I-TASSER results

I-TASSER results for job id Rv1204c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (562 residues)
MRVWKHVEAAVDSPDRCGVVLVGPHGVGKTLLAQLAAEQVMSEDGRSGRARWVVGTAPGR
AIPFGAFRHLISLPASGADIGRPAALLRAARSSLTGDAGDLLLVVDDAHNLDPLSATLVY
QLARAGAARLVVTVASEAEPPDAIAALWSDDLLTRVAIEPLDRAQTAAFVESALDATLDV
ADADELFRRSLGNPLYLRHLIDGGGLEHVDGRWRCRDEDRRPLSGVIDEYLCALPEPARA
VVDYLAIAEPLARTDLVALVGGEQLDTLGQAEAAGAVRVGPDSDTSEIFVGHPLYADRAR
AVLTAEHAHALRVSLVAQLAKHPSDHVSDQLRLSSLAIDVPASATPAAVTDAATAAGQAL
RLGDVRLAERLARAALDRSDALAARLPLAYALGWQGRGREADAVLAAVNPAELTETELMA
WAIPRAANRFWMLNEPERATAFLQTTRSRVTEPTARSTLDALAATFAMNSGNLPRAITLA
TEVLSGPAADDMAVAWAASAAALSSARMGRFGDVDRLAERASAAEHPGLLRFTVGLAQIT
SLLLAGDVAPAQELAKRFTDFA

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MRVWKHVEAAVDSPDRCGVVLVGPHGVGKTLLAQLAAEQVMSEDGRSGRARWVVGTAPGRAIPFGAFRHLISLPASGADIGRPAALLRAARSSLTGDAGDLLLVVDDAHNLDPLSATLVYQLARAGAARLVVTVASEAEPPDAIAALWSDDLLTRVAIEPLDRAQTAAFVESALDATLDVADADELFRRSLGNPLYLRHLIDGGGLEHVDGRWRCRDEDRRPLSGVIDEYLCALPEPARAVVDYLAIAEPLARTDLVALVGGEQLDTLGQAEAAGAVRVGPDSDTSEIFVGHPLYADRARAVLTAEHAHALRVSLVAQLAKHPSDHVSDQLRLSSLAIDVPASATPAAVTDAATAAGQALRLGDVRLAERLARAALDRSDALAARLPLAYALGWQGRGREADAVLAAVNPAELTETELMAWAIPRAANRFWMLNEPERATAFLQTTRSRVTEPTARSTLDALAATFAMNSGNLPRAITLATEVLSGPAADDMAVAWAASAAALSSARMGRFGDVDRLAERASAAEHPGLLRFTVGLAQITSLLLAGDVAPAQELAKRFTDFA
Prediction	HHHHHHHHHHHHCCCCCEEEEEHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHCCCCCCHHHHHHHHHHHHHCCCCEEEEHCCCCCCCHHHHHHHHCCCCEEEHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCEEEEHCCCCCEEEEHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
Conf.Score	7899999999985898599999589998999999999999987786579999976777667865788887657778888775689999999999857998899995876578899999999998589868999869998765678877568866998799999999999999979999999999999999998999999999777555677666678887569999999999799999999999998698889999998678878999999998885776568887899856899999998599999999999999999985888757899999999756877558999999999999998798999999999876678888999999999999899999999999866578886889999999999999997999999999999998589976899999999999999699999999999999757788678999999999999987998999999999999766676568999999999999899999999999999979
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 500 520 540 560 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MRVWKHVEAAVDSPDRCGVVLVGPHGVGKTLLAQLAAEQVMSEDGRSGRARWVVGTAPGRAIPFGAFRHLISLPASGADIGRPAALLRAARSSLTGDAGDLLLVVDDAHNLDPLSATLVYQLARAGAARLVVTVASEAEPPDAIAALWSDDLLTRVAIEPLDRAQTAAFVESALDATLDVADADELFRRSLGNPLYLRHLIDGGGLEHVDGRWRCRDEDRRPLSGVIDEYLCALPEPARAVVDYLAIAEPLARTDLVALVGGEQLDTLGQAEAAGAVRVGPDSDTSEIFVGHPLYADRARAVLTAEHAHALRVSLVAQLAKHPSDHVSDQLRLSSLAIDVPASATPAAVTDAATAAGQALRLGDVRLAERLARAALDRSDALAARLPLAYALGWQGRGREADAVLAAVNPAELTETELMAWAIPRAANRFWMLNEPERATAFLQTTRSRVTEPTARSTLDALAATFAMNSGNLPRAITLATEVLSGPAADDMAVAWAASAAALSSARMGRFGDVDRLAERASAAEHPGLLRFTVGLAQITSLLLAGDVAPAQELAKRFTDFA
Prediction	5430430141023240201010013100110000000310251311000000000021012000000030041145444232112002101310233412010042422101200020021002123010000000334123102200443403203043243610020024105350234003100210220000000012102143342113042321210130022006403430220020000011001200110045321500230032101211334442100000000010022103351011001000100141325212101000100130444212300310130021012220031011001101435421300010010011131142024004403634243210000000000100111111320130043026106533110000000000000120032014004401524521310000000000000011011420130042013103012100000000000000010042014004201720
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480                 500                 520                 540                 560

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

HHHHHHHHHHHHCCCCCEEEEEHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHCCCCCCHHHHHHHHHHHHHCCCCEEEEHCCCCCCCHHHHHHHHCCCCEEEHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHCCEEEEHCCCCCEEEEHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHC
MRVWKHVEAAVDSPDRCGVVLVGPHGVGKTLLAQLAAEQVMSEDGRSGRARWVVGTAPGRAIPFGAFRHLISLPASGADIGRPAALLRAARSSLTGDAGDLLLVVDDAHNLDPLSATLVYQLARAGAARLVVTVASEAEPPDAIAALWSDDLLTRVAIEPLDRAQTAAFVESALDATLDVADADELFRRSLGNPLYLRHLIDGGGLEHVDGRWRCRDEDRRPLSGVIDEYLCALPEPARAVVDYLAIAEPLARTDLVALVGGEQLDTLGQAEAAGAVRVGPDSDTSEIFVGHPLYADRARAVLTAEHAHALRVSLVAQLAKHPSDHVSDQLRLSSLAIDVPASATPAAVTDAATAAGQALRLGDVRLAERLARAALDRSDALAARLPLAYALGWQGRGREADAVLAAVNPAELTETELMAWAIPRAANRFWMLNEPERATAFLQTTRSRVTEPTARSTLDALAATFAMNSGNLPRAITLATEVLSGPAADDMAVAWAASAAALSSARMGRFGDVDRLAERASAAEHPGLLRFTVGLAQITSLLLAGDVAPAQELAKRFTDFA

4ui9O

0.10

0.09

0.93

1.07

MUSTER

RRRLNQQLLPLLQGPDIT----------VSKLLKLIEESCPQLANSVQIRIKLMAEGELKDM-FDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLS-------------SQVFKLYTALQQYFQSD-----ENKKPLSQKQAEFFLSQQASLLKNDELTPASLQKELNNLLKFNPDAHYLSYLNNLRVQDFSSTHSLLHYFTGAESKSNGEEGYGRSLRYAALNLAALHC-HVCLQHCLSWLYVLGQKRLEHSVKKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHW-LSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEQQNESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPLCDQKIQFDRAMNDGKYHLADSLVTGITALNS------IEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPIGAILDKGRAMFLVAKCQVASAALEAAIENLNEAKNYF

4ui9O

0.10

0.09

0.93

1.04

dPPAS

RRRLNQQLLPLLQGPDIT--------------VSKLLKLIEESCPQLANSVQIRIKLMAEGELFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSF------------SQVFKLYTALQQYFQSD-----ENKKPLSQKQAEFFLSQQASLLKNDELTPASLQKELNNLLKFNPDAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRSDSYVLLE---HSVKKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLAVALCHLAELHAEQGCFAAASEVLKHLKERSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITA-----LNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPI

2qenA

0.13

0.07

0.52

1.21

wdPPAS

SRKLEESLEN------PLTLLLGIRRVGKSSLLRAFLNER---PGILIDCRELYAERGHITRPFQKFQSKFKISLNLEPRKLSLREVFRELNDLGEELGEFIVAFDEAQYLGKELLALFAYAYDSLPNLKIILTGSEVGLLHDFLKITDYESAGEVLVKPFDKDTSVEFLKRGF-LDVPENEIEEAVELLDGIPGWLVVFGVEYLRNGDFGRAKRTLEVAKGLIMGELEELRRRSPRYVDILRAIAL-GYNRWSLIRDYLAKIPEPRLYALLENLKKMNWIVEEDNTYKIADPVVATVLRI---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

4ui9O

0.10

0.09

0.93

1.23

wMUSTER

RRRLNQQLLPLLQGPDI-----------VSKLLKLIEESCPQLANSVQIRIKLMAEGELKDM-FDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSK-------------LSSQVFKLYTALQQYFQSD-----ENKKPLSQKQAEFFLSQQASLLKNDELTPASLQKELNNLLKFNPDAHYLSYLNNLRVQDFSSTHSLLHYFTGAESKSNGEEGYGRSLRYAALNLAALHC--LALQEAIRIAQESNDQHCLSWLYVLGQKSYVLLEHSVKKALVQQRAFAGKTANKLMDALKDSDLLHW-LSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEQQNESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPLCDQKIQFDRAMNDGKYHLADSLVTGITALNS------IEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPIGAILDKGRAMFLVAKCQVASA-LEAAIENLNEAKNYF

2qenA

0.14

0.07

0.51

1.32

wPPAS

SRKLEESLEN------PLTLLLGIRRVGKSSLLRAFLNER---PGILIDCRELYAERGHITRPFQKFQSKFKISLNLEPRKLSLREVFRELNDLGEELGEFIVAFDEAQYLGKELLALFAYAYDSLPNLKIILTGSEVGLLHDFLKITDYESAGEVLVKPFDKDTSVEFLKRGF--DVPENEIEEAVELLDGIPGWLVVFGVEYLRNGDFGRA--TLEVAKGLIMGELEELRRRSPRYVDILRAIAL-GYNRWSLIRDYLAKIPEPRLYALLENLKKMNWIVEEDNTYKIADPVVATVLRI---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

4ui9O

0.11

0.10

0.90

1.57

dPPAS2

VSKLLKLIEESC-----------------PQLANSVQIRIKLMAEGELKDMEQF---------FDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSF------------SQVFKLYTALQQYFQSD-----ENKKPLSQKQAEFFLSQQASLLKNDELTPASLQKELNNLLKFNPDAHYLSYLNNLRVQDFSSTHSLLHYFLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELA-----LQEAIRIAQESNDHVCLQHCLSWLYVLGQKRSDSYVLLE-HSVKKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLAVALCHLAELHAEQGCFAAASEVLKHLKERSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITA-----LNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPI

4ui9O

0.13

0.12

0.95

1.34

PPAS

MEQFFDDLSDSFSGTEPEVHKTSVVGLQVFKLYTALQQYFQSDE---------NKKPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFLSYLNNLRVQDFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAA------LNLAALALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRSDSYVLLEHSVKKAVHFGLPYLA---SLGIQSLVQQRAFAGKT-ANKLMDALKD-----SDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEQQNNTESFAVALCHLAELHAEQGC--SEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITAL-NSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPADGAILDKGRAMFLVAKCQVASAALEAAIENLNEAKNY-DCKERIRDVVYFQARLYHTLGKTQERNRCAMLFRQLH

2a5yB2

0.15

0.09

0.58

1.23

Env-PPAS

VDRVIKKLDEMCDLDSFFLFLHGRAGSGKSVIASQALSKSDLIGINYDSIVWLKDSGTAPKSTFDLFTDILLLLNFPSVEHVTSVVLKRMICNALIDRPNTLFVFDDV-V-QEETIRWAQELR----LRCLVTTRD-----VEISNAA-SQTCEFIEVTSLEIDECYDFLEAYGMPMPEEDVLNKTIELSSGNPATLMMFFKSCEPRGLVGVECITPYSYKSLAMALQRCVEVLSDEDRSALAFAVVMGVDIPVKLWSCVIPVEADRLKRLSKRGALLSGKRMPVLTFKI-DHIIHMFLKHVVDAQTIANGISILEQRLLEIRPEDFPKFMQLHQKFYDSL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

1b3uA

0.10

0.09

0.91

1.00

MUSTER

LYPIAVLIDELRNEDVQL----------RLNSIKKLSTIALALGVERTRLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVV----------DKAVESLRAISHEHSPSDL-----EAHFVPLVKRLAGGDWFTS-------RTSACGLFSVCYPR-VSSAVKAELRQYFR-TPMVRRAAASKLGE--------AKVLELDNVKSEIIPMFSNL-LAVEACVNIAQLLPQLVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPE----ITKTDLVPAFQNLMEAEVRAAASHKVKEFCENLSADCSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECRLNIISNLDCVNEVIGIRQLSQSLLPAIVELADAKWRVRLAIIEYMPLLAGQLGFDEKLNSLCMAWLVD-HVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLT

1hz4A

0.13

0.07

0.54

0.88

dPPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EIKDIREDTMHAEFNALRAQVAINDGNPDEAERLAKLA-----LEELPPG-----------------WFYSRIVATSVLGEVLHCKGELTRSLALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLPMHEFLVRIRAQLLWAWARLDEAEASARSGIEVSSYQPQQQLQCLAMLIQCSLARGDLDNARSQLNRLENLKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFANNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLLNRNLLLLNQLYWQAGRKSDAQRVLLDALKLA

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 2 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-1.29

Estimated TM-score = 0.55±0.15

Estimated RMSD = 10.6±4.6Å

Download Model 2

C-score=-1.72

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4ui9O	0.852	2.82	0.095	0.936
2	5c6gA	0.591	5.07	0.064	0.754
3	4ui9X	0.574	4.93	0.105	0.728
4	4xdnA	0.573	5.17	0.067	0.731
5	2xpiA	0.562	5.07	0.076	0.722
6	4uzyA	0.561	4.65	0.097	0.705
7	5dseA	0.500	5.61	0.052	0.680
8	4ui9J	0.494	5.66	0.075	0.667
9	4ui9C	0.479	5.80	0.059	0.651
10	4rg6A	0.475	5.40	0.069	0.621

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.09	4	2hcbC	MG	Rep, Mult	29,30,106,107
2	0.07	3	1b86A	OXY	Rep, Mult	309,313
3	0.05	2	4gpkJ	III	Rep, Mult	354,356,417,420,421,424,428,458,461,462,496,499,500,530,533,534,537
4	0.05	2	2bo6B	MN	Rep, Mult	106,107
5	0.05	2	2hcbA	MG	Rep, Mult	30,106,107
6	0.02	1	2qfcA	III	Rep, Mult	233,236,239,240,269,272,273,276,306,309,312,350
7	0.02	1	2qbyA	MG	Rep, Mult	30,106,195
8	0.02	1	4f8hA	LMD	Rep, Mult	353,356
9	0.02	1	5lcwO	III	Rep, Mult	153,157,182,185,186,204,210,229,233,240,269,273,276,305,309,312,313,316,320,346,350,354,357,386,387,417,492
10	0.02	1	2qbyB	MG	Rep, Mult	30,106,136
11	0.02	1	5irzD	6OE	Rep, Mult	115,119
12	0.02	1	3cmvH	ANP	Rep, Mult	310,311,312,313,314,315,316,329,358
13	0.02	1	3h45O	PO4	Rep, Mult	314,317
14	0.02	1	4i9cA	III	Rep, Mult	272,309,313,316,320,350,353,354,357,386,417,420,454,455,458

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	2dqmA	0.345	7.17	0.055	0.532	3.4.11.2	NA
2	0.060	3h09B	0.351	7.75	0.040	0.571	3.4.21.72	NA
3	0.060	3ffzA	0.346	7.86	0.019	0.575	3.4.24.69	NA
4	0.060	2eabA	0.384	6.85	0.039	0.582	3.2.1.63	NA
5	0.060	3ecqB	0.341	8.13	0.057	0.584	3.2.1.97	NA
6	0.060	1ej6A	0.340	7.77	0.046	0.560	2.7.7.50	331
7	0.060	2vuaA	0.237	7.33	0.041	0.372	3.4.24.69	201
8	0.060	2jgdA	0.348	8.06	0.056	0.585	1.2.4.2	415
9	0.060	1dl2A	0.342	5.74	0.038	0.470	3.2.1.113	NA
10	0.060	1llwA	0.364	7.81	0.063	0.598	1.4.7.1	NA
11	0.060	2gq3A	0.343	7.79	0.055	0.578	2.3.3.9	NA
12	0.060	1w6jA	0.374	6.47	0.058	0.536	5.4.99.7	NA
13	0.060	2vdcA	0.343	8.01	0.049	0.573	1.4.1.13	NA
14	0.060	2pmzQ	0.327	6.90	0.053	0.498	2.7.7.6	NA
15	0.060	2gtqA	0.353	7.34	0.048	0.557	3.4.11.2	NA
16	0.060	2zxqA	0.340	8.24	0.051	0.587	3.2.1.97	181
17	0.060	2cqsA	0.378	7.42	0.063	0.596	2.4.1.20	417
18	0.060	2vdcF	0.363	7.82	0.051	0.601	1.4.1.13	18,30
19	0.060	2w00B	0.347	7.39	0.054	0.541	3.1.21.3	239

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.26	0.838	2.96	0.09	0.93	5g05O	GO:0005634 GO:0005654 GO:0005680 GO:0005829 GO:0007049 GO:0007067 GO:0016567 GO:0019903 GO:0031145 GO:0042787 GO:0043161 GO:0051301 GO:0051436 GO:0051437 GO:0051439 GO:0070979
1	0.09	0.573	5.17	0.07	0.73	4xdnA	GO:0000790 GO:0005634 GO:0006302 GO:0007049 GO:0007059 GO:0007064 GO:0007067 GO:0007076 GO:0032116 GO:0051301 GO:0070550 GO:0071169
2	0.07	0.591	5.07	0.06	0.75	5c6gA	GO:0000790 GO:0006302 GO:0007064 GO:0007076 GO:0032116 GO:0070550 GO:0071169
3	0.07	0.564	5.46	0.08	0.75	5a31Y	GO:0005634 GO:0005654 GO:0005680 GO:0005829 GO:0007049 GO:0007067 GO:0016567 GO:0019903 GO:0031145 GO:0042787 GO:0043161 GO:0051301 GO:0051436 GO:0051437 GO:0051439 GO:0070979
4	0.07	0.506	5.87	0.08	0.71	5a31F	GO:0005634 GO:0005654 GO:0005680 GO:0005737 GO:0005813 GO:0005819 GO:0005829 GO:0005876 GO:0007091 GO:0008283 GO:0016567 GO:0019903 GO:0031145 GO:0042787 GO:0043161 GO:0051436 GO:0051437 GO:0051439 GO:0070979
5	0.07	0.479	5.80	0.06	0.65	5a31C	GO:0004842 GO:0005622 GO:0005654 GO:0005680 GO:0005829 GO:0006511 GO:0007049 GO:0007067 GO:0007080 GO:0007091 GO:0007096 GO:0016567 GO:0030071 GO:0031145 GO:0042787 GO:0043161 GO:0051301 GO:0051436 GO:0051437 GO:0051439 GO:0070979
6	0.06	0.394	5.60	0.08	0.53	1w3bB	GO:0000123 GO:0000791 GO:0001933 GO:0001934 GO:0005547 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006041 GO:0006110 GO:0006486 GO:0006493 GO:0006915 GO:0007165 GO:0007584 GO:0008047 GO:0008134 GO:0008289 GO:0008375 GO:0010628 GO:0010801 GO:0016020 GO:0016262 GO:0016568 GO:0016740 GO:0016757 GO:0019904 GO:0030854 GO:0030900 GO:0031397 GO:0032868 GO:0032869 GO:0032922 GO:0033137 GO:0035020 GO:0042277 GO:0042588 GO:0043005 GO:0043025 GO:0043085 GO:0043981 GO:0043982 GO:0043984 GO:0043995 GO:0043996 GO:0045793 GO:0045862 GO:0045944 GO:0046626 GO:0046972 GO:0048015 GO:0048029 GO:0048312 GO:0048511 GO:0051571 GO:0060548 GO:0061087 GO:0070207 GO:0070208 GO:0070688 GO:0071222 GO:0071300 GO:0071333 GO:0080182 GO:0090315 GO:0090526 GO:0097237 GO:0097363 GO:1900038 GO:1900182 GO:1903428
7	0.06	0.405	5.26	0.08	0.53	1w3bA	GO:0000123 GO:0000791 GO:0001933 GO:0001934 GO:0005547 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006041 GO:0006110 GO:0006486 GO:0006493 GO:0006915 GO:0007165 GO:0007584 GO:0008047 GO:0008134 GO:0008289 GO:0008375 GO:0010628 GO:0010801 GO:0016020 GO:0016262 GO:0016568 GO:0016740 GO:0016757 GO:0019904 GO:0030854 GO:0030900 GO:0031397 GO:0032868 GO:0032869 GO:0032922 GO:0033137 GO:0035020 GO:0042277 GO:0042588 GO:0043005 GO:0043025 GO:0043085 GO:0043981 GO:0043982 GO:0043984 GO:0043995 GO:0043996 GO:0045793 GO:0045862 GO:0045944 GO:0046626 GO:0046972 GO:0048015 GO:0048029 GO:0048312 GO:0048511 GO:0051571 GO:0060548 GO:0061087 GO:0070207 GO:0070208 GO:0070688 GO:0071222 GO:0071300 GO:0071333 GO:0080182 GO:0090315 GO:0090526 GO:0097237 GO:0097363 GO:1900038 GO:1900182 GO:1903428
8	0.06	0.377	6.62	0.06	0.55	4i2wA	GO:0005886 GO:0005938 GO:0009792 GO:0018991 GO:0031625 GO:0032154 GO:0040011 GO:0042802 GO:0045214
9	0.06	0.388	6.11	0.05	0.53	4xifA	GO:0000123 GO:0000791 GO:0001933 GO:0001934 GO:0005547 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006041 GO:0006110 GO:0006486 GO:0006493 GO:0006915 GO:0007165 GO:0007584 GO:0008047 GO:0008134 GO:0008289 GO:0008375 GO:0010628 GO:0010801 GO:0016020 GO:0016262 GO:0016568 GO:0016740 GO:0016757 GO:0019904 GO:0030854 GO:0030900 GO:0031397 GO:0032868 GO:0032869 GO:0032922 GO:0033137 GO:0035020 GO:0042277 GO:0042588 GO:0043005 GO:0043025 GO:0043085 GO:0043981 GO:0043982 GO:0043984 GO:0043995 GO:0043996 GO:0045793 GO:0045862 GO:0045944 GO:0046626 GO:0046972 GO:0048015 GO:0048029 GO:0048312 GO:0048511 GO:0051571 GO:0060548 GO:0061087 GO:0070207 GO:0070208 GO:0070688 GO:0071222 GO:0071300 GO:0071333 GO:0080182 GO:0090315 GO:0090526 GO:0097237 GO:0097363 GO:1900038 GO:1900182 GO:1903428
10	0.06	0.305	6.00	0.08	0.43	3cv0A	GO:0005052 GO:0005778 GO:0005829 GO:0016560
11	0.06	0.383	6.17	0.06	0.53	5a01A	GO:0006486 GO:0006493 GO:0008375 GO:0016740 GO:0016757 GO:0035220 GO:0045475
12	0.06	0.281	5.09	0.06	0.36	2y4uA	GO:0004860 GO:0005737 GO:0005783 GO:0005788 GO:0005790 GO:0005829 GO:0006417 GO:0006986 GO:0016020 GO:0019901 GO:0031205 GO:0036494 GO:0036498 GO:0043066 GO:0051087 GO:0051603 GO:0051607 GO:0051787 GO:0070062 GO:0070417 GO:1903561 GO:1903912 GO:1904030
13	0.06	0.281	3.54	0.09	0.33	2pl2A
14	0.06	0.208	8.19	0.05	0.35	1xl7B	GO:0005102 GO:0005739 GO:0005777 GO:0006091 GO:0006629 GO:0006631 GO:0006635 GO:0006810 GO:0008458 GO:0009437 GO:0010243 GO:0015908 GO:0015936 GO:0016740 GO:0016746 GO:0042493 GO:0043231 GO:0051791
15	0.06	0.244	4.90	0.08	0.31	4houA	GO:0002376 GO:0003723 GO:0005737 GO:0005829 GO:0009615 GO:0016032 GO:0019060 GO:0032091 GO:0043657 GO:0045070 GO:0045071 GO:0045087 GO:0050688 GO:0050689 GO:0051097 GO:0051607 GO:0060337 GO:0071357 GO:0071360
16	0.06	0.230	4.48	0.09	0.28	2q7fA
17	0.06	0.231	4.03	0.13	0.27	2e2eA
18	0.06	0.211	6.71	0.06	0.32	3vylA	GO:0046872

Consensus prediction of GO terms

Molecular Function	GO:0019903
GO-Score	0.36
Biological Processes	GO:0051301	GO:0070979	GO:0031145	GO:0051437	GO:0051436	GO:0000070	GO:0071168	GO:0007062	GO:0030261	GO:0070199	GO:0006281
GO-Score	0.37	0.36	0.36	0.36	0.36	0.30	0.30	0.30	0.30	0.30	0.30
Cellular Component	GO:0005654	GO:0005829	GO:0005680	GO:0044454	GO:0000785
GO-Score	0.36	0.36	0.36	0.30	0.30

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.