I-TASSER results

I-TASSER results for job id Rv1356c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (263 residues)
MLIAGYLTDWRIMTTAQLRPIAPQKLHFSENLSVWVSDAQCRLVVSQPALDPTLWNTYLQ
GALRAYSKHGVECTLDLDAISDGSDTQLFFAAIDIGGDVVGGARVIGPLRSADDSHAVVE
WAGNPGLSAVRKMINDRAPFGVVEVKSGWVNSDAQRSDAIAAALARALPLSMSLLGVQFV
MGTAAAHALDRWRSSGGVIAARIPAAAYPDERYRTKMIWWDRRTLANHAEPKQLSRMLVE
SRKLLRDVEALSATTAATAGAEQ

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MLIAGYLTDWRIMTTAQLRPIAPQKLHFSENLSVWVSDAQCRLVVSQPALDPTLWNTYLQGALRAYSKHGVECTLDLDAISDGSDTQLFFAAIDIGGDVVGGARVIGPLRSADDSHAVVEWAGNPGLSAVRKMINDRAPFGVVEVKSGWVNSDAQRSDAIAAALARALPLSMSLLGVQFVMGTAAAHALDRWRSSGGVIAARIPAAAYPDERYRTKMIWWDRRTLANHAEPKQLSRMLVESRKLLRDVEALSATTAATAGAEQ
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHCCCCCEEEEEHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCHHHCCCCCCCEEEEEEHCCCCCEEEEEEEHCCCCCCCCCCCEEHCCCCCCHHHHHHHHHHHCCCCEEEEEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHCHHHHHHHHHHHCCCEHCCCCCCCCCCCCCCCCEEEEHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
Conf.Score	97555555654456777778886545567888655688856999977898989999999999999999866877465544579997899999879998898899868888866676455479997789999999986888679998775788865568899998877788888879958885368899999864786766888999999865555444334788887679899999999999999998775555555565689
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 \| \| \| \| \| \| \| \| \| \| \| \| \| MLIAGYLTDWRIMTTAQLRPIAPQKLHFSENLSVWVSDAQCRLVVSQPALDPTLWNTYLQGALRAYSKHGVECTLDLDAISDGSDTQLFFAAIDIGGDVVGGARVIGPLRSADDSHAVVEWAGNPGLSAVRKMINDRAPFGVVEVKSGWVNSDAQRSDAIAAALARALPLSMSLLGVQFVMGTAAAHALDRWRSSGGVIAARIPAAAYPDERYRTKMIWWDRRTLANHAEPKQLSRMLVESRKLLRDVEALSATTAATAGAEQ
Prediction	51011213212213235045242541416552313144230200012264436004400410351045310430041540462530100000015633110012032314327422013516544025202510363133000203211235535434300210130122103214031000000310153144331322750332413374140210110232145304343034014404502541541453334444468
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHCCCCCEEEEEHCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCHHHCCCCCCCEEEEEEHCCCCCEEEEEEEHCCCCCCCCCCCEEHCCCCCCHHHHHHHHHHHCCCCEEEEEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHCHHHHHHHHHHHCCCEHCCCCCCCCCCCCCCCCEEEEHCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
MLIAGYLTDWRIMTTAQLRPIAPQKLHFSENLSVWVSDAQCRLVVSQPALDPTLWNTYLQGALRAYSKHGVECTLDLDAISDGSDTQLFFAAIDIGGDVVGGARVIGPLRSADDSHAVVEWAGNPGLSAVRKMINDRAPFGVVEVKSGWVNSDAQRSDAIAAALARALPLSMSLLGVQFVMGTAAAHALDRWRSSGGVIAARIPAAAYPDERYRTKMIWWDRRTLANHAEPKQLSRMLVESRKLLRDVEALSATTAATAGAEQ

2ipiA

0.18

0.16

0.90

0.64

MUSTER

AHTGGGGGNFGIVTRYWFRTPGATGTDPS-QLLPKAPTSTLRHIVTWDALTEEAFTRIIDNHGAWHQSN------SAAGTPYASMHSVFYLNSRAAGQILLDIQIDGGLDGAEAVEPAVQRSTEPWLRATLANKFDTGGFDRTKSKGAYLRKPWT--AAQAATLYRHLSADSQVWGEVSLYSY------------GGKVN-SVPATATAQ-RDSIIKVWM----SATWMDPAHDDANLAWIREIYREIFATTGGVPVPDDRTE

3p2fA

0.15

0.10

0.66

0.72

dPPAS

---------------------------------------MQTFVHEAGRLPAHIAAELGSYRYRVFVEQ-----LKMERDQYDRDDTVYVLGRDANGEICGCARLL----PTTRPYLL--------QEVFPHLLADEAPRSVWELSRFAATAW------SVRPMLAAAVECAARRGARQLIGVTFCSMERMFRRIGVHAHRAGAPVSIDGRMVVACWIDIDAQTLAALDLDPALC----------------------------

3p2fA

0.17

0.11

0.64

0.79

wdPPAS

----------------------------------------MQTFVHEGRLPAHIAAELGSYRYRVFVE---QLKMERDQY-DRDDT-VYVLGRDANGEICGCARLL----PTTRPYLL--------QEVFPHLLADEAPRSVWELSRFAATAW------SVRPMLAAAVECAARRGARQLIGVTFCSMERMFRRIGVHAHRAGAPVSIDGRMV--VACW-DAQTLAALDDPALC-----------------------------

2ipiA

0.19

0.17

0.88

0.63

wMUSTER

AHTGGGGGNFGIVTRYWFRTPGATGTDPS-QLLPKAPTSTLRHIVTWDALTEEAFTRIIDNHGAWHQSNSAAGT-PYASM-----HSVFYLNSRAAGQILLDIQIDGGLDGAEALAAVNEGTGEPWLRATLANKFDTGGFDRTKSKGAYLRKPWT--AAQAATLYRHLSADSQVWGEVSLYSY------------GGKVN-SVPATATADSII-WMSATWM--------DPAHDDANLAWIREIYREIFATTGGVPVPDDRTE

1ro5A

0.17

0.12

0.68

0.72

wPPAS

-------------------------------------GSHMIVQIGR-EFDKKLLGEMHKLRAQVFERKGVIDEMEIDG--DALSP--YYMLIQEDGQVFGCWRIL-D---TTGPYMLKN--------TFPELLHGEAPCSIWELSRFAINSGQKGSSDCTLEAMRALARYSLQNDIQTLVTVTTVGVEKMMIRAGLDV-SRFPHLKIGIER--AVALRINAKTQIALYGG-----VLVEQR---------------------

3p2fA

0.15

0.10

0.65

1.12

dPPAS2

---------------------------------------MQTFVHEAGRLPAHIAAELGSYRYRVFVEQ-----LKMERDQYDRDDTVYVLGRDANGEICGCARLL----PTTRPYLL--------QEVFPHLLADEAPRSVWELSRFAATAW------SVRPMLAAAVECAARRGARQLIGVTFCSMERMFRRIGVHAHRAGAPVSIDGRM--VVACWIDAQTLAALDDPALC-----------------------------

1ro5A

0.15

0.11

0.70

0.74

PPAS

-------------------------------------GSHMIVQIGR-EFDKKLLGEMHKLRAQVFKERGVIDEMEIDG----ALSPYYMLIQE-DGQVFGCWRILDT----TGPYML----KNTFPELLHGKEAPCSPH-IWELSRFAINSGQKGSSDCTLEAMRALARYSLQNDIQTLVTVTTVGVEKMMIRAGLDVSRFGPHLKIGIER--AVALRINAKTQIALYGG-----VLVEQR---------------------

1ro5A

0.15

0.11

0.71

0.97

Env-PPAS

-------------------------------------GSHMIVQIGREEFDKKLLGEMHKLRAQVFKERGVIDEMEIDGY-DALSPYYMLIQED--GQVFGCWRILDT----TGPYMLKNT-----FPELLHGKEAPCSPHIWELSRFAINSGQKGSSDCTLEAMRALARYSLQNDIQTLVTVTTVGVEKMMIRAGLDVSRFGPHLKIGIER--AVALRINAKTQIALYGG-----VLVEQR---------------------

2y08A

0.16

0.14

0.88

0.60

MUSTER

HTGGGVVVRYWLRTAEADVPPEPGRLLPRPPAEVLL----NTTVWPWEGLDEAAFARLVRNHGRWFEQNSGP------DSPWCDLYSVLALTRSQSGALAMTTQLDATGPDAEKVQPHSDTRRLPWLHSTPGIAGDGDMTGRAKIKAAYARRSFD--DRQIGTLYTRLTST-DYDNPAGVVA---------LIAYGGKVN-AVPATAVADSIL-VYVTTWE--------DPAQDPVHVRWIRELYRDVYADTGGVPVPGGAAD

1ro5A

0.12

0.09

0.71

0.60

dPPAS

-------------------------------------GSHMIVQIGREEFDKKLLGEMHKLRAQVFKERSVIDEMEIDGY-DALSPYYMLIQED--GQVFGCWRILDTTGPYMLKNTF---------PELLHGKEAPCSPHIWELSRFAINSGQKGSSDCTLEAMRALARYSLQNDIQTLVTVTTVGVEKMMIRAGLDVSRFGPHLKIGIER--AVALRINAKTQIALYGGVLVEQR--------------------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.32

Estimated TM-score = 0.35±0.12

Estimated RMSD = 13.9±3.9Å

Download Model 2

C-score=-2.98

Download Model 3

C-score=-3.79

Download Model 4

C-score=-4.17

Download Model 5

C-score=-4.40

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1ro5A	0.575	3.23	0.092	0.700
2	3p2fA	0.552	2.92	0.139	0.650
3	4fd7A	0.546	3.40	0.087	0.684
4	2g0bA	0.543	3.30	0.084	0.669
5	4fd5A	0.537	3.35	0.130	0.665
6	4fd4A	0.536	3.40	0.137	0.662
7	1kzfA	0.525	3.77	0.122	0.673
8	3te4A	0.523	3.36	0.080	0.650
9	1yleA	0.516	4.36	0.104	0.696
10	3qb8A	0.515	3.33	0.118	0.643

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.18	13	1qsrA	ACO	Rep, Mult	69,146,147,148,149,150,157,158,159,160,161,162,183,184,186,188,191,192,194
2	0.14	10	2g0bA	NLT	Rep, Mult	66,71,145,146,147,148,165,169,183,184,185,186,192,199,208,211,212,213,219
3	0.03	2	2jddA	3PG	Rep, Mult	69,70,78,146,147,186,211,214
4	0.01	1	2hbkA	MN	Rep, Mult	76,153
5	0.01	1	5icvB	III	Rep, Mult	69,71,72,73,144,146,208,209
6	0.01	1	1hizA	GLA	Rep, Mult	10,144,192
7	0.01	1	1kfvA	NUC	Rep, Mult	104,116,130,131,132,140
8	0.01	1	1aniA	PO4	Rep, Mult	191,192,214
9	0.01	1	2ch1A	LLP	Rep, Mult	213,215
10	0.01	1	3p2hA	MTA	Rep, Mult	78,79,118,131,147
11	0.01	1	2i14A	ZN	Rep, Mult	187,221,247
12	0.01	1	3gwzD	CA	Rep, Mult	210,212
13	0.01	1	3mflB	DHY	Rep, Mult	52,66

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.163	1ro5A	0.575	3.23	0.092	0.700	2.3.1.184	NA
2	0.067	2cntD	0.463	2.77	0.113	0.536	2.3.1.128	150,188
3	0.066	2vqyA	0.483	3.53	0.112	0.608	2.3.1.82	43,167,188
4	0.066	1yghA	0.466	3.34	0.163	0.570	2.3.1.48	NA
5	0.066	1cm0A	0.454	3.06	0.151	0.547	2.3.1.48	90,106
6	0.060	1qsmD	0.422	3.50	0.086	0.525	2.3.1.48	66
7	0.060	1mj9A	0.430	3.80	0.080	0.544	2.3.1.48	74,155,189
8	0.060	3iu2A	0.459	3.96	0.103	0.608	2.3.1.97	NA
9	0.060	3b9jI	0.213	5.63	0.023	0.342	1.17.1.4,1.17.3.2	64,70,77,83
10	0.060	1k4jA	0.525	3.80	0.115	0.673	2.3.1.184	82,93,150
11	0.060	1p4nA	0.428	3.87	0.079	0.547	2.3.2.10	200,202
12	0.060	1yvoB	0.451	3.40	0.159	0.555	2.3.-.-	143
13	0.060	3b9jC	0.415	6.30	0.038	0.711	1.17.3.2,1.17.1.4	NA
14	0.060	2e1qA	0.340	6.75	0.047	0.627	1.17.3.2,1.17.1.4	NA
15	0.060	1vhsA	0.450	3.57	0.141	0.563	2.3.1.183	NA
16	0.060	2rc4A	0.429	3.75	0.060	0.544	2.3.1.48,	NA
17	0.060	1yleA	0.516	4.36	0.104	0.696	2.3.1.109	44,46,77
18	0.060	1dgjA	0.433	6.29	0.063	0.753	1.2.-.-	NA
19	0.060	1t3tA	0.428	6.01	0.071	0.715	6.3.5.3	61
20	0.060	3d2pB	0.424	3.46	0.051	0.513	2.3.1.1	170,175
21	0.060	1q2yA	0.451	2.60	0.117	0.521	2.3.1.-	NA
22	0.060	1nmtA	0.438	3.80	0.046	0.570	2.3.1.97	66,144,161,189
23	0.060	2p0wA	0.430	3.91	0.034	0.540	2.3.1.48	NA
24	0.060	1z4rA	0.454	3.09	0.154	0.547	2.3.1.48	90,106

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.16	0.475	2.57	0.12	0.55	1xebA	GO:0004596 GO:0006474 GO:0008080 GO:0031248
1	0.12	0.457	3.36	0.08	0.57	3ld2B	GO:0008080 GO:0016740
2	0.12	0.546	3.40	0.09	0.68	4fd7A	GO:0008080
3	0.11	0.536	3.40	0.14	0.66	4fd4A	GO:0008080
4	0.10	0.483	2.95	0.09	0.58	1tiqA	GO:0004145 GO:0004596 GO:0006474 GO:0008080 GO:0016740 GO:0016746 GO:0030435 GO:0031248 GO:0043939
5	0.10	0.448	2.86	0.11	0.52	1y9kB	GO:0004596 GO:0006474 GO:0008080 GO:0016740 GO:0016746 GO:0031248
6	0.09	0.563	3.00	0.12	0.67	3p2hA	GO:0009372 GO:0016740 GO:0061579
7	0.09	0.450	3.47	0.10	0.56	4rs2B	GO:0008080 GO:0016740 GO:0016746
8	0.08	0.488	3.31	0.04	0.60	2oh1A	GO:0008080 GO:0016740
9	0.07	0.451	2.60	0.12	0.52	1q2yA	GO:0008080 GO:0016740 GO:0016746
10	0.07	0.457	2.98	0.07	0.55	3efaA	GO:0008080 GO:0016740 GO:0016746
11	0.07	0.482	3.09	0.12	0.59	2qecA	GO:0008080 GO:0016740
12	0.07	0.454	2.76	0.06	0.53	2bswA	GO:0008080 GO:0016740
13	0.07	0.575	3.23	0.09	0.70	1ro5A	GO:0007165 GO:0009372 GO:0016740 GO:0061579
14	0.07	0.457	2.97	0.10	0.54	2r7hB	GO:0008080 GO:0016740
15	0.07	0.525	3.77	0.12	0.67	1kzfA	GO:0009372 GO:0009405 GO:0016740 GO:0061579
16	0.07	0.540	3.48	0.13	0.67	4fd6A	GO:0008080
17	0.07	0.523	3.36	0.08	0.65	3te4A	GO:0004059 GO:0004060 GO:0005737 GO:0006584 GO:0008080 GO:0016740 GO:0016746 GO:0030187 GO:0030431 GO:0042420 GO:0042429 GO:0045187 GO:0046334 GO:0048066
18	0.07	0.448	3.90	0.13	0.59	1u6mA	GO:0008080 GO:0016740
19	0.07	0.461	3.37	0.11	0.57	2jlmD	GO:0008080 GO:0016740
20	0.06	0.454	3.31	0.13	0.56	4mbuA	GO:0008080 GO:0016740
21	0.06	0.423	3.84	0.13	0.53	5f47B	GO:0008080
22	0.06	0.403	3.62	0.07	0.51	2beiB	GO:0004145 GO:0005737 GO:0008080 GO:0009447 GO:0016740 GO:0016746 GO:0032918 GO:0032919 GO:0032920 GO:0046204 GO:0070062
23	0.06	0.295	5.55	0.04	0.47	4my0C	GO:0008080 GO:0016740
24	0.06	0.405	3.88	0.12	0.51	5hmnB	GO:0008080
25	0.06	0.398	3.88	0.07	0.51	3t90A	GO:0004343 GO:0005783 GO:0005789 GO:0006044 GO:0006048 GO:0008080 GO:0016020 GO:0016740 GO:0016746
26	0.06	0.397	2.73	0.13	0.46	4qusB	GO:0004596 GO:0006474 GO:0008080 GO:0016740 GO:0016746 GO:0016747 GO:0031248

Consensus prediction of GO terms

Molecular Function	GO:0034212
GO-Score	0.48
Biological Processes	GO:0031365	GO:0006473
GO-Score	0.48	0.48
Cellular Component	GO:1902493	GO:0044424
GO-Score	0.48	0.48

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.