I-TASSER results

I-TASSER results for job id Rv2079

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (656 residues)
MQLRHINIRALIAEAGGDPWAIEHSLHAGRPAQIAELAEAFHAAGRYTAEANAAFEEARR
RFEASWNRENGEHPINDSAEVQRVTAALGVQSLQLPKIGVDLENIAADLAEAQRAAAGRI
ATLESQLQRIDDQLDQALELEHDPRLAAAERSELDALITCLEQDAIDDTASALGQLQSIR
AGYSDHLQQSLAMLRADGYDGAGLQGLDAPQSPVKPEEPIQIPPPGTGAPEVHRWWTSLT
SEERQRLIAEHPEQIGNLNGVPVSARSDANIAVMTRDLNRVRDIATRYRTSVDDVLGDPA
KYGLSAGDITRYRNADETKKGLDHNARNDPRNPSPVYLFAYDPMAFGGKGRAAIAIGNPD
TAKHTAVIVPGTSSSVKGGWLHDNHDDALNLFNQAKAADPNNPTAVIAWMGYDAPNDFTD
PRIATPMLARIGGAALAEDVNGLWVTHLGVGQNVTVLGHSYGSTTVADAFALGGMHANDA
VLLGCPGTDLAHSAASFHLDGGRVYVGAASTDPISMLGQLDSLSQYVNRGNLAGQLQGLA
VGLGTDPAGDGFGSVRFRAEVPNSDGINPHDHSYYYHRGSEALRSMADIASGHGDALASD
GMLAQPRHQPGVEIDIPGLGSVEIDIPGTPASIDPEWSRPPGSITDDHVFDAPLHR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MQLRHINIRALIAEAGGDPWAIEHSLHAGRPAQIAELAEAFHAAGRYTAEANAAFEEARRRFEASWNRENGEHPINDSAEVQRVTAALGVQSLQLPKIGVDLENIAADLAEAQRAAAGRIATLESQLQRIDDQLDQALELEHDPRLAAAERSELDALITCLEQDAIDDTASALGQLQSIRAGYSDHLQQSLAMLRADGYDGAGLQGLDAPQSPVKPEEPIQIPPPGTGAPEVHRWWTSLTSEERQRLIAEHPEQIGNLNGVPVSARSDANIAVMTRDLNRVRDIATRYRTSVDDVLGDPAKYGLSAGDITRYRNADETKKGLDHNARNDPRNPSPVYLFAYDPMAFGGKGRAAIAIGNPDTAKHTAVIVPGTSSSVKGGWLHDNHDDALNLFNQAKAADPNNPTAVIAWMGYDAPNDFTDPRIATPMLARIGGAALAEDVNGLWVTHLGVGQNVTVLGHSYGSTTVADAFALGGMHANDAVLLGCPGTDLAHSAASFHLDGGRVYVGAASTDPISMLGQLDSLSQYVNRGNLAGQLQGLAVGLGTDPAGDGFGSVRFRAEVPNSDGINPHDHSYYYHRGSEALRSMADIASGHGDALASDGMLAQPRHQPGVEIDIPGLGSVEIDIPGTPASIDPEWSRPPGSITDDHVFDAPLHR
Prediction	CCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHCCCCCCCCEEEEEHCCCCCCCCEEEHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHCHHHHHHHHHHHHCCCCCCEEEEHCCCCCCCCCCHHHHCCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	98767767888877578875678999879999999999999999998999999999999988887645654567889999999999999999999999999999999999999999999999999998877766555554567787777657788999999999999999999999999999999999999997655667766555566777776666666788888999999999969999999999988688767899999999999999999999999988888877777665677765555799999999999999999987655788988489998578888885799997688888858999599999988776788679999999999987889986899997899999988865579789999999999999999986578988589999866799999999976998766999958998888898885988885898658999766677778888888887777777777887778888888886786578898888887777667899988999999988578876788887777766666777888888877778888755577778887777888888887789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MQLRHINIRALIAEAGGDPWAIEHSLHAGRPAQIAELAEAFHAAGRYTAEANAAFEEARRRFEASWNRENGEHPINDSAEVQRVTAALGVQSLQLPKIGVDLENIAADLAEAQRAAAGRIATLESQLQRIDDQLDQALELEHDPRLAAAERSELDALITCLEQDAIDDTASALGQLQSIRAGYSDHLQQSLAMLRADGYDGAGLQGLDAPQSPVKPEEPIQIPPPGTGAPEVHRWWTSLTSEERQRLIAEHPEQIGNLNGVPVSARSDANIAVMTRDLNRVRDIATRYRTSVDDVLGDPAKYGLSAGDITRYRNADETKKGLDHNARNDPRNPSPVYLFAYDPMAFGGKGRAAIAIGNPDTAKHTAVIVPGTSSSVKGGWLHDNHDDALNLFNQAKAADPNNPTAVIAWMGYDAPNDFTDPRIATPMLARIGGAALAEDVNGLWVTHLGVGQNVTVLGHSYGSTTVADAFALGGMHANDAVLLGCPGTDLAHSAASFHLDGGRVYVGAASTDPISMLGQLDSLSQYVNRGNLAGQLQGLAVGLGTDPAGDGFGSVRFRAEVPNSDGINPHDHSYYYHRGSEALRSMADIASGHGDALASDGMLAQPRHQPGVEIDIPGLGSVEIDIPGTPASIDPEWSRPPGSITDDHVFDAPLHR
Prediction	52044040220034131131322430452425204400500340042254345104403530453254442431342243034225314201420440043034004302401430343043133304403430330131243442224334303420441244025203400530440054015203401430443424324132143443423443242343443424302400440356104300540042013131011420230024305531541454244244224323233333332243142044045315304423543564432000000013444330100001010330410000000000204322233222103202420453455320000000000002102323011221034005303400400211253540100000000000000100333614211000000000012320440415411000030331003201312211321232321230221301103012142120120213222442221110010023624002000000013244034642334422333041521112425131242202011415325532366241525448
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHCCCCCCCCEEEEEHCCCCCCCCEEEHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHCHHHHHHHHHHHHCCCCCCEEEEHCCCCCCCCCCHHHHCCCCCEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCCC
MQLRHINIRALIAEAGGDPWAIEHSLHAGRPAQIAELAEAFHAAGRYTAEANAAFEEARRRFEASWNRENGEHPINDSAEVQRVTAALGVQSLQLPKIGVDLENIAADLAEAQRAAAGRIATLESQLQRIDDQLDQALELEHDPRLAAAERSELDALITCLEQDAIDDTASALGQLQSIRAGYSDHLQQSLAMLRADGYDGAGLQGLDAPQSPVKPEEPIQIPPPGTGAPEVHRWWTSLTSEERQRLIAEHPEQIGNLNGVPVSARSDANIAVMTRDLNRVRDIATRYRTSVDDVLGDPAKYGLSAGDITRYRNADETKKGLDHNARNDPRNPSPVYLFAYDPMAFGGKGRAAIAIGNPDTAKHTAVIVPGTSSSVKGGWLHDNHDDALNLFNQAKAADPNNPTAVIAWMGYDAPNDFTDPRIATPMLARIGGAALAEDVNGLWVTHLGVGQNVTVLGHSYGSTTVADAFALGGMHANDAVLLGCPGTDLAHSAASFHLDGGRVYVGAASTDPISMLGQLDSLSQYVNRGNLAGQLQGLAVGLGTDPAGDGFGSVRFRAEVPNSDGINPHDHSYYYHRGSEALRSMADIASGHGDALASDGMLAQPRHQPGVEIDIPGLGSVEIDIPGTPASIDPEWSRPPGSITDDHVFDAPLHR

4ut1A

0.10

0.07

0.77

1.03

PPAS

----------------------SNQLNA-QGSSLSTYYTLVAQLNNYVGSPTAGIATAITNYFTGLQ----TVANADPSARQTAMSNAQTLASQLVAAGQQYSQLRQSVNSQLTDTVTQINSYTSQIAQLNEQIASASS----------------------PNQLLDQRDLAVSKLSQL-------------QVVQSNGNYSVFLSGGQPLVVGNASYQLATVASPSDPSELTIVSKGVAGSAQPGPTQYLPDVS--LTGGALGGLLAFRSQTLDPAQAQLGALAVSFASQVNAQNALGVDMSGNP----------------------GGSLFAVGAPAVYANQNNTGSATLSVSFVDGTTTSDYALSYDGAKYTLTDRATGSVVGTATP--------SSTPPTMTIGGLKLSLSSTPNAGDSFTVLPTRGALDGFSLATANGSAIAAASPVLAAGVATNSGTGVISQAGYQLPSGTTTLAYNAASKGFPVGTTVTIAGTPPTSINITSATTPVPYDPSKGASMTISSTTQPAPSGVMNGVSVSLSGTPADGDQFTIGANKGTNDGRNALANSKTMNNGTAAQTALVGQITQAQQSVS----------------------------------------------------------

3eb7A

0.10

0.08

0.82

1.26

Env-PPAS

-----ISERDAVKTAIS---LVGTILGKLGVPLVGPIVSLYSTLIDVLPGGKSQWEIFMEQVEALINQKI------AEYARAKALAELEGLGNNYQLYLTALEEWQEN-----PSSTRVLRDVRNRFEILDSLFTQYMPSFRVTGYEVP----------------------LLSVYAQAANLHLLLLKDASIFGEEWGF----------------------------STTAINNYYNRQMAQYSDHCVQWYRTGLDRLKGSNAKQWVEYNRFRREMTLSVLDIMTLFPMYDMRTYPMETDPIGAIGAQGSWYDSA-PSFNTLESTFIRGKHLFDTRLSIYTGRSSFSASNYLKKWIGH----QISSQPIGGSIQTQTYGTTSG-----------------SSVIATQQIG--FTGFDVYKTLSTAGVLFAYTSKYY-----GVSKVVF----DAIYPDNKYKTTFTYNPGSEGIGAQEKDSEVELPPETLDQPNYEIRNPDVPVFSWTRSADRTNTVYSDASDGPKPSANEVGHYLGGDPISF---NSSGSTGVIRLNINSPLSQKYRCSSVDFDLD----VVRGGTTVNNGRFNKSAPNVGWQSLKYENFKFASFS----TPFTFNQAQDTLKISVRNFSSIVGGSVVY---IDR

4b2nA

0.11

0.09

0.87

1.01

PPAS

LDQASIRSPLMVGCNGKDPRSLVKQGVNSNPNALHNPPPPFVNRLPPRPTTCGQFRASATRGRVNLEERQFFQPMALATSYHFIFLQWGYLIRPPDFEEQVSKRYGLYPAPFRNPYPLPGEDPNQTNGGSGQGKDDNGRWTGKWGLPNSANDAGLLASDMFRTTPITALGNLLPLPWSTGRGSSDAIGLISLLPALFDMETLTLAPSLLEYVADAPHAGMTKAPA---------WWAR--AFKTRQ---------GSLSSDNVHSEMAFGVANIANARRGLEDEFEDINNFLISLSPATYPKTINTALAEQGAVIYHERDLWASGANGAIPKPAGNG--VYSPRHAADPNYLPDPRLKGVAAVVTPIETIRTDP-----------RRMRLMADERQRRAWNSG-----WWAYNNLSPSWTGYPSDNIVASELRRVPRAIYNNGGPIYSPLGPNIWEEPTGYIAPPLYGAWATAPYFHN-----GSVP-----NLWGVLKPSDRPKLWKRPYTAAGIGGKNAGY---------------DYSFASYDWQKLGWKYTAVACSIFTSPFLPCTHNMATIDILYSMWDNVAAQYLNLAYQSPPPITDQQIKSRMVYNSYLYGND------------------------NGGHDFTQSL-R

2c9kA

0.11

0.09

0.86

1.24

Env-PPAS

LSAYTIVVGTVLTGFGGLALIGFGTLIPVAQDQSNTWSDFITQTKNIIKKASTYISNANKILNRSFNVISTYHNHLKTWENNPNPQNTQDVRTQIQLVHYHFQNVIPELVNSCPPNPSDCDYYNILVLSSYAQAANLHLTVL--------NQAVKFEAYLKNNTAIDYYPVLTKAIEDYTNYCVTTYKKGLNLIKT---------------------TPDSNLDGNINWNTYNTYRTKM-TTAVLDLVALFPNYDVGK--YPIGVQSELTREIYQVLNFEESPYKYYDFQYQEDSLTRRPHLFTWLDSLNFYE------------KAQTTPNNFFTSHYNMFHYTLDNISQKSSVFGNHNVTDKLKSLGLATNIYIFLLNV---------ISLDNKYLNDYNNISKMDFFITN---GTRLLEKELTAGSGQITYDVNKNIFGLPIKRREETLFPTYDNYSH--------------ILSFIKSLSIPATYKTQ---------VYTFAWTSSVDPKNTIYTHLTTQIPAVKANSLGTASKVVQGPG-------HGGDLIDFKDHFKITCQHSNFQQYASNGS---ANTRAVINLSIPGVAELGMALNPTFSGTDYTNLK-YKDFQYLEFSNEVKFAPNQNNRSDVYTNTTVL---IDK

1ciyA

0.12

0.10

0.83

1.23

Env-PPAS

IDISLSLTQFLLSEFVPGAGFVLGLVDIIWGIFGPSQWDAFLVQIEQLRIEEFARNQAISRLEGLSNLY--QIYAESFREWEANPALREEMRIQFNDMNSALTTAIPLLAYQVPLLSVYVQAANLHLSVLRDVSVFGQRWGFDAATINSRYNDLTRLIGNYTDYAVRWYNTGLERVWGPDSRDWVRYNQFRRELTLTVLDIVALFSNYDSRRYPIRTVSQLTREIYTNPVLENFDGSFRGMAQRIEQNIRQPHLMDILNSITIYTDVHRGFNYWSGHQITASPVGFSGPEFAFPLFGNAGNAAPPVLVSLTGLGIFRTLSSPLYRRIILGSGPNNQELFVLD-------GTEFSFASLTTNLPSTIYRQRGTVDSLDVIPPQDNSVPPRAMLSQAAGAVYTLRAPTFSWQHRSAFNNIIPSSQITQIPLTKS-----------TNLGSGT----SVVGFTGG----------------DILRRTSPG-QISTLRVNITAPLSQRYRVRSTTNLQFHT--------SIDGRPINQGNFSATMSSGSNLQSGSFRTVGFTTPFNFSNGSSVFTLSAHVFNSGNEVYRIEFVPAEVT--------------------------------------------------------------

1yvlB

0.11

0.09

0.87

1.20

Env-PPAS

MEIRQY-LAQWLEKQ---DWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGG---------------------PPNACLDQLQNWFTIVSLQQVRQQLKKLEELEQTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRKTGVQFTVKLRLQELNYNLRNTVKGFRKFNILGTHTKVMNMEESTNGSRHLQLKEQGPLIVTEETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWAS--------ILWYNVAEPRNLSFFLTPPCAR-------WAQLSEVLSWQFS----SVTKRGLNDQLNMLGEKLLGPNASPDGLIPWNINDKNFPESILELIKKHLLLWN----DGCI-----MGFISKERERALLKDQQPGTFLLRFSE--SSREGAITFTWVERSNGGEPDFHAVEPYTKKELSAVTFPDIIRNY----------------KVMAAENIPENPLKYLYPNIDHAFGKYYSR-----------------

1ji6A

0.09

0.07

0.82

1.16

Env-PPAS

---------DAVGTGIS---VVGQILGVVGVPFAGALTSFYQSFLNTIPSDADPWKAFMAQVEVLIDKKEEYAKSKALAELQGLQNNFEDYVNALNSWKKTPLSLRS--KRSQDRIRELFSQAESHFRNSMPSFA-----------VSKFEVLFLPTYAQAANTHLLLLKDAQVFGEEWG----------------------------------------------YSSEDVAEFYHRQLQQYTDHCVNWYNVGLNGLRGSTYDAWVKFNRFRREMTLTVLDLIVLFPFYDIRLYSKGVKTELTRTDPIFSLNTLQEYGPTFLSIENSIRKPHLFDYLQGIEFPGYFGKDSFNYWSGN----YVETRPSIGSSKTITSPFYGDKSTEPVQKLS-----FDGQKIANTDVAAWPNGKVYLGVTKVD--------------FSQYDDQKNETSTQTYDSKRNNGHVSAQDSIDQ------------LPPETTDEPLEKAYSHQLNYAECFLMQDRRGTIPFFTWTHRSVDFFNTIDAEKAYALSSGEGPGFTGGNLLFLKESSNSIAKFKVTLNSAYASTTNLRL-----FVQNSNNDFLVIYINKTMNKDDDLTYQATTNSNMGFSGDKNELIIGAESFVSNEKI----YID----K

4hnwA

0.12

0.11

0.94

0.97

MUSTER

NGSTNKQIYRDLATLIGDFKNALVSRKKYYRANWTSLAVAQDVNGE-RQQAINTLSQFEKLAEGKISDSEKEHSECLMYKNDIMYKAASDNQDKLQNVLKHLNDIEPCVFD-KFGLLERKATIYMKLGQLKDASIVYRTLIKRN---PDNFKYYKLLEVSLG--IQGDNKLKKALYGKLEQFYPRCEPPKFIPLTFLQDKEELSKKLREYVLPQLERGV------PATFSNVKPLYQRRKSKVSPLLEKIVLDYLSGLDPTQDPIPFIWTNYYLSQHFLFLKDFPKAQEYIDAALDHTPTLVEFYILKARILKHLGLMDTAAGILEEGRQLDLQDRFIN-------CKTVKYFLRANNIDKAVEVASLFTKNDDSVNGIKDLHLVEASWFIVEQAEAYYRLYLDRKKKLDDLASEQIANDIKENQWLVRKYKGLALKRFNAIPKFYKQFEDDQL----HSYCMRKGTPRAYLEMLEWGKALY-------TKPMYVRAMKEASKLY-FQMHDDRLKRSQKKKAKKEAAAMNKRKETEAKSVAAYPSDQDNDVFGEKLIETSTPMEVREDERDYI-NYRIGKASLNKFAKRFGTTSGLFGSMAIVLLHATRNDTPFDPILKKVVTKSLEKNDINGLLFLYR----YRDDVPIGSSNLK

4uxvA

0.10

0.08

0.79

0.70

dPPAS

--------------------KIYAEIDRLESWKIEILNRSIVEHLKMTGQTEEFFEKWREEWDEIVTAHMPKVEELYDAEENADKYRFKKANQVLVHIDDLLTAAESSIEKILREISDLVTSEEKSREEIEQVRERYSKSRKNLLAYSHLYGELYDSLEKDLDEIWYITARKVLLEQDRNLERLQSYIDDVPKLLADCKQTVPGQIAKLKDGYGEMKEKGYKLEHIQLDKELENLSNQLKRAEHVLMTELD------------IDEASAILQLIDENIQSVYQQLEGEVEAGQSVLSKMP-------------ELIIAYDKLKEEKEHTKAE-----------TELVKESYR-----------------------LTAGELGKQQAFEKRLDEIGKLLSSVKDKL----------DAEHVAYSLLVEEVASIEKQIEEVKKEHAEYRENLQA----------------LRKEELQARETLSNLKKTISETARLLKTSNIPGIPSHIQEMLENAHHHIQETVNQLNELPLNMEEAGAHLKQAEDIVNRASRESEELVEQVILIEKIIQFGNRFRSQNHILSEQL------KEAERRFYAFDYDDSYEIAAAAVEKAAPGAVEKIKAD------------------------------

5ic0A

0.10

0.06

0.61

0.76

wdPPAS

------------HMAPGD--TVRELLENPVFGCLDSVMENSKVLGEAMTGISQNAKNGLPEFGDAIAT--SKALCGFTEAAAQAAYLVGVSPTQFARANQAIQMACQSLGETQAQVLSAATIVAKHTSALCNSCRLASARTAN--------PTAKRQFVQSAKEVANSTANLVKTIKALDGDFTEENRAQCRAATAPLLEAVDNLSAFASNPEFSSVPAQISPEGRAAMEPIVISAKTMDPPRWSVLAGHSRTVSDSIKKLITSMRDKA-QLECETAIAALNSCLRDLDQASLAAVSQQLAPREGISQEALHTQMLTAVQEISHLIEPLASAARA---------EASQLGHKVSQMA--QYFEPLTLAAVGAASKTLSHPQQMAAESALQLLYTAKEAGGN-------------PKQAAHTQEALEEAVQMMTEAVEDLTTTLNEAAS----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.81

Estimated TM-score = 0.39±0.13

Estimated RMSD = 15.0±3.5Å

Download Model 2

C-score=-3.06

Download Model 3

C-score=-3.51

Download Model 4

C-score=-4.09

Download Model 5

C-score=-4.52

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3eb7A	0.766	2.83	0.105	0.826
2	1dlcA	0.744	3.11	0.099	0.816
3	1ji6A	0.741	3.23	0.091	0.819
4	1ciyA	0.718	3.22	0.097	0.793
5	2c9kA	0.712	3.84	0.065	0.809
6	4w8jA	0.705	3.30	0.097	0.782
7	1i5pA	0.638	4.74	0.082	0.773
8	1w99A	0.636	4.06	0.071	0.741
9	4d8mA	0.580	4.70	0.079	0.701
10	3x0uA	0.488	3.96	0.081	0.567

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.06	4	2ckjD	FES	Rep, Mult	574,575,577,579,580,581,582,584,590
2	0.06	4	1fo4A	FES	Rep, Mult	303,304,484,485,486,497,499
3	0.05	3	1ea0A	F3S	Rep, Mult	446,447,448,450,451,452,453,464
4	0.03	2	1dmrA	O	Rep, Mult	412,460
5	0.03	2	5d92A	8K6	Rep, Mult	81,84,88
6	0.02	1	3ovnA	MPV	Rep, Mult	462,466
7	0.02	1	3np5A	CA	Rep, Mult	185,188
8	0.02	1	2qtsC	MAL	Rep, Mult	89,93
9	0.02	1	1hjsA	EPE	Rep, Mult	409,415,422
10	0.02	1	3or3A	CA	Rep, Mult	592,593
11	0.02	1	2bf0X	CA	Rep, Mult	134,137
12	0.02	1	1utzA	PF3	Rep, Mult	572,573
13	0.02	1	3iovA	CA	Rep, Mult	97,101
14	0.02	1	2hl6A	CXS	Rep, Mult	323,372,459,460,572,591,592
15	0.02	1	1oczB	HEA	Rep, Mult	98,130
16	0.02	1	1y10C	CA	Rep, Mult	411,413
17	0.02	1	2zo4A	ZN	Rep, Mult	459,476,572,595
18	0.02	1	3eubS	FES	Rep, Mult	424,425,442,443,444,445,447
19	0.02	1	4fhvA	MG	Rep, Mult	462,463,479
20	0.02	1	1jwlA	NUC	Rep, Mult	265,269

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2gbcA	0.311	7.85	0.040	0.494	3.4.14.5,3.4.15.5	NA
2	0.060	1ofdA	0.355	8.15	0.045	0.582	1.4.7.1	NA
3	0.060	3cmmC	0.320	8.13	0.052	0.523	6.3.2.19	249
4	0.060	1fa9A	0.328	7.78	0.049	0.523	2.4.1.1	NA
5	0.060	2pdaA	0.324	7.88	0.039	0.518	1.2.7.1	353,423
6	0.060	1n1hA	0.324	8.27	0.056	0.530	2.7.7.48	536
7	0.060	1llwA	0.354	8.27	0.055	0.588	1.4.7.1	NA
8	0.060	2d5lA	0.316	7.83	0.043	0.503	3.4.14.-	NA
9	0.060	1ahpA	0.328	8.13	0.044	0.537	2.4.1.1	NA
10	0.060	1vlbA	0.305	8.64	0.023	0.520	1.2.99.7	251
11	0.060	3b9jC	0.243	8.69	0.034	0.416	1.17.3.2,1.17.1.4	NA
12	0.060	1dgjA	0.316	8.57	0.055	0.538	1.2.-.-	266
13	0.060	1bxrA	0.336	8.41	0.061	0.572	6.3.5.5	59
14	0.060	1orvA	0.334	8.00	0.037	0.540	3.4.14.5	NA
15	0.060	1e1yA	0.319	8.16	0.029	0.520	2.4.1.1	NA
16	0.060	3b9jJ	0.194	6.15	0.071	0.265	1.17.1.4,1.17.3.2	259,269
17	0.060	2qllA	0.314	8.17	0.038	0.514	2.4.1.1	NA
18	0.060	2cqsA	0.339	7.99	0.044	0.546	2.4.1.20	NA
19	0.060	3ffzA	0.373	8.13	0.052	0.607	3.4.24.69	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.21	0.766	2.83	0.10	0.83	3eb7A	GO:0005102 GO:0006952 GO:0009405 GO:0030435
1	0.20	0.741	3.23	0.09	0.82	1ji6A	GO:0005102 GO:0006952 GO:0009405 GO:0030435
2	0.13	0.744	3.11	0.10	0.82	1dlcA	GO:0005102 GO:0006952 GO:0009405 GO:0030435
3	0.12	0.718	3.22	0.10	0.79	1ciyA	GO:0005102 GO:0006952 GO:0009405 GO:0030435
4	0.11	0.705	3.30	0.10	0.78	4w8jA	GO:0005102 GO:0006952 GO:0009405 GO:0030435
5	0.11	0.712	3.84	0.07	0.81	2c9kA	GO:0005102 GO:0006952 GO:0009405 GO:0030435
6	0.10	0.688	4.23	0.07	0.81	4moaA	GO:0005102 GO:0006952 GO:0009405 GO:0030435
7	0.07	0.638	4.74	0.08	0.77	1i5pA	GO:0005102 GO:0006952 GO:0009405 GO:0030435
8	0.07	0.580	4.70	0.08	0.70	4d8mA	GO:0009405 GO:0030435
9	0.07	0.488	3.96	0.08	0.57	3x0uA	GO:0009405
10	0.06	0.213	6.31	0.06	0.30	4bvkB	GO:0016787
11	0.06	0.206	7.30	0.03	0.32	3odpA	GO:0005975 GO:0016853 GO:0030246
12	0.06	0.174	6.18	0.05	0.24	3o38A	GO:0004316 GO:0016491 GO:0055114 GO:0102132
13	0.06	0.178	6.47	0.05	0.25	4p4oA	GO:0003677 GO:0003887 GO:0005634 GO:0006281 GO:0034061 GO:0071897
14	0.06	0.201	6.56	0.02	0.29	1g6oA	GO:0000166 GO:0005524 GO:0005737 GO:0006810 GO:0009405
15	0.06	0.159	6.63	0.04	0.23	4k3vB	GO:0006810 GO:0007155 GO:0030001 GO:0046872
16	0.06	0.161	6.13	0.03	0.22	1nlzC	GO:0000166 GO:0005524 GO:0005737 GO:0006810 GO:0009405
17	0.06	0.143	3.72	0.03	0.16	1yo7A	GO:0006351 GO:0006355
18	0.06	0.136	5.79	0.04	0.18	2hhiA	GO:0005576 GO:0005618 GO:0009267

Consensus prediction of GO terms

Molecular Function	GO:0005102
GO-Score	0.57
Biological Processes	GO:0009405	GO:0030435	GO:0006952
GO-Score	0.57	0.57	0.57
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.