I-TASSER results

I-TASSER results for job id Rv2510c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (533 residues)
MGTESAAGGPGGPAQRIAAGYTVEGQALQLGTVVVDGEPDPSAQIRIPLATVNRHGLVAG
ATGTGKTKTLQLIAEQLSAAGVAVLMADVKGDLSGLARPGEAADKTAARAKDTGDDWVPT
AFPVEFLSLGASGVGVPVRATISSFGPILLAKVLGLNATQESTLGLIFHWADQRGLPLLD
LKDLRAVITHLTSDEGKVELKSLGAVSPTTAGVILRALVNLEAEGADTFFGEPELRPEDL
LRVDSQGRGIISLLEFGSQALRPAMFSTFLMWVLADLFTFLPEVGDLDKPKLVFFFDEAH
LLFTDASKAFLEQVEQTVKLIRSKGVGVFFCTQLPTDLPNDVLSQLGARIQHALRAFTPD
DHKALRKTVRTYPKTDVYDLESALTSLGTGEAVVTVLSEKGAPTPVAWTRMRAPRSLMAA
IGAEAIGAAAQASSLQAVYGQTIDRPSAHEILSAKLAPAQEAPAQEAPAPRGQYDPLPWP
DDFEVPPMPAPVEPQGPAVWEEILKNPTVKSVLNTTAREITRSIFGTGRRRRK

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MGTESAAGGPGGPAQRIAAGYTVEGQALQLGTVVVDGEPDPSAQIRIPLATVNRHGLVAGATGTGKTKTLQLIAEQLSAAGVAVLMADVKGDLSGLARPGEAADKTAARAKDTGDDWVPTAFPVEFLSLGASGVGVPVRATISSFGPILLAKVLGLNATQESTLGLIFHWADQRGLPLLDLKDLRAVITHLTSDEGKVELKSLGAVSPTTAGVILRALVNLEAEGADTFFGEPELRPEDLLRVDSQGRGIISLLEFGSQALRPAMFSTFLMWVLADLFTFLPEVGDLDKPKLVFFFDEAHLLFTDASKAFLEQVEQTVKLIRSKGVGVFFCTQLPTDLPNDVLSQLGARIQHALRAFTPDDHKALRKTVRTYPKTDVYDLESALTSLGTGEAVVTVLSEKGAPTPVAWTRMRAPRSLMAAIGAEAIGAAAQASSLQAVYGQTIDRPSAHEILSAKLAPAQEAPAQEAPAPRGQYDPLPWPDDFEVPPMPAPVEPQGPAVWEEILKNPTVKSVLNTTAREITRSIFGTGRRRRK
Prediction	CCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEEHCCCCCCCCEEEHCHHHHHHHEEEHCCCCCCHHHHHHHHHHHHHHHCCCEEEEHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEEHCCCCCCCCCEEEEEHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHCHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEHCCCCCCCHHHHHHHCCHHHHEHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEHCCCCCCCEEEEEEEHCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
Conf.Score	99888888888757887766778888488657997887788857987898855445776799998999999999999998998999986986787765678875699999998998876679789986888888855554468876688999879977899999999999988787557899999999999865556678887798878999999999999984865446888888778543578998589999857886668999999999999999997986888888689998446887789986999999999999987596999998898769989999876555543447999999999999997677776568999876899799999768888875678887768877778999999999999897677655557757799999998777666666655556655555566666666677788887776579999999999999999999999998567787889
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MGTESAAGGPGGPAQRIAAGYTVEGQALQLGTVVVDGEPDPSAQIRIPLATVNRHGLVAGATGTGKTKTLQLIAEQLSAAGVAVLMADVKGDLSGLARPGEAADKTAARAKDTGDDWVPTAFPVEFLSLGASGVGVPVRATISSFGPILLAKVLGLNATQESTLGLIFHWADQRGLPLLDLKDLRAVITHLTSDEGKVELKSLGAVSPTTAGVILRALVNLEAEGADTFFGEPELRPEDLLRVDSQGRGIISLLEFGSQALRPAMFSTFLMWVLADLFTFLPEVGDLDKPKLVFFFDEAHLLFTDASKAFLEQVEQTVKLIRSKGVGVFFCTQLPTDLPNDVLSQLGARIQHALRAFTPDDHKALRKTVRTYPKTDVYDLESALTSLGTGEAVVTVLSEKGAPTPVAWTRMRAPRSLMAAIGAEAIGAAAQASSLQAVYGQTIDRPSAHEILSAKLAPAQEAPAQEAPAPRGQYDPLPWPDDFEVPPMPAPVEPQGPAVWEEILKNPTVKSVLNTTAREITRSIFGTGRRRRK
Prediction	85454455434522540474151645102001001334434613030216102100000010101001000100210273600000000010012003324334202400551534141330101001034763312020104201111002023044102200210031035332111104201200310142334231540340344003101300330353323200122102022203125723000000204413333200000000002200430473454721100000000020037535201510230031011230000000010540264003000010000021236502510340073047443340252047042000000012452111003202020240323414462044016403025205531335101430463356455546555545555554454455454554555645544420341023100310034001100000021343448
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEEHCCCCCCCCEEEHCHHHHHHHEEEHCCCCCCHHHHHHHHHHHHHHHCCCEEEEHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEEHCCCCCCCCCEEEEEHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHCHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEHCCCCCCCHHHHHHHCCHHHHEHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEHCCCCCCCEEEEEEEHCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MGTESAAGGPGGPAQRIAAGYTVEGQALQLGTVVVDGEPDPSAQIRIPLATVNRHGLVAGATGTGKTKTLQLIAEQLSAAGVAVLMADVKGDLSGLARPGEAADKTAARAKDTGDDWVPTAFPVEFLSLGASGVGVPVRATISSFGPILLAKVLGLNATQESTLGLIFHWADQRGLPLLDLKDLRAVITHLTSDEGKVELKSLGAVSPTTAGVILRALVNLEAEGADTFFGEPELRPEDLLRVDSQGRGIISLLEFGSQALRPAMFSTFLMWVLADLFTFLPEVGDLDKPKLVFFFDEAHLLFTDASKAFLEQVEQTVKLIRSKGVGVFFCTQLPTDLPNDVLSQLGARIQHALRAFTPDDHKALRKTVRTYPKTDVYDLESALTSLGTGEAVVTVLSEKGAPTPVAWTRMRAPRSLMAAIGAEAIGAAAQASSLQAVYGQTIDRPSAHEILSAKLAPAQEAPAQEAPAPRGQYDPLPWPDDFEVPPMPAPVEPQGPAVWEEILKNPTVKSVLNTTAREITRSIFGTGRRRRK

4d2iA

0.17

0.12

0.69

1.33

MUSTER

FLMPDIPPFAGTPDEELKSIYSQDGQ-IRIGSLI-----GKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHGEY-----------------------YDSDIKNLNRIEPK---------LNPLYMTPREFSTLLEIRENAIIQYRILRRAFIKV------TNGIRAALAAGQIPFSTLNSQFYELMADALKDEVLNKFEEFMDRYSNVI----DLTSSDIIEKVKRGK--VNVVSLTQLDEDDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKIIL--KIIEPTDKKYILESSDNLSE----DLAEQLSSLDVGEAIIIG-KIVKLPAVVKID---MFEGKLLGSDPDMIG----------------------------------------------------------------------------------------------------------

4d2iA

0.17

0.12

0.69

1.76

dPPAS

FLMPDIPPFAGTPDEELKSIYSQDGQ-IRIGSLI-----GKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHGEY-----------------------YDSDIKNLNRIEPK---------LNPLYMTPREFSTLLEIRENAIIQYRILRRAFIKV------TNGIRAALAAGQIPFSTLNSQFYELMADALKDEVLNKFEEFMDRYSNVIDLTSS----DIIEKVKRGKVNVVSLTQLDEDSMDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKIIL--KIIEPTDKKYILES----SDNLSEDLAEQLSSLDVGEAIIIGKIVKLP----AVVKIDMFEGKLLGSDPDMIG----------------------------------------------------------------------------------------------------------

4d2iA

0.17

0.12

0.69

2.73

wdPPAS

FLMPDIPPFAGTPDEELKSIYSQDGQ-IRIGSLI-----GKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHGEY-----------------------YDSDIKNLNRIEPK---------LNPLYMTPREFSTLLEIRENAIIQYRILRRAFIKV------TNGIRAALAAGQIPFSTLNSQFYELMADALKDEVLNKFEEFMDRYSNVIDLTSS----DIIEKVKRGK--VNVVSLTQLDEDDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKIIL--KIIEPTDKKYILESSDNLSE----DLAEQLSSLDVGEAIIIG-KIVKLPAVVKID---MFEGKLLGSDPDMIG----------------------------------------------------------------------------------------------------------

4d2iA

0.16

0.11

0.68

1.58

wMUSTER

FLMPDIPPFAGTPDEELKSIYSQDGQ-IRIGSLI-----GKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHGEY-----------------------YDSDIKNLNRIEPK---------LNPLYMTPREFSTLLEIRENAIIQYRILRRAFIKV------TNGIRAALAAGQIPFSTLNSQFYELMADALKDEVLNKFEEFMDRYSNVIDLTSS----DIIEKVKRGK--VNVVSLTQLDEDDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKIIL--KIIEPTDKKYILESSDNLSE----DLAEQLSSLDVGEAIIIG-KIVKLPAVVKID---MFEGKLLGSDPD-------------------------------------------------------------------------------------------------------------

4d2iA

0.17

0.12

0.69

2.95

wPPAS

FLMPDIPPFAGTPDEELKSIYSQDGQ-IRIGSLI-----GKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHGEY-----------------------YDSDIKNLNRIEPK---------LNPLYMTPREFSTLLEIRENAIIQYRILRRAFIKV------TNGIRAALAAGQIPFSTLNSQFYELMADALKDEVLNKFEEFMDRYSNVIDLTSS----DIIEKVKRGK--VNVVSLTQLDEDDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKIIL--KIIEPTDKKYILESSDNLSE----DLAEQLSSLDVGEAIIIG-KIVKLPAVVKID---MFEGKLLGSDPDMIG----------------------------------------------------------------------------------------------------------

4d2iA

0.18

0.12

0.69

5.05

dPPAS2

FLMPDIPPFAGTPDEELKSIYSQDGQ-IRIGSLI-----GKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHGEY-----------------------YDSDIKNLNRIEPK---------LNPLYMTPREFSTLLEIRENAIIQYRILRRAFIKV------TNGIRAALAAGQIPFSTLNSQFY----ELMADALKDEVLNKFEEFMDRYSNVIDLTSSDIIEKVKRGK--VNVVSLTQLDEDDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKIIL--KIIEPTDKKYILESSDNLSE----DLAEQLSSLDVGEAIIIG-KIVKLPAVVKID---MFEGKLLGSDPDMIG----------------------------------------------------------------------------------------------------------

4d2iA

0.18

0.12

0.69

2.31

PPAS

FLMPDIPPFAGTPDEELKSIYSQDGQ-IRIGSLI-----GKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHGEY-----------------------YDSDIKNLNRIEPK---------LNPLYMTPREFSTLLEIRENAIIQYRILRRAFIKV------TNGIRAALAAGQIPFSTLNSQFY----ELMADALKDEVLNKFEEFMDRYSNVIDLTSSDIIEKVKRGK--VNVVSLTQLDEDDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKIIL--KIIEPTDKKYILESSDNLSE----DLAEQLSSLDVGEAIIIG-KIVKLPAVVKI----MFEGKLLGSDPDMIG----------------------------------------------------------------------------------------------------------

4d2iA2

0.21

0.11

0.50

3.65

Env-PPAS

------------EDEELKSIYSQDGQ-IRIGSLI-----GKNVEVKLNINSFARHLAILAATGSGKSNTVAVLSQRISELGGSVLIFDYHG-----------------------EYYDSDIKNLNRIEPSD--------------------------------------------------------------------------------------------------------------IIEKVKRGKVNVVSLTQLDEDDAVVSHYLRRILDSRKDFKRSKNSGLKFPIIAVIEEAHVFLSKNENLTKYWASRIAREGRKFGVGLTIVSQRPKGLDENILSQMTNKI--ILKIIEPTDKKYILESSDNLSE----DLAEQLSSLDVGEAIIIGKIVK-LPAVVKID---MFEGKLLGSDPDMIG----------------------------------------------------------------------------------------------------------

4ag5A

0.13

0.09

0.67

1.22

MUSTER

------------------SGIN-DGSGIVLGK-------RDGGLVLVDIDRTNSNWTILAKPGAGKSFTAKMLLLREYMQGSRVIIIDPEREYKEMCRKLGGVW----------INCTGGEGKINPLQVRLRVFQSPLALHIQTLRTFFSLYLRDLTDTEKAALEDALVEVYKEAGITTDPRGVPNDKWPTVKELYEYCVKKAEE-NPETYGRLSVLLKRAAEGADSYLWAGPT-------AVEADSD--FIVFDVHDLQNAEDQVKRAQYFNVLSFAWNILERDRRE--RTVLVVDEAWMLVDPQTPQAIAFLRDTSKRIRKYNGSLIVISQNVIDFLQALLDNPTYKLLLAQ---GEKDLEAITTLMN-LSE----AEHDLLVNAKRGEGLFVAGTQR---IHIKIE-----------AAPYEMQY---------------------------------------------------------------------------------------------------------

4ag5A

0.12

0.08

0.69

1.52

dPPAS

-------------------SGINDGSGIVLGKD------RDGGLVLVDIDRTNSNWTILAKPGAGKSFTAKMLLLREYMQGSRVIIIDPEREYKEMCRKLGGVWINCTGGEGKINPLQ----------VRLRVFQSPLALHIQTLRTFFSLYLRDLTDTEKAALEDALVEVYKEGITWDTDPRGVPNDKWPTVKELYEYCVKKAEENPETYGRLSVLLKRAAEGADSYLWAGP---------TAVEADSDFIVFDVHDLQNAEDQVKRAQYFNVLSFAWNILER--DRRERTVLVVDEAWMLVDPQTPQAIAFLRDTSKRIRKYNGSLIVISQNVIDFLAPEVQRYGQALLDNYKLLLAQGEKDLEAITTLMNLSE--AEHDLLVNAKRGEGLFVAGT-----QRIHIKIEAAPYEMQY------------------------------------------------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.91

Estimated TM-score = 0.38±0.13

Estimated RMSD = 14.7±3.6Å

Download Model 2

C-score=-2.90

Download Model 3

C-score=-3.50

Download Model 4

C-score=-3.33

Download Model 5

C-score=-3.48

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4d2iA	0.667	1.72	0.163	0.692
2	4ag5A	0.525	3.89	0.141	0.621
3	1e9sF	0.523	4.54	0.127	0.642
4	3cmuA	0.408	6.09	0.081	0.565
5	2vf8B	0.402	6.28	0.084	0.574
6	3pjrA	0.399	6.77	0.068	0.593
7	2is6A	0.399	6.52	0.074	0.582
8	4c2tA	0.399	6.65	0.089	0.587
9	3pihA	0.397	6.02	0.063	0.544
10	1uaaA	0.390	6.44	0.060	0.567

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.60	28	1gkiD	ADP	Rep, Mult	61,62,63,64,65,66,67,68,414
2	0.03	2	1gkiA	ADP	Rep, Mult	54,322
3	0.02	1	4ag5A	MG	Rep, Mult	67,90

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2a4zA	0.344	7.21	0.051	0.542	2.7.1.153	NA
2	0.060	1n1hA	0.365	7.73	0.029	0.614	2.7.7.48	NA
3	0.060	2nyaA	0.350	7.06	0.045	0.540	1.7.99.4	NA
4	0.060	1uaaA	0.390	6.44	0.060	0.567	3.6.4.12	NA
5	0.060	1jqnA	0.345	7.38	0.060	0.550	4.1.1.31	349
6	0.060	2v45A	0.367	7.40	0.060	0.593	1.7.99.4	NA
7	0.060	1w36C	0.349	6.58	0.040	0.516	3.1.11.5	NA
8	0.060	2r7oA	0.361	7.43	0.056	0.589	2.7.7.48	NA
9	0.060	1w36B	0.371	6.81	0.045	0.552	3.1.11.5	83
10	0.060	1ti2A	0.370	7.24	0.038	0.585	1.97.1.2	NA
11	0.060	2qf7A	0.343	7.21	0.057	0.548	6.4.1.1	NA
12	0.060	1pjrA	0.369	7.01	0.032	0.565	3.6.4.12	NA
13	0.060	2nyaF	0.348	6.94	0.041	0.531	1.7.99.4	NA
14	0.060	1h0hA	0.369	7.57	0.054	0.612	1.2.1.2	NA
15	0.060	2vsfA	0.366	6.01	0.070	0.508	3.6.4.12	360
16	0.060	3pjrA	0.399	6.77	0.068	0.593	3.6.4.12	NA
17	0.060	1t3tA	0.345	7.39	0.046	0.557	6.3.5.3	NA
18	0.060	3crvA	0.362	5.85	0.077	0.492	3.6.4.12	63,298,300
19	0.060	2qf7B	0.344	7.21	0.063	0.548	6.4.1.1	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.30	0.667	1.72	0.16	0.69	4d2iA	GO:0000166 GO:0004386 GO:0005524 GO:0006281 GO:0006974 GO:0016787
1	0.21	0.527	4.66	0.13	0.65	1e9sA	GO:0000166 GO:0016020 GO:0016021
2	0.19	0.257	6.17	0.06	0.37	1pujA	GO:0000166 GO:0003723 GO:0003924 GO:0005525 GO:0005737 GO:0016787 GO:0042254
3	0.17	0.526	3.94	0.14	0.62	4ag6B	GO:0000166
4	0.09	0.293	7.13	0.06	0.47	2iusD	GO:0000166 GO:0000920 GO:0003677 GO:0005524 GO:0005886 GO:0005887 GO:0006970 GO:0007049 GO:0007059 GO:0009651 GO:0015616 GO:0016020 GO:0016021 GO:0016887 GO:0033676 GO:0042802 GO:0043085 GO:0043565 GO:0045893 GO:0051301 GO:0071236
5	0.06	0.389	3.85	0.15	0.45	2iutA	GO:0000166 GO:0003677 GO:0005524 GO:0005886 GO:0007049 GO:0007059 GO:0016020 GO:0016021 GO:0051301 GO:0071236
6	0.06	0.373	3.95	0.14	0.44	4ag5D	GO:0000166
7	0.06	0.335	7.28	0.04	0.54	4l22A	GO:0004645 GO:0005975 GO:0008184 GO:0016740 GO:0016757 GO:0030170
8	0.06	0.308	4.10	0.10	0.37	2w0mA	GO:0000150 GO:0000400 GO:0000730 GO:0003690 GO:0003697 GO:0004520 GO:0005524 GO:0006312 GO:0008094 GO:0010212 GO:0042148 GO:0046872
9	0.06	0.287	5.41	0.07	0.38	2qbyA	GO:0000166 GO:0003677 GO:0005524 GO:0006260
10	0.06	0.295	5.18	0.11	0.38	2pjzA	GO:0005524 GO:0016887
11	0.06	0.291	7.62	0.06	0.48	3h71B	GO:0004308 GO:0005576 GO:0005618 GO:0005737 GO:0005975 GO:0006689 GO:0008152 GO:0009313 GO:0016020 GO:0016787 GO:0016798 GO:0043231 GO:0052794 GO:0052795 GO:0052796
12	0.06	0.252	6.06	0.07	0.35	4delA	GO:0003824 GO:0004332 GO:0005576 GO:0005618 GO:0005829 GO:0005886 GO:0005975 GO:0006094 GO:0006096 GO:0008270 GO:0016829 GO:0016832 GO:0046872
13	0.06	0.268	4.21	0.13	0.32	4ag6D	GO:0000166
14	0.06	0.226	6.51	0.03	0.34	4mo2B	GO:0000166 GO:0008767 GO:0016853
15	0.06	0.255	7.23	0.04	0.41	2cmlA	GO:0004308 GO:0005975 GO:0008152 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0019012 GO:0020002 GO:0033644 GO:0046872 GO:0052794 GO:0052795 GO:0052796 GO:0055036
16	0.06	0.235	6.72	0.04	0.35	2vugA	GO:0000166
17	0.06	0.212	6.07	0.04	0.30	3h3dY	GO:0000288 GO:0000900 GO:0001709 GO:0003723 GO:0003730 GO:0005635 GO:0005737 GO:0006417 GO:0007067 GO:0007268 GO:0007275 GO:0007280 GO:0007281 GO:0007616 GO:0008258 GO:0008582 GO:0008595 GO:0016441 GO:0016477 GO:0017148 GO:0031594 GO:0042059 GO:0042078 GO:0045727 GO:0045786 GO:0045892 GO:0048149 GO:0048477 GO:0048813 GO:0050804 GO:0060213 GO:0061176 GO:0061177 GO:0097482
18	0.06	0.198	5.33	0.07	0.26	3gdhA	GO:0000387 GO:0001510 GO:0005615 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005829 GO:0006351 GO:0006355 GO:0008168 GO:0008173 GO:0009452 GO:0015030 GO:0016740 GO:0022613 GO:0030532 GO:0032259 GO:0036261 GO:0044255 GO:0071164 GO:0071167

Consensus prediction of GO terms

Molecular Function	GO:0017111	GO:0032561	GO:0003676	GO:0005524
GO-Score	0.59	0.37	0.37	0.36
Biological Processes	GO:0033554	GO:0006259	GO:0022613
GO-Score	0.59	0.59	0.37
Cellular Component	GO:0031224	GO:0044424
GO-Score	0.45	0.37

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.