I-TASSER results

I-TASSER results for job id Rv1619

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (484 residues)
MVAAAGEPLNCQRANPEVTVKLPSADVVPRLRGRQRVVVHVDSRTARCVGALALVCAACW
LIALLAGDYRHAQWAVAGRLGWSLTVLAAVAFIARGIFLGRPVTAMHATAAGLFLLAGLA
AHVLVADLLGEILIAGSGWALMWPTSAHPRPEDLPRVWALINATRADSLAPFAMQAGKSH
HFSAAGTAALAYRTRIGYAVVSGDPIGDEAQFPQLVADFAAMCHMHGWRIVVVGCSERRL
GLWSDPMVVGQSLRPIPIGRDVVIDVSNFEMTGRRFRNLRQAVKRTHNFGVTTEIVAEQQ
LDDQRQAELAEVLAASPSGARTDRGFCMNLDGVLEGRYPGIQLIIARDASGRVQGFHRYA
TAGGGSDMSLDVPWRRRGAPNGIDERLSADMIAAAKDAGVQRLSLAFAAFPDLFGANQLG
RLQRVCRALIHILDPLIALESLYRYLRKFHALDERRYVLISMTQVFALALVLLSLEFVPR
RRHL

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MVAAAGEPLNCQRANPEVTVKLPSADVVPRLRGRQRVVVHVDSRTARCVGALALVCAACWLIALLAGDYRHAQWAVAGRLGWSLTVLAAVAFIARGIFLGRPVTAMHATAAGLFLLAGLAAHVLVADLLGEILIAGSGWALMWPTSAHPRPEDLPRVWALINATRADSLAPFAMQAGKSHHFSAAGTAALAYRTRIGYAVVSGDPIGDEAQFPQLVADFAAMCHMHGWRIVVVGCSERRLGLWSDPMVVGQSLRPIPIGRDVVIDVSNFEMTGRRFRNLRQAVKRTHNFGVTTEIVAEQQLDDQRQAELAEVLAASPSGARTDRGFCMNLDGVLEGRYPGIQLIIARDASGRVQGFHRYATAGGGSDMSLDVPWRRRGAPNGIDERLSADMIAAAKDAGVQRLSLAFAAFPDLFGANQLGRLQRVCRALIHILDPLIALESLYRYLRKFHALDERRYVLISMTQVFALALVLLSLEFVPRRRHL
Prediction	CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHCCCEEEHCCCCCEEEEEEEHCCEEEEHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEHCHHHHHHHHHHHHHHHCCEEEEHCCEEEEHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHCCCCCCCCCCCEEEEEHCCCCEEEEEEEEEHCCCCCEEEEEEEHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEHCCCCCHHHHHHHHHHHHCCCCCCC
Conf.Score	9887766545777778899999999999999974468887675789999999999999999999988754555667777899999999999999887655776666665566666545478999999999999999999998477888996899999999999889875445664996899958985999999988999998799999889999999999999999998999986879999998776666587588765557875677888885589999999988774979999977889999999999999999986898765788756788855567669999958996999999998589985899987658999985999999999999999899899666555777888888879999999999999887675779999998698764689998599858999999999976777889
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MVAAAGEPLNCQRANPEVTVKLPSADVVPRLRGRQRVVVHVDSRTARCVGALALVCAACWLIALLAGDYRHAQWAVAGRLGWSLTVLAAVAFIARGIFLGRPVTAMHATAAGLFLLAGLAAHVLVADLLGEILIAGSGWALMWPTSAHPRPEDLPRVWALINATRADSLAPFAMQAGKSHHFSAAGTAALAYRTRIGYAVVSGDPIGDEAQFPQLVADFAAMCHMHGWRIVVVGCSERRLGLWSDPMVVGQSLRPIPIGRDVVIDVSNFEMTGRRFRNLRQAVKRTHNFGVTTEIVAEQQLDDQRQAELAEVLAASPSGARTDRGFCMNLDGVLEGRYPGIQLIIARDASGRVQGFHRYATAGGGSDMSLDVPWRRRGAPNGIDERLSADMIAAAKDAGVQRLSLAFAAFPDLFGANQLGRLQRVCRALIHILDPLIALESLYRYLRKFHALDERRYVLISMTQVFALALVLLSLEFVPRRRHL
Prediction	7121013301242013001000000000000100210103132300100001103222222221101101243133221000000110021000000000121201320101000121100001211222222222120000122433346522630350066126210000001311100004622000000231200000000003573034004201510372400000000135003103233135220200100120203076051615514500410330543402010233650356025203500430266455220000101111242012000000004732000000000023420000000022560130000100010031027340420000000023035663120021003100410230030400230262041522000000144210000000002000043467
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHCCCEEEHCCCCCEEEEEEEHCCEEEEHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEHCHHHHHHHHHHHHHHHCCEEEEHCCEEEEHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHCCCCCCCCCCCEEEEEHCCCCEEEEEEEEEHCCCCCEEEEEEEHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEEHCCCCCHHHHHHHHHHHHCCCCCCC
MVAAAGEPLNCQRANPEVTVKLPSADVVPRLRGRQRVVVHVDSRTARCVGALALVCAACWLIALLAGDYRHAQWAVAGRLGWSLTVLAAVAFIARGIFLGRPVTAMHATAAGLFLLAGLAAHVLVADLLGEILIAGSGWALMWPTSAHPRPEDLPRVWALINATRADSLAPFAMQAGKSHHFSAAGTAALAYRTRIGYAVVSGDPIGDEAQFPQLVADFAAMCHMHGWRIVVVGCSERRLGLWSDPMVVGQSLRPIPIGRDVVIDVSNFEMTGRRFRNLRQAVKRTHNFGVTTEIVAEQQLDDQRQAELAEVLAASPSGARTDRGFCMNLDGVLEGRYPGIQLIIARDASGRVQGFHRYATAGGGSDMSLDVPWRRRGAPNGIDERLSADMIAAAKDAGVQRLSLAFAAFPDLFGANQLGRLQRVCRALIHILDPLIALESLYRYLRKFHALDERRYVLISMTQVFALALVLLSLEFVPRRRHL

4v36A

0.17

0.11

0.67

2.26

MUSTER

-------------------------------------------------------------------------------------------------------------------------------------------------KEIGEEPDPEKLEAFLEEKGGNALSHLGFLGDKRFFYSSDGNALIQFAKVGQRLVVLGDPSGREDSFPLVIKEFLHAADQKGYLVIFYQIEREDMALYHDF-----GYRFFKLGEEAIVDLDTFTISGKKRAGLRAIYNRFEREGYTFHVEQP-PFSREFLNELRQVSDEWLGRK-KEKGFSL--GFFQEDYLQKAPIAVLKSEEGEIVAFMNIMPMYREGEISIDLMRYSKKAPKGIMDALFIYLFQWGKEQGYTAFNMGMAPLSNVGTSFWT---ERLAAVIFNNVSYMYSFSGLRSFKEKYKPVWRGKYLAYRKNRSLPVTMILVTRLIGRRTK--

4v36A

0.17

0.11

0.67

2.77

dPPAS

-------------------------------------------------------------------------------------------------------------------------------------------------KEIGEEPDPEKLEAFLEEKGGNALSHLGFLGDKRFFYSSDGNALIQFAKVGQRLVVLGDPSGREDSFPLVIKEFLHAADQKGYLVIFYQIEREDMALYHDF-----GYRFFKLGEEAIVDLDTFTISGKKRAGLRAIYNRFEREGYTFHVEQ-PPFSREFLNELRQVSDEWLGRK-KEKGFSL--GFFQEDYLQKAPIAVLKSEEGEIVAFMNIMPMYREGEISIDLMRYSKKAPKGIMDALFIYLFQWGKEQGYTAFNMGMAPLSNVGTSFWT---ERLAAVIFNNVSYMYSFSGLRSFKEKYKPVWRGKYLAYRKNRSLPVTMILVTRLIGRRTK--

4v36A

0.17

0.11

0.67

4.82

wdPPAS

-------------------------------------------------------------------------------------------------------------------------------------------------KEIGEEPDPEKLEAFLEEKGGNALSHLGFLGDKRFFYSSDGNALIQFAKVGQRLVVLGDPSGREDSFPLVIKEFLHAADQKGYLVIFYQIEREDMALYHDF-----GYRFFKLGEEAIVDLDTFTISGKKRAGLRAIYNRFEREGYTFHVEQ-PPFSREFLNELRQVSDEWLGRKK-EKGFSL--GFFQEDYLQKAPIAVLKSEEGEIVAFMNIMPMYREGEISIDLMRYSKKAPKGIMDALFIYLFQWGKEQGYTAFNMGMAPLSNVGT---SFWTERLAAVIFNNVSYMYSFSGLRSFKEKYKPVWRGKYLAYRKNRSLPVTMILVTRLIGRRTK--

4v36A

0.17

0.11

0.67

2.90

wMUSTER

----------------------------------------------------------------------------------------------------------------------------------------------KEI---GEEPDPEKLEAFLEEKGGNALSHLGFLGDKRFFYSSDGNALIQFAKVGQRLVVLGDPSGREDSFPLVIKEFLHAADQKGYLVIFYQIEREDMALYHDF-----GYRFFKLGEEAIVDLDTFTISGKKRAGLRAIYNRFEREGYTFHVEQ-PPFSREFLNELRQVSDEWLGRK-KEKGFSL--GFFQEDYLQKAPIAVLKSEEGEIVAFMNIMPMYREGEISIDLMRYSKKAPKGIMDALFIYLFQWGKEQGYTAFNMGMAPLSNVGTSFWT---ERLAAVIFNNVSYMYSFSGLRSFKEKYKPVWRGKYLAYRKNRSLPVTMILVTRLIGRRTK--

4v36A

0.17

0.11

0.67

5.05

wPPAS

-------------------------------------------------------------------------------------------------------------------------------------------------KEIGEEPDPEKLEAFLEEKGGNALSHLGFLGDKRFFYSSDGNALIQFAKVGQRLVVLGDPSGREDSFPLVIKEFLHAADQKGYLVIFYQIEREDMALYHDF-----GYRFFKLGEEAIVDLDTFTISGKKRAGLRAIYNRFEREGYTFHVEQ-PPFSREFLNELRQVSDEWLGRKK-EKGFSL--GFFQEDYLQKAPIAVLKSEEGEIVAFMNIMPMYREGEISIDLMRYSKKAPKGIMDALFIYLFQWGKEQGYTAFNMGMAPLSNVGT---SFWTERLAAVIFNNVSYMYSFSGLRSFKEKYKPVWRGKYLAYRKNRSLPVTMILVTRLIGRRTK--

4v36A

0.17

0.11

0.67

6.10

dPPAS2

-------------------------------------------------------------------------------------------------------------------------------------------------KEIGEEPDPEKLEAFLEEKGGNALSHLGFLGDKRFFYSSDGNALIQFAKVGQRLVVLGDPSGREDSFPLVIKEFLHAADQKGYLVIFYQIEREDMALYHDF-----GYRFFKLGEEAIVDLDTFTISGKKRAGLRAIYNRFEREGYTFHVEQ-PPFSREFLNELRQVSDEWLGRK-KEKGFSL--GFFQEDYLQKAPIAVLKSEEGEIVAFMNIMPMYREGEISIDLMRYSKKAPKGIMDALFIYLFQWGKEQGYTAFNMGMAPLSNVGTSFWT---ERLAAVIFNNVSYMYSFSGLRSFKEKYKPVWRGKYLAYRKNRSLPVTMILVTRLIGRRTK--

4v36A

0.17

0.11

0.67

4.43

PPAS

-------------------------------------------------------------------------------------------------------------------------------------------------KEIGEEPDPEKLEAFLEEKGGNALSHLGFLGDKRFFYSSDGNALIQFAKVGQRLVVLGDPSGREDSFPLVIKEFLHAADQKGYLVIFYQIEREDMALYHDF-----GYRFFKLGEEAIVDLDTFTISGKKRAGLRAIYNRFEREGYTFHVEQ-PPFSREFLNELRQVSDEWLGRKK-EKGFSL--GFFQEDYLQKAPIAVLKSEEGEIVAFMNIMPMYREGEISIDLMRYSKKAPKGIMDALFIYLFQWGKEQGYTAFNMGMAPLSNVGT---SFWTERLAAVIFNNVSYMYSFSGLRSFKEKYKPVWRGKYLAYRKNRSLPVTMILVTRLIGRRTK--

4v36A

0.17

0.11

0.67

7.20

Env-PPAS

-------------------------------------------------------------------------------------------------------------------------------------------------KEIGEEPDPEKLEAFLEEKGGNALSHLGFLGDKRFFYSSDGNALIQFAKVGQRLVVLGDPSGREDSFPLVIKEFLHAADQKGYLVIFYQIEREDMALYHDF-----GYRFFKLGEEAIVDLDTFTISGKKRAGLRAIYNRFEREGYTFHVEQ-PPFSREFLNELRQVSDEWLGRKK-EKGFSL--GFFQEDYLQKAPIAVLKSEEGEIVAFMNIMPMYREGEISIDLMRYSKKAPKGIMDALFIYLFQWGKEQGYTAFNMGMAPLSNVGT---SFWTERLAAVIFNNVSYMYSFSGLRSFKEKYKPVWRGKYLAYRKNRSLPVTMILVTRLIGRRTK--

4v34A

0.22

0.14

0.64

1.90

MUSTER

----------------------------------------------------------------------------------------------------------------------------------------------PPAIREPNAEELQRAARIIRHS-DQPDGGLALTGDKALLFHESDDAFLYAR--RGRSIALYDPIGP-AQRAELIWQFRDLCDLHHARPVFYQVRAENLPFY------DIGLTALKLGEEARVDLLRFDLENAAAKDLRYTWNRGQRDGLALEFHEPGQAP---LDELKAISDAW--------GFSL---GRFTPAYLNFFRIAIVRHQGKPVAFANLLETDSRELASLDLRVH-PDAPKL--TEFLLGLILHYKAQGHARFSLGV-PLAGLQPRRGAPLTQRLGALVFRRGEQFYNFQGLRRFKDKFQPDWEPRYLAVPAGLDPLVALADTAALIAGGLT--

4v34A

0.20

0.13

0.65

2.17

dPPAS

-----------------------------------------------------------------------------------------------------------------------------------------------PPAIREPNAEELQRAARIIRHSDQPDGGLALTGDKALLFHESDDAFLYAR--RGRSIALYDPIGP-AQRAELIWQFRDLCDLHHARPVFYQVRAENLPFY------DIGLTALKLGEEARVDLLRFDLENAAAKDLRYTWNRGQRDGLALEFHEPGQAP---LDELKAISDAWKGFS----------LGRFTPAYLNFFRIAIVRHQGKPVAFANLLETDSRELASLDLRVH-PDAPKL--TEFLLGLILHYKAQGHARFSLGV-PLAGLQPRRGAPLTQRLGALVFRRGEQFYNFQGLRRFKDKFQPDWEPRYLAVPAGLDPLVALADTAALIAGGLT--

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.36

Estimated TM-score = 0.55±0.15

Estimated RMSD = 10.4±4.6Å

Download Model 2

C-score=-0.26

Download Model 3

C-score=-0.48

Download Model 4

C-score=-0.70

Download Model 5

C-score=-0.71

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4v36A	0.661	1.06	0.166	0.671
2	4v34A	0.575	2.77	0.222	0.628
3	2hqyA	0.495	3.27	0.084	0.564
4	1xf8A	0.472	4.28	0.106	0.579
5	1lrzA	0.461	4.41	0.087	0.572
6	4zm6A	0.444	5.68	0.062	0.610
7	2c27A	0.411	4.71	0.093	0.529
8	3h5zA	0.407	6.08	0.063	0.585
9	4cavA	0.407	6.09	0.081	0.581
10	2p6eC	0.405	6.34	0.087	0.587

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.09	5	3dddA	COA	Rep, Mult	205,206,207,212,213,236,238,239,241,242,243,245
2	0.05	3	1q5yA	NI	Rep, Mult	283,287
3	0.04	2	1p0hA	COA	Rep, Mult	316,317,372,373,374,375,381,382,384,385
4	0.04	2	1iykB	MYA	Rep, Mult	201,211,212,213,216,220,223,224,229,231,233,234,238,460
5	0.02	1	4dz4A	UNK	Rep, Mult	371,382
6	0.02	1	3vq2A	LP5	Rep, Mult	202,457
7	0.02	1	1uf3A	CA	Rep, Mult	227,261
8	0.02	1	4x64P	MG	Rep, Mult	258,259
9	0.02	1	2axtK	CA	Rep, Mult	204,208
10	0.02	1	1vf5S	III	Rep, Mult	64,65
11	0.02	1	3p6lA	PO4	Rep, Mult	318,319,352
12	0.02	1	3zvmA	MG	Rep, Mult	366,371
13	0.02	1	1fbmD	RTL	Rep, Mult	54,61
14	0.02	1	4v35A	CA	Rep, Mult	220,230,461
15	0.02	1	1nx0B	III	Rep, Mult	383,387
16	0.02	1	4v36A	LYN	Rep, Mult	316,324,330,371,372,373,374
17	0.02	1	1bjpA	OXP	Rep, Mult	269,280
18	0.02	1	1bjyA	CTC	Rep, Mult	309,313
19	0.02	1	3lw5K	CLA	Rep, Mult	53,57
20	0.02	1	2hv8B	2ME	Rep, Mult	277,280

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2fh7A	0.310	7.75	0.050	0.539	3.1.3.48	NA
2	0.060	3cdjA	0.310	6.84	0.040	0.485	2.7.7.8	NA
3	0.060	3hz3A	0.338	7.57	0.023	0.570	2.4.1.5	158,162
4	0.060	2zxqA	0.318	7.77	0.028	0.558	3.2.1.97	182,189,204
5	0.060	1mx9D	0.314	6.94	0.039	0.498	3.1.1.1	NA
6	0.060	1p4nA	0.472	4.24	0.106	0.576	2.3.2.10	443,451
7	0.060	1cqzB	0.330	7.10	0.060	0.535	3.3.2.10,3.3.2.3	NA
8	0.060	3h5zA	0.407	6.08	0.063	0.585	2.3.1.97	NA
9	0.060	1k4yA	0.289	6.67	0.070	0.438	3.1.1.1	NA
10	0.060	2pffB	0.360	7.74	0.039	0.620	2.3.1.86	241
11	0.060	1e3pA	0.324	7.25	0.066	0.529	2.7.7.8	NA
12	0.060	1b41A	0.307	7.22	0.039	0.498	3.1.1.7	NA
13	0.060	1br2A	0.301	7.45	0.044	0.498	3.6.1.32	203
14	0.060	2et6A	0.315	7.75	0.047	0.539	1.1.1.-	NA
15	0.060	1nmtA	0.399	6.11	0.068	0.572	2.3.1.97	206,234,255
16	0.060	1lfwA	0.315	6.90	0.046	0.502	3.4.13.3	NA
17	0.060	3ecqB	0.324	8.00	0.030	0.572	3.2.1.97	169,329
18	0.060	2dqmA	0.319	7.20	0.033	0.514	3.4.11.2	NA
19	0.060	1vj5A	0.327	7.31	0.041	0.545	3.3.2.10,3.3.2.3	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.20	0.591	2.76	0.21	0.64	4v34A	GO:0016020 GO:0016021
1	0.16	0.661	1.06	0.17	0.67	4v36A	GO:0005886 GO:0006629 GO:0016020 GO:0016021 GO:0016740 GO:0046677 GO:0050071
2	0.07	0.471	4.33	0.12	0.58	4ii9A	GO:0008360 GO:0009252 GO:0016740 GO:0016746 GO:0016755 GO:0047206 GO:0071555
3	0.07	0.461	4.41	0.09	0.57	1lrzA	GO:0000166 GO:0005737 GO:0008360 GO:0009252 GO:0016740 GO:0016746 GO:0016755 GO:0071555
4	0.06	0.233	6.01	0.03	0.33	1umyD	GO:0005737 GO:0005829 GO:0006479 GO:0006579 GO:0008168 GO:0008270 GO:0008898 GO:0009086 GO:0010243 GO:0016740 GO:0032259 GO:0032403 GO:0043234 GO:0046872 GO:0047150
5	0.06	0.231	2.88	0.07	0.26	3e0kA	GO:0004042 GO:0005737 GO:0006526 GO:0008080 GO:0008652 GO:0016740 GO:0016746
6	0.06	0.232	7.75	0.03	0.39	5a9yA	GO:0000166 GO:0003924 GO:0005525 GO:0005829 GO:0009408 GO:0009409
7	0.06	0.206	7.37	0.04	0.34	5b04I	GO:0002183 GO:0003743 GO:0005085 GO:0005829 GO:0005851 GO:0006412 GO:0006413 GO:0009058 GO:0016779 GO:0043547
8	0.06	0.220	6.25	0.04	0.32	1f05A	GO:0003824 GO:0004801 GO:0005634 GO:0005737 GO:0005829 GO:0005975 GO:0005999 GO:0006002 GO:0006098 GO:0009052 GO:0016740 GO:0019682 GO:0030246 GO:0043231 GO:0048029 GO:0070062
9	0.06	0.182	6.24	0.03	0.27	5hoqB	GO:0008171 GO:0032259
10	0.06	0.191	6.15	0.05	0.28	4ikhA	GO:0004364 GO:0006749 GO:0016740
11	0.06	0.204	6.07	0.05	0.30	3e3xA	GO:0000166 GO:0003924 GO:0005525
12	0.06	0.169	5.55	0.04	0.23	1akeA	GO:0000166 GO:0000287 GO:0004017 GO:0005524 GO:0005737 GO:0005829 GO:0006139 GO:0006172 GO:0006412 GO:0008654 GO:0009165 GO:0015951 GO:0016208 GO:0016301 GO:0016310 GO:0016740 GO:0016776 GO:0019205 GO:0032774 GO:0044209 GO:0046939
13	0.06	0.116	5.74	0.02	0.16	5fmf2	GO:0000993 GO:0001012 GO:0001139 GO:0001193 GO:0003676 GO:0003677 GO:0005634 GO:0006351 GO:0006355 GO:0006357 GO:0006362 GO:0006367 GO:0006368 GO:0006383 GO:0008270 GO:0031440 GO:0031564 GO:0032784 GO:0032968 GO:0042797 GO:0045899 GO:0046872 GO:0051123
14	0.06	0.126	5.26	0.03	0.17	2ehrA	GO:0005737 GO:0005886 GO:0005923 GO:0016020 GO:0016324 GO:0030054 GO:0035556 GO:0043234 GO:0045177 GO:0048471 GO:0070062 GO:0070830
15	0.06	0.111	4.78	0.02	0.14	1x4rA	GO:0003950 GO:0005634 GO:0005737 GO:0006351 GO:0006355 GO:0016020 GO:0016740 GO:0016757
16	0.06	0.134	4.99	0.03	0.17	2z8hA	GO:0000083 GO:0000117 GO:0000122 GO:0000980 GO:0001078 GO:0001205 GO:0001206 GO:0003677 GO:0003700 GO:0004842 GO:0005634 GO:0005737 GO:0005829 GO:0006281 GO:0006351 GO:0006355 GO:0006366 GO:0016567 GO:0031463 GO:0043565 GO:0045944 GO:0061418
17	0.06	0.084	3.53	0.07	0.10	2jvgA	GO:0005576 GO:0009405 GO:0019864 GO:0019865
18	0.06	0.108	4.24	0.01	0.13	2lchA

Consensus prediction of GO terms

Molecular Function	GO:0016755
GO-Score	0.32
Biological Processes	GO:0044710	GO:0042221
GO-Score	0.32	0.32
Cellular Component	GO:0016021	GO:0071944
GO-Score	0.33	0.32

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.