I-TASSER results

I-TASSER results for job id Rv1215c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (561 residues)
MARNPSPALDRPWRRPGALRYALERVRGVAKPPITVTDPPADVVIERDVEVPTRDGTLLR
INVFRSAEGGARPVIASIHPYGKDALPRRRGNRWTFSPQYRMLRQPKPLTFSALTGWEAP
DPAWWTAQGFVVVNADSRGCGRSDGTGDLLSHQEAEDTYDLVGWLADQAWSDGRVVMLGV
SYLAISQYAVAALQPPALRAICPWEGFTDAYRDLAFPGGIRESGFTRLWSRGVRRRTRQT
YDMEQMQEAHPLRDDFWRSRVPDLSAIKVPMLVCGSFSDNNLHSRGSIRAFTRSGCGHAR
LYTHRGGKWETFYSATALSEQLKFLRDALAGSSGSRSVRLEVREDRDTITAVREETQWPL
AGTRWRPMYLAGPGLLATEPPPTAGSIRFQTRSRAAAFNWTIPEDIELTGPMAARLWVQL
DGCDDANLFVGVEKWRDGQFVAFEGSYGWGRDRVTTGWQRVSLRELDPELSQPWEPVPAC
ARPRPVTAGEVVAVDVALGPSATLFRAGEQLRLVVGGRWLSPRNPLTGQFPAAYPRPPRG
RVTLHWGPRYDAHLLIPEVPG

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MARNPSPALDRPWRRPGALRYALERVRGVAKPPITVTDPPADVVIERDVEVPTRDGTLLRINVFRSAEGGARPVIASIHPYGKDALPRRRGNRWTFSPQYRMLRQPKPLTFSALTGWEAPDPAWWTAQGFVVVNADSRGCGRSDGTGDLLSHQEAEDTYDLVGWLADQAWSDGRVVMLGVSYLAISQYAVAALQPPALRAICPWEGFTDAYRDLAFPGGIRESGFTRLWSRGVRRRTRQTYDMEQMQEAHPLRDDFWRSRVPDLSAIKVPMLVCGSFSDNNLHSRGSIRAFTRSGCGHARLYTHRGGKWETFYSATALSEQLKFLRDALAGSSGSRSVRLEVREDRDTITAVREETQWPLAGTRWRPMYLAGPGLLATEPPPTAGSIRFQTRSRAAAFNWTIPEDIELTGPMAARLWVQLDGCDDANLFVGVEKWRDGQFVAFEGSYGWGRDRVTTGWQRVSLRELDPELSQPWEPVPACARPRPVTAGEVVAVDVALGPSATLFRAGEQLRLVVGGRWLSPRNPLTGQFPAAYPRPPRGRVTLHWGPRYDAHLLIPEVPG
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEHCCCCCEEEEEEEHCCCCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHCCCHHHHHHHHHHHCCCCCEEEEEEHCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEHCCCCCCCCCHHHHHHHHHHCCCCCEEEEHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHCCCCCCEEEHCCCCCCCCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCEEHCCCCCCCEEEHCCEEEEEEEEHCCCCCCEEEEEEEEHCCCCEEHCCCCCCCCCCCCCEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEHCCEEEEHCCCEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHCCCCCCEEEEHCCCC
Conf.Score	998888887777678877765555666666788778888987789988888778985999999978999986599998688988877666666667776655556777777766666677778888879789997789888888887679999999999999999989998995898666778999999998599866899864776665555456798888764689999888765667788999998788996788776777657898899857788877756799999986888866898567889899876999999999999986988888777669985678765786577999998578888579987678899888875556788874765888998799857699999985899865899999985799677656777776656655689987534675567788888888876568999789999998565888589869999998678888888888888777777775899997899986899975899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MARNPSPALDRPWRRPGALRYALERVRGVAKPPITVTDPPADVVIERDVEVPTRDGTLLRINVFRSAEGGARPVIASIHPYGKDALPRRRGNRWTFSPQYRMLRQPKPLTFSALTGWEAPDPAWWTAQGFVVVNADSRGCGRSDGTGDLLSHQEAEDTYDLVGWLADQAWSDGRVVMLGVSYLAISQYAVAALQPPALRAICPWEGFTDAYRDLAFPGGIRESGFTRLWSRGVRRRTRQTYDMEQMQEAHPLRDDFWRSRVPDLSAIKVPMLVCGSFSDNNLHSRGSIRAFTRSGCGHARLYTHRGGKWETFYSATALSEQLKFLRDALAGSSGSRSVRLEVREDRDTITAVREETQWPLAGTRWRPMYLAGPGLLATEPPPTAGSIRFQTRSRAAAFNWTIPEDIELTGPMAARLWVQLDGCDDANLFVGVEKWRDGQFVAFEGSYGWGRDRVTTGWQRVSLRELDPELSQPWEPVPACARPRPVTAGEVVAVDVALGPSATLFRAGEQLRLVVGGRWLSPRNPLTGQFPAAYPRPPRGRVTLHWGPRYDAHLLIPEVPG
Prediction	654545343342143242232103313123322231441324011235040202340323010000357421000000000112223334132231222221123122222132221312202100300000000000000102021131243003001000100161320101000000000000000000121410000000000000000100000000010010101002222311110220121011100002001220320100000000000000111001200230344412000020000000112200210031023102225342302010000133323113153012662531301013623034432544443314153311000110431010112020102001022000000000011254330213313412422001000000003214633342221211432330425430504030100012043101000000001012211112322242443430201011036100000000218
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEHCCCCCEEEEEEEHCCCCCCCEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHCCCHHHHHHHHHHHCCCCCEEEEEEHCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEHCCCCCCCCCHHHHHHHHHHCCCCCEEEEHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHCCCCCCEEEHCCCCCCCCCEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCEEHCCCCCCCEEEHCCEEEEEEEEHCCCCCCEEEEEEEEHCCCCEEHCCCCCCCCCCCCCEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEHCCEEEEHCCCEEEEEEEHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHCCCCCCEEEEHCCCC
MARNPSPALDRPWRRPGALRYALERVRGVAKPPITVTDPPADVVIERDVEVPTRDGTLLRINVFRSAEGGARPVIASIHPYGKDALPRRRGNRWTFSPQYRMLRQPKPLTFSALTGWEAPDPAWWTAQGFVVVNADSRGCGRSDGTGDLLSHQEAEDTYDLVGWLADQAWSDGRVVMLGVSYLAISQYAVAALQPPALRAICPWEGFTDAYRDLAFPGGIRESGFTRLWSRGVRRRTRQTYDMEQMQEAHPLRDDFWRSRVPDLSAIKVPMLVCGSFSDNNLHSRGSIRAFTRSGCGHARLYTHRGGKWETFYSATALSEQLKFLRDALAGSSGSRSVRLEVREDRDTITAVREETQWPLAGTRWRPMYLAGPGLLATEPPPTAGSIRFQTRSRAAAFNWTIPEDIELTGPMAARLWVQLDGCDDANLFVGVEKWRDGQFVAFEGSYGWGRDRVTTGWQRVSLRELDPELSQPWEPVPACARPRPVTAGEVVAVDVALGPSATLFRAGEQLRLVVGGRWLSPRNPLTGQFPAAYPRPPRGRVTLHWGPRYDAHLLIPEVPG

3iiiA

0.32

0.30

0.95

3.03

MUSTER

--QHLLGNPKLTVTHVNEVKAGINH--------IVVDSVQYGNQEIEKDGTVERDGEKLYINIFRPNKDGKFPVVSAD-TYGKDNKPKNGAL----------WPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAIPWE-GLND-YREVAFHGGIPDTGFYRFWTQGIFARWTDNEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEQFYKGEFKSASAFPLPNAEYTPLYLNAENHLNHAKISSAHVAQYDSEDQDVSFKYTFDKDTELVGN-NLKLWVSTKDSDDDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGKTRYEHEE-TVNKGHIYTGGKYDSQLIIPIVN-

3iiiA

0.30

0.29

0.95

3.88

dPPAS

----------QHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEIEKDGTVERDGEKLYINIFRPNKDGKFPVVSADT-----------YGKDNKPKNGALWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAIPWEGLND--YREVAFHGGIPDTGFYRFWTQGIFARWTDNEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAQYDSEDKQQDVSFKYTFDKDTELVGN-NLKLWVSTKDSDDDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGKTRYEHEETVN-KGHIYTGGKYDSQLIIPIVN-

3iiiA

0.31

0.29

0.95

5.29

wdPPAS

-------------QHLLGNPKLTVTHVNEVKAHIVVDSVQYGNQEIEKDGTVERDGEKLYINIFRPNKDGKFPVVSADTYGKDNKPKNGA-----------LWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAIPWEGLND--YREVAFHGGIPDTGFYRFWTQGIFARWTDNPDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAQYDSEDKQQDVSFKYTFDKDTELVGN-NLKLWVSTKDSDDDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGKTRYEH-EETVNKGHIYTGGKYDSQLIIPIVN-

3iiiA

0.31

0.30

0.95

4.02

wMUSTER

LLGNPKLTVTHVNEVKAGINHIV------------VDSVQYGNQEIEKDGTVERDGEKLYINIFRPNKDGKFPVVSADTYGKDNKPKNGA-----------LWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAIPWE-GLND-YREVAFHGGIPDTGFYRFWTQGIFARWTDNEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEVFYKGEFKSASAFPLPNAEYTPLYLNAENHLNHAKISSAHVAQYDSEDKDVSFKYTFDKDTELVGN-NLKLWVSTKDSDDDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGKTRYEHEE-TVNKGHIYTGGKYDSQLIIPIVN-

3iiiA

0.31

0.29

0.95

5.83

wPPAS

LGNPKLTVTHVNEVKAGINHIV-------------VDSVQYGNQEIEKDGTVERDGEKLYINIFRPNKDGKFPVVSADTYGKDNKPKNGAL-----------WPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAIPWE-GLND-YREVAFHGGIPDTGFYRFWTQGIFARWTDNEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAQYDSEDKQQDVSFKYTFDKDTELVGN-NLKLWVSTKDSDDDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGKTRYE-HEETVNKGHIYTGGKYDSQLIIPIVN-

3iiiA

0.32

0.30

0.95

6.90

dPPAS2

-------------QHLLGNPKLTVTHVNEVKAHIVVDSVQYGNQEIEKDGTVERDGEKLYINIFRPNKDGKFPVVSAD-TYGKDNKPKN----------GALWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAIPWEGLND--YREVAFHGGIPDTGFYRFWTQGIFARWTDNEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAQYDSEDKQQDVSFKYTFDKDTELVGN-NLKLWVSTKDSDDDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGKTRYEHEE-TVNKGHIYTGGKYDSQLIIPIVN-

3iiiA

0.32

0.30

0.95

5.19

PPAS

-----QHLLGNPKLTVTHVNEVKAGINH-----IVVDSVQYGNQEIEKDGTVERDGEKLYINIFRPNKDGKFPVVSAD-TYGKDNKPKNGALWP----------TLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAIPWE-GLND-YREVAFHGGIPDTGFYRFWTQGIFARWTDNEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAQYDSEDKQQDVSFKYTFDKDTELVGN-NLKLWVSTKDSDDDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGKTRY-EHEETVNKGHIYTGGKYDSQLIIPIVN-

3iiiA

0.32

0.31

0.95

6.74

Env-PPAS

-----QHLLGNPKLTVTHVNEVKAGINH-----IVVDSVQYGNQEIEKDGTVERDGEKLYINIFRPNKDGKFPVVSAD-TYGKDNKPKNGALWPTL----------GTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAIPWE-GLND-YREVAFHGGIPDTGFYRFWTQGIFARWTDNEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAQYDSEDKQQDVSFKYTFDKDTELVGN-NLKLWVSTKDSDDDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGKTRYEHEE-TVNKGHIYTGGKYDSQLIIPIVN-

4pf1A

0.20

0.18

0.89

2.31

MUSTER

----------------------FSEDSDSDIPEKFTPKTDLFDYTRREEMIPMRDGVKLNTIILIPKGVQNTPIVLTRTPYHAERRTLRF-------------------NSSSLSMVVPQMNDTTSAARYIIVYQDVRGKYGSEGGYMMNKPDHSTDTYDTIDWLVKNPESNGRVAAIGGSYEGYTTLMCTINPHPALKAVVPFASMVDGWDDWFHMGAFRQEASLPYAYNQEATRKNEIKWFWKKLAAHPSYDSFWQQMDKMLAQLTVPMLIVGGLFDQE-DIYGSPKLYKVLAGKLVHFVLGPWNHGQGRRDWFRRNVMQPFLDHYLKD--APKLDIPRVLSYETGANAWHRYDDWPPEEAHYCDLYVQEDGKLGFEMPAAKQAFDYRQRPTIVWATEPLKEPLRVAGQPVARLFASTSGSDADWVVKIIDVWPDEVP--ENPKLGGYQQMLSADIFRGRYRE-------------DFAVAKPLVPDKVLEYRIPLPQVSHTFLPGHRIMVQVQSSWFPDRNPQTFVPNAPPESYRKATQRVWRTAEYPTAIEIHIIS-

3idaA

0.25

0.21

0.83

2.82

dPPAS

----------------------------------------GNYSVASNVMVPMRDGVRLAVDLYRPDADGPVPVLLVRNPYDKFDVFAW--------------------------STQSTNWLEFVRDGYAVVIQDTRGLFASEGEFVPHV-DDEADAEDTLSWILEQAWCDGNVGMFGVSYLGVTQWQAAVSGVGGLKAIAPSMASADLYRAWYGPGGALSVEALLGWSAKIGTQLITSRSVIDQVVDHPDNDESWQSIFERLGGLATPALITAGWYDGFV--GESLRTFVAVKDNDARLVVGPWSHAATYPIQEATTMHKAFFDRHLRGETDALAGVPKVRLFVMGIDEWRDETDWPLPDTAYTPFYLGGSGAANTSTGGLGGTLLFHNGDNGCYSTEVLTDPVEVTGTVSARLFVSSSAVDTDFTAKLVDVFPDGRAIA-----------LCDGIVRMRYR-------------ETLVNPTLIEAGEIYEVAIDMLATSNVFLPGHRIMVQVSSSNFPKYDRNSNTGGEQLEEMCTAVNRIHRGPEHPSHIVLPIIKR

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 3 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=0.50

Estimated TM-score = 0.78±0.10

Estimated RMSD = 6.5±3.9Å

Download Model 2

C-score=0.41

Download Model 3

C-score=0.43

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	3iiiA	0.920	1.75	0.328	0.952
2	3idaA	0.757	2.45	0.261	0.813
3	1nx9A	0.755	3.58	0.204	0.850
4	1mpxA	0.739	3.13	0.171	0.822
5	1lnsA2	0.739	3.39	0.142	0.824
6	2veoA	0.441	4.54	0.122	0.535
7	1aknA	0.431	5.29	0.098	0.545
8	3i6mA	0.427	4.75	0.101	0.522
9	3g0bB	0.425	4.02	0.117	0.495
10	1qo9A	0.425	5.01	0.108	0.526

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.24	10	2b4kA	PG9	Rep, Mult	81,181,182,207,214,215,229,282,283,308
2	0.08	3	3i2gA	DBC	Rep, Mult	81,150,181,182,214,229,283,308,388,389
3	0.05	2	1u8eB	NAG	Rep, Mult	55,138,157,160
4	0.05	2	3idaA	DBC	Rep, Mult	81,181,182,229
5	0.05	2	1u8eA	UUU	Rep, Mult	63,65,130,132,133,134
6	0.02	1	4bzzA	ACT	Rep, Mult	124,128,322
7	0.02	1	4c01A	QY9	Rep, Mult	50,164,167,168

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.139	1l7qA	0.760	2.38	0.257	0.813	3.1.1.1	NA
2	0.060	1nx9A	0.755	3.58	0.204	0.850	3.1.1.43	52,58
3	0.060	1b41A	0.425	5.15	0.113	0.531	3.1.1.7	NA
4	0.060	2b4kA	0.755	3.58	0.206	0.850	3.1.1.43	181,182,279
5	0.060	1mpxA	0.739	3.13	0.171	0.822	3.2.1.43	NA
6	0.060	1aknA	0.431	5.29	0.098	0.545	3.1.1.13,3.1.1.3	NA
7	0.060	2jbwD	0.428	3.47	0.150	0.488	3.7.1.-	181
8	0.060	1k8qA	0.408	4.28	0.108	0.481	3.1.1.3	NA
9	0.060	2d5lA	0.404	4.18	0.133	0.472	3.4.14.-	181,279
10	0.060	1l7aA	0.393	3.12	0.130	0.439	3.1.1.72,3.1.1.41	172
11	0.060	2fj0A	0.422	5.38	0.093	0.533	3.1.1.1	278
12	0.060	1mx9D	0.416	5.15	0.131	0.520	3.1.1.1	NA
13	0.060	3fvrA	0.395	3.30	0.126	0.444	3.1.1.6	181,279
14	0.060	1e5tA	0.425	4.22	0.119	0.497	3.4.21.26	NA
15	0.060	1hlgA	0.406	4.46	0.105	0.487	3.1.1.3	553
16	0.060	2veoB	0.439	4.60	0.125	0.535	3.1.1.3	139
17	0.060	3g0bB	0.425	4.02	0.117	0.495	3.4.14.5	137
18	0.060	1z68A	0.420	4.10	0.123	0.490	3.4.21.-	181,279
19	0.060	1c7jA	0.403	4.88	0.129	0.492	3.1.1.-	119
20	0.060	1yr2A	0.420	4.30	0.141	0.492	3.4.21.26	181,279

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.23	0.933	1.67	0.33	0.96	3iiiA	GO:0006508 GO:0008239 GO:0016787
1	0.23	0.762	2.40	0.26	0.82	3i2gA	GO:0005737 GO:0006508 GO:0008239 GO:0016787 GO:0050784 GO:0052689
2	0.22	0.755	3.58	0.21	0.85	2b4kA	GO:0006508 GO:0008239 GO:0016787 GO:0047658
3	0.14	0.760	3.15	0.19	0.84	4pf1A	GO:0006508 GO:0008239 GO:0016787
4	0.13	0.756	3.35	0.18	0.84	1mpxA	GO:0006508 GO:0008239 GO:0016787 GO:0046872 GO:0047658
5	0.13	0.737	3.50	0.14	0.83	1lnsA	GO:0004177 GO:0005737 GO:0006508 GO:0008233 GO:0008236 GO:0008239 GO:0016787
6	0.06	0.371	3.66	0.11	0.42	3aikA	GO:0008152 GO:0016787 GO:0042802 GO:0052689
7	0.06	0.326	3.39	0.14	0.37	2i3dB	GO:0016787
8	0.06	0.251	7.08	0.04	0.39	5hdrC	GO:0004560 GO:0005975 GO:0006004
9	0.06	0.255	7.44	0.05	0.41	4peeC	GO:0004560 GO:0005975 GO:0006004
10	0.06	0.257	7.54	0.03	0.42	2panA	GO:0000287 GO:0003824 GO:0009028 GO:0009436 GO:0016829 GO:0030976 GO:0042802 GO:0046296 GO:0071949
11	0.06	0.242	7.40	0.05	0.39	1m7sA	GO:0004096 GO:0004601 GO:0006979 GO:0016491 GO:0020037 GO:0042597 GO:0042744 GO:0046872 GO:0055114 GO:0098869
12	0.06	0.210	6.67	0.04	0.31	3hurA	GO:0003824 GO:0006522 GO:0008784 GO:0016853 GO:0030170 GO:0030632
13	0.06	0.207	6.34	0.04	0.30	1dosA	GO:0003824 GO:0004332 GO:0005618 GO:0005829 GO:0005886 GO:0005975 GO:0006094 GO:0006096 GO:0008270 GO:0016829 GO:0016832 GO:0046872
14	0.06	0.190	6.08	0.06	0.27	4gquA	GO:0000105 GO:0004424 GO:0005737 GO:0008652 GO:0016829 GO:0040007
15	0.06	0.199	5.42	0.05	0.26	4pr3A	GO:0003824 GO:0008152 GO:0008782 GO:0008930 GO:0009116 GO:0016787 GO:0016798
16	0.06	0.180	4.87	0.05	0.23	2gd9A	GO:0000455 GO:0005829 GO:0008703 GO:0009231 GO:0009982 GO:0016020 GO:0016021 GO:0019239 GO:0055114
17	0.06	0.200	6.84	0.04	0.30	1zcwA	GO:0016740
18	0.06	0.163	4.61	0.04	0.20	1ns5B	GO:0005737 GO:0006364 GO:0008168 GO:0016740 GO:0031167 GO:0032259 GO:0042803 GO:0043022 GO:0070038 GO:0070475

Consensus prediction of GO terms

Molecular Function	GO:0008239	GO:0047658
GO-Score	0.65	0.32
Biological Processes	GO:0006508	GO:0019439	GO:0042737	GO:0050783	GO:1901361	GO:0046700	GO:0009822
GO-Score	0.65	0.46	0.46	0.46	0.46	0.46	0.46
Cellular Component	GO:0044424
GO-Score	0.46

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.