I-TASSER results

I-TASSER results for job id Rv1231c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (180 residues)
MSKPFAPRRLYTPRTSRTLAPRLDPEAVGRTTESIARFFGTGRYLLVQTLLVLTWIVLNL
FAVGLRWDPYPFILLNLAFSTQASYAAPLILLAQNRQEKRDRAVFEEDRRRAAQTKADTE
YNARELAALRLAIGEVPTRDYLRHELDSLRALLAELQPTDPDVAQPRVADEAEQHAKKSG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 \| \| \| \| \| \| \| \| \| MSKPFAPRRLYTPRTSRTLAPRLDPEAVGRTTESIARFFGTGRYLLVQTLLVLTWIVLNLFAVGLRWDPYPFILLNLAFSTQASYAAPLILLAQNRQEKRDRAVFEEDRRRAAQTKADTEYNARELAALRLAIGEVPTRDYLRHELDSLRALLAELQPTDPDVAQPRVADEAEQHAKKSG
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHCCC
Conf.Score	987666666656655555554568758999999999986888999999999999999997646678898878999999999999999999998758999999999999999999999999999999999999999999999999999999999999987777777666666665554469
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 \| \| \| \| \| \| \| \| \| MSKPFAPRRLYTPRTSRTLAPRLDPEAVGRTTESIARFFGTGRYLLVQTLLVLTWIVLNLFAVGLRWDPYPFILLNLAFSTQASYAAPLILLAQNRQEKRDRAVFEEDRRRAAQTKADTEYNARELAALRLAIGEVPTRDYLRHELDSLRALLAELQPTDPDVAQPRVADEAEQHAKKSG
Prediction	665545754144444544444624462033104200433333322331333333232311313444312311231233322323341211111243555345442644243035135404402530550455255234463245415504631562465555446664556476546678
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180

                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHCCC
MSKPFAPRRLYTPRTSRTLAPRLDPEAVGRTTESIARFFGTGRYLLVQTLLVLTWIVLNLFAVGLRWDPYPFILLNLAFSTQASYAAPLILLAQNRQEKRDRAVFEEDRRRAAQTKADTEYNARELAALRLAIGEVPTRDYLRHELDSLRALLAELQPTDPDVAQPRVADEAEQHAKKSG

4tkoB3

0.10

0.06

0.59

1.11

dPPAS2

-------------------------------------------------------LHISLSAKK-------LKVESLKKKREALREKLLQVEEKIKLVKLDWERYKSLFQKGLIPRRKFEEVDTNLKVLLHEREYLEKSIQEINTEIKRAKKGIENARNEFKTIEELKK-----------

4tqlA1

0.10

0.06

0.58

1.07

dPPAS2

---------------------------------------------------------------------------YKQMVQELEKARDRMEKLYKEMVELIQKAIELMRKIFQEVKQEVEKAIEEKLLEKLKQIMQEAKQKMEKLLKQLKEEMKKMKEKMEKLLKEMKQRMEEVKKKMDD

5cwjA

0.11

0.10

0.89

0.93

MUSTER

---DSEEEQERIRRILKEARKSGTEESLRQAIEDVAQLAKKSQDSEVLEEAIRVILRIAKESGS--------------EEALRQAIRAVAEIAKEAQDSEVLEEAIRVILRIAKESGSEEALRQAIRAVAEIAKEAQDPRVLEEAIRVIRQIAEESGSEEARRQAERAEEEIRRRAQ---

4mh6A

0.10

0.09

0.86

0.74

dPPAS

-----------------------GTENLYFQSNAIERLLEIKKIRADRADKAVQRQEYRVANVAAELQKAERSVADYHVWRQEEEERRFAKAKQLKELETLRQEIALLREREAELKQRVAEVKVTLEQERTLLKQKQQEALQAHKTKEKFVQLQQQEIAEQSRQQQYQEELEQEEFR---

4ltoA

0.10

0.06

0.56

0.62

wdPPAS

--------------------------------------PGIAWIALLLLVIFYVFAVMGTKLFPEWFG-YPYFVSFILVSSFTVLNLFIGIIIESMQSAHWEAEDAKRIEQEQRAHDERLEMLQLIRDLSSKVDRLERRS----------------------------------------

5cwbA

0.12

1.00

0.87

wMUSTER

RRVAEKVERLKRSGTSEDEIAEEVAREISEVIRTLKESGSSYEVICECVARIVAEIVEALKRSGTSEDEIAEIVARVISEVIRTLKESYEVICECVARIVAEIVEALKRSEDEIAEIVARVISEVIRTLKESSSYEVIKECVQRIVEEIVEALKRSSEDEINEIVRRVKSEVERTLKESG

2ncaA

0.16

0.09

0.61

0.73

wPPAS

---------------------------------------------------MV-W---HIEVSDDEDETHPNI------------TASLFRWRHQARVERMEQFQKEKEELDRGCRECKRKVAECQRKLKELEVA----EGGKAELERLQAEAQQLRKEERSWEQKLEEMRKKEKSMKDG

5cwqA2

0.18

0.11

0.58

1.07

dPPAS2

---------------------------------------------------------------------------SEEAERASEKAQRVLEEARKVSEEAREQGDDEVLALALIAIALAVLALAEVACCRGNKEEAERAYEDARRVEEEARKVKESAEEQGDSEVKRLAEEAEQLAREAR

5cwjA

0.13

0.12

0.87

0.90

PPAS

-------------------DSEEEQERIRRILKEARKSG--TEESLRQAIEDVAQLAKKSQDSEVLEEAIRVILRIAKESEEALRQAIRAVAEIAKEAQDSEVLEEAIRVILRIAKESEEALRQAIRAVAEIAKEAQDPRVLEEAIRVIRQIAEESGSEEARRQAERAEEEIRRRAQ---

3ia7A3

0.18

0.17

0.91

0.72

Env-PPAS

--PGWQPPRPDAPVVSLGNQFNEHPEFFRACAQAFADT--PWHVVAIGGFLDPAVLGPNVEA--HQWIPF-HSVLAHTGAVLEAFAAPLVLVPHFATEAAPSAERVIELGLGSV--LRPDQLE--PASIREAVERLAADSAVRERVRRQRDILSS---GGPARAADEVEAYLGRVAP---

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-2.61

Estimated TM-score = 0.41±0.14

Estimated RMSD = 11.1±4.6Å

Download Model 2

C-score=-2.82

Download Model 3

C-score=-4.53

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	5cwbA	0.878	1.95	0.106	1.000
2	4c0dA	0.653	3.87	0.107	0.922
3	4by6A	0.653	4.13	0.087	0.950
4	3feyA	0.625	3.58	0.070	0.850
5	5cwlA	0.624	3.81	0.154	0.900
6	5t8vA2	0.621	3.96	0.059	0.906
7	4c0eA	0.621	4.32	0.075	0.939
8	5hb1A	0.618	4.44	0.047	0.928
9	3p8cA	0.607	4.38	0.028	0.906
10	1paqA	0.605	3.74	0.093	0.839

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.09	5	2grr1	III	Rep, Mult	71,72,75,76,79
2	0.05	3	3n0gA	MG	Rep, Mult	101,105,152,155
3	0.04	2	1oya0	III	Rep, Mult	70,71,72,119,120,123,124,127,130,134,162,163
4	0.04	2	1h2u0	III	Rep, Mult	82,88,89
5	0.04	2	4wmrA	865	Rep, Mult	70,74,75,96,99,100,103,104,114,117,118,121,122,149
6	0.04	2	4bo0D	FXE	Rep, Mult	90,93,94,97,147,148,151
7	0.02	1	2ql2C	NUC	Rep, Mult	96,100
8	0.02	1	3ahxD	7PE	Rep, Mult	76,77,78,86
9	0.02	1	2io31	III	Rep, Mult	95,98,101,102,105
10	0.02	1	3prqX	CLA	Rep, Mult	46,53
11	0.02	1	2ie40	III	Rep, Mult	16,66,69,72,111,112,113,116,117
12	0.02	1	3c62B	CA	Rep, Mult	118,120
13	0.02	1	1z5s3	III	Rep, Mult	80,84,87
14	0.02	1	3c2mA	MN	Rep, Mult	115,118
15	0.02	1	1z83B	ZN	Rep, Mult	120,171
16	0.02	1	3pyoY	MG	Rep, Mult	91,138
17	0.02	1	2nr9A	PA6	Rep, Mult	56,60
18	0.02	1	1xrmA	III	Rep, Mult	126,129

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.066	1ujkB	0.493	4.23	0.066	0.744	3.4.23.46	78,82,125
2	0.060	1bgaA	0.585	4.31	0.037	0.861	3.2.1.21	NA
3	0.060	1px8A	0.554	3.96	0.060	0.783	3.2.1.37	NA
4	0.060	2e9lA	0.578	4.21	0.093	0.828	3.2.1.21	NA
5	0.060	1h7aA	0.557	4.22	0.067	0.822	1.17.4.2	NA
6	0.060	1qoxM	0.588	4.16	0.056	0.850	3.2.1.21	77
7	0.060	1bwlA	0.575	3.75	0.026	0.817	1.6.99.1	NA
8	0.060	1gowA	0.578	4.46	0.062	0.850	3.2.1.23	NA
9	0.060	1tr1D	0.584	4.33	0.037	0.856	3.2.1.21	NA
10	0.060	3gnoA	0.530	4.85	0.076	0.844	3.2.1.21	76
11	0.060	2zoxA	0.580	4.20	0.093	0.828	3.2.1.21	NA
12	0.060	2bfgA	0.567	3.92	0.053	0.789	3.2.1.37	NA
13	0.060	3cmjA	0.574	4.32	0.050	0.844	3.2.1.21	NA
14	0.060	2jieA	0.584	4.24	0.056	0.844	3.2.1.21	NA
15	0.060	2z1sA	0.585	4.25	0.056	0.844	3.2.1.21	NA
16	0.060	2dgaA	0.575	4.38	0.030	0.867	3.2.1.21	NA
17	0.060	1b1yA	0.561	4.16	0.046	0.811	3.2.1.2	101,125
18	0.060	1np2A	0.571	4.41	0.093	0.856	3.2.1.21	NA
19	0.060	1gonA	0.584	4.34	0.061	0.861	3.2.1.21	129
20	0.060	1qoxA	0.588	4.16	0.056	0.850	3.2.1.21	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.16	0.625	3.58	0.07	0.85	3feyA	GO:0000184 GO:0000339 GO:0000398 GO:0003723 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005829 GO:0005845 GO:0005846 GO:0006366 GO:0006368 GO:0006369 GO:0006370 GO:0006397 GO:0006405 GO:0006406 GO:0006417 GO:0006446 GO:0006810 GO:0008334 GO:0008380 GO:0008543 GO:0010467 GO:0016070 GO:0030307 GO:0030529 GO:0031047 GO:0031124 GO:0031442 GO:0034518 GO:0042795 GO:0044822 GO:0045292 GO:0051028 GO:0051168 GO:0098789 GO:1900363
1	0.09	0.651	3.83	0.11	0.91	5fu7A	GO:0000122 GO:0000289 GO:0000932 GO:0004535 GO:0005615 GO:0005634 GO:0005737 GO:0005778 GO:0005829 GO:0006351 GO:0006355 GO:0006417 GO:0006977 GO:0007275 GO:0010606 GO:0016020 GO:0017148 GO:0019904 GO:0030014 GO:0030015 GO:0030331 GO:0031047 GO:0032947 GO:0033147 GO:0035195 GO:0042974 GO:0043231 GO:0044822 GO:0048387 GO:0060213 GO:0061014 GO:0070016 GO:0090503 GO:1900153 GO:2000036
2	0.08	0.653	4.13	0.09	0.95	4by6A	GO:0046872
3	0.07	0.630	3.48	0.08	0.85	1h2uA	GO:0000184 GO:0000339 GO:0000398 GO:0003723 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005829 GO:0005845 GO:0005846 GO:0006366 GO:0006368 GO:0006369 GO:0006370 GO:0006397 GO:0006405 GO:0006406 GO:0006417 GO:0006446 GO:0006810 GO:0008334 GO:0008380 GO:0008543 GO:0010467 GO:0016070 GO:0030307 GO:0030529 GO:0031047 GO:0031124 GO:0031442 GO:0034518 GO:0042795 GO:0044822 GO:0045292 GO:0051028 GO:0051168 GO:0098789 GO:1900363
4	0.07	0.514	4.52	0.06	0.78	4d0jA	GO:0000272 GO:0004553 GO:0005975 GO:0008152 GO:0008422 GO:0016787 GO:0016798 GO:0030245 GO:0102483
5	0.06	0.403	5.01	0.07	0.66	1z7aA	GO:0003824 GO:0005975 GO:0016810
6	0.06	0.427	4.51	0.03	0.66	2xn0A	GO:0003824 GO:0004553 GO:0004557 GO:0005975 GO:0008152 GO:0016787 GO:0016798 GO:0052692
7	0.06	0.364	4.55	0.07	0.57	1v4vA	GO:0008761 GO:0016853
8	0.06	0.396	5.22	0.04	0.66	3wpnA	GO:0000166 GO:0000922 GO:0003777 GO:0005524 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005874 GO:0005876 GO:0006890 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0007067 GO:0007100 GO:0008017 GO:0008574 GO:0016020 GO:0019886 GO:0019901 GO:0032403 GO:0046602 GO:0051225 GO:0051301 GO:0090307
9	0.06	0.343	5.21	0.06	0.58	4wjiA	GO:0000166 GO:0004665 GO:0006571 GO:0008977 GO:0055114
10	0.06	0.309	5.80	0.03	0.57	4p3lA	GO:0006810 GO:0008643 GO:0030288 GO:0042597
11	0.06	0.306	6.22	0.04	0.60	3l4dB	GO:0004497 GO:0005506 GO:0008168 GO:0008398 GO:0016491 GO:0016705 GO:0020037 GO:0032259 GO:0046872 GO:0055114 GO:0070988
12	0.06	0.316	5.26	0.12	0.52	1y97A	GO:0000287 GO:0003676 GO:0004518 GO:0004527 GO:0005634 GO:0006259 GO:0006281 GO:0006974 GO:0008296 GO:0008853 GO:0016787 GO:0042803 GO:0046872 GO:0090305
13	0.06	0.455	4.78	0.07	0.74	2bklB	GO:0004252 GO:0006508 GO:0008236 GO:0070008
14	0.06	0.272	5.37	0.06	0.44	4gxzA	GO:0005623 GO:0015035 GO:0045454 GO:0055114
15	0.06	0.273	4.02	0.01	0.39	2ktbB	GO:0000228 GO:0003677 GO:0003682 GO:0005634 GO:0005654 GO:0006338 GO:0006351 GO:0006355 GO:0007067 GO:0008285 GO:0016568 GO:0090544
16	0.06	0.317	5.37	0.05	0.53	3iypB	GO:0000166 GO:0001172 GO:0003723 GO:0003724 GO:0003968 GO:0004197 GO:0004386 GO:0005198 GO:0005216 GO:0005524 GO:0006351 GO:0006508 GO:0006810 GO:0006811 GO:0008233 GO:0008234 GO:0016020 GO:0016032 GO:0016740 GO:0016779 GO:0016787 GO:0017111 GO:0018144 GO:0019012 GO:0019028 GO:0019062 GO:0030430 GO:0033644 GO:0034220 GO:0039694 GO:0039707 GO:0044161 GO:0044162 GO:0044385 GO:0046718 GO:0051259
17	0.06	0.248	4.61	0.02	0.38	3idoA	GO:0004725 GO:0006470 GO:0035335
18	0.06	0.254	3.47	0.06	0.34	1qsdA	GO:0005737 GO:0005856 GO:0005874 GO:0007021 GO:0007023 GO:0015631 GO:0048487

Consensus prediction of GO terms

Molecular Function	GO:0003723
GO-Score	0.57
Biological Processes	GO:0034248	GO:2000112	GO:0032268	GO:0016458	GO:0010608	GO:0006366	GO:0098787	GO:0045927	GO:0000956	GO:0006413	GO:0006354	GO:0001558	GO:0009301	GO:0050685	GO:0071427	GO:0031440	GO:0006405	GO:0006378	GO:0007169	GO:0000377	GO:0009452	GO:0006353	GO:0044344
GO-Score	0.57	0.57	0.57	0.57	0.57	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44	0.44
Cellular Component	GO:0044444	GO:1990904	GO:0034518	GO:0031981
GO-Score	0.57	0.44	0.44	0.44

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.