I-TASSER results

I-TASSER results for job id Rv2519

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (492 residues)
MSRLIVAPDWLASAAAEVQSIGSALSAANAAAAAPTTLLVAAAEDEVSAAAAALFANYGR
EYQTLSVRFASLDQQFAQALNSAAASYQTAEATGASLVQTATQGVLGVINAPTEFMFGRS
LIGDGADGTAASPIGEPGGILYGDGGNGYSQTTPGAVGGAGGSAGFIGNGGAGGAGGPGA
GGGTGGLGGWLWGNNGAAGTGDPVNVAVPLRVENNFPLVNLLVNRGPTVPILLDTGSSSL
VIPFWKIGWQNLGLPTGFDVVHYGNGVSIVYADVPTTVDFGGGAATTPTSVHVGILPYPR
NLDSLVLIASGGAFGPNGNGILGIGPNVGSYAVSGPGNVVTTDLPGQLNEGTLIDIPGGY
MQFGPNTGTPITSVTGAPITVLNVQIGGYDPNGGYWSLPSIFDSGGNHGTLPAVILGTGQ
TTGYAPPGTVISISIHDNQTLLYQYTTTASNSPVVTADPRLNTGLTPFLLGPVYISNNPS
GVGTVVFNYPPP

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSRLIVAPDWLASAAAEVQSIGSALSAANAAAAAPTTLLVAAAEDEVSAAAAALFANYGREYQTLSVRFASLDQQFAQALNSAAASYQTAEATGASLVQTATQGVLGVINAPTEFMFGRSLIGDGADGTAASPIGEPGGILYGDGGNGYSQTTPGAVGGAGGSAGFIGNGGAGGAGGPGAGGGTGGLGGWLWGNNGAAGTGDPVNVAVPLRVENNFPLVNLLVNRGPTVPILLDTGSSSLVIPFWKIGWQNLGLPTGFDVVHYGNGVSIVYADVPTTVDFGGGAATTPTSVHVGILPYPRNLDSLVLIASGGAFGPNGNGILGIGPNVGSYAVSGPGNVVTTDLPGQLNEGTLIDIPGGYMQFGPNTGTPITSVTGAPITVLNVQIGGYDPNGGYWSLPSIFDSGGNHGTLPAVILGTGQTTGYAPPGTVISISIHDNQTLLYQYTTTASNSPVVTADPRLNTGLTPFLLGPVYISNNPSGVGTVVFNYPPP
Prediction	CCEEHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHCCCCCEEEEEHCCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHCCCEEHCCCCCCCCCCCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCCEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHCCCCEEEHCCCCCCCCEHCCCCCCEEEEEEHCCCCCCCCCCCCCCEHCCCCCCCCCCCCHCCCCCCCCCCCCCCEEEEEHCCCCEEEEEEEHCCCCCCEEHCCCCCCCCHHHHHHCCEEEHCCCCCCCEEEHCCCCC
Conf.Score	977873569999999999999999999887655555434566666568999999999999999999999999999999999877765456554556788877776567888656546888888888888889998888746578989888899988888887777778888888888888888888887777778888888776657889976898789999778875568875677666667666765446788888777889876556655576576588877888888757765888888877777777766788757876678888888898887655688877887576567875775788889855558887566788876778888765677566688888888764457788887778986799996799668999985889986787688887645555548777766899886577668999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSRLIVAPDWLASAAAEVQSIGSALSAANAAAAAPTTLLVAAAEDEVSAAAAALFANYGREYQTLSVRFASLDQQFAQALNSAAASYQTAEATGASLVQTATQGVLGVINAPTEFMFGRSLIGDGADGTAASPIGEPGGILYGDGGNGYSQTTPGAVGGAGGSAGFIGNGGAGGAGGPGAGGGTGGLGGWLWGNNGAAGTGDPVNVAVPLRVENNFPLVNLLVNRGPTVPILLDTGSSSLVIPFWKIGWQNLGLPTGFDVVHYGNGVSIVYADVPTTVDFGGGAATTPTSVHVGILPYPRNLDSLVLIASGGAFGPNGNGILGIGPNVGSYAVSGPGNVVTTDLPGQLNEGTLIDIPGGYMQFGPNTGTPITSVTGAPITVLNVQIGGYDPNGGYWSLPSIFDSGGNHGTLPAVILGTGQTTGYAPPGTVISISIHDNQTLLYQYTTTASNSPVVTADPRLNTGLTPFLLGPVYISNNPSGVGTVVFNYPPP
Prediction	311000001201000320330220031010000000000023232100000000003004201100200020021003001100201122322012213212320131343222200111012433423243342210000000111123332112221221200122331111222443433221110112233223344332423021304342010100032122010102210010002133111120232322221311210111214130103001111112230200102024332222122233312430200000001121212325331012202240331110213412020122413241203001101020101223451212313020012123010110001223231201240201020355220111102344431302353200000100120000001114100102032448
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCEEHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHCCCCCEEEEEHCCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHCCCEEHCCCCCCCCCCCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCCEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHCCCCEEEHCCCCCCCCEHCCCCCCEEEEEEHCCCCCCCCCCCCCCEHCCCCCCCCCCCCHCCCCCCCCCCCCCCEEEEEHCCCCEEEEEEEHCCCCCCEEHCCCCCCCCHHHHHHCCEEEHCCCCCCCEEEHCCCCC
MSRLIVAPDWLASAAAEVQSIGSALSAANAAAAAPTTLLVAAAEDEVSAAAAALFANYGREYQTLSVRFASLDQQFAQALNSAAASYQTAEATGASLVQTATQGVLGVINAPTEFMFGRSLIGDGADGTAASPIGEPGGILYGDGGNGYSQTTPGAVGGAGGSAGFIGNGGAGGAGGPGAGGGTGGLGGWLWGNNGAAGTGDPVNVAVPLRVENNFPLVNLLVNRGPTVPILLDTGSSSLVIPFWKIGWQNLGLPTGFDVVHYGNGVSIVYADVPTTVDFGGGAATTPTSVHVGILPYPRNLDSLVLIASGGAFGPNGNGILGIGPNVGSYAVSGPGNVVTTDLPGQLNEGTLIDIPGGYMQFGPNTGTPITSVTGAPITVLNVQIGGYDPNGGYWSLPSIFDSGGNHGTLPAVILGTGQTTGYAPPGTVISISIHDNQTLLYQYTTTASNSPVVTADPRLNTGLTPFLLGPVYISNNPSGVGTVVFNYPPP

4ehcA

0.41

0.23

0.56

1.36

MUSTER

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSSHHHHHHSSGTLTNATVPLQLVNTTEPVVFISLNGGQVPVLLDTGSTGLVDS--QFLTQNFGPVIGTGTAGYAGGLTYNYNTYSTTVDFGNGLLTLPTSVNVVTSSSPGTLGNFLSRS-------GAVGVLGIGPNNG-----FPGTSSIVTAPGLLNNGVLIDESAGILQFGPNTLTGGITISGAPISTVAVQIDNG-----PLQQAPVFDSGGINGTIPSA-LASLPSGGFVPAGTTISVYTSDGQTLLYSYTTTATNTPFVTSGG-VNTGHVPFAQQPIYVSYSPTIGTTT-------

4kh3A

0.14

0.13

0.94

1.09

MUSTER

-ADIVVHP---TVNGGTLVNHDNQFVSGTANGVTVSTGLELGPDSDENTGGQWIKGGTGRNTTVTAN---------RQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAESSGQMVGGTAESTTINKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRL---EGGNQYVHNGGTATETLINRDGWQVIKEGGTAAHTTINQKGKLQVNAGG-KASDVTQNTGGALVTSTAATVTGTNRLGAFSVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDIRNGGAATTVSMGNGGVLLADSG--AAVSGTRSDGKAFSIGGGQADALMLEKGS---SFTLNAGDTATDTTVNGGLFTARGGTLAGTT--TLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTSGSGTLTVSNTTLTQKAVNLNEGTLTLNDSTVTTDVIAQ----R

4ehcA

0.41

0.23

0.56

1.88

dPPAS

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSSHHHHHHSSGTLTNATVPLQLVNTTEPVVFISLNGGQVPVLLDTGSTGLVDSQFLT--QNFGPVIGTGTAGYAGGLTYNYNTYSTTVDFGNGLLTLPTSVNVVTSSSPGTLGNFLSR-------SGAVGVLGIGPNNGFPGTSSI-----VTAPGLLNNGVLIDESAGILQFGPNTLTGGITISGAPISTVAVQIDNG-----PLQQAPVFDSGGINGTIPSA-LASLPSGGFVPAGTTISVYTSDGQTLLYSYTTTATNTPFVTSG-GVNTGHVPFAQQPIYVSYSPTIGTTT-------

5gaoE

0.09

0.07

0.69

1.02

dPPAS

--------------------------------------------------------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-----AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---------AAAAAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK---------------------------------------------------------------------

4ehcA

0.40

0.23

0.56

2.57

wdPPAS

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSSHHHHHHSSGTLTNATVPLQLVNTTEPVVFISLNGGQVPVLLDTGSTGLVDSQFLTQ--NFGPVIGTGTAGYAGGLTYNYNTYSTTVDFGNGLLTLPTSVNVVTSSSPGTLGNFLSR-------SGAVGVLGIGPNNGFPGTSSI-----VTAPGLLNNGVLIDESAGILQFGPNTLTGGITISGAPISTVAVQIDNG-----PLQQAPVFDSGGINGTIPSA-LASLPSGGFVPAGTTISVYTSDGQTLLYSYTTTATNTPFVTSG-GVNTGHVPFAQQPIYVSYSPTIGTTT-------

4ut1A

0.13

0.94

1.08

wMUSTER

GSSLSTYYTLVAQLNNYVAGIATAITNYFTGLQTVANNAADPSA-SNAQTLASQLVAAGQQYSQLRQSVNSQLTDTVTQINSYTSQIAQASASSPNQLLDQRDLAVSLAGNYSVFLSGQPLVGNASYQLATVASPSDPSELTIVSKGVAGSAQPGPTQYLPDVSLTGGALGGLLAFRSQTLDPAQALGVDMSGNPGGSLFAVGAPAVYANQNNTGSATLSVSFVDGT------QPTTSDYALSYDGAKYTLTDRATGSVVGTAPSSTPPTMTIGGLKLSLS---STPNAGDSFTVLPTRGALDGFSLATAGSAIAAASPVLAAGVATNSGTGVISQGSVSAGYQLPSGTTTLAYNAASKTLSGFPVGTT--VTIAGTPPTSINITSATTPVPYDPKGASMTISSTTQPAPSGVMNGVSVSLSGTPADGDQFTIGANKGTN-------DGRNALALSQSKTMNNGTTTLTG---AYAGYVNAIGNAA------

4ehcA

0.42

0.23

0.56

1.53

wMUSTER

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SSHHHHHHSSGTLTNATVPLQLVNTTEPVVFISLNGGQVPVLLDTGSTGLVDS--QFLTQNFGPVIGTGTAGYAGGLTYNYNTYSTTVDFGNGLLTLPTSVNVVTSSSPGTLGNFLSR-------SGAVGVLGIGPNNG-----FPGTSSIVTAPGLLNNGVLIDESAGILQFGPNTLTGGITISGAPISTVAVQID-----NGPLQQAPVFDSGGINGTIPSA-LASLPSGGFVPAGTTISVYTSDGQTLLYSYTTTATNTPFVTSG-GVNTGHVPFAQQPIYVSYSPTIGTTT-------

5gaoE

0.09

0.07

0.69

1.53

dPPAS2

--------------------------------------------------------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-----AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---------AAAAAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK---------------------------------------------------------------------

4ehcA

0.42

0.23

0.55

3.59

wPPAS

------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSSHHHSSGTLTNATVPLQLVNTTPVVFISLNGGQV-PVLLDTGSTGLVDSQFLTQ--NFGPVIGTGTAGYAGGLTYNYNTYSTTVDFGNGLLTLPTSVNVVTSSSPGTLGNFLSR-------SGAVGVLGIGPNNGFPGTSSI-----VTAPGLLNNGVLIDESAGILQFGPNTLTGGITISGAPISTVAVQIDNG-----PLQQAPVFDSGGINGTIPSA-LASLPSGGFVPAGTTISVYTSDGQTLLYSYTTTATNTPFVTSG-GVNTGHVPFAQQPIYVSYSPTIGTTT-------

5gaoE

0.14

0.10

0.69

1.63

PPAS

------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA------AAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK----------------------------------------------------------------------------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.29

Estimated TM-score = 0.44±0.14

Estimated RMSD = 12.8±4.2Å

Download Model 2

C-score=-2.08

Download Model 3

C-score=-2.66

Download Model 4

C-score=-3.28

Download Model 5

C-score=-2.90

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4ut1A	0.733	4.22	0.096	0.864
2	2d4yA	0.503	4.68	0.084	0.624
3	5d06A	0.396	7.53	0.043	0.656
4	4pkcA	0.372	8.01	0.065	0.646
5	5i2aA1	0.371	7.97	0.043	0.648
6	5a0uA	0.370	7.79	0.040	0.638
7	4r04A	0.369	7.68	0.057	0.626
8	2f3oA	0.369	7.98	0.053	0.652
9	5dotA	0.367	7.42	0.035	0.604
10	1r8wA	0.367	7.87	0.035	0.636

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.16	8	3jcuX	CLA	Rep, Mult	55,59
2	0.04	2	4zjbG	PNS	Rep, Mult	71,72
3	0.04	2	4rku4	CLA	Rep, Mult	56,57
4	0.04	2	2y00A	2CV	Rep, Mult	24,27,47,54
5	0.02	1	3hw1A	EV2	Rep, Mult	234,236,263,321,403
6	0.02	1	2dwhA	UUU	Rep, Mult	61,64,65,68
7	0.02	1	3hvgB	EV0	Rep, Mult	234,263,403
8	0.02	1	1ibqA	ZN	Rep, Mult	234,403
9	0.02	1	1pprM	PID	Rep, Mult	11,15
10	0.02	1	5e1jA	CA	Rep, Mult	13,17,61
11	0.02	1	3pp5A	CA	Rep, Mult	44,45
12	0.02	1	1tqqA	NCO	Rep, Mult	5,9
13	0.02	1	3c9iB	XE	Rep, Mult	18,21
14	0.02	1	2px1A	UUU	Rep, Mult	50,53,54,57
15	0.02	1	3hykA	MG	Rep, Mult	45,91

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2c4mC	0.311	7.42	0.055	0.520	2.4.1.1	NA
2	0.060	2je8B	0.312	7.34	0.045	0.504	3.2.1.25	NA
3	0.060	1yq2A	0.328	7.34	0.028	0.541	3.2.1.23	17,61
4	0.060	3k1dA	0.314	7.57	0.050	0.530	2.4.1.18	185
5	0.060	2vuaA	0.243	7.14	0.035	0.390	3.4.24.69	10
6	0.060	1h7aA	0.318	7.41	0.039	0.514	1.17.4.2	NA
7	0.060	3fwlA	0.324	7.41	0.044	0.528	2.4.1.129	NA
8	0.060	3ffzA	0.335	7.64	0.043	0.571	3.4.24.69	NA
9	0.060	2zzgA	0.336	7.62	0.041	0.573	6.1.1.7	NA
10	0.060	1e1yA	0.317	7.37	0.064	0.518	2.4.1.1	NA
11	0.060	3btaA	0.314	7.80	0.029	0.545	3.4.24.69	NA
12	0.060	1gl9B	0.288	6.86	0.045	0.447	5.99.1.3	NA
13	0.060	2pffB	0.318	7.74	0.086	0.543	2.3.1.86	181,183
14	0.060	3g61A	0.336	7.13	0.044	0.516	3.6.3.44	NA
15	0.060	2jjdF	0.316	7.45	0.052	0.526	3.1.3.48	NA
16	0.060	1dgjA	0.323	7.00	0.055	0.506	1.2.-.-	NA
17	0.060	3hmjA	0.314	7.40	0.028	0.510	2.3.1.86	NA
18	0.060	2agsA	0.316	7.01	0.048	0.496	3.2.1.18	130
19	0.060	3b8cB	0.329	7.30	0.064	0.537	3.6.3.6	14,181

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.13	0.733	4.22	0.10	0.86	4ut1A	GO:0005198 GO:0009424 GO:0044780
1	0.07	0.503	4.68	0.08	0.62	2d4yA	GO:0005198 GO:0005576 GO:0009288 GO:0009424 GO:0044780 GO:0071973
2	0.06	0.368	7.86	0.05	0.64	5i2aB	GO:0003824 GO:0008152
3	0.06	0.369	7.92	0.04	0.64	5i2gA	GO:0003824 GO:0008152
4	0.06	0.368	7.86	0.03	0.64	2yajC	GO:0003824 GO:0008152 GO:0016829 GO:0043722
5	0.06	0.368	7.93	0.03	0.64	4mtjA	GO:0003824 GO:0008152
6	0.06	0.335	7.56	0.04	0.56	5bweA	GO:0003824 GO:0008152
7	0.06	0.369	7.98	0.05	0.65	2f3oA	GO:0003824 GO:0008152 GO:0016829
8	0.06	0.363	7.64	0.05	0.63	1cm5A	GO:0003824 GO:0005737 GO:0005829 GO:0005975 GO:0006006 GO:0006567 GO:0008152 GO:0008861 GO:0016020 GO:0016740 GO:0016746
9	0.06	0.276	7.49	0.04	0.46	1rqgA	GO:0000049 GO:0000166 GO:0003723 GO:0004812 GO:0004825 GO:0005524 GO:0005737 GO:0006412 GO:0006418 GO:0006431 GO:0016874 GO:0046872
10	0.06	0.255	7.56	0.03	0.43	2pffA	GO:0000287 GO:0003824 GO:0004312 GO:0004315 GO:0004316 GO:0004321 GO:0005829 GO:0005835 GO:0006629 GO:0006631 GO:0006633 GO:0008152 GO:0008897 GO:0016491 GO:0016740 GO:0042759 GO:0046872 GO:0055114 GO:0102132
11	0.06	0.304	7.53	0.05	0.51	3ueqA	GO:0003824 GO:0005576 GO:0005975 GO:0016740 GO:0016757 GO:0047669
12	0.06	0.264	8.06	0.05	0.48	4aieA	GO:0003824 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0043896 GO:0046872
13	0.06	0.239	7.92	0.06	0.42	2wssA	GO:0000166 GO:0005524 GO:0005739 GO:0005743 GO:0005753 GO:0005886 GO:0006754 GO:0006810 GO:0006811 GO:0015986 GO:0015991 GO:0015992 GO:0016020 GO:0016820 GO:0033178 GO:0045261 GO:0046034 GO:0046933 GO:0046961
14	0.06	0.260	7.15	0.04	0.42	3dhuA	GO:0003824 GO:0004556 GO:0005975 GO:0008152 GO:0016787 GO:0016798
15	0.06	0.246	7.54	0.03	0.41	2qa1A	GO:0000166 GO:0016491 GO:0055114 GO:0071949
16	0.06	0.245	6.74	0.04	0.37	3aw5A	GO:0005507 GO:0016020 GO:0016021 GO:0016491 GO:0016722 GO:0046872 GO:0055114
17	0.06	0.244	7.14	0.05	0.39	4icyA	GO:0000166 GO:0016491 GO:0055114 GO:0071949
18	0.06	0.251	6.58	0.06	0.39	4gklA	GO:0003824 GO:0005975

Consensus prediction of GO terms

Molecular Function	GO:0005198	GO:0043722
GO-Score	0.19	0.06
Biological Processes	GO:0030031	GO:0044781	GO:0070925
GO-Score	0.38	0.38	0.38
Cellular Component	GO:0044461
GO-Score	0.38

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.