I-TASSER results

I-TASSER results for job id Rv1648

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (268 residues)
MIYRVACLLARIRFTVGYVAALASVSTTILMHGPQVHAQVIRHASTNLHNLAHGHLGTLW
NSAFVIDEGPLYFWLPCLACLLAVAELQLRSLRLTVAFVVGHIGATLLVAAVLAGAIEIG
WLPWSISRVSDVGMSYGALAALGALTAAIPGRWRPAWIGWWVSLGLATATIGGGFTDAGH
TVALLLGMLVTACFTRPARWTLGRCALLAVASGFCLVLLAHSWWSLVSGSALGLLGALGA
AGFARWTRARATSLPPGALAIPQPALSR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MIYRVACLLARIRFTVGYVAALASVSTTILMHGPQVHAQVIRHASTNLHNLAHGHLGTLWNSAFVIDEGPLYFWLPCLACLLAVAELQLRSLRLTVAFVVGHIGATLLVAAVLAGAIEIGWLPWSISRVSDVGMSYGALAALGALTAAIPGRWRPAWIGWWVSLGLATATIGGGFTDAGHTVALLLGMLVTACFTRPARWTLGRCALLAVASGFCLVLLAHSWWSLVSGSALGLLGALGAAGFARWTRARATSLPPGALAIPQPALSR
Prediction	CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
Conf.Score	9875788888765899999999999999998786788888887668877765487455543343336874789999999999999998767999999999999999999999999998777656665534555479999999999997776579999999999999998867974689999999999999998668888875578889999999999997556643456776655667776655544445667887766667776679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MIYRVACLLARIRFTVGYVAALASVSTTILMHGPQVHAQVIRHASTNLHNLAHGHLGTLWNSAFVIDEGPLYFWLPCLACLLAVAELQLRSLRLTVAFVVGHIGATLLVAAVLAGAIEIGWLPWSISRVSDVGMSYGALAALGALTAAIPGRWRPAWIGWWVSLGLATATIGGGFTDAGHTVALLLGMLVTACFTRPARWTLGRCALLAVASGFCLVLLAHSWWSLVSGSALGLLGALGAAGFARWTRARATSLPPGALAIPQPALSR
Prediction	7333014102202001000211110010012134511330043212304204723010000000112533121111220000010023122310000001001100120001000002223114411302000000000000001001123312210111011110000012230120002200110110011034443243232112000001010011133233233333222111102102313444445255442335443368
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
MIYRVACLLARIRFTVGYVAALASVSTTILMHGPQVHAQVIRHASTNLHNLAHGHLGTLWNSAFVIDEGPLYFWLPCLACLLAVAELQLRSLRLTVAFVVGHIGATLLVAAVLAGAIEIGWLPWSISRVSDVGMSYGALAALGALTAAIPGRWRPAWIGWWVSLGLATATIGGGFTDAGHTVALLLGMLVTACFTRPARWTLGRCALLAVASGFCLVLLAHSWWSLVSGSALGLLGALGAAGFARWTRARATSLPPGALAIPQPALSR

2ic8A

0.14

0.09

0.64

1.22

MUSTER

---------ERAGVTWVMMIACVVVFIAMQILG---DQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSG--------------PWFGGLSGVVYALMGYVWLRGERDPQSRGLIIFALIWIVAGWFGMSMANGAHIAGLAVGLAMAFVDSLNA----------------------------------------------------------------------

2ic8A

0.13

0.08

0.65

0.97

dPPAS

--------ERAGPVTWVMMIACVVVFIAMQILG---DQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSG--------------PWFGGLSGVVYALMGYVWLRGERDPQSRGLIIFALIWIVAGWFDMSMANGAHIAGLAVGLAMAFVDSLNA----------------------------------------------------------------------

2ic8A

0.13

0.09

0.64

2.28

wdPPAS

---------ERAGVTWVMMIACVVVFIAMQILG---DQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSG--------------PWFGGLSGVVYALMGYVWLRGERDPQSRGLIIFALIWIVAGWFDMSMANGAHIAGLAVGLAMAFVDSLNA----------------------------------------------------------------------

2ic8A

0.14

0.09

0.64

1.65

wMUSTER

---------ERAGVTWVMMIACVVVFIAMQILG---DQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSG--------------PWFGGLSGVVYALMGYVWLRGERDPQSRGLIIFALIWIVAGWFGMSMANGAHIAGLAVGLAMAFVDSLNA----------------------------------------------------------------------

2ic8A

0.14

0.09

0.63

2.96

wPPAS

---------ERAGVTWVMMIACVVVFIAMQILG---DQEVMLWLAWPFDPTLKFEFWRYFTHALMHFS-HILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFS---------------PWFGGLSGVVYALMGYVWLRGERDPQSRGLIIFALIWIVAGWFDMSMANGAHIAGLAVGLAMAFVDSLNA----------------------------------------------------------------------

2ic8A

0.13

0.09

0.64

2.12

dPPAS2

---------ERAGVTWVMMIACVVVFIAMQILG---DQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWF--------------GGLSGVVYALMGYVWLRGERDPQSRGLIIFALIWIVAGWFDMSMANGAHIAGLAVGLAMAFVDSLNA----------------------------------------------------------------------

2ic8A

0.13

0.09

0.64

1.87

PPAS

---------ERAGVTWVMMIACVVVFIAMQILG---DQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSG--------------PWFGGLSGVVYALMGYVWLRGERDPQSRGLIIFALIWIVAGWFDMSMANGAHIAGLAVGLAMAFVDSLNA----------------------------------------------------------------------

2ic8A

0.13

0.09

0.64

2.19

Env-PPAS

---------ERAGVTWVMMIACVVVFIAMQILG---DQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSG--------------PWFGGLSGVVYALMGYVWLRGERDPQSRGLIIFALIWIVAGWFDMSMANGAHIAGLAVGLAMAFVDSLNA----------------------------------------------------------------------

2nr9A

0.13

0.09

0.68

1.16

MUSTER

FLAQQ------GKITLILTALCVLIYIAQQLGF---EDDIMYLMHYPAYEEQDSEVWRYISHTLVHLSLHILFNLSWFFIFGGMIERTFGSVKLLMLYVVASAITGYVQNYVSG--------------PAFFGLSGVVYAVLGYVFIRDKLNHHLTMLLVGIALGFISPLFGVEMGNAAHISGLIVGLIWGFIDSKLRK---NSLELVP-----------------------------------------------------------

2nr9A

0.16

0.11

0.68

0.82

dPPAS

-------FLAQQGITLILTALCVLIYIAQQLGFEDDIMYLMHYPAY---EEQDSEVWRYISHTLVHLSLHILFNLSWFFIFGGMIERTFGSVKLLMLYVVASAITGYVQNYVSGPAF--------------FGLSGVVYAVLGYVFIRDKLNHHLTMLLVGIALGFISPLFGVEMGNAAHISGLIVGLIWGFIDSK-----LRKNSLELVP---------------------------------------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.31

Estimated TM-score = 0.44±0.14

Estimated RMSD = 11.3±4.5Å

Download Model 2

C-score=-3.09

Download Model 3

C-score=-3.40

Download Model 4

C-score=-3.36

Download Model 5

C-score=-4.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2ic8A	0.616	1.42	0.121	0.645
2	2nr9A	0.615	2.04	0.120	0.668
3	4av3A	0.506	5.55	0.053	0.821
4	1hxgA	0.504	5.76	0.055	0.802
5	1n1zA	0.502	5.65	0.063	0.798
6	3g4dA	0.484	5.92	0.068	0.802
7	2ongA2	0.484	5.54	0.052	0.761
8	5c05A	0.483	5.66	0.058	0.765
9	1e6yA	0.479	5.42	0.083	0.750
10	3saeA	0.474	5.78	0.048	0.765

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.13	7	5f5jA	III	Rep, Mult	41,67,70,74,109,113,130,131,132,133,135,173,174,175,176,180
2	0.10	5	4h1dA	DFP	Rep, Mult	70,74,132,133,134,135,136,139,165,180
3	0.06	3	3zmhA	L62	Rep, Mult	69,70,73,74,77,135,138,139,142,165,173,180
4	0.04	2	2xtvA	MC3	Rep, Mult	19,72,73,75,76,79
5	0.04	2	3m2rD	F43	Rep, Mult	84,85,89,90,142,145
6	0.04	2	1frfL	SF4	Rep, Mult	11,180
7	0.02	1	2xtvA	MC3	Rep, Mult	73,76,77,79,80,161
8	0.02	1	2xtvA	MC3	Rep, Mult	104,108,182,186,189
9	0.02	1	2xtvA	MC3	Rep, Mult	60,91,95,99
10	0.02	1	2xtvA	MC3	Rep, Mult	46,56,59,103,110
11	0.02	1	2nr9A	PA6	Rep, Mult	41,61

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.337	3b45A	0.609	1.41	0.123	0.638	3.4.21.105	21,63,73
2	0.060	2ouyA	0.459	5.89	0.047	0.757	3.1.4.17	76,169
3	0.060	1e1eB	0.448	5.97	0.053	0.743	3.2.1.21	NA
4	0.060	1qoxA	0.449	5.88	0.063	0.731	3.2.1.21	NA
5	0.060	1tr1D	0.404	6.09	0.064	0.698	3.2.1.21	NA
6	0.060	3b8eA	0.407	6.03	0.034	0.698	3.6.3.9	NA
7	0.060	1myrA	0.459	5.88	0.040	0.746	3.2.1.147	NA
8	0.060	1e6vA	0.468	5.30	0.084	0.713	2.8.4.1	NA
9	0.060	1fpsA	0.464	5.29	0.067	0.713	2.5.1.1,2.5.1.10	NA
10	0.060	1mo7A	0.251	5.87	0.058	0.403	3.6.3.9	NA
11	0.060	3g4dA	0.484	5.92	0.068	0.802	4.2.3.13	NA
12	0.060	2nr9A	0.615	2.04	0.120	0.668	3.4.21.105	73,142
13	0.060	3b8eC	0.408	6.29	0.046	0.720	3.6.3.9	NA
14	0.060	1bgaA	0.452	5.89	0.059	0.731	3.2.1.21	NA
15	0.060	1rqiA	0.451	5.80	0.091	0.716	2.5.1.10	NA
16	0.060	2zoxA	0.432	5.30	0.065	0.657	3.2.1.21	84,89
17	0.060	1v03A	0.432	5.55	0.040	0.672	3.2.1.21	NA
18	0.060	1hbmB	0.451	5.16	0.073	0.679	2.8.4.1	NA
19	0.060	3ixzA	0.465	5.55	0.058	0.728	3.6.3.10	NA
20	0.060	2e9lA	0.452	5.94	0.054	0.739	3.2.1.21	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.22	0.539	1.59	0.13	0.57	3odjA	GO:0004252 GO:0005886 GO:0006508 GO:0008233 GO:0008236 GO:0016020 GO:0016021 GO:0016485 GO:0016787 GO:0042802
1	0.20	0.608	1.59	0.12	0.65	5f5bA	GO:0004175 GO:0004252 GO:0005886 GO:0005887 GO:0006508 GO:0008233 GO:0008236 GO:0016020 GO:0016021 GO:0016485 GO:0016787
2	0.14	0.615	2.04	0.12	0.67	2nr9A	GO:0004252 GO:0005886 GO:0006508 GO:0008233 GO:0008236 GO:0016020 GO:0016021 GO:0016485 GO:0016787 GO:0042802
3	0.07	0.378	6.01	0.06	0.62	4av3B	GO:0000287 GO:0004427 GO:0005509 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006814 GO:0009678 GO:0015081 GO:0015992 GO:0016020 GO:0016021 GO:0016787 GO:0030955 GO:0035725 GO:0042803 GO:0046872
4	0.07	0.406	5.71	0.04	0.65	2ongA	GO:0000287 GO:0008152 GO:0010333 GO:0016829 GO:0046872
5	0.07	0.488	5.54	0.06	0.76	5c05B	GO:0000287 GO:0008152 GO:0010333 GO:0016829 GO:0046872
6	0.07	0.474	5.78	0.05	0.76	3saeA	GO:0000287 GO:0005737 GO:0008152 GO:0010333 GO:0016829 GO:0046872 GO:0052681
7	0.07	0.502	5.65	0.06	0.80	1n1zA	GO:0000287 GO:0008152 GO:0009507 GO:0009536 GO:0010333 GO:0016829 GO:0016853 GO:0046211 GO:0046872 GO:0047926
8	0.07	0.444	5.90	0.05	0.73	3n0fA	GO:0000287 GO:0008152 GO:0009507 GO:0009536 GO:0010333 GO:0016829 GO:0034009 GO:0046872
9	0.06	0.389	5.98	0.05	0.65	3s9vB	GO:0000287 GO:0003824 GO:0008152 GO:0009507 GO:0009536 GO:0010333 GO:0016829 GO:0016853 GO:0046872 GO:0050554 GO:0050559
10	0.06	0.384	5.73	0.02	0.63	2j5cB	GO:0000287 GO:0008152 GO:0010333 GO:0016829 GO:0046872
11	0.06	0.463	5.48	0.09	0.74	4a01A	GO:0004427 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0009678 GO:0015992 GO:0016020 GO:0016021 GO:0016787 GO:0046872 GO:0055085
12	0.06	0.472	5.90	0.07	0.77	4gaxA	GO:0000287 GO:0008152 GO:0010333 GO:0016829 GO:0034006 GO:0046872
13	0.06	0.513	5.49	0.06	0.82	4av6A	GO:0000287 GO:0004427 GO:0005509 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006814 GO:0009678 GO:0015081 GO:0015992 GO:0016020 GO:0016021 GO:0016787 GO:0030955 GO:0035725 GO:0042803 GO:0046872
14	0.06	0.509	5.72	0.06	0.81	5dhiA	GO:0000287 GO:0005737 GO:0008152 GO:0010333 GO:0016114 GO:0016829 GO:0046872
15	0.06	0.484	5.92	0.07	0.80	3g4dA	GO:0000287 GO:0008152 GO:0010333 GO:0016114 GO:0016829 GO:0046872 GO:0047461
16	0.06	0.355	6.70	0.05	0.64	5bv7A	GO:0004607 GO:0004623 GO:0005576 GO:0005615 GO:0006629 GO:0006644 GO:0006656 GO:0008202 GO:0008203 GO:0008374 GO:0016740 GO:0016746 GO:0030301 GO:0034186 GO:0034364 GO:0034372 GO:0034375 GO:0034435 GO:0042157 GO:0042158 GO:0042632 GO:0043691 GO:0046470 GO:0046688 GO:0051384 GO:0070062 GO:0090107
17	0.06	0.367	5.73	0.06	0.59	3fhnA	GO:0005783 GO:0005789 GO:0006810 GO:0006890 GO:0015031 GO:0016020 GO:0016192 GO:0048193 GO:0070939
18	0.06	0.314	5.99	0.08	0.53	4ylfA	GO:0005737 GO:0006221 GO:0016491 GO:0046872 GO:0050660 GO:0051536 GO:0051537 GO:0055114

Consensus prediction of GO terms

Molecular Function	GO:0004252	GO:0042802
GO-Score	0.46	0.33
Biological Processes	GO:0016485
GO-Score	0.46
Cellular Component	GO:0031226	GO:0016021
GO-Score	0.50	0.49

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.