I-TASSER results

I-TASSER results for job id Rv1823

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (307 residues)
MAESDRLLGGYDPNAGYSAHAGAQPQRIPVPSLLRALLSEHLDAGYAAVAAERERAAAPR
CWQARAVSWMWQALAATLVAAVFAAAVAQARSVAPGVRAAQQLLVASVRSTQAAATTLAQ
RRSTLSAKVDDVRRIVLADDAEGQRLLARLDVLSLAAASAPVVGPGLTVTVTDPGASPNL
SDVSKQRVSGSQQIILDRDLQLVVNSLWESGAEAISIDGVRIGPNVTIRQAGGAILVDNN
PTSSPYTILAVGPPHAMQDVFDRSAGLYRLRLLETSYGVGVSVNVGDGLALPAGATRDVK
FAKQIGP

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MAESDRLLGGYDPNAGYSAHAGAQPQRIPVPSLLRALLSEHLDAGYAAVAAERERAAAPRCWQARAVSWMWQALAATLVAAVFAAAVAQARSVAPGVRAAQQLLVASVRSTQAAATTLAQRRSTLSAKVDDVRRIVLADDAEGQRLLARLDVLSLAAASAPVVGPGLTVTVTDPGASPNLSDVSKQRVSGSQQIILDRDLQLVVNSLWESGAEAISIDGVRIGPNVTIRQAGGAILVDNNPTSSPYTILAVGPPHAMQDVFDRSAGLYRLRLLETSYGVGVSVNVGDGLALPAGATRDVKFAKQIGP
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEHCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCEHCHHHHHHHHHHHHHHCCCEEEHCCEEEHCCEEEEHCCCEEEHCCEEHCCCEEEEEHCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEEEEHCCCCCCCCCCCEHCCC
Conf.Score	9987777788888888888888889888886999999976789875777765555778887766646789999999999999999999997567655788999999999999999999999999999999999986555569999999999999987875557575999995898877777888777888764556999999999999988858998999986786999859989998888579889999869999999986675899999999974978999996689986899888654466799
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MAESDRLLGGYDPNAGYSAHAGAQPQRIPVPSLLRALLSEHLDAGYAAVAAERERAAAPRCWQARAVSWMWQALAATLVAAVFAAAVAQARSVAPGVRAAQQLLVASVRSTQAAATTLAQRRSTLSAKVDDVRRIVLADDAEGQRLLARLDVLSLAAASAPVVGPGLTVTVTDPGASPNLSDVSKQRVSGSQQIILDRDLQLVVNSLWESGAEAISIDGVRIGPNVTIRQAGGAILVDNNPTSSPYTILAVGPPHAMQDVFDRSAGLYRLRLLETSYGVGVSVNVGDGLALPAGATRDVKFAKQIGP
Prediction	7555554244241744456545642544211410440264223532552355466564454344332110111222232231212111224445544454254035205614643550463355146405513752566654355035415414231323405333030103135444546444464443422202250023002102412020010461101020102112320203444231201020114373036005234014303412732503041444650403246345252046358
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEHCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCEHCHHHHHHHHHHHHHHCCCEEEHCCEEEHCCEEEEHCCCEEEHCCEEHCCCEEEEEHCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEEEEHCCCCCCCCCCCEHCCC
MAESDRLLGGYDPNAGYSAHAGAQPQRIPVPSLLRALLSEHLDAGYAAVAAERERAAAPRCWQARAVSWMWQALAATLVAAVFAAAVAQARSVAPGVRAAQQLLVASVRSTQAAATTLAQRRSTLSAKVDDVRRIVLADDAEGQRLLARLDVLSLAAASAPVVGPGLTVTVTDPGASPNLSDVSKQRVSGSQQIILDRDLQLVVNSLWESGAEAISIDGVRIGPNVTIRQAGGAILVDNNPTSSPYTILAVGPPHAMQDVFDRSAGLYRLRLLETSYGVGVSVNVGDGLALPAGATRDVKFAKQIGP

3gmgA

0.30

0.13

0.45

1.36

MUSTER

---------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPG-----------------VAPEVMIDVINELRAAGAEAIQINDVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGG---AQDGVKRVGGRMVVQQADRVDVTALQPKQHQYAQPV--

5cwiA2

0.13

0.05

0.37

1.06

dPPAS2

----------------------------------ADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAAEAVKLACELAQEHPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQKAEEVKERCER---------------------------------------------------------------------------------------------------------------------------------------------------------------

3gmgA

0.30

0.13

0.45

1.62

dPPAS

---------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPG-----------------VAPEVMIDVINELRAAGAEAIQINDVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGG---AQDGVKRVGGRMVVQQADRVDVTALQPKQHQYAQPV--

5cwqA2

0.12

0.05

0.39

1.00

dPPAS2

---------------------------SEEAERASEKAQRVLEEARKVSEEAREQGD---------DEVLALALIAIALAVLALAEVACCRGNKEEAERAYEDARRVEEEARKVKESAEEQGD------SEVKRLAEEAEQLAREARRHVQECRGGWLEHHH-------------------------------------------------------------------------------------------------------------------------------------------------

3gmgA

0.30

0.13

0.45

3.06

wdPPAS

---------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPG-----------------VAPEVMIDVINELRAAGAEAIQINDVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGG---AQDGVKRVGGRMVVQQADRVDVTALQPKQHQYAQPV--

3gmgA

0.30

0.13

0.45

1.90

wMUSTER

---------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPG-----------------VAPEVMIDVINELRAAGAEAIQINDVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGG---AQDGVKRVGGRMVVQQADRVDVTALQPKQHQYAQPV--

3gmgA

0.30

0.13

0.45

3.40

wPPAS

---------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPG-----------------VAPEVMIDVINELRAAGAEAIQINDVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGG---AQDGVKRVGGRMVVQQADRVDVTALQPKQHQYAQPV--

3gmgA

0.30

0.13

0.45

4.33

dPPAS2

---------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPG-----------------VAPEVMIDVINELRAAGAEAIQINDVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGG---AQDGVKRVGGRMVVQQADRVDVTALQPKQHQYAQPV--

3gmgA

0.30

0.13

0.45

2.61

PPAS

---------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPG-----------------VAPEVMIDVINELRAAGAEAIQINDVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGG---AQDGVKRVGGRMVVQQADRVDVTALQPKQHQYAQPV--

3gmgA

0.30

0.13

0.45

3.19

Env-PPAS

---------------------------------------------------------------------------------------------------------------------------------------------------ARLAALSILVGAVGATGPGVMITIDDPGPG-----------------VAPEVMIDVINELRAAGAEAIQINDVRVGVDTWVVGVPGSLTVDTKVLSPPYSILAIGDPPTLAAAMNIPGG---AQDGVKRVGGRMVVQQADRVDVTALRPKQHQYAQPV--

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.46

Estimated TM-score = 0.25±0.07

Estimated RMSD = 17.5±2.6Å

Download Model 2

C-score=-4.68

Download Model 3

C-score=-4.70

Download Model 4

C-score=-4.70

Download Model 5

C-score=-4.68

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	5dgqA	0.453	6.12	0.054	0.733
2	4m5dA	0.431	6.02	0.053	0.691
3	3h7lA	0.425	5.94	0.033	0.674
4	4w8yA1	0.421	5.52	0.057	0.625
5	3x17B	0.421	6.10	0.099	0.691
6	1clcA	0.419	6.02	0.041	0.668
7	4u48A	0.413	5.96	0.073	0.668
8	1urjB1	0.407	5.97	0.053	0.648
9	3ikmD	0.405	5.78	0.048	0.629
10	3gmgA	0.405	1.25	0.281	0.417

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.10	5	3fahA	GOL	Rep, Mult	208,211,212,213,216
2	0.06	3	4oyxA	CA	Rep, Mult	170,171,246,247
3	0.04	2	3mdlA	GOL	Rep, Mult	76,78,79,158,225
4	0.04	2	3fgvB	UNL	Rep, Mult	169,248,282
5	0.02	1	1suiA	CA	Rep, Mult	173,238,239
6	0.02	1	2gjxB	UUU	Rep, Mult	165,167,284
7	0.02	1	5bqdB	MG	Rep, Mult	215,221
8	0.02	1	1yklG	DHB	Rep, Mult	164,165
9	0.02	1	1clcA	CA	Rep, Mult	227,229,235,238
10	0.02	1	2xqtE	CVM	Rep, Mult	46,49
11	0.02	1	4plrA	CA	Rep, Mult	210,211
12	0.02	1	4qvpK	MG	Rep, Mult	203,207
13	0.02	1	1wc6A	MG	Rep, Mult	172,173,244,280
14	0.02	1	1sijA	FES	Rep, Mult	251,252,253,255,256,257,258,284
15	0.02	1	2bf0X	CA	Rep, Mult	155,158
16	0.02	1	4rt4D	III	Rep, Mult	200,201
17	0.02	1	3ucpA	MG	Rep, Mult	222,225,228

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1j38A	0.322	6.63	0.069	0.534	3.4.15.1	NA
2	0.060	1l2aE	0.384	6.61	0.044	0.661	3.2.1.4	NA
3	0.060	1xc6A	0.341	6.69	0.053	0.590	3.2.1.23	238
4	0.060	1b37B	0.393	6.05	0.031	0.645	1.5.3.15,1.5.3.14	NA
5	0.060	1x87A	0.371	6.26	0.053	0.622	4.2.1.49	NA
6	0.060	3gzkA	0.391	5.97	0.116	0.638	3.2.1.4	NA
7	0.060	1prhA	0.383	6.54	0.033	0.632	1.14.99.1	NA
8	0.060	1js4A	0.397	6.19	0.063	0.635	3.2.1.4	NA
9	0.060	1ut9A	0.389	6.77	0.060	0.691	3.2.1.4	NA
10	0.060	1aorB	0.387	6.64	0.069	0.677	1.2.7.5	252
11	0.060	3ikmD	0.405	5.78	0.048	0.629	2.7.7.7	NA
12	0.060	1dl2A	0.369	6.29	0.043	0.632	3.2.1.113	NA
13	0.060	1jqnA	0.368	6.68	0.038	0.635	4.1.1.31	109
14	0.060	2cqsA	0.375	6.18	0.050	0.606	2.4.1.20	NA
15	0.060	1f8rA	0.410	5.68	0.050	0.642	1.4.3.2	NA
16	0.060	2vn4A	0.379	6.03	0.066	0.616	3.2.1.3	NA
17	0.060	2fknB	0.370	6.58	0.051	0.658	4.2.1.49	NA
18	0.060	2vn7A	0.381	6.03	0.067	0.616	3.2.1.3	NA
19	0.060	2rd0A	0.315	4.98	0.043	0.443	2.7.1.153	207,215

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.07	0.425	5.94	0.03	0.67	3h7lA	GO:0003824 GO:0004553 GO:0005975 GO:0008810
1	0.07	0.391	5.97	0.12	0.64	3gzkA	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0046872
2	0.06	0.373	6.16	0.08	0.62	3wc3A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0046872
3	0.06	0.453	6.12	0.05	0.73	5dgqA	GO:0003824 GO:0004553 GO:0005975
4	0.06	0.421	6.07	0.05	0.68	4cj1A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245
5	0.06	0.371	6.19	0.10	0.61	1g87B	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0030246 GO:0030248
6	0.06	0.355	6.18	0.10	0.59	1ia7A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0046872
7	0.06	0.389	6.77	0.06	0.69	1ut9A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016162 GO:0016787 GO:0016798 GO:0030245
8	0.06	0.320	6.39	0.04	0.54	5a29A	GO:0016829 GO:0016837 GO:0042597 GO:0045490
9	0.06	0.291	6.59	0.07	0.50	1j4bA	GO:0000166 GO:0000287 GO:0003924 GO:0004019 GO:0005525 GO:0005737 GO:0006163 GO:0006164 GO:0006167 GO:0006531 GO:0006541 GO:0009168 GO:0014850 GO:0016020 GO:0016874 GO:0035690 GO:0042594 GO:0042803 GO:0044208 GO:0046040 GO:0046872 GO:0051015 GO:0071257
10	0.06	0.296	6.86	0.04	0.52	1ktbA	GO:0003824 GO:0004553 GO:0004557 GO:0005737 GO:0005764 GO:0005975 GO:0008152 GO:0008456 GO:0016139 GO:0016787 GO:0016798
11	0.06	0.431	6.02	0.05	0.69	4m5dA	GO:0000462 GO:0003723 GO:0005634 GO:0005654 GO:0005730 GO:0006364 GO:0006409 GO:0030529 GO:0031167 GO:0032040 GO:0032545 GO:0034456 GO:0042254
12	0.06	0.420	5.98	0.09	0.68	3x17A	GO:0000272 GO:0003824 GO:0004553 GO:0005975 GO:0008152 GO:0008810 GO:0016787 GO:0016798 GO:0030245 GO:0046872
13	0.06	0.346	6.74	0.04	0.61	4ngeD	GO:0003824 GO:0004222 GO:0005739 GO:0005759 GO:0006508 GO:0008047 GO:0008233 GO:0008237 GO:0008270 GO:0016485 GO:0016787 GO:0043085 GO:0046872
14	0.06	0.284	6.94	0.05	0.52	3kjxD	GO:0003677 GO:0003700 GO:0006351 GO:0006355
15	0.06	0.285	6.66	0.06	0.49	4b2tb	GO:0000166 GO:0002199 GO:0005524 GO:0005737 GO:0005832 GO:0005874 GO:0006457 GO:0007339 GO:0031625 GO:0043209 GO:0044297 GO:0051082 GO:0051131 GO:0070062 GO:1901998
16	0.06	0.254	6.60	0.04	0.44	3mxtA	GO:0000166 GO:0004592 GO:0005524 GO:0005737 GO:0015940 GO:0016874
17	0.06	0.243	6.96	0.03	0.43	2g9dA	GO:0006525 GO:0006527 GO:0008152 GO:0008270 GO:0009017 GO:0016787 GO:0016788 GO:0019544 GO:0019545 GO:0046872
18	0.06	0.233	6.86	0.06	0.42	3rq0A	GO:0004553 GO:0005975 GO:0016787 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0016798
GO-Score	0.57
Biological Processes	GO:0030243	GO:0051275
GO-Score	0.36	0.36
Cellular Component
GO-Score

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.