I-TASSER results

I-TASSER results for job id Rv1321

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (226 residues)
MSRVRLVIAQCTVDYIGRLTAHLPSARRLLLFKADGSVSVHADDRAYKPLNWMSPPCWLT
EESGGQAPVWVVENKAGEQLRITIEGIEHDSSHELGVDPGLVKDGVEAHLQALLAEHIQL
LGEGYTLVRREYMTAIGPVDLLCSDERGGSVAVEIKRRGEIDGVEQLTRYLELLNRDSVL
APVKGVFAAQQIKPQARILATDRGIRCLTLDYDTMRGMDSGEYRLF

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MSRVRLVIAQCTVDYIGRLTAHLPSARRLLLFKADGSVSVHADDRAYKPLNWMSPPCWLTEESGGQAPVWVVENKAGEQLRITIEGIEHDSSHELGVDPGLVKDGVEAHLQALLAEHIQLLGEGYTLVRREYMTAIGPVDLLCSDERGGSVAVEIKRRGEIDGVEQLTRYLELLNRDSVLAPVKGVFAAQQIKPQARILATDRGIRCLTLDYDTMRGMDSGEYRLF
Prediction	CCCEEEEEEEEEEEEEEHCCCCCCCCCEEEEEHCCCCEEEEHCCCCCCCCCCCCCCCEEEEEHCCCEEEEEEHCCCCCEEEEEEEEEEEEEEEHCCCCCCEEEHCCHHHHHHHHHHHHHHHCCCEEEEEEEEHCCCCCEEEEEHCCCCCEEEEEEHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHCCCCCHHHHHHHHHHCCEEEEHCHHHCCCCCCCCCCCC
Conf.Score	9968999999999998754467888878999966995899758988777888899868999978977999997588969999997677777654578988467556999999999958885899699999997589867799988999999999976899569999999999999777778857999857799999999999899699976777578888777889
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 \| \| \| \| \| \| \| \| \| \| \| MSRVRLVIAQCTVDYIGRLTAHLPSARRLLLFKADGSVSVHADDRAYKPLNWMSPPCWLTEESGGQAPVWVVENKAGEQLRITIEGIEHDSSHELGVDPGLVKDGVEAHLQALLAEHIQLLGEGYTLVRREYMTAIGPVDLLCSDERGGSVAVEIKRRGEIDGVEQLTRYLELLNRDSVLAPVKGVFAAQQIKPQARILATDRGIRCLTLDYDTMRGMDSGEYRLF
Prediction	5620000002030404222434155041000011430010024755250331134423133345741202213545623020204402201234045445143412153015102620530266041034225173020000021674310000002445360020023004104556345401000002502650251057360401303275145365764627
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCEEEEEEEEEEEEEEHCCCCCCCCCEEEEEHCCCCEEEEHCCCCCCCCCCCCCCCEEEEEHCCCEEEEEEHCCCCCEEEEEEEEEEEEEEEHCCCCCCEEEHCCHHHHHHHHHHHHHHHCCCEEEEEEEEHCCCCCEEEEEHCCCCCEEEEEEHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHCCCCCHHHHHHHHHHCCEEEEHCHHHCCCCCCCCCCCC
MSRVRLVIAQCTVDYIGRLTAHLPSARRLLLFKADGSVSVHADDRAYKPLNWMSPPCWLTEESGGQAPVWVVENKAGEQLRITIEGIEHDSSHELGVDPGLVKDGVEAHLQALLAEHIQLLGEGYTLVRREYMTAIGPVDLLCSDERGGSVAVEIKRRGEIDGVEQLTRYLELLNRDSVLAPVKGVFAAQQIKPQARILATDRGIRCLTLDYDTMRGMDSGEYRLF

2vldA

0.24

0.22

0.88

2.55

MUSTER

HGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQN-KKREPVNWQPPGSKVTFKE----NSISIRRRPYERLEVEIIEPYSLVVFLAEDYEE---SEAE---ANLIFENPRVIEEGFKPIYREKPIRHGIVD-VGVDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEEYG-ENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA

0.23

0.21

0.88

4.89

dPPAS

HGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQN-KKREPVNWQPPGSKV----TFKENSISIRRRPYERLEVEIIEPYSLVVFLAEDYEE------SEAEANLIFENPRVIEEGFKPIYREKPIRHGIVD-VGVDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEEYG-ENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA

0.24

0.21

0.88

5.36

wdPPAS

HGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQN-KKREPVNWQPPGSKVTFKE----NSISIRRRPYERLEVEIIEPYSLVVFLAEDYE------ESEAEANLIFENPRVIEEGFKPIYREKPIRHGIVDVG-VDKDGNIVVLELKRRKADLHAVSQKRYVDSLKE-EYGENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA

0.24

0.21

0.88

3.02

wMUSTER

HGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQN-KKREPVNWQPPGSKVTFKE----NSISIRRRPYERLEVEIIEPYSLVVFLAEDYEE------SEAEANLIFENPRVIEEGFKPIYREKPIRHGIVD-VGVDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEEYG-ENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA

0.24

0.21

0.88

4.63

wPPAS

HGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQN-KKREPVNWQPPGSKVTFKE----NSISIRRRPYERLEVEIIEPYSLVVFLAEDYEE------SEAEANLIFENPRVIEEGFKPIYREKPIRHGIVDVG-VDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEEY-GENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA

0.24

0.22

0.88

13.10

dPPAS2

HGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQN-KKREPVNWQPPGSKVTFKE----NSISIRRRPYERLEVEIIEPYSLVVFLAEDYEE---SEAE---ANLIFENPRVIEEGFKPIYREKPIRHGIVD-VGVDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEEYG-ENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA

0.25

0.22

0.88

3.89

PPAS

HGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQN-KKREPVNWQPPGSKVTFKE----NSISIRRRPYERLEVEIIEPYSLVVFLAED-------YEESEAANLIFENPRVIEEGFKPIYREKPIRHGIVD-VGVDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEEYG-ENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA

0.25

0.22

0.88

4.79

Env-PPAS

HGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQN-KKREPVNWQPPGSKVTFKE----NSISIRRRPYERLEVEIIEPYSLVVFLAED-------YEESEAANLIFENPRVIEEGFKPIYREKPIRHGIVD-VGVDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEE-YGENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA2

0.26

0.12

0.46

1.21

MUSTER

-------------------------------------------------------------------------------------------------------SEAE---ANLIFENPRVIEEGFKPIYREKPIRHGIVD-VGVDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEEYG-ENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

2vldA2

0.26

0.12

0.46

2.59

dPPAS

-------------------------------------------------------------------------------------------------------SEAE---ANLIFENPRVIEEGFKPIYREKPIRHGIVD-VGVDKDGNIVVLELKRRKADLHAVSQKRYVDSLKEEYG-ENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPP-------------

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.22

Estimated TM-score = 0.74±0.11

Estimated RMSD = 5.1±3.3Å

Download Model 2

C-score=0.14

Download Model 3

C-score=-0.14

Download Model 4

C-score=-2.65

Download Model 5

C-score=-2.72

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2vldA	0.842	1.31	0.250	0.885
2	5gkeA	0.520	4.08	0.188	0.668
3	2vdcA	0.456	5.61	0.037	0.748
4	3jb9A	0.456	5.39	0.038	0.739
5	1llwA2	0.455	5.57	0.032	0.748
6	3dy5A	0.443	5.57	0.065	0.730
7	1dc1B	0.441	4.70	0.079	0.620
8	1ii0B	0.440	4.65	0.062	0.633
9	1gph1	0.438	5.34	0.063	0.695
10	2xt6A	0.435	5.83	0.076	0.748

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.13	7	4e5iA	MN	Rep, Mult	107,140,154,155,156
2	0.10	5	3odhF	CA	Rep, Mult	140,154
3	0.06	3	4r5qA	MG	Rep, Mult	140,154,155,164
4	0.04	2	1a9x0	III	Rep, Mult	193,197,198,206,207,208
5	0.04	2	3odhA	CA	Rep, Mult	131,140
6	0.02	1	1c3oA	GLN	Rep, Mult	112,113,142,144
7	0.02	1	2iubF	MG	Rep, Mult	56,131
8	0.02	1	5gkhA	MG	Rep, Mult	138,154,167,170
9	0.02	1	1t36A	U	Rep, Mult	154,155,169,187,188,199,200,205
10	0.02	1	3zszA	OM2	Rep, Mult	166,170
11	0.02	1	2axtH	CLA	Rep, Mult	171,174
12	0.02	1	3fg4C	ACD	Rep, Mult	132,141,170,171,174

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1ikpA	0.407	5.91	0.059	0.717	2.4.2.-	NA
2	0.060	3dy5A	0.443	5.57	0.065	0.730	1.13.11.40,4.2.1.92	NA
3	0.060	3b8eA	0.394	5.60	0.039	0.664	3.6.3.9	NA
4	0.060	3dpgA	0.400	4.06	0.065	0.522	3.1.21.4	156
5	0.060	1ecfB	0.424	5.44	0.048	0.690	2.4.2.14	NA
6	0.060	3mosA	0.431	5.32	0.065	0.695	2.2.1.1	NA
7	0.060	1z0hB	0.337	6.08	0.018	0.602	3.4.24.69	191
8	0.060	1ea0A	0.456	5.61	0.037	0.748	1.4.1.13	NA
9	0.060	3b8eC	0.404	5.78	0.070	0.686	3.6.3.9	108,163
10	0.060	1trkA	0.422	5.79	0.059	0.735	2.2.1.1	109,114
11	0.060	2vdcF	0.462	5.45	0.031	0.743	1.4.1.13	NA
12	0.060	1mpxA	0.399	6.37	0.038	0.757	3.2.1.43	NA
13	0.060	2jgdA	0.429	5.80	0.061	0.730	1.2.4.2	160
14	0.060	1itzB	0.422	5.94	0.043	0.761	2.2.1.1	171
15	0.060	1rzuB	0.394	5.81	0.076	0.664	2.4.1.21	163,200
16	0.060	1gph1	0.438	5.34	0.063	0.695	2.4.2.14	NA
17	0.060	1itzA	0.422	5.85	0.038	0.752	2.2.1.1	NA
18	0.060	2np0A	0.334	5.91	0.026	0.562	3.4.24.69	NA
19	0.060	2e6kA	0.409	5.90	0.048	0.735	2.2.1.1	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.49	0.855	1.31	0.25	0.90	2vldA	GO:0000014 GO:0003677 GO:0004518 GO:0004519 GO:0005737 GO:0006259 GO:0016787 GO:0042802 GO:0090305
1	0.12	0.456	5.61	0.04	0.75	2vdcA	GO:0003824 GO:0004355 GO:0006537 GO:0006541 GO:0006807 GO:0008152 GO:0008652 GO:0015930 GO:0016491 GO:0016638 GO:0046872 GO:0051536 GO:0051538 GO:0055114 GO:0097054
2	0.09	0.380	6.18	0.04	0.67	1llwA	GO:0003824 GO:0005737 GO:0006537 GO:0006541 GO:0006807 GO:0008152 GO:0008652 GO:0015930 GO:0016041 GO:0016491 GO:0016638 GO:0019676 GO:0046872 GO:0051536 GO:0051538 GO:0055114 GO:0097054
3	0.07	0.462	5.59	0.06	0.75	1ofdA	GO:0003824 GO:0005737 GO:0006537 GO:0006541 GO:0006807 GO:0008152 GO:0008652 GO:0015930 GO:0016041 GO:0016491 GO:0016638 GO:0019676 GO:0046872 GO:0051536 GO:0051538 GO:0055114 GO:0097054
4	0.07	0.438	5.34	0.06	0.69	1gph1	GO:0000287 GO:0003824 GO:0004044 GO:0006164 GO:0006189 GO:0006541 GO:0008152 GO:0009113 GO:0009116 GO:0016740 GO:0016757 GO:0046872 GO:0051536 GO:0051539
5	0.07	0.456	5.61	0.04	0.75	1ea0A	GO:0003824 GO:0004355 GO:0006537 GO:0006541 GO:0006807 GO:0008152 GO:0008652 GO:0015930 GO:0016491 GO:0016638 GO:0046872 GO:0051536 GO:0051538 GO:0055114 GO:0097054
6	0.06	0.364	6.34	0.05	0.68	5gapA	GO:0000244 GO:0000350 GO:0000386 GO:0000389 GO:0000398 GO:0003676 GO:0003723 GO:0005634 GO:0005681 GO:0005682 GO:0006397 GO:0008380 GO:0017070 GO:0030529 GO:0030619 GO:0030620 GO:0030623 GO:0046540 GO:0071013 GO:0097157
7	0.06	0.268	5.65	0.07	0.45	1qb7A	GO:0000166 GO:0003999 GO:0009116 GO:0016740 GO:0016757
8	0.06	0.275	6.43	0.04	0.53	4bb9A	GO:0000060 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005975 GO:0009750 GO:0010827 GO:0030246 GO:0033132 GO:0046415 GO:0070095 GO:0070328
9	0.06	0.220	6.35	0.02	0.41	3bdzA	GO:0004497 GO:0005506 GO:0016491 GO:0016705 GO:0016709 GO:0020037 GO:0046232 GO:0046872 GO:0055114
10	0.06	0.296	5.42	0.02	0.47	1kbiA	GO:0003824 GO:0004460 GO:0005739 GO:0005743 GO:0005758 GO:0005829 GO:0006089 GO:0006626 GO:0010181 GO:0016491 GO:0020037 GO:0046872 GO:0055114 GO:0070469
11	0.06	0.298	5.28	0.04	0.46	3r2gA	GO:0003824 GO:0003938 GO:0016491 GO:0055114
12	0.06	0.309	5.84	0.05	0.54	2w91A	GO:0005576 GO:0005618 GO:0005737 GO:0016020 GO:0033925
13	0.06	0.292	4.65	0.05	0.42	1n7kA	GO:0003824 GO:0004139 GO:0005737 GO:0005975 GO:0009264 GO:0016052 GO:0016829 GO:0046386 GO:0051262
14	0.06	0.284	5.19	0.03	0.42	3ck5A	GO:0003824 GO:0008152 GO:0009063 GO:0046872
15	0.06	0.346	5.75	0.02	0.59	3zefE	GO:0000244 GO:0000350 GO:0000386 GO:0000389 GO:0000398 GO:0003676 GO:0003723 GO:0005634 GO:0005681 GO:0005682 GO:0006397 GO:0008380 GO:0017070 GO:0030529 GO:0030619 GO:0030620 GO:0030623 GO:0046540 GO:0071013 GO:0097157
16	0.06	0.297	5.49	0.02	0.48	1ldcB	GO:0003824 GO:0004460 GO:0005739 GO:0005743 GO:0005758 GO:0005829 GO:0006089 GO:0006626 GO:0010181 GO:0016491 GO:0020037 GO:0046872 GO:0055114 GO:0070469
17	0.06	0.303	6.36	0.08	0.58	3t0pB	GO:0003677 GO:0003887 GO:0005737 GO:0006260 GO:0008408 GO:0009360 GO:0016740 GO:0016779 GO:0071897 GO:0090305
18	0.06	0.294	5.19	0.03	0.46	3sgzA	GO:0003824 GO:0003973 GO:0005777 GO:0010181 GO:0016491 GO:0018924 GO:0051260 GO:0052852 GO:0052853 GO:0052854 GO:0055114

Consensus prediction of GO terms

Molecular Function	GO:0051540	GO:0043169	GO:0016638	GO:0000014	GO:0042802	GO:0003677
GO-Score	0.60	0.60	0.50	0.49	0.49	0.49
Biological Processes	GO:0006520	GO:0006537	GO:0090305	GO:0006259
GO-Score	0.60	0.50	0.49	0.49
Cellular Component	GO:0005737
GO-Score	0.56

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.