I-TASSER results

I-TASSER results for job id Rv1073

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Input Sequence in FASTA format

>protein (283 residues)
MGAQPFIGSEALAAGLISWHELGKYYTAIMPNVYLDKRLKPSLRQRVIAAWLWSGRKGVI
AGASASALHGAKWVDDHALVELIWRNARAPNGVRTKDELLLDGEVQRLCGLTVTTVERTA
FDLGRRPPLGQAITRLDALANATDFKINDVRELARKHPHTRGLRQLDKALDLVDPGAQSP
KETWLRLLLINAGFPRPSTQIPLLGVYGHPKYFLDMGWEDIMLAVEYDGEQHRLSRDQFV
KDVERLEYIRRAGWTHIRVLADHKGPDVVRRVRQAWDTLTSRR

Predicted Secondary Structure

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MGAQPFIGSEALAAGLISWHELGKYYTAIMPNVYLDKRLKPSLRQRVIAAWLWSGRKGVIAGASASALHGAKWVDDHALVELIWRNARAPNGVRTKDELLLDGEVQRLCGLTVTTVERTAFDLGRRPPLGQAITRLDALANATDFKINDVRELARKHPHTRGLRQLDKALDLVDPGAQSPKETWLRLLLINAGFPRPSTQIPLLGVYGHPKYFLDMGWEDIMLAVEYDGEQHRLSRDQFVKDVERLEYIRRAGWTHIRVLADHKGPDVVRRVRQAWDTLTSRR
Prediction	CCCCCCCHHHHHHHCCHHHHHHHCCEEEHCCCEEHCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCEEEHCCCCCCCCCEEEEEHCCCCCCEEEHCCEEHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEHCCCCCEEEEEEEEHCCEEEEEEHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEHCCCCCHHHHHHHHHHHHHHHHCC
Conf.Score	9988634899999887468887698688579889779999899999999999968985774489999989988889985899879999999779998578888589987888669999999999858889999999999986899899999999978887547999999997556678989999999999989998877589987888768887678779589999869877789999999999999999989999999766577699999999999998669
	H:Helix; S:Strand; C:Coil

Predicted Solvent Accessibility

Sequence	20 40 60 80 100 120 140 160 180 200 220 240 260 280 \| \| \| \| \| \| \| \| \| \| \| \| \| \| MGAQPFIGSEALAAGLISWHELGKYYTAIMPNVYLDKRLKPSLRQRVIAAWLWSGRKGVIAGASASALHGAKWVDDHALVELIWRNARAPNGVRTKDELLLDGEVQRLCGLTVTTVERTAFDLGRRPPLGQAITRLDALANATDFKINDVRELARKHPHTRGLRQLDKALDLVDPGAQSPKETWLRLLLINAGFPRPSTQIPLLGVYGHPKYFLDMGWEDIMLAVEYDGEQHRLSRDQFVKDVERLEYIRRAGWTHIRVLADHKGPDVVRRVRQAWDTLTSRR
Prediction	7773212011012223021230344022011001014623320200010001104330000130001014041054432010113431322100000211335301413201002002000000012223200200100122341223002302442422220222220221122333321002000001313112221001021333320010000023120000021433244351144034125203613010000015222540032023005315748
	Values range from 0 (buried residue) to 8 (highly exposed residue)

Predicted Normalized B-facotr

(B-factor is a value to indicate the extent of the inherent thermal mobility of residues/atoms in proteins. In I-TASSER, this value is deduced from threading template proteins from the PDB in combination with the sequence profiles derived from sequence databases. The reported B-factor profile in the figure below corresponds to the normalized B-factor of the target protein, defined by B=(B'-u)/s, where B' is the raw B-factor value, u and s are respectively the mean and standard deviation of the raw B-factors along the sequence. (Click here to read more about predicted normalized B-factor)

Top 10 threading templates used by I-TASSER

Rank

PDB
Hit

Iden1

Iden2

Cov

Norm.
Zscore

Download
Align.

                  20                  40                  60                  80                 100                 120                 140                 160                 180                 200                 220                 240                 260                 280

                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |                   |

Sec.Str
Seq

CCCCCCCHHHHHHHCCHHHHHHHCCEEEHCCCEEHCCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCEEEHCCCCCCCCCEEEEEHCCCCCCEEEHCCEEHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEHCCCCCEEEEEEEEHCCEEEEEEHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEHCCCCCHHHHHHHHHHHHHHHHCC
MGAQPFIGSEALAAGLISWHELGKYYTAIMPNVYLDKRLKPSLRQRVIAAWLWSGRKGVIAGASASALHGAKWVDDHALVELIWRNARAPNGVRTKDELLLDGEVQRLCGLTVTTVERTAFDLGRRPPLGQAITRLDALANATDFKINDVRELARKHPHTRGLRQLDKALDLVDPGAQSPKETWLRLLLINAGFPRPSTQIPLLGVYGHPKYFLDMGWEDIMLAVEYDGEQHRLSRDQFVKDVERLEYIRRAGWTHIRVLADHKGPDVVRRVRQAWDTLTSRR

4oq2A1

0.15

0.07

0.46

1.46

wdPPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------HHHHHEFSKTDYILRALSKISHKRWEHYIINRVVHTLDDPDIEFVCQQCIRKEGHLGKIYLADLLFPQLNLYLEIDE-AHHDSNDARKADAVRRLDVEATGFQEERIPASNITSEVNKLVDEFVRLVKDKK

4oq2A1

0.15

0.07

0.46

1.43

wPPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------HHHHHEFSKTDYILRALSKISHKRWEHYIINRVVHTLDDPDIEFVCQQCIRKEGHLGKIYLADLLFPQLNLYLEIDE-AHHDSNDARKADAVRRLDVEATGFQEERIPASNITSEVNKLVDEFVRLVKDKK

4oq2A1

0.15

0.07

0.46

1.04

dPPAS2

---------------------------------------------------------------------------------------------------------------------------------------------------------HHHHHEFSKTDYILRALSKISHKREHYIINRVVHTLDDPDIEFVCQQCIRKEGHLGKIYLADLLFPQLNLYLEIDEAHHDSNDARKADAVRRLDIVEATGFQEERIPASNITSEVNKLVDEFVRLVKDKK

3hrlA

0.21

0.07

0.32

1.27

Env-PPAS

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ASEAEAKLWQHLRAGRLNGYKFRRQQPGN-----YIVDFCVT-PKLIVEADGVY----------DHARTVYLNSLGFTVLRFWNHEILQQTNDVLAEILRVLQELE

4parB1

0.14

0.06

0.44

1.13

wdPPAS

------------------------------------------------------------------------------------------------------------------------------------------------------------ASSDLTDYVIRQLGRTKNKRYEAYVVSRIIHLLNDFTLKFVTQQFVRLSNKK--IALTDLYFPQLGIHIEVDEGHHFLRISQTESDAMREEDISITGHKIFRVNVFKNQENIHQQIDKIIEEIKTAK

4parB1

0.15

0.06

0.43

1.12

wPPAS

----------------------------------------------------------------------------------------------------------------------------------------------------------------ASSDLTDYVIRQLNKRYEAYVVSRIIHLLNDFTLKFVTQQFVRLSNKK--IALTDLYFPQLGIHIEVDEGHHFLRISQTESDAMREEDISITGHKIFRVNVFKNQENIHQQIDKIIEEIKTAK

4oq2A1

0.17

0.08

0.46

1.01

Env-PPAS

---------------------------------------------------------------------------------------------------------------------------------------------------------HHHHHEFSKTDYILRALSISHKRWEHYIINRVVHTLDDPDIEFVCQQCIRKEGHLGKIYLADLLFPQLNLYLEIDEA-HHDSNDARKADAVRRLDIVEAGFQEERIPASNITSEVNKLVDEFVRLVKDKK

3hrlA

0.24

0.07

0.30

0.97

wPPAS

---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ASEAEAKLWQHLRAGRLNG-RRQQP-----GN--YIVDF--VTPKLIVEADGV----------YDHARTVYLNSLGFTVLRFWNHEILQQTNDVLAEILRVLQELE

4bqnA

0.09

0.08

0.94

0.72

MUSTER

MAQRKKYQAPALAKMLNSCPTFARDWELVE---MEPCFVASEEQIDRHLAETIPKLDLFLYQP-------VSEGYRGEKYSSVFLRNSMPPGGNALSVYMHWETVNSPYGLPPHPEALIAGAVVMDVDKETYLRHLEEIGASLRIDIDEIESWCVDEGKQIDISVTDFILANCRQKRAALMREIAARCMLALGYTYSDISFDQNLDPLDVT--KMSLYPIYRDCFDFSELNRMNEYQVLYKKKAYEPYLLEQ----FEWFERSPKADVSAFFDRVAANRRWVR

4oq2A1

0.14

0.06

0.46

0.60

dPPAS

--------------------------------------------------------------------------------------------------------------------------------------------------------HHHHHEFSKTDYILRALSKISHKRWEHYIINRVVHTLDDPDIEFVCQQCIRKEGHLGKIYLADLLFPQLNLYLEIDEAHHDSNDARKADAVRRLDIVEATGFQEERIPASNITLSEVNKLVDEFVRLVKDK

(a)	Iden1 is the number of template residues identical to query divided by number of aligned residues.
(b)	Iden2 is the number of template residues identical to query divided by query sequence length.
(c)	Cov is equal the number of aligned template residues divided by query sequence length.
(d)	Norm. Zscore is the normalized Z-score of the threading alignments. A Normalized Z-score >1 means a good alignment.
(e)	Download Align. list the threading program used to identify the template provide the 3D structure of aligned regions of threading templates.

Top 5 final models predicted by I-TASSER

(For each target, I-TASSER simulations generate a large ensemble of structural conformations, called decoys. To select the final models, I-TASSER uses the SPICKER program to cluster all the decoys based on the pair-wise structure similarity, and reports up to five models which corresponds to the five largest structure clusters. The confidence of each model is quantitatively measured by C-score that is calculated based on the significance of threading template alignments and the convergence parameters of the structure assembly simulations. C-score is typically in the range of [-5, 2], where a C-score of higher value signifies a model with a high confidence and vice-versa. TM-score and RMSD are estimated based on C-score and protein length following the correlation observed between these qualities. Since the top 5 models are ranked by the cluster size, it is possible that the lower-rank models have a higher C-score in rare cases. Although the first model has a better quality in most cases, it is also possible that the lower-rank models have a better quality than the higher-rank models as seen in our benchmark tests. If the I-TASSER simulations converge, it is possible to have less than 5 clusters generated. This is usually an indication that the models have a good quality because of the converged simulations.)

More about C-score

Local structure accuracy of first model

Download Model 1

C-score=-4.90

Estimated TM-score = 0.22±0.06

Estimated RMSD = 18.6±2.2Å

Download Model 2

C-score=-5.00

Download Model 3

C-score=-5.00

Download Model 4

C-score=-5.00

Download Model 5

C-score=-5.00

Proteins structureally close to the target in PDB (as identified by TM-align

(After the structure assembly simulation, I-TASSER uses the TM-align structural alignment program to match the first I-TASSER model to all structures in the PDB library. This section reports the top 10 proteins from the PDB that have the closest structural similarity, i.e. the highest TM-score, to the predicted I-TASSER model. Due to the structural similarity, these proteins often have similar function to the target. However, users are encouraged to use the data in the next section 'Predicted function using COACH' to infer the function of the target protein, since COACH has been extensively trained to derive biological functions from multi-source of sequence and structure features which has on average a higher accuracy than the function annotations derived only from the global structure comparison.)

Top 10 Identified stuctural analogs in PDB

Rank	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov
1	1l5jA	0.438	6.44	0.050	0.756
2	1uyrA2	0.436	6.15	0.072	0.731
3	5i6fA	0.434	6.16	0.074	0.728
4	3ff6A	0.434	6.22	0.075	0.728
5	4oq2A	0.431	1.84	0.130	0.463
6	3eh2A	0.431	5.47	0.070	0.661
7	3efoB	0.430	5.47	0.070	0.664
8	4cu6A	0.428	6.42	0.056	0.753
9	3eh1A	0.426	5.22	0.044	0.647
10	1krcC	0.425	5.99	0.056	0.693

(a)	Query structure is shown in cartoon, while the structural analog is displayed using backbone trace.
(b)	Ranking of proteins is based on TM-score of the structural alignment between the query structure and known structures in the PDB library.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of the alignment by TM-align and is equal to the number of structurally aligned residues divided by length of the query protein.

Predicted function using COACH

(This section reports biological annotations of the target protein by COACH based on the I-TASSER structure prediction. COACH is a meta-server approach that combines multiple function annotation results from the COFACTOR, TM-SITE and S-SITE programs.)

Ligand binding sites

Rank	C-score	Cluster size	PDB Hit	Lig Name	Download Complex	Ligand Binding Site Residues
1	0.14	9	3tv5C	RCP	Rep, Mult	115,120,121,124
2	0.11	7	1stxA	MN	Rep, Mult	178,215,226,227
3	0.11	7	2jfrA	MG	Rep, Mult	228,229
4	0.02	1	5esrA	CO	Rep, Mult	182,199
5	0.02	1	4y8aB	ZN	Rep, Mult	226,256
6	0.02	1	3pgqB	GY3	Rep, Mult	119,120,123,136,139
7	0.02	1	3fozB	CA	Rep, Mult	280,283
8	0.02	1	2x24B	X24	Rep, Mult	185,188,189
9	0.02	1	4e45D	ZN	Rep, Mult	238,242
10	0.02	1	3ff6C	RCP	Rep, Mult	67,68,70,71
11	0.02	1	3h0qB	B37	Rep, Mult	62,63,65,68
12	0.02	1	1brrB	ARC	Rep, Mult	227,279
13	0.02	1	5cg6A	MG	Rep, Mult	274,277

	Download the all possible binding ligands and detailed prediction summary.
	Download the templates clustering results.
(a)	C-score is the confidence score of the prediction. C-score ranges [0-1], where a higher score indicates a more reliable prediction.
(b)	Cluster size is the total number of templates in a cluster.
(c)	Lig Name is name of possible binding ligand. Click the name to view its information in the BioLiP database.
(d)	Rep is a single complex structure with the most representative ligand in the cluster, i.e., the one listed in the Lig Name column. Mult is the complex structures with all potential binding ligands in the cluster.

Enzyme Commission (EC) numbers and active sites

Rank	Cscore^EC	PDB Hit	TM-score	RMSD^a	IDEN^a	Cov	EC Number	Active Site Residues
1	0.060	1s9rA	0.408	5.49	0.045	0.629	3.5.3.6	NA
2	0.060	3kx2B	0.408	6.06	0.058	0.678	3.6.4.13	NA
3	0.060	3ff6A	0.434	6.22	0.075	0.728	6.4.1.2,6.3.4.14	48
4	0.060	1clcA	0.408	6.16	0.057	0.682	3.2.1.4	NA
5	0.060	1kb0A	0.405	6.20	0.069	0.685	1.1.99.-	NA
6	0.060	1ua4A	0.404	6.69	0.090	0.728	2.7.1.147	NA
7	0.060	1xc6A	0.337	6.90	0.044	0.629	3.2.1.23	NA
8	0.060	1m7jA	0.413	5.99	0.021	0.685	3.5.1.81	NA
9	0.060	1sllA	0.412	6.38	0.023	0.714	4.2.2.15	257
10	0.060	1h54B	0.406	6.78	0.057	0.746	2.4.1.8	NA
11	0.060	1ie7C	0.418	6.39	0.056	0.714	3.5.1.5	NA
12	0.060	1sliA	0.412	6.00	0.042	0.682	4.2.2.15,3.2.1.18	NA
13	0.060	1vrgA	0.402	6.24	0.048	0.685	6.4.1.2	139
14	0.060	1a5kC	0.427	6.11	0.056	0.703	3.5.1.5	271
15	0.060	1on3E	0.410	6.27	0.036	0.693	2.1.3.1	NA
16	0.060	1yniB	0.378	6.77	0.055	0.689	3.5.3.23	186
17	0.060	1uyvA	0.429	6.12	0.039	0.714	6.3.4.14,6.4.1.2	NA
18	0.060	1gkpA	0.407	6.28	0.040	0.693	3.5.2.2	NA
19	0.060	1r7aA	0.383	6.52	0.076	0.675	2.4.1.7	226,262

(a)	Cscore^EC is the confidence score for the EC number prediction. Cscore^EC values range in between [0-1]; where a higher score indicates a more reliable EC number prediction.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.

Gene Ontology (GO) terms

Rank	Cscore^GO	TM-score	RMSD^a	IDEN^a	Cov	PDB Hit	Associated GO Terms
Homologous GO templates in PDB
0	0.07	0.412	5.98	0.04	0.67	2a7sA	GO:0000166 GO:0003989 GO:0004658 GO:0005524 GO:0005618 GO:0005886 GO:0009317 GO:0015977 GO:0016874 GO:0043234
1	0.07	0.427	6.00	0.05	0.71	4wyoB	GO:0000166 GO:0003824 GO:0003989 GO:0004075 GO:0005524 GO:0005737 GO:0005783 GO:0005789 GO:0006606 GO:0006629 GO:0006631 GO:0006633 GO:0006998 GO:0008152 GO:0016020 GO:0016874 GO:0042759 GO:0046872 GO:2001295
2	0.06	0.429	6.12	0.04	0.71	1uyvA	GO:0000166 GO:0003824 GO:0003989 GO:0004075 GO:0005524 GO:0005737 GO:0005783 GO:0005789 GO:0006606 GO:0006629 GO:0006631 GO:0006633 GO:0006998 GO:0008152 GO:0016020 GO:0016874 GO:0042759 GO:0046872 GO:2001295
3	0.06	0.423	6.49	0.04	0.74	1uyrA	GO:0000166 GO:0003824 GO:0003989 GO:0004075 GO:0005524 GO:0005737 GO:0005783 GO:0005789 GO:0006606 GO:0006629 GO:0006631 GO:0006633 GO:0006998 GO:0008152 GO:0016020 GO:0016874 GO:0042759 GO:0046872 GO:2001295
4	0.06	0.434	6.47	0.06	0.76	4asiC	GO:0000166 GO:0001894 GO:0003824 GO:0003989 GO:0004075 GO:0005524 GO:0005730 GO:0005737 GO:0005739 GO:0005829 GO:0006084 GO:0006629 GO:0006631 GO:0006633 GO:0006768 GO:0006853 GO:0008152 GO:0015629 GO:0016874 GO:0031325 GO:0035338 GO:0044268 GO:0046872 GO:0051289 GO:0055088 GO:0070062 GO:0071380 GO:2001295
5	0.06	0.410	6.27	0.04	0.69	1on3E	GO:0003989 GO:0006633 GO:0009317 GO:0016740 GO:0016874 GO:0047154
6	0.06	0.334	6.70	0.04	0.60	2bzrA	GO:0000166 GO:0003989 GO:0004658 GO:0005524 GO:0005618 GO:0005886 GO:0009317 GO:0015977 GO:0016874 GO:0043234
7	0.06	0.410	5.70	0.05	0.64	4g2rB	GO:0000166 GO:0003989 GO:0004658 GO:0005524 GO:0005618 GO:0009317 GO:0016874 GO:0019367 GO:0040007
8	0.06	0.414	5.95	0.07	0.67	3iavA	GO:0003989 GO:0006633 GO:0009317 GO:0016874
9	0.06	0.405	6.07	0.03	0.67	1x0uA	GO:0003989 GO:0004658 GO:0006633 GO:0009317 GO:0016874
10	0.06	0.358	6.82	0.06	0.66	1acoA	GO:0003994 GO:0005506 GO:0005634 GO:0005739 GO:0006099 GO:0006101 GO:0008152 GO:0016829 GO:0043209 GO:0046872 GO:0051536 GO:0051539
11	0.06	0.413	5.85	0.05	0.66	4rcnB	GO:0003824 GO:0004075 GO:0005524 GO:0016874 GO:0046872
12	0.06	0.427	6.10	0.08	0.71	2x24B
13	0.06	0.357	5.67	0.07	0.56	4rcnA	GO:0003824 GO:0004075 GO:0005524 GO:0016874 GO:0046872
14	0.06	0.434	6.22	0.07	0.73	3ff6A	GO:0000166 GO:0003824 GO:0003989 GO:0004075 GO:0005524 GO:0005634 GO:0005739 GO:0005741 GO:0005829 GO:0006084 GO:0006629 GO:0006631 GO:0006633 GO:0006768 GO:0006853 GO:0008152 GO:0010629 GO:0010884 GO:0010906 GO:0012505 GO:0016020 GO:0016874 GO:0031325 GO:0031667 GO:0031999 GO:0043086 GO:0046322 GO:0046872 GO:0050995 GO:0051289 GO:0060421 GO:0097009 GO:2001295
15	0.06	0.395	6.89	0.06	0.73	3ueqA	GO:0003824 GO:0005576 GO:0005975 GO:0016740 GO:0016757 GO:0047669
16	0.06	0.316	6.34	0.06	0.54	5cs4B	GO:0000166 GO:0003824 GO:0003989 GO:0004075 GO:0005524 GO:0005737 GO:0005783 GO:0005789 GO:0006606 GO:0006629 GO:0006631 GO:0006633 GO:0006998 GO:0008152 GO:0016020 GO:0016874 GO:0042759 GO:0046872 GO:2001295
17	0.06	0.401	5.95	0.03	0.65	1vrgA	GO:0003989 GO:0004658 GO:0016740 GO:0016874
18	0.06	0.339	6.65	0.02	0.61	3n6rB	GO:0004658 GO:0016874

Consensus prediction of GO terms

Molecular Function	GO:0016421	GO:0032559	GO:0035639	GO:0032550	GO:0016879	GO:0043169
GO-Score	0.57	0.57	0.57	0.57	0.47	0.47
Biological Processes	GO:0008610	GO:0071616	GO:2001293	GO:0072330	GO:0044744	GO:0001676	GO:0010256	GO:0017038	GO:0006997	GO:1902593	GO:0044802
GO-Score	0.47	0.47	0.47	0.47	0.36	0.36	0.36	0.36	0.36	0.36	0.36
Cellular Component	GO:0042175	GO:0044432
GO-Score	0.36	0.36

(a)	Cscore^GO is a combined measure for evaluating global and local similarity between query and template protein. It's range is [0-1] and higher values indicate more confident predictions.
(b)	TM-score is a measure of global structural similarity between query and template protein.
(c)	RMSD^a is the RMSD between residues that are structurally aligned by TM-align.
(d)	IDEN^a is the percentage sequence identity in the structurally aligned region.
(e)	Cov represents the coverage of global structural alignment and is equal to the number of structurally aligned residues divided by length of the query protein.
(f)	The second table shows a consensus GO terms amongst the top scoring templates. The GO-Score associated with each prediction is defined as the average weight of the GO term, where the weights are assigned based on Cscore^GO of the template.

[Click on result.tar.bz2 to download the tarball file including all modelling results listed on this page]

Please cite the following articles when you use the I-TASSER server:
1.	J Yang, R Yan, A Roy, D Xu, J Poisson, Y Zhang. The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8, 2015.
2.	J Yang, Y Zhang. I-TASSER server: new development for protein structure and function predictions, Nucleic Acids Research, 43: W174-W181, 2015.
3.	A Roy, A Kucukural, Y Zhang. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738, 2010.
4.	Y Zhang. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, 9: 40, 2008.