I-TASSER results

I-TASSER results for job id Rv1825

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (292 residues)
MSENRPEPVAAETSAATTARHSQADAGAHDAVRRGRHELPADHPRSKVGPLRRTRLTEIL
RGGRSRLVFGTLAILLCLVLGVAIVTQVRQTDSGDSLETARPADLLVLLDSLRQREATLN
AEVIDLQNTLNALQASGNTDQAALESAQARLAALSILVGAVGATGPGVMITIDDPGPGVA
PEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIG
DPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPVK

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSENRPEPVAAETSAATTARHSQADAGAHDAVRRGRHELPADHPRSKVGPLRRTRLTEILRGGRSRLVFGTLAILLCLVLGVAIVTQVRQTDSGDSLETARPADLLVLLDSLRQREATLNAEVIDLQNTLNALQASGNTDQAALESAQARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPVK
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHCCEEEEEEHCCCCCCCHHHHHHHHHHHHHHCCCEEEHCCCCCEEEEHCCEEEEHCCCEEEHCCEEHCCCEEEEEHCCHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEEEEEEHCCCCCCCCCCCEHCC
Conf.Score	9999998888777667888888888876566566667888788888766545554566677765467899999999999999999999986788766455799999999999999999999999999999999998665578999999999999998787555667599999779999999999999999999889589987877656898676999759979998889679889999959999999985787999999998978999986579995788888876656679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MSENRPEPVAAETSAATTARHSQADAGAHDAVRRGRHELPADHPRSKVGPLRRTRLTEILRGGRSRLVFGTLAILLCLVLGVAIVTQVRQTDSGDSLETARPADLLVLLDSLRQREATLNAEVIDLQNTLNALQASGNTDQAALESAQARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPVK
Prediction	8665665464644445444644465544554555545734554455444444454244434443333010101122133323131100122455553555425403520561454355046325514631551475356555335414541552433232341434203010313665033610230031036130200101444342100020103133320102544130202020104274036105235102410463604141455550403225553526204438
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHCCEEEEEEHCCCCCCCHHHHHHHHHHHHHHCCCEEEHCCCCCEEEEHCCEEEEHCCCEEEHCCEEHCCCEEEEEHCCHHHHHHHHHCCHHHHHHHHHHCCEEEEEEEEEEEEHCCCCCCCCCCCEHCC
MSENRPEPVAAETSAATTARHSQADAGAHDAVRRGRHELPADHPRSKVGPLRRTRLTEILRGGRSRLVFGTLAILLCLVLGVAIVTQVRQTDSGDSLETARPADLLVLLDSLRQREATLNAEVIDLQNTLNALQASGNTDQAALESAQARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPVK

3gmgA

1.00

0.49

1.84

MUSTER

----------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPV-

4tshA

0.07

0.03

0.41

1.06

dPPAS2

------------------------VGTQTGNPATNLPEAQGSASKEAEQSQNQAGETNGSIPVEVPKTDLDQAAKDAKSAGV-------NVVQDADVNKGTVKTAEEAVQKETEIKEDYTKQAEDIKKTTDQYKSDVAAHEAEVAKIKAKNQ--------------------------------------------------------------------------------------------------------------------------------------------

3gmgA

1.00

0.49

2.13

dPPAS

----------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPV-

4tkoB3

0.08

0.03

0.37

1.02

dPPAS2

---------------------------------------------LHISLSAKKLKVESLKKKREALREKLLQVEEKIKLVKLDWERYKSLFQKGLIPRRKFEEVDTNLKVLLHEREYLEKSIQEINTEIKRAKKGIENARNEFKTIEELKK--------------------------------------------------------------------------------------------------------------------------------------------

3gmgA

1.00

0.49

3.61

wdPPAS

----------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPV-

5appA

0.05

0.02

0.41

1.01

dPPAS2

-----------------------------------------KQIEDKIEEILSKIYHIENEIARIKKLIQNVDVRSTENAARSRANEQKIAEKADKADVEKNRADIAANSRAIATFRSSSQNIAALTTKMKQIEDKIEEILSKIYHIENEIARIKKLIKLH-----------------------------------------------------------------------------------------------------------------------------------

3gmgA

1.00

0.49

2.53

wMUSTER

----------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPV-

3gmgA

1.00

0.49

4.04

wPPAS

----------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPV-

3gmgA

1.00

0.49

5.53

dPPAS2

----------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPV-

3gmgA

1.00

0.49

3.21

PPAS

----------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPGVAPEVMIDVINELRAAGAEAIQINDAHRSVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGGAQDGVKRVGGRMVVQQADRVDVTALRQPKQHQYAQPV-

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.24

Estimated TM-score = 0.27±0.08

Estimated RMSD = 16.8±2.9Å

Download Model 2

C-score=-4.30

Download Model 3

C-score=-3.26

Download Model 4

C-score=-4.56

Download Model 5

C-score=-4.65

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3gmgA	0.480	0.96	0.993	0.490
2	3ir7A	0.415	5.88	0.068	0.671
3	4rasA	0.395	6.48	0.067	0.688
4	1ti4K1	0.381	6.78	0.035	0.699
5	2e7zA	0.377	6.15	0.043	0.634
6	1wpxA	0.376	6.04	0.047	0.613
7	2ivfA	0.372	6.51	0.042	0.644
8	5iswA	0.372	6.24	0.031	0.620
9	3qfkA	0.372	5.84	0.041	0.606
10	1ulqC	0.371	6.46	0.053	0.640

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.13	7	3pg9A	TYR	Rep, Mult	168,169,237,238,239,240,241,271,272
2	0.07	4	3fgvB	UNL	Rep, Mult	170,236,267
3	0.06	3	1siwA	SF4	Rep, Mult	192,196,197,238,240,242,245
4	0.04	2	2jirA	NO2	Rep, Mult	215,216,217,227
5	0.04	2	2e7zA	SF4	Rep, Mult	85,185,188,189,191,199,200,236,237
6	0.02	1	3attA	MG	Rep, Mult	189,226
7	0.02	1	1stgA	CO	Rep, Mult	209,226,227
8	0.02	1	3pkyA	MN	Rep, Mult	171,173,267,269
9	0.02	1	1t9yA	MC2	Rep, Mult	200,237,239
10	0.02	1	1yklG	DHB	Rep, Mult	165,166
11	0.02	1	2e75G	OPC	Rep, Mult	126,130
12	0.02	1	1sijA	FES	Rep, Mult	156,157,159,160,161,162,209,211
13	0.02	1	1kj4B	III	Rep, Mult	233,236
14	0.02	1	4pv1G	III	Rep, Mult	126,148

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1ogyI	0.353	6.53	0.034	0.637	1.7.99.4	NA
2	0.060	1w36C	0.353	6.26	0.074	0.599	3.1.11.5	187,223
3	0.060	2zxcA	0.359	6.42	0.028	0.616	3.5.1.23	221
4	0.060	1m1tA	0.358	6.40	0.057	0.610	2.3.1.9	NA
5	0.060	3bxvA	0.344	4.92	0.041	0.476	1.13.11.55	NA
6	0.060	2iikB	0.363	6.60	0.044	0.634	2.3.1.16	NA
7	0.060	1wdkD	0.372	6.44	0.052	0.637	2.3.1.16	NA
8	0.060	1vlbA	0.360	6.35	0.053	0.613	1.2.99.7	NA
9	0.060	1ut9A	0.357	5.39	0.050	0.538	3.2.1.4	NA
10	0.060	2nyaF	0.331	6.53	0.043	0.582	1.7.99.4	50
11	0.060	1n63B	0.347	6.50	0.053	0.596	1.2.99.2	NA
12	0.060	1cpyA	0.336	6.63	0.051	0.606	3.4.16.5	NA
13	0.060	2ivfA	0.372	6.51	0.042	0.644	1.17.99.2	202,203
14	0.060	3hq2B	0.351	5.95	0.041	0.548	3.4.24.-	NA
15	0.060	1ogyA	0.354	6.57	0.034	0.640	1.7.99.4	NA
16	0.060	2z5xA	0.354	5.92	0.061	0.572	1.4.3.4	NA
17	0.060	1o5wC	0.330	6.52	0.083	0.572	1.4.3.4	NA
18	0.060	1q16A	0.318	6.73	0.027	0.582	1.7.99.4	NA
19	0.060	1dgjA	0.356	6.38	0.038	0.610	1.2.-.-	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.53	0.480	0.96	0.99	0.49	3gmgA	GO:0005576 GO:0005886 GO:0005887 GO:0016020 GO:0016021
1	0.06	0.291	6.90	0.06	0.53	1dmrA	GO:0009055 GO:0016491 GO:0030151 GO:0042597 GO:0046872 GO:0050626 GO:0055114
2	0.06	0.293	6.64	0.04	0.52	1fdiA	GO:0005829 GO:0008863 GO:0009055 GO:0015942 GO:0015944 GO:0016491 GO:0030151 GO:0046872 GO:0051536 GO:0051539 GO:0055114
3	0.06	0.368	6.44	0.05	0.63	2v45A	GO:0005506 GO:0006777 GO:0008940 GO:0009055 GO:0016491 GO:0030151 GO:0042128 GO:0042597 GO:0046872 GO:0051536 GO:0051539 GO:0055114
4	0.06	0.308	6.97	0.03	0.57	1tmoA	GO:0009055 GO:0016491 GO:0030151 GO:0042597 GO:0046872 GO:0050626 GO:0055114
5	0.06	0.355	6.45	0.05	0.63	1kqfA	GO:0008430 GO:0008863 GO:0009055 GO:0009061 GO:0009326 GO:0015944 GO:0016020 GO:0016491 GO:0030151 GO:0030288 GO:0036397 GO:0042597 GO:0043546 GO:0045333 GO:0046872 GO:0047111 GO:0051536 GO:0051539 GO:0055114
6	0.06	0.372	6.51	0.04	0.64	2ivfA	GO:0016491 GO:0030151 GO:0046872 GO:0051536 GO:0051539 GO:0055114
7	0.06	0.298	6.14	0.04	0.50	3szyA	GO:0003824 GO:0004551 GO:0008152 GO:0016787 GO:0035529 GO:0046872 GO:0047400
8	0.06	0.409	6.03	0.06	0.68	3egwA	GO:0005886 GO:0008940 GO:0009055 GO:0009061 GO:0009325 GO:0016020 GO:0016491 GO:0030151 GO:0031224 GO:0031235 GO:0042126 GO:0042128 GO:0043546 GO:0044799 GO:0046872 GO:0051536 GO:0051539 GO:0055114
9	0.06	0.286	6.60	0.04	0.51	3ml1A	GO:0005506 GO:0006777 GO:0008940 GO:0009055 GO:0016491 GO:0030151 GO:0042128 GO:0042597 GO:0046872 GO:0051536 GO:0051539 GO:0055114
10	0.06	0.282	6.37	0.04	0.48	2vpwA	GO:0016491 GO:0030151 GO:0046872 GO:0051536 GO:0051539 GO:0055114
11	0.06	0.299	6.45	0.06	0.51	4v4cA	GO:0016491 GO:0018706 GO:0030151 GO:0046872 GO:0055114
12	0.06	0.277	6.58	0.06	0.48	1h0hA	GO:0008863 GO:0009055 GO:0016491 GO:0030151 GO:0042597 GO:0043546 GO:0045333 GO:0046872 GO:0047111 GO:0051536 GO:0051539 GO:0055114
13	0.06	0.377	6.15	0.04	0.63	2e7zA	GO:0008152 GO:0016491 GO:0016829 GO:0018818 GO:0030151 GO:0043546 GO:0046872 GO:0051536 GO:0051539 GO:0055114
14	0.06	0.243	5.09	0.04	0.34	4bivA	GO:0000155 GO:0000160 GO:0000166 GO:0004673 GO:0004871 GO:0005524 GO:0005622 GO:0005886 GO:0007165 GO:0016020 GO:0016021 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0018106 GO:0023014 GO:0043708 GO:0046777
15	0.06	0.256	6.49	0.03	0.44	4etpA	GO:0000166 GO:0000741 GO:0000742 GO:0000743 GO:0003777 GO:0005524 GO:0005634 GO:0005737 GO:0005816 GO:0005856 GO:0005874 GO:0005881 GO:0007018 GO:0007049 GO:0007064 GO:0007067 GO:0007126 GO:0008017 GO:0008569 GO:0046982 GO:0051301 GO:0060236
16	0.06	0.335	6.85	0.06	0.60	5chcA	GO:0016491 GO:0030151 GO:0046872 GO:0051536 GO:0051539 GO:0055114
17	0.06	0.318	6.95	0.09	0.59	1eu1A	GO:0009055 GO:0016491 GO:0030151 GO:0042597 GO:0046872 GO:0050626 GO:0055114
18	0.06	0.264	5.83	0.02	0.42	1f9wA	GO:0000166 GO:0000741 GO:0000742 GO:0000743 GO:0003777 GO:0005524 GO:0005634 GO:0005737 GO:0005816 GO:0005856 GO:0005874 GO:0005881 GO:0007018 GO:0007049 GO:0007064 GO:0007067 GO:0007126 GO:0008017 GO:0008569 GO:0046982 GO:0051301 GO:0060236

Consensus prediction of GO terms

Molecular Function	GO:0046914	GO:0003824
GO-Score	0.47	0.33
Biological Processes	GO:0044710
GO-Score	0.47
Cellular Component	GO:0005576	GO:0005887
GO-Score	0.53	0.53

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.