I-TASSER results

I-TASSER results for job id Rv1836c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (677 residues)
MGRHSKPDPEDSVDDLSDGHAAEQQHWEDISGSYDYPGVDQPDDGPLSSEGHYSAVGGYS
ASGSEDYPDIPPRPDWEPTGAEPIAAAPPPLFRFGHRGPGDWQAGHRSADGRRGVSIGVI
VALVAVVVMVAGVILWRFFGDALSNRSHTAAARCVGGKDTVAVIADPSIADQVKESADSY
NASAGPVGDRCVAVAVTSAGSDAVINGFIGKWPTELGGQPGLWIPSSSISAARLTGAAGS
QAISDSRSLVISPVLLAVRPELQQALANQNWAALPGLQTNPNSLSGLDLPAWGSLRLAMP
SSGNGDAAYLAGEAVAAASAPAGAPATAGIGAVRTLMGARPKLADDSLTAAMDTLLKPGD
VATAPVHAVVTTEQQLFQRGQSLSDAENTLGSWLPPGPAAVADYPTVLLSGAWLSQEQTS
AASAFARYLHKPEQLAKLARAGFRVSDVKPPSSPVTSFPALPSTLSVGDDSMRATLADTM
VTASAGVAATIMLDQSMPNDEGGNSRLSNVVAALENRIKAMPPSSVVGLWTFDGREGRTE
VPAGPLADPVNGQPRPAALTAALGKQYSSGGGAVSFTTLRLIYQEMLANYRVGQANSVLV
ITAGPHTDQTLDGPGLQDFIRKSADPAKPIAVNIIDFGADPDRATWEAVAQLSGGSYQNL
ETSASPDLATAVNIFLS

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MGRHSKPDPEDSVDDLSDGHAAEQQHWEDISGSYDYPGVDQPDDGPLSSEGHYSAVGGYSASGSEDYPDIPPRPDWEPTGAEPIAAAPPPLFRFGHRGPGDWQAGHRSADGRRGVSIGVIVALVAVVVMVAGVILWRFFGDALSNRSHTAAARCVGGKDTVAVIADPSIADQVKESADSYNASAGPVGDRCVAVAVTSAGSDAVINGFIGKWPTELGGQPGLWIPSSSISAARLTGAAGSQAISDSRSLVISPVLLAVRPELQQALANQNWAALPGLQTNPNSLSGLDLPAWGSLRLAMPSSGNGDAAYLAGEAVAAASAPAGAPATAGIGAVRTLMGARPKLADDSLTAAMDTLLKPGDVATAPVHAVVTTEQQLFQRGQSLSDAENTLGSWLPPGPAAVADYPTVLLSGAWLSQEQTSAASAFARYLHKPEQLAKLARAGFRVSDVKPPSSPVTSFPALPSTLSVGDDSMRATLADTMVTASAGVAATIMLDQSMPNDEGGNSRLSNVVAALENRIKAMPPSSVVGLWTFDGREGRTEVPAGPLADPVNGQPRPAALTAALGKQYSSGGGAVSFTTLRLIYQEMLANYRVGQANSVLVITAGPHTDQTLDGPGLQDFIRKSADPAKPIAVNIIDFGADPDRATWEAVAQLSGGSYQNLETSASPDLATAVNIFLS
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHCCHHHHHHHHHHCCCCCCCCCCCEEHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHCCCCCCHCCCCEEEHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHCCCCCEEEHCCEHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHCCCCCHHHHHHHHHHHCCEEEHCCCCCHHHHHHHHHHHHC
Conf.Score	98888899887777888888877778888888777888878778888877766766778888777778888888888888888888888888888888887776667888888654789999999999999999997646666777777777888886799999777589999999999998688768856999999958999999998667877788885775787799999998667666777887455556787568899986678899998886577655555677766556568888888678999999986678888875567899999998666678887688999876577888877665445599999998567888887699967998654568679867888899999999999999968899999999878888889888777666666666666655567777776655676658999966899988888778999999999999988999789999987987589866655677888866999999999985678898758999999999999858888875999997899999999999999999998788998689999868999999999999998997998799758999999999869
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 600 620 640 660 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MGRHSKPDPEDSVDDLSDGHAAEQQHWEDISGSYDYPGVDQPDDGPLSSEGHYSAVGGYSASGSEDYPDIPPRPDWEPTGAEPIAAAPPPLFRFGHRGPGDWQAGHRSADGRRGVSIGVIVALVAVVVMVAGVILWRFFGDALSNRSHTAAARCVGGKDTVAVIADPSIADQVKESADSYNASAGPVGDRCVAVAVTSAGSDAVINGFIGKWPTELGGQPGLWIPSSSISAARLTGAAGSQAISDSRSLVISPVLLAVRPELQQALANQNWAALPGLQTNPNSLSGLDLPAWGSLRLAMPSSGNGDAAYLAGEAVAAASAPAGAPATAGIGAVRTLMGARPKLADDSLTAAMDTLLKPGDVATAPVHAVVTTEQQLFQRGQSLSDAENTLGSWLPPGPAAVADYPTVLLSGAWLSQEQTSAASAFARYLHKPEQLAKLARAGFRVSDVKPPSSPVTSFPALPSTLSVGDDSMRATLADTMVTASAGVAATIMLDQSMPNDEGGNSRLSNVVAALENRIKAMPPSSVVGLWTFDGREGRTEVPAGPLADPVNGQPRPAALTAALGKQYSSGGGAVSFTTLRLIYQEMLANYRVGQANSVLVITAGPHTDQTLDGPGLQDFIRKSADPAKPIAVNIIDFGADPDRATWEAVAQLSGGSYQNLETSASPDLATAVNIFLS
Prediction	73424322264534435544244444445443435224553455334346252245442445445632534444424444443434243232433434345232322434321100000000000000010010000001332444444434512635130201001401410450054047553415321020204334134004202543446344211010142110022034534543344321001010002024400640442314302422344431441431321202112223310100000000002334455443441242044035334424442223024212444432322120000002001320443553522010000451001000000002344346423400430141033450242024322223321112222221221111110111112221222222222120000000101235443320041024003400640366000000000142232211012013425444224302520453344311110030023005203742566200000000001033442425401520463455723010000000142036004200730413123043551340151044017
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580                 600                 620                 640                 660

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Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHCCHHHHHHHHHHCCCCCCCCCCCEEHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEHCCCCCCHCCCCEEEHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHCCCCCEEEHCCEHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEHCCCCCHHHHHHHHHHHCCEEEHCCCCCHHHHHHHHHHHHC
MGRHSKPDPEDSVDDLSDGHAAEQQHWEDISGSYDYPGVDQPDDGPLSSEGHYSAVGGYSASGSEDYPDIPPRPDWEPTGAEPIAAAPPPLFRFGHRGPGDWQAGHRSADGRRGVSIGVIVALVAVVVMVAGVILWRFFGDALSNRSHTAAARCVGGKDTVAVIADPSIADQVKESADSYNASAGPVGDRCVAVAVTSAGSDAVINGFIGKWPTELGGQPGLWIPSSSISAARLTGAAGSQAISDSRSLVISPVLLAVRPELQQALANQNWAALPGLQTNPNSLSGLDLPAWGSLRLAMPSSGNGDAAYLAGEAVAAASAPAGAPATAGIGAVRTLMGARPKLADDSLTAAMDTLLKPGDVATAPVHAVVTTEQQLFQRGQSLSDAENTLGSWLPPGPAAVADYPTVLLSGAWLSQEQTSAASAFARYLHKPEQLAKLARAGFRVSDVKPPSSPVTSFPALPSTLSVGDDSMRATLADTMVTASAGVAATIMLDQSMPNDEGGNSRLSNVVAALENRIKAMPPSSVVGLWTFDGREGRTEVPAGPLADPVNGQPRPAALTAALGKQYSSGGGAVSFTTLRLIYQEMLANYRVGQANSVLVITAGPHTDQTLDGPGLQDFIRKSADPAKPIAVNIIDFGADPDRATWEAVAQLSGGSYQNLETSASPDLATAVNIFLS

5gaoE

0.17

0.09

0.50

1.14

dPPAS2

-------------------------------------------------------------------------------------------------------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-------------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

4fx5A1

0.14

0.04

0.26

1.18

wdPPAS

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SENVEVIIIDCSGSDYP----RTKAAKEATKVAIDTLTDGAFFAVVAGTEGA-RVVYPTG--GQLLRADYQSRAAAKEAVGRLHANGGTAGRWLAQAGRIFDTA---PSAIKHAILLTDGKDESE--TPADLARAIQSS---IGNFTADCRGIGEDWEPKELRKIADALLGTVGIIRD--PATLAEDFRETAK

1sbpA

0.12

0.05

0.44

1.14

dPPAS2

------------------------------------------------------------------------------------------------------------------------------------------------------------KDIQLLNVSYDPTRELYEQYNKAFSAHWKQETGDNVVIDQSHGGSGKQATSVINGIEA------DTVTLALAYDVNAIAERGWIKRLPDDSAPYTSTIVFLVRK------------------GNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAWGYALHHNNNDQAKAEDFVKALFKNVEVLDSGARGSTNTFVERGI-----GDVLIAWENEALLATNEL--GKDKFEIVTPSESI-LAEPTVSVVDKVVEKKDTKAVAEAYLKYLYSPEGQEIAAKNFYRPRDADVAKKYDDAFPKLKLFTIDEVFGGWAKAQKDHFADGGK-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

4fx5A1

0.16

0.04

0.26

1.69

wPPAS

----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SENVEVIIIDCSGSDYP----RTKAAKEATKVAIDTLTDGAFFAVVAGTEGA-RVVYPTGGQLLRADY-QSRAAAKEAVGR-LHANGGTAGRWLAQAGRIFDTA---PSAIKHAILLTDGKDESE--TPADLARAIQSS---IGNFTADCRGIGEDWEPKELRKIADALLGTVGIIRD--PATLAEDFRETAS

5b7iA

0.11

0.10

0.94

0.86

MUSTER

AYRHEFVGPGSSDEDWLRRLADKRETGDAWLSQLARDDRQSAPPGPFQKSRLPPNGDHRGSASLARLPAPIQSQWCGARDADAKEKAACWQFPHGLFASAHWRARTASLERPGLLARGPALLHDSYVHVSRLILLADHHYSSLPADSRLGDPNFPSGKLKQRLLGVALHSRKLAGTLPRLERQLPRLARHGFTRRVEQPRFRWQDKAYDCAACREQAEHGFFGLNLNGRILYALADPQRG-----ARFSIALGLTLQTGQAYRERLGLGDDDLAIELLAENSHVHFAGTLEDGPLREWLGGNSAGNRLLQAIDHLPASESLRGGHQIAPLLRLTSDVDDFDIDDLPALSRLVHWAGLFGSRPPALVQGLFEAYRSGREIFQRHRGAPGRATEIR----CAWFDEFSSQSSAHGAVTSFSEAHATFVAQRLAKLEQ----LPPRRQAQLCTVHAAGEARPALCRELAGQNTWADLHRCHHTEHQGRRISFGLLR----------LANIEPLIELAQAILAQGPEGLHVHLCVYHSRHPL---------------LVRSAIERQLDELLKRSDDDAAALFARPTLAKALQASTERDHLFVVLASDYDWAIVEPSSRSIIQLAGSGFSGEANLYLLSRNIGQNPDPALLAASPRIVEPFPLFPRSRLVDLEHRRLRALLA

5gaoE

0.17

0.09

0.50

0.69

dPPAS

-------------------------------------------------------------------------------------------------------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-------------------------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA---------AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

5b7iA

0.12

0.11

0.94

0.85

wMUSTER

IGRHEFVGPGSSDEDWLRRLADKRETGDAWLSQLARDDRQSAPPGPFQKSRLPPNGDHRGSASLARLPAPIQSQWCGARDADAKEKAACWQFPHGLFASAHWRARTASLERPGLLARGPALLHDSYVHVSRLILLADHHYSSLPADSRLGDPNFPSGKLKQRLLGVALHSRKLAGTLPRLERQLPRLARKGFTRRVEQPRFRWQDKAYDCAACREQAEHGFFGL-ANGRILYALADPQRG-----ARFSIALGLTLQTGQAYRERLGLGD-DDLAELLAENSHVHFAGTLEDGPLREWLGGNSAGNRLLQAIDHLPASESLRGGHQIAPLLRLTSD--EVDDFDIDDLPALSRLVHWAGLFGSRVLSATPALVQGLFEAYRSG----REIFQRHRGAPGRATEIRCAWFDEFSSQSSAHGAVTSFSEAHATAQRLAKLEQLPPRRQAQLCTVHAAGEARPALCRELAGQNTWADLHRCHHTEHQGRRISFGLLRLA---------NIEPLIELAQAILAQGAPEGHVHLCVYHSRHPL---------------LVRSAIERQLDELLKRSDDDAAALFARPTLAKALQASTERDHLFVVLAS-GRDHD-EPSSRSIIQLAGSGFSGEANLYLLSRNIGQNPDPALLAASPRIVEPFPLFPRSRLVDLEHRRLRALLA

5a8jA1

0.12

0.03

0.24

1.19

dPPAS2

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGIHYVVMIDNSPSMK----KENKINLALSSASRLVQDIIPGNFISIYLFSNDI-ETLYEGESGKQIELKS-------------IKMGYTTNLHKAITKVLEKFKSS---EIPVKIILLSDGKPTDKRYSRDYE------SLQVPKNVQLITIGLGEDYNEAIMKILADKGSGVFYHIND------PSQLPTTLV

5a8jA1

0.12

0.03

0.24

1.37

PPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGIHYVVMIDNSPSMK----KENKINLALSSASRLVQDIIPGNFISIYLFSNDI-ETLYEGESGKQ------------IELKSI-KMGYTTNLHKAITKVLEKFK---SSEIPVKIILLSDGKPTDKRY----SRDYESLQVP--KNVQLITIGLGEDYNEAIMKILADKGSGVFYHIND------PSQLPTTLV

5a8jA1

0.12

0.03

0.24

2.79

Env-PPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGIHYVVMIDNSPSMK----KENKINLALSSASRLVQDIIPGNFISIYLFSNDI-ETLYEGESGKQ------------IELKSIK-MGYTTNLHKAITKVLEKFK---SSEIPVKIILLSDGKPTDKRY----SRDYESLQVP--KNVQLITIGLGEDYNEAIMKILADKGSGVFYHIND------PSQLPTTLV

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.22

Estimated TM-score = 0.35±0.12

Estimated RMSD = 16.2±3.1Å

Download Model 2

C-score=-4.08

Download Model 3

C-score=-2.41

Download Model 4

C-score=-2.47

Download Model 5

C-score=-2.69

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4wrnA	0.436	5.83	0.091	0.561
2	1sbpA	0.418	2.71	0.098	0.446
3	5eduB	0.417	5.71	0.087	0.536
4	4bl8A	0.412	6.08	0.101	0.541
5	4h1gA	0.399	6.37	0.088	0.532
6	4aq4A	0.391	5.00	0.077	0.479
7	4ryaA	0.388	5.13	0.062	0.480
8	3dm0A	0.386	5.62	0.102	0.487
9	5a22A	0.386	8.18	0.050	0.628
10	1eu8A	0.385	5.08	0.057	0.470

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.15	7	2h5yA	MOO	Rep, Mult	166,167,168,200,201,305,306,307,347,348,372
2	0.08	4	5t1pA	GPE	Rep, Mult	165,199,200,201,223,305,306,307,347,404
3	0.08	4	3k6sA	MG	Rep, Mult	494,496,498,603
4	0.04	2	1shtX	MG	Rep, Mult	496,498,573
5	0.02	1	2wpnA	SBY	Rep, Mult	489,491,598,600,672,675
6	0.02	1	4eqbA	CA	Rep, Mult	183,434
7	0.02	1	1xvxA	CO3	Rep, Mult	296,360
8	0.02	1	1l8tA	MG	Rep, Mult	484,636
9	0.02	1	3tazB	MN	Rep, Mult	494,515,533
10	0.02	1	3ihkA	MG	Rep, Mult	640,642
11	0.02	1	1xeyA	GUA	Rep, Mult	531,532
12	0.02	1	1xzoA	CA	Rep, Mult	531,532,638

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2ihvA	0.322	7.72	0.070	0.508	2.5.1.66	NA
2	0.060	2vdcF	0.330	8.20	0.048	0.538	1.4.1.13	601
3	0.060	2ipyA	0.276	7.80	0.040	0.436	4.2.1.3	NA
4	0.060	1g8kA	0.327	8.67	0.039	0.557	1.20.98.1	NA
5	0.060	1ozgA	0.317	7.48	0.071	0.487	2.2.1.6	NA
6	0.060	2nyaF	0.330	7.80	0.054	0.520	1.7.99.4	NA
7	0.060	1ybhA	0.328	7.24	0.053	0.495	2.2.1.6	NA
8	0.060	1b0pA	0.318	7.72	0.041	0.490	1.2.7.1	NA
9	0.060	2thiA	0.340	4.67	0.073	0.415	2.5.1.2	NA
10	0.060	3ecqB	0.325	8.31	0.031	0.542	3.2.1.97	NA
11	0.060	2e7zA	0.315	8.06	0.056	0.504	4.2.1.71	589
12	0.060	2vbiA	0.314	7.78	0.074	0.496	4.1.1.1	NA
13	0.060	2r7oA	0.330	8.87	0.029	0.575	2.7.7.48	NA
14	0.060	2v3wA	0.320	7.69	0.069	0.504	4.1.1.7	NA
15	0.060	1t3tA	0.349	8.28	0.042	0.584	6.3.5.3	NA
16	0.060	1kqfA	0.321	8.34	0.058	0.538	1.2.1.2	NA
17	0.060	1h0hA	0.332	7.76	0.037	0.520	1.2.1.2	NA
18	0.060	2pdaA	0.291	8.14	0.029	0.467	1.2.7.1	493,525,634
19	0.060	1n1hA	0.340	8.74	0.033	0.581	2.7.7.48	516

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.07	0.416	5.41	0.08	0.52	3mp8A	GO:0000124 GO:0001135 GO:0005215 GO:0005363 GO:0005634 GO:0005671 GO:0006351 GO:0006355 GO:0006810 GO:0006974 GO:0008643 GO:0015768 GO:0016568 GO:0030288 GO:0034289 GO:0034613 GO:0034629 GO:0035064 GO:0042597 GO:0042956 GO:0043190 GO:0043970 GO:0043971 GO:0044154 GO:0045944 GO:0055052 GO:0060326 GO:0070868 GO:1901982 GO:1990060
1	0.06	0.436	5.83	0.09	0.56	4wrnA	GO:0000922 GO:0005215 GO:0005363 GO:0005509 GO:0005576 GO:0005615 GO:0005794 GO:0005886 GO:0005929 GO:0006810 GO:0006968 GO:0007157 GO:0007159 GO:0007588 GO:0008285 GO:0008643 GO:0010033 GO:0015768 GO:0016020 GO:0016323 GO:0016324 GO:0019864 GO:0019898 GO:0031225 GO:0031410 GO:0042597 GO:0042995 GO:0045121 GO:0045177 GO:0048878 GO:0060170 GO:0070062 GO:0072218 GO:0072221 GO:0072233 GO:0072372 GO:1990266 GO:2000021
2	0.06	0.410	6.05	0.11	0.54	4blbB	GO:0000122 GO:0001501 GO:0001843 GO:0001947 GO:0003281 GO:0003714 GO:0004871 GO:0005215 GO:0005363 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005929 GO:0006355 GO:0006508 GO:0006810 GO:0006974 GO:0007165 GO:0007275 GO:0007368 GO:0008013 GO:0008134 GO:0008643 GO:0015768 GO:0019901 GO:0021513 GO:0021775 GO:0021776 GO:0030288 GO:0034289 GO:0035904 GO:0042597 GO:0042956 GO:0042992 GO:0042994 GO:0043190 GO:0043433 GO:0043588 GO:0045668 GO:0045879 GO:0055052 GO:0060326 GO:0060976 GO:0072372 GO:0097542 GO:0097546 GO:1901621 GO:1901982 GO:1990060 GO:2000059
3	0.06	0.407	5.31	0.09	0.51	5dfmA	GO:0005215 GO:0005363 GO:0006810 GO:0006974 GO:0008643 GO:0015768 GO:0030288 GO:0034289 GO:0042597 GO:0042956 GO:0043190 GO:0055052 GO:0060326 GO:1901982 GO:1990060
4	0.06	0.404	5.83	0.08	0.52	4xa2A	GO:0005215 GO:0005363 GO:0006810 GO:0008643 GO:0015768 GO:0042597
5	0.06	0.410	5.56	0.08	0.52	4egcA	GO:0000122 GO:0000978 GO:0001077 GO:0001657 GO:0001658 GO:0001759 GO:0001822 GO:0003151 GO:0003677 GO:0003682 GO:0003700 GO:0005215 GO:0005363 GO:0005634 GO:0005667 GO:0005730 GO:0005737 GO:0006351 GO:0006355 GO:0006366 GO:0006810 GO:0006915 GO:0006974 GO:0007275 GO:0007389 GO:0007519 GO:0007605 GO:0008283 GO:0008582 GO:0008643 GO:0010468 GO:0014842 GO:0015768 GO:0021610 GO:0022008 GO:0030288 GO:0030855 GO:0030878 GO:0030910 GO:0032880 GO:0034289 GO:0034504 GO:0035909 GO:0042472 GO:0042474 GO:0042597 GO:0042956 GO:0043066 GO:0043190 GO:0043524 GO:0043565 GO:0043586 GO:0044212 GO:0045664 GO:0045893 GO:0045944 GO:0048513 GO:0048538 GO:0048665 GO:0048699 GO:0048701 GO:0048704 GO:0048705 GO:0048839 GO:0048856 GO:0050678 GO:0051451 GO:0055052 GO:0060037 GO:0060326 GO:0061055 GO:0061197 GO:0061551 GO:0071599 GO:0072001 GO:0072075 GO:0072095 GO:0072107 GO:0072172 GO:0072193 GO:0072513 GO:0090103 GO:0090190 GO:0090191 GO:1901982 GO:1990060 GO:2000729 GO:2001014
6	0.06	0.397	5.65	0.09	0.51	3py7A	GO:0001725 GO:0003677 GO:0005178 GO:0005215 GO:0005363 GO:0005654 GO:0005737 GO:0005829 GO:0005856 GO:0005875 GO:0005886 GO:0005925 GO:0005938 GO:0006351 GO:0006355 GO:0006810 GO:0006936 GO:0006974 GO:0007155 GO:0007160 GO:0007165 GO:0007172 GO:0007173 GO:0007179 GO:0008013 GO:0008270 GO:0008643 GO:0015768 GO:0016032 GO:0017166 GO:0019901 GO:0030027 GO:0030054 GO:0030288 GO:0032403 GO:0034289 GO:0034614 GO:0042025 GO:0042597 GO:0042956 GO:0043190 GO:0044204 GO:0046872 GO:0048010 GO:0055052 GO:0060326 GO:0060396 GO:1901982 GO:1990060
7	0.06	0.399	6.37	0.09	0.53	4h1gA	GO:0000166 GO:0003777 GO:0005215 GO:0005363 GO:0005524 GO:0005874 GO:0006810 GO:0007018 GO:0008017 GO:0015768
8	0.06	0.402	5.68	0.10	0.51	3n94A	GO:0005215 GO:0005363 GO:0006810 GO:0006974 GO:0008643 GO:0015768 GO:0030288 GO:0034289 GO:0042597 GO:0042956 GO:0043190 GO:0055052 GO:0060326 GO:1901982 GO:1990060
9	0.06	0.394	5.87	0.10	0.51	4tsmB	GO:0005215 GO:0005363 GO:0006810 GO:0006974 GO:0008565 GO:0008643 GO:0015627 GO:0015628 GO:0015768 GO:0016020 GO:0016021 GO:0030288 GO:0034289 GO:0042597 GO:0042956 GO:0043190 GO:0055052 GO:0060326 GO:1901982 GO:1990060
10	0.06	0.403	5.67	0.10	0.51	3h4zA	GO:0005215 GO:0005363 GO:0005576 GO:0006810 GO:0006974 GO:0008643 GO:0015768 GO:0030288 GO:0034289 GO:0042597 GO:0042956 GO:0043190 GO:0055052 GO:0060326 GO:1901982 GO:1990060
11	0.06	0.389	5.17	0.08	0.48	1y4cA	GO:0005215 GO:0005363 GO:0006810 GO:0006974 GO:0008643 GO:0015768 GO:0030288 GO:0034289 GO:0042597 GO:0042956 GO:0043190 GO:0055052 GO:0060326 GO:1901982 GO:1990060
12	0.06	0.403	5.47	0.07	0.51	4rwfA	GO:0001525 GO:0001570 GO:0001605 GO:0001635 GO:0001666 GO:0002040 GO:0004871 GO:0004888 GO:0004930 GO:0004948 GO:0005215 GO:0005363 GO:0005737 GO:0005764 GO:0005768 GO:0005783 GO:0005886 GO:0005887 GO:0005905 GO:0006171 GO:0006810 GO:0006816 GO:0006886 GO:0006974 GO:0007165 GO:0007166 GO:0007186 GO:0007187 GO:0007189 GO:0007507 GO:0007565 GO:0008217 GO:0008277 GO:0008284 GO:0008565 GO:0008643 GO:0009986 GO:0010628 GO:0015026 GO:0015031 GO:0015768 GO:0016020 GO:0016021 GO:0030288 GO:0030819 GO:0031623 GO:0032355 GO:0032570 GO:0032870 GO:0034289 GO:0034333 GO:0035924 GO:0042597 GO:0042956 GO:0043116 GO:0043190 GO:0043235 GO:0045766 GO:0045986 GO:0048661 GO:0050728 GO:0055052 GO:0060326 GO:0070830 GO:0070831 GO:0071329 GO:0072659 GO:0097084 GO:1901982 GO:1990060 GO:2000352 GO:2001214
13	0.06	0.391	5.92	0.09	0.51	4qvhA	GO:0000287 GO:0005215 GO:0005363 GO:0005886 GO:0006810 GO:0006974 GO:0008643 GO:0008897 GO:0009239 GO:0009366 GO:0015768 GO:0016740 GO:0016780 GO:0019540 GO:0030288 GO:0034289 GO:0042597 GO:0042956 GO:0043190 GO:0055052 GO:0060326 GO:1901982 GO:1990060
14	0.06	0.398	5.15	0.10	0.49	2xz3A	GO:0005215 GO:0005363 GO:0006810 GO:0015768 GO:0016020 GO:0016021 GO:0019031
15	0.06	0.397	5.28	0.10	0.49	5c7rA	GO:0005215 GO:0005363 GO:0006810 GO:0006974 GO:0008643 GO:0015768 GO:0030288 GO:0034289 GO:0042597 GO:0042956 GO:0043190 GO:0055052 GO:0060326 GO:1901982 GO:1990060
16	0.06	0.395	5.73	0.11	0.51	4ifpA	GO:0000166 GO:0002376 GO:0005215 GO:0005363 GO:0005524 GO:0005622 GO:0005634 GO:0005737 GO:0005829 GO:0006810 GO:0006915 GO:0006919 GO:0006954 GO:0006974 GO:0008643 GO:0008656 GO:0015768 GO:0019899 GO:0019904 GO:0030288 GO:0032495 GO:0034289 GO:0042597 GO:0042742 GO:0042956 GO:0042981 GO:0043190 GO:0045087 GO:0050718 GO:0050727 GO:0051402 GO:0055052 GO:0060326 GO:0061702 GO:0072558 GO:1901982 GO:1990060
17	0.06	0.391	5.00	0.08	0.48	4aq4A	GO:0001407 GO:0005215 GO:0006810 GO:0015794 GO:0030288 GO:0042597
18	0.06	0.385	5.08	0.06	0.47	1eu8A	GO:0005215 GO:0005886 GO:0006810 GO:0008643 GO:0016020

Consensus prediction of GO terms

Molecular Function	GO:0015154	GO:0048030
GO-Score	0.57	0.37
Biological Processes	GO:0015766	GO:0016477	GO:0033554	GO:0070887	GO:0006935	GO:0015774	GO:0034288	GO:0034286
GO-Score	0.57	0.37	0.37	0.37	0.37	0.37	0.37	0.37
Cellular Component	GO:0005887	GO:0043190	GO:0030313
GO-Score	0.37	0.37	0.37

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.