I-TASSER results

I-TASSER results for job id Rv1920

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (287 residues)
MFPRWPQQAHNHEVSRADTVSVPRAPTQAEVAAVLRIMTPLRKVIKPKVYGIENVPTERA
LLVGNHNTLGLVDAPLLAAELWERGRIVRSLGDHAHFKIPGWRDALTRTGVVEGTREITS
ELMRRGELVMVFPGGAREVNKRKNERYKLVWKNRLGFARLAIQHGYPIVPFASVGAEHGI
DIVLDNESPLLAPVQFLAEKLLGTKDGPALVRGVGLTPVPRPERQYYWFGEPIDTTEFMG
QQADDNAARRVRERAAAAIEHGIELMLAERAADPNRSLVGRLLRSDA

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MFPRWPQQAHNHEVSRADTVSVPRAPTQAEVAAVLRIMTPLRKVIKPKVYGIENVPTERALLVGNHNTLGLVDAPLLAAELWERGRIVRSLGDHAHFKIPGWRDALTRTGVVEGTREITSELMRRGELVMVFPGGAREVNKRKNERYKLVWKNRLGFARLAIQHGYPIVPFASVGAEHGIDIVLDNESPLLAPVQFLAEKLLGTKDGPALVRGVGLTPVPRPERQYYWFGEPIDTTEFMGQQADDNAARRVRERAAAAIEHGIELMLAERAADPNRSLVGRLLRSDA
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEHCHCCCCCCEEEEHCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEHCCCHCCCCCCCCEEEEEHCCCCHHHHHHHHHCCCEEEEEEHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
Conf.Score	99887655545554457777788898689999999999999885455999844689998799986898757899999998757898578876356655776999999989966899999999997998999959844577788985789877985478888998999899998755444455468886566788889999877887776555666678889966999988985676678999999999999999999999999999999856888557777776559
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MFPRWPQQAHNHEVSRADTVSVPRAPTQAEVAAVLRIMTPLRKVIKPKVYGIENVPTERALLVGNHNTLGLVDAPLLAAELWERGRIVRSLGDHAHFKIPGWRDALTRTGVVEGTREITSELMRRGELVMVFPGGAREVNKRKNERYKLVWKNRLGFARLAIQHGYPIVPFASVGAEHGIDIVLDNESPLLAPVQFLAEKLLGTKDGPALVRGVGLTPVPRPERQYYWFGEPIDTTEFMGQQADDNAARRVRERAAAAIEHGIELMLAERAADPNRSLVGRLLRSDA
Prediction	74342444154443453344334441232102200311330330120302311412752000000011012200000010023461201000232034010011103301001012620340065420000003101212434544120214622000200341701000000022340042144453122220342033114031021313222013033333000001321406626567414520440263026223500550275247336241032123558
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEHCHCCCCCCEEEEHCCCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEHCCCHCCCCCCCCEEEEEHCCCCHHHHHHHHHCCCEEEEEEHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
MFPRWPQQAHNHEVSRADTVSVPRAPTQAEVAAVLRIMTPLRKVIKPKVYGIENVPTERALLVGNHNTLGLVDAPLLAAELWERGRIVRSLGDHAHFKIPGWRDALTRTGVVEGTREITSELMRRGELVMVFPGGAREVNKRKNERYKLVWKNRLGFARLAIQHGYPIVPFASVGAEHGIDIVLDNESPLLAPVQFLAEKLLGTKDGPALVRGVGLTPVPRPERQYYWFGEPIDTTEFMGQQADDNAARRVRERAAAAIEHGIELMLAERAADPNRSLVGRLLRSDA

1k30A2

0.13

0.11

0.80

1.41

wdPPAS

--------SSHHKAIREPFD---------YYIFGQNYIRPLIDFGNSFVGNLEKLQQGHVVLISNHQT---ADPAIISLLLEKTNENTIFVAGDRVLADPLCKPFSIGRNLICVSLKEMALLLRGGQLIWIAPSGGRDRPDPSTGEWYPA-SSVDNMRRLIQHSDVPLFPLALLC----HDIMPPPS---------QVEIEIRV---------IAFNGA------GLSVAPEISFEEIAATHKNEEVREAYSKALFDSVAMQYNVLKTAISGK-------QGLGAST

1k30A2

0.14

0.11

0.78

1.28

wPPAS

-SS-------HHKAIREPFD----------------YYIFGQNYIR-FVGNLEKLQQGNVVLISNHQT---ADPAIISLLLEKTNENTIFVAGDRVLADPLCKPFSIGRNLICVSLKEMALLLRGGQLIWIAPSGGRDRPDPSTGEWYP--SSVDNMRRLIQHSDVPLFPLALLC----HDIMPPPS-----QVEIEIR-------------------VIAFNGAGLSVAPEISFEEIAATHKNEEVREAYSKALFDSVAMQYNVLKTAISGK--QGLGAST---AD

2mr6A

0.15

0.06

0.40

2.10

dPPAS2

-----------------------------------------------------------------------------------MGVVILVLTGDERIAE-ELRKEVQKHDTVPTKKEEIEKARKQGRPIVVFVRGGDDERA-------------KDIAEYAQKEGLRVIVIIVSQDEEALRKGYRNEDEAKKKLKEALEKSGSLEHHHHHH----------------------------------------------------------------------------

1k30A

0.14

0.11

0.82

1.07

wdPPAS

EDPF--VFSSHHKAIREPFD---------YYIFGQNYIRPLIDFGNSFVGNLEKLQQGHVVLISNHQT---ADPAIISLLLEKTNENTIFVAGDRVLADPLCKPFSIGRNLICVSLKEMALLLRGGQLIWIAPSGGRDRPDPSTGEWYP--SSVDNMRRLIQHSDVPLFPLALLC----HDIMPPPS---------QVEIEIRV---------IAFNGA------GLSVAPEISFEEIAATHKNEEVREAYSKALFDSVAMQYNVLKTAISGK-------QGLGAST

4b6gA

0.11

0.07

0.62

1.68

dPPAS2

PNDDAYDLGQSAGFYLNATEQPWAANYQMYDYILNELPRLIEKHFP---------TNGKRSIMG----HSMGGHGALVLALRNQERYQSVSAFSPSLVPWGEKAFTAYLGKDREKQYDANSLIQQGYQGMRIDQGLEDEFLPTQLRTE-------DFIETCRAANQPVDVRFHKGYDHSYYFIA----SFIGEHIAYHAAFLK------------------------------------------------------------------------------------

4j29A

0.20

0.08

0.40

1.61

dPPAS2

----------------------------------------------------------TVVIVV--SRDERILEELLEVVLKSDPNVKTVRTDD-----------KEKV------KEEIEKARKQGRPIVIFIRGATEEVVR-------------DIVEYAQKEGLRVLVIVDQDQEELERIYEDNEDEAKKRLKELLEKVLEH-----------------------------------------------------------------------------------

2exxA1

0.12

0.05

0.45

1.61

dPPAS2

---------------------------------------------------------------------LAQGGSVARTLLEDGTFKVRVVTRNP---RKKAAKELRLQGAEVVDQVIMELALNGAYATFIV------TNYWESCSQEQEVKQGKLLADLARRLGLHVVYSGLENIKKLTAGRLAAAHFDGKGEVEEYFRDIGVPMT--------------------------------------------------------------------------------

4m8aA

0.12

0.02

0.21

1.53

dPPAS2

---------------------------------------------------------------------------------------------------------------SKLSYTSFVQMVEDEREVVIRDDGVLRVYTKDGRVYEVDAVNDSQLIEKLVSKGI-----KVSGE---------------------------------------------------------------------------------------------------------------

3irsA

0.12

0.10

0.84

0.66

MUSTER

PAMGFLNARIYTRPDIRNRFTRQLGFEPAPSAEEKSLELMFEEM-----AGI-----EQGVCVGRNSSVLVSNADVAAVAKAYPDKFHPVGS------IE--------AATRKEAMAQMQEILDLGRIVNLEPGVWATPMHVDDRRLY-------PLYAFCEDNGIPVIMMTGGNAG------PDITYTNPEHIDRVLGDFPDLTVVSSHGNWPWVQEIIRPN-LYLSPD----LYNLPGHADFIQAANSFLKEYTEWFLTL-PDAMEKIL----HGNAERLLAQAG

5bq5A

0.09

0.05

0.57

0.71

dPPAS

------------RKTIDTFDFTAQPSVDERRIRELLTLSFID--------------RKENILFLGPPGIGKTHLAISIGEAIARGYKTYFIT---AHDLVNQLRRADQEG----KLEKKLRVFVKPTVLIIDEGYLKLDPNSAHYL---------FQVIARRYEHAPIILTSNKSFGEWGEIVGDSVLATALDRLLHHSIIFNLKGE--------------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.78

Estimated TM-score = 0.50±0.15

Estimated RMSD = 10.2±4.6Å

Download Model 2

C-score=-5.00

Download Model 3

C-score=-2.21

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1k30A	0.736	2.94	0.105	0.854
2	5hm8A	0.500	5.49	0.068	0.784
3	3ondA	0.499	5.65	0.075	0.791
4	3h9uC	0.492	5.58	0.103	0.780
5	3ce6A	0.490	5.64	0.092	0.780
6	3glqA	0.484	5.60	0.070	0.767
7	1v8bA	0.481	5.64	0.100	0.763
8	3gvpA	0.480	5.90	0.054	0.780
9	3n58A	0.474	5.64	0.088	0.753
10	5kf6A	0.469	5.86	0.059	0.770

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.07	3	2x2vH	DPV	Rep, Mult	255,263
2	0.07	3	2x2vB	DPV	Rep, Mult	259,263,266,270
3	0.05	2	3a0bK	CLA	Rep, Mult	167,168,170
4	0.05	2	3bk9C	TRP	Rep, Mult	252,255
5	0.02	1	3qs0D	NAG	Rep, Mult	59,165
6	0.02	1	2ofwA	ADX	Rep, Mult	54,55
7	0.02	1	1r9cB	MN	Rep, Mult	66,68
8	0.02	1	2zyqB	TAR	Rep, Mult	133,134
9	0.02	1	2onkE	MG	Rep, Mult	83,100,104
10	0.02	1	3mtgB	NAD	Rep, Mult	69,91,92,93,94,97,133,134,154
11	0.02	1	3phxA	ZN	Rep, Mult	230,234
12	0.02	1	3jpyA	ZN	Rep, Mult	66,231
13	0.02	1	3h9uB	NAD	Rep, Mult	119,161,166
14	0.02	1	1iuqA	GOL	Rep, Mult	148,149,151,249,252,253,256
15	0.02	1	1llrD	UUU	Rep, Mult	135,137
16	0.02	1	1m34A	SF4	Rep, Mult	62,63

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1hwxA	0.424	5.92	0.077	0.679	1.4.1.3	NA
2	0.060	1z45A	0.417	5.03	0.111	0.603	5.1.3.2,5.1.3.3	NA
3	0.060	1v8bC	0.480	5.62	0.095	0.763	3.3.1.1	180,185
4	0.060	1kzhB	0.439	5.54	0.057	0.679	2.7.1.90	NA
5	0.060	2pffD	0.433	5.85	0.043	0.690	2.3.1.86	70,74
6	0.060	2pffA	0.433	5.67	0.048	0.679	2.3.1.41,2.3.1.86	NA
7	0.060	1v8bA	0.481	5.64	0.100	0.763	3.3.1.1	NA
8	0.060	1wvgA	0.413	5.35	0.091	0.613	4.2.1.45	NA
9	0.060	3m07A	0.413	6.32	0.077	0.693	3.2.1.141	132,133
10	0.060	3d64A	0.477	5.59	0.095	0.756	3.3.1.1	NA
11	0.060	3g1uC	0.482	5.36	0.077	0.749	3.3.1.1	NA
12	0.060	3ifgA	0.404	5.90	0.034	0.638	1.2.1.16	231
13	0.060	3k92A	0.416	6.19	0.045	0.700	1.4.1.2	139
14	0.060	1kerB	0.414	5.43	0.079	0.610	4.2.1.46	113
15	0.060	1i24A	0.356	5.88	0.056	0.582	3.13.1.1	NA
16	0.060	2o2rC	0.412	6.13	0.043	0.672	1.5.1.6	NA
17	0.060	3n58A	0.474	5.64	0.088	0.753	3.3.1.1	93,183
18	0.060	2ph5A	0.417	5.58	0.091	0.641	2.5.1.44	131
19	0.060	1k30A	0.736	2.94	0.105	0.854	2.3.1.15	66

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.13	0.736	2.94	0.10	0.85	1k30A	GO:0004366 GO:0006629 GO:0006650 GO:0008152 GO:0008654 GO:0009507 GO:0009536 GO:0009570 GO:0016024 GO:0016740 GO:0016746
1	0.07	0.501	5.54	0.09	0.79	5jpiA	GO:0004013 GO:0006730 GO:0016787
2	0.07	0.481	5.64	0.10	0.76	1v8bA	GO:0004013 GO:0005829 GO:0006730 GO:0016787 GO:0033353
3	0.07	0.490	5.64	0.09	0.78	3ce6A	GO:0004013 GO:0005576 GO:0005618 GO:0005737 GO:0005829 GO:0005886 GO:0006730 GO:0009087 GO:0016787 GO:0033353 GO:0040007 GO:0046085 GO:0070403
4	0.07	0.495	5.67	0.07	0.79	3ondB	GO:0004013 GO:0006730 GO:0016787
5	0.07	0.480	5.74	0.09	0.77	1a7aA	GO:0000096 GO:0001666 GO:0002439 GO:0004013 GO:0005634 GO:0005737 GO:0005829 GO:0006730 GO:0007584 GO:0016787 GO:0019510 GO:0030554 GO:0032259 GO:0033353 GO:0042470 GO:0042745 GO:0042802 GO:0043005 GO:0051287 GO:0070062 GO:0071268
6	0.07	0.477	5.59	0.10	0.76	3d64A	GO:0004013 GO:0005737 GO:0006730 GO:0016787
7	0.07	0.492	5.58	0.10	0.78	3h9uC	GO:0000166 GO:0004013 GO:0005829 GO:0006730 GO:0016787 GO:0033353
8	0.07	0.476	5.57	0.08	0.76	3n58B	GO:0004013 GO:0005737 GO:0006730 GO:0016787
9	0.07	0.480	5.90	0.05	0.78	3gvpA	GO:0004013 GO:0005737 GO:0005783 GO:0005829 GO:0006730 GO:0016787 GO:0033353 GO:0043005 GO:0043231
10	0.07	0.482	5.36	0.08	0.75	3g1uC	GO:0000166 GO:0004013 GO:0005829 GO:0006730 GO:0016787 GO:0033353
11	0.07	0.444	5.64	0.09	0.70	3x2fA	GO:0004013 GO:0005737 GO:0005829 GO:0006730 GO:0016787 GO:0033353
12	0.06	0.342	5.89	0.06	0.54	4ijuB	GO:0003845 GO:0005783 GO:0005789 GO:0006629 GO:0006704 GO:0008202 GO:0016020 GO:0016021 GO:0016491 GO:0030324 GO:0055114 GO:0070524
13	0.06	0.354	6.12	0.05	0.59	3vpxB	GO:0006520 GO:0016491 GO:0016639 GO:0055114
14	0.06	0.373	4.92	0.12	0.53	3d3wB	GO:0005634 GO:0005881 GO:0005886 GO:0005902 GO:0005903 GO:0005975 GO:0005997 GO:0006006 GO:0006739 GO:0016020 GO:0016491 GO:0016614 GO:0016655 GO:0019640 GO:0042732 GO:0050038 GO:0051289 GO:0055114 GO:0070062
15	0.06	0.368	5.85	0.05	0.57	5a2aA	GO:0003824 GO:0004556 GO:0005975 GO:0008152 GO:0016020 GO:0016021 GO:0016787 GO:0016798
16	0.06	0.388	5.11	0.12	0.56	3awdA	GO:0016491 GO:0055114
17	0.06	0.340	6.10	0.04	0.57	5dt9A	GO:0004617 GO:0005737 GO:0008152 GO:0008615 GO:0009070 GO:0016491 GO:0016616 GO:0033711 GO:0046983 GO:0051287 GO:0055114
18	0.06	0.343	5.07	0.07	0.52	3dttB	GO:0000166

Consensus prediction of GO terms

Molecular Function	GO:0016802
GO-Score	0.48
Biological Processes	GO:0044281	GO:0044763	GO:0044237
GO-Score	0.48	0.48	0.40
Cellular Component	GO:0005829	GO:0009570	GO:0005886	GO:0005618	GO:0005576
GO-Score	0.13	0.13	0.07	0.07	0.07

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.