I-TASSER results

I-TASSER results for job id Rv2305

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (429 residues)
MTQTLRLTALDEMFITDDIDIVPSVQIEARVSGRFDLDRLAAALRAAVAKHALARARLGR
ASLTARTLYWEVPDRADHLAVEITDEPVGEVRSRFYARAPELHRSPVFAVAVVRETVGDR
LLLNFHHAAFDGMGGLRLLLSLARAYAGEPDEVGGPPIEEARNLKGVAGSRDLFDVLIRA
RGLAKPAIDRKRTTRVAPDGGSPDGPRFVFAPLTIESDEMATAVARRPEGATVNDLAMAA
LALTILQWNRTHDVPAADSVSVNMPVNFRPTAWSTEVISNFASYLAIVLRVDEVTDLEKA
TAIVAGITGPLKQSGAAGWVVDLLEGGKVLPAMLKRQLQLLLPLVEDRFVESVCLSNLGR
VDVPAFGGEAGDTTEVWFSPTAAMSVMPIGVGLVGFGGTLRAMFRGDGRTIGGEALGRFA
ALYRDTLLT

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTQTLRLTALDEMFITDDIDIVPSVQIEARVSGRFDLDRLAAALRAAVAKHALARARLGRASLTARTLYWEVPDRADHLAVEITDEPVGEVRSRFYARAPELHRSPVFAVAVVRETVGDRLLLNFHHAAFDGMGGLRLLLSLARAYAGEPDEVGGPPIEEARNLKGVAGSRDLFDVLIRARGLAKPAIDRKRTTRVAPDGGSPDGPRFVFAPLTIESDEMATAVARRPEGATVNDLAMAALALTILQWNRTHDVPAADSVSVNMPVNFRPTAWSTEVISNFASYLAIVLRVDEVTDLEKATAIVAGITGPLKQSGAAGWVVDLLEGGKVLPAMLKRQLQLLLPLVEDRFVESVCLSNLGRVDVPAFGGEAGDTTEVWFSPTAAMSVMPIGVGLVGFGGTLRAMFRGDGRTIGGEALGRFAALYRDTLLT
Prediction	CCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEHCCCCHHHHHHHHHHHHHHHHHHCEEEEEHCCCCCEEEEEHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEHCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHCCCCCCCCCCCCCCCEEEEEEEEEHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHCCCCCCCCCCCCCCCCCCEEHCCCCCCCCCCCEEEEEEEHCCEEEEEEEHCHHHCCHHHHHHHHHHHHHHHCC
Conf.Score	987777889999999855888874789999978989999999999999999875558887478776688886777775445556666999999999869999999868999998699759999743555644549999999999998666778888886557888888758899999999876655456788877888888888766899986757899999999999899888999999999999999875888888589976669999875445566444678999978999999999999999999999867776566777655555545567788888876567775567655678887655566676545555666566678757999987998999999857669999999999999999769
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTQTLRLTALDEMFITDDIDIVPSVQIEARVSGRFDLDRLAAALRAAVAKHALARARLGRASLTARTLYWEVPDRADHLAVEITDEPVGEVRSRFYARAPELHRSPVFAVAVVRETVGDRLLLNFHHAAFDGMGGLRLLLSLARAYAGEPDEVGGPPIEEARNLKGVAGSRDLFDVLIRARGLAKPAIDRKRTTRVAPDGGSPDGPRFVFAPLTIESDEMATAVARRPEGATVNDLAMAALALTILQWNRTHDVPAADSVSVNMPVNFRPTAWSTEVISNFASYLAIVLRVDEVTDLEKATAIVAGITGPLKQSGAAGWVVDLLEGGKVLPAMLKRQLQLLLPLVEDRFVESVCLSNLGRVDVPAFGGEAGDTTEVWFSPTAAMSVMPIGVGLVGFGGTLRAMFRGDGRTIGGEALGRFAALYRDTLLT
Prediction	755442101001000101232000000000042401250023025304521300100104363642101021134142131423644123211411542031653003000201036321301100110031010200030003104433563533224213312312314303421320341242212414223222453537323223131436204301530343360101000000000000100323613233100000100004363242000000000000010463430240042015204504722311112211322411230133113301422443222210001023032332224213133131312233232210000002233110103234513454205301534562738
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEHCCCCHHHHHHHHHHHHHHHHHHCEEEEEHCCCCCEEEEEHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEHCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHCCCCCCCCCCCCCCCEEEEEEEEEHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHCCCCCCCCCCCCCCCCCCEEHCCCCCCCCCCCEEEEEEEHCCEEEEEEEHCHHHCCHHHHHHHHHHHHHHHCC
MTQTLRLTALDEMFITDDIDIVPSVQIEARVSGRFDLDRLAAALRAAVAKHALARARLGRASLTARTLYWEVPDRADHLAVEITDEPVGEVRSRFYARAPELHRSPVFAVAVVRETVGDRLLLNFHHAAFDGMGGLRLLLSLARAYAGEPDEVGGPPIEEARNLKGVAGSRDLFDVLIRARGLAKPAIDRKRTTRVAPDGGSPDGPRFVFAPLTIESDEMATAVARRPEGATVNDLAMAALALTILQWNRTHDVPAADSVSVNMPVNFRPTAWSTEVISNFASYLAIVLRVDEVTDLEKATAIVAGITGPLKQSGAAGWVVDLLEGGKVLPAMLKRQLQLLLPLVEDRFVESVCLSNLGRVDVPAFGGEAGDTTEVWFSPTAAMSVMPIGVGLVGFGGTLRAMFRGDGRTIGGEALGRFAALYRDTLLT

4zxhA1

0.12

0.11

0.97

2.41

MUSTER

VLSRHAISSEQLGIWYIQTCSAYNMVVAFDVKVNQSLNKPIEILEAVMHDYPLLRVSMPANDQGIEQLIWDRVYNIIFSDARIEASDLTQLVEQDTKQPFDLTQPPLWRIHCYECGQNYVIAFVIHHALMDFWSIGLLLRDVSKRFGLVAESDAVNG----IEFVQYADKQQSDESLIFWKNALKPHVHSIPLDYPRPAVQQHKG-SSLVFRVSESVSSGLVNLAKDY-EITLFGLVLSGFYVLLHKLSN------ENNLVIATPVAGRLERSLRNALGQFVNTIAIHMDIDADQTLRQFTQQVQEQLRQSLKHQKIAFSRVVEAVSPKRDGSINPLAQIGMFWERLGGMDEFKELLLPI--QTPATLVGQDLTLGSFPVRQQEGQLDITLEMGGEYGELVGVLKYNTDLFSAQSAENMVQLLQAVLSE

4tx2B

0.14

0.95

3.08

dPPAS

RPERVPLTARQLRAWLLAREEGRHLSVALRLRGRLDVAALEAALRDVAARHEILRTTFPGDAQTVHQHIHD-AAPVRLTPVPATEEDLPARLAERGEQLFDLTRDMPWRCELFALSEKHVLSVTVHRIAADDDSMDVFFRDLAAAYGARRAGRAPERAPLALQFADYAIWEQRLLLINDQITFWRNHLAGIDQETVRARPAIPSRRAGTVALRLDAGPHARLAEAVESAGADMPQLVQAALAMLLTRYG------AGTDLVIGTTLPDEDLIDLEPMIGPFARPFPVRTDLSADPTFLEVVARVQEAVREARQHLDVPFEKIPELLALPGSLSRHPVYQVGLQVREE--------------DGAWDAAELPALRTSVEPTGVEAIELDLAFALTERRNGIEGALHYAADLFDHDTAASLARRLVRVLEQ

5iswA2

0.17

0.16

0.96

4.07

wdPPAS

VEGEIGLTPIQHWFFEQQFTNHWNQSYMLYRPNGFDKEILLRVFNKIVEHHDALRMIYKHHNGKIVQINRGLEGTLFDLTANDNEQQVICEESARLQNSINLEVGPLVKIALFHTQNGDHLFMAIHHLVVDGISWRILFEDLATAYEQAMHQQTIALPEKTDSFKDWSIELEKYAELFLEAEYWHHLNYYTDNVQIKKDYVTMNNKRYVGMELTIEETEKLLKNVNKAYRTEINDILLTALGFALKEWAD------IDKIVINLEGHGREQMNIARTVGWFTSQYPVVLDMQKSDDLSYQIKLMKENLRRIPNKG-IGYEIFKYLTT-----------EYLRPVLPFTLKPEINFNYLGQFDSPYSMGNSLGPDGKNNLGPEGESYFVLNINGFIEEGKLHITFSYNEQQYKEDTIQQLSRSYKQHLLA

4zxhA1

0.12

0.11

0.97

3.70

wMUSTER

VLSRHAISSEQLGIWYIQTCSAYNMVVAFDVKVNQSLNKPIEILEAVMHDYPLLRVSMPANDQGIEQLIWDRVYNIIFSDARIEASDLTQLVEQDTKQPFDLTQPPLWRIHCYECGQNYVIAFVIHHALMDFWSIGLLLRDVSKRFGLVAESDAVNG----IEFVQYADKQQSDESLIFWKNALKPHVHSIPLDYPRPAVQQHKG-SSLVFRVSESVSSGLVNLAKDY-EITLFGLVLSGFYVLLHKLSN------ENNLVIATPVAGRLERSLRNALGQFVNTIAIHMDIDADQTLRQFTQQVQEQLRQSLKHQKIAFSRVVEAVSPKRDGSINPLAQIGMFWERLGGMDE--FKELLLPIQTPATLVGQDLTLGSFPVRQQEGQLDITLEMGGEYGELVGVLKYNTDLFSAQSAENMVQLLQAVLSE

4tx2B

0.15

0.14

0.95

4.64

wPPAS

RPERVPLTARQLRAWLLARTRGRHLSVALRLRGRLDVAALEAALRDVAARHEILRTTFPGDAQTVHQHIHD-AAPVRLTPVPATEEDLPARLAERGEQLFDLTRDMPWRCELFALSEKHVLSVTVHRIAADDDSMDVFFRDLAAAYGARRAGRAPERAPLALQFADYAIWEQRLLLINDQITFWRNHLAVLPFDRARPAIPSRRA-GTVALRLD-AGPHARLAEAVESAGADMPQLVQAALAMLLTRYG------AGTDLVIGTTLPDEDLIDLEPMIGPFARPFPVRTDLSADPTFLEVVARVQEAVREARQHLDVPFEKIPELLALPGSLSRHPVYQVGLQVRE--------------EDGAWDAAELPALRTSVEPTGVEAIELDLAFALTERRDGIEGALHYAADLFDHDTAASLARRLVRVLEQ

4tx2B

0.15

0.14

0.95

5.93

dPPAS2

RPERVPLTARQLRAWLLAREEGRHLSVALRLRGRLDVAALEAALRDVAARHEILRTTFPGDAQTVHQHIHD-AAPVRLTPVPATEEDLPARLAERGEQLFDLTRDMPWRCELFALSEKHVLSVTVHRIAADDDSMDVFFRDLAAAYGARRAGRAPERAPLALQFADYAIWEQRLLLINDQITFWRNHLAGIDFDRARPAIPSRRA-GTVALRLD-AGPHARLAEAVESAGADMPQLVQAALAMLLTRYG------AGTDLVIGTTLPDEDLIDLEPMIGPFARPFPVRTDLSADPTFLEVVARVQEAVREARQHLDVPFEKIPELLALPGSLSRHPVYQVGLQVREE--------------DGAWDAAELPALRTSVEPTGVEAIELDLAFALTERRNGIEGALHYAADLFDHDTAASLARRLVRVLEQ

4tx2B

0.15

0.14

0.94

3.90

PPAS

RPERVPLTARQLRAWLLARTRGRHLSVALRLRGRLDVAALEAALRDVAARHEILRTTFPGDAQTVHQHIHD-AAPVRLTPVPATEEDLPARLAERGEQLFDLTRDMPWRCELFALEKEHVLSVTVHRIAADDDSMDVFFRDLAAAYGARRAGRAPERAPLALQFADYAIWEQRLLDGEREQDSLIND-TVLPFDRARPAIPSRR--GTVALRLD-AGPHARLAEAVESAGADMPQLVQAALAMLLTRYG------AGTDLVIGTTLPDEDLIDLEPMIGPFARPFPVRTDLSADPTFLEVVARVQEAVREARQHLDVPFEKIPELLALPGSLSRHPVYQVGLQVREE--------------DGAWDAAELPALRTSVEPTGVEAIELDLAFALTERRNGIEGALHYAADLFDHDTAASLARRLVRVLEQ

4zxhA1

0.12

0.97

6.89

Env-PPAS

VLSRHAISSEQLGIWYIQRCSAYNMVVAFDVKVNQSLNKPIEILEAVMHDYPLLRVSMPANDQGIEQLIWDRVYPNFSDARHIEASDLTQLVEQDTKQPFDLTQPPLWRIHCYECQNHYVIAFVIHHALMDFWSIGLLLRDVSKRFGLVAESD----AVNGIEFVQYADKQQSDESLIFWKNALKPHVHSIPLDYPRPAVQQHKG-SSLVFRVS-ESVSSGLVNLAKDYEITLFGLVLSGFYVLLHKLSN------ENNLVIATPVAGRLERSLRNALGQFVNTIAIHMDIDADQTLRQFTQQVQEQLRQSLKHQKIAFSRVVEAVSPKRDGSINPLAQIGMFWERLGGMDEFKELLLPIQTPATL--VGQDLTLGSFPVRQQEGQLDITLEMGGEYGELVGVLKYNTDLFSAQSAENMVQLLQAVLSE

4zxjA1

0.13

0.12

0.93

2.36

MUSTER

---HLPLVAAQPGIWMAELPSAWSVAHYVELTGEVDSPLLARAVVAGLAQADTLRMRFTEDNGEVWQWVFELPEIIDLRTNIDPHGTAQALMQADLQQDLRVDSGPLVFHQLIQVADNWYWYQRYHHLLVDGFSFPAITRQIANIYCTWLRGEPTPASPFVEEYQQYRESEAWQRDAAFWAEQRRQLPPPALSPAPLPG-RSASAD-ILRLKLEFTDG-EFRQLATQLSGVQRTDLALALAALWLGRLCN------RMDYAAGFIFMRRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRDSG--RAAGDEPLFGPVLNIKVFDYQL-----------------DIPDVQAQTHTLATG-PVNDLELALFPDHGDLSIEILANKQRYDEPTLIQHAERLKMLIAQ

4zxhA1

0.12

0.11

0.97

2.94

dPPAS

VLSRHAISSEQLGIWYIQTCSAYNMVVAFDVKVNQSLNKPIEILEAVMHDYPLLRVSMPANDQGIEQLIWDRVYPNFSDARHIEASDLTQLVEQDTKQPFDLTQPPLWRIHCYECGQNYVIAFVIHHALMDFWSIGLLLRDVSKRFGLVAESDAVNG----IEFVQYADKQQSSESLIFWKNALKHAPHVHSIPLDYPRPAVQQHKGSSLVFRVSESVSSGLVNLAKDYEITLFGLVLSGFYVLLHKLSN------ENNLVIATPVAGRLERSLRNALGQFVNTIAIHMDIDADQTLRQFTQQVQEQLRQSLKHQKIAFSRVVEAVSPKRDGSINPLAQIGMFWERLGGMDEFKELLLPI--QTPATLVGQDLTLGSFPVRQQEGQLDITLEMGGEYGELVGVLKYNTDLFSAQSAENMVQLLQAVLSE

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 4 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.59

Estimated TM-score = 0.79±0.09

Estimated RMSD = 5.7±3.6Å

Download Model 2

C-score=-1.19

Download Model 3

C-score=-1.02

Download Model 4

C-score=-1.08

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4zxhA1	0.940	1.33	0.111	0.967
2	4tx2B	0.852	2.73	0.133	0.935
3	2jgpA	0.816	2.93	0.121	0.921
4	2xhgA	0.795	3.39	0.150	0.921
5	4zxjA1	0.792	3.35	0.103	0.918
6	5iswA2	0.789	3.38	0.153	0.918
7	4znmA	0.769	3.49	0.136	0.909
8	4jn3A	0.766	3.58	0.157	0.907
9	2vsqA	0.765	3.80	0.086	0.923
10	3fp0A	0.758	4.18	0.102	0.939

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.03	1	1xl7B	MPD	Rep, Mult	288,304,307,308,311
2	0.03	1	5duaA	5FQ	Rep, Mult	10,13,14,127,383
3	0.03	1	3lw5K	CLA	Rep, Mult	282,286
4	0.03	1	1s1qA	CU	Rep, Mult	127,149
5	0.03	1	1jgtB	MG	Rep, Mult	131,258
6	0.03	1	3fp0A	SWE	Rep, Mult	209,210,211,212,421
7	0.03	1	1iujA	ZN	Rep, Mult	98,127
8	0.03	1	2wscJ	CLA	Rep, Mult	106,107
9	0.03	1	3b2sA	B2S	Rep, Mult	19,22,102,127,197,284,356,389,391
10	0.03	1	3gk0B	PO4	Rep, Mult	103,126
11	0.03	1	2ii3B	CAO	Rep, Mult	208,408,412,413
12	0.03	1	1xl7B	MPD	Rep, Mult	235,236,301,302,305
13	0.03	1	1xl8B	MPD	Rep, Mult	242,246,257,260
14	0.03	1	3orcA	NUC	Rep, Mult	279,280
15	0.03	1	2xr7A	MLC	Rep, Mult	225,232,233,234,235
16	0.03	1	1f8iB	MG	Rep, Mult	77,131
17	0.03	1	3dfmA	ZN	Rep, Mult	126,131

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.095	2bghA	0.661	4.18	0.099	0.828	2.3.1.160	266,282
2	0.060	2ckjA	0.338	6.84	0.028	0.534	1.17.1.4,1.17.3.2	NA
3	0.060	3aboA	0.352	7.23	0.043	0.587	4.3.1.7	125
4	0.060	3l60A	0.379	3.48	0.105	0.436	2.3.1.12	127
5	0.060	2debA	0.567	4.85	0.071	0.748	2.3.1.21	NA
6	0.060	3b9jC	0.307	7.40	0.041	0.524	1.17.3.2,1.17.1.4	55
7	0.060	3bq5A	0.314	6.85	0.043	0.510	2.1.1.14	NA
8	0.060	2ii4A	0.379	3.47	0.099	0.438	2.3.1.168	NA
9	0.060	1bf2A	0.356	7.07	0.059	0.585	3.2.1.68	325
10	0.060	1sczA	0.375	3.52	0.099	0.438	2.3.1.61	127
11	0.060	3b9jJ	0.228	6.56	0.082	0.352	1.17.1.4,1.17.3.2	NA
12	0.060	1t7lA	0.346	7.06	0.052	0.566	2.1.1.14	NA
13	0.060	1eadA	0.382	3.54	0.141	0.443	2.3.1.12	342
14	0.060	1fo4A	0.354	7.00	0.059	0.576	1.17.1.4	NA
15	0.060	1xl7B	0.547	5.05	0.072	0.737	2.3.1.137	NA
16	0.060	1vlpB	0.352	7.12	0.071	0.578	2.4.2.11	242
17	0.060	2vr5A	0.355	7.10	0.046	0.587	3.2.1.-	NA
18	0.060	2fy3A	0.588	4.50	0.089	0.751	2.3.1.6	NA
19	0.060	2j5wA	0.358	7.01	0.050	0.585	1.16.3.1	NA
20	0.060	1q16A	0.277	7.26	0.032	0.466	1.7.99.4	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.27	0.835	2.69	0.14	0.92	4tx3B	GO:0003824 GO:0008152 GO:0009058 GO:0016788 GO:0016874 GO:0031177
1	0.26	0.783	3.41	0.16	0.91	4jn3B	GO:0003824 GO:0008152 GO:0031177
2	0.21	0.792	3.35	0.10	0.92	4zxjA	GO:0000166 GO:0003824 GO:0005524 GO:0005829 GO:0005886 GO:0008152 GO:0009058 GO:0009239 GO:0009366 GO:0016740 GO:0016779 GO:0016788 GO:0016874 GO:0031177 GO:0043041 GO:0047527
3	0.19	0.736	3.95	0.13	0.90	1l5aA	GO:0016880 GO:0019290
4	0.17	0.816	2.93	0.12	0.92	2jgpA	GO:0003824 GO:0008152 GO:0009058 GO:0016788 GO:0016874 GO:0017000 GO:0031177
5	0.14	0.765	3.80	0.09	0.92	2vsqA	GO:0003824 GO:0008152 GO:0009058 GO:0016788 GO:0016874 GO:0017000 GO:0030435 GO:0031177
6	0.06	0.320	6.71	0.06	0.51	4dg9A	GO:0000166 GO:0003824 GO:0008152
7	0.06	0.333	6.93	0.04	0.54	3kxwA	GO:0003824 GO:0008152
8	0.06	0.326	7.00	0.04	0.54	2p20A	GO:0000166 GO:0003824 GO:0003987 GO:0005524 GO:0006935 GO:0008152 GO:0016208 GO:0016874 GO:0019427 GO:0046872
9	0.06	0.321	6.78	0.05	0.52	4d56A	GO:0003824 GO:0008152
10	0.06	0.325	6.88	0.07	0.52	4dg8A	GO:0000166 GO:0003824 GO:0008152
11	0.06	0.284	7.54	0.04	0.49	4eatB	GO:0003824 GO:0005524 GO:0008152 GO:0016405 GO:0016874
12	0.06	0.328	7.59	0.06	0.58	4qflA	GO:0043190 GO:0055085
13	0.06	0.294	7.23	0.01	0.50	3a9vA	GO:0000166 GO:0003824 GO:0008152 GO:0016874
14	0.06	0.308	7.07	0.04	0.51	3e7wA	GO:0000166 GO:0003824 GO:0005524 GO:0005737 GO:0008152 GO:0016874 GO:0016876 GO:0047473 GO:0070395
15	0.06	0.303	6.84	0.07	0.49	3rg2C	GO:0000166 GO:0003824 GO:0005524 GO:0005829 GO:0008152 GO:0008668 GO:0008908 GO:0009239 GO:0016020 GO:0016740 GO:0016746 GO:0016787 GO:0016874 GO:0019290 GO:0047527
16	0.06	0.316	6.96	0.04	0.52	4r0mA	GO:0003824 GO:0008152 GO:0016740 GO:0031177
17	0.06	0.325	7.21	0.06	0.55	5es8B	GO:0003824 GO:0008152 GO:0009058 GO:0016742 GO:0016874 GO:0017000 GO:0031177
18	0.06	0.283	7.15	0.05	0.47	5ifiA	GO:0000166 GO:0003824 GO:0003987 GO:0005524 GO:0008152 GO:0016208 GO:0016874 GO:0019427

Consensus prediction of GO terms

Molecular Function	GO:0031177	GO:0016788	GO:0016881	GO:0032559	GO:0032550	GO:0016772	GO:0035639
GO-Score	0.65	0.53	0.43	0.43	0.43	0.43	0.43
Biological Processes	GO:0018130	GO:0009713	GO:0009238	GO:0019540	GO:0043038	GO:0016999
GO-Score	0.43	0.43	0.43	0.43	0.43	0.34
Cellular Component	GO:0043234	GO:0044444	GO:0071944	GO:0016020
GO-Score	0.43	0.43	0.43	0.32

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.