I-TASSER results

I-TASSER results for job id Rv1501

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (273 residues)
MIPVKVENNTSLDQVQDALNCVGYAVVEDVLDEASLAATRDRMYRVQERILTEIGKERLA
RAGELGVLRLMMKYDPHFFTFLEIPEVLSIVDRVLSETAILHLQNGFILPSFPPFSTPDV
FQNAFHQDFPRVLSGYIASVNIMFAIDPFTRDTGATLVVPGSHQRIEKPDHTYLARNAVP
VQCAAGSLFVFDSTLWHAAGRNTSGKDRLAINHQFTRSFFKQQIDYVRALGDAVVLEQPA
RTQQLLGWYSRVVTNLDEYYQPPDKRLYRKGQG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MIPVKVENNTSLDQVQDALNCVGYAVVEDVLDEASLAATRDRMYRVQERILTEIGKERLARAGELGVLRLMMKYDPHFFTFLEIPEVLSIVDRVLSETAILHLQNGFILPSFPPFSTPDVFQNAFHQDFPRVLSGYIASVNIMFAIDPFTRDTGATLVVPGSHQRIEKPDHTYLARNAVPVQCAAGSLFVFDSTLWHAAGRNTSGKDRLAINHQFTRSFFKQQIDYVRALGDAVVLEQPARTQQLLGWYSRVVTNLDEYYQPPDKRLYRKGQG
Prediction	CCCCCCCCCCCHHHHHHHHHHCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHCHHHHHHHHHHHCCCCEEEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCCCCCCCEEEHCCCCCCCCCCCCCCCCCCEEEEHCCCCEEEEHCCCCCHCCCCCCCCCCEEEEEEEEHCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCC
Conf.Score	987657999999999999987588996899999999999999999999655667766566788644564456659899999979799999999989985898765566468888888988888886678888889875799999955799877878998786789999887877676688666998799995996456889999998689999995554577666644699889886999999998988788877766778856656666789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MIPVKVENNTSLDQVQDALNCVGYAVVEDVLDEASLAATRDRMYRVQERILTEIGKERLARAGELGVLRLMMKYDPHFFTFLEIPEVLSIVDRVLSETAILHLQNGFILPSFPPFSTPDVFQNAFHQDFPRVLSGYIASVNIMFAIDPFTRDTGATLVVPGSHQRIEKPDHTYLARNAVPVQCAAGSLFVFDSTLWHAAGRNTSGKDRLAINHQFTRSFFKQQIDYVRALGDAVVLEQPARTQQLLGWYSRVVTNLDEYYQPPDKRLYRKGQG
Prediction	744341455541440252156321000430036621630261035015524763445425455433103100431400130042430140032003450201112210234343353345442200000212334322100000000313541000100110132432245434442102030430000000020032034132743020000000222043432232303462065025302420203233144154224223431345568
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCHHHHHHHHHHCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHCHHHHHHHHHHHCCCCEEEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHCCCCCCCCCEEEHCCCCCCCCCCCCCCCCCCEEEEHCCCCEEEEHCCCCCHCCCCCCCCCCEEEEEEEEHCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCCCC
MIPVKVENNTSLDQVQDALNCVGYAVVEDVLDEASLAATRDRMYRVQERILTEIGKERLARAGELGVLRLMMKYDPHFFTFLEIPEVLSIVDRVLSETAILHLQNGFILPSFPPFSTPDVFQNAFHQDFPRVLSGYIASVNIMFAIDPFTRDTGATLVVPGSHQRIEKPDHTYLARNAVPVQCAAGSLFVFDSTLWHAAGRNTSGKDRLAINHQFTRSFFKQQIDYVRALGDAVVLEQPARTQQLLGWYSRVVTNLDEYYQPPDKRLYRKGQG

4y5sA

0.18

0.97

2.57

MUSTER

PQLQRLAADADVDRMCRLLEEDGAFILKGLLPFDVVESFNRELDVQMAIPPPKGERLLAKYPPHFKYVPNVATTCPTFRNTVINPVIHAICEAYFQRTGDYWLSAAFLR-----EIESGMPAQPFHRDDATHLEAPPVSLSVIFPLTEFTEENGATEVILGSHRWTEV--GTPERDQAVLATMDPGDVLIVRQRVVHAGGGNRTGKPRRVVLAYFNSVQLTPFETY-RTMPREMVESMTVLGQRMLGWRTMKPSDPNIVGINLENVLQLKAAD

4naoA

0.15

0.14

0.96

3.57

dPPAS

-GTKRFSIQSDPVEIHRAIVEDGVAIIEGFLTPEQVQKLNKDVDAPLKADREQFWLA-DFIPDHVARVHNLVDFSHCFRHEILNHELLHKICRLTFGDYWLGYGAVIEN-------GPGTTEQKWHRDQPRYPLAPEGMLNFFTALTDFDAETGKTQYILGSNKRVELGEPDADH-PIEYVGLKPGDTTIVSGKITHRGSDNRSDKMRRAMPIMIIPSILTPFDA-TCHLSRELVETMTPLAQKMICRRSVMIPAKCVNMREAGEQIGLKSNQ

4naoA

0.15

0.14

0.96

4.47

wdPPAS

-GTKRFSIQSDPVEIHRAIVEDGVAIIEGFLTPEQVQKLNKDVDAPLKADREQ-FWLADFIPDHVARVHNLVDFSHCFRHEILNHELLHKICRLTFGDYWLGYGAVIENG-------PGTTEQKWHRDQPRYPLVPEGMLNFFTALTDFDAETGKTQYILGSNKRVELGEPD-ADHPIEYVGLKPGDTTIVSGKITHRGSDNRSDKMRRAMPIMIIPSILTPFDA-TCHLSRELVETMTPLAQKMICRRSAKTGIWCVNMREAGEQIGKSNQR

4y5sA

0.18

0.96

2.97

wMUSTER

L--QRLAADADVDRMCRLLEEDGAFILKGLLPFDVVESFNRELDVQMAIPPPKGERLLAKYPPHFKYVPNVATTCPTFRNTVINPVIHAICEAYFQRTGDYWLSAAFLR-----EIESGMPAQPFHRDDATHLEAPPVSLSVIFPLTEFTEENGATEVILGSHRWTEV--GTPERDQAVLATMDPGDVLIVRQRVVHAGGGNRTGKPRRVVLAYFNSVQLTPFETY-RTMPREMVESMTVLGQRMLGWRTMKPSDPNIVGINLENVLQLKAAD

4naoA

0.15

0.14

0.96

4.65

wPPAS

-GTKRFSIQSDPVEIHRAIVEDGVAIIEGFLTPEQVQKLNKDVDAPLKADREQFWLAD-FIPDHVARVHNLVDFSHCFRHEILNHELLHKICRLTFGDYWLGYGAVIENG-------PGTTEQKWHRDQPRYPLVPEGMLNFFTALTDFDAETGKTQYILGSNKRVELGEPDAD-HPIEYVGLKPGDTTIVSGKITHRGSDNRSDKMRRAMPIMIIPSILTPFDAT-CHLSRELVETMTPLAQKMICRRSAKTGIWCVNMREAGEQI-KSNQR

4y5sA

0.18

0.96

6.36

dPPAS2

LQ--RLAADADVDRMCRLLEEDGAFILKGLLPFDVVESFNRELDVQMAIPPPKGERLLAKYPPHFKYVPNVATTCPTFRNTLINPVIHAICEAYFQRTGDYWLSAAFLR-----EIESGMPAQPFHRDDATHLEAPPVSLSVIFPLTEFTEENGATEVILGSHRWTEVG--TPERDQAVLATMDPGDVLIVRQRVVHAGGGNRTGKPRRVVLAYFNSVQLTPFET-YRTMPREMVESMTVLGQRMLGWRTMKPSDPNIVGINLENVLQLKAAD

4naoA

0.15

0.14

0.96

4.06

PPAS

-GTKRFSIQSDPVEIHRAIVEDGVAIIEGFLTPEQVQKLNKDVDAPLKADREQFWLAD-FIPDHVARVHNLVDFSHCFRHEILNHELLHKICRLTFGDYWLGYGAVIENG-------PGTTEQKWHRDQPRYPLAPEGMLNFFTALTDFDAETGKTQYILGSNKRVELGEPDAD-HPIEYVGLKPGDTTIVSGKITHRGSDNRSDKMRRAMPIMIIPSILTPFDAT-CHLSRELVETMTPLAQKMICRRSAKTGIWCVNMREAGEQIGKSNQR

4naoA

0.15

0.14

0.96

4.09

Env-PPAS

-GTKRFSIQSDPVEIHRAIVEDGVAIIEGFLTPEQVQKLNKDVDAPLKADREQ-FWLADFIPDHVARVHNLVDFSHCFRHEILNHELLHKICRLTFGDYWLGYGAVIENG-------PGTTEQKWHRDQPRYPLAPEGMLNFFTALTDFDAETGKTQYILGSNKRVELGEPDAD-HPIEYVGLKPGDTTIVSGKITHRGSDNRSDKMRRAMPIMIIPSILTPFDAT-CHLSRELVETMTPLAQKMICRRPAKTGIWCVNMREAGEQILKSNQR

4naoA

0.15

0.14

0.96

2.57

MUSTER

-GTKRFSIQSDPVEIHRAIVEDGVAIIEGFLTPEQVQKLNKDVDAPLKADREQFWLAD-FIPDHVARVHNLVDFSHCFRHEILNHELLHKICRLTFGDYWLGYGAVIEN-------GPGTTEQKWHRDQPRYPDAPEGMLNFFTALTDFDAETGKTQYILGSNKRVELGEPDAD-HPIEYVGLKPGDTTIVSGKITHRGSDNRSDKMRRAMPIMIIPSILTPFDAT-CHLSRELVETMTPLAQKMICRRSVMIPIWCVNMREAGEQIGKSNQR

4y5sA

0.18

0.97

3.56

dPPAS

PQLQRLAADADVDRMCRLLEEDGAFILKGLLPFDVVESFNRELDVQMAIPPPKGERLLAKYPPHFKYVPNVATTCPTFRNTLINPVIHAICEAYFQRTGDYWLSAAFLR-----EIESGMPAQPFHRDDATHLEAPPVSLSVIFPLTEFTEENGATEVILGSHRWTEVG--TPERDQAVLATMDPGDVLIVRQRVVHAGGGNRTGKPRRVVLAYFNSVQLTPFET-YRTMPREMVESMTVLGQRMLGWRTMKPSDPNIVGINLENVLQLKAAD

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 1 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.76

Estimated TM-score = 0.82±0.09

Estimated RMSD = 4.4±2.9Å

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4y5sA	0.884	2.02	0.163	0.963
2	4naoA	0.877	2.26	0.137	0.960
3	5dapA	0.872	2.27	0.152	0.963
4	4xbzA	0.735	3.67	0.142	0.897
5	2csgA	0.702	3.67	0.087	0.883
6	4rgkA	0.687	3.73	0.091	0.872
7	2opwA	0.664	3.38	0.150	0.806
8	4mhrA	0.660	3.69	0.135	0.802
9	5ep9A	0.657	3.80	0.144	0.809
10	2fcuA	0.654	4.30	0.154	0.843

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.32	28	4mhuA	FE	Rep, Mult	126,128,197
2	0.32	27	5equD	AKG	Rep, Mult	107,109,123,126,128,143,156,197,199,208,212
3	0.02	2	2fctA	DSU	Rep, Mult	95,96,136,137,163,192,194

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.088	2a1xA	0.623	3.59	0.164	0.766	1.14.11.18	197
2	0.066	1unbA	0.557	3.60	0.102	0.700	1.14.20.1	NA
3	0.060	1ep0A	0.372	4.04	0.074	0.483	5.1.3.13	207
4	0.060	1upiA	0.375	3.90	0.065	0.487	5.1.3.13	NA
5	0.060	1e5rB	0.439	3.26	0.076	0.531	1.14.11.28	142,149
6	0.060	1bk0A	0.561	3.68	0.072	0.700	1.21.3.1	NA
7	0.060	3h09B	0.376	6.67	0.043	0.696	3.4.21.72	159
8	0.060	1ywkA	0.391	4.30	0.104	0.516	5.3.1.17	109,123
9	0.060	2ilmA	0.544	3.84	0.070	0.689	1.14.11.16	NA
10	0.060	1t3tA	0.411	6.35	0.050	0.736	6.3.5.3	NA
11	0.060	3elnA	0.386	4.29	0.032	0.502	1.13.11.20	NA
12	0.060	1sg3A	0.407	5.57	0.060	0.641	3.5.3.4	NA
13	0.060	1aorB	0.357	6.58	0.096	0.630	1.2.7.5	NA
14	0.060	1rtvA	0.373	4.21	0.067	0.494	5.1.3.13	NA
15	0.060	2yu1A	0.581	4.86	0.107	0.802	1.14.11.27	12,19
16	0.060	2ztgA	0.398	5.83	0.049	0.659	6.1.1.7	39,210
17	0.060	1gw0A	0.382	5.81	0.049	0.612	1.10.3.2	NA
18	0.060	1wltA	0.374	3.70	0.070	0.476	5.1.3.13	163
19	0.060	1gp6A	0.558	3.53	0.094	0.700	1.14.11.19	NA
20	0.060	1xruB	0.396	4.60	0.075	0.538	5.3.1.17	193
21	0.060	2i4nB	0.378	5.82	0.033	0.608	6.1.1.15	251

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.36	0.884	2.02	0.16	0.96	4y5sA	GO:0016491 GO:0051213 GO:0055114 GO:1902181
1	0.34	0.872	2.27	0.15	0.96	5dapA	GO:0051213 GO:0055114
2	0.33	0.877	2.26	0.14	0.96	4naoA	GO:0046872
3	0.28	0.623	3.59	0.16	0.77	2a1xA	GO:0001561 GO:0003824 GO:0005739 GO:0005777 GO:0005782 GO:0006631 GO:0006720 GO:0016491 GO:0031418 GO:0046872 GO:0048037 GO:0048244 GO:0051213 GO:0055114 GO:0097089
4	0.28	0.663	3.42	0.15	0.81	3obzA	GO:0016491 GO:0046872 GO:0051213 GO:0055114
5	0.24	0.658	3.75	0.13	0.80	4q5oA	GO:0016706 GO:0046872 GO:0055114
6	0.19	0.654	3.60	0.13	0.80	4nmiA	GO:0005506 GO:0016491 GO:0016706 GO:0019491 GO:0042400 GO:0046872 GO:0051213 GO:0055114
7	0.06	0.321	5.78	0.06	0.52	3qvfA	GO:0000166 GO:0005524 GO:0016301 GO:0016310 GO:0016740
8	0.06	0.334	5.44	0.04	0.50	4qqaA	GO:0009405 GO:0015485
9	0.06	0.335	6.37	0.05	0.58	4n5fA	GO:0003995 GO:0008152 GO:0016491 GO:0016627 GO:0050660 GO:0055114
10	0.06	0.331	5.94	0.04	0.55	3wnkA	GO:0016740 GO:0016757 GO:0030246
11	0.06	0.315	6.16	0.05	0.52	3bjxA	GO:0019120
12	0.06	0.323	6.40	0.04	0.56	3hlfA	GO:0016740 GO:0016746 GO:0016787 GO:0017000 GO:0030639 GO:0050832
13	0.06	0.302	6.35	0.04	0.53	3ip4B	GO:0000166 GO:0005524 GO:0006412 GO:0016874 GO:0016884 GO:0050567
14	0.06	0.287	6.45	0.05	0.52	5bxpA	GO:0004553 GO:0004563 GO:0005975 GO:0008152 GO:0016020 GO:0016021 GO:0016787 GO:0016798 GO:0047403
15	0.06	0.280	6.22	0.06	0.48	4hacA	GO:0000166 GO:0000287 GO:0004496 GO:0005524 GO:0005737 GO:0006629 GO:0008152 GO:0008299 GO:0016301 GO:0016310 GO:0016740 GO:0016773 GO:0019287
16	0.06	0.291	5.70	0.05	0.47	1x19A	GO:0008168 GO:0008171 GO:0016740 GO:0032259 GO:0046872 GO:0051536 GO:0051539
17	0.06	0.294	6.60	0.03	0.53	3wnbA	GO:0003746 GO:0006414
18	0.06	0.288	6.48	0.03	0.51	2oekA	GO:0000287 GO:0008652 GO:0009086 GO:0015977 GO:0016853 GO:0016984 GO:0019284 GO:0019509 GO:0043715 GO:0046872
19	0.06	0.300	4.90	0.03	0.43	2f17A	GO:0000166 GO:0004788 GO:0005524 GO:0006772 GO:0009229 GO:0016301 GO:0016310 GO:0016740 GO:0030975
20	0.06	0.287	6.97	0.05	0.55	3vehB	GO:0000287 GO:0004106 GO:0008909 GO:0009058 GO:0009697 GO:0016829 GO:0016833 GO:0016853 GO:0019540 GO:0043904 GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0046872	GO:0019842	GO:0031406	GO:0016706
GO-Score	0.65	0.56	0.56	0.56
Biological Processes	GO:0019395	GO:0009062	GO:1902181
GO-Score	0.56	0.56	0.36
Cellular Component	GO:0031907	GO:0044439
GO-Score	0.56	0.56

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.