I-TASSER results

I-TASSER results for job id Rv2149c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (250 residues)
MLASTRHIARGDTGNVSVRIRRVTTTRAGGVSAPPFDTFNLGDHVGDDPAAVAANRARLA
AAIGLPGNRVVWMNQVHGDRVELVDQPRNTALDDTDGLVTATPRLALAVVTADCVPVLMA
DARAGIAAAVHAGRAGAQRGVVVRALEVMLSLGAQVRDISALLGPAVSGRNYEVPAAMAD
EVEAALPGSRTTTAAGTPGVDLRAGIACQLRDLGVESIDVDPRCTVADPTLFSHRRDAPT
GRFASLVWME

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MLASTRHIARGDTGNVSVRIRRVTTTRAGGVSAPPFDTFNLGDHVGDDPAAVAANRARLAAAIGLPGNRVVWMNQVHGDRVELVDQPRNTALDDTDGLVTATPRLALAVVTADCVPVLMADARAGIAAAVHAGRAGAQRGVVVRALEVMLSLGAQVRDISALLGPAVSGRNYEVPAAMADEVEAALPGSRTTTAAGTPGVDLRAGIACQLRDLGVESIDVDPRCTVADPTLFSHRRDAPTGRFASLVWME
Prediction	CCCCCCCCHCCCCCCCCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEEHCCEEEEEHCCCCCCCCCCCEEEHCCCCCEEEEEHCCCCEEEEEHCCCCEEEEEHCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCEEHCCHHHHHHHHHHHHCCCEEEHCCCCCCCCCCCCEEHCCCCCCCEEEEEEHC
Conf.Score	9988888555788888999689998799988888876677889999999999999999999989995545887765578599996787777888888998899979999868986799997999989999779678898699999999999899855489998687888765589999999999785554446799444579999999999989996997798765589765454589998558898869
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MLASTRHIARGDTGNVSVRIRRVTTTRAGGVSAPPFDTFNLGDHVGDDPAAVAANRARLAAAIGLPGNRVVWMNQVHGDRVELVDQPRNTALDDTDGLVTATPRLALAVVTADCVPVLMADARAGIAAAVHAGRAGAQRGVVVRALEVMLSLGAQVRDISALLGPAVSGRNYEVPAAMADEVEAALPGSRTTTAAGTPGVDLRAGIACQLRDLGVESIDVDPRCTVADPTLFSHRRDAPTGRFASLVWME
Prediction	7445563103143141143010000023111132212000003434133620450042016207143531120310123202202424353144000000134400000000000000000452300000001132014300320041046260514201000001012620203661154037304623433462212002320033115715052044052002125300010234520100000038
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCHCCCCCCCCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEEHCCEEEEEHCCCCCCCCCCCEEEHCCCCCEEEEEHCCCCEEEEEHCCCCEEEEEHCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEHCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCEEHCCHHHHHHHHHHHHCCCEEEHCCCCCCCCCCCCEEHCCCCCCCEEEEEEHC
MLASTRHIARGDTGNVSVRIRRVTTTRAGGVSAPPFDTFNLGDHVGDDPAAVAANRARLAAAIGLPGNRVVWMNQVHGDRVELVDQPRNTALDDTDGLVTATPRLALAVVTADCVPVLMADARAGIAAAVHAGRAGAQRGVVVRALEVMLSLGAQVRDISALLGPAVSGRNYEVPAAMADEVEAALPGSRTTTAAGTPGVDLRAGIACQLRDLGVESIDVDPRCTVADPTLFSHRRDAPTGRFASLVWME

1rw0A

0.35

0.33

0.96

3.86

MUSTER

MNA-----LIVPQWPLPKGVAACSSTRIGGVSLSPYDSLNLGAHCGDNPEHVEENRKRLFAAGNLP-SKPVWLEQVHGKNVLRLTGEPYA-SKRADASYSNTPGTVCAVMTADCLPVLFCNREGTEVAAAHAGWRGLCEGVLEETVTCF---ADKPENIIAWLGPAIGPAAFEVGPEVRDAFLAKDAQADSAFHGEKFLADIYQLARQRLANTGVEHVYGGDRCTFSESTFFSYRRDKTTGRMASFIWLI

1rw0A

0.33

0.32

0.96

6.57

dPPAS

-----MNALIVPQWPLPKGVAACSSTRIGGVSLSPYDSLNLGAHCGDNPEHVEENRKRLFAAGNLP-SKPVWLEQVHGKNVLRLTGEPYA-SKRADASYSNTPGTVCAVMTADCLPVLFCNREGTEVAAAHAGWRGLCEGVLEETVTCF---ADKPENIIAWLGPAIGPAAFEVGPEVRDAFLAKDAQADSAFHGEKFLADIYQLARQRLANTGVEHVYGGDRCTFSESEFFSYRRDKTTGRMASFIWLI

1rw0A

0.35

0.33

0.96

6.58

wdPPAS

MNA-----LIVPQWPLPKGVAACSSTRIGGVSLSPYDSLNLGAHCGDNPEHVEENRKRLFAAGNLPSK-PVWLEQVHGKNVLRLTGE-PYASKRADASYSNTPGTVCAVMTADCLPVLFCNREGTEVAAAHAGWRGLCEGVLEETVTCF---ADKPENIIAWLGPAIGPAAFEVGPEVRDAFLAKDAQADSAFHGEKFLADIYQLARQRLANTGVEHVYGGDRCTFSESEFFSYRRDKTTGRMASFIWLI

1rw0A

0.33

0.32

0.96

4.58

wMUSTER

------NALIVPQWPLPKGVAACSSTRIGGVSLSPYDSLNLGAHCGDNPEHVEENRKRLFAAGNLP-SKPVWLEQVHGKNVLRLTGEPYAS-KRADASYSNTPGTVCAVMTADCLPVLFCNREGTEVAAAHAGWRGLCEGVLEETVTCF---ADKPENIIAWLGPAIGPAAFEVGPEVRDAFLAKDAQADSAFHGEKFLADIYQLARQRLANTGVEHVYGGDRCTFSESEFFSYRRDKTTGRMASFIWLI

1rw0A

0.35

0.33

0.96

6.16

wPPAS

MNA-----LIVPQWPLPKGVAACSSTRIGGVSLSPYDSLNLGAHCGDNPEHVEENRKRLFAAGNLP-SKPVWLEQVHGKNVLRLTGE-PYASKRADASYSNTPGTVCAVMTADCLPVLFCNREGTEVAAAHAGWRGLCEGVLEETVTCF---ADKPENIIAWLGPAIGPAAFEVGPEVRDAFLAKDAQADSAFHGEKFLADIYQLARQRLANTGVEHVYGGDRCTFSESEFFSYRRDKTTGRMASFIWLI

1rw0A

0.33

0.32

0.96

11.30

dPPAS2

-----MNALIVPQWPLPKGVAACSSTRIGGVSLSPYDSLNLGAHCGDNPEHVEENRKRLFAAGNLP-SKPVWLEQVHGKNVLRLTGEPYA-SKRADASYSNTPGTVCAVMTADCLPVLFCNREGTEVAAAHAGWRGLCEGVLEETVTCF---ADKPENIIAWLGPAIGPAAFEVGPEVRDAFLAKDAQADSAFHGEKFLADIYQLARQRLANTGVEHVYGGDRCTFSESEFFSYRRDKTTGRMASFIWLI

1rw0A

0.35

0.33

0.96

5.47

PPAS

MNA-----LIVPQWPLPKGVAACSSTRIGGVSLSPYDSLNLGAHCGDNPEHVEENRKRLFAAGNLP-SKPVWLEQVHGKNVLRLTGEPYA-SKRADASYSNTPGTVCAVMTADCLPVLFCNREGTEVAAAHAGWRGLCEGVLEETVTCF---ADKPENIIAWLGPAIGPAAFEVGPEVRDAFLAKDAQADSAFHGEKFLADIYQLARQRLANTGVEHVYGGDRCTFSESTFFSYRRDKTTGRMASFIWLI

1rw0A

0.34

0.33

0.96

7.08

Env-PPAS

MNA-----LIVPQWPLPKGVAACSSTRIGGVSLSPYDSLNLGAHCGDNPEHVEENRKRLFAAGNLP-SKPVWLEQVHGKNVLRLTGEPYA-SKRADASYSNTPGTVCAVMTADCLPVLFCNREGTEVAAAHAGWRGLCEGVLEETVTCF---ADKPENIIAWLGPAIGPAAFEVGPEVRDAFLAKDAQADSAFHGEKFLADIYQLARQRLANTGVEHVYGGDRCTFSESEFFSYRRDKTTGRMASFIWLI

1rv9A

0.36

0.34

0.95

3.78

MUSTER

------KNFLTADWPAPANVKTLITTRNGGVSQGAYQSLNLGTHVGDNPEAVRRNREIVQQQVGLP---VAYLNQIHSTVVVNAAEALGGT-PDADASVDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMK---VPPVEMMAYLGPAISADAFEVGQDVFDAFCTPMPEAATAFGSGKFLADLYALARLILKREGVGGVYGGTHCTVLERTFFSYRRDGATGRMASLIWLD

1rv9A

0.35

0.34

0.95

6.37

dPPAS

------KNFLTADWPAPANVKTLITTRNGGVSQGAYQSLNLGTHVGDNPEAVRRNREIVQQQVGLP---VAYLNQIHSTVVVNAAEALGG-TPDADASVDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMK---VPPVEMMAYLGPAISADAFEVGQDVFDAFCTPMPEAATAIGSGKFLADLYALARLILKREGVGGVYGGTHCTVLERDFFSYRRDGATGRMASLIWLD

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 1 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.83

Estimated TM-score = 0.97±0.05

Estimated RMSD = 2.2±1.7Å

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1rw0A	0.933	1.11	0.346	0.960
2	1t8hA	0.913	2.19	0.324	0.996
3	1rv9A	0.907	1.40	0.342	0.948
4	1xfjA	0.890	2.03	0.331	0.968
5	1t3tA2	0.496	5.03	0.085	0.736
6	5ah1A	0.489	5.77	0.054	0.816
7	2hs3A	0.474	5.19	0.068	0.708
8	2z5gA	0.460	5.89	0.061	0.780
9	4efdA	0.458	6.02	0.066	0.792
10	1h2xA	0.457	5.36	0.042	0.720

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.20	2	1u05A	ZN	Rep, Mult	77,114,131
2	0.17	3	1wb4A	SXX	Rep, Mult	77,78,79,135,136,140
3	0.17	2	1t8hA	ZN	Rep, Mult	170,171,224,227
4	0.06	1	1gklA	FER	Rep, Mult	135,137,138,139,182
5	0.06	1	1oi8A	CO3	Rep, Mult	166,225,226

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	3b9eA	0.443	5.78	0.043	0.740	3.2.1.14	NA
2	0.060	2hs4A	0.449	5.06	0.053	0.668	6.3.5.3	164
3	0.060	2ddtA	0.412	6.08	0.081	0.720	3.1.4.12	NA
4	0.060	1t3tA	0.496	5.03	0.085	0.736	6.3.5.3	133
5	0.060	1w0iA	0.430	5.46	0.075	0.680	2.3.1.41	NA
6	0.060	1tz7A	0.449	5.49	0.031	0.712	2.4.1.25	NA
7	0.060	1e5tA	0.457	5.39	0.058	0.720	3.4.21.26	234
8	0.060	1ho5A	0.439	5.39	0.053	0.676	3.1.3.5,3.6.1.45	NA
9	0.060	1c7jA	0.310	6.56	0.039	0.596	3.1.1.-	NA
10	0.060	1thgA	0.445	5.82	0.051	0.764	3.1.1.3	112
11	0.060	1maaD	0.432	6.04	0.071	0.732	3.1.1.7	NA
12	0.060	1w9pA	0.432	5.85	0.073	0.720	3.2.1.14	NA
13	0.060	2a3eA	0.431	5.86	0.078	0.720	3.2.1.14	74,226
14	0.060	2w22A	0.456	5.90	0.060	0.776	3.1.1.3	206
15	0.060	1eveA	0.432	5.94	0.034	0.740	3.1.1.7	NA
16	0.060	1qo9A	0.432	6.01	0.063	0.752	3.1.1.7	NA
17	0.060	2pffD	0.452	5.58	0.060	0.708	2.3.1.86	NA
18	0.060	1edqA	0.439	5.67	0.082	0.720	3.2.1.14	NA
19	0.060	1mx9D	0.435	6.04	0.054	0.752	3.1.1.1	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.66	0.928	1.24	0.36	0.96	1u05A	GO:0046872
1	0.33	0.913	2.19	0.32	1.00	1t8hA	GO:0046872
2	0.07	0.447	5.24	0.08	0.68	3viuA	GO:0000166 GO:0000287 GO:0004642 GO:0005524 GO:0005737 GO:0006164 GO:0006189 GO:0016874 GO:0046872
3	0.07	0.426	5.22	0.07	0.63	2hruA	GO:0000166 GO:0000287 GO:0004642 GO:0005524 GO:0005737 GO:0006164 GO:0006189 GO:0006541 GO:0009156 GO:0016874 GO:0046872
4	0.06	0.428	2.87	0.18	0.50	2f9zC	GO:0006935 GO:0008984 GO:0016787 GO:0050568
5	0.06	0.409	5.64	0.07	0.65	1r9jB	GO:0003824 GO:0004802 GO:0008152 GO:0016740 GO:0046872
6	0.06	0.375	4.78	0.08	0.56	4q1tD	GO:0000166 GO:0003723 GO:0004349 GO:0005524 GO:0005737 GO:0006561 GO:0008652 GO:0016301 GO:0016310 GO:0016740 GO:0055129
7	0.06	0.401	4.97	0.06	0.60	1e3wD	GO:0001540 GO:0003857 GO:0004303 GO:0005496 GO:0005739 GO:0007569 GO:0008033 GO:0016491 GO:0018454 GO:0030283 GO:0030331 GO:0033327 GO:0042802 GO:0047015 GO:0051287 GO:0051289 GO:0055114
8	0.06	0.474	5.19	0.07	0.71	2hs3A	GO:0000166 GO:0000287 GO:0004642 GO:0005524 GO:0005737 GO:0006164 GO:0006189 GO:0006541 GO:0009156 GO:0016874 GO:0046872
9	0.06	0.378	6.30	0.04	0.67	1jy1A	GO:0000012 GO:0003690 GO:0003697 GO:0004518 GO:0004527 GO:0005634 GO:0005737 GO:0005886 GO:0006281 GO:0006302 GO:0006974 GO:0008081 GO:0016787 GO:0017005 GO:0043231 GO:0090305
10	0.06	0.350	6.33	0.03	0.63	1a12A	GO:0000082 GO:0000790 GO:0000794 GO:0003677 GO:0003682 GO:0005085 GO:0005087 GO:0005634 GO:0005654 GO:0005737 GO:0007049 GO:0007052 GO:0007059 GO:0007067 GO:0007088 GO:0016032 GO:0031492 GO:0031965 GO:0042393 GO:0043547 GO:0051225 GO:0051301
11	0.06	0.355	6.25	0.05	0.62	4ufoA	GO:0003824 GO:0016787
12	0.06	0.417	5.26	0.08	0.64	3vdqA	GO:0000166 GO:0003858 GO:0016491 GO:0055114
13	0.06	0.321	5.76	0.06	0.54	1pmiA	GO:0004476 GO:0005737 GO:0005975 GO:0008270 GO:0009298 GO:0016853 GO:0031505 GO:0046872
14	0.06	0.345	5.95	0.05	0.58	4zhtA	GO:0000166 GO:0003824 GO:0004553 GO:0005524 GO:0005737 GO:0005829 GO:0006045 GO:0006047 GO:0006054 GO:0007155 GO:0008152 GO:0008761 GO:0009384 GO:0016301 GO:0016310 GO:0016740 GO:0016787 GO:0046835 GO:0046872
15	0.06	0.358	6.09	0.04	0.64	3ikmD	GO:0002020 GO:0003676 GO:0003677 GO:0003682 GO:0003887 GO:0005739 GO:0005760 GO:0006259 GO:0006260 GO:0006261 GO:0006264 GO:0006287 GO:0007568 GO:0008408 GO:0009416 GO:0010332 GO:0016740 GO:0016779 GO:0042645 GO:0043195 GO:0043234 GO:0055093 GO:0070062 GO:0071333 GO:0071897 GO:0090305
16	0.06	0.332	5.94	0.06	0.56	1a5zA	GO:0003824 GO:0004459 GO:0005737 GO:0005975 GO:0006096 GO:0016491 GO:0016616 GO:0019752 GO:0055114
17	0.06	0.330	6.29	0.04	0.60	1vr0C	GO:0000287 GO:0016311 GO:0016787 GO:0019295 GO:0050532
18	0.06	0.295	5.06	0.09	0.45	1ztcA

Consensus prediction of GO terms

Molecular Function	GO:0046872
GO-Score	0.80
Biological Processes	GO:0006189	GO:0006935	GO:0006541
GO-Score	0.13	0.07	0.07
Cellular Component	GO:0005737
GO-Score	0.13

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.