0016020 GO:0016021 GO:0016787 GO:0016998 GO:0046872
9	0.10	0.618	4.07	0.09	0.80	4krtA	GO:0003796 GO:0005975 GO:0009253 GO:0016998
10	0.09	0.649	4.10	0.12	0.85	2wwdA	GO:0003796 GO:0005975 GO:0008152 GO:0009253 GO:0016787 GO:0016798 GO:0016998
11	0.07	0.623	4.11	0.08	0.83	1uuqA	GO:0005975
12	0.07	0.609	3.94	0.16	0.78	1jfxA	GO:0003796 GO:0005576 GO:0005615 GO:0005975 GO:0008152 GO:0009253 GO:0016787 GO:0016798 GO:0016998
13	0.07	0.500	4.85	0.04	0.72	1rh9A	GO:0004553 GO:0005576 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0016985 GO:0046355
14	0.07	0.577	3.59	0.12	0.73	2nw0A
15	0.07	0.612	4.01	0.07	0.81	3pznA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0016985
16	0.07	0.543	4.86	0.06	0.78	4qp0A	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0016985
17	0.07	0.537	4.99	0.05	0.79	4aweA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0016985
18	0.07	0.603	4.01	0.07	0.80	1qnoA	GO:0004553 GO:0005576 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030248
19	0.07	0.605	4.22	0.03	0.82	3wh9A	GO:0004553 GO:0005576 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0016985 GO:0046355
20	0.07	0.602	4.27	0.07	0.82	3zizA	GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0016985
21	0.07	0.506	5.02	0.05	0.77	4mupB	GO:0016787 GO:0016853
22	0.07	0.558	4.46	0.05	0.75	3lpfA	GO:0004553 GO:0004566 GO:0005829 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0019391 GO:0043231
23	0.06	0.414	5.57	0.08	0.66	4ls8A	GO:0003824 GO:0006629 GO:0006631 GO:0006633 GO:0008152 GO:0016740 GO:0016746 GO:0016747 GO:0033817
24	0.06	0.403	5.72	0.05	0.67	3o04A	GO:0003824 GO:0006633 GO:0008152 GO:0016740 GO:0016746 GO:0016747 GO:0033817

Consensus prediction of GO terms

Molecular Function	GO:0003796
GO-Score	0.45
Biological Processes	GO:0005975	GO:0046677	GO:0008610	GO:0016998	GO:0009253	GO:0019076	GO:0009617	GO:0044661	GO:0098542	GO:0001897	GO:0039633
GO-Score	0.86	0.67	0.67	0.45	0.45	0.35	0.35	0.35	0.35	0.35	0.35
Cellular Component	GO:0005576
GO-Score	0.67

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.