I-TASSER results

I-TASSER results for job id Rv2042c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (265 residues)
MAPPNRDELLAAVERSPQAAAAHDRAGWVGLFTGDARVEDPVGSQPQVGHEAIGRFYDTF
IGPRDITFHRDLDIVSGTVVLRDLELEVAMDSAVTVFIPAFLRYDLRPVTGEWQIAALRA
YWELPAMMLQFLRTGSGATRPALQLSRALLGNQGLGGTAGFLTGFRRAGRRHKKLVETFL
NAASRADKSAAYHALSRTATMTLGEDELLDIVELFEQLRGASWTKVTGAGSTVAVSLASD
HRRGIMFADVPWRGNRINRIRYFPA

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MAPPNRDELLAAVERSPQAAAAHDRAGWVGLFTGDARVEDPVGSQPQVGHEAIGRFYDTFIGPRDITFHRDLDIVSGTVVLRDLELEVAMDSAVTVFIPAFLRYDLRPVTGEWQIAALRAYWELPAMMLQFLRTGSGATRPALQLSRALLGNQGLGGTAGFLTGFRRAGRRHKKLVETFLNAASRADKSAAYHALSRTATMTLGEDELLDIVELFEQLRGASWTKVTGAGSTVAVSLASDHRRGIMFADVPWRGNRINRIRYFPA
Prediction	CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHCCEEEHCCEEEEEEEEEEEHCCCEEEEHCCEEEEEEEEHCCCCEEEEEEHCCCHHHHHHHHHHCCCCCCCCHHHCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEHCCCCCCCHHHHHHHHHCCCCCEEEEHCCEEEEEEHCCCCCEEEEEEHCCCCCEEEEEEEHCC
Conf.Score	9999999999999999999998999999998469968878999998786899999999975887699997676874988999999999988978998765899999998897658888643697999999985688766655554578887657467888988864778567889999999987665688999868887588689987799999999987987668875886999998589876999997788885789998769
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MAPPNRDELLAAVERSPQAAAAHDRAGWVGLFTGDARVEDPVGSQPQVGHEAIGRFYDTFIGPRDITFHRDLDIVSGTVVLRDLELEVAMDSAVTVFIPAFLRYDLRPVTGEWQIAALRAYWELPAMMLQFLRTGSGATRPALQLSRALLGNQGLGGTAGFLTGFRRAGRRHKKLVETFLNAASRADKSAAYHALSRTATMTLGEDELLDIVELFEQLRGASWTKVTGAGSTVAVSLASDHRRGIMFADVPWRGNRINRIRYFPA
Prediction	8555437403500420151047322610020005602000013473340250035004422345404142644221332001202020314442323240212010131246210310202142650143036444442320241243104323231021012002312550352034003213434333034213451304147743142440153047241320001232000003355320000000244343033033238
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEHCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEHCCEEEHCCEEEEEEEEEEEHCCCEEEEHCCEEEEEEEEHCCCCEEEEEEHCCCHHHHHHHHHHCCCCCCCCHHHCCHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEHCCCCCCCHHHHHHHHHCCCCCEEEEHCCEEEEEEHCCCCCEEEEEEHCCCCCEEEEEEEHCC
MAPPNRDELLAAVERSPQAAAAHDRAGWVGLFTGDARVEDPVGSQPQVGHEAIGRFYDTFIGPRDITFHRDLDIVSGTVVLRDLELEVAMDSAVTVFIPAFLRYDLRPVTGEWQIAALRAYWELPAMMLQFLRTGSGATRPALQLSRALLGNQGLGGTAGFLTGFRRAGRRHKKLVETFLNAASRADKSAAYHALSRTATMTLGEDELLDIVELFEQLRGASWTKVTGAGSTVAVSLASDHRRGIMFADVPWRGNRINRIRYFPA

3rgaA

0.16

0.15

0.96

1.47

MUSTER

M--PAETVRKEVALEYCRRVNAGELEGVLQLFAPDARLVDPLGTEPVVGRAALAARLAPALRGA-VHEEPGRPYAAGTSVVLPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDS--SWTARPAPDEERRKELAREHCLRINDGDVDGLLKLYDPVGSTGLEALRAHATMAVGSNGLTVAGQDGRHAAVTVSATMDYLPSGPLLARHHLMTLPAPADPHRALIGIE-----YVMVIGVDADG-LIDEMRAYAT

3rgaA

0.16

0.15

0.95

1.80

dPPAS

-HMPAETVRKEVALEYCRRVNAGELEGVLQLFAPDARLVDPLGTEPVVGRAALAARLAPALRGA-VHEEPGRPYAAGTSVVLPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDS------SWTARPAPDEERRKELAREHCLRINDGDVDGLLSWTRTGLEALRAHATMAVGSNVLTVAGQDGRHAAVTVSATMDYLPSGPLLARHHLMTLPAPADPHRALIGIE-----YVMVIGVDADGL-IDEMRAYWG

3rgaA

0.19

0.16

0.85

2.31

wdPPAS

MP--AETVRKEVALEYCRRVNAGELEGVLQLFAPDARLVDPLGTEPVVGRAALAARLAPALRAVHEEPGRPYAAHDGTSVVLPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDS--------SWTARPA---------------------------ERRKELAREHCLRINDGDVDGLLKLYSPRIRFEDPVGSW-ALRAHATMAVGSNVRVAGQDGRHAAVTVSAHRAEYVMVIGVDADG-LIDEMRAYAT

3rgaA

0.19

0.17

0.86

1.63

wMUSTER

M--PAETVRKEVALEYCRRVNAGELEGVLQLFAPDARLVDPLGTEPVVGRAALAARLAPALRGA-VHEEPGRPYAAGTSVVLPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDS--------SWTARPA------------------------PDEERRKELAREHCLRINDGDVDGLLKLYSPRIRFEVGS-WTRALRAHATMAVGSNVGLTVAGGRHAAVTVSAHRALYVMVIGVDADG-LIDEMRAYAT

3rgaA

0.19

0.16

0.85

2.20

wPPAS

M--PAETVRKEVALEYCRRVNAGELEGVLQLFAPDARLVDPLGTEPVVGRAALAARLAPALRAVHEEPGRPYAAHDGTSVVLPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDSSWT-----------------------------------ARPAERRKELAREHCLRINDGDVDGLLKLYSPRIRFEVGSW-TRTLRAHATMAVGSNVRLTVAGGRHAAVTVSAHRALYVMVIGVDADG-LIDEMRAYAT

3rgaA

0.16

0.15

0.94

1.99

PPAS

M--PAETVRKEVALEYCRRVNAGELEGVLQLFAPDARLVDPLGTEPVVGRAALAARLAPALRGA-VHEEPGRPYAAGTSVVLPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDS------SWTARPAPDEERRKELAREHCLRINDGDVDGLL-VGSTGLEALRAHATMAVGSNVLTVAGQDGRHAAVTVSATMDYLPSGPLLARHHLMTLPAPADPHRALIGIE-----YVMVIGVDADG-LIDEMRAYAT

1iskA

0.26

0.12

0.47

2.87

dPPAS2

M--NTPEHMTAVVQRYVAALNAGDLDGIVALFADDATVEDPVGSEPRSGTAAIREFYANSLKLPLAVELTQEVRAVANEAAFAFTVSFEYQGRKTVVAP----IDHFRFNGAGKVVSMRALFGEKNIHAGA--------------------------------------------------------------------------------------------------------------------------------------

3rgaA

0.16

0.15

0.97

2.62

Env-PPAS

M--PAETVRKEVALEYCRRVNAGELEGVLQLFAPDARLVDPLGTEPVVGRAALAARLAPALRGA-VHEEPGRPYAAGTSVVLPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDSSWTARPAPDEERRKELAREHCLRINDGVDGLLKLYSPVGSWTRTGLEALRAHATMAVGSNVLTVAGQDGRHAAVTVSATMDYLPSGPLLARHHLMTLPAPADPHRALIGIE-----YVMVIGVDADG-LIDEMRAYAT

1iskA

0.27

0.13

0.47

1.02

MUSTER

M--NTPEHMTAVVQRYVAALNAGDLDGIVALFADDATVEDPVGSEPRSGTAAIREFYANSLKLPLAVELTQEVRAVANEAAFAFTVSFEYQGRKTVVAP----IDHFRFNGAGKVVSMRALFGEKNI-----HAGA---------------------------------------------------------------------------------------------------------------------------------

1iskA

0.26

0.12

0.47

1.66

dPPAS

--MNTPEHMTAVVQRYVAALNAGDLDGIVALFADDATVEDPVGSEPRSGTAAIREFYANSLKLPLAVELTQEVRAVANEAAFAFTVSFEYQGRKTVVAP----IDHFRFNGAGKVVSMRALFGEKNIHAGA--------------------------------------------------------------------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.21

Estimated TM-score = 0.56±0.15

Estimated RMSD = 8.6±4.5Å

Download Model 2

C-score=-2.31

Download Model 3

C-score=-3.38

Download Model 4

C-score=-2.46

Download Model 5

C-score=-4.33

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3rgaA	0.870	2.16	0.157	0.962
2	3i4zA	0.478	5.55	0.060	0.766
3	3o24A	0.477	5.23	0.067	0.725
4	4ld7A	0.473	5.25	0.070	0.713
5	5injA	0.455	5.55	0.077	0.717
6	4yl7A	0.454	5.18	0.069	0.694
7	4e0tA2	0.445	5.45	0.079	0.694
8	5eanA	0.442	5.31	0.054	0.687
9	1e3vB	0.440	1.84	0.213	0.472
10	2xepA	0.438	5.18	0.044	0.653

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.52	55	1ogxA	EQU	Rep, Mult	16,40,84,86,88,101,105,118,122
2	0.03	3	2a15A	NCA	Rep, Mult	16,28,40,66,86,105,107,118
3	0.02	2	1oh00	III	Rep, Mult	6,7,10,42,43,72,73,74,75,76,77,79,81,83,84,102,103,104,106,121,122,123,124,125,126
4	0.02	3	3junB	AJD	Rep, Mult	28,53,56,57,60,64,67,120

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	3d4jB	0.406	6.08	0.063	0.691	4.1.1.33	NA
2	0.060	3psgA	0.384	6.30	0.053	0.672	3.4.23.1	16
3	0.060	4stdA	0.430	2.59	0.091	0.491	4.2.1.94	69
4	0.060	2bngC	0.414	2.12	0.152	0.457	3.3.2.8	121
5	0.060	1lrwA	0.434	5.71	0.031	0.664	1.1.99.8	NA
6	0.060	3i4zA	0.478	5.55	0.060	0.766	2.5.1.34	NA
7	0.060	1gpeB	0.359	6.24	0.080	0.630	1.1.3.4	194
8	0.060	1w6sC	0.431	5.71	0.039	0.657	1.1.99.8	71
9	0.060	1fi4A	0.418	6.20	0.058	0.706	4.1.1.33	10
10	0.060	1hn0A	0.393	6.29	0.056	0.675	4.2.2.20	23
11	0.060	2psgA	0.390	6.13	0.049	0.657	3.4.23.1	21,110
12	0.060	3lrfA	0.393	5.54	0.073	0.615	2.3.1.41	12,75
13	0.060	1kitA	0.413	5.53	0.028	0.641	3.2.1.18	NA
14	0.060	2d0vA	0.433	5.72	0.039	0.664	1.1.99.8	NA
15	0.060	1e3vB	0.440	1.84	0.213	0.472	5.3.3.1	40,57,105
16	0.060	1gpeA	0.406	6.06	0.054	0.691	1.1.3.4	NA
17	0.060	2w68C	0.247	6.01	0.041	0.411	3.2.1.18	NA
18	0.060	1flhA	0.364	6.20	0.077	0.626	3.4.23.1	NA
19	0.060	1lrwC	0.433	5.72	0.031	0.664	1.1.99.8	67

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.37	0.421	2.41	0.22	0.47	1buqA	GO:0004769 GO:0005622 GO:0006629 GO:0006810 GO:0008202 GO:0016853
1	0.30	0.875	2.14	0.16	0.97	4rzmB	GO:0016853 GO:0017000
2	0.21	0.442	2.04	0.17	0.48	3wmdB	GO:0016853
3	0.16	0.438	1.88	0.18	0.47	5cxoB	GO:0016787
4	0.12	0.439	1.94	0.20	0.47	3t8nB	GO:0004769 GO:0006629 GO:0008202 GO:0016853
5	0.07	0.473	5.25	0.07	0.71	4ld7A	GO:0009820 GO:0050364
6	0.07	0.478	5.55	0.06	0.77	3i4zA	GO:0009820 GO:0016740 GO:0035835 GO:0050364
7	0.07	0.477	5.23	0.07	0.72	3o24A	GO:0004659 GO:0009820 GO:0016740 GO:0050364
8	0.06	0.439	5.75	0.05	0.71	4e0tA	GO:0009820 GO:0016740 GO:0050364
9	0.06	0.440	5.44	0.09	0.68	4yzlA	GO:0009820 GO:0016740 GO:0050364
10	0.06	0.394	3.05	0.11	0.46	1m98A	GO:0005622 GO:0006810 GO:0007165 GO:0009579 GO:0009881 GO:0016020 GO:0016037 GO:0018298 GO:0030089 GO:0031404 GO:0042651 GO:0050896
11	0.06	0.402	2.82	0.14	0.45	4xb5A	GO:0005622 GO:0006810 GO:0007165 GO:0009579 GO:0009881 GO:0016020 GO:0016037 GO:0018298 GO:0030089 GO:0031404 GO:0042651 GO:0050896
12	0.06	0.327	5.93	0.03	0.55	1jofA	GO:0016853 GO:0017057 GO:0042952 GO:0047768
13	0.06	0.297	6.48	0.05	0.54	4i5jA	GO:0000159 GO:0001754 GO:0005509 GO:0006470 GO:0007525 GO:0008601 GO:0030674 GO:0034047 GO:0045732 GO:0046872 GO:0061053 GO:0090090 GO:0090244 GO:0090249 GO:0090263
14	0.06	0.286	5.80	0.04	0.47	1moeA
15	0.06	0.271	6.33	0.05	0.48	4ce0A	GO:0008168 GO:0016740 GO:0032259 GO:0046872
16	0.06	0.240	5.87	0.07	0.39	2l7bA	GO:0000302 GO:0001523 GO:0001540 GO:0001937 GO:0002021 GO:0005319 GO:0005543 GO:0005576 GO:0005615 GO:0005634 GO:0005737 GO:0005769 GO:0005783 GO:0005794 GO:0005886 GO:0006629 GO:0006641 GO:0006707 GO:0006810 GO:0006869 GO:0006874 GO:0006898 GO:0006979 GO:0007010 GO:0007186 GO:0007263 GO:0007271 GO:0008201 GO:0008202 GO:0008203 GO:0008289 GO:0010468 GO:0010544 GO:0010873 GO:0010875 GO:0015485 GO:0015909 GO:0016020 GO:0016209 GO:0017038 GO:0017127 GO:0019068 GO:0019433 GO:0019934 GO:0030195 GO:0030425 GO:0030516 GO:0030828 GO:0031012 GO:0031102 GO:0032489 GO:0032805 GO:0033344 GO:0033700 GO:0034361 GO:0034362 GO:0034363 GO:0034364 GO:0034372 GO:0034374 GO:0034375 GO:0034380 GO:0034382 GO:0034384 GO:0034447 GO:0042157 GO:0042158 GO:0042159 GO:0042311 GO:0042627 GO:0042632 GO:0042802 GO:0042803 GO:0043025 GO:0043407 GO:0043524 GO:0043537 GO:0043691 GO:0044794 GO:0045541 GO:0046889 GO:0046907 GO:0046911 GO:0048156 GO:0048168 GO:0048844 GO:0050728 GO:0050750 GO:0051000 GO:0051044 GO:0051055 GO:0051651 GO:0055088 GO:0055089 GO:0060228 GO:0060999 GO:0061000 GO:0070062 GO:0070326 GO:0071682 GO:0071813 GO:0072358 GO:0072562 GO:0090090 GO:0090370 GO:0097113 GO:0097114 GO:0098869 GO:1900221 GO:1901214 GO:1901215 GO:1901216 GO:1901627 GO:1901628 GO:1901630 GO:1901631 GO:1902004 GO:1902430 GO:1902947 GO:1902951 GO:1902952 GO:1902995 GO:1902998 GO:1902999 GO:1903001 GO:1903002 GO:1903561
17	0.06	0.246	6.37	0.04	0.44	1ww1A	GO:0016787 GO:0034414 GO:0042781 GO:0046872
18	0.06	0.209	4.85	0.05	0.30	2gtlB	GO:0005344 GO:0005506 GO:0005576 GO:0005833 GO:0006810 GO:0015671 GO:0019825 GO:0020037 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0004769
GO-Score	0.45
Biological Processes	GO:0008202	GO:0006810	GO:0017000
GO-Score	0.45	0.37	0.30
Cellular Component	GO:0005622
GO-Score	0.37

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.