I-TASSER results

I-TASSER results for job id Rv1078

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (240 residues)
MTEQPPPGGSYPPPPPPPGPSGGHEPPPAAPPGGSGYAPPPPPSSGSGYPPPPPPPGGGA
YPPPPPSAGGYAPPPPGPAIRTMPTESYTPWITRVLAAFIDWAPYVVLVGIGWVIMLVTQ
TSSCVTSISEYDVGQFCVSQPSMIGQLVQWLLSVGGLAYLVWNYGYRQGTIGSSIGKSVL
KFKVVSETTGQPIGFGMSVVRQLAHFIDAIICFVGFLFPLWDAKRQTLADKIMTTVCVPI

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MTEQPPPGGSYPPPPPPPGPSGGHEPPPAAPPGGSGYAPPPPPSSGSGYPPPPPPPGGGAYPPPPPSAGGYAPPPPGPAIRTMPTESYTPWITRVLAAFIDWAPYVVLVGIGWVIMLVTQTSSCVTSISEYDVGQFCVSQPSMIGQLVQWLLSVGGLAYLVWNYGYRQGTIGSSIGKSVLKFKVVSETTGQPIGFGMSVVRQLAHFIDAIICFVGFLFPLWDAKRQTLADKIMTTVCVPI
Prediction	CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEHC
Conf.Score	999999999999999999999999999999999988999999999999999999998888999999999999999887888877777776445689999999999999999999998765544445554555555445554578999999999999999999999986567898878766768997889998769999999999998799999999987547886558888768589869
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 \| \| \| \| \| \| \| \| \| \| \| \| MTEQPPPGGSYPPPPPPPGPSGGHEPPPAAPPGGSGYAPPPPPSSGSGYPPPPPPPGGGAYPPPPPSAGGYAPPPPGPAIRTMPTESYTPWITRVLAAFIDWAPYVVLVGIGWVIMLVTQTSSCVTSISEYDVGQFCVSQPSMIGQLVQWLLSVGGLAYLVWNYGYRQGTIGSSIGKSVLKFKVVSETTGQPIGFGMSVVRQLAHFIDAIICFVGFLFPLWDAKRQTLADKIMTTVCVPI
Prediction	865531464443444334644554434444344444443242244444444344334444323212334323323233334444434234233200002113313332333233312233333123334333333333433313132233333333222111120133243111001200301003264443021110020121333213313311000000542000002004021155
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEHC
MTEQPPPGGSYPPPPPPPGPSGGHEPPPAAPPGGSGYAPPPPPSSGSGYPPPPPPPGGGAYPPPPPSAGGYAPPPPGPAIRTMPTESYTPWITRVLAAFIDWAPYVVLVGIGWVIMLVTQTSSCVTSISEYDVGQFCVSQPSMIGQLVQWLLSVGGLAYLVWNYGYRQGTIGSSIGKSVLKFKVVSETTGQPIGFGMSVVRQLAHFIDAIICFVGFLFPLWDAKRQTLADKIMTTVCVPI

2miiA

0.15

0.10

0.68

1.28

wPPAS

MVGQREPAPVEEVKPAPEQPAEPQQPVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQGADGVTAGSDSVNNRTNGSLN--------AAEATETLRNALANN--FTLVSAQQLSMAKQQLGLSPQDSLGTRSKAIGIARNV----------------------GAH-SS-ASGMQLMLVQTGE------------------------IIWS----SQQ--------------

5gaoE

0.06

0.05

0.86

1.26

dPPAS2

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-------AAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK--------------------------

5gaoE

0.05

0.91

1.30

PPAS

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAARAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTKEAYKKLSQKFHGTKSNKK---------------------

1mv3A

0.10

0.06

0.60

1.20

wPPAS

EVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLP-PGFMFKVQA-QHD-------------------------------------------------------------Y-------TATQLG-----VLVIPFQNPEEQD--------------------GWLMGVKESDWNQHKKLEKCR-TERV

1g9lA

0.06

0.03

0.55

1.11

dPPAS2

------------GPLGSAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERAMHPTLAGKITGMLLESELLHMLESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV------------------------------------------------------------------------------------------------

1mv3A

0.15

0.12

0.78

1.26

PPAS

HEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQPAEASEVAGGTQPAAGAQEPGETAASEAAS------------------------SSLPAVVVETFPATVNGTVEGGSGA-------------------------GRLDLPPGF---MFKVQAQTDTDELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKKLEKCRGVFPE

3t9nA1

0.08

0.04

0.53

1.03

wPPAS

------------------------------------------------------------------------------------------YDIKAVKFLLDVLKILIIAFIGIKFADFLIYRFY-----KLYSKSKIQLPQRKIDTLTSLTKNAVRYIIYFLAGASILKLFNIDMTSLLAVAGIG----S--LAIGFGAQNLVKDMISGFFIIFE-----FS-------DTINGITVEEI

3zifN

0.09

0.04

0.45

1.06

dPPAS2

-------------------EGRIYVPYVTARLPKWSGSVQDKTGSNMLGGVVLPPNSQAHRTETVGTEATRDNLHAEGARRPEDQTPYMILVEDSLGGLKRRMDLLEESNQQLLATLNRLRTGLAA------------------------------------------------------------------------------------------------------------------

4bedB2

0.09

0.95

1.09

PPAS

MADHGPNGGNPPMCPMPDGKNYSCCTHGMAGARVGLPYWDGTTAFTALPTFVTDEEDNPFHHGHIDYLGVDTTRSPRDKLFNDPERGSESFFYRQVLLALEQTDFCQFEVQFEITHNAIHSWTGGLTPYGMSTLEYTTYDPLFWLHHANTDRIWAIWQALQEYRGLPYDHANCEIQAMKRPLR---SDPINHNAFTHSNAK-PTDVFEYSRF-LRFHGMTIKKLEHELEKQKEE------

4bw5A2

0.09

0.05

0.58

0.95

wPPAS

--------------------------------------------------------------------------------NSSHNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGK---SIARVEKVFRKIRVISTILFILAGCIVFVTIPAVIFKYIEGWT-TTVG---FGDFVAGGNAGINYRE-----WYKPLVWFWILVGLAYFAAVLS----MIGDWLR-----VL

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.55

Estimated TM-score = 0.24±0.07

Estimated RMSD = 17.1±2.8Å

Download Model 2

C-score=-4.68

Download Model 3

C-score=-5.00

Download Model 4

C-score=-4.49

Download Model 5

C-score=-4.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	2cse1	0.484	5.44	0.055	0.762
2	4xhjA	0.476	5.55	0.043	0.767
3	3m1cA	0.471	5.60	0.061	0.758
4	4w8yA	0.455	5.86	0.041	0.783
5	3phfA	0.439	5.88	0.028	0.746
6	2pywA	0.434	5.37	0.054	0.688
7	5l43A2	0.430	5.45	0.037	0.688
8	2pulA	0.428	5.70	0.049	0.713
9	3ja4A	0.428	5.84	0.028	0.717
10	3ayfA	0.426	5.87	0.049	0.717

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.05	3	1n38A	CH1	Rep, Mult	218,219,226,231,232,240
2	0.04	2	3gwoA	JEF	Rep, Mult	162,166
3	0.04	2	3jzmE	MG	Rep, Mult	172,177
4	0.04	2	1nkzA	RG1	Rep, Mult	226,231,232
5	0.04	2	2z8yD	SF4	Rep, Mult	230,232,233,238,240
6	0.04	2	4yfkC	4C6	Rep, Mult	203,207
7	0.02	1	3mrzX	MG	Rep, Mult	183,184
8	0.02	1	3i8gQ	MG	Rep, Mult	225,228
9	0.02	1	4tt0A	IOD	Rep, Mult	158,161
10	0.02	1	1oczB	HEA	Rep, Mult	117,154
11	0.02	1	2pywB	EDO	Rep, Mult	116,120,148,151
12	0.02	1	4bo7D	J2T	Rep, Mult	92,96,149,153
13	0.02	1	3ehbB	HEA	Rep, Mult	151,228
14	0.02	1	3sgf0	MG	Rep, Mult	200,201
15	0.02	1	3a9qG	CA	Rep, Mult	230,234
16	0.02	1	3perB	PO4	Rep, Mult	109,112,157
17	0.02	1	3spaA	III	Rep, Mult	107,111,175,180
18	0.02	1	4ev6E	MG	Rep, Mult	117,217
19	0.02	1	1mz9B	VDY	Rep, Mult	151,154,158
20	0.02	1	2c1hA	MG	Rep, Mult	94,146

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1n1hA	0.482	5.41	0.055	0.758	2.7.7.48	93
2	0.060	1ox0A	0.390	5.95	0.041	0.667	2.3.1.41	NA
3	0.060	1yq3A	0.389	5.48	0.040	0.629	1.3.5.1	NA
4	0.060	3cf4A	0.393	6.20	0.023	0.704	1.2.99.2	226
5	0.060	1h16A	0.389	5.56	0.023	0.604	2.3.1.54	NA
6	0.060	3b98A	0.391	6.06	0.036	0.675	5.3.99.4	NA
7	0.060	2vz8B	0.394	5.93	0.039	0.688	2.3.1.85	NA
8	0.060	2pnrB	0.386	5.44	0.059	0.613	2.7.11.2	NA
9	0.060	2q1fA	0.406	5.28	0.049	0.625	4.2.2.21	NA
10	0.060	2jfdA	0.284	6.47	0.021	0.529	2.3.1.85	92
11	0.060	2vz8A	0.392	5.85	0.049	0.675	2.3.1.85	NA
12	0.060	2vz9B	0.340	5.98	0.068	0.583	2.3.1.85	NA
13	0.060	1e7pA	0.375	5.50	0.046	0.613	1.3.99.1	NA
14	0.060	1b0pA	0.395	5.87	0.047	0.667	1.2.7.1	NA
15	0.060	3crlB	0.401	5.63	0.069	0.662	2.7.11.2	217,228
16	0.060	2r7oA	0.390	5.30	0.026	0.625	2.7.7.48	NA
17	0.060	3cskA	0.407	5.68	0.055	0.654	3.4.14.4	218
18	0.060	1z0hB	0.329	6.22	0.057	0.567	3.4.24.69	164
19	0.060	2pnqB	0.393	6.03	0.036	0.700	3.1.3.43	230

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.07	0.471	5.60	0.06	0.76	3m1cA	GO:0016020 GO:0016021 GO:0019012 GO:0019031 GO:0019064 GO:0020002 GO:0033644 GO:0039663 GO:0044174 GO:0044175 GO:0046718 GO:0055036
1	0.06	0.324	5.92	0.04	0.54	2xqyA	GO:0016020 GO:0016021 GO:0019012 GO:0019031 GO:0019064 GO:0020002 GO:0033644 GO:0039663 GO:0046718 GO:0055036
2	0.06	0.476	5.55	0.04	0.77	4xhjA	GO:0016020 GO:0016021 GO:0019012 GO:0019031 GO:0019064 GO:0020002 GO:0033644 GO:0039663 GO:0044174 GO:0044175 GO:0046718 GO:0055036
3	0.06	0.321	6.39	0.04	0.60	4bhtA	GO:0004354 GO:0005737 GO:0005829 GO:0006520 GO:0006537 GO:0016491 GO:0016639 GO:0042802 GO:0055114
4	0.06	0.311	6.22	0.05	0.57	2zwiB	GO:0016740 GO:0016757 GO:0046872
5	0.06	0.312	6.30	0.05	0.57	3f41A	GO:0004725 GO:0016158 GO:0016311 GO:0016787 GO:0016791 GO:0035335
6	0.06	0.308	6.02	0.06	0.53	3r3jA	GO:0004354 GO:0006520 GO:0006537 GO:0016491 GO:0016639 GO:0020011 GO:0055114
7	0.06	0.285	6.70	0.04	0.54	4zchA	GO:0002376 GO:0005102 GO:0005125 GO:0005164 GO:0005576 GO:0005615 GO:0005654 GO:0005737 GO:0005829 GO:0005886 GO:0006955 GO:0007165 GO:0008283 GO:0008284 GO:0016020 GO:0016021 GO:0030890 GO:0033209 GO:0043488 GO:0048298 GO:0048471 GO:0070062
8	0.06	0.445	5.57	0.07	0.73	3phf1	GO:0016020 GO:0016021 GO:0019012 GO:0019031 GO:0019064 GO:0020002 GO:0033644 GO:0039663 GO:0046718 GO:0055036
9	0.06	0.297	6.26	0.02	0.53	3lstA	GO:0008168 GO:0008171 GO:0032259
10	0.06	0.287	6.53	0.04	0.55	1r4qL	GO:0016787 GO:0017148 GO:0030598
11	0.06	0.286	5.51	0.05	0.45	2wwdA	GO:0003796 GO:0005975 GO:0008152 GO:0009253 GO:0016787 GO:0016798 GO:0016998
12	0.06	0.278	6.38	0.04	0.51	1f0kA	GO:0005886 GO:0005975 GO:0007049 GO:0008360 GO:0009252 GO:0016020 GO:0016740 GO:0016757 GO:0016758 GO:0030259 GO:0050511 GO:0051301 GO:0051991 GO:0071555
13	0.06	0.338	5.98	0.05	0.58	1v5vA	GO:0004047 GO:0006546 GO:0008483 GO:0016740 GO:0019464 GO:0032259
14	0.06	0.268	5.84	0.06	0.45	1p2fA	GO:0000160 GO:0003677 GO:0005622 GO:0006351 GO:0006355 GO:0046872
15	0.06	0.248	6.45	0.06	0.46	2eg4A	GO:0004792 GO:0016740 GO:0046872
16	0.06	0.318	6.41	0.04	0.58	4qrzA	GO:0005215 GO:0006810
17	0.06	0.484	5.44	0.06	0.76	2cse1	GO:0000166 GO:0001172 GO:0003723 GO:0003968 GO:0016740 GO:0016779 GO:0019012 GO:0019013 GO:0019028 GO:0019079 GO:0032774
18	0.06	0.336	5.24	0.02	0.51	2wbeC	GO:0000166 GO:0000278 GO:0000922 GO:0001578 GO:0003774 GO:0003777 GO:0005524 GO:0005634 GO:0005737 GO:0005783 GO:0005794 GO:0005813 GO:0005818 GO:0005819 GO:0005856 GO:0005871 GO:0005873 GO:0005874 GO:0005875 GO:0007018 GO:0007030 GO:0007049 GO:0007052 GO:0007059 GO:0007067 GO:0007100 GO:0008017 GO:0008574 GO:0009306 GO:0022008 GO:0031535 GO:0042998 GO:0045169 GO:0045478 GO:0048085 GO:0051289 GO:0051298 GO:0051299 GO:0051301 GO:0072686 GO:0090307 GO:0097431 GO:1990498

Consensus prediction of GO terms

Molecular Function	GO:0046872	GO:0016757	GO:0042802	GO:0004354
GO-Score	0.06	0.06	0.06	0.06
Biological Processes	GO:0044800	GO:0046718
GO-Score	0.36	0.36
Cellular Component	GO:0033643	GO:0036338	GO:0044218
GO-Score	0.36	0.36	0.36

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.