I-TASSER results

I-TASSER results for job id Rv1219c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (212 residues)
MRSADLTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDF
VAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNA
EEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVL
PSLEVYTEGLLADRAMYEAFLAEAQQGEAHVG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 \| \| \| \| \| \| \| \| \| \| MRSADLTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAHVG
Prediction	CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
Conf.Score	98568999999999999999997877889999999899998988888999999999999999999999988756899799999999866556999999999757989999999999999999999999989999999999999999999999999998657887877899999999999999999985888996888888876567887789
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 \| \| \| \| \| \| \| \| \| \| MRSADLTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAHVG
Prediction	84576362143014002510164212020430064171343102211431530032004301540353155226644224213202313523410210130035344103301441243024003301543533663214000100012121111011114452441352035103300320031024211334311442255457565668
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
MRSADLTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAHVG

4nn1A

1.00

0.97

2.31

MUSTER

-----LTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAH--

4nn1A

1.00

0.97

2.05

dPPAS

-----LTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAH--

4nn1A

1.00

0.97

2.62

wdPPAS

-----LTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAH--

4nn1A

1.00

0.97

2.92

wMUSTER

-----LTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAH--

4nn1A

1.00

0.97

3.29

wPPAS

-----LTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAH--

4nn1A

1.00

0.97

2.95

dPPAS2

-----LTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAH--

4nn1A

1.00

0.97

3.04

PPAS

-----LTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAH--

4nn1A

1.00

0.97

3.36

Env-PPAS

-----LTAHARIREAAIEQFGRHGFGVGLRAIAEAAGVSAALVIHHFGSKEGLRKACDDFVAEEIRSSKAAALKSNDPTTWLAQMAEIESYAPLMAYLVRSMQSGGELAKMLWQKMIDNAEEYLDEGVRAGTVKPSRDPRARARFLAITGGGGFLLYLQMHENPTDLRAALRDYAHDMVLPSLEVYTEGLLADRAMYEAFLAEAQQGEAH--

2w53A

0.17

0.16

0.92

1.66

MUSTER

--EDTQATREGILDAAEACFHEHGVRTTLEMIGARAGYTRGAVYWHFKNKSEVLAAIVERVHLPFMQELERTSQRDTPVHDLRAVMIHSFIDERLRKTMEIMLRSTEMQQAGFRDALDRMERALRRARDLGQLREGADPKIAARMLHATVLGVLHGAMV-EPELMDLKRDGMLALDMTLAAYVK---DGVFVPGTV-------PEPLP----

2w53A

0.17

0.16

0.92

1.39

dPPAS

--EDTQATREGILDAAEACFHEHGVRTTLEMIGARAGYTRGAVYWHFKNKSEVLAAIVERVHLPFMQELERTSTDQRLRAVMIHSFIELSEDERLRKTMEIMLRSTEMQQAGFRDALDRMERALRRARDLGQLREGADPKIAARMLHATVLGVLHGAMVEPELMD-----LKRDGMLALDMTLAAYVKDGVFVPGT-------VPEPLP---

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=1.02

Estimated TM-score = 0.85±0.08

Estimated RMSD = 3.4±2.4Å

Download Model 2

C-score=-3.31

Download Model 3

C-score=-4.01

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4nn1A	0.951	0.71	1.000	0.967
2	3e7qB	0.677	3.65	0.186	0.887
3	3lsjA	0.669	3.43	0.139	0.873
4	3bhqA	0.666	3.67	0.148	0.854
5	2qibA	0.656	3.44	0.135	0.849
6	3bcgA	0.642	3.97	0.132	0.891
7	2g3bB	0.641	3.41	0.168	0.807
8	3vibA	0.640	3.67	0.166	0.854
9	2uxuA	0.638	3.84	0.188	0.877
10	3eupA	0.638	3.74	0.152	0.849

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.25	15	4l62A	NUC	Rep, Mult	27,28,29,40,44,49,50
2	0.14	9	4i6zB	NUC	Rep, Mult	8,38,39,41,42,45,46
3	0.09	7	3p9tA	TCL	Rep, Mult	63,101,102,112,113,116,120,146,147,150,154
4	0.05	4	3lsrA	QNA	Rep, Mult	28,29,30,34,40,41,44
5	0.01	1	2a68F	MG	Rep, Mult	12,13,47
6	0.01	1	3p9tA	TCL	Rep, Mult	58,62,65,98,101
7	0.01	1	3p9tA	TCL	Rep, Mult	62,65,78,98,99,112,113,116,120,146,147,150,154
8	0.01	1	3locA	URA	Rep, Mult	156,159
9	0.01	1	3iuv0	III	Rep, Mult	25,26,113,142,144,145,148,151,152,155,158,159,161,181
10	0.01	1	2np5C	NDS	Rep, Mult	102,103,105,109
11	0.01	1	3p9tA	TCL	Rep, Mult	58,62,65,85,95,98

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2ewnA	0.422	5.36	0.065	0.708	6.3.4.15	NA
2	0.060	1bf2A	0.429	5.84	0.070	0.745	3.2.1.68	NA
3	0.060	1h0nA	0.421	4.76	0.064	0.637	1.17.4.1	97
4	0.060	2uxwA	0.435	5.39	0.062	0.731	1.3.99.-	127
5	0.060	2vuxB	0.388	4.54	0.061	0.585	1.17.4.1	123
6	0.060	2ztgA	0.446	5.00	0.061	0.708	6.1.1.7	9
7	0.060	1y8bA	0.438	4.86	0.053	0.674	2.3.3.9	NA
8	0.060	1d8cA	0.449	4.81	0.053	0.684	2.3.3.9	29
9	0.060	1w07B	0.452	4.98	0.048	0.693	1.3.3.6	NA
10	0.060	2zzgA	0.450	5.27	0.043	0.741	6.1.1.7	NA
11	0.060	2pywA	0.468	4.12	0.080	0.646	2.7.1.100	NA
12	0.060	1cqzB	0.412	5.54	0.079	0.703	3.3.2.10,3.3.2.3	NA
13	0.060	1y791	0.448	5.07	0.073	0.693	3.4.15.5	97
14	0.060	1biqB	0.421	4.92	0.063	0.646	1.17.4.1	NA
15	0.060	1bibA	0.423	5.63	0.071	0.726	6.3.4.15	NA
16	0.060	1rsrB	0.441	4.64	0.057	0.646	1.17.4.1	NA
17	0.060	2ivfA	0.427	5.33	0.029	0.703	1.17.99.2	NA
18	0.060	3djlA	0.424	4.97	0.056	0.656	1.3.99.-	NA
19	0.060	2i2xA	0.451	5.03	0.036	0.717	2.1.1.90	20,24

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.71	0.951	0.71	1.00	0.97	4nn1A	GO:0003677 GO:0003700 GO:0006351 GO:0006355
1	0.32	0.628	3.44	0.17	0.82	4l62A	GO:0000976 GO:0003677 GO:0003700 GO:0005829 GO:0006351 GO:0006355
2	0.30	0.551	3.95	0.12	0.72	2zozB	GO:0003677 GO:0006351 GO:0006355
3	0.30	0.638	3.71	0.16	0.83	2np5A	GO:0003677 GO:0006351 GO:0006355
4	0.29	0.677	3.65	0.19	0.89	3e7qB	GO:0003677 GO:0003700 GO:0006351 GO:0006355
5	0.28	0.645	3.71	0.21	0.85	2id6A	GO:0003677 GO:0006351 GO:0006355
6	0.28	0.641	3.91	0.17	0.88	3vibC	GO:0003677 GO:0006351 GO:0006355
7	0.28	0.605	3.38	0.17	0.77	4aciB	GO:0000287 GO:0003677 GO:0006351 GO:0006355 GO:0046872
8	0.27	0.575	3.59	0.21	0.74	2qwtA	GO:0003677 GO:0006351 GO:0006355
9	0.27	0.612	4.03	0.16	0.83	3f1bA	GO:0003677 GO:0006351 GO:0006355
10	0.25	0.603	4.07	0.17	0.83	3kz9B	GO:0003677 GO:0006351 GO:0006355
11	0.25	0.671	3.39	0.19	0.83	2g3bA	GO:0003677 GO:0006351 GO:0006355
12	0.24	0.647	3.58	0.18	0.85	3qbmA	GO:0003677 GO:0003700 GO:0006351 GO:0006355
13	0.23	0.483	3.81	0.13	0.66	3ljlA	GO:0003677
14	0.23	0.593	3.59	0.14	0.77	2q24B	GO:0003677 GO:0006351 GO:0006355
15	0.23	0.570	4.27	0.16	0.80	3vprA	GO:0003677 GO:0006351 GO:0006355 GO:0042802
16	0.23	0.622	3.88	0.15	0.83	2pbxA
17	0.21	0.564	3.95	0.15	0.76	3egqA	GO:0003677 GO:0006351 GO:0006355
18	0.21	0.619	3.67	0.14	0.83	3on4A	GO:0003677 GO:0006351 GO:0006355
19	0.21	0.562	4.45	0.13	0.82	3ccyA	GO:0003677 GO:0006351 GO:0006355
20	0.21	0.571	4.11	0.20	0.79	3cdlB	GO:0003677 GO:0006351 GO:0006355
21	0.21	0.575	4.00	0.17	0.81	3jsjA	GO:0003677 GO:0006351 GO:0006355
22	0.21	0.539	4.70	0.12	0.80	4me9A	GO:0003677 GO:0006351 GO:0006355
23	0.21	0.628	3.94	0.17	0.85	4jykA	GO:0000976 GO:0003677 GO:0003700 GO:0005829 GO:0006351 GO:0006355 GO:0045892 GO:0045893
24	0.20	0.518	4.81	0.16	0.81	3dewA	GO:0003677 GO:0006351 GO:0006355 GO:0016020 GO:0016021
25	0.19	0.540	4.05	0.14	0.75	2np3B	GO:0003677 GO:0006351 GO:0006355
26	0.19	0.664	3.56	0.15	0.86	2qibA	GO:0003677 GO:0003700 GO:0006351 GO:0006355
27	0.19	0.606	4.11	0.13	0.82	2genA	GO:0003677 GO:0003700 GO:0006351 GO:0006355
28	0.19	0.615	4.19	0.16	0.85	2qtqA	GO:0003677 GO:0006351 GO:0006355
29	0.17	0.597	4.17	0.18	0.83	3ppbA	GO:0003677 GO:0006351 GO:0006355
30	0.17	0.574	4.17	0.14	0.78	2pz9A	GO:0003677 GO:0003700 GO:0006351 GO:0006355
31	0.16	0.554	4.53	0.11	0.81	5d1wD	GO:0003677 GO:0006351 GO:0006355
32	0.16	0.534	4.42	0.15	0.76	3gziA	GO:0003677 GO:0006351 GO:0006355
33	0.15	0.659	4.07	0.14	0.89	3npiA	GO:0003677 GO:0006351 GO:0006355
34	0.15	0.602	3.65	0.14	0.82	2hkuA	GO:0003677 GO:0006351 GO:0006355
35	0.11	0.555	4.42	0.13	0.80	4w97A	GO:0003677 GO:0003700 GO:0006351 GO:0006355 GO:0010468 GO:0019217
36	0.11	0.549	4.53	0.13	0.78	4mxmA	GO:0003677 GO:0006351 GO:0006355
37	0.10	0.635	3.87	0.18	0.88	2uxhA	GO:0003677 GO:0006351 GO:0006355
38	0.09	0.515	4.76	0.15	0.78	2ibdA	GO:0003677 GO:0006351 GO:0006355
39	0.09	0.642	4.16	0.14	0.91	3c07A	GO:0003677 GO:0006351 GO:0006355
40	0.08	0.591	3.85	0.19	0.80	2guhA	GO:0003677 GO:0006351 GO:0006355
41	0.07	0.560	3.97	0.15	0.79	2raeA	GO:0003677 GO:0006351 GO:0006355
42	0.07	0.612	4.36	0.18	0.88	1t33A	GO:0003677 GO:0003700 GO:0006351 GO:0006355
43	0.07	0.605	3.69	0.17	0.80	3knwA	GO:0003677 GO:0006351 GO:0006355
44	0.07	0.532	4.53	0.13	0.81	2dg7A	GO:0003677 GO:0006351 GO:0006355

Consensus prediction of GO terms

Molecular Function	GO:0003700	GO:0000976
GO-Score	0.86	0.32
Biological Processes	GO:0006355
GO-Score	0.93
Cellular Component	GO:0005829
GO-Score	0.32

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.