I-TASSER results

I-TASSER results for job id Rv1779c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (597 residues)
MCAHEYAEQRSAVSGIEGLLTWLGGGHWRELGERHERSTHAVAGVIVAVGAALAGLLASL
AVSEAAQGPISSPIGAASLALVLGLLVGAVTRGTASGPARGRAGVTGRASVAVAVGFVVG
ELAALVMFSGAIDRRLDEQAMHSADATPAAVQASASLQQARNARTALDSAVERARGRLDD
ALVVARCEYHPTPACPQTRITGVPGRGPETRTANQLLADAQRELDNALAARDHQAPALDA
KMAHDEQALAEVRQAVVADAGRGLGSRWVAMNDLTLASAGALTARMLAIAFFALLYLLPL
ILRLWRGDTTHDRHAAARAERERAELEADTAIAIKRAEVRRAAEIMWAEHQLTQTRLAIE
AQAEIDREQQRRRVVEALEGPVRASSERTLQPVEDEVYLPIAAETEAASRTVAQLPAGAA
HHRPGIAKNLPAQVQPEGAVEPREKRATPVIRSIPDATKAAARWIRPLVPPFVARMLDNT
TAPLRTARQVFEEVEEIAFSFKRTHKVTVNAEGSDPNDQPPLESHSPAAPAESNPIASSD
SARRSRLATNDDHPPLAQVPPRDLASLSVGSTGELTQREGPHELRSPDGPRQLPPPR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MCAHEYAEQRSAVSGIEGLLTWLGGGHWRELGERHERSTHAVAGVIVAVGAALAGLLASLAVSEAAQGPISSPIGAASLALVLGLLVGAVTRGTASGPARGRAGVTGRASVAVAVGFVVGELAALVMFSGAIDRRLDEQAMHSADATPAAVQASASLQQARNARTALDSAVERARGRLDDALVVARCEYHPTPACPQTRITGVPGRGPETRTANQLLADAQRELDNALAARDHQAPALDAKMAHDEQALAEVRQAVVADAGRGLGSRWVAMNDLTLASAGALTARMLAIAFFALLYLLPLILRLWRGDTTHDRHAAARAERERAELEADTAIAIKRAEVRRAAEIMWAEHQLTQTRLAIEAQAEIDREQQRRRVVEALEGPVRASSERTLQPVEDEVYLPIAAETEAASRTVAQLPAGAAHHRPGIAKNLPAQVQPEGAVEPREKRATPVIRSIPDATKAAARWIRPLVPPFVARMLDNTTAPLRTARQVFEEVEEIAFSFKRTHKVTVNAEGSDPNDQPPLESHSPAAPAESNPIASSDSARRSRLATNDDHPPLAQVPPRDLASLSVGSTGELTQREGPHELRSPDGPRQLPPPR
Prediction	CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	987677888874789998877753898555467777666678889999999999999999998876545478999999999999999986554335777776556889999999999998888898866779999999999999998898888999999999999999999999999999999998876567776667777777787789999999999999999999999999999999999999999999998887756788999999999765676567999999999999999999998647898646899999999999999999888888889999999999999888988888888877899999999876555544455677777667888888877778888888888888888888888888889988888888888888888998888888888889998888888889999888888887777778787777777788888899899999889999988888888889888888999889998888998778889999888888889998889999988899999
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 580 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MCAHEYAEQRSAVSGIEGLLTWLGGGHWRELGERHERSTHAVAGVIVAVGAALAGLLASLAVSEAAQGPISSPIGAASLALVLGLLVGAVTRGTASGPARGRAGVTGRASVAVAVGFVVGELAALVMFSGAIDRRLDEQAMHSADATPAAVQASASLQQARNARTALDSAVERARGRLDDALVVARCEYHPTPACPQTRITGVPGRGPETRTANQLLADAQRELDNALAARDHQAPALDAKMAHDEQALAEVRQAVVADAGRGLGSRWVAMNDLTLASAGALTARMLAIAFFALLYLLPLILRLWRGDTTHDRHAAARAERERAELEADTAIAIKRAEVRRAAEIMWAEHQLTQTRLAIEAQAEIDREQQRRRVVEALEGPVRASSERTLQPVEDEVYLPIAAETEAASRTVAQLPAGAAHHRPGIAKNLPAQVQPEGAVEPREKRATPVIRSIPDATKAAARWIRPLVPPFVARMLDNTTAPLRTARQVFEEVEEIAFSFKRTHKVTVNAEGSDPNDQPPLESHSPAAPAESNPIASSDSARRSRLATNDDHPPLAQVPPRDLASLSVGSTGELTQREGPHELRSPDGPRQLPPPR
Prediction	723451454443042001000000003263045424232000000001210220110001000100001110111010102210110000100011333243210000000000100100110001002640454045423542542443154454045245425404540551254144313204242443452543432112131430540453155045405504552551254045424434452454443434542300020140034014442001000000000010001000001002453324243444444433434443223344342334333442554234442424324414446334413531543344335432533453241434444454445345344544555543264444445455434345644422225214244243322222123234523344543353244324534424332433441324454455553445555435434534354444554464434544444453443553443444545424656415616467245743568
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560                 580

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
MCAHEYAEQRSAVSGIEGLLTWLGGGHWRELGERHERSTHAVAGVIVAVGAALAGLLASLAVSEAAQGPISSPIGAASLALVLGLLVGAVTRGTASGPARGRAGVTGRASVAVAVGFVVGELAALVMFSGAIDRRLDEQAMHSADATPAAVQASASLQQARNARTALDSAVERARGRLDDALVVARCEYHPTPACPQTRITGVPGRGPETRTANQLLADAQRELDNALAARDHQAPALDAKMAHDEQALAEVRQAVVADAGRGLGSRWVAMNDLTLASAGALTARMLAIAFFALLYLLPLILRLWRGDTTHDRHAAARAERERAELEADTAIAIKRAEVRRAAEIMWAEHQLTQTRLAIEAQAEIDREQQRRRVVEALEGPVRASSERTLQPVEDEVYLPIAAETEAASRTVAQLPAGAAHHRPGIAKNLPAQVQPEGAVEPREKRATPVIRSIPDATKAAARWIRPLVPPFVARMLDNTTAPLRTARQVFEEVEEIAFSFKRTHKVTVNAEGSDPNDQPPLESHSPAAPAESNPIASSDSARRSRLATNDDHPPLAQVPPRDLASLSVGSTGELTQREGPHELRSPDGPRQLPPPR

4uxvA

0.09

0.08

0.87

1.05

MUSTER

---KIYAEIDRLESWKIEILNRSI---VEEMSKIKHLK---------MTGQTEEFFEKWREEWDEIVTAHMPKVEELLYDA--------------ENADKYR------------FKKANQVLVHIDDLLTAAESSIEKILREISDLVTSEEKSREEIEQVRERYSKSRKNLLAYSHLYGELYDSLEKDLDEIWYITAR---------KVLLEQDRNLERLQSYIDDVPKLLADCKQTVPGQIAKLKDGYGEMKEKGYKLEHIQLDKELENLSNQLKRAEHVLMTELDIDEASAILQLIDENIQSVYQQLEGEVEAGQSVLSKMPELIIAYDKLKEEKEHTKAETELVKESYRLTAGELGKQQAFEKRLDEIGKLLSSVKDKLDAEHVAYSLLVEEVASIEKQIEEVKKEHAEYRENLQALRKEELQARETLSNLKKTISETARLLKTSNIPGIPSHIQEMLENAHHHIQ--ETVNQLNELPLNMEEAGAHLKQAEDIVNRASRESEELVEQVILIEKIIQFGNRFRSQNHILSEQLKEAERRFYAFDYDDSYEIAAAAVEKAAPGAVEKIKAD------------------------

4uxvA

0.08

0.06

0.82

0.95

dPPAS

---------------------------------------------------------------------KIYAEIDRLESWKIEILNRSIVEEMSKIKHLKMTGQTEEFFEKWREEWDEIVTAHMPKVEELLYDAFKKANQVLVHIDDLLTAAESSIEKILREISDLVTSEEKSREEIEQVRERYSKSRKNLLAYS--------------HLYGELYDSLEKDLDEIWYITARKVLLEQDRNLERLQSYIDDVPKLLADCKQTVPGQIAKLKDGYGEGYKLEHIQLDKELENLSNQLKRAEHVLMTELDIDEASAILQLIDENIQSVYQQLEGEVEAGQSVLSKMPELIIAYDKLKEEKEHYRLQQAFEKRLDEIGKLLSSVKDKLDAEHVAYSLLVEEVASIEKQIEEVKKEHAEYRENLQALRKEELQARETLSNLKKTISETARLLKTSNIPGIPSHIQEMLE-NAHHHIQETVNQLNELPLNMEEAGAHLKQAEDIVNRASRESEELVEQVILIEKIIQFGNRFRSQNHILSEQLKEAERRFYAFDYDDSYEIAAAAVEKAAPGAVEKIKAD---------------------

5j65A

0.06

0.03

0.60

0.83

wdPPAS

-------------HSLIHTIKLNSNKKPGDMTNG---------NQFIISKQEWATIGAYIQTGLGLPVNEQQLRTHVNL----ISIPSDFSQLYDV-------KTSAEWWNKNLYPLIIKSANDIASYGFKVADGYFKKLQDELDNIVDDDAIAKAIKDFKARCGILIKEAKQYEEAAKNIVTSLDQFLHGDQKKLEG-----------VINIQKRLKEVQTALNQAHGESSPAHKELLEKVKNLKTTLERTIKAEVYEILENIKNQIDEIKKQLDSAQLDRDVKIIGMLNSINTDIDNLYSQG------QEAIKVFQKLQGIWATIGAQIENLRTTSLQEVQDSDDADEIQIELEDASDAWLVVAQEARDFTLNAYSTSNLEYKCPENNFMIYWYNNSDWYNNSDW----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

5fvmA

0.10

0.97

1.07

wMUSTER

ELAADKWASSEVKRAIAPLAAGAA-AQWDRVEQKPQSPDKAFFDAILCLHRFEKAAEHIFSARDLLVTEMSALYNRAYGVVVRTQMVAELEEIIQIKPKQDMKVWIKFANLCRK-RLGLAQKTLNTLLEPPVVYAQLKYMWATGSQKEALNHLISFTSRMAHDLQHHIEDYTKLLARCFLKQGEWRVSLQPNWRLENPDAI------GSYLLATHFDKTWYKAWHNWALANFEVTSSLTQRIKDDKVPPTLNANDNFPPELVQVPAIKGFFHSIALSQSSLQDTLRLLTLWFKFPEAAQAMHEGFGLIKILSLLADLGKAHPQALVYPLTVAIKSRQRAALSIIDKMRMHSPKLVNQAELVSDELIRVAWHELWYEGLEDASRQFFGEHNTEKMFATLEPLHEMLKRGPETLREISFQNSFGRDLNYKRTKDINNLNQAWDIYYNVFRRISRKLPQLQTLDLQHVSPKLSAAKDLELAVPGTYHAGKPTHFEPVFTVISSKQRPRRVIIDPESFQRHLNIQQYPAIPLSPKGWVPNSDT-EHREASKIPLNIEHRIMLQMAPDYDNLTLLQKTYALDNTKGQDL------LKSRSSE

4cgkA

0.09

0.04

0.49

1.03

wPPAS

-------------------------------------------------------------------------------------------------------------------------------------------------------------SNLTAQQQEAQKQVDQIQEQVSAIQAEQSNLQ------------------AENDRLQAESKKLEGEITELSKNIVSRNQSLEKQARSAQTNGAVTSYINTIVNSKSIISRVAAMSEIVSANNLEQQKADKKAISEKQVANNDAINTV---IANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLLEQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKVRPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQAAAGFRTGSTPQVGAPTTTSEGF--AD------------------------------------------------------------------------------------------------------------------------------

5cwmA

0.12

0.05

0.38

1.51

dPPAS2

---------------------------------------------------------------------------------------------------------------------------------------DPEDELKRVEKLVKEAEELLRQAKEKGSEEDLEKALRTAEEAAREAKKVLEQAEKEGD--------------PEVALRAVELVVRVAELLLRIAKESGSEEALERALRVAEEAARLAKRVLELAEKQG----------------DPEVALRAVELVVRVAELLLRIAKESGSEEALERALRVAEEAARLAKRVLELAEKQGDPEVARRAVELVKRVAELLERIARESGSEEAKERAERVREEARELQERVKELRER--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

4cgkA

0.09

0.05

0.57

1.19

PPAS

-------------------------------------------------------------------------------------------------------------------------------------------------------------SNLTAQQQEAQKQVDQIQEQVSAIQAEQSNLQ------------------AENDRLQAESKKLEGEITELSKNIVSRNQSLEKQARSAQTNGAVTSYINTIVNSKSIISRVAAMSEIVSAN-NKMLEQQKADKKAISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLLEQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKVRPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIACWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGF--AD----------------------------------------------------------------------------

1vt4I3

0.06

0.05

0.92

1.31

Env-PPAS

--------EYALHRSIVDHYNIPKTFDSDDLIPPYDQYFYSHIGHHLKNIE--------------------HPERMTLFRMVF-LDFRFLEQKIRHDSTAWNAS-----------GSILNTLQQLKFYKPYICDN----DPKYERLVNAILDFLPKIEEICSKYTDLLRIALMAEDEAIFEEAHKQVQRGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG----GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG

4b2nA

0.09

0.98

1.02

MUSTER

LVKQGVNSNPNAALQFNAYFVDLHNPPPPFVNRLPPRPTTCGQFRASATRGRVNLEERQF------FQPMALATSYHFIFLQWDFEEQVSKRYGLYPAPFRNPYPLPGEDPNQTNGGSGQLPLGLIQGKDDNGRWTGLIGASCSACHDSRLGTASE--------ASFKWGLPNSANDAGLLASDMFRTTPITALGNLLPLPWSTGRGSSDAIGLISLLPALFDMETLTLAPSLLEYVADAPHAGMTKAPAWWARAFKTRQFDGSLSSDNVHSEMAFGVANIFRANARRGLEDEFEDINNFLISLSPATYPKTINTALAEQGAVIYHERDLWAGANGAIPKPAGNGSCASCHGVYSPRHAADPNYLPDPRLKGVAAVVTPIETIRTDPRRMRLMADERQRRAWNSGWWAYNNLSPSWTGYPSDNIVASELRRVPRAIYNNGGPIYSPLGPNIWEEPTGYIAPPLYGAWATAPYFHNGSVPNLKPSDRPKLWKRPYTAAGIGGKNAGYDYSFASYDWQKLGWKYTAVACNNSIFTSPFLPCTHNMATIDILYSMWDNVAAQYLNLAYQSPPPITDQQIKSRMVYNSYLYGNDNQSLTDS

5ic0A

0.08

0.06

0.71

0.83

dPPAS

INDMSYFGCLDSVMENSKVLGEAMTGISQNAKNGNLPEFGDAIATASKALCGFTEAAAQAAYLVGVSDPNSQAGQQPTQFARANQAIQMACQSLGEPGCTQAQVLSAATIVAKHTSALCNSCRLASARTANPTAKRQFVQSAKEVANSTANLVKTIKALDGDFTEENRAQCRAATAPLLEAVDNLSAFASNPEFSSVPAQIS-------PEGRAAMEPIVISAKTMLESAGGLIQTARALAVNPRDPPRWSVLAGHSRTVSDSIKKLITSMRDKAPGQLECETAIAALNSCLRDLDQASLAAVSPREGISQEALHTQMLTAVQEISHLIEPLASAARAEASQLGHKVSQMAQYFEPLTLAAVGAASKHPQQMALLDQTKTLAESALQLLYTAKEAGGNPKQAAHTQEALEEAVQMMTEAVEDLTTTLNEAAS---------------------------------------------------------------------------------------------------------------------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 2 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.87

Estimated TM-score = 0.49±0.15

Estimated RMSD = 12.2±4.4Å

Download Model 2

C-score=-0.63

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4uxvA	0.844	1.67	0.088	0.873
2	2pffB	0.342	8.16	0.043	0.571
3	1g8xA	0.311	7.20	0.074	0.472
4	2uvcG	0.311	8.23	0.032	0.524
5	2q7zA	0.305	7.58	0.036	0.486
6	4o9xA	0.300	7.51	0.050	0.469
7	1vw1A	0.298	8.40	0.054	0.516
8	3ripA	0.298	7.06	0.059	0.449
9	4bujB	0.296	7.33	0.032	0.447
10	5d06A	0.292	7.80	0.026	0.469

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.06	3	1hjtA	NO	Rep, Mult	505,509
2	0.04	2	1io4A	NUC	Rep, Mult	108,112,113,115,116,117
3	0.04	2	1qiyL	IPH	Rep, Mult	157,160
4	0.04	2	3cmvG	ANP	Rep, Mult	424,425,426,427,428,429
5	0.04	2	2fkwD	BCL	Rep, Mult	44,47
6	0.04	2	3mylX	POP	Rep, Mult	81,83,84,86,87,114
7	0.02	1	3wmmO	CRT	Rep, Mult	53,70
8	0.02	1	3a52A	MG	Rep, Mult	201,203
9	0.02	1	1xmeC	HAS	Rep, Mult	132,136
10	0.02	1	2vn4A	MAN	Rep, Mult	490,508
11	0.02	1	1gjqB	HEC	Rep, Mult	574,577
12	0.02	1	2qb0B	MN	Rep, Mult	17,44
13	0.02	1	3cmvE	ANP	Rep, Mult	261,262,263,264,306
14	0.02	1	1mneA	POP	Rep, Mult	315,316,317,320,321,322,391,394
15	0.02	1	2j62A	GSZ	Rep, Mult	274,276
16	0.02	1	2d2mD	OXY	Rep, Mult	370,374
17	0.02	1	3pyoY	MG	Rep, Mult	238,283
18	0.02	1	3cmvB	ANP	Rep, Mult	161,163,164,165,166
19	0.02	1	1jb0M	CLA	Rep, Mult	76,80
20	0.02	1	4il6R	HEM	Rep, Mult	248,252

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1y2mB	0.256	6.27	0.046	0.353	4.3.1.24	NA
2	0.060	2np0A	0.219	7.52	0.029	0.343	3.4.24.69	NA
3	0.060	1gaxA	0.247	7.36	0.084	0.387	6.1.1.9	NA
4	0.060	3btaA	0.245	7.18	0.047	0.368	3.4.24.69	NA
5	0.060	1w36C	0.228	7.81	0.022	0.365	3.1.11.5	NA
6	0.060	2pmzQ	0.225	6.76	0.045	0.322	2.7.7.6	102
7	0.060	3hmjA	0.253	8.30	0.042	0.429	2.3.1.86	NA
8	0.060	2vz8B	0.248	7.99	0.043	0.405	2.3.1.85	NA
9	0.060	1mo7A	0.104	6.12	0.036	0.146	3.6.3.9	425
10	0.060	2pffD	0.239	8.29	0.031	0.410	2.3.1.86	NA
11	0.060	2hwgA	0.240	6.46	0.088	0.342	2.7.3.9	NA
12	0.060	3iydD	0.236	7.32	0.027	0.353	2.7.7.6	NA
13	0.060	3c8xA	0.083	5.50	0.063	0.109	2.7.10.1	428
14	0.060	1ug9A	0.247	8.00	0.039	0.412	3.2.1.70	NA
15	0.060	1mhsA	0.228	7.47	0.057	0.357	3.6.3.6	NA
16	0.060	1w27A	0.256	6.65	0.036	0.365	4.3.1.24	NA
17	0.060	2q1fA	0.227	7.52	0.045	0.357	4.2.2.21	NA
18	0.060	3b8eA	0.235	8.02	0.052	0.385	3.6.3.9	NA
19	0.060	2jfdA	0.128	6.16	0.014	0.176	2.3.1.85	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.28	0.844	1.67	0.09	0.87	4uxvA	GO:0000921 GO:0005886 GO:0005940 GO:0007049 GO:0016020 GO:0016021 GO:0051301 GO:0051781
1	0.06	0.273	3.57	0.05	0.31	4uy3A	GO:0000921 GO:0005886 GO:0005940 GO:0007049 GO:0016020 GO:0016021 GO:0051301
2	0.06	0.212	7.80	0.03	0.34	5iy6A	GO:0000398 GO:0000974 GO:0001055 GO:0001172 GO:0003677 GO:0003899 GO:0003968 GO:0005634 GO:0005654 GO:0005665 GO:0005730 GO:0006283 GO:0006351 GO:0006353 GO:0006355 GO:0006366 GO:0006367 GO:0006368 GO:0006370 GO:0008543 GO:0010467 GO:0016740 GO:0016779 GO:0031047 GO:0031625 GO:0033120 GO:0035019 GO:0042795 GO:0044822 GO:0046872 GO:0050434
3	0.06	0.199	8.10	0.02	0.33	5flmA	GO:0001047 GO:0001055 GO:0003677 GO:0003899 GO:0005665 GO:0005719 GO:0006351 GO:0006366 GO:0016740 GO:0016779
4	0.06	0.173	8.65	0.04	0.31	4mz0A	GO:0003824 GO:0008152 GO:0016740 GO:0031177 GO:0046872
5	0.06	0.165	8.16	0.02	0.27	4mz0B	GO:0003824 GO:0008152 GO:0016740 GO:0031177 GO:0046872
6	0.06	0.192	8.15	0.02	0.32	2pffB	GO:0003824 GO:0004312 GO:0004313 GO:0004314 GO:0004318 GO:0004319 GO:0004320 GO:0004321 GO:0005811 GO:0005829 GO:0005835 GO:0006629 GO:0006631 GO:0006633 GO:0008152 GO:0016295 GO:0016296 GO:0016297 GO:0016409 GO:0016491 GO:0016740 GO:0016787 GO:0016829 GO:0019171 GO:0042759 GO:0047451 GO:0055114
7	0.06	0.161	4.54	0.03	0.19	2ch7A	GO:0004871 GO:0005886 GO:0006935 GO:0007165 GO:0016020 GO:0016021
8	0.06	0.160	6.86	0.05	0.24	4yrdB	GO:0003824 GO:0050662
9	0.06	0.130	6.20	0.05	0.18	2cuyA	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746
10	0.06	0.149	6.70	0.02	0.22	3te6A	GO:0000781 GO:0000784 GO:0001308 GO:0003677 GO:0003682 GO:0003690 GO:0003697 GO:0005634 GO:0005677 GO:0005720 GO:0005724 GO:0005730 GO:0006351 GO:0006355 GO:0030466 GO:0031491 GO:0031493 GO:0031507 GO:0042802 GO:0070481
11	0.06	0.142	6.42	0.05	0.20	2c2nA	GO:0003824 GO:0004314 GO:0005739 GO:0006629 GO:0006631 GO:0006633 GO:0008152 GO:0016740 GO:0044822
12	0.06	0.128	6.01	0.02	0.18	3ezoA	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746
13	0.06	0.135	5.77	0.03	0.18	3qatA	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746
14	0.06	0.168	7.04	0.04	0.25	3ig8A	GO:0000166 GO:0004357 GO:0005524 GO:0005622 GO:0005737 GO:0006750 GO:0016874 GO:0017109 GO:0042542 GO:0046686
15	0.06	0.127	3.21	0.05	0.14	1fxkC	GO:0005737 GO:0006457 GO:0016272 GO:0051082
16	0.06	0.129	5.81	0.03	0.17	3ptwA	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746
17	0.06	0.128	6.09	0.03	0.18	2h1yB	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746
18	0.06	0.133	6.13	0.01	0.18	3im8A	GO:0003824 GO:0004314 GO:0008152 GO:0016740 GO:0016746

Consensus prediction of GO terms

Molecular Function	GO:0001055	GO:0046872	GO:0044822	GO:0003968	GO:0031625	GO:0031177	GO:0001047
GO-Score	0.12	0.12	0.06	0.06	0.06	0.06	0.06
Biological Processes	GO:0051302	GO:0048522	GO:0000921	GO:0007049
GO-Score	0.55	0.55	0.32	0.32
Cellular Component	GO:0005886	GO:0005940	GO:0016021
GO-Score	0.32	0.32	0.32

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.