I-TASSER results

I-TASSER results for job id Rv2091c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (244 residues)
MSGPQGSDPRQPWQPPGQGADHSSDPTVAAGYPWQQQPTQEATWQAPAYTPQYQQPADPA
YPQQYPQPTPGYAQPEQFGAQPTQLGVPGQYGQYQQPGQYGQPGQYGQPGQYAPPGQYPG
QYGPYGQSGQGSKRSVAVIGGVIAVMAVLFIGAVLILGFWAPGFFVTTKLDVIKAQAGVQ
QVLTDETTGYGAKNVKDVKCNNGSDPTVKKGATFECTVSIDGTSKRVTVTFQDNKGTYEV
GRPQ

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MSGPQGSDPRQPWQPPGQGADHSSDPTVAAGYPWQQQPTQEATWQAPAYTPQYQQPADPAYPQQYPQPTPGYAQPEQFGAQPTQLGVPGQYGQYQQPGQYGQPGQYGQPGQYAPPGQYPGQYGPYGQSGQGSKRSVAVIGGVIAVMAVLFIGAVLILGFWAPGFFVTTKLDVIKAQAGVQQVLTDETTGYGAKNVKDVKCNNGSDPTVKKGATFECTVSIDGTSKRVTVTFQDNKGTYEVGRPQ
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCCEEEEEEEHCCCCCEEHCCCC
Conf.Score	9999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999999998888877766443454544445566677777777776889888888864788877777766667899988755578657899996897568999998599856757999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MSGPQGSDPRQPWQPPGQGADHSSDPTVAAGYPWQQQPTQEATWQAPAYTPQYQQPADPAYPQQYPQPTPGYAQPEQFGAQPTQLGVPGQYGQYQQPGQYGQPGQYGQPGQYAPPGQYPGQYGPYGQSGQGSKRSVAVIGGVIAVMAVLFIGAVLILGFWAPGFFVTTKLDVIKAQAGVQQVLTDETTGYGAKNVKDVKCNNGSDPTVKKGATFECTVSIDGTSKRVTVTFQDNKGTYEVGRPQ
Prediction	7642546726434344444463444244444434444244444432233434433344344344334341323424334332323333453323323333332433444433323332444332333445444444433433331112232222213332322223354144631552044114443432326405505137565350565350404042656645040303166251425548
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCCEEEEEEEHCCCCCEEHCCCC
MSGPQGSDPRQPWQPPGQGADHSSDPTVAAGYPWQQQPTQEATWQAPAYTPQYQQPADPAYPQQYPQPTPGYAQPEQFGAQPTQLGVPGQYGQYQQPGQYGQPGQYGQPGQYAPPGQYPGQYGPYGQSGQGSKRSVAVIGGVIAVMAVLFIGAVLILGFWAPGFFVTTKLDVIKAQAGVQQVLTDETTGYGAKNVKDVKCNNGSDPTVKKGATFECTVSIDGTSKRVTVTFQDNKGTYEVGRPQ

2pffB

0.14

0.96

1.47

MUSTER

GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-------GGGGGGGGGGGGGGGG--GGGGGG

1mv3A

0.09

0.07

0.80

1.34

dPPAS

----DGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATQLKAGVIPFQNPEEQESDWNQHKKLEKCRGVFPENFTERVP---------------------------------------------

1mv3A

0.13

0.09

0.75

0.98

wdPPAS

----DGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQA-----------------QHD------------------YTATDTDEL--------------QLK-----AG--DVVLVIPFQDEGNF-TERP-

2pffB

0.14

0.94

1.63

wMUSTER

VAGTLDINPDDDYGFKQGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG-------GGGGGG-----GGGGGG---GGGG

1mv3A

0.12

0.09

0.75

1.40

wPPAS

-DGSPAATPEIR--VNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQ------------------HD------------------YTATDTDEL--------------QLK-----AG--DVVLVPFQNDEGNF-TERP-

1mv3A

0.10

0.07

0.76

2.14

dPPAS2

----DGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATQLKAGVLVIPFQNPEEESDWNQHKKLEKCRG-------------------------------------------------------

1mv3A

0.09

0.07

0.82

1.87

PPAS

-DGSPAATPEIR--VNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKV--------------------------------QAQHD----YTATDTDELQLKAGDVVLVIPFQ--NPEEQDEG---WLMGVKESDWNQHKKLEKC

2miiA

0.13

0.10

0.77

0.84

Env-PPAS

------------------------------------GSHMVGQREPAPVEEVKPAPEQPAEPQQPVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSVLLV----------DSVNNRTNGSLNAAEATETLRNALANNGKFT--LVSAQQLSMAKQQLGLSPQDSLGTRS-------KAIGIARNVGAHYVLYSSASGNVNTLQMQLMLVQGKGAVSQQ-

1zlgA

0.09

1.00

1.12

MUSTER

QTRLKSAKVSLHFTSTHATNNKEQLVKTRKGGIQTQLPFQRRRPTRPLEVGAPFKTEDPTFPEACAHNRTTGSEASSGMTHENYIILQDSFSCKYKVTVQPIRPKSHSKAEAVFFTTPPCSALKGKSHKPIGCLGEAGHVLSKVLAKPENLSASFIVQDVNITGHFSWKMAKANLYQPMTGFQVTWAEVTTESRQNSLPNSIISQSQILPSDHYVLTVP-PSTLYRLEVQVLTPGGTFRTSSAH

5gaoE

0.08

1.00

1.27

dPPAS

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.98

Estimated TM-score = 0.29±0.09

Estimated RMSD = 15.5±3.3Å

Download Model 2

C-score=-4.09

Download Model 3

C-score=-4.34

Download Model 4

C-score=-3.63

Download Model 5

C-score=-4.67

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2pffB	0.724	3.48	0.126	0.897
2	2uvcG	0.723	3.56	0.039	0.906
3	3hmjG	0.718	3.64	0.056	0.910
4	4z9rA	0.605	3.21	0.032	0.750
5	4z38A	0.583	3.21	0.039	0.730
6	4q4kA	0.583	3.35	0.062	0.725
7	3tsbA	0.582	3.73	0.075	0.754
8	1me7A	0.576	3.78	0.072	0.754
9	4fxsA1	0.576	3.65	0.094	0.742
10	4fezA	0.575	3.79	0.088	0.750

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.11	6	3usbA	IMP	Rep, Mult	62,167,168,169,170,197,198,199,241,242
2	0.06	3	3nm1B	IFP	Rep, Mult	58,60,82,113,143,159,162,195,197
3	0.06	3	3q4dI	ALA	Rep, Mult	82,84,113,115,143,164,241
4	0.04	2	2y7dA	ZN	Rep, Mult	60,62,242
5	0.02	1	3oahA	CYT	Rep, Mult	163,164,165
6	0.02	1	3uufA	MAN	Rep, Mult	184,185
7	0.02	1	3mi1B	FE	Rep, Mult	187,189
8	0.02	1	3cv1A	CA	Rep, Mult	99,102
9	0.02	1	2h3cA	NUC	Rep, Mult	226,228,230
10	0.02	1	1c9uB	CA	Rep, Mult	82,91
11	0.02	1	3fbyA	CA	Rep, Mult	10,12,14,16,17,18,21
12	0.02	1	3dyoB	IPT	Rep, Mult	93,95
13	0.02	1	4c6cA	ZN	Rep, Mult	59,197,233
14	0.02	1	4e0sB	MAN	Rep, Mult	43,44,77
15	0.02	1	1cklF	UUU	Rep, Mult	234,237
16	0.02	1	3pvbB	ANP	Rep, Mult	134,137

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.066	1xi3A	0.513	3.79	0.060	0.676	2.5.1.3	NA
2	0.066	1xi3B	0.508	3.55	0.066	0.660	2.5.1.3	143
3	0.060	1zfjA	0.571	3.56	0.064	0.734	1.1.1.205	NA
4	0.060	1nf7A	0.573	3.68	0.047	0.746	1.1.1.205	239
5	0.060	2bmbA	0.569	5.14	0.053	0.881	2.5.1.15,2.7.6.3,4.1.2.25	NA
6	0.060	1f76A	0.550	3.88	0.068	0.738	1.3.3.1	71,116
7	0.060	1rboB	0.556	4.47	0.076	0.783	4.1.1.39	NA
8	0.060	2uv8G	0.721	3.73	0.056	0.918	2.3.1.86	NA
9	0.060	1bxnA	0.533	4.99	0.065	0.807	4.1.1.39	NA
10	0.060	3cuxA	0.550	4.12	0.037	0.754	2.3.3.9	NA
11	0.060	2pffB	0.724	3.48	0.126	0.897	2.3.1.86	116,123,140,158
12	0.060	1gthA	0.557	3.79	0.045	0.734	1.3.1.2	NA
13	0.060	1vcgB	0.554	3.76	0.057	0.725	5.3.3.2	NA
14	0.060	2zryD	0.559	3.91	0.053	0.754	5.3.3.2	115
15	0.060	1iwaA	0.553	4.36	0.056	0.775	4.1.1.39	NA
16	0.060	2c6qG	0.551	3.74	0.086	0.730	1.7.1.7	65,116
17	0.060	2i9uA	0.548	4.67	0.028	0.779	3.5.4.3	NA
18	0.060	2d69A	0.549	4.47	0.075	0.770	4.1.1.39	NA
19	0.060	1bxnE	0.533	5.02	0.055	0.807	4.1.1.39	116
20	0.060	2rdtA	0.515	3.99	0.049	0.697	1.1.3.15	NA
21	0.060	2a1yA	0.550	3.36	0.041	0.701	1.7.1.7	63,116

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.07	0.477	4.80	0.04	0.70	5c7hA	GO:0000166 GO:0016491 GO:0055114
1	0.06	0.451	3.77	0.04	0.60	3ceuA	GO:0003824 GO:0004789 GO:0005737 GO:0009228
2	0.06	0.316	6.18	0.03	0.57	4bmsF	GO:0000166
3	0.06	0.364	5.73	0.07	0.61	3anlA	GO:0008299 GO:0016114 GO:0016491 GO:0019288 GO:0030145 GO:0030604 GO:0042802 GO:0046872 GO:0051484 GO:0055114 GO:0070402
4	0.06	0.323	6.23	0.03	0.58	4bmnA	GO:0000166
5	0.06	0.307	6.66	0.05	0.59	2qo3B	GO:0003824 GO:0008152 GO:0016491 GO:0016740 GO:0016746 GO:0017000 GO:0031177 GO:0033068 GO:0047879 GO:0048037 GO:0055114
6	0.06	0.354	5.54	0.05	0.59	3omlA	GO:0003824 GO:0004300 GO:0005777 GO:0006629 GO:0006631 GO:0006635 GO:0008152 GO:0016491 GO:0016616 GO:0016829 GO:0033540 GO:0042803 GO:0055114 GO:0080023
7	0.06	0.252	6.54	0.03	0.48	1tzsA	GO:0004190 GO:0005768 GO:0006508 GO:0007586 GO:0008233 GO:0016540 GO:0016787 GO:0019886 GO:0030163 GO:0042803 GO:0070062
8	0.06	0.300	6.17	0.02	0.53	3g87A	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746
9	0.06	0.355	6.49	0.03	0.67	5f9tA	GO:0004308 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0052794 GO:0052795 GO:0052796
10	0.06	0.318	6.31	0.04	0.58	3hjvA	GO:0003824 GO:0004314 GO:0006633 GO:0008152 GO:0016740 GO:0016746
11	0.06	0.357	5.90	0.02	0.60	2ghiA	GO:0000166 GO:0005524 GO:0006810 GO:0016020 GO:0016021 GO:0016887 GO:0042626 GO:0055085
12	0.06	0.314	6.91	0.05	0.62	3tzwA	GO:0003824 GO:0005618 GO:0005829 GO:0005886 GO:0008152 GO:0009058 GO:0016740 GO:0016747 GO:0016788 GO:0019367 GO:0031177 GO:0034081 GO:0040007 GO:0051260 GO:0071768 GO:0071769
13	0.06	0.338	6.05	0.06	0.59	2aj4A	GO:0000166 GO:0000411 GO:0004335 GO:0005524 GO:0005737 GO:0005975 GO:0006012 GO:0008152 GO:0016301 GO:0016310 GO:0016740 GO:0016773 GO:0033499 GO:0046835
14	0.06	0.298	5.68	0.06	0.50	2h1yB	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746
15	0.06	0.325	6.22	0.02	0.58	3rgiA	GO:0003824 GO:0004314 GO:0008152 GO:0016740
16	0.06	0.299	6.78	0.05	0.58	3h0pB	GO:0003824 GO:0004314 GO:0006629 GO:0006631 GO:0006633 GO:0008152 GO:0016740 GO:0016746
17	0.06	0.309	6.63	0.04	0.59	4rl1A	GO:0003824 GO:0008152 GO:0016740 GO:0031177
18	0.06	0.326	6.11	0.05	0.58	3qatA	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746

Consensus prediction of GO terms

Molecular Function	GO:1901265	GO:0036094
GO-Score	0.36	0.36
Biological Processes	GO:0055114	GO:0009228	GO:0051484
GO-Score	0.12	0.07	0.06
Cellular Component	GO:0005737
GO-Score	0.07

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.