I-TASSER results

I-TASSER results for job id Rv1462

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (397 residues)
MTAPGLTAAVEGIAHNKGELFASFDVDAFEVPHGRDEIWRFTPLRRLRGLHDGSARATGS
ATITVSERPGVYTQTVRRGDPRLGEGGVPTDRVAAQAFSSFNSATLVTVERDTQVVEPVG
ITVTGPGEGAVAYGHLQVRIEELGEAVVVIDHRGGGTYADNVEFVVDDAARLTAVWIADW
ADNTVHLSAHHARIGKDAVLRHVTVMLGGDVVRMSAGVRFCGAGGDAELLGLYFADDGQH
LESRLLVDHAHPDCKSNVLYKGALQGDPASSLPDAHTVWVGDVLIRAQATGTDTFEVNRN
LVLTDGARADSVPNLEIETGEIVGAGHASATGRFDDEQLFYLRSRGIPEAQARRLVVRGF
FGEIIAKIAVPEVRERLTAAIEHELEITESTEKTTVS

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTAPGLTAAVEGIAHNKGELFASFDVDAFEVPHGRDEIWRFTPLRRLRGLHDGSARATGSATITVSERPGVYTQTVRRGDPRLGEGGVPTDRVAAQAFSSFNSATLVTVERDTQVVEPVGITVTGPGEGAVAYGHLQVRIEELGEAVVVIDHRGGGTYADNVEFVVDDAARLTAVWIADWADNTVHLSAHHARIGKDAVLRHVTVMLGGDVVRMSAGVRFCGAGGDAELLGLYFADDGQHLESRLLVDHAHPDCKSNVLYKGALQGDPASSLPDAHTVWVGDVLIRAQATGTDTFEVNRNLVLTDGARADSVPNLEIETGEIVGAGHASATGRFDDEQLFYLRSRGIPEAQARRLVVRGFFGEIIAKIAVPEVRERLTAAIEHELEITESTEKTTVS
Prediction	CCCCCCCCCCCCHHHCHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEHCCCCCEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEHCCCCEHCCCEEEEEEHCCCCCCEEEEEEEEHCCCCEEEEEEEEHCCCCEEEEEEEEHCCCCEEEEEEEHCCCCCEEEEHCEEEEHCCCCEEEEEEEEHCCCEEEEEEEEEEHCCCCEEEEEEEEEHCCCEEEHCCEEEEEHCCCCEEEEEEEEEHCCCCCCCCCCCEEEEEEEEEHCCCCCCCCCCEHCCEEEHCCCCEEHCCCEEEEHCCCCCEEEEEEHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
Conf.Score	9865567776656655889987678677899998886445689889886577777777765667654688435666655566654789875788999987489699998898565887899999868987555689999879985999987656775566899998799689999997579987998755999879978999999969975788558999869958999999997698799755799986888588898876766988777788668999999977988778754344758858998677565567766975455565545689988999999879999999999999999999997899999999999999999965555555679
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTAPGLTAAVEGIAHNKGELFASFDVDAFEVPHGRDEIWRFTPLRRLRGLHDGSARATGSATITVSERPGVYTQTVRRGDPRLGEGGVPTDRVAAQAFSSFNSATLVTVERDTQVVEPVGITVTGPGEGAVAYGHLQVRIEELGEAVVVIDHRGGGTYADNVEFVVDDAARLTAVWIADWADNTVHLSAHHARIGKDAVLRHVTVMLGGDVVRMSAGVRFCGAGGDAELLGLYFADDGQHLESRLLVDHAHPDCKSNVLYKGALQGDPASSLPDAHTVWVGDVLIRAQATGTDTFEVNRNLVLTDGARADSVPNLEIETGEIVGAGHASATGRFDDEQLFYLRSRGIPEAQARRLVVRGFFGEIIAKIAVPEVRERLTAAIEHELEITESTEKTTVS
Prediction	6537524443531453244014214076161134340302103053047154441436423414044154022620452243026233462200000101011000010266050630010001031641211210001015404010002144431100000010256040210101223530110121303025604021110101032021202020206504020100000364121112020202054040312010002244444364030002020203550640413330300001670422010101042633040201011142365101001124143730330003000330075051750252035105520541454654448
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCHHHCHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEHCCCCCEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEHCCCCEHCCCEEEEEEHCCCCCCEEEEEEEEHCCCCEEEEEEEEHCCCCEEEEEEEEHCCCCEEEEEEEHCCCCCEEEEHCEEEEHCCCCEEEEEEEEHCCCEEEEEEEEEEHCCCCEEEEEEEEEHCCCEEEHCCEEEEEHCCCCEEEEEEEEEHCCCCCCCCCCCEEEEEEEEEHCCCCCCCCCCEHCCEEEHCCCCEEHCCCEEEEHCCCCCEEEEEEHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
MTAPGLTAAVEGIAHNKGELFASFDVDAFEVPHGRDEIWRFTPLRRLRGLHDGSARATGSATITVSERPGVYTQTVRRGDPRLGEGGVPTDRVAAQAFSSFNSATLVTVERDTQVVEPVGITVTGPGEGAVAYGHLQVRIEELGEAVVVIDHRGGGTYADNVEFVVDDAARLTAVWIADWADNTVHLSAHHARIGKDAVLRHVTVMLGGDVVRMSAGVRFCGAGGDAELLGLYFADDGQHLESRLLVDHAHPDCKSNVLYKGALQGDPASSLPDAHTVWVGDVLIRAQATGTDTFEVNRNLVLTDGARADSVPNLEIETGEIVGAGHASATGRFDDEQLFYLRSRGIPEAQARRLVVRGFFGEIIAKIAVPEVRERLTAAIEHELEITESTEKTTVS

2zu0A

0.22

0.21

0.96

3.36

MUSTER

LQHHLFEAEGTKRSPQAQQHLQQ--LLRTGLPTRKHENWKYTPLEGLINSQFVSIAGETLDSVRLVFVDGRYVPALSDNDDRQGLPAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGEVNTAHYRHHLDLAEGAEATVIEHFVSLNFTGARFTINVAANAHLQHIKLAFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDK-------GRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVDTKPQLEIYADD-VKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYAFAAELTEALRDEGLKQQVLARIGQRLPGGAR-------

2zu0A

0.21

0.20

0.96

7.97

dPPAS

QWHHLFEAEGTKRSPQAQQHLQQ--LLRTGLPTRKHENWKYTPLEGLINSQFVSIAGETLDSVRLVFVDGRYVPALSDATEGSGPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGVAGEAHYRHHLDLAEGAEATVIEHFVSLNFTGARFTINVAANAHLQHIKLAFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDK-------GRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVDTKPQLEIYADD-VKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYAFAAELTEALRDEGLKQQVLARIGQRLPGGAR-------

2zu0A

0.21

0.20

0.95

9.23

wdPPAS

HHL--FEAEGTKRSPQAQQHLQQ--LLRTGLPTRKHENWKYTPLEGLINSQFVSIAGETLDSVRLVFVDGRYVPALSDATEGSGPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGVAGEAHYRHHLDLAEGAEATVIEHFVSLNFTGARFTINVAANAHLQHIKLAFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDK-------GRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVDTKPQLEIYADD-VKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYAFAAELTEALRDEGLKQQVLARIGQRLPGGAR-------

2zu0A

0.22

0.21

0.96

4.10

wMUSTER

LQHHLFEAEGTKRSPQAQQHLQQ--LLRTGLPTRKHENWKYTPLEGLINSQFVSIAGETLDSVRLVFVDGRYVPALSDADDRQGLPAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGEVNTAHYRHHLDLAEGAEATVIEHFVSLNFTGARFTINVAANAHLQHIKLAFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDK-------GRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVDTKPQLEIYADD-VKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYAFAAELTEALRDEGLKQQVLARIGQRLPGGAR-------

2zu0A

0.22

0.21

0.95

6.33

wPPAS

HHLFE--AEGTKRSPQAQQHLQQ--LLRTGLPTRKHENWKYTPLEGLINSQFVSIAGETLDSVRLVFVDGRYVPALSDADDRQGLPDAQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGEVNTAHYRHHLDLAEGAEATVIEHFVSLNFTGARFTINVAANAHLQHIKLAFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDK-------GRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVDTKPQLEIYADD-VKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYAFAAELTEALRDEGLKQQVLARIGQRLPGGAR-------

2zu0A

0.21

0.20

0.95

13.39

dPPAS2

L----FEAEGTKRSPQAQQHLQQ--LLRTGLPTRKHENWKYTPLEGLINSQFVSIAGETLDSVRLVFVDGRYVPALSDATEGSGPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGVAGEAHYRHHLDLAEGAEATVIEHFVSLNFTGARFTINVAANAHLQHIKLAFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDK-------GRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVDTKPQLEIYADD-VKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYAFAAELTEALRDEGLKQQVLARIGQRLPGGAR-------

2zu0A

0.22

0.21

0.96

5.53

PPAS

LQHHLFEAEGTKRSPQAQQHLQQ--LLRTGLPTRKHENWKYTPLEGLINSQFVSIAGETLDSVRLVFVDGRYVPALSDADDRQGLPDAQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGVAGEAHYRHHLDLAEGAEATVIEHFVSLNFTGARFTINVAANAHLQHIKLAFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDK-------GRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVDTKPQLEIYADD-VKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYAFAAELTEALRDEGLKQQVLARIGQRLPGGAR-------

2zu0A

0.21

0.20

0.96

8.10

Env-PPAS

LQHHLFEAEGTKRSPQAQQHLQQ--LLRTGLPTRKHENWKYTPLEGLINSQFVSIAGETLDSVRLVFVDGRYVPALSDATEGSGPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGEVNTAHYRHHLDLAEGAEATVIEHFVSLNFTGARFTINVAANAHLQHIKLAFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMPVKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDK-------GRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVDTKPQLEIYADD-VKCSHGATVGRIDDEQIFYLRSRGINQQDAQQMIIYAFAAELTEALRDEGLKQQVLARIGQRLPGGAR-------

5awfA

0.16

0.15

0.91

3.17

MUSTER

INE-AKRNEPEWMLEFRLNAYRA--WLEMEEPHWLK--------------AHEKLAEQGIIFCSFGEAIHDHPELVRKYL---GTVVPGNNFFAALNAAVASDGTFIYVPKGVRCPMELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAYQLHAAVVEVIIHKNAEVKYSTVQNWTGGILNFVTKRALCGENSKMSWTQSETGSAITWKYPSCILRGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGH-------SQNSYRGLVKIMPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNS-AQLEHEATTSRIGEDQLFYCLQRGISEEDAISMIVNGFCKDVFSELP-LEFAVEAQKLLAISLEHSVG-------

5awfA

0.15

0.14

0.91

7.47

dPPAS

RAISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLK----------------AHEKLAEQGIIFCSFGEAIHDHPELVRKYLGT--VVPGNDNFFAALNAAVASDGTFIYVPKGVRCPMELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAYQLHAAVVEVIIHKNAEVKYSTVQNWTGGILNFVTKRALCGENSKMSWTQSETGSAITWKYPSCILRGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGH-------SQNSYRGLVKIMPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNS-AQLEHEATTSRIGEDQLFYCLQRGISEEDAISMIVNGFCKDVFSELP-LEFAVEAQKLLAISLEHSVG-------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.08

Estimated TM-score = 0.86±0.07

Estimated RMSD = 4.5±3.0Å

Download Model 2

C-score=-1.30

Download Model 3

C-score=0.82

Download Model 4

C-score=-2.30

Download Model 5

C-score=-3.32

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2zu0A	0.933	1.25	0.211	0.957
2	4dn7A	0.792	2.64	0.173	0.874
3	5awfA	0.775	3.16	0.163	0.872
4	3eqnA	0.525	4.75	0.060	0.690
5	4pewA	0.506	4.86	0.063	0.678
6	3vstA	0.493	6.30	0.063	0.763
7	4hwvA	0.467	5.78	0.063	0.678
8	1dboA	0.463	5.81	0.047	0.683
9	2vdcA	0.460	5.64	0.070	0.667
10	1ogmX	0.458	5.68	0.041	0.662

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.13	3	2zu0B	III	Rep, Mult	337,338,341,342,345,346,347,355,359
2	0.09	3	2vdcA	F3S	Rep, Mult	363,365,367,368,369,370,371,375
3	0.06	2	3etdC	B1T	Rep, Mult	372,376
4	0.06	2	3c23A	3AT	Rep, Mult	341,345
5	0.06	2	1qiyL	IPH	Rep, Mult	377,380
6	0.03	1	2uveA	NI	Rep, Mult	197,225,255
7	0.03	1	1vh40	III	Rep, Mult	37,40,41,287,302,304,305,306,308,309,310,311,312,313,314,315,316,317,318,319,320,321,323,325,326,327,328,329,330,331,332,333
8	0.03	1	3d5bG	MG	Rep, Mult	346,348
9	0.03	1	1rmgA	UUU	Rep, Mult	138,163,192,220
10	0.03	1	3wmnA	MQ8	Rep, Mult	381,385

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2z8gB	0.454	5.84	0.046	0.665	3.2.1.57	171,292
2	0.060	1qcxA	0.422	5.21	0.064	0.589	4.2.2.10	NA
3	0.060	1k5cA	0.416	4.81	0.049	0.557	3.2.1.15	157
4	0.060	1bheA	0.430	5.07	0.063	0.589	3.2.1.15	293
5	0.060	1qjvA	0.398	5.59	0.038	0.584	3.1.1.11	253
6	0.060	2vdcA	0.460	5.64	0.070	0.667	1.4.1.13	NA
7	0.060	1ogmX	0.458	5.68	0.041	0.662	3.2.1.11	210
8	0.060	2iq7A	0.418	4.84	0.037	0.559	3.2.1.15	NA
9	0.060	1llwA	0.458	5.82	0.064	0.678	1.4.7.1	NA
10	0.060	2qx3A	0.408	4.86	0.028	0.547	4.2.2.2	160,244
11	0.060	2pecA	0.418	4.98	0.065	0.557	4.2.2.2	NA
12	0.060	1ogoX	0.426	6.03	0.047	0.635	3.2.1.11	147,199,224
13	0.060	2vdcF	0.461	5.62	0.070	0.667	1.4.1.13	195
14	0.060	2ntqB	0.396	5.56	0.038	0.579	3.1.1.11	162
15	0.060	1ib4A	0.425	4.53	0.045	0.554	3.2.1.15	161
16	0.060	1wmrA	0.451	5.88	0.049	0.667	3.2.1.57	NA
17	0.060	1ia5A	0.417	4.86	0.026	0.559	3.2.1.15	197
18	0.060	3jurD	0.425	4.66	0.067	0.564	3.2.1.15	NA
19	0.060	1oocB	0.437	5.23	0.052	0.597	4.2.2.2	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.40	0.933	1.25	0.21	0.96	2zu0A	GO:0006979 GO:0016226
1	0.29	0.775	3.16	0.16	0.87	5awfA	GO:0016226 GO:0051539
2	0.25	0.810	2.64	0.17	0.89	4dn7A	GO:0005524 GO:0016226
3	0.08	0.435	5.73	0.03	0.62	1h80A	GO:0000272 GO:0005576 GO:0005615 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0033952 GO:0071555
4	0.07	0.525	4.75	0.06	0.69	3eqnA	GO:0004338 GO:0008152 GO:0016787 GO:0016798
5	0.07	0.512	4.83	0.08	0.68	4pexB	GO:0000272 GO:0004338 GO:0005576 GO:0005975 GO:0008152 GO:0009251 GO:0016787 GO:0016798
6	0.06	0.447	5.54	0.04	0.63	2uveA	GO:0004650 GO:0005975 GO:0008152 GO:0016787 GO:0016798
7	0.06	0.435	5.66	0.04	0.62	3lmwA	GO:0000272 GO:0005576 GO:0005615 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0033952 GO:0071555
8	0.06	0.409	5.19	0.07	0.56	3sucA	GO:0000166 GO:0005524 GO:0016032 GO:0019012 GO:0019062 GO:0046718 GO:0046872
9	0.06	0.313	6.84	0.05	0.53	2x6kA	GO:0000166 GO:0000421 GO:0005524 GO:0005769 GO:0006897 GO:0006914 GO:0009267 GO:0010506 GO:0016301 GO:0016303 GO:0016310 GO:0016322 GO:0016740 GO:0033227 GO:0034271 GO:0035004 GO:0035005 GO:0035032 GO:0035096 GO:0036092 GO:0045850 GO:0046854 GO:0046934 GO:0048015
10	0.06	0.270	7.01	0.07	0.46	2yhaA	GO:0003676
11	0.06	0.277	6.66	0.05	0.45	2q3oB	GO:0003824 GO:0003959 GO:0005777 GO:0006629 GO:0006631 GO:0006633 GO:0009620 GO:0009695 GO:0010181 GO:0010193 GO:0016491 GO:0016629 GO:0031408 GO:0055114
12	0.06	0.268	7.21	0.04	0.46	3gvjA	GO:0008152 GO:0016787 GO:0016798 GO:0016996
13	0.06	0.270	5.25	0.05	0.38	1jjfA	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0030246 GO:0031176 GO:0033905 GO:0045493
14	0.06	0.183	6.24	0.09	0.29	4n0vA	GO:0016740
15	0.06	0.202	5.63	0.04	0.29	4oigA	GO:0000166 GO:0001172 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0003968 GO:0004252 GO:0004386 GO:0004482 GO:0004483 GO:0005198 GO:0005524 GO:0005576 GO:0006351 GO:0006355 GO:0006370 GO:0006397 GO:0006508 GO:0008026 GO:0008152 GO:0008168 GO:0008233 GO:0008236 GO:0016020 GO:0016021 GO:0016032 GO:0016070 GO:0016740 GO:0016779 GO:0016787 GO:0016817 GO:0017111 GO:0019012 GO:0019028 GO:0019031 GO:0019058 GO:0019062 GO:0019079 GO:0030683 GO:0032259 GO:0033644 GO:0036265 GO:0039502 GO:0039503 GO:0039520 GO:0039564 GO:0039574 GO:0039654 GO:0039663 GO:0039694 GO:0039714 GO:0042025 GO:0044165 GO:0044167 GO:0046718 GO:0046762 GO:0046872 GO:0046983 GO:0055036 GO:0070008 GO:0075509 GO:0075512 GO:0080009
16	0.06	0.188	5.34	0.09	0.27	3icuA	GO:0005737 GO:0005770 GO:0005783 GO:0005794 GO:0005856 GO:0008270 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016874 GO:0031647 GO:0042036 GO:0042787 GO:0046872 GO:0048471 GO:0061462 GO:0061630 GO:1904352
17	0.06	0.162	5.21	0.04	0.23	3b42B	GO:0004871 GO:0006935 GO:0007165 GO:0016020 GO:0016021 GO:0046872
18	0.06	0.143	5.47	0.01	0.20	3oe2A	GO:0000413 GO:0003755 GO:0006457 GO:0016853

Consensus prediction of GO terms

Molecular Function	GO:0051536	GO:0035639	GO:0032550	GO:0032559
GO-Score	0.58	0.50	0.50	0.50
Biological Processes	GO:0016226	GO:0006979
GO-Score	0.68	0.40
Cellular Component	GO:0005615
GO-Score	0.08

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.