I-TASSER results

I-TASSER results for job id Rv2267c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (388 residues)
MKALRSSSRLSRWREWAAPLWVGCNFSAWMRLLIRNRFAVHHSRWHFAVLYTFLSMVNSC
LGLWQKIVFGRRVAETVIADPPIFIVGHWRTGTTLLHELLVVDDRHTGPTGYECLAPHHF
LLTEWFAPYVEFLVSKHRAMDNMDLSLHHPQEDEFVWCMQGLPSPYLTIAFPNRPPQYEE
YLDLEQVAPRELEIWKRTLFRFVQQVYFRRRKTVILKNPTHSFRIKVLLEVFPQAKFIHI
VRDPYVVYPSTIHLHKALYRIHGLQQPTFDGLDDKVVSTYVDLYRKLDEGRELVDPTRFY
ELRYEDLIGDPEGQLRRLYQHLGLGDFECYLPRLRQYLADHADYKTNSYQLTVEQRAIVD
EHWGEIIDRYGYDRHTPEPARLRPAVGG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MKALRSSSRLSRWREWAAPLWVGCNFSAWMRLLIRNRFAVHHSRWHFAVLYTFLSMVNSCLGLWQKIVFGRRVAETVIADPPIFIVGHWRTGTTLLHELLVVDDRHTGPTGYECLAPHHFLLTEWFAPYVEFLVSKHRAMDNMDLSLHHPQEDEFVWCMQGLPSPYLTIAFPNRPPQYEEYLDLEQVAPRELEIWKRTLFRFVQQVYFRRRKTVILKNPTHSFRIKVLLEVFPQAKFIHIVRDPYVVYPSTIHLHKALYRIHGLQQPTFDGLDDKVVSTYVDLYRKLDEGRELVDPTRFYELRYEDLIGDPEGQLRRLYQHLGLGDFECYLPRLRQYLADHADYKTNSYQLTVEQRAIVDEHWGEIIDRYGYDRHTPEPARLRPAVGG
Prediction	CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHCCCHHHHHHHHHHHHCCCCCEEEEHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
Conf.Score	9875777778756666666677867999999999855555557889999999999999999999998865554557777898899958999889999999859776566753434667777765578899999988776567655667887657999999865555665455667567777777668879999999999999999866789869986975788999999989999599998799999999999999987655677765789999999999999999999999769887798778888887999999999998999999999999999986767788876799999989999999999999998778886557876679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MKALRSSSRLSRWREWAAPLWVGCNFSAWMRLLIRNRFAVHHSRWHFAVLYTFLSMVNSCLGLWQKIVFGRRVAETVIADPPIFIVGHWRTGTTLLHELLVVDDRHTGPTGYECLAPHHFLLTEWFAPYVEFLVSKHRAMDNMDLSLHHPQEDEFVWCMQGLPSPYLTIAFPNRPPQYEEYLDLEQVAPRELEIWKRTLFRFVQQVYFRRRKTVILKNPTHSFRIKVLLEVFPQAKFIHIVRDPYVVYPSTIHLHKALYRIHGLQQPTFDGLDDKVVSTYVDLYRKLDEGRELVDPTRFYELRYEDLIGDPEGQLRRLYQHLGLGDFECYLPRLRQYLADHADYKTNSYQLTVEQRAIVDEHWGEIIDRYGYDRHTPEPARLRPAVGG
Prediction	7542374044342343024102122023003002433442324201200022013102200200421233541565613520000000000000002200210220200210100121211234213510430243232124222103202221021013002010200102220220220222441232202300410240033034564210000002003104202600140000000300100010002012212322213322232002200210130031014006614552001020220153035103300520714026601530461156357344241413362052026202500550405344365542425368
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHCCCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHCCCHHHHHHHHHHHHCCCCCEEEEHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
MKALRSSSRLSRWREWAAPLWVGCNFSAWMRLLIRNRFAVHHSRWHFAVLYTFLSMVNSCLGLWQKIVFGRRVAETVIADPPIFIVGHWRTGTTLLHELLVVDDRHTGPTGYECLAPHHFLLTEWFAPYVEFLVSKHRAMDNMDLSLHHPQEDEFVWCMQGLPSPYLTIAFPNRPPQYEEYLDLEQVAPRELEIWKRTLFRFVQQVYFRRRKTVILKNPTHSFRIKVLLEVFPQAKFIHIVRDPYVVYPSTIHLHKALYRIHGLQQPTFDGLDDKVVSTYVDLYRKLDEGRELVDPTRFYELRYEDLIGDPEGQLRRLYQHLGLGDFECYLPRLRQYLADHADYKTNSYQLTVEQRAIVDEHWGEIIDRYGYDRHTPEPARLRPAVGG

2z6vA

0.18

0.97

2.17

MUSTER

ADDVFKLAAQRTGLSEIDSDSWREGLALIVDEVNTSP--VFTPFGRQRVLDDATNALGRRLQVHAYIQDHPEVLDAPV-ERPLIVLGMPRTGTTVISYLLDQDPARRSLLHWQCVHPIPPASTETLRPRCLALLDEQRKILAKMPLPHWEDADGPTEDMFIHNQDFKGLSWDSFLPTDRYARWLFD--EADMSSTYEYQKRYLQVLQSTAPGSWSLKMPSHSVHIEALLKVFPDARLIWAHRDPYKATGSLCNLWRLPQSLVMNTLLDQTEMGRLAMWQMRYHVDRPLRARERIGDERFFHMYYHEMMRDPMDVMRRIYEWADEPLTAETEARMRNWLAHHPQDRFALNAYRLDEYGLTVEALQPIFAEY-LDTFDIELEGR------

2z6vA

0.18

0.17

0.97

2.47

dPPAS

ADDVFKLAAQRTGLSEIDSDSWREGLALIVDEVNTSPVFT--PFGRQRVLDDATNALGRRLQVHAYIQDHPEVLDAPV-ERPLIVLGMPRTGTTVISYLLDQDPARRSLLHWQCVHPIPPASTETLRTDPRCLALLDEQRKAKMPLPHWEDADGPTEDMFIHNQDFKGLSWDSFLPTDRYARWLFD--EADMSSTYEYQKRYLQVLQSTAPGSWSLKMPSHSVHIEALLKVFPDARLIWAHRDPYKATGSLCNLWRLPQSLVMNELLDQTEMGRLAMWQMRYHVDRPLRARERIGDERFFHMYYHEMMRDPMDVMRRIYEWADEPLTAETEARMRNWLAHHPQDRFALNAYRLDEYGLTVEALQPIFAEY-LDTFDIELEGR------

2z6vA

0.18

0.17

0.95

3.90

wdPPAS

ADDVFKLAAQRTGLSEIDSDSWREGLALIVDEVNTSPVFT--PFGRQRVLDDATNALGRRLQVHAYIQDHPEVLDAPV-ERPLIVLGMPRTGTTVISYLLDQDPARRSLLHWQCVHPIPPASTETLDPRCLALLDEQRKIRAKMPLPHWEDADGPTEDMFIHNQDFKGLSWDSFLPTDRYARWLFD--EADMSSTYEYQKRYLQVLQSTAPGSWSLKMPSHSVHIEALLKVFPDARLIWAHRDPYKATGSLCNLWRLPQSLVMNELLDQTEMGRLAMWQMRYHVDRPLRARERIGDERFFHMYYHEMMRDPMDVMRRIYEWADEPLTAETEARMRNWLAHHPQDRFALNAYR--TVEALQPIFAEYLDTFDIELEGR-----------

2z6vA

0.18

0.17

0.96

2.72

wMUSTER

TDDVFKLAAQRTGLSEIDSDSWREGLALIVDEVNTSPVFT--PFGRQRVLDDATNALGRRLQVHAYIQDHPEVLDAPV-ERPLIVLGMPRTGTTVISYLLDQDPARRSLLHWQCVHPIPPASTETLRPRCLALLDEQRKIRAKMPLPHWEDADGPTEDMFIHNQDFKGLSWDSFLPTDRYARWLFD--EADMSSTYEYQKRYLQVLQSTAPGSWSLKMPSHSVHIEALLKVFPDARLIWAHRDPYKATGSLCNLWRLPQSLVMNELLDQTEMGRLAMWQMRYHVDRPLRARERIGDERFFHMYYHEMMRDPMDVMRRIYEWADEPLTAETEARMRNWLAHHPQDRFALNAYRLDEYGLTVEALQPIFAEY-IELEGR-----------

2z6vA

0.18

0.17

0.95

4.72

wPPAS

ADDVFKLAAQRTGLSEIDSDSWREGLALIVDEVNTSPVFT--PFGRQRVLDDATNALGRRLQVHAYIQDHPEVLDAPV-ERPLIVLGMPRTGTTVISYLLDQDPARRSLLHWQCVHPIPPASTETLRPRCLALLDEQRKTRAKMPLPHWEDADGPTEDMFIHNQDFKGLSWDSFLPTDRYARWLFD--EADMSSTYEYQKRYLQVLQSTAPGSWSLKMPSHSVHIEALLKVFPDARLIWAHRDPYKATGSLCNLWRLPQSLVMNELLDQTEMGRLAMWQMRYHVDRPLRARERIGDERFFHMYYHEMMRDPMDVMRRIYEWADEPLTAETEARMRNWLAHHPQDRFALNAYR--TVEALQPIFAEYLDTFDIELEGR-----------

2z6vA

0.18

0.17

0.97

6.39

dPPAS2

ADDVFKLAAQRTGLSEIDSDSWREGLALIVDEVNTSPVFT--PFGRQRVLDDATNALGRRLQVHAYIQDHPEVLDAPV-ERPLIVLGMPRTGTTVISYLLDQDPARRSLLHWQCVHPIPPASTETLRTDPRCLALLDEQRKAKMPLPHWEDADGPTEDMFIHNQDFKGLSWDSFLPTDRYARWLFD--EADMSSTYEYQKRYLQVLQSTAPGSWSLKMPSHSVHIEALLKVFPDARLIWAHRDPYKATGSLCNLWRLPQSLVMNELLDQTEMGRLAMWQMRYHVDRPLRARERIGDERFFHMYYHEMMRDPMDVMRRIYEWADEPLTAETEARMRNWLAHHPQDRFALNAYRLDEYGLTVEALQPIFAEY-LDTFDIELEGR------

2z6vA

0.18

0.17

0.95

3.87

PPAS

ADDVFKLAAQRTGLSEIDSDSWREGLALIVDEVNTSPVFT--PFGRQRVLDDATNALGRRLQVHAYIQDHPEVLDAPV-ERPLIVLGMPRTGTTVISYLLDQDPARRSLLHWQCVHPIPPASTETLRPRCLALLDEQRKTRAKMPLPHWEDADGPTEDMFIHNQDFKGLSWDSFLPTDRYARWLFD--EADMSSTYEYQKRYLQVLQSTAPGSWSLKMPSHSVHIEALLKVFPDARLIWAHRDPYKATGSLCNLWRLPQSLVMNELLDQTEMGRLAMWQMRYHVDRPLRARERIGDERFFHMYYHEMMRDPMDVMRRIYEWADEPLTAETEARMRNWLAHHPQDRFALNAYR--TVEALQPIFAEYLDTFDIELEGR-----------

2z6vA

0.18

0.17

0.97

6.30

Env-PPAS

ADDVFKLAAQRTGLSEIDSDSWREGLALIVDEVNTSPVFT--PFGRQRVLDDATNALGRRLQVHAYIQDHPEVLDAPV-ERPLIVLGMPRTGTTVISYLLDQDPARRSLLHWQCVHPIPPASTETLRTDPRCLALLDEQRRAKMPLPHWEDADGPTEDMFIHNQDFKGLSWDSFLPTDRYARWLFD--EADMSSTYEYQKRYLQVLQSTAPGSWSLKMPSHSVHIEALLKVFPDARLIWAHRDPYKATGSLCNLWRLPQSLVMNELLDQTEMGRLAMWQMRYHVDRPLRARERIGDERFFHMYYHEMMRDPMDVMRRIYEWADEPLTAETEARMRNWLAHHPQDRFALNAYRLDEYGLTVEALQPIFAEY-LDTFDIELEGR------

2zq5A

0.18

0.16

0.90

2.03

MUSTER

VDELHASATKLVGLDDFGTDNYREALGVLLDAYQGEAGLT--VLGSKMNRFFLRGALVARLLSQSAWKQYPEHVDVAI-KRPIFVTGLVRTGTTALHRLLGADPAHQGLHMWLAEYPQPRPPRNPLYRQLDADFTQHHAENPGYTGLHFMAAYELEECWQLLRQSLHSVSYEALAHVPSYADWLSRQDWTPSYCRHRRNLQLIGL--NDAEKRWVLKNPSHLFALDALMATYPDALVVQTHRPVETIMASMCSLAQHTTEGWS-TKFVGAQIGADAMDTWSRGLERFNAARAKYDSAQFYDVDYHDLIADPLGTVADIYRHFGLTLSDEARQAMTT-------------SYSLADYGLTVEMVKERFAGL------------------

2zq5A

0.18

0.16

0.91

2.38

dPPAS

VDELHASATKLVGLDDFGTDDDNEALGVLLDAYQGEAGLT--VLGSKMNRFFLRGALVARLLSQSAWKQYPEHVDVAI-KRPIFVTGLVRTGTTALHRLLGADPAHQGLHMWLAEYPQPRPPRNPLYRQLDADFTQHHAENPGYTGLHFMAAYELEECWQLLRQSLHSVSYEALAHVPSYADWLSRQDWTPSYCRHRRNLQLIGL--NDAEKRWVLKNPSHLFALDALMATYPDALVVQTHRPVETIMASMCSLAQHTTEGWS-TKFVGAQIGADAMDTWSRGLERFNAARAKYDSAQFYDVDYHDLIADPLGTVADIYRHFGLTLSDEARQAMTTH------------SYSLADYGLTVEMVKERFAGL------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.04

Estimated TM-score = 0.86±0.07

Estimated RMSD = 4.6±3.0Å

Download Model 2

C-score=-1.39

Download Model 3

C-score=-1.32

Download Model 4

C-score=-4.76

Download Model 5

C-score=-0.57

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2z6vA	0.941	1.28	0.178	0.969
2	2zq5A	0.806	2.67	0.177	0.899
3	1nstA	0.483	4.39	0.111	0.606
4	4gbmA	0.481	3.80	0.168	0.575
5	3ap1A	0.475	4.38	0.158	0.598
6	4goxA	0.472	4.05	0.188	0.575
7	3cklB	0.467	4.70	0.120	0.606
8	1fmjA	0.466	4.90	0.103	0.613
9	1q20A	0.466	4.74	0.136	0.606
10	1q44A	0.465	5.11	0.105	0.629

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.32	23	4goxA	A3P	Rep, Mult	89,90,91,93,94,95,242,250,253,304,308,342,343,344
2	0.01	1	1vkjA	SO4	Rep, Mult	94,217
3	0.01	1	3ap1B	III	Rep, Mult	89,111,113,163,164,166,167,170,219,220,223,224,262,263,264,265
4	0.01	1	3ap1A	III	Rep, Mult	100,103,105,106,213
5	0.01	1	3ap1A	III	Rep, Mult	89,111,113,164,167,219,220,223,256,257,261,262,350
6	0.01	1	4eyeA	IOD	Rep, Mult	93,302,303,304
7	0.01	1	1t8uB	UUU	Rep, Mult	89,94,157,159,217,219,220,251,252,254,255,258,259,277,278
8	0.01	1	2zvqX	1NP	Rep, Mult	241,242,243,280,305,363,372
9	0.01	1	2wpnA	SBY	Rep, Mult	81,83,236,238,318,322
10	0.01	1	2ovbA	SO4	Rep, Mult	242,250,253
11	0.01	1	3ap3B	A3P	Rep, Mult	90,91,92,93,94,242,250,254,304,344,346,347,349,352
12	0.01	1	3ap2B	PO4	Rep, Mult	255,350
13	0.01	1	1j99A	HGI	Rep, Mult	319,320,323,324,325,326

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.148	3bd9A	0.461	4.11	0.128	0.572	2.8.2.23	93,242,279,308
2	0.135	1zrhA	0.462	4.07	0.126	0.572	2.8.2.23	92,242,308
3	0.060	3a8tA	0.398	4.08	0.087	0.495	2.5.1.27	92
4	0.060	2dwoA	0.400	4.70	0.079	0.533	2.7.1.105,3.1.3.46	NA
5	0.060	1aqyA	0.453	4.39	0.109	0.577	2.8.2.4	NA
6	0.060	2fhcA	0.439	6.26	0.036	0.691	3.2.1.41	218,228,279
7	0.060	1nx9A	0.404	6.57	0.053	0.649	3.1.1.43	304
8	0.060	2fhbA	0.435	6.19	0.042	0.686	3.2.1.41	221
9	0.060	1j99A	0.464	4.28	0.141	0.583	2.8.2.14,2.8.2.2	NA
10	0.060	3cklB	0.467	4.70	0.120	0.606	2.8.2.-	NA
11	0.060	3dt7A	0.421	6.69	0.019	0.691	4.1.1.32	NA
12	0.060	2wanA	0.420	6.10	0.032	0.649	3.2.1.41	223
13	0.060	2e9bA	0.414	5.76	0.050	0.616	3.2.1.41	NA
14	0.060	2zciA	0.419	6.71	0.023	0.680	4.1.1.32	NA
15	0.060	2b4kA	0.404	6.53	0.057	0.647	3.1.1.43	NA
16	0.060	1k6mA	0.401	4.48	0.093	0.526	2.7.1.105,3.1.3.46	93,220,315
17	0.060	1ii2A	0.414	6.56	0.065	0.665	4.1.1.49	NA
18	0.060	2zytX	0.464	4.59	0.150	0.590	2.8.2.9	NA
19	0.060	2vr5A	0.408	6.50	0.058	0.657	3.2.1.-	NA
20	0.060	1bglA	0.407	6.54	0.080	0.657	3.2.1.23	284
21	0.060	1q20A	0.466	4.74	0.136	0.606	2.8.2.2	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.45	0.941	1.28	0.18	0.97	2z6vA
1	0.39	0.806	2.67	0.18	0.90	2zq5A
2	0.24	0.481	3.80	0.17	0.57	4gbmA	GO:0003824 GO:0008152 GO:0016740 GO:0031177 GO:0046872
3	0.21	0.472	4.05	0.19	0.57	4goxA	GO:0003824 GO:0008152 GO:0016740 GO:0031177
4	0.14	0.408	4.09	0.15	0.50	1cjmA	GO:0004062 GO:0005737 GO:0005829 GO:0006584 GO:0006629 GO:0006805 GO:0008146 GO:0008202 GO:0009812 GO:0016740 GO:0036498 GO:0050427 GO:0051923
5	0.12	0.461	4.11	0.13	0.57	3bd9A	GO:0000139 GO:0005794 GO:0006024 GO:0006477 GO:0008146 GO:0008467 GO:0015015 GO:0016020 GO:0016021 GO:0016740 GO:0046596 GO:0050656 GO:0050819
6	0.11	0.463	4.46	0.13	0.59	1efhB	GO:0004062 GO:0004304 GO:0005737 GO:0005829 GO:0006629 GO:0007586 GO:0008146 GO:0008202 GO:0016042 GO:0016740 GO:0030573 GO:0047704 GO:0050294 GO:0050427 GO:0051923
7	0.11	0.462	4.07	0.13	0.57	1zrhA	GO:0005794 GO:0005796 GO:0006024 GO:0008146 GO:0008467 GO:0016021 GO:0016740
8	0.11	0.464	4.28	0.14	0.58	1j99A	GO:0004062 GO:0004304 GO:0005737 GO:0005829 GO:0006629 GO:0007586 GO:0008146 GO:0008202 GO:0016042 GO:0016740 GO:0030573 GO:0047704 GO:0050294 GO:0050427 GO:0051923
9	0.09	0.414	4.39	0.15	0.53	4eecA	GO:0008146 GO:0016740 GO:0017000
10	0.09	0.454	4.22	0.15	0.57	1t8tB	GO:0000139 GO:0005794 GO:0006024 GO:0008146 GO:0008467 GO:0016020 GO:0016021 GO:0016740 GO:0033872
11	0.07	0.428	4.05	0.13	0.54	1zd1A	GO:0004062 GO:0005737 GO:0005829 GO:0006629 GO:0006790 GO:0008146 GO:0008202 GO:0016740 GO:0050427
12	0.07	0.483	4.39	0.11	0.61	1nstA	GO:0000139 GO:0000165 GO:0000271 GO:0003279 GO:0003824 GO:0005794 GO:0006024 GO:0006477 GO:0006954 GO:0007224 GO:0007507 GO:0007585 GO:0008146 GO:0008152 GO:0008543 GO:0009887 GO:0015012 GO:0015016 GO:0016020 GO:0016021 GO:0016740 GO:0016787 GO:0019213 GO:0030203 GO:0030210 GO:0030900 GO:0030901 GO:0035904 GO:0048702 GO:0048703 GO:0050119 GO:0060976
13	0.07	0.475	4.21	0.16	0.59	3ap2A	GO:0000139 GO:0005783 GO:0005794 GO:0006478 GO:0008476 GO:0016020 GO:0016021 GO:0016740 GO:0070062
14	0.07	0.469	4.23	0.14	0.58	3rnlA	GO:0016740 GO:0046872
15	0.06	0.452	4.72	0.13	0.59	2gwhB	GO:0004062 GO:0005737 GO:0005829 GO:0008146 GO:0016740 GO:0050427 GO:0051923
16	0.06	0.429	4.41	0.12	0.55	2ad1A	GO:0004062 GO:0005737 GO:0005829 GO:0008146 GO:0016740 GO:0050427 GO:0051923
17	0.06	0.420	4.19	0.18	0.52	3nibA	GO:0008146
18	0.06	0.395	5.43	0.09	0.55	2jfgA	GO:0000166 GO:0005524 GO:0005737 GO:0007049 GO:0008360 GO:0008764 GO:0009058 GO:0009252 GO:0016874 GO:0042802 GO:0051301 GO:0071555

Consensus prediction of GO terms

Molecular Function	GO:0016740	GO:0043169	GO:0031177
GO-Score	0.49	0.48	0.40
Biological Processes	GO:0008152
GO-Score	0.40
Cellular Component	GO:0005829
GO-Score	0.14

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.