I-TASSER results

I-TASSER results for job id Rv1129c

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (486 residues)
MTRSNVLPVARTYSRTFSGARLRRLRQERGLTQVALAKALDLSTSYVNQLENDQRPITVP
VLLLLTERFDLSAQYFSSDSDARLVADLSDVFTDIGVEHAVSGAQIEEFVARMPEVGHSL
VAVHRRLRAATEELEGYRSRATAETELPPARPMPFEEVRDFFYDRNNYIHDLDMAAERMF
TESGMRTGGLDIQLAELMRDRFGISVVIDDNLPDTAKRRYHPDTKVLRVAHWLMPGQRAF
QIATQLALVGQSDLISSIVATDDQLSTEARGVARIGLANYFAGAFLLPYREFHRAAEQLR
YDIDLLGRRFGVGFETVCHRLSTLQRPRQRGIPFIFVRTDKAGNISKRQSATAFHFSRVG
GSCPLWVVHDAFAQPERIVRQVAQMPDGRSYFWVAKTTAADGLGYLGPHKNFAVGLGCDL
AHAHKLVYSTGVVLDDPSTEVPIGAGCKICNRTSCAQRAFPYLGGRVAVDENAGSSLPYS
STEQSV

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTRSNVLPVARTYSRTFSGARLRRLRQERGLTQVALAKALDLSTSYVNQLENDQRPITVPVLLLLTERFDLSAQYFSSDSDARLVADLSDVFTDIGVEHAVSGAQIEEFVARMPEVGHSLVAVHRRLRAATEELEGYRSRATAETELPPARPMPFEEVRDFFYDRNNYIHDLDMAAERMFTESGMRTGGLDIQLAELMRDRFGISVVIDDNLPDTAKRRYHPDTKVLRVAHWLMPGQRAFQIATQLALVGQSDLISSIVATDDQLSTEARGVARIGLANYFAGAFLLPYREFHRAAEQLRYDIDLLGRRFGVGFETVCHRLSTLQRPRQRGIPFIFVRTDKAGNISKRQSATAFHFSRVGGSCPLWVVHDAFAQPERIVRQVAQMPDGRSYFWVAKTTAADGLGYLGPHKNFAVGLGCDLAHAHKLVYSTGVVLDDPSTEVPIGAGCKICNRTSCAQRAFPYLGGRVAVDENAGSSLPYSSTEQSV
Prediction	CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEHCCCCCCCCEEEEHCCCCEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEEHCCCCCEEEEHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEHCCCCEEEEEEEEHCCCCCCCCCCCCEEEEEHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
Conf.Score	986557777654566668899999999999999999999899999999999799999999999999998999889758886567789999987555566888899999999775789999999999999999999999876567777765568699999999998765799999999999998898887689999999999797999857888876567767998899779998554578999999999987899999975789998999999999999999998775999999999989999999999899999999999979998766975899998788977888658898888888888765899998779958999999599976999999867888887888865799976668887765455678888888887668875555788885557888889876788766666777788899
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460 480 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MTRSNVLPVARTYSRTFSGARLRRLRQERGLTQVALAKALDLSTSYVNQLENDQRPITVPVLLLLTERFDLSAQYFSSDSDARLVADLSDVFTDIGVEHAVSGAQIEEFVARMPEVGHSLVAVHRRLRAATEELEGYRSRATAETELPPARPMPFEEVRDFFYDRNNYIHDLDMAAERMFTESGMRTGGLDIQLAELMRDRFGISVVIDDNLPDTAKRRYHPDTKVLRVAHWLMPGQRAFQIATQLALVGQSDLISSIVATDDQLSTEARGVARIGLANYFAGAFLLPYREFHRAAEQLRYDIDLLGRRFGVGFETVCHRLSTLQRPRQRGIPFIFVRTDKAGNISKRQSATAFHFSRVGGSCPLWVVHDAFAQPERIVRQVAQMPDGRSYFWVAKTTAADGLGYLGPHKNFAVGLGCDLAHAHKLVYSTGVVLDDPSTEVPIGAGCKICNRTSCAQRAFPYLGGRVAVDENAGSSLPYSSTEQSV
Prediction	565553352464331210041014005626010430073160034002301345330316002200510624042025444542242023004324252424331034004301520200030031034024304402431454342252132002101200220400032004001300530514432013201410263140101224512521112112621102015304443100200200011212410342055361436303300330002000000000153015105624200310162150000000000110331622401000000131010122242630201322100010000200222520110101024131000000002334432543422000000000420430010342427655433201110220317604300112163604035422111103135667
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400                 420                 440                 460                 480

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEHCCCCCCCCEEEEHCCCCEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEEHCCCCCEEEEHCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEHCCCCEEEEEEEEHCCCCCCCCCCCCEEEEEHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
MTRSNVLPVARTYSRTFSGARLRRLRQERGLTQVALAKALDLSTSYVNQLENDQRPITVPVLLLLTERFDLSAQYFSSDSDARLVADLSDVFTDIGVEHAVSGAQIEEFVARMPEVGHSLVAVHRRLRAATEELEGYRSRATAETELPPARPMPFEEVRDFFYDRNNYIHDLDMAAERMFTESGMRTGGLDIQLAELMRDRFGISVVIDDNLPDTAKRRYHPDTKVLRVAHWLMPGQRAFQIATQLALVGQSDLISSIVATDDQLSTEARGVARIGLANYFAGAFLLPYREFHRAAEQLRYDIDLLGRRFGVGFETVCHRLSTLQRPRQRGIPFIFVRTDKAGNISKRQSATAFHFSRVGGSCPLWVVHDAFAQPERIVRQVAQMPDGRSYFWVAKTTAADGLGYLGPHKNFAVGLGCDLAHAHKLVYSTGVVLDDPSTEVPIGAGCKICNRTSCAQRAFPYLGGRVAVDENAGSSLPYSSTEQSV

1b0nA

0.20

0.04

0.21

1.63

dPPAS2

----------------MIGQRIKQYRKEKGYSLSELAEKAGVAKSYLSSIERNLQTPSIQFLEKVSAVLDVSVHTLLDEKHETLDSEWEKLVRDAMT-SGVSKKQFREFLDYQKWRKSQ-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

3dteA

0.15

0.07

0.47

1.38

wdPPAS

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------PTALAAAKARRELAA-----GAGLPGRDTHSLHGLDGITLT-FPGQRDGAYDPEHHVILINSQVRPERQRFTLAHEISHALLLGDDDLLSDLHDEYEGDRLEQVIETLCNVGAAALL-PAELIDDLLTRFGPTLAELARRADVSATSALYALAERTAPPVIYAVCALSRAKE--TVRASSASAGVKYSLSAGT----PAALALDT-RLPLAQDSYVPFGRRPAYVDAFPERQ---------RVLVSFALP-------------------------------------------------------------------

3vk0A

0.18

0.03

0.18

1.30

Env-PPAS

LTLPAELPDEQD-LRAVLAYNMRLFRVNKGWSQEELARQCGLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLL-PPQERLKLMTN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

3dteA

0.16

0.07

0.47

1.29

wPPAS

-------------------------------------------------------------------------------------------------------------------------------------------------------------------------PTALAAAKARRELAASYGAGLP------THSLHGLDGITLT-FPGQRDGAYDPEHHVILINSQVRPERQRFTLAHEISHALLLGDDDLLSDLHDEYEGDRLEQVIETLCNVGAAALL-PAELIDDLLTRFGPTLAELARRADVSATSALYALAERTAPPVIYAVCALSRAKE--TVRASSASAGVKYSLSAGT-PD-PAALALDT-RLPLAQDSYVPFGRRPAYVDAFPER---------QRVLVSFALP-------------------------------------------------------------------

3f51A

0.19

0.04

0.19

1.59

dPPAS2

---------PEPLLREALGAALRSFRADKGVTLRELAEASRVSPGYLSELERGRKEVSSELLASVCHALGASVADVLIEAAGSMALQAAQEDLARVLEWSHPQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

3we0A

0.10

0.97

0.74

MUSTER

LEHPAGLGSIPAARHGIAGLVAAYELMKLGLKPVVYASKMGLRSQAFNIAELGGMRVSSTAFYHYVDKLGLETKPFPNPLTPASRSTV--IDLEGQTYYAEKAADLPALFQEVTDAWADALESGARFGDIQQAIRDRDVPRLKELWNTLVPLWDDRTFYDFVATSK-AFAKLSFQHREVFGQVGFGTGGWDSDFPNSMLEIF----RVVMTNCDDHQHLVVGGVEQVGIWRHVPERCAHWPEGTSLSSLHGGAPRTGVKRIARASDGRLAVTDNWGDCRHYAAVLTTCQSWLLTTCEESLFSQKMWMALDRTRYMQSSRPFWKDKDPETGRDLMSMTLTDRLTRGTYLFDNGD---DKPGVICLSYAWHPVEKRVQLALDALKKIPKTDIAHIIGDPITISWEADPHFLGAFKGALPGHYRYNQRM-YAHFMQAQMPVEQRGI-AGDDVSWTPAWSLNAVWGIMNHFGTHADNPGIGQIALAD---

2qfcA

0.12

0.06

0.55

0.78

dPPAS

--------------AEKLGSEIKKIRVLRGLTQKQLSENI-CHQSEVSRIESGAVYPSMDILQGIAAKLQIPIIHFYESDIERKKQFKDQVIMLCKQKRYKEIYNKVWNELKKEWQYYVAAYVLKKVDYEYCILELKKLLNQQLTGIDVYQNLYIENAIANIYAENGYLKKGIDLFEQILKQLEALHDNEEFDVKVRYNHAK---ALYLDSRYEESLYQVNK---AIEISCRINSMALIGQLYYQRGECLRKLEYEEAEIEDAY--KKASFFFDILEMHAYKEALVNKISRL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

4ui9I

0.11

0.10

0.90

0.83

wMUSTER

LETNLLYSFLPVTRMARKFTHISALLQYINLSLTCMCEAW---DSRLTKFVQEKNTTTQDEFMHLLL--KASQTLLMNQLTVKGLKKLGQSIESS-------YSSIQKLVIS--HLQSGSESLLYHLSELKGMASW-KQKYEPLGLDAAGIEEAITAVGSFILKANELLQVIDSSMKNFKAFFRKMTQKDITFVAEFLTEHFNFNV--------ERVGQYLKDEDDDLVSPPNTEGNQWYDFLQNSSHLKESPLL------FPYYPRKSLHFVKRRMENIIDQCLQKPADVIGKSMNQATRSEDSTRRLFKFPF---------LWNNKTSNLHYLLFTILEDSLILRRHTDISQSVSNG-GSFTY-ATTEKVRRSIYSCLDAQFYDDE--TVTVVLKDTVGREG----RDRLLVQL--PLSLVYNSEDSAEYQFTGTYSTRLDAIPTRTMHFEK-HWRLLESMFRKVSLSSNLRHVRVFEMDIDDE

2qfcA

0.12

0.07

0.55

1.04

PPAS

--------------AEKLGSEIKKIRVLRGLTQKQLSENI-CHQSEVSRIESGAVYPSMDILQGIAAKLQIPIIHFYEVLIYSDIERKKQFKDQIMLCKQKRYKEIYNKVWNELKK-----EEYHPEFQQFLQWQYYVAAYVLKKVDYEYCILELKKLLNQQLTGIDVYQNLYIENAIANIYAENGYLKKGIDLFEQILKQL---------------------------EALHDNEEFDVKVRYNHAKALYLDSRYEESLY-----NKAIEISCRINSMALIGQLYYQRGECLRKLE---YEEAEIEDAYKKAKEALVNKISRL------------------------------------------------------------------------------------------------------------------------------------------------------------------

4um2A

0.10

0.09

0.89

0.72

MUSTER

---------------SMELHRLLRVADNQELQLSNLLSRDRISPEGLEKMAQ----LRAELLQLYERCILLDIEFSDNQN-KNAFYQVIEKFRQLVKDPNVE--NPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMD-GLAIRLRKTVKYALISAQRCMICQGDIARYREQASDTANYGKARSWYLKAQHIAPKNGRLLAVYTRRKLDAVYYY---MRSLAASNP-------ILTAKESLMSLFEETKRKAEQMEKGLGSLSPSDLNKRFILSFLHAHGKGMETFPAVAEKVLKEFQVLLQHSPSPISTRMLQLMTI----------NMFAVHNSQRSVIQEQAAALGLAMF------SLLVRRCTCLLKESLSSPEDQDDQDD----IKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPSHVAVDVWSTLTAVNQSEVPLYKDPDDDLTLLIDRLLSGFVPLLAAPQDP

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-3.60

Estimated TM-score = 0.32±0.11

Estimated RMSD = 16.4±3.0Å

Download Model 2

C-score=-3.89

Download Model 3

C-score=-4.38

Download Model 4

C-score=-4.88

Download Model 5

C-score=-4.88

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	4um2A	0.801	2.88	0.085	0.883
2	1ya0B	0.662	3.84	0.071	0.782
3	3zheB	0.568	4.37	0.086	0.702
4	4knhA	0.475	6.98	0.080	0.751
5	4kf7A	0.473	6.57	0.058	0.712
6	3zheA	0.473	5.44	0.031	0.636
7	4ifqA	0.470	7.03	0.069	0.751
8	5ijoJ	0.453	6.87	0.072	0.716
9	4uvkA	0.439	6.91	0.063	0.700
10	5ijnD	0.439	6.79	0.056	0.677

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.11	6	3ufdE	NUC	Rep, Mult	26,32,33,44,45,48,52,53,58
2	0.06	3	2c63B	III	Rep, Mult	82,89,171,172,204,205,206,239,242,246
3	0.06	3	1mz9B	VDY	Rep, Mult	113,116,120
4	0.04	2	3s8qB	NUC	Rep, Mult	41,42,43,44,45,46,55,56,57,58,61
5	0.04	2	2dysA	DCW	Rep, Mult	121,173,176
6	0.04	2	1o6oA	III	Rep, Mult	274,277,278,282,314,317,320,321
7	0.02	1	3wmmQ	CRT	Rep, Mult	251,254,258
8	0.02	1	3sjqC	PHU	Rep, Mult	41,42
9	0.02	1	3nteA	FE	Rep, Mult	48,52
10	0.02	1	1f59A	III	Rep, Mult	30,33,34,37,38,87,91,95
11	0.02	1	2iy4N	FE	Rep, Mult	126,129
12	0.02	1	1f59B	III	Rep, Mult	59,63,66,67,127,131
13	0.02	1	3rf3A	III	Rep, Mult	34,35,50
14	0.02	1	3mg9A	GHP	Rep, Mult	173,177
15	0.02	1	1u6g0	III	Rep, Mult	40,41,95,96

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1lf6A	0.361	7.06	0.042	0.570	3.2.1.3	NA
2	0.060	1z1wA	0.382	6.75	0.042	0.593	3.4.11.-	51
3	0.060	1rw9A	0.392	6.79	0.039	0.613	4.2.2.5	NA
4	0.060	2vn7A	0.384	6.61	0.061	0.591	3.2.1.3	NA
5	0.060	2ri9B	0.366	6.73	0.052	0.553	3.2.1.113	62,115
6	0.060	2j5wA	0.419	6.92	0.046	0.656	1.16.3.1	NA
7	0.060	1hcuB	0.363	6.68	0.062	0.556	3.2.1.113	NA
8	0.060	1fo2A	0.364	6.66	0.037	0.549	3.2.1.113	NA
9	0.060	1fmiA	0.366	6.83	0.037	0.564	3.2.1.113	NA
10	0.060	1llwA	0.364	7.50	0.037	0.609	1.4.7.1	NA
11	0.060	2dqmA	0.379	7.24	0.051	0.617	3.4.11.2	NA
12	0.060	2eabA	0.424	6.93	0.039	0.665	3.2.1.63	325
13	0.060	3dy5A	0.374	6.80	0.045	0.580	1.13.11.40,4.2.1.92	NA
14	0.060	2fhcA	0.380	7.32	0.073	0.628	3.2.1.41	48,53
15	0.060	1h54B	0.373	6.87	0.055	0.574	2.4.1.8	146
16	0.060	1l5jA	0.363	7.43	0.048	0.603	4.2.1.3	NA
17	0.060	3fg3A	0.297	7.96	0.049	0.521	4.2.1.92,1.13.11.40	NA
18	0.060	1ug9A	0.364	7.05	0.051	0.576	3.2.1.70	NA
19	0.060	1dl2A	0.370	6.74	0.056	0.568	3.2.1.113	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.16	0.801	2.88	0.09	0.88	4um2A	GO:0000184 GO:0000333 GO:0000723 GO:0000781 GO:0003677 GO:0004518 GO:0004519 GO:0004521 GO:0005634 GO:0005694 GO:0005697 GO:0005730 GO:0005737 GO:0005829 GO:0006406 GO:0016787 GO:0032210 GO:0035145 GO:0035303 GO:0042162 GO:0043021 GO:0046872 GO:0070034 GO:0070182 GO:0090305 GO:0090502 GO:1904354
1	0.10	0.312	7.30	0.03	0.51	1n1zA	GO:0000287 GO:0008152 GO:0009507 GO:0009536 GO:0010333 GO:0016829 GO:0016853 GO:0046211 GO:0046872 GO:0047926
2	0.07	0.662	3.84	0.07	0.78	1ya0B	GO:0000184 GO:0005634 GO:0005737 GO:0005829 GO:0006406 GO:0035303 GO:0042162 GO:0045111 GO:0051721
3	0.07	0.568	4.37	0.09	0.70	3zheB	GO:0000184 GO:0005634 GO:0005737
4	0.07	0.474	7.07	0.08	0.76	4ifqA	GO:0000059 GO:0005634 GO:0005643 GO:0006810 GO:0006999 GO:0015031 GO:0017056 GO:0044611 GO:0046822 GO:0051028
5	0.07	0.399	6.97	0.07	0.64	5hb4B	GO:0005634 GO:0005643 GO:0006810 GO:0015031 GO:0051028
6	0.07	0.475	6.98	0.08	0.75	4knhA	GO:0005634 GO:0005643 GO:0006810 GO:0015031 GO:0051028
7	0.06	0.439	6.79	0.06	0.68	5ijnD	GO:0000059 GO:0005634 GO:0005635 GO:0005643 GO:0005654 GO:0005737 GO:0006406 GO:0006409 GO:0006810 GO:0006913 GO:0007077 GO:0010827 GO:0015031 GO:0016020 GO:0016032 GO:0016925 GO:0017056 GO:0019083 GO:0031047 GO:0031965 GO:0034399 GO:0044611 GO:0051028 GO:0051292 GO:0075733 GO:1900034
8	0.06	0.476	6.92	0.05	0.75	4knhB	GO:0005634 GO:0005643 GO:0006810 GO:0015031 GO:0051028
9	0.06	0.330	6.86	0.04	0.52	5a31J	GO:0005634 GO:0005654 GO:0005680 GO:0005737 GO:0005813 GO:0005815 GO:0005819 GO:0005829 GO:0005856 GO:0005876 GO:0007049 GO:0007067 GO:0007088 GO:0008283 GO:0016567 GO:0031145 GO:0042787 GO:0043161 GO:0051301 GO:0051436 GO:0051437 GO:0051439 GO:0070979
10	0.06	0.298	5.36	0.08	0.40	2c0mC	GO:0000268 GO:0005052 GO:0005622 GO:0005737 GO:0005777 GO:0005778 GO:0005782 GO:0005829 GO:0006625 GO:0006810 GO:0008022 GO:0015031 GO:0016020 GO:0016558 GO:0016560 GO:0016561 GO:0019899 GO:0031267 GO:0043234 GO:0045046 GO:0047485 GO:0051262 GO:1901094
11	0.06	0.287	5.10	0.05	0.37	3cv0A	GO:0005052 GO:0005778 GO:0005829 GO:0016560
12	0.06	0.284	5.32	0.06	0.37	4eqfA	GO:0000268 GO:0005052 GO:0005737 GO:0005778 GO:0005829 GO:0016020 GO:0016560 GO:0030425 GO:0031267 GO:0042391 GO:0043235 GO:0043949 GO:0045185
13	0.06	0.298	5.14	0.07	0.39	2gw1A	GO:0005739 GO:0005741 GO:0005742 GO:0006626 GO:0006886 GO:0015266 GO:0015450 GO:0016020 GO:0016021 GO:0030150 GO:0030943 GO:0031307 GO:0045039
14	0.06	0.256	7.38	0.08	0.41	3mznA	GO:0003824 GO:0008152 GO:0008872 GO:0016829 GO:0046872
15	0.06	0.277	4.80	0.06	0.35	1w3bA	GO:0000123 GO:0000791 GO:0001933 GO:0001934 GO:0005547 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006041 GO:0006110 GO:0006486 GO:0006493 GO:0006915 GO:0007165 GO:0007584 GO:0008047 GO:0008134 GO:0008289 GO:0008375 GO:0010628 GO:0010801 GO:0016020 GO:0016262 GO:0016568 GO:0016740 GO:0016757 GO:0019904 GO:0030854 GO:0030900 GO:0031397 GO:0032868 GO:0032869 GO:0032922 GO:0033137 GO:0035020 GO:0042277 GO:0042588 GO:0043005 GO:0043025 GO:0043085 GO:0043981 GO:0043982 GO:0043984 GO:0043995 GO:0043996 GO:0045793 GO:0045862 GO:0045944 GO:0046626 GO:0046972 GO:0048015 GO:0048029 GO:0048312 GO:0048511 GO:0051571 GO:0060548 GO:0061087 GO:0070207 GO:0070208 GO:0070688 GO:0071222 GO:0071300 GO:0071333 GO:0080182 GO:0090315 GO:0090526 GO:0097237 GO:0097363 GO:1900038 GO:1900182 GO:1903428
16	0.06	0.280	4.84	0.05	0.36	1w3bB	GO:0000123 GO:0000791 GO:0001933 GO:0001934 GO:0005547 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0005829 GO:0005886 GO:0006041 GO:0006110 GO:0006486 GO:0006493 GO:0006915 GO:0007165 GO:0007584 GO:0008047 GO:0008134 GO:0008289 GO:0008375 GO:0010628 GO:0010801 GO:0016020 GO:0016262 GO:0016568 GO:0016740 GO:0016757 GO:0019904 GO:0030854 GO:0030900 GO:0031397 GO:0032868 GO:0032869 GO:0032922 GO:0033137 GO:0035020 GO:0042277 GO:0042588 GO:0043005 GO:0043025 GO:0043085 GO:0043981 GO:0043982 GO:0043984 GO:0043995 GO:0043996 GO:0045793 GO:0045862 GO:0045944 GO:0046626 GO:0046972 GO:0048015 GO:0048029 GO:0048312 GO:0048511 GO:0051571 GO:0060548 GO:0061087 GO:0070207 GO:0070208 GO:0070688 GO:0071222 GO:0071300 GO:0071333 GO:0080182 GO:0090315 GO:0090526 GO:0097237 GO:0097363 GO:1900038 GO:1900182 GO:1903428
17	0.06	0.174	6.07	0.04	0.25	4dwjB	GO:0000166 GO:0004798 GO:0005524 GO:0006233 GO:0006235 GO:0009165 GO:0016301 GO:0016310 GO:0016740 GO:0046939
18	0.06	0.203	3.63	0.04	0.24	2vyiA	GO:0005737 GO:0005829 GO:0016020 GO:0016032 GO:1903071 GO:1904288

Consensus prediction of GO terms

Molecular Function	GO:0043169	GO:0043565	GO:0019899	GO:0044877	GO:0004540	GO:0003723	GO:0004519
GO-Score	0.48	0.44	0.32	0.32	0.32	0.32	0.32
Biological Processes	GO:0000956	GO:0006405	GO:0071427	GO:0010467	GO:0016311	GO:0051028	GO:0019220	GO:0032205	GO:1904356	GO:2000278	GO:0007004	GO:1904353	GO:0090501
GO-Score	0.55	0.44	0.44	0.44	0.44	0.44	0.44	0.32	0.32	0.32	0.32	0.32	0.32
Cellular Component	GO:0044444	GO:0031981	GO:0043234	GO:0005697	GO:0098687
GO-Score	0.44	0.32	0.32	0.32	0.32

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.