I-TASSER results

I-TASSER results for job id Rv1769

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (414 residues)
MHEVAAREQRSDGPMRLDAQGRLQRYEEAFADYDAPFAFVDLDAMWGNADQLLARAGDKP
IRVASKSLRCRPLQREILDASERFDGLLTFTLTETLWLAGQGFSNLLLAYPPTDRAALRA
LGELTAKDPDGAPIVMVDSVEHLDLIERTTDKPVRLCLDFDAGYWRAGGRIKIGSKRSPL
HTPEQARALAVEIARRPALTLAALMCYEAHIAGLGDNVAGKRVHNAIIRRMQRMSFEELR
ERRARAVELVREVADIKIVNAGGTGDLQLVAQEPLITEATAGSGFYAPTLFDSYSTFTLQ
PAAMFALPVCRRPGAKTVTALGGGYLASGVGAKDRMPTPYLPVGLKLNALEGTGEVQTPL
SGDAARRLKLGDKVYFRHTKAGELCERFDHLHLVRGAEVVDTVPTYRGEGRTFL

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MHEVAAREQRSDGPMRLDAQGRLQRYEEAFADYDAPFAFVDLDAMWGNADQLLARAGDKPIRVASKSLRCRPLQREILDASERFDGLLTFTLTETLWLAGQGFSNLLLAYPPTDRAALRALGELTAKDPDGAPIVMVDSVEHLDLIERTTDKPVRLCLDFDAGYWRAGGRIKIGSKRSPLHTPEQARALAVEIARRPALTLAALMCYEAHIAGLGDNVAGKRVHNAIIRRMQRMSFEELRERRARAVELVREVADIKIVNAGGTGDLQLVAQEPLITEATAGSGFYAPTLFDSYSTFTLQPAAMFALPVCRRPGAKTVTALGGGYLASGVGAKDRMPTPYLPVGLKLNALEGTGEVQTPLSGDAARRLKLGDKVYFRHTKAGELCERFDHLHLVRGAEVVDTVPTYRGEGRTFL
Prediction	CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHCHHHHHHHHHHHHHHHCCCCEEEEHCCCCHHHHHHHHHHHCCCCEEEEHCCHHHHHHHHHHCCCEEEEHCCCCCHHHHHHHHHHHHHCCCCEEEEEHCCHHHHHHHHHHHCCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHCCCCCEEEEHCCCCCCEEHCCEEEHCCCHHHHHCCCCCCCEEEEEEEEEHCCCCCEEEEHCCCCCCCCCCCCCCCCEEEHCCCCEHCEEEHCCCEEEEEHCCCCCCCCCCEEEEEHCCCHHHHHHHCCEEEEEHCCEEEEEEEEEEHCCCCCC
Conf.Score	998888877788998887556788875777568998788759999999999999968996899984787599999999989986679986999999999989996999678889999999999998778858999978999999999997998799999869999878877777767789999999999999998999699999875755578888656788999999999999999999999999999998999877886789846776558887477557588678456554578887637899887754689989996888655678888888857757986554456566657888678888899988999984777789987898999989989999999875576679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MHEVAAREQRSDGPMRLDAQGRLQRYEEAFADYDAPFAFVDLDAMWGNADQLLARAGDKPIRVASKSLRCRPLQREILDASERFDGLLTFTLTETLWLAGQGFSNLLLAYPPTDRAALRALGELTAKDPDGAPIVMVDSVEHLDLIERTTDKPVRLCLDFDAGYWRAGGRIKIGSKRSPLHTPEQARALAVEIARRPALTLAALMCYEAHIAGLGDNVAGKRVHNAIIRRMQRMSFEELRERRARAVELVREVADIKIVNAGGTGDLQLVAQEPLITEATAGSGFYAPTLFDSYSTFTLQPAAMFALPVCRRPGAKTVTALGGGYLASGVGAKDRMPTPYLPVGLKLNALEGTGEVQTPLSGDAARRLKLGDKVYFRHTKAGELCERFDHLHLVRGAEVVDTVPTYRGEGRTFL
Prediction	655254444436412435453315204410550300000002410440052026206713000000001013002200633431100000003001100524030000000002351032024003514401000000114004301641733030000010022000131432342240332620130042037252020000000000013144444344123301310232024003401520340274304020000000000111372410000000000000020010120211000000000023244310000000100022323421010111321311202011120011314655404234232511000000000131000026440112020010421117
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHCHHHHHHHHHHHHHHHCCCCEEEEHCCCCHHHHHHHHHHHCCCCEEEEHCCHHHHHHHHHHCCCEEEEHCCCCCHHHHHHHHHHHHHCCCCEEEEEHCCHHHHHHHHHHHCCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHCCCCCEEEEHCCCCCCEEHCCEEEHCCCHHHHHCCCCCCCEEEEEEEEEHCCCCCEEEEHCCCCCCCCCCCCCCCCEEEHCCCCEHCEEEHCCCEEEEEHCCCCCCCCCCEEEEEHCCCHHHHHHHCCEEEEEHCCEEEEEEEEEEHCCCCCC
MHEVAAREQRSDGPMRLDAQGRLQRYEEAFADYDAPFAFVDLDAMWGNADQLLARAGDKPIRVASKSLRCRPLQREILDASERFDGLLTFTLTETLWLAGQGFSNLLLAYPPTDRAALRALGELTAKDPDGAPIVMVDSVEHLDLIERTTDKPVRLCLDFDAGYWRAGGRIKIGSKRSPLHTPEQARALAVEIARRPALTLAALMCYEAHIAGLGDNVAGKRVHNAIIRRMQRMSFEELRERRARAVELVREVADIKIVNAGGTGDLQLVAQEPLITEATAGSGFYAPTLFDSYSTFTLQPAAMFALPVCRRPGAKTVTALGGGYLASGVGAKDRMPTPYLPVGLKLNALEGTGEVQTPLSGDAARRLKLGDKVYFRHTKAGELCERFDHLHLVRGAEVVDTVPTYRGEGRTFL

4v15A

0.19

0.17

0.88

2.41

MUSTER

-------RGIALPPAAQP--------GDPLARVDTPSLVLDLPAFEANLRAMQAWADEVALRPHAKAHKCPEIALRQLALGAR--GICCQKVSEALPFVAAGIRDIHISNEVVGPAKLALLGQLARAA---KISVCVDNLAQLSAAMTRAGAEIDVLVEVDVGQGRCG-----------VSDDATVLALAQQARALPGLNFAGLQAYHGSVQHYRT-----------REERAAVCRQAARIAASYAQLLRESGIACDTITGGGTGSVEFDAASGVYTELQAGSYAFMDSDYGANEWLKFQNSLFVLSTVMSTPAPGRVILDAGLKSTTAECGP---PAVYGEPG---LTYAAINDEHGVVRVEPAQAPALGAVLRLVPSHVDPTFNLHDGLVVVKDGVVQDVWEI-AARGFSR-

4v15A

0.19

0.17

0.88

3.02

dPPAS

-------RGIALPPAAQP--------GDPLARVDTPSLVLDLPAFEANLRAMQAWADEVALRPHAKAHKCPEIALRQLALGAR--GICCQKVSEALPFVAAGIRDIHISNEVVGPAKLALLGQLARA---AKISVCVDNLAQLSAAMTRAGAEIDVLVEVDVGQGRCG-----------VSDDATVLALAQQARALPGLNFAGLQAYHGSVQHYRT-----------REERAAVCRQAARIAASYAQLLRESGIACDTITGGGTGSVEFDAASGVYTELQAGSYAFMDSDYGANGPLKFQNSLFVLSTVMSTPAPGRVILDAGLKSTTAECGPP------AVYGEPGLTYAAINDEHGVVRVEPGAQPALGAVLRLVPSHVDPTFNLHDGLVVVKDGVVQDVWEAARGFSR---

4v15A

0.19

0.17

0.88

3.78

wdPPAS

---------------RGIALPPAAQPGDPLARVDTPSLVLDLPAFEANLRAMQAWADEVALRPHAKAHKCPEIALRQLALGAR--GICCQKVSEALPFVAAGIRDIHISNEVVGPAKLALLGQLARAA---KISVCVDNLAQLSAAMTRAGAEIDVLVEVDVGQGRCGV-----------SDDATVLALAQQARALPGLNFAGLQAYHGSVQHYRT-----------REERAAVCRQAARIAASYAQLLRESGIACDTITGGGTGSVEFDAASGVYTELQAGSYAFMDSDYGANEPLKFQNSLFVLSTVMSTPAPGRVILDAGLKSTTAECGPP------AVYGEPGLTYAAINDEHGVVRVEPGAQPALGAVLRLVPSHVDPTFNLHDGLVVVKDGVVQDVWEAARGFSR---

4v15A

0.19

0.17

0.87

3.32

wMUSTER

IALPPAAQP-----------------GDPLARVDTPSLVLDLPAFEANLRAMQAWADEVALRPHAKAHKCPEIALRQLALGAR--GICCQKVSEALPFVAAGIRDIHISNEVVGPAKLALLGQLARAA---KISVCVDNLAQLSAAMTRAGAEIDVLVEVDVGQGRCG-----------VSDDATVLALAQQARALPGLNFAGLQAYHGSVQHYRT-----------REERAAVCRQAARIAASYAQLLRESGIACDTITGGGTGSVEFDAASGVYTELQAGSYAFMDSDYGANEWLKFQNSLFVLSTVMSTPAPGRVILDAGLKSTTAECGP---PAVYGEPG---LTYAAINDEHGVVRVEPAQAPALGAVLRLVPSHVDPTFNLHDGLVVVKDGVVQDVWEI-AARGFS--

4v15A

0.20

0.17

0.87

4.39

wPPAS

--------RGIALPPA-------AQPGDPLARVDTPSLVLDLPAFEANLRAMQAWADEVALRPHAKAHKCPEIALRQLALGAR--GICCQKVSEALPFVAAGIRDIHISNEVVGPAKLALLGQLARAA---KISVCVDNLAQLSAAMTRAGAEIDVLVEVDVGQGRCG-----------VSDDATVLALAQQARALPGLNFAGLQAYHGSVQHYRT-----------REERAAVCRQAARIAASYAQLLRESGIACDTITGGGTGSVEFDAASGVYTELQAGSYAFMDSDYGANEPLKFQNSLFVLSTVMSTPAPGRVILDAGLKSTTAECG---PPAVYGEPG----TYAAINDEHGVVRVEPGAQPALGAVLRLVPSHVDPTFNLHDGLVVVKDGVVQDVWEAARGFSR---

4v15A

0.19

0.17

0.88

5.77

dPPAS2

-------RGIALPPAAQP--------GDPLARVDTPSLVLDLPAFEANLRAMQAWADEVALRPHAKAHKCPEIALRQLALGAR--GICCQKVSEALPFVAAGIRDIHISNEVVGPAKLALLGQLARA---AKISVCVDNLAQLSAAMTRAGAEIDVLVEVDVGQGRCG-----------VSDDATVLALAQQARALPGLNFAGLQAYHGSVQHYRT-----------REERAAVCRQAARIAASYAQLLRESGIACDTITGGGTGSVEFDAASGVYTELQAGSYAFMDSDYGANGPLKFQNSLFVLSTVMSTPAPGRVILDAGLKSTTAECGPP------AVYGEPGLTYAAINDEHGVVRVEPGAQPALGAVLRLVPSHVDPTFNLHDGLVVVKDGVVQDVWEAARGFSR---

4v15A

0.20

0.17

0.88

4.04

PPAS

--------RGIALPPAA-------QPGDPLARVDTPSLVLDLPAFEANLRAMQAWADEVALRPHAKAHKCPEIALRQLALGAR--GICCQKVSEALPFVAAGIRDIHISNEVVGPAKLALLGQLARAA---KISVCVDNLAQLSAAMTRAGAEIDVLVEVDVGQGRCG-----------VSDDATVLALAQQARALPGLNFAGLQAYHGSVQHYRT-----------REERAAVCRQAARIAASYAQLLRESGIACDTITGGGTGSVEFDAASGVYTELQAGSYAFMDSDYGANEWLKFQNSLFVLSTVMSTPAPGRVILDAGLKSTTAECGP---PAVYGEPG---LTYAAINDEHGVVRVEPGAQPALGAVLRLVPSHVDPTFNLHDGLVVVKDGVVQDVWEAARGFSR---

4v15A

0.20

0.18

0.88

4.92

Env-PPAS

--------RGIALPPAA-------QPGDPLARVDTPSLVLDLPAFEANLRAMQAWADEVALRPHAKAHKCPEIALRQLALGAR--GICCQKVSEALPFVAAGIRDIHISNEVVGPAKLALLGQLARAA---KISVCVDNAENLAQLSAAMTAEIDVLVEVDVGQGRCG-----------VSDDATVLALAQQARALPGLNFAGLQAYHGSVQHYRT-----------REERAAVCRQAARIAASYAQLLRESGIACDTITGGGTGSVEFDAASGVYTELQAGSYAFMDSDYGANEPLKFQNSLFVLSTVMSTPAPGRVILDAGLKSTTAECG---PPAVYGEPG---LTYAAINDEHGVVRVEPAQAPALGAVLRLVPSHVDPTFNLHDGLVVVKDGVVQDVWEAARGFSR---

3anuA

0.19

0.17

0.86

2.28

MUSTER

-----------------------MWLGALLDTLPTPALTIDRTTARRNAERMRERCRGVRLRPHVKTHKTLEGGLLATGGTR--RGIAVSTLAEARFFADGGFDDILLAYPVPT-ARLEECAGLARRLD--AFHVLLDRPEALASLRQGHGKRWLVWLKLDCG--RAGVRP----------TDPAALELAQAIANDEEVTLVGVYAHCGNTYCSG------------ADTIQAIARTTTNAVLSFVAALRQAGVPCPQASIGSTPSCSHPIPESQLTELHPGNYIFYDLQQTQLGSCQPDVAIRVLTRVIGHAHRGQLLVDCGWAALSLHGAGGP-QGCAAIDGHPELRLVGLTQEHGLLEHMDFGRFPVGSVLALIPYHACATAAMHPVYYVHEEGKVVALWHPVRGW-----

3wqcA

0.16

0.14

0.86

2.83

dPPAS

--------------------------QDTLLTLDTPAAVIDLDRMQRNIARMQQRMDGVRLRPHVKTSKSVPVAAAQRAAGAS--GITVSTLKEAEQFFAAGTTDILYAVSMA-PHRLPQALQLRRR--GCDLKLIVDSAQAIAAFGREQGEAFEVWIEIDTDGHRSGVGADD----------TPLLLAIGRTLHDGGMRLGGVLTHAGSSYELDT-----------PEALQALAERERAGCVQAAEALRAAGLPCPVVSVGSTPTALAASRLDGVTEVRAGVYVFFDLVMRNIGVCAEDVALSVLATVIGHADKGWAIVDAGWMAMSRDRGTARQKQVCDLQGRVMPVLTGANQEHGILARADGAAFPLGTRLRILPNHACATGAQFPAYQALAADGSVQTWERLHGW-----

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.62

Estimated TM-score = 0.80±0.09

Estimated RMSD = 5.6±3.5Å

Download Model 2

C-score=-1.55

Download Model 3

C-score=-1.17

Download Model 4

C-score=-0.90

Download Model 5

C-score=0.19

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4v15A	0.854	1.53	0.176	0.877
2	3wqcA	0.808	2.11	0.154	0.862
3	3anuA	0.804	2.11	0.169	0.858
4	3llxA	0.773	2.35	0.140	0.838
5	3gwqA	0.735	3.55	0.182	0.858
6	4kbxA	0.693	3.39	0.107	0.804
7	1twiA	0.689	3.57	0.114	0.802
8	3c5qA	0.683	3.86	0.113	0.809
9	3vabA	0.682	3.55	0.122	0.797
10	3n2oA	0.680	3.99	0.084	0.826

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.63	66	2j66A	PLP	Rep, Mult	64,66,88,109,159,205,207,263,280,281,282,283,382
2	0.02	2	3wqeA	MG	Rep, Mult	66,380,382
3	0.01	1	3wqdA	999	Rep, Mult	66,166,207,213,380
4	0.01	1	4pb4A	MG	Rep, Mult	66,382

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.450	2odoA	0.647	3.33	0.150	0.756	5.1.1.1	166
2	0.417	2dy3D	0.644	3.52	0.140	0.761	5.1.1.1	66,166
3	0.307	1epvA	0.653	3.96	0.139	0.795	5.1.1.1	166
4	0.296	1xfcA	0.640	3.80	0.148	0.766	5.1.1.1	166
5	0.183	1tufA	0.689	3.57	0.114	0.802	4.1.1.20	66
6	0.107	2qghA	0.683	3.87	0.113	0.809	4.1.1.20	NA
7	0.106	2pljA	0.657	3.81	0.151	0.780	4.1.1.17	66
8	0.106	3c5qA	0.683	3.86	0.113	0.809	4.1.1.20	164,262
9	0.084	1f3tB	0.655	3.77	0.126	0.775	4.1.1.17	66
10	0.073	1vfsA	0.679	3.39	0.155	0.790	5.1.1.1	66,166
11	0.065	2rjgA	0.642	3.56	0.162	0.758	5.1.1.1	66,166
12	0.063	2vd8A	0.674	3.83	0.155	0.812	5.1.1.1	166
13	0.060	2p3eB	0.676	3.58	0.117	0.787	4.1.1.20	66
14	0.060	3n2bA	0.657	3.95	0.101	0.785	4.1.1.20	64,66,214,262,282
15	0.060	2yxxA	0.644	3.67	0.120	0.758	4.1.1.20	66
16	0.060	3hz3A	0.487	5.52	0.048	0.676	2.4.1.5	NA
17	0.060	3gwqA	0.735	3.55	0.182	0.858	4.3.1.18	51,78
18	0.060	2j66A	0.663	3.49	0.141	0.775	4.1.1.20	NA
19	0.060	1knwA	0.653	4.08	0.109	0.790	4.1.1.20	66
20	0.060	1hkvA	0.681	3.72	0.136	0.804	4.1.1.20	NA
21	0.060	1xc6A	0.481	5.34	0.040	0.657	3.2.1.23	131
22	0.060	1rcqA	0.646	3.36	0.128	0.756	5.1.1.1	NA
23	0.060	2nv9B	0.643	3.72	0.120	0.766	4.1.1.19	66
24	0.060	3bg3A	0.456	5.58	0.061	0.638	6.4.1.1	NA
25	0.060	3e5pB	0.645	3.71	0.129	0.770	5.1.1.1	166
26	0.060	3hurA	0.576	4.85	0.119	0.751	5.1.1.1	166
27	0.060	2epoA	0.466	5.61	0.080	0.650	3.2.1.52	NA
28	0.060	3cuzA	0.453	5.72	0.072	0.640	2.3.3.9	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.35	0.820	2.36	0.15	0.88	4pb3B	GO:0016829 GO:0016841 GO:0030170
1	0.26	0.756	3.56	0.18	0.88	3gwqA	GO:0008721 GO:0016829
2	0.20	0.669	3.79	0.15	0.81	3ha1A	GO:0003824 GO:0006522 GO:0008784 GO:0016853 GO:0030170 GO:0030632
3	0.19	0.645	3.77	0.11	0.78	4eclA	GO:0003824 GO:0006522 GO:0008784 GO:0016020 GO:0016021 GO:0016747 GO:0016853 GO:0016855 GO:0030170 GO:0046677
4	0.16	0.644	3.52	0.14	0.76	2dy3D	GO:0003824 GO:0006522 GO:0008784 GO:0016853 GO:0030170 GO:0030632
5	0.06	0.356	6.30	0.08	0.55	5fc1A	GO:0004767 GO:0005576 GO:0005615 GO:0006685 GO:0008081 GO:0008270 GO:0009143 GO:0016787 GO:0046872 GO:0070062
6	0.06	0.288	7.27	0.06	0.50	2oblA	GO:0000166 GO:0005524 GO:0005737 GO:0006754 GO:0009058 GO:0016887 GO:0030254 GO:0030257 GO:0044780 GO:0046872 GO:0046961 GO:1902600
7	0.06	0.296	6.54	0.07	0.45	2bkwA	GO:0003824 GO:0006545 GO:0008453 GO:0008483 GO:0016740 GO:0019265
8	0.06	0.271	7.10	0.04	0.45	5bufA	GO:0003824 GO:0003866 GO:0004665 GO:0005737 GO:0006571 GO:0008652 GO:0008977 GO:0009073 GO:0009423 GO:0016491 GO:0016740 GO:0016765 GO:0055114
9	0.06	0.215	6.84	0.05	0.36	5hgcA	GO:0005615
10	0.06	0.233	6.90	0.06	0.38	3dyjA	GO:0001726 GO:0003779 GO:0005178 GO:0005200 GO:0005737 GO:0005815 GO:0005856 GO:0005886 GO:0005925 GO:0007016 GO:0007044 GO:0007155 GO:0009986 GO:0016020 GO:0017166 GO:0030054 GO:0030274 GO:0030866 GO:0032403 GO:0032587 GO:0042995 GO:0051015 GO:0070062 GO:0070527
11	0.06	0.205	6.07	0.08	0.31	4knpA	GO:0003824 GO:0008152
12	0.06	0.217	6.01	0.07	0.32	3qkaD	GO:0003824 GO:0008152
13	0.06	0.212	6.03	0.04	0.32	1yyfC	GO:0004298 GO:0005737 GO:0005839 GO:0006508 GO:0008233 GO:0008236 GO:0009376 GO:0016787 GO:0030163 GO:0046872 GO:0051603
14	0.06	0.202	6.10	0.05	0.31	3ty6A	GO:0003824 GO:0004298 GO:0005737 GO:0005839 GO:0006508 GO:0008152 GO:0008233 GO:0009376 GO:0016787 GO:0030163 GO:0046872 GO:0051603
15	0.06	0.180	4.60	0.08	0.23	3rfqC
16	0.06	0.215	5.58	0.05	0.31	3p2tA	GO:0002250 GO:0002376 GO:0003823 GO:0004872 GO:0005886 GO:0007165 GO:0016020 GO:0016021 GO:0045671 GO:0070062 GO:1902894
17	0.06	0.156	4.80	0.04	0.21	1nogA
18	0.06	0.124	4.00	0.02	0.15	2wscK	GO:0009507 GO:0009522 GO:0009535 GO:0009536 GO:0009579 GO:0015979 GO:0016020 GO:0016021 GO:0016168

Consensus prediction of GO terms

Molecular Function	GO:0030170	GO:0008784	GO:0016746	GO:0016841
GO-Score	0.65	0.46	0.37	0.35
Biological Processes	GO:0042221	GO:0030632
GO-Score	0.37	0.33
Cellular Component	GO:0016021
GO-Score	0.19

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.