I-TASSER results

I-TASSER results for job id Rv1754c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (563 residues)
MYRYQVRVQQRRSEMNRWVATRSRRHTYQWITDHKSPRDHYRHISELRTSIATSSPGRCD
MSPIPRIVSVSLAWAAAIGLMVPIGLAPPAMAAPCSGDAANAPPPPSAIVTDPGATALGP
VRPGHGPIPTGRKPRGANDRAPLPKLGPLISALLNPGARNAAPLQQQALVPRANPGPNPA
PNPPATGPQPPNATQLTPNPAPAPDPAPAAAPDPGATLAGATTSLAEWVTGPDSPNKTLE
RFGISGTDLGIPWDNGDPANRQVLMIFGDTFGYCAVDGHQWRYNTLFRSQDRDLGNGVHV
TSGDASNRYSGSPVRQPGFSKQLINSIKWARDETGIIPTAGIAVGKTQYVNFMSIRNWGR
DGEWTTNYSGIAVSKDNGQTWGVFPGTIRASGPDSGGKARFVPGNENFQMGAYLKSNDGY
LYSFGTPPGRGGSAYLARVPQRFVPDLTKYQYWNGDSNSWVPNKPDAATPVIPGPVGEMS
VQYNTYLKQYLALYTNGMNDVVARTAPAPQGPWSAEQMLVSSWQMPGGIYAPMMHPWSTG
KDVYFNLSLWSAYNVMLMHTVLP

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MYRYQVRVQQRRSEMNRWVATRSRRHTYQWITDHKSPRDHYRHISELRTSIATSSPGRCDMSPIPRIVSVSLAWAAAIGLMVPIGLAPPAMAAPCSGDAANAPPPPSAIVTDPGATALGPVRPGHGPIPTGRKPRGANDRAPLPKLGPLISALLNPGARNAAPLQQQALVPRANPGPNPAPNPPATGPQPPNATQLTPNPAPAPDPAPAAAPDPGATLAGATTSLAEWVTGPDSPNKTLERFGISGTDLGIPWDNGDPANRQVLMIFGDTFGYCAVDGHQWRYNTLFRSQDRDLGNGVHVTSGDASNRYSGSPVRQPGFSKQLINSIKWARDETGIIPTAGIAVGKTQYVNFMSIRNWGRDGEWTTNYSGIAVSKDNGQTWGVFPGTIRASGPDSGGKARFVPGNENFQMGAYLKSNDGYLYSFGTPPGRGGSAYLARVPQRFVPDLTKYQYWNGDSNSWVPNKPDAATPVIPGPVGEMSVQYNTYLKQYLALYTNGMNDVVARTAPAPQGPWSAEQMLVSSWQMPGGIYAPMMHPWSTGKDVYFNLSLWSAYNVMLMHTVLP
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHCCCCCCCCCEEEEEEHCCCCCCCCCCCCCCCCEEEEHCCCCCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHCCCEEEHCCEEEEEEEEHCCCCCCCCEEEEEEEEEEHCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEHCCCCCEEEEEHCCCCCCCCEEEEEHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCCEEEEEEHCCCCCEEEEHCCCCCCCCCCCEEEHCCCCCCCCCCCCCHCCCCCCCEEEEEEEHCCCCCEEEEEEHCC
Conf.Score	98765544456777655566776555555555688887765678888888889999999989998888888888888888888888998889999999999999999999999888899999999999999999999999998889998888889998899999888889999999999999999999889988889999989999999888888888888877766778888898876666876555555445777899879999957899999888887774788757888778777668888876666778889876555567788986578854657998998999999975789997567667899998689988666688767888888887766678888765544468998799996888999977999858888788544678888899888888888876557887578999957788799999779985999986999999988857876888887777765478878985899999758985899998689
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MYRYQVRVQQRRSEMNRWVATRSRRHTYQWITDHKSPRDHYRHISELRTSIATSSPGRCDMSPIPRIVSVSLAWAAAIGLMVPIGLAPPAMAAPCSGDAANAPPPPSAIVTDPGATALGPVRPGHGPIPTGRKPRGANDRAPLPKLGPLISALLNPGARNAAPLQQQALVPRANPGPNPAPNPPATGPQPPNATQLTPNPAPAPDPAPAAAPDPGATLAGATTSLAEWVTGPDSPNKTLERFGISGTDLGIPWDNGDPANRQVLMIFGDTFGYCAVDGHQWRYNTLFRSQDRDLGNGVHVTSGDASNRYSGSPVRQPGFSKQLINSIKWARDETGIIPTAGIAVGKTQYVNFMSIRNWGRDGEWTTNYSGIAVSKDNGQTWGVFPGTIRASGPDSGGKARFVPGNENFQMGAYLKSNDGYLYSFGTPPGRGGSAYLARVPQRFVPDLTKYQYWNGDSNSWVPNKPDAATPVIPGPVGEMSVQYNTYLKQYLALYTNGMNDVVARTAPAPQGPWSAEQMLVSSWQMPGGIYAPMMHPWSTGKDVYFNLSLWSAYNVMLMHTVLP
Prediction	61344034144514443143443434125324634515433451565444345342441644323433425333442343433344544442422455434243425443424444353435445542344541463454344663443334325264443423453454233522343324143434434443544242442443322323333442343444245434443334403330001000000000103335110000000000134241310000000102233012003033223232111111345420320042552541100000000000322000000002202450311000000020223142043234221132432222121132342000001143531000000012214110000002264043252001111442102444452010014230000000115301000000013310000000440512144332103245211000000000003041000000000100000010518
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHCCCCCCCCCEEEEEEHCCCCCCCCCCCCCCCCEEEEHCCCCCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHCCCEEEHCCEEEEEEEEHCCCCCCCCEEEEEEEEEEHCCCCCCCEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEHCCCCCEEEEEHCCCCCCCCEEEEEHCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCCEEEEEEHCCCCCEEEEHCCCCCCCCCCCEEEHCCCCCCCCCCCCCHCCCCCCCEEEEEEEHCCCCCEEEEEEHCC
MYRYQVRVQQRRSEMNRWVATRSRRHTYQWITDHKSPRDHYRHISELRTSIATSSPGRCDMSPIPRIVSVSLAWAAAIGLMVPIGLAPPAMAAPCSGDAANAPPPPSAIVTDPGATALGPVRPGHGPIPTGRKPRGANDRAPLPKLGPLISALLNPGARNAAPLQQQALVPRANPGPNPAPNPPATGPQPPNATQLTPNPAPAPDPAPAAAPDPGATLAGATTSLAEWVTGPDSPNKTLERFGISGTDLGIPWDNGDPANRQVLMIFGDTFGYCAVDGHQWRYNTLFRSQDRDLGNGVHVTSGDASNRYSGSPVRQPGFSKQLINSIKWARDETGIIPTAGIAVGKTQYVNFMSIRNWGRDGEWTTNYSGIAVSKDNGQTWGVFPGTIRASGPDSGGKARFVPGNENFQMGAYLKSNDGYLYSFGTPPGRGGSAYLARVPQRFVPDLTKYQYWNGDSNSWVPNKPDAATPVIPGPVGEMSVQYNTYLKQYLALYTNGMNDVVARTAPAPQGPWSAEQMLVSSWQMPGGIYAPMMHPWSTGKDVYFNLSLWSAYNVMLMHTVLP

3chnS

0.10

0.96

1.24

MUSTER

--KSPIFG---PEEVNSVEGNSVSTCYYPPTSVNRHTRKYWCRQGARGGCITLISSEGYVSSKYAGRANLTNFPENGTFVVNIAQLSQDDSGRYKCGLGINSRGLSFDVSLEVSQGPGLLNDTKVYTVDLGRTVTINCPFKTENAQKRKSLYKQIGLYPVLVIDSSGYVNPNYTGRIRLDIQGTGQLLFSVVINQLRLSDAGQQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCALGPEVANVAKFLCRQSSGENCDVVV-----NTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKEDGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTVVGVAGSSVAVLCPYNRKESKSIKYWC--------LWEGAQNGRCPLLVDSEGWVKAQYEGRLSLLEEPGNGTFLTSRDAGFYWCLTNGDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEK--YWCKWNNTGCQALPSQDEGPSKAFVN---CDENSRLVSLTLNLVTRADEGWYWCGVQGHFYGETAAVYVAVEERKAAGSRDVSLA

3cm9S

0.07

0.93

1.66

dPPAS

TLISSEGYVSSKYAGRANLTNFPENGTFVVNIAQLSQDDGLGINSRGLSFDVSLEVSQGPGLLNDTKVYTVDLGRTVTINCPFKTENAQKRKSLYKQIGLYPVLVIDSSGYVNPNYTGRIRLDIQGTGQLLFSVVINQLRLSDAGQYLCQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCALGPEVANVAKFLCRQSSGENCDVVVNTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKEDAGRYLCGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTVVKGVAGSSVAVLCPYNRKESKSIKYWCLWEGAQNGRCPLLVDSEGWVKAQYEGRL-----------SLLEEPGNGTFTVILNQL-TSRDAGFYWCLTNGDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEKYWCKWNNTGCQALPSQDEGPSKAFVNCDENSRLVSLTLNL------VTRADEGWYWCGVKQGHFYGETAAVYVAVEERKAAGSRDVSLAKADAAPDEKVLDSGFRPR----------------------

4hbsA

0.12

0.07

0.61

1.81

wdPPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NSEVKPQIDSEWNLELPNRSGQYWKVFVYKDLKYNALFTRSLGWNGGDGVFTTGL---PDGNIFWSFNDSFENRSRGNCSFPRNSIVQTPGEKDENLVWLATNDPNADRYYQVRTHIRHPKATLSDEGEIDQDYLYWAGDATIYNNQQLWGAVDNTD---PNNLRRFGTCLATYSLEGKPGDATYKLISRND---------NFNDHTLGYGDTWEDEDGHIYLYTTS---NYKVAVARTATRDLG--SQWEYYVADPQGWTTQYPSTQDAENSTIIPESACSPWVFKGDTYYIGQSWFGRDVLFRSKHPYGPFVDQKTLFTLPEFLDKHVYVNIHP-SRTGELVISTNT-DFYRPYFYRVFLP

2ebsB

0.10

0.09

0.96

1.12

wMUSTER

FYVSTIYESPFPMGANDMGRNNGERNPFNSNSDRAKTWTNVTSIPDAFTNGIG-DPERNGTATAPQGEPVAGQPSSWLNRTTGAFPDKKPASIAPQPMKVALTPNFADYPGPWGVTFRQNRTSGAWDDITPRVGNSSPAPYNNQTFPAGGFCGLSVDATNPNRLVVITLDRDPGPALDSTDAGATWKDVTQLSSPSNLEGNWGHPTNAARYKDGTPVPWLDFNNGPQW--GGYGAPHGTPGLTKFGWWMSAVLIDPFNPEHLMYGTGATIWATDTLSRVEKDWAPSWYLQIDGIEENAILSLRSPKSGAAISGMKHDDLTKPQKMFGAPQFSNLDSI---DAAGNFPNVVVRAGSSG----HEYDSACARGAYATDGGDAWTIFPTCPPGMNASHYQGSTIDASGSQIVWSTKLDEQASGPWYSGKLKAQTANVLSDKVQDGTFDGGKFFVSTDG-GKSYAAKGAG----LVTGTSLMPAVNPWVAGDVWVPVP----EGGLFHSTDF--GAFGTASVGAPKSSAPSAVF--IWGTDKPGSDIGLYRSDWNYSGPTMIEADPK

4hbsA

0.14

0.08

0.56

1.52

wPPAS

-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NSEVKPQIDSEELPNRSGQYWKV-GGDLP---------DGNIFWSFNDSFENRSRGNCSFPRNIVQTPGEKDLADYVQTNDPNADRYYQTHIRHPKATLSDEKIQAGEIDQDYLYWAGDATIYNNQQLWGAVDNTD---PNNLRRFGTCLATYSLEGKPGDATYKLISRND---------NFNDHTLGYGDTWEDEDGHIYLYTTSNYK---VAVARTATRDLG--SQWEYYVADPQGWTTQYPSTQDAENSTIIPESACSPWVFKGDTYYIGQSWFGRDVLFRSKHPYGPFVDQKTLFTIGEQRYQHVYVNIHP-SRTGELVISTNT-DFYRVFLYDNDAP

4nl6A

0.09

0.04

0.43

2.91

dPPAS2

SFKHALKNGDICETSGKPKTTPKRKPAKKNKSQKKNTAASLQQWKVGDKCSAIWSPATIASIDFKRETCVVVYTGYGNREEQNLSDLLSPICEVANNIEQNAQENENESQVSTDESENSRSPGNKSDNIKPKSAPWNSFLPPPPPMPGPRLGPGKPGLKFNGPPPPPPPPPPHLLSCWLPPFPSGPPIIPPPPPICPDSLDDADALGSMLISWYMSGYHTGYYMGFRQNQKEGRCSHSLN-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

3chnS

0.11

0.10

0.96

1.53

PPAS

--KSPIFG---PEEVNSVEGNSVSTCYYPPTSVNRHTRKYWCRQGARGGCITLISSEGYVSSKYAGRANLTNFPENGTFVVNIAQLSQDDSGRYKCGLGINSRGLSFDVSLEVSQGPGLLNDTKVYTVDLGRTVTINCPFKTENAQKRKSLYKQIGLYPVLVIDSSGYVNPNYTGRIRLDIQGTGQLLFSVVINQLRLSDAGQQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCALGPEVANVAKFLCRQSSGENCDVVV--NTLGK---RAPAFEGRILLNPQDKDGSFSVVITGLRKEDGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTV-GVAGSSVAVLCPYNRKESKSIKYWC--------LWEGAQNGRCPLLVDSEGWVKAQYEGRLSLLEEPGNGTFLTSRDAGFYWCLTNGDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEK---WCKWNNTGCQALPSQDEGPSK---AFVNCDENSRLVSLTLNLVTRADEGWYWCGVQGHFYGETAAVYVAVEERKAAGSRDVSLA

4hbsA

0.12

0.07

0.60

1.64

Env-PPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NSEVKPQIDSEWNLELPNRSGQYWKVFVYKDLKYNALFTRSLGWNGGDGVFTTGL---PDGNIFWSFNDSFENRSRGNCSFPRNIVQTPGEKDLADYVQTNDPNADRYYTHIRHPKATLSDEKIQAGEIDQD-YLYWAGDATIYNNQQLWGAVDNTDPNNLR-RFGTCLATYSLEGKPGDATYKLISRND-----------NFNDHTLGYGDTWEDEDGHIYLYTTSNYK---VAVARTATRDL--GSQWEYYVADPQGWTTQYPSTQDAENSTIIPESACSPWVFKGDTYYIGQSWFGRDVLFRSKHPYGPFVDQKTLFTLPELDKIGEQRNIHPASRTGELVISTNT-DCSNFWDNF-NAP

4yw0A

0.09

0.97

1.12

MUSTER

GGGENVTKELKDKFTSKYNQSSEKGLISNSKPGQQNSYRDNGELTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTGTKVVEKKVDNFLNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHLIYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTWTKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEK----KNKQVLMFADVMPAGVSFREATRKDSGYKQIDGNYYL--KLRKQGDTDYNYT---IRENGT---VYDDRTNRPTEFSVDKNFGIKQNGYLTVEQYSVSFFYKDALFKTNYIAYISSNDHGESWTLLPPIMGLNRNAPYLGPRGIIESSTGRILIPSYTGKESAFIYSDDNGWSAEAQFVELSPGVINNGKIAYLTSKDAGTWSAPEYLKFVSNPSYGTQLSIINYSQLIDAVILSTPNSTNGIWIGLINDDNTIWRYHHDVDYSNYGYS--Y--TLTELPNHE-IGLMFEKFDSWSVPYITFKIE

3chnS

0.07

0.06

0.93

1.65

dPPAS

TLISSEGYVSSKYAGRANLTNFPENGTFVVNIAQLSQDDGLGINSRGLSFDVSLEVSQGPGLLNDTKVYTVDLGRTVTINCPFKTENAQKRKSLYKQIGLYPVLVIDSSGYVNPNYTGRIRLDIQGTGQLLFSVVINQLRLSDAGQYLCQAGDDSNSNKKNADLQVLKPEPELVYEDLRGSVTFHCALGPEVANVAKFLCRQSSGENCDVVVNTLGKRAPAFEGRILLNPQDKDGSFSVVITGLRKEDAGRYLCGAHSDGQLQEGSPIQAWQLFVNEESTIPRSPTVVKGVAGSSVAVLCPYNRKESKSIKYWCLWEGAQNGRCPLLVDSEGWVKAQYEGRL-----------SLLEEPGNGTFTVILNQL-TSRDAGFYWCLTNGDTLWRTTVEIKIIEGEPNLKVPGNVTAVLGETLKVPCHFPCKFSSYEKYWCKWNNTGCQALPSQDEGPSKAFVNCDENSRLVSLTLNLVT-------RADEGWYWCGVKQGHFYGETAAVAAGSRDVSLAKADAAPDEKVLDSGFREIENKAIQDPR--------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-0.75

Estimated TM-score = 0.62±0.14

Estimated RMSD = 9.3±4.6Å

Download Model 2

C-score=-2.00

Download Model 3

C-score=-3.32

Download Model 4

C-score=-3.71

Download Model 5

C-score=-3.72

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3chnS	0.775	4.33	0.094	0.929
2	3poyA	0.391	7.85	0.034	0.646
3	5a22A	0.380	7.97	0.049	0.636
4	1ej6A	0.376	7.54	0.042	0.599
5	3cu7A	0.372	7.73	0.051	0.615
6	5dotA	0.371	8.19	0.037	0.641
7	5l56A	0.370	7.91	0.028	0.613
8	3k1qA	0.370	7.97	0.051	0.623
9	1hn0A	0.363	7.98	0.059	0.613
10	3jb9A	0.363	8.14	0.049	0.618

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.04	2	4yhoL	4CC	Rep, Mult	40,97,99
2	0.04	2	1pjxA	MXE	Rep, Mult	463,464
3	0.02	1	5drkA	BCT	Rep, Mult	67,68
4	0.02	1	4ac1X	NAG	Rep, Mult	450,453,454
5	0.02	1	3cmvE	ANP	Rep, Mult	160,161,162,220
6	0.02	1	2bf6A	CA	Rep, Mult	457,458,513
7	0.02	1	4j6rL	CA	Rep, Mult	130,198
8	0.02	1	2atpB	III	Rep, Mult	6,8,29,105
9	0.02	1	1nhcC	SO4	Rep, Mult	271,272,273,308
10	0.02	1	3cmvB	ANP	Rep, Mult	68,83,85,112
11	0.02	1	1z1nX	ZN	Rep, Mult	353,358
12	0.02	1	3lxvN	CO3	Rep, Mult	360,362
13	0.02	1	3g31B	GF1	Rep, Mult	41,42
14	0.02	1	2zkrq	NUC	Rep, Mult	142,143
15	0.02	1	3ubeC	GAL	Rep, Mult	33,36
16	0.02	1	1h9wA	MN	Rep, Mult	460,484,535
17	0.02	1	4cuyU	III	Rep, Mult	150,156
18	0.02	1	1htwA	MG	Rep, Mult	268,269
19	0.02	1	1hn1B	NA	Rep, Mult	200,203,218
20	0.02	1	1nipA	MG	Rep, Mult	243,245

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1f13A	0.299	7.23	0.045	0.465	2.3.2.13	NA
2	0.060	2pffA	0.307	7.81	0.052	0.508	2.3.1.41,2.3.1.86	NA
3	0.060	1zvuA	0.316	7.43	0.032	0.506	5.99.1.-	NA
4	0.060	2e28A	0.299	7.43	0.041	0.476	2.7.1.40	194
5	0.060	3ecqB	0.328	7.95	0.039	0.549	3.2.1.97	195,199
6	0.060	3btaA	0.356	8.27	0.046	0.618	3.4.24.69	NA
7	0.060	2np0A	0.363	8.30	0.036	0.639	3.4.24.69	NA
8	0.060	1z0hB	0.214	7.12	0.037	0.330	3.4.24.69	NA
9	0.060	2fhbA	0.356	7.07	0.048	0.547	3.2.1.41	NA
10	0.060	1llwA	0.313	7.77	0.048	0.511	1.4.7.1	NA
11	0.060	2qf7A	0.308	7.73	0.024	0.499	6.4.1.1	NA
12	0.060	3iydC	0.308	8.11	0.060	0.520	2.7.7.6	92
13	0.060	2hpiA	0.313	8.04	0.035	0.533	2.7.7.7	141
14	0.060	2fhcA	0.353	7.17	0.066	0.545	3.2.1.41	70
15	0.060	2pffB	0.353	8.07	0.071	0.593	2.3.1.86	243,278
16	0.060	3lxuX	0.325	6.99	0.025	0.487	3.4.14.10	222,239
17	0.060	1f1sA	0.296	8.19	0.062	0.510	4.2.2.1	NA
18	0.060	1yq2A	0.318	7.79	0.030	0.526	3.2.1.23	NA
19	0.060	1w36C	0.290	7.96	0.047	0.494	3.1.11.5	244

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.15	0.775	4.33	0.09	0.93	3chnS	GO:0001580 GO:0001792 GO:0001895 GO:0002415 GO:0005576 GO:0005615 GO:0005886 GO:0005887 GO:0007173 GO:0016020 GO:0016021 GO:0038093 GO:0043113 GO:0043235 GO:0070062
1	0.06	0.299	7.64	0.04	0.48	3dmkA	GO:0003823 GO:0005887 GO:0006909 GO:0007411 GO:0007413 GO:0007419 GO:0007422 GO:0008046 GO:0016020 GO:0016021 GO:0016319 GO:0021551 GO:0030424 GO:0030425 GO:0032490 GO:0042802 GO:0042803 GO:0043005 GO:0043025 GO:0048666 GO:0048814 GO:0048846 GO:0050770 GO:0050976 GO:0070593
2	0.06	0.262	7.75	0.04	0.42	3b43A
3	0.06	0.233	7.27	0.06	0.36	2v5yA	GO:0001937 GO:0004721 GO:0004725 GO:0005001 GO:0005737 GO:0005887 GO:0005911 GO:0005913 GO:0006470 GO:0007155 GO:0007156 GO:0007165 GO:0010596 GO:0010842 GO:0016020 GO:0016021 GO:0016311 GO:0016525 GO:0016787 GO:0016791 GO:0030027 GO:0031175 GO:0031290 GO:0035335 GO:0042493 GO:0042802 GO:0045296 GO:0045909 GO:0048471
4	0.06	0.257	4.52	0.07	0.31	3ghbH
5	0.06	0.251	4.52	0.04	0.30	2ig2H	GO:0003823 GO:0004252 GO:0005576 GO:0005886 GO:0006508 GO:0006898 GO:0006955 GO:0006956 GO:0006958 GO:0038095 GO:0038096 GO:0050776 GO:0070062
6	0.06	0.251	4.66	0.05	0.31	1ynkH
7	0.06	0.248	4.36	0.05	0.30	1indH
8	0.06	0.248	4.43	0.04	0.30	2x7lB
9	0.06	0.243	4.75	0.03	0.30	3mnvA
10	0.06	0.246	4.74	0.04	0.31	2oz4L
11	0.06	0.239	4.21	0.05	0.28	3p30H
12	0.06	0.248	4.60	0.03	0.30	1gpoM	GO:0003823 GO:0005886 GO:0006910 GO:0006911 GO:0006958 GO:0009897 GO:0030183 GO:0034987 GO:0042571 GO:0042742 GO:0045087 GO:0050853 GO:0050871 GO:0072562
13	0.06	0.245	4.66	0.05	0.30	3clfH
14	0.06	0.249	4.56	0.04	0.30	3m8oL
15	0.06	0.256	6.64	0.04	0.38	2jllA	GO:0005886 GO:0007155 GO:0007158 GO:0016020 GO:0016021 GO:0030424
16	0.06	0.246	4.61	0.05	0.30	3mlrH
17	0.06	0.242	4.54	0.05	0.29	1e4xI
18	0.06	0.237	4.50	0.05	0.29	3fmgH

Consensus prediction of GO terms

Molecular Function	GO:0001792	GO:0008046	GO:0003823	GO:0042803	GO:0045296	GO:0005001
GO-Score	0.15	0.06	0.06	0.06	0.06	0.06
Biological Processes	GO:0001580	GO:0043113	GO:0007173	GO:0001895	GO:0038093	GO:0002415	GO:0032490	GO:0048814	GO:0007419	GO:0050770	GO:0007413	GO:0021551	GO:0048846	GO:0016319	GO:0006909	GO:0007422	GO:0050976	GO:0070593	GO:0035335	GO:0042493	GO:0045909	GO:0001937	GO:0007156	GO:0010596	GO:0010842	GO:0031290	GO:0016525
GO-Score	0.15	0.15	0.15	0.15	0.15	0.15	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06	0.06
Cellular Component	GO:0031226
GO-Score	0.50

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.