I-TASSER results

I-TASSER results for job id Rv1301

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (217 residues)
MTETFDCADPEQRSRGIVSAVGAIKAGQLVVMPTDTVYGIGADAFDSSAVAALLSAKGRG
RDMPVGVLVGSWHTIEGLVYSMPDGARELIRAFWPGALSLVVVQAPSLQWDLGDAHGTVM
LRMPLHPVAIELLREVGPMAVSSANISGHPPPVDAEQARSQLGDHVAVYLDAGPSEQQAG
STIVDLTGATPRVLRPGPVSTERIAEVLGVDAASLFG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 \| \| \| \| \| \| \| \| \| \| MTETFDCADPEQRSRGIVSAVGAIKAGQLVVMPTDTVYGIGADAFDSSAVAALLSAKGRGRDMPVGVLVGSWHTIEGLVYSMPDGARELIRAFWPGALSLVVVQAPSLQWDLGDAHGTVMLRMPLHPVAIELLREVGPMAVSSANISGHPPPVDAEQARSQLGDHVAVYLDAGPSEQQAGSTIVDLTGATPRVLRPGPVSTERIAEVLGVDAASLFG
Prediction	CCEEEHCCCCCCCHHHHHHHHHHHHHCCEEEHCCCCCEEHCCCCCCHHHHHHHHHHHCCCCCCCCEEEHCCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEHCCCCCCCCCCCCCCEEEEHCCCCHHHHHHHHHHCCCHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEHCCCCCCCCCCEEEHCCCCCEEEHCCCCCCHHHHHHHHCCCHHHCCC
Conf.Score	9889987998888999999999999899699858887665468999999999999989999999589979999999999769999999999979998899996899888554899996999958999999999999994646788999999999999999989988699958788888875789856996899858999999999998998666579
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 \| \| \| \| \| \| \| \| \| \| MTETFDCADPEQRSRGIVSAVGAIKAGQLVVMPTDTVYGIGADAFDSSAVAALLSAKGRGRDMPVGVLVGSWHTIEGLVYSMPDGARELIRAFWPGALSLVVVQAPSLQWDLGDAHGTVMLRMPLHPVAIELLREVGPMAVSSANISGHPPPVDAEQARSQLGDHVAVYLDAGPSEQQAGSTIVDLTGATPRVLRPGPVSTERIAEVLGVDAASLFG
Prediction	7243150447644562053005005744000000200400001032660042015037146722000001116303510560264024005533030000002337601530257340000001315101420651410000101366544113063026206730100000351744331110213774020014360436202600624155268
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCEEEHCCCCCCCHHHHHHHHHHHHHCCEEEHCCCCCEEHCCCCCCHHHHHHHHHHHCCCCCCCCEEEHCCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEHCCCCCCCCCCCCCCEEEEHCCCCHHHHHHHHHHCCCHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEHCCCCCCCCCCEEEHCCCCCEEEHCCCCCCHHHHHHHHCCCHHHCCC
MTETFDCADPEQRSRGIVSAVGAIKAGQLVVMPTDTVYGIGADAFDSSAVAALLSAKGRGRDMPVGVLVGSWHTIEGLVYSMPDGARELIRAFWPGALSLVVVQAPSLQWDLGDAHGTVMLRMPLHPVAIELLREVGPMAVSSANISGHPPPVDAEQARSQLGDHVAVYLDAGPSEQQAGSTIVDLTGATPRVLRPGPVSTERIAEVLGVDAASLFG

2eqaA1

0.33

0.32

0.97

4.55

MUSTER

-TQIIKIDPLNPEIDKIKIAADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPGPLTFVLKKTERVPKEVTAGLDTVAVR-PAHPIALQLIRESGPIAAPSANLATRPSPTKAEDVIVDLNGRVDVIIDGGHTFFGVESTIINVTVE-PVLLRPGPFTIEELKKLFGEIVI----

2eqaA1

0.33

0.32

0.97

11.72

dPPAS

-TQIIKIDPLNPEIDKIKIAADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPGPLTFVLKKTERVPKEVTAGLDTVAVR-PAHPIALQLIRESGPIAAPSANLATRPSPTKAEDVIVDLNGRVDVIIDGGHTFFGVESTIINVTVE-PVLLRPGPFTIEELKKLFGEIVI----

2eqaA1

0.33

0.32

0.97

9.74

wdPPAS

-TQIIKIDPLNPEIDKIKIAADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPGPLTFVLKKTERVPKEVTAGLDTVAVR-PAHPIALQLIRESGPIAAPSANLATRPSPTKAEDVIVDLNGRVDVIIDGGHTFFGVESTIINVTVE-PVLLRPGPFTIEELKKLFGEIVI----

2eqaA1

0.33

0.31

0.96

5.12

wMUSTER

--QIIKIDPLNPEIDKIKIAADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPGPLTFVLKKTERVPKEVTAGLDTVAVR-PAHPIALQLIRESGPIAAPSANLATRPSPTKAEDVIVDLNGRVDVIIDGGHTFFGVESTIINVTVE-PVLLRPGPFTIEELKKLFGEIVI----

2eqaA1

0.33

0.32

0.97

5.21

wPPAS

-TQIIKIDPLNPEIDKIKIAADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPGPLTFVLKKTERVPKEVTAGLDTVAVR-PAHPIALQLIRESGPIAAPSANLATRPSPTKAEDVIVDLNGRVDVIIDGGHTFFGVESTIINVTVE-PVLLRPGPFTIEELKKLFGEIVI----

2eqaA1

0.33

0.32

0.97

11.23

dPPAS2

-TQIIKIDPLNPEIDKIKIAADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPGPLTFVLKKTERVPKEVTAGLDTVAVR-PAHPIALQLIRESGPIAAPSANLATRPSPTKAEDVIVDLNGRVDVIIDGGHTFFGVESTIINVTVE-PVLLRPGPFTIEELKKLFGEIVI----

2eqaA1

0.33

0.32

0.97

4.94

PPAS

-TQIIKIDPLNPEIDKIKIAADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPGPLTFVLKKTERVPKEVTAGLDTVAVR-PAHPIALQLIRESGPIAAPSANLATRPSPTKAEDVIVDLNGRVDVIIDGGHTFFGVESTIINVTVE-PVLLRPGPFTIEELKKLFGEIVI----

2eqaA1

0.33

0.32

0.97

6.88

Env-PPAS

-TQIIKIDPLNPEIDKIKIAADVIRNGGTVAFPTETVYGLGANAFDGNACLKIFQAKNRPVDNPLIVHIADFNQLFEVAKDIPDKVLEIAQIVWPGPLTFVLKKTERVPKEVTAGLDTVAVR-PAHPIALQLIRESGPIAAPSANLATRPSPTKAEDVIVDLNGRVDVIIDGGHTFFGVESTIINVTVE-PVLLRPGPFTIEELKKLFGEIVI----

1k7jA

0.21

0.19

0.92

3.95

MUSTER

-SQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKIEDKNA-ERICRIRQLPDGHNFTL-CRDLSELSTYSF-VDNVAFRLK-NNTPGNYTFILKGTKEVPRRLLQEKRKTIGRVPSNPIAQALLEALGEPLSTSLLPGSEFTESDPEEIKDRLEKQVDLIIHGGYLG-QKPTTVIDLTDDTPVVVREGVGDVKPFL------------

1jcuA

0.28

0.27

0.96

10.25

dPPAS

-MLIRKITRKNPSPDVLEEAISVMEGGGIVIYPTDTIYGLGVNALDEDAVRRLFRVKGRSPHKPVSICVSCVDEIPRFS-RPSGDAMELMERILPGPYTVVLERNELIPDVITGGSSRVGIRVPDDEICRRIAARF-PVTATSANISGKPPSPRLEEIVRDLDA-VDLVLDAGDCLDMEPSTVIDLTVNPPRVLRRGKGPLDPVLLRGAGDV-----

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 4 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.92

Estimated TM-score = 0.99±0.04

Estimated RMSD = 1.8±1.5Å

Download Model 2

C-score=-1.31

Download Model 3

C-score=-4.61

Download Model 4

C-score=-4.26

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2eqaA1	0.954	0.67	0.324	0.968
2	1k7jA	0.818	1.97	0.230	0.903
3	3l7vA	0.797	2.34	0.205	0.908
4	3vthA	0.785	3.13	0.178	0.940
5	3tspA	0.762	2.90	0.236	0.899
6	4g9iA	0.760	3.22	0.181	0.931
7	1hruA	0.735	2.40	0.242	0.839
8	1jcuA	0.669	3.37	0.237	0.843
9	3venA2	0.597	2.87	0.110	0.710
10	4p6cA	0.517	4.20	0.099	0.710

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.81	16	2eqaA	AMP	Rep, Mult	59,63,64,65,66,118,120,142,143,183,194,195
2	0.29	4	3ajeA	THR	Rep, Mult	36,37,38,39,66,68,122,141,180,181
3	0.03	1	3vthB	MG	Rep, Mult	143,180,195
4	0.02	1	3lrjA	SO4	Rep, Mult	38,122,124,126,130
5	0.02	1	1hbmA	NA	Rep, Mult	112,115,117,118,119
6	0.02	1	3vesA	MN	Rep, Mult	57,143

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1k4iA	0.510	4.14	0.109	0.691	4.1.99.12,5.4.99.-	NA
2	0.060	1pvwA	0.490	4.43	0.075	0.682	4.1.99.12,5.4.99.-	NA
3	0.060	1e6vA	0.404	5.55	0.093	0.691	2.8.4.1	NA
4	0.060	1tksA	0.502	4.08	0.103	0.677	4.1.99.12,5.4.99.-	NA
5	0.060	1e6yA	0.405	5.26	0.103	0.664	2.8.4.1	NA
6	0.060	2uveA	0.392	6.45	0.054	0.751	3.2.1.82	114
7	0.060	1larA	0.404	5.58	0.061	0.677	3.1.3.48	NA
8	0.060	1adyD	0.326	5.61	0.053	0.553	6.1.1.21	162
9	0.060	2c6fA	0.390	5.93	0.052	0.687	3.4.15.1	NA
10	0.060	1j36A	0.395	5.91	0.026	0.691	3.4.15.1	42
11	0.060	1i1iP	0.350	5.88	0.037	0.599	3.4.24.16	NA
12	0.060	1snnA	0.494	4.41	0.075	0.687	4.1.99.12	99
13	0.060	1k4lA	0.510	4.10	0.115	0.691	4.1.99.12	27
14	0.060	2z8gB	0.390	5.93	0.039	0.691	3.2.1.57	NA
15	0.060	1y4wA	0.396	5.80	0.072	0.682	3.2.1.80	NA
16	0.060	2b3oA	0.390	5.66	0.044	0.654	3.1.3.48	NA
17	0.060	2fy3A	0.396	5.41	0.037	0.659	2.3.1.6	NA
18	0.060	2gfiA	0.390	5.67	0.066	0.668	3.1.3.8	NA
19	0.060	2pm8A	0.386	5.66	0.056	0.659	3.1.1.8	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.42	0.959	0.68	0.32	0.97	4e1bA	GO:0000166 GO:0003725 GO:0005524 GO:0005737 GO:0008033 GO:0016740 GO:0016779 GO:0061710
1	0.41	0.845	1.95	0.23	0.93	1k7jA	GO:0003725 GO:0005829
2	0.33	0.828	2.13	0.20	0.93	3l7vA	GO:0003725
3	0.30	0.669	3.37	0.24	0.84	1jcuA	GO:0003725
4	0.30	0.651	3.47	0.23	0.83	2mx1A	GO:0000049 GO:0000166 GO:0002949 GO:0003723 GO:0003725 GO:0005524 GO:0005737 GO:0005829 GO:0006364 GO:0006450 GO:0008033 GO:0016740 GO:0016779 GO:0042254 GO:0061710
5	0.26	0.761	2.92	0.24	0.90	3tsqA	GO:0003725 GO:0008270 GO:0016743 GO:0046872 GO:0046944
6	0.06	0.345	5.76	0.06	0.58	4u3wA	GO:0008152 GO:0016491 GO:0016620 GO:0046872 GO:0055114
7	0.06	0.309	5.97	0.04	0.54	4eziA	GO:0004806 GO:0016042
8	0.06	0.284	6.29	0.03	0.52	3vueA	GO:0004373 GO:0009501 GO:0009507 GO:0009536 GO:0016740 GO:0016757 GO:0019252 GO:0033840
9	0.06	0.278	5.49	0.03	0.47	1sg9A	GO:0003676 GO:0006479 GO:0008168 GO:0008276 GO:0016740 GO:0018364 GO:0032259 GO:0036009
10	0.06	0.303	6.56	0.04	0.59	3eojA	GO:0015979 GO:0016168 GO:0018298 GO:0042314 GO:0046872 GO:0055114
11	0.06	0.325	6.08	0.06	0.59	1ixkA	GO:0003723 GO:0006364 GO:0008168 GO:0008757 GO:0032259
12	0.06	0.324	5.84	0.09	0.59	2frxA	GO:0003723 GO:0005737 GO:0006364 GO:0008168 GO:0008649 GO:0008757 GO:0009383 GO:0016434 GO:0016740 GO:0031167 GO:0032259 GO:0070475
13	0.06	0.280	6.18	0.03	0.51	4mecB	GO:0001525 GO:0001666 GO:0002246 GO:0004392 GO:0004630 GO:0004871 GO:0005634 GO:0005730 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005901 GO:0006644 GO:0006788 GO:0006879 GO:0006915 GO:0006979 GO:0007264 GO:0008217 GO:0008219 GO:0008285 GO:0008630 GO:0010656 GO:0016020 GO:0016491 GO:0019899 GO:0020037 GO:0031670 GO:0032764 GO:0034101 GO:0034395 GO:0034605 GO:0035094 GO:0035556 GO:0042167 GO:0042168 GO:0042542 GO:0042803 GO:0043123 GO:0043231 GO:0043305 GO:0043392 GO:0043433 GO:0043524 GO:0043619 GO:0043627 GO:0045766 GO:0046872 GO:0048471 GO:0048661 GO:0048662 GO:0051090 GO:0051260 GO:0055072 GO:0055114 GO:0071243 GO:0071276 GO:0071456 GO:0097421 GO:1902042 GO:1904036 GO:1904706
14	0.06	0.303	5.92	0.05	0.55	3n75A	GO:0003824 GO:0005737 GO:0006520 GO:0006554 GO:0008923 GO:0016829 GO:0016831 GO:0042802
15	0.06	0.306	5.80	0.08	0.53	3jcty	GO:0000054 GO:0000463 GO:0000466 GO:0003743 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006364 GO:0006412 GO:0006413 GO:0030687 GO:0042254 GO:0042256 GO:0042273 GO:0042802 GO:0043022 GO:0043023 GO:1902626
16	0.06	0.276	6.37	0.03	0.52	1ix3A	GO:0001525 GO:0001666 GO:0002246 GO:0004392 GO:0004630 GO:0004871 GO:0005634 GO:0005730 GO:0005783 GO:0005789 GO:0005829 GO:0005886 GO:0005901 GO:0006644 GO:0006788 GO:0006879 GO:0006915 GO:0006979 GO:0007264 GO:0008217 GO:0008219 GO:0008285 GO:0008630 GO:0010656 GO:0016020 GO:0016491 GO:0019899 GO:0020037 GO:0031670 GO:0032764 GO:0034101 GO:0034395 GO:0034605 GO:0035094 GO:0035556 GO:0042167 GO:0042168 GO:0042542 GO:0042803 GO:0043123 GO:0043231 GO:0043305 GO:0043392 GO:0043433 GO:0043524 GO:0043619 GO:0043627 GO:0045766 GO:0046872 GO:0048471 GO:0048661 GO:0048662 GO:0051090 GO:0051260 GO:0055072 GO:0055114 GO:0071243 GO:0071276 GO:0071456 GO:0097421 GO:1902042 GO:1904036 GO:1904706
17	0.06	0.271	4.72	0.09	0.40	1z4eA	GO:0008080
18	0.06	0.233	3.71	0.09	0.31	2yv4A	GO:0000166 GO:0003725 GO:0005524 GO:0005737 GO:0008033 GO:0016740 GO:0016779 GO:0061710

Consensus prediction of GO terms

Molecular Function	GO:0003725	GO:0005524	GO:0061710
GO-Score	0.89	0.59	0.59
Biological Processes	GO:0006400	GO:0006448	GO:0070525	GO:0016072	GO:0065008	GO:0022613
GO-Score	0.60	0.60	0.60	0.60	0.60	0.60
Cellular Component	GO:0005829
GO-Score	0.59

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.