I-TASSER results

I-TASSER results for job id Rv1125

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (414 residues)
MAGHRMAAVDAQFYWMSAKVPNDQFLLYAFDGEPTDLERAVAQVYRRARGCPGLGMRVQD
RGALAYPQWVPTPVQRDQLVCHDLADRSWQGCLAAVVGLASKQLDMRRMPWRLHVFTPVH
DVPGVSGLGTVAVMQFAHALGDGARASAMAAWLFGRPAAVPEIARSRAGFLPWRAAHAAR
AHLRLVRDTNAGLVAPGVGSRPPLSTNARPEGVRAVRTLLRRRSQLAGPTVTVTVLAAVS
TGLLGLLGGDVDTLGAEVPMAKPGVPRSYNHFGNVVVGLYPRLEPDERVRRIATDLANAR
RRFEHPAMLSADRAFAAVPAALLRWGVSQFDAEVRPVRVAGNTVVSSVYRGAADLSFGDA
PVVLTAGYPALSPAMGLTHGVHGIGDTVAISVHAAESAVSDIDAYMRLLDAALQ

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MAGHRMAAVDAQFYWMSAKVPNDQFLLYAFDGEPTDLERAVAQVYRRARGCPGLGMRVQDRGALAYPQWVPTPVQRDQLVCHDLADRSWQGCLAAVVGLASKQLDMRRMPWRLHVFTPVHDVPGVSGLGTVAVMQFAHALGDGARASAMAAWLFGRPAAVPEIARSRAGFLPWRAAHAARAHLRLVRDTNAGLVAPGVGSRPPLSTNARPEGVRAVRTLLRRRSQLAGPTVTVTVLAAVSTGLLGLLGGDVDTLGAEVPMAKPGVPRSYNHFGNVVVGLYPRLEPDERVRRIATDLANARRRFEHPAMLSADRAFAAVPAALLRWGVSQFDAEVRPVRVAGNTVVSSVYRGAADLSFGDAPVVLTAGYPALSPAMGLTHGVHGIGDTVAISVHAAESAVSDIDAYMRLLDAALQ
Prediction	CCCCCCCHHHHHHHHHCCCCCCCEEEEEEHCCCCCCHHHHHHHHHHHHHHCCHHHCEEEHCCCCCCCEEEHCCCCCCCEEEEHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHCCCCCCCCCCCEEEEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEHCCCCCCCCEEHCCEEEEEEEHCCCCCCCCEEEEEEEEHCCEEEEEEEHCHHHCCCHHHHHHHHHHHHC
Conf.Score	998778888988988567899868999997699999999999999999979666535664789999678877888865799867999999999999999988899999986999995767877678886899985446676589999999999868999888888877777657889999999999987655566777889987788876775457999999987559978999999999999999858998768998755899988888689998558899999999999999999999975555689999999986789999999987555677776557999779999976788687886887556567985689999997998999999775667899999999999979
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 300 320 340 360 380 400 \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| \| MAGHRMAAVDAQFYWMSAKVPNDQFLLYAFDGEPTDLERAVAQVYRRARGCPGLGMRVQDRGALAYPQWVPTPVQRDQLVCHDLADRSWQGCLAAVVGLASKQLDMRRMPWRLHVFTPVHDVPGVSGLGTVAVMQFAHALGDGARASAMAAWLFGRPAAVPEIARSRAGFLPWRAAHAARAHLRLVRDTNAGLVAPGVGSRPPLSTNARPEGVRAVRTLLRRRSQLAGPTVTVTVLAAVSTGLLGLLGGDVDTLGAEVPMAKPGVPRSYNHFGNVVVGLYPRLEPDERVRRIATDLANARRRFEHPAMLSADRAFAAVPAALLRWGVSQFDAEVRPVRVAGNTVVSSVYRGAADLSFGDAPVVLTAGYPALSPAMGLTHGVHGIGDTVAISVHAAESAVSDIDAYMRLLDAALQ
Prediction	766431000000001013621100000000124413053015102500440340021025335122010140531223100110325326201410140174304472000000002104424414310000000000000001001200220033323224144222222112012001100200120131222232333231313132122110231034026137000000000000000020146455200000100134564110000000010105241140043015105423543543213103200310000002200320233223431000000000011422000000102100000000121000000001303010001003400210430050035008
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280                 300                 320                 340                 360                 380                 400

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCHHHHHHHHHCCCCCCCEEEEEEHCCCCCCHHHHHHHHHHHHHHCCHHHCEEEHCCCCCCCEEEHCCCCCCCEEEEHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHCCCCCCCCCCCEEEEEEHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEEHCCCCCCCCEEHCCEEEEEEEHCCCCCCCCEEEEEEEEHCCEEEEEEEHCHHHCCCHHHHHHHHHHHHC
MAGHRMAAVDAQFYWMSAKVPNDQFLLYAFDGEPTDLERAVAQVYRRARGCPGLGMRVQDRGALAYPQWVPTPVQRDQLVCHDLADRSWQGCLAAVVGLASKQLDMRRMPWRLHVFTPVHDVPGVSGLGTVAVMQFAHALGDGARASAMAAWLFGRPAAVPEIARSRAGFLPWRAAHAARAHLRLVRDTNAGLVAPGVGSRPPLSTNARPEGVRAVRTLLRRRSQLAGPTVTVTVLAAVSTGLLGLLGGDVDTLGAEVPMAKPGVPRSYNHFGNVVVGLYPRLEPDERVRRIATDLANARRRFEHPAMLSADRAFAAVPAALLRWGVSQFDAEVRPVRVAGNTVVSSVYRGAADLSFGDAPVVLTAGYPALSPAMGLTHGVHGIGDTVAISVHAAESAVSDIDAYMRLLDAALQ

1q9jB

0.11

0.09

0.88

1.24

MUSTER

SVIRKLSHSEEVFAQYE----VFTSMTIQLRG-VIDVDALSDAFDALLETHPVLASHLEQSSD-GGWNLVADDLLHSGICVIDAEL----------------RLDQSVSLLHLQLIL--------REGGAELTLYLHHCMADGHHGAVLVDELFSRYTDAVTTDPGPITPQPTPLSMEAVLAQRGIERFMSVMYAYEIPALPQAVPVTRLWLSKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRAPTEATNLLGAASYLAEIGTDIVDLASDIVATLRADLANGVIQQSGL--------------HFGTAFEGTPPGLPPL--VFCTDATSFPTMRTPPGLEIEDIKGQFYCSISVPDLYSCAVYAGQLIIEHHGH---IAEPGKSLEAIRSLLC

1q9jB

0.10

0.09

0.88

1.45

dPPAS

SVIRKLSHSEEVFAQYE-----VFTSMTIQLRGVIDVDALSDAFDALLETHPVLASHLEQSSD-GGWNLVADDLLHSGICVIDAEL----------------RLDQSVSLLHLQLILR--------EGGAELTLYLHHCMADGHHGAVLVDELFSVTTGDPGPITPQPTPLSMEAVLAQRGIRKAERFMSVMYAYEIPATETPAVLAHPGLPSKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRFVLAPPVGAASYLAEIGPNTDIVDLASDIVATLRADLANGVIQQSGLHFGTAFEG----------------TPPGLPPLVFCTDATSFPTMRTPPGLEIEDIKGQFYCSISVPDLYSCAVYAGQLIIEHHGH---IAEPGKSLEAIRSLLC

1q9jB

0.10

0.09

0.86

2.01

wdPPAS

SVIRKLSHSEEVFAQYE-----VFTSMTIQLRGVIDVDALSDAFDALLETHPVLASHLEQSSD-GGWNLVADDLLHSGICVIDAE----------------LRLDQSVSLLHLQLILR--------EGGAELTLYLHHCMADGHHGAVLVDELFSVTTGDPGPITPQPTPLSMEAVLAQRGIRKAERFMSVMYAYEIPATETPAVLAHPGLPSKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRAPTEATNLLGAASYLAEINTDIVDLASDIVATLRADLANGVI-----------------GLHFGTAFEGTPPGLPPL--VFCTDFPTMRTP---PGLEIEDIKG---SISVPLDLYSCAVYAGQLIIEHHGH---IAEPGKSLEAIRSLLC

2vsqA

0.12

0.11

0.92

1.50

wMUSTER

FSKDQLSPMQEGMLFHAPGQSFYLEQITMKVKGSLNIKCLEESMNVIMDRYDVFRTVFIHE-KVKRPVQVVLKKRQFHIEEIDLTHLTTAKINEYKEQDKIRGFDLTRIPMRAAIFK-------KAEESFEWVWSYHHIILDGWCFGIVVQDLFKVYNALREQKPYSLPPVKWLEKQDKQASLRYWREYLEGFEGQTTFAEQRKKQKDGYEPSEAETKAFTELAKSQHTTLSTALQAVWSVLISRYQQSGD--LAFGTVVSGRPAEIKGHMVGLFINVVPRGITFNGLLKRLQEQSLQSEPHQY-------------VP-------LYDIQSQADQPKLIHIIVFENYPAKNEESSENGFDMVDVHVFE--KSNYDLNLMASP-GDEMLIKLAYNENVFDEILRLKSQLLTAIQ

1q9jB

0.11

0.10

0.86

2.08

wPPAS

SVIRKLSHSEEVFAQYE-----VFTSMTIQLRGVIDVDALSDAFDALLETHPVLASHLEQSSD-GGWNLVADDLLHSGICVIDAE----------------LRLDQSVSLLHLQLILR--------EGGAELTLYLHHCMADGHHGAVLVDELFSRTDAVTTGDPGPITPQPTPLSMEAVLAQRGERFMSVMYAYEIPATETPAVLAHPGLPQKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRAPTEATNLLGAASYLAEINTDIVDLASDIVATLRADLANGVI---QSGLHFGTAFEG--------------TPPGLPPLVFCTDFPTMRTP---PGLEIEDIKG--SISVPL-DLYSCAVYAGQLIIEHHGH---IAEPGKSLEAIRSLLC

1q9jB

0.11

0.09

0.88

3.90

dPPAS2

SVIRKLSHSEEVFAQYE----VFTSMTIQLRG-VIDVDALSDAFDALLETHPVLASHLEQSSD-GGWNLVADDLLHSGICVIDAEL----------------RLDQSVSLLHLQLILR--------EGGAELTLYLHHCMADGHHGAVLVDELFSVTTGDPGPITPQPTPLSMEAVLAQRGIRKAERFMSVMYAYEIPATETPAVLAHPGLPSKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRAPTEATNGAASYLAEIGPNTDIVDLASDIVATLRADLANGVIQQSGLH--------------FGTAFEGTPPGLPPL--VFCTDATSFPTMRTPPGLEIEDIKGQFYCSISVPDLYSCAVYAGQLIIEHHGH---IAEPGKSLEAIRSLLC

1q9jB

0.11

0.09

0.87

1.86

PPAS

SVIRKLSHSEEVFAQYE----VFTSMTIQLRG-VIDVDALSDAFDALLETHPVLASHLEQSSD-GGWNLVADDLLHSGICVIDAEL----------------RLDQSVSLLHLQLILR--------EGGAELTLYLHHCMADGHHGAVLVDELFSVTTGDPGPITPQPTPLSMEAVLAQRGIRKAERFMSVMYAYEIPATETPAVLAHPGLPQKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRAPTEATNGAASYLAEIGPNTDIVDLASDIVATLRADLANGVI-----------------GLHFGTAFEGTPPGLPPL--VFCTDATSFPTMRTPPGLEIEDIKGQFYCSISVPDLYSCAVYAGQLIIEHHGH---IAEPGKSLEAIRSLLC

5t3eA

0.10

0.09

0.93

3.35

Env-PPAS

--SEPLTEVQTAYMLGRNPQFESPQTYFEYET-ELDIARLSRSFQKVIQRHPMLRAVILPEG--KQQILRDVPEYEIEVESLVSMPPEAARLREERSRMIDHVFPLGQPLFELKAFQ-------LQEHTYLLCFRYDALLMDGASMNLVGQDLMHPDAQLPPLSFTMHIYDDMKRGTEYETAKAYWTNKLPDFPPAPSLAKDPAEIGTPNFQSKDKWLKLRRLAQDKQVTPSALLCTVYGEVLAFWSNQRR--LAINLTVFNRYPDEVEQSLILLDMDMDQKQPFFTKVEQTQSTLLDGLEHRH-------------YD--GVEFIRDYTRYHQMRPKAVMPIVFTSMLAGAGAFAWEE--IGSLRHIHARTPQVYLDNVVIEKNGELLVSWNYVEELFDAMESMFTQFVELLD

2xhgA

0.10

0.09

0.93

1.16

MUSTER

IIA-PLTPIQKWFFGKNFTNTGHNQSSVLYRPEGFDPKVIQSVMDKIIEHHDALRMVYQHENGNVVQHNRGLGGQLYDFFSYNLTAQPQQAIEAETQRLHSS-MNLQEPLVKVALFQT--------LHGDHLFLAIHHLVVDGISWRILFEDLATGYAQALAGQAISLPEKTQEYAADLLSEIPYWESLESQAKNVSLPKDYEVTDCKQKSHPEETEQLLKHANQAYQTEINDLLLAALGLAFAEWSKLA--QIVIHLEGHGREDIIEQ-SQYPVLLDLKQTAPLSDYIKLTKENMRKI---------------RKGIGYDILKHVTLPENRGSLSFRVQPEVTFN-ADMRTELFTRSGGNTLGADGKNNLSPESELNITGLIEGGELVLTFSYSSEQYRSIQQLSQSYQKHLL

2xhgA

0.09

0.95

1.26

dPPAS

VP---LTPIQKWFFGKNFTNTGHNQSSVLYRPEGFDPKVIQSVMDKIIEHHDALRMVYQHENGNVVQHNRGLGGQLYDFFSYNLAQPDVQQAIEAETQRLHSSMNLQEPLVKVALFQT--------LHGDHLFLAIHHLVVDGISWRILFEDLATGYAQALAGQAISLPEKTYANEADLLSEIPYWESLESQAKNVSLPKDYEVTDCKQKSHPEETEQLLKHANQAYQTEINDLLLAALGLAFAEWSKLAQ--IVIHLEGHGREDIIEQTSQYPVLLDLKQTAPLSDYIKLTKENMRKIPRKGIGYDILKHVTLPENRGSLSFRVQPEVTFDADMRTELFTRSPYSGGNTLGAD--------GKNNLSPESEVYTALNITGLIEGGELVLTFSYSSEQYRSIQQLSQSYQKHLL

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-0.82

Estimated TM-score = 0.61±0.14

Estimated RMSD = 8.8±4.6Å

Download Model 2

C-score=-1.57

Download Model 3

C-score=-2.83

Download Model 4

C-score=-1.69

Download Model 5

C-score=-3.51

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1q9jB	0.832	2.12	0.121	0.882
2	3fp0A	0.798	3.94	0.098	0.954
3	5iswA2	0.759	3.94	0.095	0.937
4	4jn3A	0.758	3.55	0.130	0.901
5	4zxhA	0.753	4.09	0.111	0.927
6	5t3eA	0.752	4.02	0.073	0.911
7	2xhgA	0.744	4.11	0.080	0.935
8	4znmA	0.741	4.02	0.135	0.908
9	4zxjA	0.740	4.08	0.118	0.913
10	3b2sA	0.738	4.25	0.076	0.920

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.10	5	2zbaA	ZBA	Rep, Mult	12,13,138,272,274,329,344,346,378,380
2	0.06	3	4kecA	4KE	Rep, Mult	24,136,138,142,143,147,328,346,378,380
3	0.06	3	5fanA	WCA	Rep, Mult	24,136,142,143,144,147,228,231,232,259,260,261,272,296,346,347,365
4	0.02	1	3sngA	ZN	Rep, Mult	120,138,142
5	0.02	1	1uduB	MG	Rep, Mult	105,189
6	0.02	1	1vf5S	III	Rep, Mult	243,244
7	0.02	1	1xl8B	MPD	Rep, Mult	234,286,289,290,293
8	0.02	1	1n38A	CH1	Rep, Mult	236,237,257,258,259,260
9	0.02	1	2v0nA	C2E	Rep, Mult	223,226,228
10	0.02	1	3qoqD	NUC	Rep, Mult	209,217
11	0.02	1	2y6pC	MG	Rep, Mult	31,142
12	0.02	1	3b2sA	B2S	Rep, Mult	19,22,113,138,198,274,346,377,379
13	0.02	1	1f4nB	CA	Rep, Mult	138,142
14	0.02	1	3fp0A	SWE	Rep, Mult	214,215,407
15	0.02	1	1xl7B	MPD	Rep, Mult	41,44,45,48
16	0.02	1	3d6aA	MG	Rep, Mult	253,254
17	0.02	1	2xr7A	MLC	Rep, Mult	221,229,231,232,233,234

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.092	2bghA	0.678	3.87	0.088	0.821	2.3.1.160	NA
2	0.060	1b5sA	0.358	4.22	0.108	0.442	2.3.1.12	NA
3	0.060	3b8kA	0.349	4.37	0.092	0.440	2.3.1.12	403
4	0.060	1ciaA	0.355	3.97	0.051	0.427	2.3.1.28	NA
5	0.060	1ybeA	0.356	6.54	0.056	0.563	2.4.2.11	385
6	0.060	1q23B	0.361	3.96	0.050	0.435	2.3.1.28	NA
7	0.060	1n1hA	0.353	7.06	0.037	0.594	2.7.7.48	49,167
8	0.060	2z8yD	0.360	7.57	0.069	0.626	1.2.7.4,1.2.99.2	137
9	0.060	1q6xA	0.625	4.42	0.055	0.790	2.3.1.6	NA
10	0.060	1xl7B	0.587	4.70	0.075	0.766	2.3.1.137	NA
11	0.060	1t7lA	0.342	7.09	0.060	0.575	2.1.1.14	NA
12	0.060	3cf4A	0.369	7.06	0.066	0.621	1.2.99.2	NA
13	0.060	1bf2A	0.357	6.77	0.056	0.585	3.2.1.68	NA
14	0.060	2ihwA	0.357	4.03	0.144	0.432	2.3.1.168	138
15	0.060	1jqkF	0.360	7.44	0.051	0.623	1.2.99.2	NA
16	0.060	3i39X	0.358	7.50	0.047	0.626	1.2.99.2	137,381
17	0.060	2debA	0.608	4.56	0.090	0.778	2.3.1.21	NA
18	0.060	2ii4A	0.358	4.18	0.152	0.440	2.3.1.168	260,346,366
19	0.060	3b9jC	0.301	7.12	0.030	0.510	1.17.3.2,1.17.1.4	NA
20	0.060	1eadA	0.355	4.42	0.130	0.442	2.3.1.12	NA

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.25	0.832	2.12	0.12	0.88	1q9jB	GO:0006629 GO:0008374 GO:0008610 GO:0016740 GO:0016746 GO:0016747 GO:0071770
1	0.16	0.798	3.94	0.10	0.95	3fp0A	GO:0016740
2	0.15	0.698	4.59	0.11	0.91	2jgpA	GO:0003824 GO:0008152 GO:0009058 GO:0016788 GO:0016874 GO:0017000 GO:0031177
3	0.15	0.770	3.48	0.13	0.91	4jn3B	GO:0003824 GO:0008152 GO:0031177
4	0.14	0.740	4.08	0.12	0.91	4zxjA	GO:0000166 GO:0003824 GO:0005524 GO:0005829 GO:0005886 GO:0008152 GO:0009058 GO:0009239 GO:0009366 GO:0016740 GO:0016779 GO:0016788 GO:0016874 GO:0031177 GO:0043041 GO:0047527
5	0.12	0.710	4.48	0.12	0.92	4tx3B	GO:0003824 GO:0008152 GO:0009058 GO:0016788 GO:0016874 GO:0031177
6	0.11	0.699	4.43	0.07	0.89	1l5aA	GO:0016880 GO:0019290
7	0.11	0.709	4.47	0.09	0.91	2vsqA	GO:0003824 GO:0008152 GO:0009058 GO:0016788 GO:0016874 GO:0017000 GO:0030435 GO:0031177
8	0.06	0.354	3.75	0.10	0.42	2i9dA	GO:0008811 GO:0016740
9	0.06	0.330	6.84	0.07	0.54	5d6jA	GO:0003824 GO:0008152 GO:0016874
10	0.06	0.339	7.10	0.05	0.56	4qflA	GO:0043190 GO:0055085
11	0.06	0.327	6.68	0.04	0.51	3e7wA	GO:0000166 GO:0003824 GO:0005524 GO:0005737 GO:0008152 GO:0016874 GO:0016876 GO:0047473 GO:0070395
12	0.06	0.318	6.85	0.04	0.52	4g36B	GO:0000166 GO:0003824 GO:0004497 GO:0005524 GO:0005777 GO:0008152 GO:0008218 GO:0016491 GO:0046872 GO:0047077 GO:0055114
13	0.06	0.298	7.21	0.03	0.50	4d56A	GO:0003824 GO:0008152
14	0.06	0.331	6.52	0.07	0.52	2d1sA	GO:0000166 GO:0003824 GO:0004497 GO:0005524 GO:0005777 GO:0008152 GO:0008218 GO:0016491 GO:0046872 GO:0047077 GO:0055114
15	0.06	0.326	7.04	0.02	0.54	3kxwA	GO:0003824 GO:0008152
16	0.06	0.310	7.23	0.07	0.53	4dg9A	GO:0000166 GO:0003824 GO:0008152
17	0.06	0.292	7.24	0.04	0.50	4eatB	GO:0003824 GO:0005524 GO:0008152 GO:0016405 GO:0016874
18	0.06	0.312	7.12	0.04	0.53	3ahvA	GO:0004553 GO:0004565 GO:0004567 GO:0005576 GO:0005975 GO:0008152 GO:0008422 GO:0016787 GO:0016798 GO:0033907 GO:0042803 GO:0042973 GO:0047668 GO:0047701 GO:0050224 GO:0080079 GO:0080083 GO:0102483 GO:1901657

Consensus prediction of GO terms

Molecular Function	GO:0016787	GO:0016747	GO:0031177
GO-Score	0.53	0.50	0.37
Biological Processes	GO:1901576	GO:0071766	GO:0044711	GO:0022607	GO:0044238
GO-Score	0.50	0.50	0.50	0.50	0.43
Cellular Component	GO:0009366	GO:0005886	GO:0005829
GO-Score	0.14	0.14	0.14

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.